dss  (Valiant)

 
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  • 95
    Name:
    Dextran
    Description:
    Dextran
    Catalog Number:
    0520519580
    Price:
    95.7
    Category:
    Life Sciences Chemicals Organics
    Applications:
    Plasma extender
    Size:
    100 g
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    Structured Review

    Valiant dss
    Dextran
    Dextran
    https://www.bioz.com/result/dss/product/Valiant
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dss - by Bioz Stars, 2021-04
    95/100 stars

    Images

    1) Product Images from "Peptidoglycan recognition protein 3 and Nod2 synergistically protect mice from dextran sodium sulfate-induced colitis"

    Article Title: Peptidoglycan recognition protein 3 and Nod2 synergistically protect mice from dextran sodium sulfate-induced colitis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1301548

    Pglyrp3 −/− Nod2 −/− mice express higher levels of chemokine and cytokine mRNA in the colon following DSS treatment
    Figure Legend Snippet: Pglyrp3 −/− Nod2 −/− mice express higher levels of chemokine and cytokine mRNA in the colon following DSS treatment

    Techniques Used: Mouse Assay

    Pglyrp3 −/− Nod2 −/− and Pglyrp3 −/− mice have higher levels of ATP in the colon, and intestinal ATP preferentially increases sensitivity of Nod2 −/− mice to DSS-colitis
    Figure Legend Snippet: Pglyrp3 −/− Nod2 −/− and Pglyrp3 −/− mice have higher levels of ATP in the colon, and intestinal ATP preferentially increases sensitivity of Nod2 −/− mice to DSS-colitis

    Techniques Used: Mouse Assay

    Pglyrp3 −/− Nod2 −/− mice are more susceptible to DSS-induced colitis than Pglyrp3 and Nod2 single-deficient mice
    Figure Legend Snippet: Pglyrp3 −/− Nod2 −/− mice are more susceptible to DSS-induced colitis than Pglyrp3 and Nod2 single-deficient mice

    Techniques Used: Mouse Assay

    Related Articles

    Filtration:

    Article Title: Cationic Polymers for the Delivery of the Ebola DNA Vaccine Encoding Artificial T-Cell Immunogen
    Article Snippet: .. Dextran 40,000 (MP Biomedicals ™, USA) was treated with sodium periodate (at the rate of 40 sodium periodate molecules per 1 dextran molecule) for 60 min in H2 O and then gel filtration was performed on a Sephadex G-25 column in 50 mM carbonate buffer (pH 8.6). .. Aldehyde functions formed were quantified by a colorimetric assay (546 nm) using 2,3,5-triphenyltetrazolium salts being converted into formazan.

    Mouse Assay:

    Article Title: Peptidoglycan recognition protein 3 and Nod2 synergistically protect mice from dextran sodium sulfate-induced colitis
    Article Snippet: The Indiana University School of Medicine–Northwest Institutional Animal Care and Use Committee approved all experiments with mice. .. Experimental colitis was induced in WT, Nod2 −/− , Pglyrp3 −/− , and Pglyrp3 −/− Nod2 −/− mice with 5% DSS (dextran sulfate sodium, MP Biomedical) in drinking water ( ). .. DSS-induced intestinal inflammation is a well-established animal model for colitis and its manifestations include bloody diarrhea, weight loss, shortening of the colon, mucosal ulceration, and epithelial dysplasia.

    Article Title: Effects of a small molecule R-spondin-1 substitute RS-246204 on a mouse intestinal organoid culture
    Article Snippet: After 5 days of TGF-β1 treatment, immunocytochemistry was performed to analyze the efficiency of the OEMT. .. Induction of DSS-induced colitis and administration of RS-246204Seven-week-old C57Bl/6 mice were given a 2.5% dextran sulfate sodium (DSS) (MP Biomedicals, Aurora, OH) treatment in drinking water for 5 days. ..

    Article Title: Distinct inactivated bacterial-based immune modulators vary in their therapeutic efficacies for treating disease based on the organ site of pathology
    Article Snippet: DSS/AOM model Mice were injected intraperitoneally on day 0 with azoxymethane (AOM; Sigma, Kawasaki, Kanagawa Prefecture, Japan), at 8 mg/kg body weight, 10 days after treatment started. .. After AOM injections, mice received 3 cycles of dextran sodium sulphate (DSS) (MP Biomedicals, Santa Ana, CA, USA) treatment spaced 3 weeks apart. .. In each cycle, DSS was administered in the drinking water ad libitum for 5 days at a final concentration of 2.5% weight/volume.

