nuclease p1  (Valiant)

 
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    Name:
    Nuclease P1 1 vial
    Description:
    Nuclease P1
    Catalog Number:
    0218055501
    Price:
    847.2
    Category:
    Life Sciences Biochemicals Proteins and Derivatives Enzymes
    Applications:
    Endonuclease
    Size:
    1 vial
    Buy from Supplier


    Structured Review

    Valiant nuclease p1
    Nuclease P1 1 vial
    Nuclease P1
    https://www.bioz.com/result/nuclease p1/product/Valiant
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nuclease p1 - by Bioz Stars, 2021-04
    92/100 stars

    Images

    1) Product Images from "5-Fluorouracil Treatment Alters the Efficiency of Translational Recoding"

    Article Title: 5-Fluorouracil Treatment Alters the Efficiency of Translational Recoding

    Journal: Genes

    doi: 10.3390/genes8110295

    Ribosomal RNA (rRNA) pseudouridine levels as well as rRNA levels in SW-480 cells treated with uracil or 5-FU. ( A ) 18S and 28S rRNA were gel-purified from [ 32 P]-orthophosphate pulsed SW-480 cells cultured in medium containing uracil (left panel) or 5-FU (right panel), and subjected to nuclease P1 digestion and 2-dimensional thin layer chromatography (2D-TLC). Spots corresponding to adenosine (pA), cytosine (pC), guanosine (pG), uridine (pU), and pseudouridine (pΨ) are labeled. For uracil-treated cells (left panel), the pU/pΨ ratio is ~5%; for 5-FU-treated cell (right), the pU/pΨ ratio is ~1%. ( B ) Total RNA from 5-FU- and uracil-treated cells was resolved by electrophoresis, and photographed (top). As a loading control, U2 small nuclear RNA (snRNA) was detected by Northern blotting (bottom). ( C ) Relative levels of 18S and 28S from 5-FU- and uracil-treated cells were quantified.
    Figure Legend Snippet: Ribosomal RNA (rRNA) pseudouridine levels as well as rRNA levels in SW-480 cells treated with uracil or 5-FU. ( A ) 18S and 28S rRNA were gel-purified from [ 32 P]-orthophosphate pulsed SW-480 cells cultured in medium containing uracil (left panel) or 5-FU (right panel), and subjected to nuclease P1 digestion and 2-dimensional thin layer chromatography (2D-TLC). Spots corresponding to adenosine (pA), cytosine (pC), guanosine (pG), uridine (pU), and pseudouridine (pΨ) are labeled. For uracil-treated cells (left panel), the pU/pΨ ratio is ~5%; for 5-FU-treated cell (right), the pU/pΨ ratio is ~1%. ( B ) Total RNA from 5-FU- and uracil-treated cells was resolved by electrophoresis, and photographed (top). As a loading control, U2 small nuclear RNA (snRNA) was detected by Northern blotting (bottom). ( C ) Relative levels of 18S and 28S from 5-FU- and uracil-treated cells were quantified.

    Techniques Used: Purification, Cell Culture, Thin Layer Chromatography, Labeling, Electrophoresis, Northern Blot

    Related Articles

    Filtration:

    Article Title: Autophagy and leucine promote chronological longevity and respiration proficiency during calorie restriction in yeast
    Article Snippet: Levels of 8-oxo-guanosine and 8-oxo-deoxyguanosine were quantified using an HPLC method ( ). .. Briefly, nucleic acid pellets were dissolved in 30 µM deferoxamine methanesulfonate and hydrolyzed using 4 units of nuclease P1 (MP Biomedicals, Solon, OH) and 5 units of alkaline phosphatase (Sigma-Aldrich, St. Louis, MO) in 30 mM sodium acetate, 20 µM ZnCl2 , pH 5.3 at 50°C for 60 min. After filtration to remove enzymes, nucleosides were separated using HPLC. ..

    High Performance Liquid Chromatography:

    Article Title: Autophagy and leucine promote chronological longevity and respiration proficiency during calorie restriction in yeast
    Article Snippet: Levels of 8-oxo-guanosine and 8-oxo-deoxyguanosine were quantified using an HPLC method ( ). .. Briefly, nucleic acid pellets were dissolved in 30 µM deferoxamine methanesulfonate and hydrolyzed using 4 units of nuclease P1 (MP Biomedicals, Solon, OH) and 5 units of alkaline phosphatase (Sigma-Aldrich, St. Louis, MO) in 30 mM sodium acetate, 20 µM ZnCl2 , pH 5.3 at 50°C for 60 min. After filtration to remove enzymes, nucleosides were separated using HPLC. ..

