ciprofloxacin  (Valiant)

 
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    Name:
    Thermolysin
    Description:
    This lyophilized enzyme is fromBacillus thermoproteolyticus This enzyme is characterized by its excellent heat stability and substrate specificity towards isoleucine methionine and valine Activity 7000 PU mg of protein
    Catalog Number:
    08321351
    Price:
    722.45
    Category:
    Life Sciences Biochemicals Proteins and Derivatives Enzymes
    Applications:
    Cell Biology Analysis
    Size:
    250 mg
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    Structured Review

    Valiant ciprofloxacin
    The influence of depolymerase, <t>ciprofloxacin,</t> and infective phage KP34 on K. pneumoniae 77 biofilm formed for 72 h on polystyrene pegs. Three - day biofilm was treated for 2 h with: recombinant depolymerase KP34p57 originated from phage KP34 marked as depo, at concentrations corresponding to 1000 MHF (minimal halo forming concentration), 100 MHF, 10 MHF, and 1 MHF, green bars; ciprofloxacin marked as cip, at concentrations corresponding to 4 MIC (minimal inhibitory concentration), 2 MIC, 1 MIC, and 0.5 MIC, yellow bars; and lytic phage KP34 marked as KP34 (10 9 PFU/ml and 10 6 PFU/ml, violet bars). Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P -value
    This lyophilized enzyme is fromBacillus thermoproteolyticus This enzyme is characterized by its excellent heat stability and substrate specificity towards isoleucine methionine and valine Activity 7000 PU mg of protein
    https://www.bioz.com/result/ciprofloxacin/product/Valiant
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ciprofloxacin - by Bioz Stars, 2021-03
    94/100 stars

    Images

    1) Product Images from "Advantages and limitations of microtiter biofilm assays in the model of antibiofilm activity of Klebsiella phage KP34 and its depolymerase"

    Article Title: Advantages and limitations of microtiter biofilm assays in the model of antibiofilm activity of Klebsiella phage KP34 and its depolymerase

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-77198-5

    The influence of depolymerase, ciprofloxacin, and infective phage KP34 on K. pneumoniae 77 biofilm formed for 72 h on polystyrene pegs. Three - day biofilm was treated for 2 h with: recombinant depolymerase KP34p57 originated from phage KP34 marked as depo, at concentrations corresponding to 1000 MHF (minimal halo forming concentration), 100 MHF, 10 MHF, and 1 MHF, green bars; ciprofloxacin marked as cip, at concentrations corresponding to 4 MIC (minimal inhibitory concentration), 2 MIC, 1 MIC, and 0.5 MIC, yellow bars; and lytic phage KP34 marked as KP34 (10 9 PFU/ml and 10 6 PFU/ml, violet bars). Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P -value
    Figure Legend Snippet: The influence of depolymerase, ciprofloxacin, and infective phage KP34 on K. pneumoniae 77 biofilm formed for 72 h on polystyrene pegs. Three - day biofilm was treated for 2 h with: recombinant depolymerase KP34p57 originated from phage KP34 marked as depo, at concentrations corresponding to 1000 MHF (minimal halo forming concentration), 100 MHF, 10 MHF, and 1 MHF, green bars; ciprofloxacin marked as cip, at concentrations corresponding to 4 MIC (minimal inhibitory concentration), 2 MIC, 1 MIC, and 0.5 MIC, yellow bars; and lytic phage KP34 marked as KP34 (10 9 PFU/ml and 10 6 PFU/ml, violet bars). Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P -value

    Techniques Used: Recombinant, Concentration Assay, Fluorescence, Staining

    The antibiofilm activity of combined preparations composed of KP34p57 recombinant depolymerase (depo) at concentration corresponding to 10 MHF (minimal halo forming concentration), ciprofloxacin (cip) at concentration corresponding to 4 MIC (minimal inhibitory concentration), lytic phage KP34 specific to K. pneumoniae 77 (KP34, 10 8 PFU/ml), and lytic phage KP15 non-specific to K. pneumoniae 77 (KP15, 10 8 PFU/ml) on K. pneumoniae 77 biofilm formed for 72 h on polystyrene pegs. The 72 h biofilm was treated for two hours with different combinations of antimicrobials as indicated under the columns. Plus ( +) between abrreviations indicates that two or three antimicrobials were mixed and used simultaneously. Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P -value
    Figure Legend Snippet: The antibiofilm activity of combined preparations composed of KP34p57 recombinant depolymerase (depo) at concentration corresponding to 10 MHF (minimal halo forming concentration), ciprofloxacin (cip) at concentration corresponding to 4 MIC (minimal inhibitory concentration), lytic phage KP34 specific to K. pneumoniae 77 (KP34, 10 8 PFU/ml), and lytic phage KP15 non-specific to K. pneumoniae 77 (KP15, 10 8 PFU/ml) on K. pneumoniae 77 biofilm formed for 72 h on polystyrene pegs. The 72 h biofilm was treated for two hours with different combinations of antimicrobials as indicated under the columns. Plus ( +) between abrreviations indicates that two or three antimicrobials were mixed and used simultaneously. Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P -value

