concanavalin a  (Valiant)


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    Name:
    Concanavalin A from Canavalia ensiformis seeds
    Description:
    Concanavalin A
    Catalog Number:
    02150710-cf
    Price:
    10.0
    Applications:
    Cell Biology, Cell Signaling
    Category:
    Life Sciences Biochemicals Proteins and Derivatives
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    Structured Review

    Valiant concanavalin a
    SPR sensorgrams (without background subtraction) of non-specific BSA (a) and specific Con A (b) binding to mannose functionalized chips with different underlying chemistries. C 11  substrate: 11-mercaptoundecanol, (OEG) 3 -C 11  substrate: (11-mercaptoundecyl)
    Concanavalin A
    https://www.bioz.com/result/concanavalin a/product/Valiant
    Average 93 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    concanavalin a - by Bioz Stars, 2021-02
    93/100 stars

    Images

    1) Product Images from "A Versatile Method for Functionalizing Surfaces with Bioactive Glycans"

    Article Title: A Versatile Method for Functionalizing Surfaces with Bioactive Glycans

    Journal: Bioconjugate chemistry

    doi: 10.1021/bc1003372

    SPR sensorgrams (without background subtraction) of non-specific BSA (a) and specific Con A (b) binding to mannose functionalized chips with different underlying chemistries. C 11  substrate: 11-mercaptoundecanol, (OEG) 3 -C 11  substrate: (11-mercaptoundecyl)
    Figure Legend Snippet: SPR sensorgrams (without background subtraction) of non-specific BSA (a) and specific Con A (b) binding to mannose functionalized chips with different underlying chemistries. C 11 substrate: 11-mercaptoundecanol, (OEG) 3 -C 11 substrate: (11-mercaptoundecyl)

    Techniques Used: SPR Assay, Binding Assay

    SPRi shows that the response of Con A varies as a function of the nucleophile used for conjugation of mannose to the surface.
    Figure Legend Snippet: SPRi shows that the response of Con A varies as a function of the nucleophile used for conjugation of mannose to the surface.

    Techniques Used: Conjugation Assay

    2) Product Images from "Essential gene disruptions reveal complex relationships between phenotypic robustness, pleiotropy, and fitness"

    Article Title: Essential gene disruptions reveal complex relationships between phenotypic robustness, pleiotropy, and fitness

    Journal: Molecular Systems Biology

    doi: 10.15252/msb.20145264

    Example images of mutants with high or low levels of phenotypic variation Cell walls are labeled with FITC-conjugated concanavalin A (magenta) and nuclei are labeled with DAPI (green). DAmP-YDR355C (phenotypic potential = 3.25), DAmP-RNA1 (phenotypic potential = 1.81), DAmP-NOP1 (phenotypic potential = 3.07), DAmP-IWS1 (phenotypic potential = 2.14), DAmP-CDC42 (phenotypic potential = 2.54), and DAmP-ERG12 (phenotypic potential = 1.61) are all high-confidence phenotypic stabilizers. R1158 (phenotypic potential = −0.05) does not contain any essential gene mutations. DAmP-MEX67 (phenotypic potential = −1.42) and DAmP-FBA1 (phenotypic potential = −1.01) are DAmP strains that display low phenotypic variation.
    Figure Legend Snippet: Example images of mutants with high or low levels of phenotypic variation Cell walls are labeled with FITC-conjugated concanavalin A (magenta) and nuclei are labeled with DAPI (green). DAmP-YDR355C (phenotypic potential = 3.25), DAmP-RNA1 (phenotypic potential = 1.81), DAmP-NOP1 (phenotypic potential = 3.07), DAmP-IWS1 (phenotypic potential = 2.14), DAmP-CDC42 (phenotypic potential = 2.54), and DAmP-ERG12 (phenotypic potential = 1.61) are all high-confidence phenotypic stabilizers. R1158 (phenotypic potential = −0.05) does not contain any essential gene mutations. DAmP-MEX67 (phenotypic potential = −1.42) and DAmP-FBA1 (phenotypic potential = −1.01) are DAmP strains that display low phenotypic variation.