    Article Title: HVEM Signalling Promotes Colitis
    Article Snippet: .. DSS- induced experimental colitis Acute colitis was induced in age-matched C57BL/6, HVEM-/- , LIGHT-/- and RAG1-/- mice, by oral administration of Dextran sulfate sodium (DSS) (MP Biomedicals) at a concentration of 5% (w/v) in drinking water for 4 days. .. Age-matched C57BL/6, HVEM-/- and LIGHT-/- mice receiving normal drinking water served as controls.

    Article Title: Interleukin-7 enables antibody responses to bacterial polysaccharides by promoting B cell antigen receptor diversity
    Article Snippet: .. For dextran immunizations, 50 μg of α1-6 dextran (B512; MP Biomedicals, LLC, Solon, OH) or 50 μg of α1-3 dextran (B1355; A gift from Dr. A. Jeanes) dissolved in 100 μl DPBS were used to immunize mice i.p. For whole bacterial immunization, mice were injected i.p. with 3×108 heat-killed Escherichia coli strain W3110 pDC5, which expresses ViPS , or 108 heat-killed, paraformaldehyde-fixed Enterobacter cloacae strain MK7, which expresses α1-3 dextran. .. ViPS-specific IgM and IgG were measured by incubating 96-well microtiter plates (Nunc MultiSorp 467340; Thermo Fisher Scientific Nunc A/S, Roskilde, Denmark) with 2 μg/ml of ViPS in DPBS overnight at room temperature.

    Article Title: Kr?ppel-Like Factor 5 Protects Against Dextran Sulfate Sodium-Induced Colonic Injury by Promoting Epithelial Repair in Mice
    Article Snippet: For inhibitor studies, Caco-2 cells were pretreated for 10 min prior to addition of DSS with U0126 at 50 μM (Cell Signaling Technology; Beverly, MA) or Bay 11-7082 at 20μM (Calbiochem; San Diego, CA). .. Colitis was induced in 7- to 8-week old gender-matched WT and Klf5+/− mice by by addition of dextran sulfate sodium (DSS) (molecular weight, 35,000–50,000; MP Biomedicals, Solon, OH) to the drinking water at 3.5% (wt/vol) for 7 days. ..

    Concentration Assay:

    Article Title: HVEM Signalling Promotes Colitis
    Article Snippet: .. DSS- induced experimental colitis Acute colitis was induced in age-matched C57BL/6, HVEM-/- , LIGHT-/- and RAG1-/- mice, by oral administration of Dextran sulfate sodium (DSS) (MP Biomedicals) at a concentration of 5% (w/v) in drinking water for 4 days. .. Age-matched C57BL/6, HVEM-/- and LIGHT-/- mice receiving normal drinking water served as controls.

    Injection:

    Article Title: Interleukin-7 enables antibody responses to bacterial polysaccharides by promoting B cell antigen receptor diversity
    Article Snippet: .. For dextran immunizations, 50 μg of α1-6 dextran (B512; MP Biomedicals, LLC, Solon, OH) or 50 μg of α1-3 dextran (B1355; A gift from Dr. A. Jeanes) dissolved in 100 μl DPBS were used to immunize mice i.p. For whole bacterial immunization, mice were injected i.p. with 3×108 heat-killed Escherichia coli strain W3110 pDC5, which expresses ViPS , or 108 heat-killed, paraformaldehyde-fixed Enterobacter cloacae strain MK7, which expresses α1-3 dextran. .. ViPS-specific IgM and IgG were measured by incubating 96-well microtiter plates (Nunc MultiSorp 467340; Thermo Fisher Scientific Nunc A/S, Roskilde, Denmark) with 2 μg/ml of ViPS in DPBS overnight at room temperature.

