puc19  (Valiant)

 
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  • 85
    Name:
    pUC19 plasmid
    Description:
    Puc19
    Catalog Number:
    04821227
    Price:
    283.4
    Category:
    Life Sciences Biochemicals
    Size:
    25 µg
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    Structured Review

    Valiant puc19
    Puc19
    https://www.bioz.com/result/puc19/product/Valiant
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    puc19 - by Bioz Stars, 2021-03
    85/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Complete association between a retroviral insertion in the tyrosinase gene and the recessive white mutation in chickens
    Article Snippet: .. The long-range PCR amplification was performed in volumes of 50 μl containing 1 μl of each primer using 30 cycles under the following conditions: initial denaturation at 94°C for 2 min, 94°C for 10 sec (10 cycles) or 15 sec (20 cycles), 58°C for 30 sec, 68°C for 7 min, with a 20 sec additional extension for the last 20 cycles, followed by a final stage at 68°C for 7 min. After this amplification, six Hin dIII fragments of intron 4 of the recessive white chicken were cloned into the pUC19 plasmid (Qbiogene, France). .. Two of the intronic Hin dIII fragments (3.2 kb and 3 kb) of the recessive white chicken were sent to be sequenced by the MWG Biotech Company (Germany) under the Publication Quality Sequencing project.

    Article Title: Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities
    Article Snippet: B. subtilis ATCC 6633 was transformed by electroporation using the method of Dennis and Sokol ( ). .. For construction of the pUC19-derived plasmid dedicated to promoter exchange by homologous recombination in B. subtilis , the pbp and fenF fragments were generated by PCR using Taq polymerase “Arrow” from Qbiogene (Montreal, Canada). .. The primers were designed by using the previously published sequence of the mycosubtilin operon from strain ATCC 6633 (PubMed nucleotide accession no. ) ( ).

    Amplification:

    Article Title: Complete association between a retroviral insertion in the tyrosinase gene and the recessive white mutation in chickens
    Article Snippet: .. The long-range PCR amplification was performed in volumes of 50 μl containing 1 μl of each primer using 30 cycles under the following conditions: initial denaturation at 94°C for 2 min, 94°C for 10 sec (10 cycles) or 15 sec (20 cycles), 58°C for 30 sec, 68°C for 7 min, with a 20 sec additional extension for the last 20 cycles, followed by a final stage at 68°C for 7 min. After this amplification, six Hin dIII fragments of intron 4 of the recessive white chicken were cloned into the pUC19 plasmid (Qbiogene, France). .. Two of the intronic Hin dIII fragments (3.2 kb and 3 kb) of the recessive white chicken were sent to be sequenced by the MWG Biotech Company (Germany) under the Publication Quality Sequencing project.

    Size-exclusion Chromatography:

    Article Title: Complete association between a retroviral insertion in the tyrosinase gene and the recessive white mutation in chickens
    Article Snippet: .. The long-range PCR amplification was performed in volumes of 50 μl containing 1 μl of each primer using 30 cycles under the following conditions: initial denaturation at 94°C for 2 min, 94°C for 10 sec (10 cycles) or 15 sec (20 cycles), 58°C for 30 sec, 68°C for 7 min, with a 20 sec additional extension for the last 20 cycles, followed by a final stage at 68°C for 7 min. After this amplification, six Hin dIII fragments of intron 4 of the recessive white chicken were cloned into the pUC19 plasmid (Qbiogene, France). .. Two of the intronic Hin dIII fragments (3.2 kb and 3 kb) of the recessive white chicken were sent to be sequenced by the MWG Biotech Company (Germany) under the Publication Quality Sequencing project.

    Clone Assay:

    Article Title: Complete association between a retroviral insertion in the tyrosinase gene and the recessive white mutation in chickens
    Article Snippet: .. The long-range PCR amplification was performed in volumes of 50 μl containing 1 μl of each primer using 30 cycles under the following conditions: initial denaturation at 94°C for 2 min, 94°C for 10 sec (10 cycles) or 15 sec (20 cycles), 58°C for 30 sec, 68°C for 7 min, with a 20 sec additional extension for the last 20 cycles, followed by a final stage at 68°C for 7 min. After this amplification, six Hin dIII fragments of intron 4 of the recessive white chicken were cloned into the pUC19 plasmid (Qbiogene, France). .. Two of the intronic Hin dIII fragments (3.2 kb and 3 kb) of the recessive white chicken were sent to be sequenced by the MWG Biotech Company (Germany) under the Publication Quality Sequencing project.

    Article Title: Characterization of new IS elements and studies of their dispersion in two subspecies of Leifsonia xyli
    Article Snippet: Genomic library construction, DNA sequencing and assembly Two DSMZ46306 genomic libraries were prepared. .. Genomic DNA extracted as previously described [ ] was mechanically sheared and the resulting fragments cloned into pUC19 (Q-Biogene, Carlsbad, CA). .. A BAC clone library was prepared with 25 kbp-inserts obtained by partial digestion with Bam HI [ ] cloned into pIndigoBAC-5 (Epicentre® ).

