tris hcl (Valiant)

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Name:

Tris hydrochloride reagent grade 99

Description:

Tris Hydrochloride

Catalog Number:

02103130-cf

Price:

10.0

Applications:

Biological buffer, Apoptosis

Category:

Life Sciences Biochemicals Ultra Pure Buffer Reagents

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Structured Review

Valiant tris hcl
Tris hydrochloride reagent grade 99

Tris Hydrochloride
https://www.bioz.com/result/tris hcl/product/Valiant
Average 94 stars, based on 49 article reviews
Price from $9.99 to $1999.99

tris hcl - by Bioz Stars, 2021-02

94/100 stars

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Images

DNA Extraction:

Article Title: Comparison of the gut microbial community between obese and lean peoples using 16S gene sequencing in a Japanese population
Article Snippet: .. DNA extraction DNA was extracted from fecal samples according to a previously described method. The fecal samples were suspended in a buffer containing 4 M guanidium thiocyanate, 100 mM Tris-HCl (pH 9.0) and 40 mM EDTA and beaten in the presence of zirconia beads using the FastPrep FP100A Instrument (MP Biomedicals, Irvine, CA). ..

High Performance Liquid Chromatography:

Article Title: Leishmania major possesses a unique HemG-type protoporphyrinogen IX oxidase
Article Snippet: .. HPLC cultures were centrifuged by 2500×g, and cells were mechanically disrupted with glass beads (100 μm) in 50 mM Tris–HCl pH 8.0 containing 2% (v/v) Tween 80 using FastPrep®-24 Instrument (MP Biomedicals). ..

Centrifugation:

Article Title: The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary β-mannans
Article Snippet: .. Cell pellet was collected by centrifugation (4500 × g , 10 min, 4 °C), resuspended in 50 mM Tris-HCl, 0.1% (v/v) Triton X-100, 200 mM NaCl, 1 mM dithiothreitol and disrupted by bead-beating using three 60 s cycles with a FastPrep24 (MP Biomedicals, CA). .. Proteins were precipitated with ice-cold trichloroacetic acid (TCA), final concentration of 10% (v/v), incubated on ice for 1 h, centrifuged (15,000 × g , 15 min, 4 °C) to pellet the precipitated proteins and washed with 300 μl ice-cold 0.01 M HCl in 90% acetone.

Article Title: Effect of Saccharomyces cerevisiae ret1-1 Mutation on Glycosylation and Localization of the Secretome
Article Snippet: .. The SDS-treated cell walls were pelleted by centrifugation at 1,500 × g for 5 min, washed with 1 mM phenylmethylsulfonyl fluoride 5 times, once with 50 mM Tris-HCl buffer (pH 8.0), and digested with 20 units/ml Quantazyme (Qbiogene, Montreal, Canada) in 0.2 ml reaction buffer (67 mM KH2 PO4 (pH 7.5) and 120 mM β-mercaptoethanol) at 30℃ for 6 h. The reaction mixture containing solubilized proteins was centrifuged at 15,000 × g for 10 min, and the supernatant was analyzed as β-1,3-glucanase-extracted cell wall proteins. .. Protein samples were subjected to SDS-PAGE using the method described by and electroblotted onto a polyvinylidene difluoride (PVDF) membrane in a solution of 25 mM Tris, 192 mM glycine, 20% methanol, and 0.05% SDS using Semiphore transfer units (Amersham).

Incubation:

Article Title: Functional interactions between Choline kinase ?, epidermal growth factor receptor (EGFR), and c-Src in breast cancer cell proliferations
Article Snippet: .. Aliquots of cell lysate were incubated in the reaction buffer containing 0.1M Tris HCl pH 8.0, 12 mM MgCl2 , 10 mM ATP, [methyl-14 C]-Choline chloride 0.25 mM (MP Biomedicals, Solon, OH), 1% NP-40 for 15 min at 37°C. .. Phosphocholine was isolated using AG 1-x8 Resin (Bio-Rad) as described in , and radioactivity was measured by scintillation counting.

other:

Article Title: Succession of Bifidobacterium longum strains in response to the changing early-life nutritional environment reveals specific adaptations to distinct dietary substrates
Article Snippet: Cells were resuspended in 50 mM Tris-HCl pH 8.4 and disrupted by bead-beating in three 60 s cycles using a FastPrep24 (MP Biomedicals, CA).

Article Title: A protein-protein interaction map of the TNF-induced NF-κB signal transduction pathway
Article Snippet: For every experiment, 100 μl MyOne Streptavidin T1 beads in suspension (10 mg/ml, Thermo Fisher Scientific, cat. no. 65601) were washed with 300 μl wash buffer containing 20 mM Tris-HCl (MP Biomedicals, cat. no. 02194558) pH 7.5 and 150 mM NaCl (Sigma, cat. no. S6191) and pre-loaded with 10 μl biotinylated anti-FLAG antibody (BioM2, Sigma, cat. no. F9291) in 500 μl wash buffer for 10 min at room temperature.

Polymerase Chain Reaction:

Article Title: Mitochondrial control region I and microsatellite analyses of endangered Philippine hornbill species (Aves; Bucerotidae) detect gene flow between island populations and genetic diversity loss
Article Snippet: .. The 37.5 μl PCR reaction volumes (buffer solution: 10mM Tris-HCl, pH 9.0, 50mM KCl, 1.5mM MgCl2 , 0.1% Triton X100, 0.2 mg/ml Bovine Serum Albumin (BSA)) were set up as follows: 6 μl dNTP Mixture (2.0 mM each), 1.2 μl of each primer (10 μM), 3 μl DNA template, 0.25 μl Taq polymerase (5U/μl, MP Biomedicals). ..