ponceau s  (Valiant)


Bioz Verified Symbol Valiant is a verified supplier
Bioz Manufacturer Symbol Valiant manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Name:
    Ponceau S
    Description:
    Ponceau S
    Catalog Number:
    02190644-cf
    Price:
    10.0
    Applications:
    Protein stain for electrophoresis
    Category:
    Life Sciences Biochemicals Stains Dyes Protein Dyes
    Buy from Supplier


    Structured Review

    Valiant ponceau s
    Ponceau S
    Ponceau S
    https://www.bioz.com/result/ponceau s/product/Valiant
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ponceau s - by Bioz Stars, 2021-02
    92/100 stars

    Images

    1) Product Images from "MTH1, an Oxidized Purine Nucleoside Triphosphatase, Suppresses the Accumulation of Oxidative Damage of Nucleic Acids in the Hippocampal Microglia during Kainate-Induced Excitotoxicity"

    Article Title: MTH1, an Oxidized Purine Nucleoside Triphosphatase, Suppresses the Accumulation of Oxidative Damage of Nucleic Acids in the Hippocampal Microglia during Kainate-Induced Excitotoxicity

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.4948-05.2006

    Increased expression of MTH1 protein in the hippocampus under the excitotoxicity. A , Western blotting analysis of MTH1 protein. Whole-cell extracts prepared from the mouse hippocampus prepared at 12, 24, 72 h, and 1 week after kainate administration were subjected to a Western blotting analysis with anti-MTH1 and anti-β-actin. The Western blotting results are shown in the top (MTH1) and middle (β-actin) panels. The bottom panel is a Ponceau S-stained filter before incubation with the antibodies to confirm sample loading. Extracts from MTH1-null mouse (KO) and recombinant mouse MTH1 (mMTH1) were also subjected to Western blotting. The arrowhead indicates mMTH1. B , The increased expression of MTH1 protein in the hippocampus after kainate administration. The amount of MTH1 protein determined by Western blotting was normalized by that of β-actin at each time point, and the mean value of the relative amount of MTH1 protein at each time point to that of the control is shown in the bar graph.  n  = 2 mice per group.
    Figure Legend Snippet: Increased expression of MTH1 protein in the hippocampus under the excitotoxicity. A , Western blotting analysis of MTH1 protein. Whole-cell extracts prepared from the mouse hippocampus prepared at 12, 24, 72 h, and 1 week after kainate administration were subjected to a Western blotting analysis with anti-MTH1 and anti-β-actin. The Western blotting results are shown in the top (MTH1) and middle (β-actin) panels. The bottom panel is a Ponceau S-stained filter before incubation with the antibodies to confirm sample loading. Extracts from MTH1-null mouse (KO) and recombinant mouse MTH1 (mMTH1) were also subjected to Western blotting. The arrowhead indicates mMTH1. B , The increased expression of MTH1 protein in the hippocampus after kainate administration. The amount of MTH1 protein determined by Western blotting was normalized by that of β-actin at each time point, and the mean value of the relative amount of MTH1 protein at each time point to that of the control is shown in the bar graph. n = 2 mice per group.

    Techniques Used: Expressing, Western Blot, Staining, Incubation, Recombinant, Mouse Assay

    2) Product Images from "Dissection of Cell Death Induction by Wheat Stem Rust Resistance Protein Sr35 and Its Matching Effector AvrSr35"

    Article Title: Dissection of Cell Death Induction by Wheat Stem Rust Resistance Protein Sr35 and Its Matching Effector AvrSr35