    Article Title: tRNAHis 5-methylcytidine levels increase in response to several growth arrest conditions in Saccharomyces cerevisiae
    Article Snippet: The resulting tRNA was desalted and concentrated using Amicon Ultra 4 10,000 MWCO columns (Millipore). .. tRNAs (1.25 μg) were digested at 37°C for at least 2 h using 0.5 μg P1 nuclease (MP Biomedicals) in a buffer containing 20 mM NaOAc pH 5.2 and 0.2 mM ZnCl2 and then treated with calf intestinal phosphatase (Roche) for at least 1 h. Nucleosides were resolved by reverse-phase HPLC essentially as described , and each nucleoside was identified and quantified as described previously ( ; ). .. RNA was extracted using the hot phenol extraction method ( ) and treated with RQ1 RNase-free DNase (Promega), followed by a phenol-chloroform extraction, two chloroform extractions, and ethanol precipitation.

    Incubation:

    Article Title: A Single Zinc Ion Is Sufficient for an Active Trypanosoma brucei tRNA Editing Deaminase *
    Article Snippet: .. The resulting pellet was suspended in 9 μl of 1× nuclease P1 buffer (as supplied with enzyme; 30 m m NaOAc, pH 5.3) and 1 μl of nuclease P1 (MPBiomedicals) and incubated overnight at 37 °C. .. Dried samples were suspended in 2.5 μl of H2 O, and 1 μl was spotted on a TLC cellulose plate (Merck).

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  • 92
    Valiant nuclease p1
    Ribosomal RNA (rRNA) pseudouridine levels as well as rRNA levels in SW-480 cells treated with uracil or 5-FU. ( A ) 18S and 28S rRNA were gel-purified from [ 32 P]-orthophosphate pulsed SW-480 cells cultured in medium containing uracil (left panel) or 5-FU (right panel), and subjected to <t>nuclease</t> P1 digestion and 2-dimensional thin layer chromatography (2D-TLC). Spots corresponding to adenosine (pA), cytosine (pC), guanosine (pG), uridine (pU), and pseudouridine (pΨ) are labeled. For uracil-treated cells (left panel), the pU/pΨ ratio is ~5%; for 5-FU-treated cell (right), the pU/pΨ ratio is ~1%. ( B ) Total RNA from 5-FU- and uracil-treated cells was resolved by electrophoresis, and photographed (top). As a loading control, U2 small nuclear RNA (snRNA) was detected by Northern blotting (bottom). ( C ) Relative levels of 18S and 28S from 5-FU- and uracil-treated cells were quantified.
    Nuclease P1, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nuclease p1/product/Valiant
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nuclease p1 - by Bioz Stars, 2021-04
    92/100 stars
      Buy from Supplier

    Image Search Results


    Ribosomal RNA (rRNA) pseudouridine levels as well as rRNA levels in SW-480 cells treated with uracil or 5-FU. ( A ) 18S and 28S rRNA were gel-purified from [ 32 P]-orthophosphate pulsed SW-480 cells cultured in medium containing uracil (left panel) or 5-FU (right panel), and subjected to nuclease P1 digestion and 2-dimensional thin layer chromatography (2D-TLC). Spots corresponding to adenosine (pA), cytosine (pC), guanosine (pG), uridine (pU), and pseudouridine (pΨ) are labeled. For uracil-treated cells (left panel), the pU/pΨ ratio is ~5%; for 5-FU-treated cell (right), the pU/pΨ ratio is ~1%. ( B ) Total RNA from 5-FU- and uracil-treated cells was resolved by electrophoresis, and photographed (top). As a loading control, U2 small nuclear RNA (snRNA) was detected by Northern blotting (bottom). ( C ) Relative levels of 18S and 28S from 5-FU- and uracil-treated cells were quantified.

    Journal: Genes

    Article Title: 5-Fluorouracil Treatment Alters the Efficiency of Translational Recoding

    doi: 10.3390/genes8110295

    Figure Lengend Snippet: Ribosomal RNA (rRNA) pseudouridine levels as well as rRNA levels in SW-480 cells treated with uracil or 5-FU. ( A ) 18S and 28S rRNA were gel-purified from [ 32 P]-orthophosphate pulsed SW-480 cells cultured in medium containing uracil (left panel) or 5-FU (right panel), and subjected to nuclease P1 digestion and 2-dimensional thin layer chromatography (2D-TLC). Spots corresponding to adenosine (pA), cytosine (pC), guanosine (pG), uridine (pU), and pseudouridine (pΨ) are labeled. For uracil-treated cells (left panel), the pU/pΨ ratio is ~5%; for 5-FU-treated cell (right), the pU/pΨ ratio is ~1%. ( B ) Total RNA from 5-FU- and uracil-treated cells was resolved by electrophoresis, and photographed (top). As a loading control, U2 small nuclear RNA (snRNA) was detected by Northern blotting (bottom). ( C ) Relative levels of 18S and 28S from 5-FU- and uracil-treated cells were quantified.

    Article Snippet: The RNA pellet was resuspended in 10 μL of 50 mM NH4 acetate (pH 5.3) containing 0.2 μg of nuclease P1 to (MP Biomedicals, cat. No. 195352, Solon, OH, USA).

    Techniques: Purification, Cell Culture, Thin Layer Chromatography, Labeling, Electrophoresis, Northern Blot