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Representative autoradiographic profiles of AA–DNA adducts detected by <t>the</t> <t>nuclease</t> <t>P1</t> enrichment version of the 32P-postlabeling assay. Electronic autoradiography was performed for 5–10 min. Ori-gins in the bottom left corners were cut off before exposure. (A) Liver control; (B) AA-treated liver; (C) kidney control; (D) AA-treated kid-ney; spot (1) dA–AAI; spot (2) dG–AAI; spot (3) dA–AAII.
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Representative autoradiographic profiles of AA–DNA adducts detected by <t>the</t> <t>nuclease</t> <t>P1</t> enrichment version of the 32P-postlabeling assay. Electronic autoradiography was performed for 5–10 min. Ori-gins in the bottom left corners were cut off before exposure. (A) Liver control; (B) AA-treated liver; (C) kidney control; (D) AA-treated kid-ney; spot (1) dA–AAI; spot (2) dG–AAI; spot (3) dA–AAII.
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Representative autoradiographic profiles of AA–DNA adducts detected by the nuclease P1 enrichment version of the 32P-postlabeling assay. Electronic autoradiography was performed for 5–10 min. Ori-gins in the bottom left corners were cut off before exposure. (A) Liver control; (B) AA-treated liver; (C) kidney control; (D) AA-treated kid-ney; spot (1) dA–AAI; spot (2) dG–AAI; spot (3) dA–AAII.

Journal: Mutation research

Article Title: DNA adduct formation and mutation induction by aristolochic acid in rat kidney and liver

doi: 10.1016/j.mrfmmm.2006.08.004

Figure Lengend Snippet: Representative autoradiographic profiles of AA–DNA adducts detected by the nuclease P1 enrichment version of the 32P-postlabeling assay. Electronic autoradiography was performed for 5–10 min. Ori-gins in the bottom left corners were cut off before exposure. (A) Liver control; (B) AA-treated liver; (C) kidney control; (D) AA-treated kid-ney; spot (1) dA–AAI; spot (2) dG–AAI; spot (3) dA–AAII.

Article Snippet: For nuclease P1 enrichment, the digests were incubated with 4 μg nuclease P1 (MP Biomedicals, London, UK) in 3 μl of a buffer containing 0.8 M sodium acetate (pH 5.0) and 2 mM zinc chloride for 30 min at 37 °C.

Techniques: Postlabeling Assay, Autoradiography