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melatonin elisa kit  (Valiant Co Ltd)
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Valiant Co Ltd melatonin elisa kit
The gut is a source <t>of</t> <t>melatonin</t> and levels of mesenteric melatonin and gut melatonin synthesizing enzymes are influenced by major themal injury as well as exogenous melatonin. A. In sham rats, <t>ELISA</t> melatonin measurements revealed that melatonin levels in mesenteric blood were around 9 times higher than those found in the general circulation (Far left bars). Contrariwise, plasma melatonin levels were profoundly increased in both mesenteric and general circulation in rats with a 3rd degree burn over 30% TBSA 3 days after thermal injury (TI, far right, p<0.01). Interestingly, these mesenteric and general circulation plasma melatonin levels were a very close match to circulating and mesenteric plasma melatonin levels found in sham rats 2 hours after exogenous melatonin (Sham+mel; 2mM, 1 ml/250 g b.wt., i.p.) injection. B. Gut AANAT and HIOMT mRNA expression levels were higher (^^) in thermally injured (TI) than sham control (*) ileum.C. AANAT mRNA expression in TI subjects was more suppressed by a higher (8 mM) than a lower (2 mM) dose of exogenous melatonin administered by IP injection. rtPCR products appear at 109 bp (AANAT), 260bp (HIOMT) and 222 bp (housekeeping GAPDH). All measurements were performed on blood samples and tissues obtained at ~ZT 8 of a 12:12 light cycle at resting conditions with free access to chow feed.
Melatonin Elisa Kit, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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melatonin elisa kit - by Bioz Stars, 2026-02
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The gut is a source of melatonin and levels of mesenteric melatonin and gut melatonin synthesizing enzymes are influenced by major themal injury as well as exogenous melatonin. A. In sham rats, ELISA melatonin measurements revealed that melatonin levels in mesenteric blood were around 9 times higher than those found in the general circulation (Far left bars). Contrariwise, plasma melatonin levels were profoundly increased in both mesenteric and general circulation in rats with a 3rd degree burn over 30% TBSA 3 days after thermal injury (TI, far right, p<0.01). Interestingly, these mesenteric and general circulation plasma melatonin levels were a very close match to circulating and mesenteric plasma melatonin levels found in sham rats 2 hours after exogenous melatonin (Sham+mel; 2mM, 1 ml/250 g b.wt., i.p.) injection. B. Gut AANAT and HIOMT mRNA expression levels were higher (^^) in thermally injured (TI) than sham control (*) ileum.C. AANAT mRNA expression in TI subjects was more suppressed by a higher (8 mM) than a lower (2 mM) dose of exogenous melatonin administered by IP injection. rtPCR products appear at 109 bp (AANAT), 260bp (HIOMT) and 222 bp (housekeeping GAPDH). All measurements were performed on blood samples and tissues obtained at ~ZT 8 of a 12:12 light cycle at resting conditions with free access to chow feed.

Journal: International Journal of Biological Sciences

Article Title: Melatonin Plays a Protective Role in Postburn Rodent Gut Pathophysiology

doi:

Figure Lengend Snippet: The gut is a source of melatonin and levels of mesenteric melatonin and gut melatonin synthesizing enzymes are influenced by major themal injury as well as exogenous melatonin. A. In sham rats, ELISA melatonin measurements revealed that melatonin levels in mesenteric blood were around 9 times higher than those found in the general circulation (Far left bars). Contrariwise, plasma melatonin levels were profoundly increased in both mesenteric and general circulation in rats with a 3rd degree burn over 30% TBSA 3 days after thermal injury (TI, far right, p<0.01). Interestingly, these mesenteric and general circulation plasma melatonin levels were a very close match to circulating and mesenteric plasma melatonin levels found in sham rats 2 hours after exogenous melatonin (Sham+mel; 2mM, 1 ml/250 g b.wt., i.p.) injection. B. Gut AANAT and HIOMT mRNA expression levels were higher (^^) in thermally injured (TI) than sham control (*) ileum.C. AANAT mRNA expression in TI subjects was more suppressed by a higher (8 mM) than a lower (2 mM) dose of exogenous melatonin administered by IP injection. rtPCR products appear at 109 bp (AANAT), 260bp (HIOMT) and 222 bp (housekeeping GAPDH). All measurements were performed on blood samples and tissues obtained at ~ZT 8 of a 12:12 light cycle at resting conditions with free access to chow feed.

Article Snippet: Plasma melatonin levels were assessed utilizing a commercial melatonin ELISA kit (MP Biomedicals' Inc., Irvin CA).

Techniques: Enzyme-linked Immunosorbent Assay, Injection, Expressing, Reverse Transcription Polymerase Chain Reaction