    Techniques Used: Labeling

    3) Product Images from "Feeding a Diet Enriched in Docosahexaenoic Acid to Lactating Dams Improves the Tolerance Response to Egg Protein in Suckled Pups"

    Article Title: Feeding a Diet Enriched in Docosahexaenoic Acid to Lactating Dams Improves the Tolerance Response to Egg Protein in Suckled Pups

    Journal: Nutrients

    doi: 10.3390/nu8020103

    Summary of the effects of feeding a high DHA maternal diet during the suckling period and the mucosal oral tolerance treatment on the development of the immune system in offspring. (ConA, Concanavalin A; DHA, docosahexaenoic acid; IFN-γ, interferon-gamma; Ig, immunoglobulin; IL, interleukin; LPS, lipopolysaccharide; OT, oral tolerance; OVA, ovalbumin; TGF-β, transforming growth factor-beta; TNF-α, tumor necrosis factor-alpha).
    Figure Legend Snippet: Summary of the effects of feeding a high DHA maternal diet during the suckling period and the mucosal oral tolerance treatment on the development of the immune system in offspring. (ConA, Concanavalin A; DHA, docosahexaenoic acid; IFN-γ, interferon-gamma; Ig, immunoglobulin; IL, interleukin; LPS, lipopolysaccharide; OT, oral tolerance; OVA, ovalbumin; TGF-β, transforming growth factor-beta; TNF-α, tumor necrosis factor-alpha).

    Techniques Used:

    4) Product Images from "Impaired liver regeneration is associated with reduced cyclin B1 in natural killer T cell-deficient mice"

    Article Title: Impaired liver regeneration is associated with reduced cyclin B1 in natural killer T cell-deficient mice

    Journal: BMC Gastroenterology

    doi: 10.1186/s12876-017-0600-2

    The effect of ConA on liver injury after partial hepatectomy. a ConA was intravenously injected to CD1d-/- or to wild type mice 4 days prior to 70% PH. In other experiments vehicle was injected instead of ConA in the same setting. Serum ALT levels were measured 48 h after PH. b Anti CD1d or isotype control antibodies were intravenously injected 1 h before ConA administration 4 days prior to 70% PH. In other experiments vehicle was injected instead of ConA in the same setting. Serum ALT levels were measured 48 h after PH. c As in A, but ALT levels were measured 3 h after PH. Bars are means ± standard error values. N = 9 mice per condition. ***, p
    Figure Legend Snippet: The effect of ConA on liver injury after partial hepatectomy. a ConA was intravenously injected to CD1d-/- or to wild type mice 4 days prior to 70% PH. In other experiments vehicle was injected instead of ConA in the same setting. Serum ALT levels were measured 48 h after PH. b Anti CD1d or isotype control antibodies were intravenously injected 1 h before ConA administration 4 days prior to 70% PH. In other experiments vehicle was injected instead of ConA in the same setting. Serum ALT levels were measured 48 h after PH. c As in A, but ALT levels were measured 3 h after PH. Bars are means ± standard error values. N = 9 mice per condition. ***, p

    Techniques Used: Injection, Mouse Assay

    Expression patterns of hepatic PCNA, P21 and Cyclin B1 during liver regeneration from ConA-challenged isotype control and anti CD1d-treated mice. a Western blotting analysis of hepatic remnant lysates, as described in materials and methods, was performed 48 h after PH using anti PCNA, anti p21 and anti cyclinB1. β-actin was used as loading control. The illustrated bands are representative of 3 mice per group. b Densitometry of PCNA c Densitometry of Cyclin B1 d Densitometry of p21. Bars are means ± standard error values of intensity for individual bands that were quantified using EZQuant-Gel densitometry software, and expressed relative to β-actin, as a measure of protein relative abundance in the different liver samples. * p
    Figure Legend Snippet: Expression patterns of hepatic PCNA, P21 and Cyclin B1 during liver regeneration from ConA-challenged isotype control and anti CD1d-treated mice. a Western blotting analysis of hepatic remnant lysates, as described in materials and methods, was performed 48 h after PH using anti PCNA, anti p21 and anti cyclinB1. β-actin was used as loading control. The illustrated bands are representative of 3 mice per group. b Densitometry of PCNA c Densitometry of Cyclin B1 d Densitometry of p21. Bars are means ± standard error values of intensity for individual bands that were quantified using EZQuant-Gel densitometry software, and expressed relative to β-actin, as a measure of protein relative abundance in the different liver samples. * p

