sephadex  (Valiant)

 
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    Name:
    Sephadex G 25
    Description:
    Sephadex G 25
    Catalog Number:
    02195254-cf
    Price:
    10.0
    Category:
    Life Sciences Biochemicals
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    Structured Review

    Valiant sephadex
    Sephadex G 25
    https://www.bioz.com/result/sephadex/product/Valiant
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sephadex - by Bioz Stars, 2021-03
    86/100 stars

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    Filtration:

    Article Title: Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes
    Article Snippet: Bovine serum albumin was used as a standard and the results were quantified on a Multiskan FC (Thermo Scientific) microplate reader. .. For microinjection, proteins were desalted by Gel filtration using Sephadex G-25 (MP Biomedicals). .. In the case of NSF D1EQ, efficient desalting was achieved using a Microcon YM-30 centrifugal filter device (Millipore) according to the manufacturer’s instructions.

    other:

    Article Title: Highly Sensitive Immunochromatographic Identification of Tetracycline Antibiotics in Milk
    Article Snippet: Sephadex G-25 was obtained from MP Biomedicals (Santa Ana, CA).

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    Valiant sephadex g 50
    Electrophoresis of the various stages of DNase purification on a 4%–15% gradient polyacrylamide gel. ( 1 ) 250–10 kDa protein ladder; ( 2 ) crude DNase; ( 3 ) concentrated DNase; ( 4 ) Sephadex G-50 purified DNase; and ( 5 ) membrane purification following Sephadex G-50 column purification.
    Sephadex G 50, supplied by Valiant, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sephadex g 50/product/Valiant
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sephadex g 50 - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

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    Electrophoresis of the various stages of DNase purification on a 4%–15% gradient polyacrylamide gel. ( 1 ) 250–10 kDa protein ladder; ( 2 ) crude DNase; ( 3 ) concentrated DNase; ( 4 ) Sephadex G-50 purified DNase; and ( 5 ) membrane purification following Sephadex G-50 column purification.

    Journal: International Journal of Molecular Sciences

    Article Title: Purification of an Inducible DNase from a Thermophilic Fungus

    doi: 10.3390/ijms15011300

    Figure Lengend Snippet: Electrophoresis of the various stages of DNase purification on a 4%–15% gradient polyacrylamide gel. ( 1 ) 250–10 kDa protein ladder; ( 2 ) crude DNase; ( 3 ) concentrated DNase; ( 4 ) Sephadex G-50 purified DNase; and ( 5 ) membrane purification following Sephadex G-50 column purification.

    Article Snippet: Next, 1.0 mL of concentrated sample was passed through a Sephadex G-50 (MP Biomedicals Cat# ICN19558010; Solon, OH, USA) column.

    Techniques: Electrophoresis, Purification

    Purification profile of DNase activity and protein. Concentrated sample (1 mL) was passed through a Sephadex G-50 column at 2–5 °C. The DNase activity was measured using the acid soluble assay at 55 °C and protein was estimated by determining the absorbance at 280 nm.

    Journal: International Journal of Molecular Sciences

    Article Title: Purification of an Inducible DNase from a Thermophilic Fungus

    doi: 10.3390/ijms15011300

    Figure Lengend Snippet: Purification profile of DNase activity and protein. Concentrated sample (1 mL) was passed through a Sephadex G-50 column at 2–5 °C. The DNase activity was measured using the acid soluble assay at 55 °C and protein was estimated by determining the absorbance at 280 nm.

    Article Snippet: Next, 1.0 mL of concentrated sample was passed through a Sephadex G-50 (MP Biomedicals Cat# ICN19558010; Solon, OH, USA) column.

    Techniques: Purification, Activity Assay