Journal: International journal of translational medicine (Basel, Switzerland)

Article Title: Comparison of the Differing Impacts of Lowered N-Acetylglucosaminyltransferase-Ia/b Activity on Motor and Sensory Function in Zebrafish

doi: 10.3390/ijtm5030036

Figure Lengend Snippet: Increased levels of oligomannose-type N-glycans in adult mgat1a −/− zebrafish. Lectin blots of tissue homogenates from mgat1a+/+ , mgat1a −/− and mgat1a+/ − fish, as indicated ( A – F ). Electrophoresed proteins were probed with GNL (left panels). Protein gels stained with Coomassie blue (CB) (right panels) to demonstrate equal protein loads among the samples. Quantification of the oligomannosylated proteins from swim bladder (SB, n = 3), skin (S, n = 3), heart (H, n = 3), skeletal muscle (SM, n = 3), spinal cord (SC, n = 3), and brain (B, n = 4) ( G ). Numbers adjacent to the blots denote protein markers (in kDa). Data is mean ± S.E. and compared via Student’s t -test (* p at 0.05).

Article Snippet: Post migration, gels were either stained with Coomassie ® Brilliant Blue (MP Biomedical, Solon, OH, USA) or utilized to transfer proteins to a PVDF membrane (Whatman, Dassel, Germany) for lectin blotting, following previously established protocols [ ].

Techniques: Staining

Journal: International journal of translational medicine (Basel, Switzerland)

Article Title: Comparison of the Differing Impacts of Lowered N-Acetylglucosaminyltransferase-Ia/b Activity on Motor and Sensory Function in Zebrafish

doi: 10.3390/ijtm5030036

Figure Lengend Snippet: Comparison of oligomannosylated protein in mutant and Wt AB fish strains. Lectin blot and Coomassie blue-stained gels of embryo (48 hpf) ( A ), and tissues from adult strains ( C – J ). Quantification of the oligomannosylated proteins from embryos ( n = 5; Panel ( B )), and adult tissues, including swim bladder (SB, n = 3), skin (S, n = 3), heart (H, n = 3), skeletal muscle (SM, n = 3), spinal cord (SC, n = 3), brain (B, n = 4), brain regions 1 (R1, n = 5) and 2 (R2, n = 3) (Panel ( K )). Brain regions 1 includes olfactory bulb, telencephalon, habenula, and about half of optic tectum, while brain region 2 includes about half of the optic tectum, cerebellum, and medulla. Numbers adjacent to the blots signify protein markers (in kDa). Data were compared Student’s t-test (* p at 0.02).

Article Snippet: Post migration, gels were either stained with Coomassie ® Brilliant Blue (MP Biomedical, Solon, OH, USA) or utilized to transfer proteins to a PVDF membrane (Whatman, Dassel, Germany) for lectin blotting, following previously established protocols [ ].

Techniques: Comparison, Mutagenesis, Staining