rabbit anti mouse igg  (Valiant)

 
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    Name:
    Anti mouse IgG whole molecule rabbit antiserum
    Description:
    Product is the lyophilized powder of rabbit antiserum to mouse IgG whole molecule and buffer salts
    Catalog Number:
    0855436
    Price:
    95.25
    Category:
    Life Sciences Antibodies Antisera
    Applications:
    Immunoassays
    Size:
    2 mL
    Buy from Supplier


    Structured Review

    Valiant rabbit anti mouse igg
    Anti mouse IgG whole molecule rabbit antiserum
    Product is the lyophilized powder of rabbit antiserum to mouse IgG whole molecule and buffer salts
    https://www.bioz.com/result/rabbit anti mouse igg/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mouse igg - by Bioz Stars, 2021-04
    93/100 stars

    Images

    1) Product Images from "Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression"

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20121716

    The RB family controls the expression of Foxn1 . (A) Expression of Foxn1 in CD45 – MHCII + TECS from control (Ctrl) and mutant (Mut) mice relative to Eva1 expression ( n = 4; P = 0.03). (B) Immunoblot analysis of Foxn1 levels in control and mutant TECs from 12 pooled control and 5 pooled mutant thymi. α-Tubulin is included as a loading control ( n = 2). (C) Immunofluorescence staining of Foxn1 and CD205 individually and merged (right) in control (top) and mutant (bottom) thymi (one of two shown). Bars, 100 µm. (D) Schematic representation of the Foxn1 promoter. Region 1a is active in the skin and thymus, whereas region 1b is only active in the skin. (E) Sequence alignment on the UCSC genome browser ( Fujita et al., 2011 ) of the promoter region 1a encompassing two E2F sites shows conservation across multiple mammalian species ( Kent, 2002 ). Gray highlighting indicates conserved sequences, and black highlighting indicates putative E2F binding sites. (F) Transfection into 210R TECs of an expression plasmid coding for E2F3 together with a luciferase reporter plasmid for either the wild-type region 1a of the Foxn1 promoter or a region 1a where site 3 has been mutated ( n = 3; P = 0.03). (G) Transfection into 210R cells of E2F3 and RB expression and the Foxn1 luciferase reporter plasmid ( n = 3; P = 0.02). (H) ChIP analysis of E2F3 and E2F4 to sites 1 and 2–3 in TEC100 cells. To adjust for inter-experiment variability of scale of percent input, experiments were normalized to nonspecific antibodies (IgG and anti-p16 INK4a ). This ratio is represented here. In addition, binding to a nonspecific region of the fifth chromosome was included as a negative control ( n = 3; for site 1 P = 0.02, for site 2–3 P = 0.04). (I) Fold change in percent input as assessed by ChIP analysis of E2F3 and E2F4 binding to the Foxn1 promoter in TEC100 cells after transient overexpression of RB ( n = 3 compared with untransfected controls; p-values: E2F3 Foxn1 site 1 = 0.06 and site 2–3 = 0.04; E2F4 Foxn1 site 1 = 0.01 and site 2–3 = 0.02). Asterisks indicate P
    Figure Legend Snippet: The RB family controls the expression of Foxn1 . (A) Expression of Foxn1 in CD45 – MHCII + TECS from control (Ctrl) and mutant (Mut) mice relative to Eva1 expression ( n = 4; P = 0.03). (B) Immunoblot analysis of Foxn1 levels in control and mutant TECs from 12 pooled control and 5 pooled mutant thymi. α-Tubulin is included as a loading control ( n = 2). (C) Immunofluorescence staining of Foxn1 and CD205 individually and merged (right) in control (top) and mutant (bottom) thymi (one of two shown). Bars, 100 µm. (D) Schematic representation of the Foxn1 promoter. Region 1a is active in the skin and thymus, whereas region 1b is only active in the skin. (E) Sequence alignment on the UCSC genome browser ( Fujita et al., 2011 ) of the promoter region 1a encompassing two E2F sites shows conservation across multiple mammalian species ( Kent, 2002 ). Gray highlighting indicates conserved sequences, and black highlighting indicates putative E2F binding sites. (F) Transfection into 210R TECs of an expression plasmid coding for E2F3 together with a luciferase reporter plasmid for either the wild-type region 1a of the Foxn1 promoter or a region 1a where site 3 has been mutated ( n = 3; P = 0.03). (G) Transfection into 210R cells of E2F3 and RB expression and the Foxn1 luciferase reporter plasmid ( n = 3; P = 0.02). (H) ChIP analysis of E2F3 and E2F4 to sites 1 and 2–3 in TEC100 cells. To adjust for inter-experiment variability of scale of percent input, experiments were normalized to nonspecific antibodies (IgG and anti-p16 INK4a ). This ratio is represented here. In addition, binding to a nonspecific region of the fifth chromosome was included as a negative control ( n = 3; for site 1 P = 0.02, for site 2–3 P = 0.04). (I) Fold change in percent input as assessed by ChIP analysis of E2F3 and E2F4 binding to the Foxn1 promoter in TEC100 cells after transient overexpression of RB ( n = 3 compared with untransfected controls; p-values: E2F3 Foxn1 site 1 = 0.06 and site 2–3 = 0.04; E2F4 Foxn1 site 1 = 0.01 and site 2–3 = 0.02). Asterisks indicate P

