fitc conjugated anti c3 antibody  (Valiant)

 
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    Name:
    Anti rabbit complement C3 goat F Ab 2 fragment FITC conjugated
    Description:
    Product is the lyophilized powder of fluorescein 5 isothiocyanate FITC Isomer I conjugated goat IgG fraction to rabbit complement C3 and buffer salts
    Catalog Number:
    0855654
    Price:
    446.25
    Category:
    Life Sciences Antibodies Primary Antibodies
    Applications:
    Immunoassays, Immunofluorescence assays (IFA), Immunoblot
    Size:
    2 mL
    Buy from Supplier


    Structured Review

    Valiant fitc conjugated anti c3 antibody
    Anti rabbit complement C3 goat F Ab 2 fragment FITC conjugated
    Product is the lyophilized powder of fluorescein 5 isothiocyanate FITC Isomer I conjugated goat IgG fraction to rabbit complement C3 and buffer salts
    https://www.bioz.com/result/fitc conjugated anti c3 antibody/product/Valiant
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fitc conjugated anti c3 antibody - by Bioz Stars, 2021-04
    94/100 stars

    Images

    1) Product Images from "Multi-functional mechanisms of immune evasion by the streptococcal complement inhibitor C5a peptidase"

    Article Title: Multi-functional mechanisms of immune evasion by the streptococcal complement inhibitor C5a peptidase

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1006493

    ScpA activity impairs C3 deposition on the GAS surface. A) C3 deposition on GAS-M1 and GAS-M1 ΔscpA surface was compared following incubation with 0% to 50% human serum. Deposition of C3 on the bacterial surface was quantified using FITC-conjugated anti-C3. Data represent mean +/- SD of 3 serum donors. C3 deposition was not seen when 50% heat-inactivated (HI) serum was used. B) GAS-M89 and isogenic ΔscpA strains were incubated with 50% human serum for 30 minutes, at 37°C. Deposition of C3 on the bacterial surface was quantified using FITC-conjugated anti-C3. Data represent mean +/- SD of 3–4 technical replicates produced from 3 serum donors. (One-tailed T-test * = p
    Figure Legend Snippet: ScpA activity impairs C3 deposition on the GAS surface. A) C3 deposition on GAS-M1 and GAS-M1 ΔscpA surface was compared following incubation with 0% to 50% human serum. Deposition of C3 on the bacterial surface was quantified using FITC-conjugated anti-C3. Data represent mean +/- SD of 3 serum donors. C3 deposition was not seen when 50% heat-inactivated (HI) serum was used. B) GAS-M89 and isogenic ΔscpA strains were incubated with 50% human serum for 30 minutes, at 37°C. Deposition of C3 on the bacterial surface was quantified using FITC-conjugated anti-C3. Data represent mean +/- SD of 3–4 technical replicates produced from 3 serum donors. (One-tailed T-test * = p

    Techniques Used: Activity Assay, Incubation, Produced, One-tailed Test

    Related Articles

    other:

    Article Title: Ceramide Kinase-Like (CERKL) Interacts with Neuronal Calcium Sensor Proteins in the Retina in a Cation-Dependent Manner
    Article Snippet: The following secondary antibodies were used: peroxidase-conjugated affiniPure goat anti-mouse IgG, peroxidase-conjugated affiniPure goat anti-rabbit IgG, and peroxidase-conjugated IgG fraction monoclonal mouse anti-rabbit IgG light-chain specific (Jackson ImmunoResearch Laboratories, West Grove, PA); FITC-conjugated goat anti-rabbit IgG (MP Biomedicals, Santa Ana, CA); and protein A-peroxidase (Invitrogen, Grand Island, NY).

    Incubation:

    Article Title: The Core Promoter of the Capsule Operon of Streptococcus pneumoniae Is Necessary for Colonization and Invasive Disease
    Article Snippet: C3 deposition reaction mixtures (150 μl) were comprised of 107 CFU in 50 μl and 100 μl of infant rabbit serum to a final concentration of 10% (AbD Serotec, Co.). .. Samples were incubated in a 37°C rolling incubator for 30 min. Next, opsonization reaction mixtures were chilled for 3 min on ice, quenched with 500 μl of Hanks buffer without Ca2+ and Mg2+ (Gibco) with 0.1% gelatin and pelleted at 4,000 rpm for 5 min. Pellets were resuspended in fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit C3 antibody in 100 μl of Hanks buffer without Ca2+ and Mg2+ with 0.1% gelatin at 1:200 (MP Biomedicals) and incubated on ice in the dark for 30 min. Staining reactions were quenched with 500 μl of Hanks buffer without Ca2+ and Mg2+ with 0.1% gelatin and centrifuged at 4,000 rpm for 5 min, and pellets were resuspended in 300 μl of 2% paraformaldehyde (PFA; Sigma-Aldrich, Co.). .. Samples were collected (25,000 events) on a FACSCalibur analytical flow cytometer.