    Techniques Used: Activity Assay, Recombinant, Concentration Assay, Fluorescence, Staining

    The influence of depolymerase, ciprofloxacin, and infective phage KP34 on K. pneumoniae 77 biofilm formed for 24 h on polystyrene pegs. One-day biofilm was treated for 2 h with: recombinant depolymerase KP34p57 originated from phage KP34 marked as depo, at concentrations corresponding to 1000 MHF (minimal halo forming concentration), 100 MHF, 10 MHF, and 1 MHF, green bars; ciprofloxacin marked as cip, at concentrations corresponding to 4 MIC (minimal inhibitory concentration), 2 MIC, 1 MIC, and 0.5 MIC, yellow bars; and lytic phage KP34 marked as KP34 (10 9 PFU/ml and 10 6 PFU/ml, violet bars). Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P- value
    Figure Legend Snippet: The influence of depolymerase, ciprofloxacin, and infective phage KP34 on K. pneumoniae 77 biofilm formed for 24 h on polystyrene pegs. One-day biofilm was treated for 2 h with: recombinant depolymerase KP34p57 originated from phage KP34 marked as depo, at concentrations corresponding to 1000 MHF (minimal halo forming concentration), 100 MHF, 10 MHF, and 1 MHF, green bars; ciprofloxacin marked as cip, at concentrations corresponding to 4 MIC (minimal inhibitory concentration), 2 MIC, 1 MIC, and 0.5 MIC, yellow bars; and lytic phage KP34 marked as KP34 (10 9 PFU/ml and 10 6 PFU/ml, violet bars). Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P- value

    Techniques Used: Recombinant, Concentration Assay, Fluorescence, Staining

    The advantages and limitations of different biofilm monitoring assays used in this study: the colony count (left), LIVE/DEAD BacLight Bacterial Viability Kit (middle), biomass staining with crystal violet (right). The antibiofilm strategies were tested for: ( a ) untreated biofilm; ( b ) antibiotic application; ( c ) phage infection at a high and low MOI; ( d ) depolymerase action. The colony count indicates only culturable bacteria, thus the antibiotic and phage application showed the best antibiofilm effect. The life/dead ratio gave inconsistent results for ciprofloxacin treatment because of the specific antibiotic mode of action causing the elongation of cells and dyes accumulation. The CV staining is based on the dye interactions with the negative charge thus showed a false enlargement of biofilm after antibiotic, depolymerase, and phage at a low MOI application.
    Figure Legend Snippet: The advantages and limitations of different biofilm monitoring assays used in this study: the colony count (left), LIVE/DEAD BacLight Bacterial Viability Kit (middle), biomass staining with crystal violet (right). The antibiofilm strategies were tested for: ( a ) untreated biofilm; ( b ) antibiotic application; ( c ) phage infection at a high and low MOI; ( d ) depolymerase action. The colony count indicates only culturable bacteria, thus the antibiotic and phage application showed the best antibiofilm effect. The life/dead ratio gave inconsistent results for ciprofloxacin treatment because of the specific antibiotic mode of action causing the elongation of cells and dyes accumulation. The CV staining is based on the dye interactions with the negative charge thus showed a false enlargement of biofilm after antibiotic, depolymerase, and phage at a low MOI application.

    Techniques Used: Staining, Infection

    The influence of depolymerase, ciprofloxacin, and infective phage KP34 on K. pneumoniae 77 biofilm formed for 48 h on polystyrene pegs. Two-day biofilm was treated for 2 h with: recombinant depolymerase KP34p57 originated from phage KP34 marked as depo, at concentrations corresponding to 1000 MHF (minimal halo forming concentration), 100 MHF, 10 MHF, and 1 MHF, green bars; ciprofloxacin marked as cip, at concentrations corresponding to 4 MIC (minimal inhibitory concentration), 2 MIC, 1 MIC, and 0.5 MIC, yellow bars; and lytic phage KP34 marked as KP34 (10 9 PFU/ml and 10 6 PFU/ml, violet bars). Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P -value
    Figure Legend Snippet: The influence of depolymerase, ciprofloxacin, and infective phage KP34 on K. pneumoniae 77 biofilm formed for 48 h on polystyrene pegs. Two-day biofilm was treated for 2 h with: recombinant depolymerase KP34p57 originated from phage KP34 marked as depo, at concentrations corresponding to 1000 MHF (minimal halo forming concentration), 100 MHF, 10 MHF, and 1 MHF, green bars; ciprofloxacin marked as cip, at concentrations corresponding to 4 MIC (minimal inhibitory concentration), 2 MIC, 1 MIC, and 0.5 MIC, yellow bars; and lytic phage KP34 marked as KP34 (10 9 PFU/ml and 10 6 PFU/ml, violet bars). Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P -value