    Molecular Weight:

    Article Title: Kr?ppel-Like Factor 5 Protects Against Dextran Sulfate Sodium-Induced Colonic Injury by Promoting Epithelial Repair in Mice
    Article Snippet: For inhibitor studies, Caco-2 cells were pretreated for 10 min prior to addition of DSS with U0126 at 50 μM (Cell Signaling Technology; Beverly, MA) or Bay 11-7082 at 20μM (Calbiochem; San Diego, CA). .. Colitis was induced in 7- to 8-week old gender-matched WT and Klf5+/− mice by by addition of dextran sulfate sodium (DSS) (molecular weight, 35,000–50,000; MP Biomedicals, Solon, OH) to the drinking water at 3.5% (wt/vol) for 7 days. ..

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  • dss  (Valiant)
    95
    Valiant dss
    <t>Pglyrp3</t> −/− Nod2 −/− mice express higher levels of chemokine and cytokine mRNA in the colon following <t>DSS</t> treatment
    Dss, supplied by Valiant, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dss/product/Valiant
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dss - by Bioz Stars, 2021-04
    95/100 stars
      Buy from Supplier

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    Pglyrp3 −/− Nod2 −/− mice express higher levels of chemokine and cytokine mRNA in the colon following DSS treatment

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Peptidoglycan recognition protein 3 and Nod2 synergistically protect mice from dextran sodium sulfate-induced colitis

    doi: 10.4049/jimmunol.1301548

    Figure Lengend Snippet: Pglyrp3 −/− Nod2 −/− mice express higher levels of chemokine and cytokine mRNA in the colon following DSS treatment

    Article Snippet: Experimental colitis was induced in WT, Nod2 −/− , Pglyrp3 −/− , and Pglyrp3 −/− Nod2 −/− mice with 5% DSS (dextran sulfate sodium, MP Biomedical) in drinking water ( ).

    Techniques: Mouse Assay

    Pglyrp3 −/− Nod2 −/− and Pglyrp3 −/− mice have higher levels of ATP in the colon, and intestinal ATP preferentially increases sensitivity of Nod2 −/− mice to DSS-colitis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Peptidoglycan recognition protein 3 and Nod2 synergistically protect mice from dextran sodium sulfate-induced colitis

    doi: 10.4049/jimmunol.1301548

    Figure Lengend Snippet: Pglyrp3 −/− Nod2 −/− and Pglyrp3 −/− mice have higher levels of ATP in the colon, and intestinal ATP preferentially increases sensitivity of Nod2 −/− mice to DSS-colitis

    Article Snippet: Experimental colitis was induced in WT, Nod2 −/− , Pglyrp3 −/− , and Pglyrp3 −/− Nod2 −/− mice with 5% DSS (dextran sulfate sodium, MP Biomedical) in drinking water ( ).

    Techniques: Mouse Assay

    Pglyrp3 −/− Nod2 −/− mice are more susceptible to DSS-induced colitis than Pglyrp3 and Nod2 single-deficient mice

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Peptidoglycan recognition protein 3 and Nod2 synergistically protect mice from dextran sodium sulfate-induced colitis

    doi: 10.4049/jimmunol.1301548

    Figure Lengend Snippet: Pglyrp3 −/− Nod2 −/− mice are more susceptible to DSS-induced colitis than Pglyrp3 and Nod2 single-deficient mice

    Article Snippet: Experimental colitis was induced in WT, Nod2 −/− , Pglyrp3 −/− , and Pglyrp3 −/− Nod2 −/− mice with 5% DSS (dextran sulfate sodium, MP Biomedical) in drinking water ( ).

    Techniques: Mouse Assay

    Klf5 +/− mice show impaired recovery during the DSS recovery phase. WT and Klf5 +/− mice were treated with DSS (3.5% and 2.5%, respectively) for 7 days to achieve similar degrees of colitis prior to 5 day recovery phase. (A) Weights of WT and Klf5 +/− mice after 7 days of DSS treatment and after 5 days of recovery (n = 14, * P = 0.02.) (B) Kaplan-Meier survival curve of WT and Klf5 +/− mice during DSS treatment and recovery phase (n = 14; P = 0.02). (C D) Clinical and histological scores after 7 days of DSS treatment or after 5 days of recovery. (C) n = 7; * P = 0.003; ** P = 0.05 (D) n = 7; * P