    Techniques Used: Expressing, Mutagenesis, Mouse Assay, Immunofluorescence, Staining, Sequencing, Binding Assay, Transfection, Plasmid Preparation, Luciferase, Chromatin Immunoprecipitation, Negative Control, Over Expression

    2) Product Images from "Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity"

    Article Title: Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0063269

    Antibodies titers in mice after immunization with NoV VLPs, P particles, and P dimers. VA387 VLPs, P particles, P dimers were administrated to the mice (30 µg/mouse, n = 5) intranasally without adjuvant for three times in a two-week interval. Mice administered with PBS were regarded as negative controls (Ctrl). Sera were collected from each mouse prior to the first and one week after the final immunization. The titers of NoV-specific total IgG (A) and its subtypes IgG1 (B), IgG2a (C) and IgG2b (D) were determined by ELISA against purified P dimer. The antibody titers were defined as the endpoint dilution with a cut off signal intensity of 0.2. ∗ P
    Figure Legend Snippet: Antibodies titers in mice after immunization with NoV VLPs, P particles, and P dimers. VA387 VLPs, P particles, P dimers were administrated to the mice (30 µg/mouse, n = 5) intranasally without adjuvant for three times in a two-week interval. Mice administered with PBS were regarded as negative controls (Ctrl). Sera were collected from each mouse prior to the first and one week after the final immunization. The titers of NoV-specific total IgG (A) and its subtypes IgG1 (B), IgG2a (C) and IgG2b (D) were determined by ELISA against purified P dimer. The antibody titers were defined as the endpoint dilution with a cut off signal intensity of 0.2. ∗ P

    Techniques Used: Mouse Assay, Enzyme-linked Immunosorbent Assay, Purification

    Related Articles

    Purification:

    Article Title: Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity
    Article Snippet: .. Reagents Various reagents were purchased from following companies: mouse monoclonal antibodies anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53–6.7), anti-CD44 (IM7), anti-CD62L (MEL-14), anti-CCR7 (4B12), anti-CD11c (N418), anti-CD40 (3/23), anti-CD80 (16-10A1), anti-CD86 (GL-1), anti-I-Ab (AF6-120.1), anti-IL-2 (JES6-5H4), anti-IFN-γ (XMG1.2), anti-TNF-α (MP6-XT22) from Biolegend (San Diego, CA), purified anti-mouse IL-12 p70 (C18.2) and biotin anti-mouse IL-12/IL-23 p40 (C17.8) from Biolegend, purified anti-mouse IFN-γ (R4-6A2) and biotin anti-mouse IFN-γ (XMG1.2) from Biolegend, ELISA set of murine IL-1β from BD Bioscience (San Jose, CA), 10x RBC lysis buffer and fixation/permeabilization buffers from eBioscience (San Diego, CA), Alexa Fluor 488® protein labeling kit from Invitrogen (Grand Island, NY), HRP-conjugated goat anti-mouse IgG, IgG1 and rabbit anti-mouse IgG2a, 2b from MP Biomedicals (Solon, OH, USA), and Polymyxin B from Sigma-Aldrich (St. Louis, MO). .. CD4+ T cell epitope (FYQEAAPAQSDVAL) targeting NoV GII.4 strain VA387 were predicted based on T cell epitope prediction tools from Immune Epitope Database (IEDB) Analysis Resource (website: http://tools.immuneepitope.org/main/ ) and synthesized by GenScript (Piscataway, NJ).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity
    Article Snippet: .. Reagents Various reagents were purchased from following companies: mouse monoclonal antibodies anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53–6.7), anti-CD44 (IM7), anti-CD62L (MEL-14), anti-CCR7 (4B12), anti-CD11c (N418), anti-CD40 (3/23), anti-CD80 (16-10A1), anti-CD86 (GL-1), anti-I-Ab (AF6-120.1), anti-IL-2 (JES6-5H4), anti-IFN-γ (XMG1.2), anti-TNF-α (MP6-XT22) from Biolegend (San Diego, CA), purified anti-mouse IL-12 p70 (C18.2) and biotin anti-mouse IL-12/IL-23 p40 (C17.8) from Biolegend, purified anti-mouse IFN-γ (R4-6A2) and biotin anti-mouse IFN-γ (XMG1.2) from Biolegend, ELISA set of murine IL-1β from BD Bioscience (San Jose, CA), 10x RBC lysis buffer and fixation/permeabilization buffers from eBioscience (San Diego, CA), Alexa Fluor 488® protein labeling kit from Invitrogen (Grand Island, NY), HRP-conjugated goat anti-mouse IgG, IgG1 and rabbit anti-mouse IgG2a, 2b from MP Biomedicals (Solon, OH, USA), and Polymyxin B from Sigma-Aldrich (St. Louis, MO). .. CD4+ T cell epitope (FYQEAAPAQSDVAL) targeting NoV GII.4 strain VA387 were predicted based on T cell epitope prediction tools from Immune Epitope Database (IEDB) Analysis Resource (website: http://tools.immuneepitope.org/main/ ) and synthesized by GenScript (Piscataway, NJ).

    Lysis:

    Article Title: Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity
    Article Snippet: .. Reagents Various reagents were purchased from following companies: mouse monoclonal antibodies anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53–6.7), anti-CD44 (IM7), anti-CD62L (MEL-14), anti-CCR7 (4B12), anti-CD11c (N418), anti-CD40 (3/23), anti-CD80 (16-10A1), anti-CD86 (GL-1), anti-I-Ab (AF6-120.1), anti-IL-2 (JES6-5H4), anti-IFN-γ (XMG1.2), anti-TNF-α (MP6-XT22) from Biolegend (San Diego, CA), purified anti-mouse IL-12 p70 (C18.2) and biotin anti-mouse IL-12/IL-23 p40 (C17.8) from Biolegend, purified anti-mouse IFN-γ (R4-6A2) and biotin anti-mouse IFN-γ (XMG1.2) from Biolegend, ELISA set of murine IL-1β from BD Bioscience (San Jose, CA), 10x RBC lysis buffer and fixation/permeabilization buffers from eBioscience (San Diego, CA), Alexa Fluor 488® protein labeling kit from Invitrogen (Grand Island, NY), HRP-conjugated goat anti-mouse IgG, IgG1 and rabbit anti-mouse IgG2a, 2b from MP Biomedicals (Solon, OH, USA), and Polymyxin B from Sigma-Aldrich (St. Louis, MO). .. CD4+ T cell epitope (FYQEAAPAQSDVAL) targeting NoV GII.4 strain VA387 were predicted based on T cell epitope prediction tools from Immune Epitope Database (IEDB) Analysis Resource (website: http://tools.immuneepitope.org/main/ ) and synthesized by GenScript (Piscataway, NJ).