    Techniques Used: Expressing, Mouse Assay, Western Blot, Software

    The effect of ConA on liver regeneration in the absence of NKT cells. a ConA was intravenously injected to CD1d-/- or to wild type mice 4 days prior to 70% PH. In other the experiments vehicle was injected instead of ConA in the same setting. Hepatocellular proliferation was measured by Ki-67 48 h after PH. b Anti CD1d or isotype control antibodies were intravenously injected 1 h before ConA administration 4 days prior to 70% PH. In the other experiments vehicle was injected instead of ConA in the same setting. Data represent means ± standard error of a representative experiment. N = 3 mice or more per condition. All experiments were repeated 3-4 times. In CD1d-/- experiments, * p
    Figure Legend Snippet: The effect of ConA on liver regeneration in the absence of NKT cells. a ConA was intravenously injected to CD1d-/- or to wild type mice 4 days prior to 70% PH. In other the experiments vehicle was injected instead of ConA in the same setting. Hepatocellular proliferation was measured by Ki-67 48 h after PH. b Anti CD1d or isotype control antibodies were intravenously injected 1 h before ConA administration 4 days prior to 70% PH. In the other experiments vehicle was injected instead of ConA in the same setting. Data represent means ± standard error of a representative experiment. N = 3 mice or more per condition. All experiments were repeated 3-4 times. In CD1d-/- experiments, * p

    Techniques Used: Injection, Mouse Assay

    The effect of partial hepatectomy on serum IL-6 secretion after ConA challenge. a ConA was intravenously injected to CD1d-/- or to wild type mice 4 days prior to 70% PH. In the other experiments vehicle was injected instead of ConA in the same setting. Serum IL-6 levels were measured 3 h after PH by ELISA ( a ). b As in A, but IL-6 levels were measured 48 h after PH. c Anti CD1d or isotype control antibodies were intravenously injected 1 h before ConA administration 4 days prior to 70% PH. In the other experiments vehicle was injected instead of ConA in the same setting. Serum IL-6 levels were measured 48 h after PH. Bars are means ± standard error values. N = 9 mice per condition. * p
    Figure Legend Snippet: The effect of partial hepatectomy on serum IL-6 secretion after ConA challenge. a ConA was intravenously injected to CD1d-/- or to wild type mice 4 days prior to 70% PH. In the other experiments vehicle was injected instead of ConA in the same setting. Serum IL-6 levels were measured 3 h after PH by ELISA ( a ). b As in A, but IL-6 levels were measured 48 h after PH. c Anti CD1d or isotype control antibodies were intravenously injected 1 h before ConA administration 4 days prior to 70% PH. In the other experiments vehicle was injected instead of ConA in the same setting. Serum IL-6 levels were measured 48 h after PH. Bars are means ± standard error values. N = 9 mice per condition. * p

    Techniques Used: Injection, Mouse Assay, Enzyme-linked Immunosorbent Assay

    5) Product Images from "A Model for Cell Wall Dissolution in Mating Yeast Cells: Polarized Secretion and Restricted Diffusion of Cell Wall Remodeling Enzymes Induces Local Dissolution"

    Article Title: A Model for Cell Wall Dissolution in Mating Yeast Cells: Polarized Secretion and Restricted Diffusion of Cell Wall Remodeling Enzymes Induces Local Dissolution