    Journal: Molecular plant-microbe interactions : MPMI

    doi: 10.1094/MPMI-08-19-0216-R

    AvrSr35 recognition by Sr35 in barley. A, Model of AvrSr35–SP+HA (no signal peptide and C-terminal hemagglutinin [HA] tag) construct. B, Transient expression of Sr35 and AvrSr35–SP+HA constructs in barley. Top panel: macroscopic cell death in Manchuria barley leaves 7 days postinfiltration with Agrobacterium tumefaciens carrying Sr35 and AvrSr35–SP+HA constructs. Ratios represent the number of the 20 leaves transformed with each construct that showed the same result as the presented images. Lower panel: macroscopic cell death in Nicotiana benthamiana leaves 72 h postinfiltration with the same Agrobacterium culture. Leaf images were cropped to save space. The experiment was repeated twice with similar results. C, HA-horseradish peroxidase Western Blot (upper panel) showing AvrSr35–SP+HA expressed at the expected size in N. benthamiana . ev = empty vector. The blot was also stained with Ponceau S (lower panel) to reveal bands corresponding to the Rubisco large subunit protein as a loading control.
    Figure Legend Snippet: AvrSr35 recognition by Sr35 in barley. A, Model of AvrSr35–SP+HA (no signal peptide and C-terminal hemagglutinin [HA] tag) construct. B, Transient expression of Sr35 and AvrSr35–SP+HA constructs in barley. Top panel: macroscopic cell death in Manchuria barley leaves 7 days postinfiltration with Agrobacterium tumefaciens carrying Sr35 and AvrSr35–SP+HA constructs. Ratios represent the number of the 20 leaves transformed with each construct that showed the same result as the presented images. Lower panel: macroscopic cell death in Nicotiana benthamiana leaves 72 h postinfiltration with the same Agrobacterium culture. Leaf images were cropped to save space. The experiment was repeated twice with similar results. C, HA-horseradish peroxidase Western Blot (upper panel) showing AvrSr35–SP+HA expressed at the expected size in N. benthamiana . ev = empty vector. The blot was also stained with Ponceau S (lower panel) to reveal bands corresponding to the Rubisco large subunit protein as a loading control.

    Techniques Used: Construct, Expressing, Transformation Assay, Western Blot, Plasmid Preparation, Staining

    AvrSr35 and Sr35 with a C-terminal green fluorescent protein (GFP) tag trigger a strong cell-death response. A, Schematic diagram of Sr35 and AvrSr35 constructs. B, Macroscopic cell death in Nicotiana benthamiana leaves 48 h postinfiltration (hpi) with Agrobacterium tumefaciens (+ indicates coinfiltration). Avr refers to the AvrSr35–SP+mRFP construct (no signal peptide and monomeric red fluorescent protein [mRFP] C-terminal tag). Avr+Avr coinfiltration control corresponds to AvrSr35 infiltrated at double the optical density. C, Electrolyte leakage for all constructs was monitored from 20 to 50 hpi. Error bars represent standard error based on four biological replicates per construct. Different letters represent significantly different groups of means, based on Tukey’s honestly significant difference test (α = 0.01) performed at the last timepoint (statistical analyses in Supplementary Table S6 ). D, GFP-HRP (horseradish peroxidase) Western blot (upper panel) showing that all constructs except Sr35 D503V-GFP expressed proteins of the expected sizes. Sr35 D503V-GFP was detected by immunoprecipitation ( Supplementary Fig. S2 ). The blot shown below is the same blot stained with Ponceau S to reveal bands corresponding to the Rubisco large subunit protein as a loading control. DsRed and mouse-HRP Western blot (lower panel) revealed AvrSr35 in all coinfiltrated samples except Avr+Sr35 D503V-GFP, likely due to rapid cell death. The blot shown below is the same blot stained with Ponceau S to reveal bands corresponding to the Rubisco large subunit protein as loading control.
    Figure Legend Snippet: AvrSr35 and Sr35 with a C-terminal green fluorescent protein (GFP) tag trigger a strong cell-death response. A, Schematic diagram of Sr35 and AvrSr35 constructs. B, Macroscopic cell death in Nicotiana benthamiana leaves 48 h postinfiltration (hpi) with Agrobacterium tumefaciens (+ indicates coinfiltration). Avr refers to the AvrSr35–SP+mRFP construct (no signal peptide and monomeric red fluorescent protein [mRFP] C-terminal tag). Avr+Avr coinfiltration control corresponds to AvrSr35 infiltrated at double the optical density. C, Electrolyte leakage for all constructs was monitored from 20 to 50 hpi. Error bars represent standard error based on four biological replicates per construct. Different letters represent significantly different groups of means, based on Tukey’s honestly significant difference test (α = 0.01) performed at the last timepoint (statistical analyses in Supplementary Table S6 ). D, GFP-HRP (horseradish peroxidase) Western blot (upper panel) showing that all constructs except Sr35 D503V-GFP expressed proteins of the expected sizes. Sr35 D503V-GFP was detected by immunoprecipitation ( Supplementary Fig. S2 ). The blot shown below is the same blot stained with Ponceau S to reveal bands corresponding to the Rubisco large subunit protein as a loading control. DsRed and mouse-HRP Western blot (lower panel) revealed AvrSr35 in all coinfiltrated samples except Avr+Sr35 D503V-GFP, likely due to rapid cell death. The blot shown below is the same blot stained with Ponceau S to reveal bands corresponding to the Rubisco large subunit protein as loading control.

