puc19 (Valiant)
85
|
Name:
pUC19 plasmid
Description:
Puc19
Catalog Number:
04821227
Price:
283.4
Category:
Life Sciences Biochemicals
Size:
25 µg
|
Buy from Supplier |
Structured Review
Valiant
puc19
Puc19
https://www.bioz.com/result/puc19/product/Valiant
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Puc19
https://www.bioz.com/result/puc19/product/Valiant
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
puc19 - by Bioz Stars,
2021-03
85/100 stars
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Related Articles
Polymerase Chain Reaction:Article Title: Complete association between a retroviral insertion in the tyrosinase gene and the recessive white mutation in chickens Article Snippet: .. The long-range PCR amplification was performed in volumes of 50 μl containing 1 μl of each primer using 30 cycles under the following conditions: initial denaturation at 94°C for 2 min, 94°C for 10 sec (10 cycles) or 15 sec (20 cycles), 58°C for 30 sec, 68°C for 7 min, with a 20 sec additional extension for the last 20 cycles, followed by a final stage at 68°C for 7 min. After this amplification, six Hin dIII fragments of intron 4 of the recessive white chicken were cloned into the Article Title: Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities Article Snippet: B. subtilis ATCC 6633 was transformed by electroporation using the method of Dennis and Sokol ( ). .. For construction of the Amplification:Article Title: Complete association between a retroviral insertion in the tyrosinase gene and the recessive white mutation in chickens Article Snippet: .. The long-range PCR amplification was performed in volumes of 50 μl containing 1 μl of each primer using 30 cycles under the following conditions: initial denaturation at 94°C for 2 min, 94°C for 10 sec (10 cycles) or 15 sec (20 cycles), 58°C for 30 sec, 68°C for 7 min, with a 20 sec additional extension for the last 20 cycles, followed by a final stage at 68°C for 7 min. After this amplification, six Hin dIII fragments of intron 4 of the recessive white chicken were cloned into the Size-exclusion Chromatography:Article Title: Complete association between a retroviral insertion in the tyrosinase gene and the recessive white mutation in chickens Article Snippet: .. The long-range PCR amplification was performed in volumes of 50 μl containing 1 μl of each primer using 30 cycles under the following conditions: initial denaturation at 94°C for 2 min, 94°C for 10 sec (10 cycles) or 15 sec (20 cycles), 58°C for 30 sec, 68°C for 7 min, with a 20 sec additional extension for the last 20 cycles, followed by a final stage at 68°C for 7 min. After this amplification, six Hin dIII fragments of intron 4 of the recessive white chicken were cloned into the Clone Assay:Article Title: Complete association between a retroviral insertion in the tyrosinase gene and the recessive white mutation in chickens Article Snippet: .. The long-range PCR amplification was performed in volumes of 50 μl containing 1 μl of each primer using 30 cycles under the following conditions: initial denaturation at 94°C for 2 min, 94°C for 10 sec (10 cycles) or 15 sec (20 cycles), 58°C for 30 sec, 68°C for 7 min, with a 20 sec additional extension for the last 20 cycles, followed by a final stage at 68°C for 7 min. After this amplification, six Hin dIII fragments of intron 4 of the recessive white chicken were cloned into the Article Title: Characterization of new IS elements and studies of their dispersion in two subspecies of Leifsonia xyli Article Snippet: Genomic library construction, DNA sequencing and assembly Two DSMZ46306 genomic libraries were prepared. .. Genomic DNA extracted as previously described [ ] was mechanically sheared and the resulting fragments cloned into Article Title: Neuronal Genes for Subcutaneous Fat Thickness in Human and Pig Are Identified by Local Genomic Sequencing and Combined SNP Association Study Article Snippet: Small sizes of the fragments were removed using the Sizesep 400 spin column (Amersham Biosciences, USA) and CHROMA SPIN+TE1000 (Clontech, USA) and were subsequently repaired with DNA polymerase and the polynucleotide kinase method (BKL Kit; TaKaRa, Japan). .. The prepared DNA fragments were cloned into the dephosphorylated SmaI site of Plasmid Preparation:Article Title: Complete association between a retroviral insertion in the tyrosinase gene and the recessive white mutation in chickens Article Snippet: .. The long-range PCR amplification was performed in volumes of 50 μl containing 1 μl of each primer using 30 cycles under the following conditions: initial denaturation at 94°C for 2 min, 94°C for 10 sec (10 cycles) or 15 sec (20 cycles), 58°C for 30 sec, 68°C for 7 min, with a 20 sec additional extension for the last 20 cycles, followed by a final stage at 68°C for 7 min. After this amplification, six Hin dIII fragments of intron 4 of the recessive white chicken were cloned into the Article Title: A method for genotype validation and primer assessment in heterozygote-deficient species, as demonstrated in the prosobranch mollusc Hydrobia ulvae Article Snippet: The dinucleotide sequences (AC.GT)n and (AG.CT)n were obtained as DNA Alternating Copolymers (Amersham Pharmaceuticals Ltd, Buckinghamshire, UK) and the tetranucleotide sequences (TTTC.GAAA)n , (GTAA.TTAC)n , (GATA.TATC)n , (CTAA.TTAG)n and (TAAA.TTTA)n were prepared using two rounds of PCR amplification as in Armour et al. [ ]. .. Once recovered, the microsatellite-enriched Hydrobia ulvae fragments were separated from the Sau-L linkers by digestion with Mbo I. Fragments were then ligated into Bam HI/BAP-dephosphorylated Article Title: Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities Article Snippet: B. subtilis ATCC 6633 was transformed by electroporation using the method of Dennis and Sokol ( ). .. For construction of the Transformation Assay:Article Title: A method for genotype validation and primer assessment in heterozygote-deficient species, as demonstrated in the prosobranch mollusc Hydrobia ulvae Article Snippet: The dinucleotide sequences (AC.GT)n and (AG.CT)n were obtained as DNA Alternating Copolymers (Amersham Pharmaceuticals Ltd, Buckinghamshire, UK) and the tetranucleotide sequences (TTTC.GAAA)n , (GTAA.TTAC)n , (GATA.TATC)n , (CTAA.TTAG)n and (TAAA.TTTA)n were prepared using two rounds of PCR amplification as in Armour et al. [ ]. .. Once recovered, the microsatellite-enriched Hydrobia ulvae fragments were separated from the Sau-L linkers by digestion with Mbo I. Fragments were then ligated into Bam HI/BAP-dephosphorylated Homologous Recombination:Article Title: Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities Article Snippet: B. subtilis ATCC 6633 was transformed by electroporation using the method of Dennis and Sokol ( ). .. For construction of the Generated:Article Title: Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities Article Snippet: B. subtilis ATCC 6633 was transformed by electroporation using the method of Dennis and Sokol ( ). .. For construction of the |