    Techniques Used: Recombinant, Concentration Assay, Fluorescence, Staining

    Related Articles

    Incubation:

    Article Title: Detection of Prosecretory Mitogen Lacritin in Nonprimate Tears Primarily as a C-Terminal-Like Fragment
    Article Snippet: .. Wells were washed and blocked with PBS-T and then incubated for 1 hour at 37°C with anti-N- or -C-terminal anti-lacritin antibody (1:200 in PBS-T), washed with PBS-T, incubated for 1 hour at 37°C with goat anti-rabbit IgG (1:1000; MP Biomedicals, Solon, OH), washed with PBS-T, and finally treated with o-phenylenediamine (OPD) substrate (Acros Organics, Geel, Belgium) for 10 minutes, followed by measurement at OD 415 in a Bio-Rad 680 ELISA plate reader. ..

    Article Title: Tear Lacritin Levels by Age, Sex, and Time of Day in Healthy Adults
    Article Snippet: Recombinant lacritin, N-65, or tear samples were loaded on 4% to 20% Mini-PROTEAN TGX precast gels (Bio-Rad), electrophoresed at 200 V, and transferred to nitrocellulose (Protran BA 83; Whatman, Dassel, Germany). .. Blots were blocked with PBS-T, incubated with anti-Lac Pep N-Term (1:500 dilution in PBS-T) for 2 hours at room temperature, rinsed with PBS-T, and incubated for 2 hours at room temperature with HRP-conjugated goat anti-rabbit IgG (MP Biomedicals) diluted 1:10,000 in PBS-T. Blots were rinsed with PBS-T and developed via chemiluminescence with Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific Inc., Rockford, IL). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Detection of Prosecretory Mitogen Lacritin in Nonprimate Tears Primarily as a C-Terminal-Like Fragment
    Article Snippet: .. Wells were washed and blocked with PBS-T and then incubated for 1 hour at 37°C with anti-N- or -C-terminal anti-lacritin antibody (1:200 in PBS-T), washed with PBS-T, incubated for 1 hour at 37°C with goat anti-rabbit IgG (1:1000; MP Biomedicals, Solon, OH), washed with PBS-T, and finally treated with o-phenylenediamine (OPD) substrate (Acros Organics, Geel, Belgium) for 10 minutes, followed by measurement at OD 415 in a Bio-Rad 680 ELISA plate reader. ..

    other:

    Article Title: Advantages and limitations of microtiter biofilm assays in the model of antibiofilm activity of Klebsiella phage KP34 and its depolymerase
    Article Snippet: The lid with pegs was transferred to fresh TSB broth every 24 h. After biofilm formation, pegs were washed in saline and then transferred to fresh TSB containing different concentrations of depolymerase KP34p57 (1000 MHF, 100 MHF, 10 MHF, and 1 MHF), ciprofloxacin (MP Biomedicals, Thermo Fisher Scientific Waltham, Massachusetts, USA, 4 MIC, 2 MIC, 1 MIC, and 0.5 MIC) and phage KP34 (106 and 109 PFU/ml).

    Article Title: Trim37-deficient mice recapitulate several features of the multi-organ disorder Mulibrey nanism
    Article Snippet: Liver pieces (20–50 mg) were homogenized by use of Thermo Savant FastPrep FP120 instrument and MP Lysing Matrix D tubes (MP Biomedicals, Santa Ana, CA) in 2:1 chloroform:methanol (v/v).

    Article Title: DNA replication and spindle checkpoints cooperate during S phase to delay mitosis and preserve genome integrity
    Article Snippet: Cells were then lysed using a FastPrep 120 (Thermo Savant; MP Biomedicals).