    Journal: Gastroenterology

    Article Title: Kr?ppel-Like Factor 5 Protects Against Dextran Sulfate Sodium-Induced Colonic Injury by Promoting Epithelial Repair in Mice

    doi: 10.1053/j.gastro.2010.10.061

    Figure Lengend Snippet: Klf5 +/− mice show impaired recovery during the DSS recovery phase. WT and Klf5 +/− mice were treated with DSS (3.5% and 2.5%, respectively) for 7 days to achieve similar degrees of colitis prior to 5 day recovery phase. (A) Weights of WT and Klf5 +/− mice after 7 days of DSS treatment and after 5 days of recovery (n = 14, * P = 0.02.) (B) Kaplan-Meier survival curve of WT and Klf5 +/− mice during DSS treatment and recovery phase (n = 14; P = 0.02). (C D) Clinical and histological scores after 7 days of DSS treatment or after 5 days of recovery. (C) n = 7; * P = 0.003; ** P = 0.05 (D) n = 7; * P

    Article Snippet: Colitis was induced in 7- to 8-week old gender-matched WT and Klf5+/− mice by by addition of dextran sulfate sodium (DSS) (molecular weight, 35,000–50,000; MP Biomedicals, Solon, OH) to the drinking water at 3.5% (wt/vol) for 7 days.

    Techniques: Mouse Assay

    EGFR levels are reduced at sites of ulceration in Klf5 +/− mice treated with DSS. (A) EGFR staining in colons of mice after 5 days of DSS treatment (acute phase); panel 1, WT; panel 2, Klf5 +/− . EGFR staining after 5 days of recovery from DSS treatment. Panel 3, WT (enlargement, panel 5); panel 4, Klf5 +/− (enlargement, panel 6) Red arrows indicate luminal epithelial cells. (B) Western blots of colon lysates from WT and Klf5 +/− mice treated with 3.5% DSS, days 0–5. Lysates are pooled from 3 separate mice. (C) Effects of Klf5 expression on EGFR levels in colon cancer cells. Western blot analysis with inhibition of KLF5 by siRNA in DLD-1 cells or overexpression of KLF5 in HCT 116 cells.

    Journal: Gastroenterology

    Article Title: Kr?ppel-Like Factor 5 Protects Against Dextran Sulfate Sodium-Induced Colonic Injury by Promoting Epithelial Repair in Mice

    doi: 10.1053/j.gastro.2010.10.061

    Figure Lengend Snippet: EGFR levels are reduced at sites of ulceration in Klf5 +/− mice treated with DSS. (A) EGFR staining in colons of mice after 5 days of DSS treatment (acute phase); panel 1, WT; panel 2, Klf5 +/− . EGFR staining after 5 days of recovery from DSS treatment. Panel 3, WT (enlargement, panel 5); panel 4, Klf5 +/− (enlargement, panel 6) Red arrows indicate luminal epithelial cells. (B) Western blots of colon lysates from WT and Klf5 +/− mice treated with 3.5% DSS, days 0–5. Lysates are pooled from 3 separate mice. (C) Effects of Klf5 expression on EGFR levels in colon cancer cells. Western blot analysis with inhibition of KLF5 by siRNA in DLD-1 cells or overexpression of KLF5 in HCT 116 cells.

    Article Snippet: Colitis was induced in 7- to 8-week old gender-matched WT and Klf5+/− mice by by addition of dextran sulfate sodium (DSS) (molecular weight, 35,000–50,000; MP Biomedicals, Solon, OH) to the drinking water at 3.5% (wt/vol) for 7 days.

    Techniques: Mouse Assay, Staining, Western Blot, Expressing, Inhibition, Over Expression

    Induction of KLF5 with DSS treatment is dependent on ERK1/2 signaling. (A) Detection of ERK1/2 and NF-κB activation by Western blotting, using pooled lysates from WT mice treated with 3.5% DSS over a 5 day time course (n=3). (B) Caco-2 cells were treated with 3% DSS for up to 8 hrs, and lysates were examined by Western blotting. (C) Caco-2 cells were pretreated with inhibitors of MEK/ERK (U0126) or NF-κB (Bay 11-7082), and treated for 5 hrs with 3% DSS. Lysates were examined by Western blotting.