    Techniques Used: Construct, Western Blot, Immunoprecipitation, Staining

    Truncation variants of Sr35 are not able to induce cell death. A, Model of Sr35 protein with amino acid numbers used to delineate the boundaries of domains and motifs listed below. CC = coiled-coil, NB = nucleotide binding, NB-ARC = nucleotide binding adaptor, and LRR = leucine-rich repeat. Sr35 wild-type (WT) protein and its truncated variants are shown below the model. B, Macroscopic cell death in Nicotiana benthamiana leaves 48 h postinfiltration (hpi) with Sr35 and its truncated variants. All proteins were C-terminally tagged with green fluorescent protein (GFP); ev refers to empty vector. C, Electrolyte leakage was monitored from 20 to 50 hpi. Error bars correspond to standard error based on four biological replicates per construct. Different letters represent significantly different groups of means, based on Tukey’s honestly significant difference test (α = 0.01) performed at the last timepoint (statistical analyses in Supplementary Table S3 ). D, GFP-horseradish peroxidase Western blot showing proteins expressed at expected sizes for all constructs. GFP cleavage (*) was observed in CC, NB, and CC-NB constructs. The lower panel is the same blot stained with Ponceau S to reveal bands corresponding to the Rubisco large subunit protein as loading control.
    Figure Legend Snippet: Truncation variants of Sr35 are not able to induce cell death. A, Model of Sr35 protein with amino acid numbers used to delineate the boundaries of domains and motifs listed below. CC = coiled-coil, NB = nucleotide binding, NB-ARC = nucleotide binding adaptor, and LRR = leucine-rich repeat. Sr35 wild-type (WT) protein and its truncated variants are shown below the model. B, Macroscopic cell death in Nicotiana benthamiana leaves 48 h postinfiltration (hpi) with Sr35 and its truncated variants. All proteins were C-terminally tagged with green fluorescent protein (GFP); ev refers to empty vector. C, Electrolyte leakage was monitored from 20 to 50 hpi. Error bars correspond to standard error based on four biological replicates per construct. Different letters represent significantly different groups of means, based on Tukey’s honestly significant difference test (α = 0.01) performed at the last timepoint (statistical analyses in Supplementary Table S3 ). D, GFP-horseradish peroxidase Western blot showing proteins expressed at expected sizes for all constructs. GFP cleavage (*) was observed in CC, NB, and CC-NB constructs. The lower panel is the same blot stained with Ponceau S to reveal bands corresponding to the Rubisco large subunit protein as loading control.

    Techniques Used: Binding Assay, Plasmid Preparation, Construct, Western Blot, Staining

    Sr35 induces cell death when overexpressed in Nicotiana benthamiana . A, Schematic diagram of green fluorescent protein (GFP) fused to the N (GFP-Sr35) and C (Sr35-GFP) terminus of Sr35 with and without the autoactive mutation (D503V). B, Macroscopic cell death observed 48 h postinfiltration (hpi) with Agrobacterium tumefaciens ; ev = empty vector. C, Electrolyte leakage 15 to 45 hpi. Error bars represent standard error based on four biological replicates per construct. Different letters indicate significantly different groups of means based on Tukey’s honestly significant difference test (α= 0.01) performed at 45 hpi (statistical analyses in Supplementary Table S2 ). D, Western blots of protein extracts from N. benthamiana 24 hpi, analyzed using an anti-GFP-horseradish peroxidase antibody. No protein was observed here for Sr35 D503V-GFP, but it was detected after immunoprecipitation ( Supplementary Fig. S2 ). The lower panel is the same blot stained with Ponceau S to reveal the Rubisco large subunit protein used as loading control.
    Figure Legend Snippet: Sr35 induces cell death when overexpressed in Nicotiana benthamiana . A, Schematic diagram of green fluorescent protein (GFP) fused to the N (GFP-Sr35) and C (Sr35-GFP) terminus of Sr35 with and without the autoactive mutation (D503V). B, Macroscopic cell death observed 48 h postinfiltration (hpi) with Agrobacterium tumefaciens ; ev = empty vector. C, Electrolyte leakage 15 to 45 hpi. Error bars represent standard error based on four biological replicates per construct. Different letters indicate significantly different groups of means based on Tukey’s honestly significant difference test (α= 0.01) performed at 45 hpi (statistical analyses in Supplementary Table S2 ). D, Western blots of protein extracts from N. benthamiana 24 hpi, analyzed using an anti-GFP-horseradish peroxidase antibody. No protein was observed here for Sr35 D503V-GFP, but it was detected after immunoprecipitation ( Supplementary Fig. S2 ). The lower panel is the same blot stained with Ponceau S to reveal the Rubisco large subunit protein used as loading control.