    Labeling:

    Article Title: Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity
    Article Snippet: .. Reagents Various reagents were purchased from following companies: mouse monoclonal antibodies anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53–6.7), anti-CD44 (IM7), anti-CD62L (MEL-14), anti-CCR7 (4B12), anti-CD11c (N418), anti-CD40 (3/23), anti-CD80 (16-10A1), anti-CD86 (GL-1), anti-I-Ab (AF6-120.1), anti-IL-2 (JES6-5H4), anti-IFN-γ (XMG1.2), anti-TNF-α (MP6-XT22) from Biolegend (San Diego, CA), purified anti-mouse IL-12 p70 (C18.2) and biotin anti-mouse IL-12/IL-23 p40 (C17.8) from Biolegend, purified anti-mouse IFN-γ (R4-6A2) and biotin anti-mouse IFN-γ (XMG1.2) from Biolegend, ELISA set of murine IL-1β from BD Bioscience (San Jose, CA), 10x RBC lysis buffer and fixation/permeabilization buffers from eBioscience (San Diego, CA), Alexa Fluor 488® protein labeling kit from Invitrogen (Grand Island, NY), HRP-conjugated goat anti-mouse IgG, IgG1 and rabbit anti-mouse IgG2a, 2b from MP Biomedicals (Solon, OH, USA), and Polymyxin B from Sigma-Aldrich (St. Louis, MO). .. CD4+ T cell epitope (FYQEAAPAQSDVAL) targeting NoV GII.4 strain VA387 were predicted based on T cell epitope prediction tools from Immune Epitope Database (IEDB) Analysis Resource (website: http://tools.immuneepitope.org/main/ ) and synthesized by GenScript (Piscataway, NJ).

    Incubation:

    Article Title: A Conserved Organization of Transcription during Embryonic Stem Cell Differentiation and in Cells with High C Value
    Article Snippet: The estimated average volume of 10 fluorescent microspheres (6 and 15 μm in diameter; Molecular Probes) lay within 1% of the real volume. .. Br-RNA in cryosections on grids was incubated with the primary anti-BrdU, washed (all as described above) and incubated with either 1) goat anti-mouse IgG conjugated with 5-nm gold particles (1 μg/ml; British Biocell International, Cardiff, United Kingdom) for 3–4 h or 2) rabbit anti-mouse IgG (13 μg/ml; MP Biomedicals, Irvine, CA) for 1 h followed by goat anti-rabbit IgG conjugated with 5-nm particles (1 μg/ml; British Biocell International) for 3–4 h. Because double and triple sandwiches gave similar site densities and diameters, results were pooled. .. After the final antibody, cryosections were washed (4 times over 40 min) in PBS+, rewashed (16 h; 4°C) in PBS and then in water (5 drops; 1 h), and stained (10 min; 4°C) with 0.3% uranyl acetate in 2% methylcellulose.

    Article Title: Diverse molecular mechanisms contribute to differential expression of human duplicated genes
    Article Snippet: ChIP enrichment was performed by incubation for 16 h at 4°C with the following antibodies: 2 μg H3K27ac antibody (Active Motif #39133), 4 μg H3K4me1 antibody (Millipore 07-436), 2 μg H3K4me3 antibody (Active Motif #39915), or 2 μg RNA Polymerase II (PolII) antibody clone 8WG16 (Covance MMS-126R). .. RNA PolII samples were incubated for an additional hour with 2 μg Rabbit Anti-Mouse IgG (MP Biomedical #55436). .. Immune complexes were bound to 20 μl magnetic protein A/G beads (ThermoFisher) for 2 hours at 4°C.