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0109780

    Pheromone-induced cell death increases with increasing attachments to an impermeable surface. A. Cells grown in bulk culture were incubated in test tubes on roller drums in liquid media without any enforced contact with impermeable surfaces. Cells grown in a concanavalin A (ConA) chamber were grown in a chamber whose depth was many times the diameter of a single yeast cell and attached to a single surface of the chamber (the ceiling provided by a glass coverslip) using the lectin, concanavalin A. For confinement, cells were loaded into a microfluidic chamber which traps cells between a ceiling and floor separated by the diameter of a single yeast cell, causing enforced contact with two surfaces. Medium is then constantly perfused through the chamber. B. Percent of MAT a bar1Δ cells that died after exposure to 50nM α-factor for five hours in three different physical environments. Error bars represent the standard deviation of at least three independent experiments. C. Time course of MAT a bar1Δ cells incubated in 50nM α-factor for the indicated amount of time in the flow chamber. Yellow arrows indicate cells that died since the previous time point. White arrows indicate cells that died earlier. The scale bar indicates 10 µm.
    Figure Legend Snippet: Pheromone-induced cell death increases with increasing attachments to an impermeable surface. A. Cells grown in bulk culture were incubated in test tubes on roller drums in liquid media without any enforced contact with impermeable surfaces. Cells grown in a concanavalin A (ConA) chamber were grown in a chamber whose depth was many times the diameter of a single yeast cell and attached to a single surface of the chamber (the ceiling provided by a glass coverslip) using the lectin, concanavalin A. For confinement, cells were loaded into a microfluidic chamber which traps cells between a ceiling and floor separated by the diameter of a single yeast cell, causing enforced contact with two surfaces. Medium is then constantly perfused through the chamber. B. Percent of MAT a bar1Δ cells that died after exposure to 50nM α-factor for five hours in three different physical environments. Error bars represent the standard deviation of at least three independent experiments. C. Time course of MAT a bar1Δ cells incubated in 50nM α-factor for the indicated amount of time in the flow chamber. Yellow arrows indicate cells that died since the previous time point. White arrows indicate cells that died earlier. The scale bar indicates 10 µm.

    Techniques Used: Incubation, Standard Deviation, Flow Cytometry

    6) Product Images from "Statins accelerate the onset of collagen type II-induced arthritis in mice"

    Article Title: Statins accelerate the onset of collagen type II-induced arthritis in mice

    Journal: Arthritis Research & Therapy

    doi: 10.1186/ar3814

    Cytokine production by lymph node and spleen cells . (A) IL-2 production by Concanavalin-A (Con A)-stimulated inguinal lymph node (LN) cells. (B) IL-2 production by collagen type II (CII)-stimulated spleen cells. (C) IL-17 production by CII-stimulated spleen cells. (D) IFNγ production by CII-stimulated spleen cells. Mice were immunized at day 0 and challenged at day 21. Atorvastatin (A) or pravastatin (P) were given daily, either before (day -28 until day 20) or after (day 22 until day 42) collagen-induced arthritis induction. Mice were euthanized at day 42. Control mice (C) did not receive statins. In negative mice (N), arthritis was not induced and these mice did not receive statins. Inguinal LNs were excised and LN cells were cultured in the presence of Con A for 24 hours. Spleens were excised and spleen cells were cultured in the presence of CII for 72 hours. Cytokine content was measured in the supernatants. n = 12. *Significantly different ( P
    Figure Legend Snippet: Cytokine production by lymph node and spleen cells . (A) IL-2 production by Concanavalin-A (Con A)-stimulated inguinal lymph node (LN) cells. (B) IL-2 production by collagen type II (CII)-stimulated spleen cells. (C) IL-17 production by CII-stimulated spleen cells. (D) IFNγ production by CII-stimulated spleen cells. Mice were immunized at day 0 and challenged at day 21. Atorvastatin (A) or pravastatin (P) were given daily, either before (day -28 until day 20) or after (day 22 until day 42) collagen-induced arthritis induction. Mice were euthanized at day 42. Control mice (C) did not receive statins. In negative mice (N), arthritis was not induced and these mice did not receive statins. Inguinal LNs were excised and LN cells were cultured in the presence of Con A for 24 hours. Spleens were excised and spleen cells were cultured in the presence of CII for 72 hours. Cytokine content was measured in the supernatants. n = 12. *Significantly different ( P