    Mass Spectrometry:

    Article Title: Effects of ultrasound therapy on the synovial fluid proteome in a rabbit surgery-induced model of knee osteoarthritis
    Article Snippet: NZW rabbits were provided by the Fujian University of Traditional Chinese Medicine animal testing center, batch number: SCXK (Shanghai) 2012-0011. .. Easy-nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiscan FC Microplate Photometer (Thermo Scientific), Centrifuges (Eppendorf 5430R), Concentrator plus/Vacufuge (Eppendorf Concentrator Plus) Electrophoresis apparatus (GE Healthcare EPS601), Vertical Electrophoresis Tanks (SE260; GE-Healthcare), Thermofinnigan Easy-nLC 1000, Trap column, ASY column SC001 traps (RP-C18 ), Analysis column, EASY column SC200 (RP-C18), Maxquant (version 1.3.0.5), Perseus (version 1.3.0.4), MP Fastprep-24 Homogenate instrument (MP Biomedicals), Ultrasonic Cell Disrupter System, Constant temperature incubator, Vortex oscillator, ProteomeDiscoverer 1.4 (Thermo Scientific), MASCOT 2.2 (Matrix Science), Perseus 1.3 (M & M), 10 kDa Ultrafiltration centrifuge tubes, C18 Cartridge, Multiple Affinity Removal LC Column—Human 14/Mouse 3, iTRAQ Reagent‐4/8plex Multiplex Kit, Dissolution buffer (AB SCIEX), SCX chromatographic column, Polysulfoethyl (PolyLCInc, Maryland, U.S.A.). ..

    Electrophoresis:

    Article Title: Effects of ultrasound therapy on the synovial fluid proteome in a rabbit surgery-induced model of knee osteoarthritis
    Article Snippet: NZW rabbits were provided by the Fujian University of Traditional Chinese Medicine animal testing center, batch number: SCXK (Shanghai) 2012-0011. .. Easy-nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiscan FC Microplate Photometer (Thermo Scientific), Centrifuges (Eppendorf 5430R), Concentrator plus/Vacufuge (Eppendorf Concentrator Plus) Electrophoresis apparatus (GE Healthcare EPS601), Vertical Electrophoresis Tanks (SE260; GE-Healthcare), Thermofinnigan Easy-nLC 1000, Trap column, ASY column SC001 traps (RP-C18 ), Analysis column, EASY column SC200 (RP-C18), Maxquant (version 1.3.0.5), Perseus (version 1.3.0.4), MP Fastprep-24 Homogenate instrument (MP Biomedicals), Ultrasonic Cell Disrupter System, Constant temperature incubator, Vortex oscillator, ProteomeDiscoverer 1.4 (Thermo Scientific), MASCOT 2.2 (Matrix Science), Perseus 1.3 (M & M), 10 kDa Ultrafiltration centrifuge tubes, C18 Cartridge, Multiple Affinity Removal LC Column—Human 14/Mouse 3, iTRAQ Reagent‐4/8plex Multiplex Kit, Dissolution buffer (AB SCIEX), SCX chromatographic column, Polysulfoethyl (PolyLCInc, Maryland, U.S.A.). ..

    Multiplex Assay:

    Article Title: Effects of ultrasound therapy on the synovial fluid proteome in a rabbit surgery-induced model of knee osteoarthritis
    Article Snippet: NZW rabbits were provided by the Fujian University of Traditional Chinese Medicine animal testing center, batch number: SCXK (Shanghai) 2012-0011. .. Easy-nLC Liquid Chromatograph (Thermo Scientific), Q Exactive Mass Spectrometer (Thermo Scientific), AKTA Purifier 100 (GE Healthcare), Multiscan FC Microplate Photometer (Thermo Scientific), Centrifuges (Eppendorf 5430R), Concentrator plus/Vacufuge (Eppendorf Concentrator Plus) Electrophoresis apparatus (GE Healthcare EPS601), Vertical Electrophoresis Tanks (SE260; GE-Healthcare), Thermofinnigan Easy-nLC 1000, Trap column, ASY column SC001 traps (RP-C18 ), Analysis column, EASY column SC200 (RP-C18), Maxquant (version 1.3.0.5), Perseus (version 1.3.0.4), MP Fastprep-24 Homogenate instrument (MP Biomedicals), Ultrasonic Cell Disrupter System, Constant temperature incubator, Vortex oscillator, ProteomeDiscoverer 1.4 (Thermo Scientific), MASCOT 2.2 (Matrix Science), Perseus 1.3 (M & M), 10 kDa Ultrafiltration centrifuge tubes, C18 Cartridge, Multiple Affinity Removal LC Column—Human 14/Mouse 3, iTRAQ Reagent‐4/8plex Multiplex Kit, Dissolution buffer (AB SCIEX), SCX chromatographic column, Polysulfoethyl (PolyLCInc, Maryland, U.S.A.). ..