    Techniques Used: Mutagenesis, Plasmid Preparation, Construct, Western Blot, Immunoprecipitation, Staining

    Sr35 coiled-coil nucleotide binding adaptor (CC-NB-ARC) D503V induces strong cell death. A, Model of Sr35 protein with the amino acid numbers used to delineate the boundaries of domains and motifs listed below. NB = nucleotide binding and LRR = leucine-rich repeat. The D503Vautoactive mutation is marked above the model. Truncation variants used in this experiment are shown below. B, Macroscopic cell death in Nicotiana benthamiana leaves 48 h postinfiltration (hpi) with Agrobacterium tumefaciens ; ev refers to empty vector construct. C, Electrolyte leakage for all constructs was monitored 20 to 50 hpi. Error bars represent standard error based on four biological replicates per construct. Different letters represent significantly different groups of means, based on Tukey’s honestly significant difference test (α = 0.01) performed at the last timepoint (statistical analyses in Supplementary Table S4 ). D, Green fluorescent protein (GFP) horseradish peroxidase Western blot showing that all constructs except Sr35 D503V-GFP expressed proteins of the expected sizes. Sr35 D503V-GFP was detected by immunoprecipitation ( Supplementary Fig. S2 ). Relatively weak protein expression was noted for all constructs with the D503V autoactive mutation. The lower panel is the same blot stained with Ponceau S to reveal bands corresponding to the Rubisco large subunit protein as loading control.
    Figure Legend Snippet: Sr35 coiled-coil nucleotide binding adaptor (CC-NB-ARC) D503V induces strong cell death. A, Model of Sr35 protein with the amino acid numbers used to delineate the boundaries of domains and motifs listed below. NB = nucleotide binding and LRR = leucine-rich repeat. The D503Vautoactive mutation is marked above the model. Truncation variants used in this experiment are shown below. B, Macroscopic cell death in Nicotiana benthamiana leaves 48 h postinfiltration (hpi) with Agrobacterium tumefaciens ; ev refers to empty vector construct. C, Electrolyte leakage for all constructs was monitored 20 to 50 hpi. Error bars represent standard error based on four biological replicates per construct. Different letters represent significantly different groups of means, based on Tukey’s honestly significant difference test (α = 0.01) performed at the last timepoint (statistical analyses in Supplementary Table S4 ). D, Green fluorescent protein (GFP) horseradish peroxidase Western blot showing that all constructs except Sr35 D503V-GFP expressed proteins of the expected sizes. Sr35 D503V-GFP was detected by immunoprecipitation ( Supplementary Fig. S2 ). Relatively weak protein expression was noted for all constructs with the D503V autoactive mutation. The lower panel is the same blot stained with Ponceau S to reveal bands corresponding to the Rubisco large subunit protein as loading control.

    Techniques Used: Binding Assay, Mutagenesis, Plasmid Preparation, Construct, Western Blot, Immunoprecipitation, Expressing, Staining

    Related Articles

    Staining:

    Article Title: Voltage-dependent-anion-channels (VDACs) in Arabidopsis have a dual localization in the cell but show a distinct role in mitochondria.
    Article Snippet: .. In mammals, the Voltage-dependent anion channels (VDACs) are predominant proteins of the outer mitochondrial membrane (OMM) where they contribute to the exchange of small metabolites essential for respiration. .. In mammals, the Voltage-dependent anion channels (VDACs) are predominant proteins of the outer mitochondrial membrane (OMM) where they contribute to the exchange of small metabolites essential for respiration.

    Article Title: Dissection of Cell Death Induction by Wheat Stem Rust Resistance Protein Sr35 and Its Matching Effector AvrSr35
    Article Snippet: .. Blots were stained with a Ponceau S and acetic acid solution (0.1% [wt/vol] Ponceau S [MP Biomedicals] and 5% [vol/vol] acetic acid) for 5 min. Blots were then rinsed with distilled water until bands were clearly visible and background was appropriately reduced. .. The stained blots were imaged using a ChemiDoc (BioRad).

    Incubation:

    Article Title: MTH1, an Oxidized Purine Nucleoside Triphosphatase, Suppresses the Accumulation of Oxidative Damage of Nucleic Acids in the Hippocampal Microglia during Kainate-Induced Excitotoxicity
    Article Snippet: .. After blotting, the membranes were incubated with 0.5% Ponceau S (MP Biomedicals, Ecshwege, Germany) to quantitate the amount of protein on the membranes. .. Recombinant mouse MTH1 (mMTH1) expressed in E. coli BL21T cells carrying pET3a:mMTH1 was used as a standard.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • N/A
    Erythrosin B Sodium Salt
      Buy from Supplier

    Image Search Results