    Article Title: Neuronal Genes for Subcutaneous Fat Thickness in Human and Pig Are Identified by Local Genomic Sequencing and Combined SNP Association Study
    Article Snippet: Small sizes of the fragments were removed using the Sizesep 400 spin column (Amersham Biosciences, USA) and CHROMA SPIN+TE1000 (Clontech, USA) and were subsequently repaired with DNA polymerase and the polynucleotide kinase method (BKL Kit; TaKaRa, Japan). .. The prepared DNA fragments were cloned into the dephosphorylated SmaI site of pUC19 (Qbiogene, USA). .. Ligates were transformed into DH10B by electroporation (Gene Pulser II, Bio-Rad, USA).

    Plasmid Preparation:

    Article Title: Complete association between a retroviral insertion in the tyrosinase gene and the recessive white mutation in chickens
    Article Snippet: .. The long-range PCR amplification was performed in volumes of 50 μl containing 1 μl of each primer using 30 cycles under the following conditions: initial denaturation at 94°C for 2 min, 94°C for 10 sec (10 cycles) or 15 sec (20 cycles), 58°C for 30 sec, 68°C for 7 min, with a 20 sec additional extension for the last 20 cycles, followed by a final stage at 68°C for 7 min. After this amplification, six Hin dIII fragments of intron 4 of the recessive white chicken were cloned into the pUC19 plasmid (Qbiogene, France). .. Two of the intronic Hin dIII fragments (3.2 kb and 3 kb) of the recessive white chicken were sent to be sequenced by the MWG Biotech Company (Germany) under the Publication Quality Sequencing project.

    Article Title: A method for genotype validation and primer assessment in heterozygote-deficient species, as demonstrated in the prosobranch mollusc Hydrobia ulvae
    Article Snippet: The dinucleotide sequences (AC.GT)n and (AG.CT)n were obtained as DNA Alternating Copolymers (Amersham Pharmaceuticals Ltd, Buckinghamshire, UK) and the tetranucleotide sequences (TTTC.GAAA)n , (GTAA.TTAC)n , (GATA.TATC)n , (CTAA.TTAG)n and (TAAA.TTTA)n were prepared using two rounds of PCR amplification as in Armour et al. [ ]. .. Once recovered, the microsatellite-enriched Hydrobia ulvae fragments were separated from the Sau-L linkers by digestion with Mbo I. Fragments were then ligated into Bam HI/BAP-dephosphorylated pUC19 vector (Qbiogene) and transformed into XL1-Blue competent cells (Stratagene). ..

    Article Title: Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities
    Article Snippet: B. subtilis ATCC 6633 was transformed by electroporation using the method of Dennis and Sokol ( ). .. For construction of the pUC19-derived plasmid dedicated to promoter exchange by homologous recombination in B. subtilis , the pbp and fenF fragments were generated by PCR using Taq polymerase “Arrow” from Qbiogene (Montreal, Canada). .. The primers were designed by using the previously published sequence of the mycosubtilin operon from strain ATCC 6633 (PubMed nucleotide accession no. ) ( ).

    Transformation Assay:

    Article Title: A method for genotype validation and primer assessment in heterozygote-deficient species, as demonstrated in the prosobranch mollusc Hydrobia ulvae
    Article Snippet: The dinucleotide sequences (AC.GT)n and (AG.CT)n were obtained as DNA Alternating Copolymers (Amersham Pharmaceuticals Ltd, Buckinghamshire, UK) and the tetranucleotide sequences (TTTC.GAAA)n , (GTAA.TTAC)n , (GATA.TATC)n , (CTAA.TTAG)n and (TAAA.TTTA)n were prepared using two rounds of PCR amplification as in Armour et al. [ ]. .. Once recovered, the microsatellite-enriched Hydrobia ulvae fragments were separated from the Sau-L linkers by digestion with Mbo I. Fragments were then ligated into Bam HI/BAP-dephosphorylated pUC19 vector (Qbiogene) and transformed into XL1-Blue competent cells (Stratagene). ..

    Homologous Recombination:

    Article Title: Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities
    Article Snippet: B. subtilis ATCC 6633 was transformed by electroporation using the method of Dennis and Sokol ( ). .. For construction of the pUC19-derived plasmid dedicated to promoter exchange by homologous recombination in B. subtilis , the pbp and fenF fragments were generated by PCR using Taq polymerase “Arrow” from Qbiogene (Montreal, Canada). .. The primers were designed by using the previously published sequence of the mycosubtilin operon from strain ATCC 6633 (PubMed nucleotide accession no. ) ( ).

    Generated:

    Article Title: Mycosubtilin Overproduction by Bacillus subtilis BBG100 Enhances the Organism's Antagonistic and Biocontrol Activities
    Article Snippet: B. subtilis ATCC 6633 was transformed by electroporation using the method of Dennis and Sokol ( ). .. For construction of the pUC19-derived plasmid dedicated to promoter exchange by homologous recombination in B. subtilis , the pbp and fenF fragments were generated by PCR using Taq polymerase “Arrow” from Qbiogene (Montreal, Canada). .. The primers were designed by using the previously published sequence of the mycosubtilin operon from strain ATCC 6633 (PubMed nucleotide accession no. ) ( ).

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  • 85
    Valiant puc19 derived plasmid
    Puc19 Derived Plasmid, supplied by Valiant, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/puc19 derived plasmid/product/Valiant
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    puc19 derived plasmid - by Bioz Stars, 2021-03
    85/100 stars
      Buy from Supplier

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