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Chromatin was sonicated using a fisher probe sonicator 30% output power for eight cycles of 30 s. The chromatin was precleared before being diluted and bound by 4 µg of the primary antibody overnight at 4°C. .. Each ChIP was then incubated with 8 µg rabbit anti–mouse IgG (MP Biomedicals) or anti–mouse p16 antibody (N-20; Santa Cruz Biotechnology, Inc.) as a secondary for 1 h. Nucleoprotein complexes were pulled down using Pansorbin cells (EMD Millipore). .. Complexes were digested with Proteinase K and RNase A and purified by a QIAquick PCR Purification kit (QIAGEN).

    Chromatin Immunoprecipitation:

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression
    Article Snippet: Chromatin was sonicated using a fisher probe sonicator 30% output power for eight cycles of 30 s. The chromatin was precleared before being diluted and bound by 4 µg of the primary antibody overnight at 4°C. .. Each ChIP was then incubated with 8 µg rabbit anti–mouse IgG (MP Biomedicals) or anti–mouse p16 antibody (N-20; Santa Cruz Biotechnology, Inc.) as a secondary for 1 h. Nucleoprotein complexes were pulled down using Pansorbin cells (EMD Millipore). .. Complexes were digested with Proteinase K and RNase A and purified by a QIAquick PCR Purification kit (QIAGEN).

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    Valiant rabbit anti mouse igg
    The RB family controls the expression of Foxn1 . (A) Expression of Foxn1 in CD45 – MHCII + TECS from control (Ctrl) and mutant (Mut) mice relative to Eva1 expression ( n = 4; P = 0.03). (B) Immunoblot analysis of Foxn1 levels in control and mutant TECs from 12 pooled control and 5 pooled mutant thymi. α-Tubulin is included as a loading control ( n = 2). (C) Immunofluorescence staining of Foxn1 and CD205 individually and merged (right) in control (top) and mutant (bottom) thymi (one of two shown). Bars, 100 µm. (D) Schematic representation of the Foxn1 promoter. Region 1a is active in the skin and thymus, whereas region 1b is only active in the skin. (E) Sequence alignment on the UCSC genome browser ( Fujita et al., 2011 ) of the promoter region 1a encompassing two E2F sites shows conservation across multiple mammalian species ( Kent, 2002 ). Gray highlighting indicates conserved sequences, and black highlighting indicates putative E2F binding sites. (F) Transfection into 210R TECs of an expression plasmid coding for E2F3 together with a luciferase reporter plasmid for either the wild-type region 1a of the Foxn1 promoter or a region 1a where site 3 has been mutated ( n = 3; P = 0.03). (G) Transfection into 210R cells of E2F3 and RB expression and the Foxn1 luciferase reporter plasmid ( n = 3; P = 0.02). (H) ChIP analysis of E2F3 and E2F4 to sites 1 and 2–3 in TEC100 cells. To adjust for inter-experiment variability of scale of percent input, experiments were normalized to nonspecific antibodies <t>(IgG</t> and <t>anti-p16</t> INK4a ). This ratio is represented here. In addition, binding to a nonspecific region of the fifth chromosome was included as a negative control ( n = 3; for site 1 P = 0.02, for site 2–3 P = 0.04). (I) Fold change in percent input as assessed by ChIP analysis of E2F3 and E2F4 binding to the Foxn1 promoter in TEC100 cells after transient overexpression of RB ( n = 3 compared with untransfected controls; p-values: E2F3 Foxn1 site 1 = 0.06 and site 2–3 = 0.04; E2F4 Foxn1 site 1 = 0.01 and site 2–3 = 0.02). Asterisks indicate P
    Rabbit Anti Mouse Igg, supplied by Valiant, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse igg/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mouse igg - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    The RB family controls the expression of Foxn1 . (A) Expression of Foxn1 in CD45 – MHCII + TECS from control (Ctrl) and mutant (Mut) mice relative to Eva1 expression ( n = 4; P = 0.03). (B) Immunoblot analysis of Foxn1 levels in control and mutant TECs from 12 pooled control and 5 pooled mutant thymi. α-Tubulin is included as a loading control ( n = 2). (C) Immunofluorescence staining of Foxn1 and CD205 individually and merged (right) in control (top) and mutant (bottom) thymi (one of two shown). Bars, 100 µm. (D) Schematic representation of the Foxn1 promoter. Region 1a is active in the skin and thymus, whereas region 1b is only active in the skin. (E) Sequence alignment on the UCSC genome browser ( Fujita et al., 2011 ) of the promoter region 1a encompassing two E2F sites shows conservation across multiple mammalian species ( Kent, 2002 ). Gray highlighting indicates conserved sequences, and black highlighting indicates putative E2F binding sites. (F) Transfection into 210R TECs of an expression plasmid coding for E2F3 together with a luciferase reporter plasmid for either the wild-type region 1a of the Foxn1 promoter or a region 1a where site 3 has been mutated ( n = 3; P = 0.03). (G) Transfection into 210R cells of E2F3 and RB expression and the Foxn1 luciferase reporter plasmid ( n = 3; P = 0.02). (H) ChIP analysis of E2F3 and E2F4 to sites 1 and 2–3 in TEC100 cells. To adjust for inter-experiment variability of scale of percent input, experiments were normalized to nonspecific antibodies (IgG and anti-p16 INK4a ). This ratio is represented here. In addition, binding to a nonspecific region of the fifth chromosome was included as a negative control ( n = 3; for site 1 P = 0.02, for site 2–3 P = 0.04). (I) Fold change in percent input as assessed by ChIP analysis of E2F3 and E2F4 binding to the Foxn1 promoter in TEC100 cells after transient overexpression of RB ( n = 3 compared with untransfected controls; p-values: E2F3 Foxn1 site 1 = 0.06 and site 2–3 = 0.04; E2F4 Foxn1 site 1 = 0.01 and site 2–3 = 0.02). Asterisks indicate P