    Fluorescence In Situ Hybridization:

    Article Title: MYCN is amplified during S phase, and c-myb is involved in controlling MYCN expression and amplification in MYCN-amplified neuroblastoma cell lines
    Article Snippet: Fluorescence in situ hybridization (FISH) FISH was performed as previously described ( , ). .. In FISH experiments, MYCN gene (2p24)/Chromosome 2 α-Satellite (red/green; cat. no. PONC0224; Qbiogene, Inc.; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 1p36/Chr 1 SE (Poseidon probe red/green, cat. no. KB-10705; Kreatech Diagnostics Corp., Amsterdam, LG, Netherlands) and 1p36/1q25 (Vysis probe orange/green, cat. no. 32-231004; Abbott Pharmaceutical Co. Ltd., Lake Bluff, IL, USA) probes were used. .. Neuroblastoma cells were seeded into 25-cm2 tissue culture flasks and grown in culture medium specific for each cell line (please see ‘Cell culture ’ section for the characteristics of each growth medium) in a humidified atmosphere containing 5% CO2 at 37°C.

    Western Blot:

    Article Title: Tear Lacritin Levels by Age, Sex, and Time of Day in Healthy Adults
    Article Snippet: Recombinant lacritin, N-65, or tear samples were loaded on 4% to 20% Mini-PROTEAN TGX precast gels (Bio-Rad), electrophoresed at 200 V, and transferred to nitrocellulose (Protran BA 83; Whatman, Dassel, Germany). .. Blots were blocked with PBS-T, incubated with anti-Lac Pep N-Term (1:500 dilution in PBS-T) for 2 hours at room temperature, rinsed with PBS-T, and incubated for 2 hours at room temperature with HRP-conjugated goat anti-rabbit IgG (MP Biomedicals) diluted 1:10,000 in PBS-T. Blots were rinsed with PBS-T and developed via chemiluminescence with Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific Inc., Rockford, IL). ..

    Concentration Assay:

    Article Title: Advantages and limitations of microtiter biofilm assays in the model of antibiofilm activity of Klebsiella phage KP34 and its depolymerase
    Article Snippet: Figure a depicts a spot test on K. pneumoniae 77 bacterial lawn and S2b K. pneumoniae ATCC 700603 bacterial lawn. .. Minimal inhibitory concentration (MIC) determination of ciprofloxacin The MIC of ciprofloxacin (MP Biomedicals, Thermo Fisher Scientific Waltham, Massachusetts, USA) was determined by the broth dilution method according to CLSI (Clinical Laboratory Standards Institute) recommendations. .. Klebsiella pneumoniae 77 grown on Mueller Hinton Agar (MHA, bioMerieux, Marcy l'Etoile, France) was resuspended in saline to 0.5 McFarland standard (approximately 1.5 × 108 CFU/ml) using a densitometer (bioMerieux, Marcy l'Etoile, France).

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  • 94
    Valiant mycn gene
    <t>MYCN</t> amplification and 1p36 alterations from interphase and metaphase in neuroblastoma cell lines. (A) Neuroblastoma cells were harvested for <t>FISH</t> experiments (magnification, ×40). (B) MYCN amplification status of interphase nuclei was determined by FISH. (C) Modal number, hsr and dmin from metaphase in neuroblastoma cell lines are demonstrated. (D) 1p36 alterations from metaphase and interphase in neuroblastoma cell lines are presented. Magnification for B-D, ×100. MYCN, MYCN proto-oncogene bHLH transcription factor ; ACE3, amplification-control-element-on-3 ; FISH, fluorescence in situ hybridization; del, deletion; dmin, double minute; hsr, homogeneously staining region; imb, imbalance; SE, satellite enumeration.
    Mycn Gene, supplied by Valiant, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mycn gene/product/Valiant
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mycn gene - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

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    MYCN amplification and 1p36 alterations from interphase and metaphase in neuroblastoma cell lines. (A) Neuroblastoma cells were harvested for FISH experiments (magnification, ×40). (B) MYCN amplification status of interphase nuclei was determined by FISH. (C) Modal number, hsr and dmin from metaphase in neuroblastoma cell lines are demonstrated. (D) 1p36 alterations from metaphase and interphase in neuroblastoma cell lines are presented. Magnification for B-D, ×100. MYCN, MYCN proto-oncogene bHLH transcription factor ; ACE3, amplification-control-element-on-3 ; FISH, fluorescence in situ hybridization; del, deletion; dmin, double minute; hsr, homogeneously staining region; imb, imbalance; SE, satellite enumeration.