    Journal: Gastroenterology

    Article Title: Kr?ppel-Like Factor 5 Protects Against Dextran Sulfate Sodium-Induced Colonic Injury by Promoting Epithelial Repair in Mice

    doi: 10.1053/j.gastro.2010.10.061

    Figure Lengend Snippet: Induction of KLF5 with DSS treatment is dependent on ERK1/2 signaling. (A) Detection of ERK1/2 and NF-κB activation by Western blotting, using pooled lysates from WT mice treated with 3.5% DSS over a 5 day time course (n=3). (B) Caco-2 cells were treated with 3% DSS for up to 8 hrs, and lysates were examined by Western blotting. (C) Caco-2 cells were pretreated with inhibitors of MEK/ERK (U0126) or NF-κB (Bay 11-7082), and treated for 5 hrs with 3% DSS. Lysates were examined by Western blotting.

    Article Snippet: Colitis was induced in 7- to 8-week old gender-matched WT and Klf5+/− mice by by addition of dextran sulfate sodium (DSS) (molecular weight, 35,000–50,000; MP Biomedicals, Solon, OH) to the drinking water at 3.5% (wt/vol) for 7 days.

    Techniques: Activation Assay, Western Blot, Mouse Assay

    Klf5 expression is reduced at sites of ulceration in DSS-treated Klf5 +/− mice. (A) Immunofluorescence staining of Klf5 in WT and Klf5 +/− mice untreated or treated with DSS for 7 days. White arrows indicate Klf5 staining at luminal surface (B) Quantification of fluorescent intensities of Klf5 staining per cell. Data represent the mean ± SEM of 5 mice per group with at least 50 cells quantified per sample. * P = 0.01; ** P = 0.04. (C) Quantification of Klf5-positive cells per crypt in untreated and DSS-treated WT and Klf5 +/− mice. Data represent the mean ± SEM of 3 mice per group, counting 5 crypts per mouse. * P

    Journal: Gastroenterology

    Article Title: Kr?ppel-Like Factor 5 Protects Against Dextran Sulfate Sodium-Induced Colonic Injury by Promoting Epithelial Repair in Mice

    doi: 10.1053/j.gastro.2010.10.061

    Figure Lengend Snippet: Klf5 expression is reduced at sites of ulceration in DSS-treated Klf5 +/− mice. (A) Immunofluorescence staining of Klf5 in WT and Klf5 +/− mice untreated or treated with DSS for 7 days. White arrows indicate Klf5 staining at luminal surface (B) Quantification of fluorescent intensities of Klf5 staining per cell. Data represent the mean ± SEM of 5 mice per group with at least 50 cells quantified per sample. * P = 0.01; ** P = 0.04. (C) Quantification of Klf5-positive cells per crypt in untreated and DSS-treated WT and Klf5 +/− mice. Data represent the mean ± SEM of 3 mice per group, counting 5 crypts per mouse. * P

    Article Snippet: Colitis was induced in 7- to 8-week old gender-matched WT and Klf5+/− mice by by addition of dextran sulfate sodium (DSS) (molecular weight, 35,000–50,000; MP Biomedicals, Solon, OH) to the drinking water at 3.5% (wt/vol) for 7 days.

    Techniques: Expressing, Mouse Assay, Immunofluorescence, Staining

    Klf5 +/− mice exhibit increased susceptibility to colitis with DSS treatment. Eight-week old WT and Klf5 +/− mice were treated with 3.5% DSS for 7 days and examined for clinical signs of colitis. Colon tissues were processed for enzymatic and histological analysis. (A) Weights of mice during 7 day treatment period; n = 9 mice per group, a : P

    Journal: Gastroenterology

    Article Title: Kr?ppel-Like Factor 5 Protects Against Dextran Sulfate Sodium-Induced Colonic Injury by Promoting Epithelial Repair in Mice

    doi: 10.1053/j.gastro.2010.10.061

    Figure Lengend Snippet: Klf5 +/− mice exhibit increased susceptibility to colitis with DSS treatment. Eight-week old WT and Klf5 +/− mice were treated with 3.5% DSS for 7 days and examined for clinical signs of colitis. Colon tissues were processed for enzymatic and histological analysis. (A) Weights of mice during 7 day treatment period; n = 9 mice per group, a : P

    Article Snippet: Colitis was induced in 7- to 8-week old gender-matched WT and Klf5+/− mice by by addition of dextran sulfate sodium (DSS) (molecular weight, 35,000–50,000; MP Biomedicals, Solon, OH) to the drinking water at 3.5% (wt/vol) for 7 days.