    Journal: The Journal of Experimental Medicine

    Article Title: Inactivation of the RB family prevents thymus involution and promotes thymic function by direct control of Foxn1 expression

    doi: 10.1084/jem.20121716

    Figure Lengend Snippet: The RB family controls the expression of Foxn1 . (A) Expression of Foxn1 in CD45 – MHCII + TECS from control (Ctrl) and mutant (Mut) mice relative to Eva1 expression ( n = 4; P = 0.03). (B) Immunoblot analysis of Foxn1 levels in control and mutant TECs from 12 pooled control and 5 pooled mutant thymi. α-Tubulin is included as a loading control ( n = 2). (C) Immunofluorescence staining of Foxn1 and CD205 individually and merged (right) in control (top) and mutant (bottom) thymi (one of two shown). Bars, 100 µm. (D) Schematic representation of the Foxn1 promoter. Region 1a is active in the skin and thymus, whereas region 1b is only active in the skin. (E) Sequence alignment on the UCSC genome browser ( Fujita et al., 2011 ) of the promoter region 1a encompassing two E2F sites shows conservation across multiple mammalian species ( Kent, 2002 ). Gray highlighting indicates conserved sequences, and black highlighting indicates putative E2F binding sites. (F) Transfection into 210R TECs of an expression plasmid coding for E2F3 together with a luciferase reporter plasmid for either the wild-type region 1a of the Foxn1 promoter or a region 1a where site 3 has been mutated ( n = 3; P = 0.03). (G) Transfection into 210R cells of E2F3 and RB expression and the Foxn1 luciferase reporter plasmid ( n = 3; P = 0.02). (H) ChIP analysis of E2F3 and E2F4 to sites 1 and 2–3 in TEC100 cells. To adjust for inter-experiment variability of scale of percent input, experiments were normalized to nonspecific antibodies (IgG and anti-p16 INK4a ). This ratio is represented here. In addition, binding to a nonspecific region of the fifth chromosome was included as a negative control ( n = 3; for site 1 P = 0.02, for site 2–3 P = 0.04). (I) Fold change in percent input as assessed by ChIP analysis of E2F3 and E2F4 binding to the Foxn1 promoter in TEC100 cells after transient overexpression of RB ( n = 3 compared with untransfected controls; p-values: E2F3 Foxn1 site 1 = 0.06 and site 2–3 = 0.04; E2F4 Foxn1 site 1 = 0.01 and site 2–3 = 0.02). Asterisks indicate P