    Techniques Used: Mouse Assay, Cell Culture

    7) Product Images from "Glycosylated Self-Assembled Monolayers for Arrays and Surface Analysis"

    Article Title: Glycosylated Self-Assembled Monolayers for Arrays and Surface Analysis

    Journal: Methods in molecular biology (Clifton, N.J.)

    doi: 10.1007/978-1-61779-373-8_6

    SPRi reflectivity image and the corresponding sensorgrams on a spotted glyco-SAM array composed of thiolated mannose, galactose and the glycoprotein RNase B. The bioactivity of immobilized glycans were verified via lectin binding with Con A (500 nM).
    Figure Legend Snippet: SPRi reflectivity image and the corresponding sensorgrams on a spotted glyco-SAM array composed of thiolated mannose, galactose and the glycoprotein RNase B. The bioactivity of immobilized glycans were verified via lectin binding with Con A (500 nM).

    Techniques Used: Binding Assay

    8) Product Images from "The crystal structure of titanium dioxide nanoparticles influences immune activity in vitro and in vivo"

    Article Title: The crystal structure of titanium dioxide nanoparticles influences immune activity in vitro and in vivo

    Journal: Particle and Fibre Toxicology

    doi: 10.1186/s12989-018-0245-5

    a . IL-4 production by LN cells.  b . IL-5 production by LN cells. Mice were sensitized with OVA alone, OVA + rutile NP, OVA + anatase NP, or OVA + CB, and challenged with OVA. LN cell preparations were made and incubated with Con A for 24 h. N = 6, mean ± SEM is shown. (*)  P
    Figure Legend Snippet: a . IL-4 production by LN cells. b . IL-5 production by LN cells. Mice were sensitized with OVA alone, OVA + rutile NP, OVA + anatase NP, or OVA + CB, and challenged with OVA. LN cell preparations were made and incubated with Con A for 24 h. N = 6, mean ± SEM is shown. (*) P

    Techniques Used: Mouse Assay, Incubation

    9) Product Images from "Concanavalin A Toxicity Towards Potato Psyllid and Apoptosis Induction in Midgut Cells"

    Article Title: Concanavalin A Toxicity Towards Potato Psyllid and Apoptosis Induction in Midgut Cells

    Journal: Insects

    doi: 10.3390/insects11040243

    Feeding assays and Concanavalin A (ConA) toxicity towards potato psyllid. ( a ) Apparatus for feeding assays. The chambers were covered by two sheets of Parafilm with liquid diet between the two layers. ( b ) Mortality of Candidatus Liberibacter solanacearum (Lso)-free, LsoA-, and LsoB-infected potato psyllids following feeding on artificial diets without (control) and with ConA at the concentration of 2000 μg/mL. p -value refers to the log-rank test. The gray region indicates a significant mortality of psyllids after feeding ConA.
    Figure Legend Snippet: Feeding assays and Concanavalin A (ConA) toxicity towards potato psyllid. ( a ) Apparatus for feeding assays. The chambers were covered by two sheets of Parafilm with liquid diet between the two layers. ( b ) Mortality of Candidatus Liberibacter solanacearum (Lso)-free, LsoA-, and LsoB-infected potato psyllids following feeding on artificial diets without (control) and with ConA at the concentration of 2000 μg/mL. p -value refers to the log-rank test. The gray region indicates a significant mortality of psyllids after feeding ConA.