    Journal: Molecular Medicine Reports

    Article Title: MYCN is amplified during S phase, and c-myb is involved in controlling MYCN expression and amplification in MYCN-amplified neuroblastoma cell lines

    doi: 10.3892/mmr.2018.9686

    Figure Lengend Snippet: MYCN amplification and 1p36 alterations from interphase and metaphase in neuroblastoma cell lines. (A) Neuroblastoma cells were harvested for FISH experiments (magnification, ×40). (B) MYCN amplification status of interphase nuclei was determined by FISH. (C) Modal number, hsr and dmin from metaphase in neuroblastoma cell lines are demonstrated. (D) 1p36 alterations from metaphase and interphase in neuroblastoma cell lines are presented. Magnification for B-D, ×100. MYCN, MYCN proto-oncogene bHLH transcription factor ; ACE3, amplification-control-element-on-3 ; FISH, fluorescence in situ hybridization; del, deletion; dmin, double minute; hsr, homogeneously staining region; imb, imbalance; SE, satellite enumeration.

    Article Snippet: In FISH experiments, MYCN gene (2p24)/Chromosome 2 α-Satellite (red/green; cat. no. PONC0224; Qbiogene, Inc.; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 1p36/Chr 1 SE (Poseidon probe red/green, cat. no. KB-10705; Kreatech Diagnostics Corp., Amsterdam, LG, Netherlands) and 1p36/1q25 (Vysis probe orange/green, cat. no. 32-231004; Abbott Pharmaceutical Co. Ltd., Lake Bluff, IL, USA) probes were used.

    Techniques: Amplification, Fluorescence In Situ Hybridization, Fluorescence, In Situ Hybridization, Staining

    The influence of depolymerase, ciprofloxacin, and infective phage KP34 on K. pneumoniae 77 biofilm formed for 72 h on polystyrene pegs. Three - day biofilm was treated for 2 h with: recombinant depolymerase KP34p57 originated from phage KP34 marked as depo, at concentrations corresponding to 1000 MHF (minimal halo forming concentration), 100 MHF, 10 MHF, and 1 MHF, green bars; ciprofloxacin marked as cip, at concentrations corresponding to 4 MIC (minimal inhibitory concentration), 2 MIC, 1 MIC, and 0.5 MIC, yellow bars; and lytic phage KP34 marked as KP34 (10 9 PFU/ml and 10 6 PFU/ml, violet bars). Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P -value

    Journal: Scientific Reports

    Article Title: Advantages and limitations of microtiter biofilm assays in the model of antibiofilm activity of Klebsiella phage KP34 and its depolymerase

    doi: 10.1038/s41598-020-77198-5

    Figure Lengend Snippet: The influence of depolymerase, ciprofloxacin, and infective phage KP34 on K. pneumoniae 77 biofilm formed for 72 h on polystyrene pegs. Three - day biofilm was treated for 2 h with: recombinant depolymerase KP34p57 originated from phage KP34 marked as depo, at concentrations corresponding to 1000 MHF (minimal halo forming concentration), 100 MHF, 10 MHF, and 1 MHF, green bars; ciprofloxacin marked as cip, at concentrations corresponding to 4 MIC (minimal inhibitory concentration), 2 MIC, 1 MIC, and 0.5 MIC, yellow bars; and lytic phage KP34 marked as KP34 (10 9 PFU/ml and 10 6 PFU/ml, violet bars). Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P -value

    Article Snippet: The lid with pegs was transferred to fresh TSB broth every 24 h. After biofilm formation, pegs were washed in saline and then transferred to fresh TSB containing different concentrations of depolymerase KP34p57 (1000 MHF, 100 MHF, 10 MHF, and 1 MHF), ciprofloxacin (MP Biomedicals, Thermo Fisher Scientific Waltham, Massachusetts, USA, 4 MIC, 2 MIC, 1 MIC, and 0.5 MIC) and phage KP34 (106 and 109 PFU/ml).