    Techniques: Mouse Assay

    Klf5 is induced in wild-type (WT) mice treated with DSS. Eight week-old WT mice were given water or treated with 3.5% DSS (wt/vol). (A) Immunohistochemical staining of colon of Klf5 in WT mice untreated or treated with DSS for 7 days (brown, Klf5; blue; counterstain). Red bars and arrows indicate regions of Klf5 expression. (B) Western blots of lysates from WT mice treated with DSS over a 5 day time course. Lysates for each time point were pooled from 3 separate mice.

    Journal: Gastroenterology

    Article Title: Kr?ppel-Like Factor 5 Protects Against Dextran Sulfate Sodium-Induced Colonic Injury by Promoting Epithelial Repair in Mice

    doi: 10.1053/j.gastro.2010.10.061

    Figure Lengend Snippet: Klf5 is induced in wild-type (WT) mice treated with DSS. Eight week-old WT mice were given water or treated with 3.5% DSS (wt/vol). (A) Immunohistochemical staining of colon of Klf5 in WT mice untreated or treated with DSS for 7 days (brown, Klf5; blue; counterstain). Red bars and arrows indicate regions of Klf5 expression. (B) Western blots of lysates from WT mice treated with DSS over a 5 day time course. Lysates for each time point were pooled from 3 separate mice.

    Article Snippet: Colitis was induced in 7- to 8-week old gender-matched WT and Klf5+/− mice by by addition of dextran sulfate sodium (DSS) (molecular weight, 35,000–50,000; MP Biomedicals, Solon, OH) to the drinking water at 3.5% (wt/vol) for 7 days.

    Techniques: Mouse Assay, Immunohistochemistry, Staining, Expressing, Western Blot

    Antibody responses to α-Glucans are impaired in the young and require IL-7 (A) Three-week-old (young) or 8- to 16-week-old (adult) C57BL/6 mice or adult IL-7 −/− mice were immunized with B512 dextran, and α1,6-dextran-specific IgM levels were measured by ELISA. (B) Peritoneal cavity cells of B512 dextran immunized mice (n = 5) were stained with antibodies specific for surface CD19, Mac1, CD5, and α1,6-dextran-FITC, and stained cells were analyzed by flow cytometry. All cells were first identified as CD19 + lymphocytes and the frequency of B1a (CD5 + , Mac1 + ), B1b (CD5 − , Mac1 + ) and B2 (CD5 − , Mac1 − ) subsets among the B cells is shown. (C) Young or adult BALB/cJ mice were immunized with Enterobacter cloacae strain MK7 (expressing α1,3-dextran epitope), and α1,3-dextran-specific IgM levels were measured by ELISA. Statistical significance for antibody responses to α1,6-dextran or to α1,3-dextran was measured using two-way ANOVA and Bonferroni post-test, and p values are shown in the plots. (D) Peritoneal cavity cells of Enterobacter cloacae strain MK7 immunized adult BALB/cJ mice (n = 5) were stained with antibodies, as described above, with the exception that α1,3-dextran-FITC was used rather than α1,6-dextran-FITC. The data are pooled from two independent experiments, consisting of 2-3 mice per group.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Interleukin-7 enables antibody responses to bacterial polysaccharides by promoting B cell antigen receptor diversity

    doi: 10.4049/jimmunol.1800162

    Figure Lengend Snippet: Antibody responses to α-Glucans are impaired in the young and require IL-7 (A) Three-week-old (young) or 8- to 16-week-old (adult) C57BL/6 mice or adult IL-7 −/− mice were immunized with B512 dextran, and α1,6-dextran-specific IgM levels were measured by ELISA. (B) Peritoneal cavity cells of B512 dextran immunized mice (n = 5) were stained with antibodies specific for surface CD19, Mac1, CD5, and α1,6-dextran-FITC, and stained cells were analyzed by flow cytometry. All cells were first identified as CD19 + lymphocytes and the frequency of B1a (CD5 + , Mac1 + ), B1b (CD5 − , Mac1 + ) and B2 (CD5 − , Mac1 − ) subsets among the B cells is shown. (C) Young or adult BALB/cJ mice were immunized with Enterobacter cloacae strain MK7 (expressing α1,3-dextran epitope), and α1,3-dextran-specific IgM levels were measured by ELISA. Statistical significance for antibody responses to α1,6-dextran or to α1,3-dextran was measured using two-way ANOVA and Bonferroni post-test, and p values are shown in the plots. (D) Peritoneal cavity cells of Enterobacter cloacae strain MK7 immunized adult BALB/cJ mice (n = 5) were stained with antibodies, as described above, with the exception that α1,3-dextran-FITC was used rather than α1,6-dextran-FITC. The data are pooled from two independent experiments, consisting of 2-3 mice per group.