    Techniques Used: Infection, Concentration Assay

    10) Product Images from "The role of Toll-like receptor-4 in pertussis vaccine-induced immunity"

    Article Title: The role of Toll-like receptor-4 in pertussis vaccine-induced immunity

    Journal: BMC Immunology

    doi: 10.1186/1471-2172-9-21

    Ex vivo  cytokine production by bronchial lymph node and spleen cells .  Tlr4 -deficient and control mice were sc injected with 1/5 HD wP (|▮|), aP (|▒|), or adjuvant (| |), twice before intranasal  B. pertussis  infection. Three and seven days after challenge the bronchial lymph nodes (LN) and spleens were excised and cell suspensions were made. Bronchial LN cells were cultured with Con A for 24 hr; spleen cells were cultured with heat-killed  B. pertussis  for 72 hr. Culture supernatants were analyzed for cytokine content by Luminex. Data are indicated as mean ± SEM (N = 6). Non-boxed  P -values: compared to the adjuvant control, or (when indicated) aP-vaccinated group (same strain and day after challenge). Boxed  P -values: compared to wild-type mice (same treatment and day after challenge). ANOVA followed by Bonferroni. A single representative experiment of 2 is shown.
    Figure Legend Snippet: Ex vivo cytokine production by bronchial lymph node and spleen cells . Tlr4 -deficient and control mice were sc injected with 1/5 HD wP (|▮|), aP (|▒|), or adjuvant (| |), twice before intranasal B. pertussis infection. Three and seven days after challenge the bronchial lymph nodes (LN) and spleens were excised and cell suspensions were made. Bronchial LN cells were cultured with Con A for 24 hr; spleen cells were cultured with heat-killed B. pertussis for 72 hr. Culture supernatants were analyzed for cytokine content by Luminex. Data are indicated as mean ± SEM (N = 6). Non-boxed P -values: compared to the adjuvant control, or (when indicated) aP-vaccinated group (same strain and day after challenge). Boxed P -values: compared to wild-type mice (same treatment and day after challenge). ANOVA followed by Bonferroni. A single representative experiment of 2 is shown.

    Techniques Used: Ex Vivo, Mouse Assay, Injection, Infection, Cell Culture, Luminex

    11) Product Images from "?-Glycoglycosphingolipid-Induced Alterations of the STAT Signaling Pathways Are Dependent on CD1d and the Lipid Raft Protein Flotillin-2"

    Article Title: ?-Glycoglycosphingolipid-Induced Alterations of the STAT Signaling Pathways Are Dependent on CD1d and the Lipid Raft Protein Flotillin-2

    Journal: The American Journal of Pathology

    doi: 10.2353/ajpath.2009.080841

    A:  Effects of different ratios and doses of β-ligands on phosphorylated STAT1, STAT4, and STAT6 levels in the mouse Con A hepatitis model. Treatments A–M are specified in .  B:  Effects of different ratios and doses of β-ligands
    Figure Legend Snippet: A: Effects of different ratios and doses of β-ligands on phosphorylated STAT1, STAT4, and STAT6 levels in the mouse Con A hepatitis model. Treatments A–M are specified in . B: Effects of different ratios and doses of β-ligands

    Techniques Used:

    Related Articles

    Cell Culture:

    Article Title: Feeding a Diet Enriched in Docosahexaenoic Acid to Lactating Dams Improves the Tolerance Response to Egg Protein in Suckled Pups
    Article Snippet: .. Briefly, immune cells (1.25 × 106 cells/mL) were cultured for 48 h without mitogen (unstimulated cells) or with concanavalin A (ConA, 2.5 µg/mL; MP Biomedicals, Montreal, Quebec, Canada), lipopolysaccharide (LPS, 100 µg/mL, Sigma), or ovalbumin (OVA, 100 µg/mL, Sigma). .. Commercial ELISA kits were used to measure the concentrations of IL-1β, IL-2, IL-6, IL-10, TNF-α, TGF-β, and IFN-γ according to the manufacturer’s instructions and as described previously [ ].

    Article Title: Statins accelerate the onset of collagen type II-induced arthritis in mice
    Article Snippet: .. LN cell suspensions were cultured at 106 cells/ml culture medium with 5 μg/ml Concanavalin A (MP Biomedicals, Irvine, CA, USA) in 96-well tissue culture plates (Nunc, Roskilde, Denmark) for 24 hours. .. Spleen cell suspensions were cultured at 106 cells/ml culture medium with 50 μg/ml CII or 5 μg/ml Concanavalin A in 96-well tissue culture plates (Nunc) for 72 hours.

