rabbit polyclonal igg  (Valiant)

 
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    Name:
    Anti synaptophysin rabbit polyclonal antibody
    Description:
    Synaptophysin is a calcium binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors
    Catalog Number:
    0810706
    Price:
    698.25
    Category:
    Life Sciences Antibodies Primary Antibodies
    Applications:
    Immunohistochemistry ,Immunoblot
    Size:
    250 µL
    Buy from Supplier


    Structured Review

    Valiant rabbit polyclonal igg
    Synaptophysin is a calcium binding and integral membrane glycoprotein present in presynaptic vesicles in almost all neurons Synaptophysin has four transmembrane domains and it forms a complex with dynamin at high calcium concentrations suggesting an involvement in synaptic vesicle endocytosis Synaptophysin is currently the most widely used marker for nerve terminals and for differentiating neuroendocrine tumors
    https://www.bioz.com/result/rabbit polyclonal igg/product/Valiant
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal igg - by Bioz Stars, 2021-04
    92/100 stars

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    Related Articles

    Incubation:

    Article Title: A role of stochastic phenotype switching in generating mosaic endothelial cell heterogeneity
    Article Snippet: Immunofluorescent co-staining of en face aorta Aortas were fixed in 4% paraformaldehyde (PFA), washed in PBS, and blocked with TNB Blocking Buffer (TNBT) (0.1 M Tris-HCl, 0.15 M NaCl, 0.2% triton-X-100, 0.5% blocking, Perlin Elmer). .. Aortas were incubated in Image-iT FX signal enhancer (Invitrogen) at room temperature for 30 min and assayed using primary antibodies (rabbit monoclonal vWF (Dako Cat. No. A0082) diluted at 1:1,000, Armenian hamster polyclonal CD31 (Millipore Cat. No. MAB1398Z) diluted at 1:100, and rabbit polyclonal β-galactosidase (MP Biomedicals Cat. No. 08559761) diluted at 1:50) diluted in TNBT at 4 °C overnight. .. Next, aortas were washed with PBS and incubated with secondary antibodies (Alexa Fluor 594 (Invitrogen) and Alexa Fluor 488 (Jackson ImmunoResearch) diluted at 1:200) for 2 h at room temperature.

    Article Title: Neuromuscular Adaptability of Male and Female Rats to Muscle Unloading
    Article Snippet: The next day, sections were washed 4 × 15 min in PBS with 1% BSA before being incubated for 2 h at room temperature in Alexafluor 488 conjugated secondary immunoglobulin (Molecular Probes, Eugene, OR, Cat#A11001; RRID; AB_2534069) which had been diluted 1:200 in PBS with 1% BSA. .. Sections were then washed 4 × 15 min in PBS with 1% BSA before being incubated in a humidified chamber overnight at 4°C in a solution containing rhodamine conjugated α-bungarotoxin (BTX; Molecular Probes, Eugene, OR, Cat# T1175) diluted 1:600 in PBS, along with anti-synaptophysin (MP Biomedicals, Solon, OH, Cat0810706, RRID; AB_2334188) at a dilution of 1:50. ..

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Neuromuscular Adaptability of Male and Female Rats to Muscle Unloading
    Article Snippet: The next day, sections were washed 4 × 15 min in PBS with 1% BSA before being incubated for 2 h at room temperature in Alexafluor 488 conjugated secondary immunoglobulin (Molecular Probes, Eugene, OR, Cat#A11001; RRID; AB_2534069) which had been diluted 1:200 in PBS with 1% BSA. .. Sections were then washed 4 × 15 min in PBS with 1% BSA before being incubated in a humidified chamber overnight at 4°C in a solution containing rhodamine conjugated α-bungarotoxin (BTX; Molecular Probes, Eugene, OR, Cat# T1175) diluted 1:600 in PBS, along with anti-synaptophysin (MP Biomedicals, Solon, OH, Cat0810706, RRID; AB_2334188) at a dilution of 1:50. ..

    Modification:

    Article Title: Activation of Murine Macrophages
    Article Snippet: .. Additional Materials (also see Basic Protocol) OVA: 1 mg/ml chicken egg albumin (OVA; Worthington Biochemical, cat. no. LS003049) in Dulbecco’s modified Eagle medium/F12 (DMEM/F12; GIBCO) Detoxi-gel endotoxin removing column (Pierce, cat. no. 20344) Anti-OVA IgG: 4 mg/ml of rabbit polyclonal IgG to chicken egg albumin (Cappel anti-OVA IgG; MP Biomedicals, cat. no. 55304/05490) in DMEM/F12 Endotoxin removal buffer for protein solution (EndoClean; BioVintage, cat. no. 18602) Mini-rotator (Glas-Col), or equivalent Additional reagents and equipment for performing ELISA ( unit 2.1 ) Remove endotoxin from OVA with a Detoxi-Gel endotoxin removing column, following manufacturer’s instructions. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Activation of Murine Macrophages
    Article Snippet: .. Additional Materials (also see Basic Protocol) OVA: 1 mg/ml chicken egg albumin (OVA; Worthington Biochemical, cat. no. LS003049) in Dulbecco’s modified Eagle medium/F12 (DMEM/F12; GIBCO) Detoxi-gel endotoxin removing column (Pierce, cat. no. 20344) Anti-OVA IgG: 4 mg/ml of rabbit polyclonal IgG to chicken egg albumin (Cappel anti-OVA IgG; MP Biomedicals, cat. no. 55304/05490) in DMEM/F12 Endotoxin removal buffer for protein solution (EndoClean; BioVintage, cat. no. 18602) Mini-rotator (Glas-Col), or equivalent Additional reagents and equipment for performing ELISA ( unit 2.1 ) Remove endotoxin from OVA with a Detoxi-Gel endotoxin removing column, following manufacturer’s instructions. ..

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  • 92
    Valiant rabbit polyclonal igg
    Rabbit Polyclonal Igg, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal igg/product/Valiant
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal igg - by Bioz Stars, 2021-04
    92/100 stars
      Buy from Supplier

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