    Article Snippet: For dextran immunizations, 50 μg of α1-6 dextran (B512; MP Biomedicals, LLC, Solon, OH) or 50 μg of α1-3 dextran (B1355; A gift from Dr. A. Jeanes) dissolved in 100 μl DPBS were used to immunize mice i.p. For whole bacterial immunization, mice were injected i.p. with 3×108 heat-killed Escherichia coli strain W3110 pDC5, which expresses ViPS , or 108 heat-killed, paraformaldehyde-fixed Enterobacter cloacae strain MK7, which expresses α1-3 dextran.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay, Staining, Flow Cytometry, Cytometry, Expressing

    Microbial-based therapies, QBKPN and QBECO, have different organ-specific anti-cancer efficacies. ( A ) Lung tumour count in a lung cancer model; lungs of C57BL/6 mice were seeded with Lewis Lung Carcinoma (LLC)-red fluorescent protein (RFP) cells by tail vein injection and treated with vehicle, QBKPN, or QBECO. N = 5 mice per group. Left lung tumour nodule counts were completed 15 days after tumor cell inoculation. ( B ) Survival in a gastrointestinal cancer model; intraperitoneal space of C57BL/6 mice were seeded with MC-38 adenocarcinoma cells by intraperitoneal injection and treated with vehicle, QBKPN, or QBECO. Survival curves to day 26 after tumour seeding. N = 10 mice per group. ( C ) Tumour counts in a spontaneous colon cancer model using C57BL/6 mice; cancer in the colon was induced with AOM/DSS exposure, and mice were treated with vehicle, QBECO or QBKPN. Tumour counts were performed at day 70 post-AOM treatment. N = 19–20 mice per group. For each experiment, mice were given the designated treatment by subcutaneous injection every second day starting 10 days before tumour seeding and continuing throughout the experiment. Data is shown with the mean ± SD. *p

    Journal: Scientific Reports

    Article Title: Distinct inactivated bacterial-based immune modulators vary in their therapeutic efficacies for treating disease based on the organ site of pathology

    doi: 10.1038/s41598-020-62735-z

    Figure Lengend Snippet: Microbial-based therapies, QBKPN and QBECO, have different organ-specific anti-cancer efficacies. ( A ) Lung tumour count in a lung cancer model; lungs of C57BL/6 mice were seeded with Lewis Lung Carcinoma (LLC)-red fluorescent protein (RFP) cells by tail vein injection and treated with vehicle, QBKPN, or QBECO. N = 5 mice per group. Left lung tumour nodule counts were completed 15 days after tumor cell inoculation. ( B ) Survival in a gastrointestinal cancer model; intraperitoneal space of C57BL/6 mice were seeded with MC-38 adenocarcinoma cells by intraperitoneal injection and treated with vehicle, QBKPN, or QBECO. Survival curves to day 26 after tumour seeding. N = 10 mice per group. ( C ) Tumour counts in a spontaneous colon cancer model using C57BL/6 mice; cancer in the colon was induced with AOM/DSS exposure, and mice were treated with vehicle, QBECO or QBKPN. Tumour counts were performed at day 70 post-AOM treatment. N = 19–20 mice per group. For each experiment, mice were given the designated treatment by subcutaneous injection every second day starting 10 days before tumour seeding and continuing throughout the experiment. Data is shown with the mean ± SD. *p

    Article Snippet: After AOM injections, mice received 3 cycles of dextran sodium sulphate (DSS) (MP Biomedicals, Santa Ana, CA, USA) treatment spaced 3 weeks apart.

    Techniques: Mouse Assay, Injection