    Techniques: Recombinant, Concentration Assay, Fluorescence, Staining

    The antibiofilm activity of combined preparations composed of KP34p57 recombinant depolymerase (depo) at concentration corresponding to 10 MHF (minimal halo forming concentration), ciprofloxacin (cip) at concentration corresponding to 4 MIC (minimal inhibitory concentration), lytic phage KP34 specific to K. pneumoniae 77 (KP34, 10 8 PFU/ml), and lytic phage KP15 non-specific to K. pneumoniae 77 (KP15, 10 8 PFU/ml) on K. pneumoniae 77 biofilm formed for 72 h on polystyrene pegs. The 72 h biofilm was treated for two hours with different combinations of antimicrobials as indicated under the columns. Plus ( +) between abrreviations indicates that two or three antimicrobials were mixed and used simultaneously. Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P -value

    Journal: Scientific Reports

    Article Title: Advantages and limitations of microtiter biofilm assays in the model of antibiofilm activity of Klebsiella phage KP34 and its depolymerase

    doi: 10.1038/s41598-020-77198-5

    Figure Lengend Snippet: The antibiofilm activity of combined preparations composed of KP34p57 recombinant depolymerase (depo) at concentration corresponding to 10 MHF (minimal halo forming concentration), ciprofloxacin (cip) at concentration corresponding to 4 MIC (minimal inhibitory concentration), lytic phage KP34 specific to K. pneumoniae 77 (KP34, 10 8 PFU/ml), and lytic phage KP15 non-specific to K. pneumoniae 77 (KP15, 10 8 PFU/ml) on K. pneumoniae 77 biofilm formed for 72 h on polystyrene pegs. The 72 h biofilm was treated for two hours with different combinations of antimicrobials as indicated under the columns. Plus ( +) between abrreviations indicates that two or three antimicrobials were mixed and used simultaneously. Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P -value

    Article Snippet: The lid with pegs was transferred to fresh TSB broth every 24 h. After biofilm formation, pegs were washed in saline and then transferred to fresh TSB containing different concentrations of depolymerase KP34p57 (1000 MHF, 100 MHF, 10 MHF, and 1 MHF), ciprofloxacin (MP Biomedicals, Thermo Fisher Scientific Waltham, Massachusetts, USA, 4 MIC, 2 MIC, 1 MIC, and 0.5 MIC) and phage KP34 (106 and 109 PFU/ml).

    Techniques: Activity Assay, Recombinant, Concentration Assay, Fluorescence, Staining

    The influence of depolymerase, ciprofloxacin, and infective phage KP34 on K. pneumoniae 77 biofilm formed for 24 h on polystyrene pegs. One-day biofilm was treated for 2 h with: recombinant depolymerase KP34p57 originated from phage KP34 marked as depo, at concentrations corresponding to 1000 MHF (minimal halo forming concentration), 100 MHF, 10 MHF, and 1 MHF, green bars; ciprofloxacin marked as cip, at concentrations corresponding to 4 MIC (minimal inhibitory concentration), 2 MIC, 1 MIC, and 0.5 MIC, yellow bars; and lytic phage KP34 marked as KP34 (10 9 PFU/ml and 10 6 PFU/ml, violet bars). Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P- value

    Journal: Scientific Reports

    Article Title: Advantages and limitations of microtiter biofilm assays in the model of antibiofilm activity of Klebsiella phage KP34 and its depolymerase

    doi: 10.1038/s41598-020-77198-5

    Figure Lengend Snippet: The influence of depolymerase, ciprofloxacin, and infective phage KP34 on K. pneumoniae 77 biofilm formed for 24 h on polystyrene pegs. One-day biofilm was treated for 2 h with: recombinant depolymerase KP34p57 originated from phage KP34 marked as depo, at concentrations corresponding to 1000 MHF (minimal halo forming concentration), 100 MHF, 10 MHF, and 1 MHF, green bars; ciprofloxacin marked as cip, at concentrations corresponding to 4 MIC (minimal inhibitory concentration), 2 MIC, 1 MIC, and 0.5 MIC, yellow bars; and lytic phage KP34 marked as KP34 (10 9 PFU/ml and 10 6 PFU/ml, violet bars). Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P- value

    Article Snippet: The lid with pegs was transferred to fresh TSB broth every 24 h. After biofilm formation, pegs were washed in saline and then transferred to fresh TSB containing different concentrations of depolymerase KP34p57 (1000 MHF, 100 MHF, 10 MHF, and 1 MHF), ciprofloxacin (MP Biomedicals, Thermo Fisher Scientific Waltham, Massachusetts, USA, 4 MIC, 2 MIC, 1 MIC, and 0.5 MIC) and phage KP34 (106 and 109 PFU/ml).

    Techniques: Recombinant, Concentration Assay, Fluorescence, Staining

    The advantages and limitations of different biofilm monitoring assays used in this study: the colony count (left), LIVE/DEAD BacLight Bacterial Viability Kit (middle), biomass staining with crystal violet (right). The antibiofilm strategies were tested for: ( a ) untreated biofilm; ( b ) antibiotic application; ( c ) phage infection at a high and low MOI; ( d ) depolymerase action. The colony count indicates only culturable bacteria, thus the antibiotic and phage application showed the best antibiofilm effect. The life/dead ratio gave inconsistent results for ciprofloxacin treatment because of the specific antibiotic mode of action causing the elongation of cells and dyes accumulation. The CV staining is based on the dye interactions with the negative charge thus showed a false enlargement of biofilm after antibiotic, depolymerase, and phage at a low MOI application.