    Article Title: The crystal structure of titanium dioxide nanoparticles influences immune activity in vitro and in vivo
    Article Snippet: .. LN cell suspensions were cultured at 106 cells/mL culture medium with 5 μg/mL Concanavalin A (MP Biomedicals, Irvine, CA, USA) in 96-well tissue culture plates (Nunc, Roskilde, Denmark) for 24 h. Spleen cell suspensions were cultured at 106 cells/mL culture medium with 1 mg/mL OVA in 96-well tissue culture plates (Nunc) for 120 h. Culture conditions were 37 °C in a humidified atmosphere containing 5% CO2 . .. Serum Ig and cytokine measurements OVA-specific IgE and OVA-specific IgG1 were measured using an ELISA (Cayman Chemicals, Sanbio, Uden, the Netherlands).

    Mouse Assay:

    Article Title: Impaired liver regeneration is associated with reduced cyclin B1 in natural killer T cell-deficient mice
    Article Snippet: .. ConA treatment and CD1d targeting Wild type or CD1d-/- mice were injected intravenously with either vehicle (50 mmol/L Tris, 150 mmol/L sodium chloride and 4 mmol/L calcium chloride) or with 10 mg/kg ConA (MP Biomedicals, Ohio, USA) 4 days before PH. .. Depletion of NKT cells was carried out by administration of anti-CD1d mAb, which can effectively block CD1d receptor [ ].

    Lysis:

    Article Title: A Model for Cell Wall Dissolution in Mating Yeast Cells: Polarized Secretion and Restricted Diffusion of Cell Wall Remodeling Enzymes Induces Local Dissolution
    Article Snippet: .. Concanavalin-A coated coverslip lysis assay Coverslips (VWR, PA) were coated in concanavalin A (MP Biomedicals, OH) in a protocol modified from Joglekar et al. (2008) . .. Briefly, coverslips were soaked in 1M NaOH for one hour at room temperature (25°C), rinsed five times with deionized, filtered water, and then incubated at room temperature for one hour in a solution of 10 mM Na2 HPO4 pH 6.0 (Fisher Biotech, MA) +1 mM CaCl2 +0.5 mg/mL concanavalin A. Coverslips were then rinsed five times with deionized, filtered water and air-dried over a 100°C heat block.

    Modification:

    Article Title: A Model for Cell Wall Dissolution in Mating Yeast Cells: Polarized Secretion and Restricted Diffusion of Cell Wall Remodeling Enzymes Induces Local Dissolution
    Article Snippet: .. Concanavalin-A coated coverslip lysis assay Coverslips (VWR, PA) were coated in concanavalin A (MP Biomedicals, OH) in a protocol modified from Joglekar et al. (2008) . .. Briefly, coverslips were soaked in 1M NaOH for one hour at room temperature (25°C), rinsed five times with deionized, filtered water, and then incubated at room temperature for one hour in a solution of 10 mM Na2 HPO4 pH 6.0 (Fisher Biotech, MA) +1 mM CaCl2 +0.5 mg/mL concanavalin A. Coverslips were then rinsed five times with deionized, filtered water and air-dried over a 100°C heat block.

    Staining:

    Article Title: Essential gene disruptions reveal complex relationships between phenotypic robustness, pleiotropy, and fitness
    Article Snippet: .. Cells were washed twice with PBS (200 μl/well) and then stained with a solution of 20 μg/ml FITC-conjugated concanavalin A (MP Biomedicals, 75 μl/well) for 1 h at room temperature in the dark. ..

    Injection:

    Article Title: Impaired liver regeneration is associated with reduced cyclin B1 in natural killer T cell-deficient mice
    Article Snippet: .. ConA treatment and CD1d targeting Wild type or CD1d-/- mice were injected intravenously with either vehicle (50 mmol/L Tris, 150 mmol/L sodium chloride and 4 mmol/L calcium chloride) or with 10 mg/kg ConA (MP Biomedicals, Ohio, USA) 4 days before PH. .. Depletion of NKT cells was carried out by administration of anti-CD1d mAb, which can effectively block CD1d receptor [ ].

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