    Article Snippet: Each ChIP was then incubated with 8 µg rabbit anti–mouse IgG (MP Biomedicals) or anti–mouse p16 antibody (N-20; Santa Cruz Biotechnology, Inc.) as a secondary for 1 h. Nucleoprotein complexes were pulled down using Pansorbin cells (EMD Millipore).

    Techniques: Expressing, Mutagenesis, Mouse Assay, Immunofluorescence, Staining, Sequencing, Binding Assay, Transfection, Plasmid Preparation, Luciferase, Chromatin Immunoprecipitation, Negative Control, Over Expression

    Antibodies titers in mice after immunization with NoV VLPs, P particles, and P dimers. VA387 VLPs, P particles, P dimers were administrated to the mice (30 µg/mouse, n = 5) intranasally without adjuvant for three times in a two-week interval. Mice administered with PBS were regarded as negative controls (Ctrl). Sera were collected from each mouse prior to the first and one week after the final immunization. The titers of NoV-specific total IgG (A) and its subtypes IgG1 (B), IgG2a (C) and IgG2b (D) were determined by ELISA against purified P dimer. The antibody titers were defined as the endpoint dilution with a cut off signal intensity of 0.2. ∗ P

    Journal: PLoS ONE

    Article Title: Norovirus P Particle Efficiently Elicits Innate, Humoral and Cellular Immunity

    doi: 10.1371/journal.pone.0063269

    Figure Lengend Snippet: Antibodies titers in mice after immunization with NoV VLPs, P particles, and P dimers. VA387 VLPs, P particles, P dimers were administrated to the mice (30 µg/mouse, n = 5) intranasally without adjuvant for three times in a two-week interval. Mice administered with PBS were regarded as negative controls (Ctrl). Sera were collected from each mouse prior to the first and one week after the final immunization. The titers of NoV-specific total IgG (A) and its subtypes IgG1 (B), IgG2a (C) and IgG2b (D) were determined by ELISA against purified P dimer. The antibody titers were defined as the endpoint dilution with a cut off signal intensity of 0.2. ∗ P

    Article Snippet: Reagents Various reagents were purchased from following companies: mouse monoclonal antibodies anti-CD3 (145-2C11), anti-CD4 (GK1.5), anti-CD8 (53–6.7), anti-CD44 (IM7), anti-CD62L (MEL-14), anti-CCR7 (4B12), anti-CD11c (N418), anti-CD40 (3/23), anti-CD80 (16-10A1), anti-CD86 (GL-1), anti-I-Ab (AF6-120.1), anti-IL-2 (JES6-5H4), anti-IFN-γ (XMG1.2), anti-TNF-α (MP6-XT22) from Biolegend (San Diego, CA), purified anti-mouse IL-12 p70 (C18.2) and biotin anti-mouse IL-12/IL-23 p40 (C17.8) from Biolegend, purified anti-mouse IFN-γ (R4-6A2) and biotin anti-mouse IFN-γ (XMG1.2) from Biolegend, ELISA set of murine IL-1β from BD Bioscience (San Jose, CA), 10x RBC lysis buffer and fixation/permeabilization buffers from eBioscience (San Diego, CA), Alexa Fluor 488® protein labeling kit from Invitrogen (Grand Island, NY), HRP-conjugated goat anti-mouse IgG, IgG1 and rabbit anti-mouse IgG2a, 2b from MP Biomedicals (Solon, OH, USA), and Polymyxin B from Sigma-Aldrich (St. Louis, MO).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Purification