    Journal: Scientific Reports

    Article Title: Advantages and limitations of microtiter biofilm assays in the model of antibiofilm activity of Klebsiella phage KP34 and its depolymerase

    doi: 10.1038/s41598-020-77198-5

    Figure Lengend Snippet: The advantages and limitations of different biofilm monitoring assays used in this study: the colony count (left), LIVE/DEAD BacLight Bacterial Viability Kit (middle), biomass staining with crystal violet (right). The antibiofilm strategies were tested for: ( a ) untreated biofilm; ( b ) antibiotic application; ( c ) phage infection at a high and low MOI; ( d ) depolymerase action. The colony count indicates only culturable bacteria, thus the antibiotic and phage application showed the best antibiofilm effect. The life/dead ratio gave inconsistent results for ciprofloxacin treatment because of the specific antibiotic mode of action causing the elongation of cells and dyes accumulation. The CV staining is based on the dye interactions with the negative charge thus showed a false enlargement of biofilm after antibiotic, depolymerase, and phage at a low MOI application.

    Article Snippet: The lid with pegs was transferred to fresh TSB broth every 24 h. After biofilm formation, pegs were washed in saline and then transferred to fresh TSB containing different concentrations of depolymerase KP34p57 (1000 MHF, 100 MHF, 10 MHF, and 1 MHF), ciprofloxacin (MP Biomedicals, Thermo Fisher Scientific Waltham, Massachusetts, USA, 4 MIC, 2 MIC, 1 MIC, and 0.5 MIC) and phage KP34 (106 and 109 PFU/ml).

    Techniques: Staining, Infection

    The influence of depolymerase, ciprofloxacin, and infective phage KP34 on K. pneumoniae 77 biofilm formed for 48 h on polystyrene pegs. Two-day biofilm was treated for 2 h with: recombinant depolymerase KP34p57 originated from phage KP34 marked as depo, at concentrations corresponding to 1000 MHF (minimal halo forming concentration), 100 MHF, 10 MHF, and 1 MHF, green bars; ciprofloxacin marked as cip, at concentrations corresponding to 4 MIC (minimal inhibitory concentration), 2 MIC, 1 MIC, and 0.5 MIC, yellow bars; and lytic phage KP34 marked as KP34 (10 9 PFU/ml and 10 6 PFU/ml, violet bars). Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P -value

    Journal: Scientific Reports

    Article Title: Advantages and limitations of microtiter biofilm assays in the model of antibiofilm activity of Klebsiella phage KP34 and its depolymerase

    doi: 10.1038/s41598-020-77198-5

    Figure Lengend Snippet: The influence of depolymerase, ciprofloxacin, and infective phage KP34 on K. pneumoniae 77 biofilm formed for 48 h on polystyrene pegs. Two-day biofilm was treated for 2 h with: recombinant depolymerase KP34p57 originated from phage KP34 marked as depo, at concentrations corresponding to 1000 MHF (minimal halo forming concentration), 100 MHF, 10 MHF, and 1 MHF, green bars; ciprofloxacin marked as cip, at concentrations corresponding to 4 MIC (minimal inhibitory concentration), 2 MIC, 1 MIC, and 0.5 MIC, yellow bars; and lytic phage KP34 marked as KP34 (10 9 PFU/ml and 10 6 PFU/ml, violet bars). Biofilm eradication was examined with three different microtiter methods: ( a ) the colony count (CFU/ml); ( b ) LIVE/DEAD BacLight Bacterial Viability Kit with live to dead cells ratio at fluorescence 530/630 nm; ( c ) the crystal violet staining and the level of absorbance at 595 nm. P -value

    Article Snippet: The lid with pegs was transferred to fresh TSB broth every 24 h. After biofilm formation, pegs were washed in saline and then transferred to fresh TSB containing different concentrations of depolymerase KP34p57 (1000 MHF, 100 MHF, 10 MHF, and 1 MHF), ciprofloxacin (MP Biomedicals, Thermo Fisher Scientific Waltham, Massachusetts, USA, 4 MIC, 2 MIC, 1 MIC, and 0.5 MIC) and phage KP34 (106 and 109 PFU/ml).

    Techniques: Recombinant, Concentration Assay, Fluorescence, Staining