    Article Title: Upregulated expression of FGF13/FHF2 mediates resistance to platinum drugs in cervical cancer cells
    Article Snippet: The fixed cells were washed three times with phosphate buffered saline containing 0.1% sodium azide and 0.1% bovine serum albumin, and were incubated with affinity-purified rabbit polyclonal anti-FGF13 antibody on ice for 60 min. For negative control samples, the cells were not incubated with the primary antibody. .. After washing, the cells (1 × 106 ) were incubated with 5 μg/ml fluorescein isothiocyanate (FITC)-conjugated F (ab′)2 goat anti-rabbit IgG (MP Biomedicals, Solon, OH) on ice for 30 min. After washing, the cells were subjected to flow cytometric analysis (Epics Elite, Beckman-Coulter Inc., Fullerton, CA). ..

    Article Title: Improved glucose metabolism by Eragrostis tef potentially through beige adipocyte formation and attenuating adipose tissue inflammation
    Article Snippet: Immunofluorescence staining: tissue sections were then incubated overnight at 4°C with a 1:50 dilution of primary antibodies (anti-F4/80, MCA497, Bio-Rad, Hercules, CA, USA; anti-TNFα, ab6671, Abcam, Cambridge, MA, USA; anti-Perilipin, 9349S, Cell Signalling Technology, Danvers, MA, USA). .. Sections were then incubated with a 1:1000 dilution of secondary antibodies for 1h at room temperature [rhodamine-conjugated anti-rat secondary antibody (Cappel, MP Biomedicals, LLC, Solon, OH, USA) for F4/80 and FITC-conjugated anti-rabbit (Cappel, MP Biomedicals, LLC) for TNFα and Perilipin)]. .. Nuclei were stained with DAPI.

    Staining:

    Article Title: The Core Promoter of the Capsule Operon of Streptococcus pneumoniae Is Necessary for Colonization and Invasive Disease
    Article Snippet: C3 deposition reaction mixtures (150 μl) were comprised of 107 CFU in 50 μl and 100 μl of infant rabbit serum to a final concentration of 10% (AbD Serotec, Co.). .. Samples were incubated in a 37°C rolling incubator for 30 min. Next, opsonization reaction mixtures were chilled for 3 min on ice, quenched with 500 μl of Hanks buffer without Ca2+ and Mg2+ (Gibco) with 0.1% gelatin and pelleted at 4,000 rpm for 5 min. Pellets were resuspended in fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit C3 antibody in 100 μl of Hanks buffer without Ca2+ and Mg2+ with 0.1% gelatin at 1:200 (MP Biomedicals) and incubated on ice in the dark for 30 min. Staining reactions were quenched with 500 μl of Hanks buffer without Ca2+ and Mg2+ with 0.1% gelatin and centrifuged at 4,000 rpm for 5 min, and pellets were resuspended in 300 μl of 2% paraformaldehyde (PFA; Sigma-Aldrich, Co.). .. Samples were collected (25,000 events) on a FACSCalibur analytical flow cytometer.

    Article Title: Multi-functional mechanisms of immune evasion by the streptococcal complement inhibitor C5a peptidase
    Article Snippet: Control samples were incubated with fresh heat-inactivated human serum and human or mouse C3-depleted serum (EMD Millipore). .. Cells were stained with FITC-conjugated anti-C3 antibody (MP Biomedicals) for 30 minutes on ice [ ] and analyzed using a FACSCalibur cell analyzer (BD Biosciences) and FlowJo Software (Treestar, OR). .. In order to combine the percentage of C3-positive bacteria and the C3-binding intensity, the results are presented as fluorescence index (FI), which is calculated as the proportion of positive bacteria expressed as a percentage multiplied by the gMFI) [ ].

    Article Title: Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy
    Article Snippet: The reaction was stopped with 100 μl GVB/EDTA (Complement Technology) +1x FUT-175 (BD). .. POS were washed 2x in FACS buffer (PBS/0.5% BSA/2 mM EDTA) and stained with either 1 μg/ml mouse-anti-C9 neo-epitope (Hycult clone aE11), mouse-anti-C4d (Quidel), or 1:400 FITC-conjugated goat-anti-C3 (MP Biomedicals) followed by anti-mouse-A647 (Jackson Immunoresearch). .. Flow cytometry was performed on a FACS Calibur (BD) and analyzed in FlowJo.

    Flow Cytometry:

    Article Title: Upregulated expression of FGF13/FHF2 mediates resistance to platinum drugs in cervical cancer cells
    Article Snippet: The fixed cells were washed three times with phosphate buffered saline containing 0.1% sodium azide and 0.1% bovine serum albumin, and were incubated with affinity-purified rabbit polyclonal anti-FGF13 antibody on ice for 60 min. For negative control samples, the cells were not incubated with the primary antibody. .. After washing, the cells (1 × 106 ) were incubated with 5 μg/ml fluorescein isothiocyanate (FITC)-conjugated F (ab′)2 goat anti-rabbit IgG (MP Biomedicals, Solon, OH) on ice for 30 min. After washing, the cells were subjected to flow cytometric analysis (Epics Elite, Beckman-Coulter Inc., Fullerton, CA). ..

    Software:

    Article Title: Multi-functional mechanisms of immune evasion by the streptococcal complement inhibitor C5a peptidase
    Article Snippet: Control samples were incubated with fresh heat-inactivated human serum and human or mouse C3-depleted serum (EMD Millipore). .. Cells were stained with FITC-conjugated anti-C3 antibody (MP Biomedicals) for 30 minutes on ice [ ] and analyzed using a FACSCalibur cell analyzer (BD Biosciences) and FlowJo Software (Treestar, OR). .. In order to combine the percentage of C3-positive bacteria and the C3-binding intensity, the results are presented as fluorescence index (FI), which is calculated as the proportion of positive bacteria expressed as a percentage multiplied by the gMFI) [ ].

    FACS:

    Article Title: Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy
    Article Snippet: The reaction was stopped with 100 μl GVB/EDTA (Complement Technology) +1x FUT-175 (BD). .. POS were washed 2x in FACS buffer (PBS/0.5% BSA/2 mM EDTA) and stained with either 1 μg/ml mouse-anti-C9 neo-epitope (Hycult clone aE11), mouse-anti-C4d (Quidel), or 1:400 FITC-conjugated goat-anti-C3 (MP Biomedicals) followed by anti-mouse-A647 (Jackson Immunoresearch). .. Flow cytometry was performed on a FACS Calibur (BD) and analyzed in FlowJo.

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  • About
  • News
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  • Team
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  • Contact
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  • 94
    Valiant fitc conjugated anti c3 antibody
    ScpA activity impairs C3 deposition on the GAS surface. A) C3 deposition on GAS-M1 and GAS-M1 ΔscpA surface was compared following incubation with 0% to 50% human serum. Deposition of C3 on the bacterial surface was quantified using <t>FITC-conjugated</t> <t>anti-C3.</t> Data represent mean +/- SD of 3 serum donors. C3 deposition was not seen when 50% heat-inactivated (HI) serum was used. B) GAS-M89 and isogenic ΔscpA strains were incubated with 50% human serum for 30 minutes, at 37°C. Deposition of C3 on the bacterial surface was quantified using FITC-conjugated anti-C3. Data represent mean +/- SD of 3–4 technical replicates produced from 3 serum donors. (One-tailed T-test * = p
    Fitc Conjugated Anti C3 Antibody, supplied by Valiant, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated anti c3 antibody/product/Valiant
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fitc conjugated anti c3 antibody - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

    Image Search Results


    ScpA activity impairs C3 deposition on the GAS surface. A) C3 deposition on GAS-M1 and GAS-M1 ΔscpA surface was compared following incubation with 0% to 50% human serum. Deposition of C3 on the bacterial surface was quantified using FITC-conjugated anti-C3. Data represent mean +/- SD of 3 serum donors. C3 deposition was not seen when 50% heat-inactivated (HI) serum was used. B) GAS-M89 and isogenic ΔscpA strains were incubated with 50% human serum for 30 minutes, at 37°C. Deposition of C3 on the bacterial surface was quantified using FITC-conjugated anti-C3. Data represent mean +/- SD of 3–4 technical replicates produced from 3 serum donors. (One-tailed T-test * = p

    Journal: PLoS Pathogens

    Article Title: Multi-functional mechanisms of immune evasion by the streptococcal complement inhibitor C5a peptidase

    doi: 10.1371/journal.ppat.1006493

    Figure Lengend Snippet: ScpA activity impairs C3 deposition on the GAS surface. A) C3 deposition on GAS-M1 and GAS-M1 ΔscpA surface was compared following incubation with 0% to 50% human serum. Deposition of C3 on the bacterial surface was quantified using FITC-conjugated anti-C3. Data represent mean +/- SD of 3 serum donors. C3 deposition was not seen when 50% heat-inactivated (HI) serum was used. B) GAS-M89 and isogenic ΔscpA strains were incubated with 50% human serum for 30 minutes, at 37°C. Deposition of C3 on the bacterial surface was quantified using FITC-conjugated anti-C3. Data represent mean +/- SD of 3–4 technical replicates produced from 3 serum donors. (One-tailed T-test * = p

    Article Snippet: Cells were stained with FITC-conjugated anti-C3 antibody (MP Biomedicals) for 30 minutes on ice [ ] and analyzed using a FACSCalibur cell analyzer (BD Biosciences) and FlowJo Software (Treestar, OR).

    Techniques: Activity Assay, Incubation, Produced, One-tailed Test