brefeldin a  (Valiant)


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    Name:
    Brefeldin A
    Description:
    Brefeldin A
    Catalog Number:
    02159027-cf
    Price:
    10.0
    Applications:
    Brefeldin A blocks protein transportation into post-Golgi compartments.
    Category:
    Life Sciences Biochemicals Antibiotics
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    Structured Review

    Valiant brefeldin a
    Brefeldin A
    Brefeldin A
    https://www.bioz.com/result/brefeldin a/product/Valiant
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    brefeldin a - by Bioz Stars, 2021-02
    93/100 stars

    Images

    1) Product Images from "Vascular smooth muscle cell-derived adiponectin: a paracrine regulator of contractile phenotype"

    Article Title: Vascular smooth muscle cell-derived adiponectin: a paracrine regulator of contractile phenotype

    Journal: Journal of Molecular and Cellular Cardiology

    doi: 10.1016/j.yjmcc.2011.09.008

    Adiponectin is synthesized in VSMC (A) Upper panel: VSMC from WT and adiponectin knock-out mice were treated with 100 ng/mL brefeldin A for 4 hours to arrest transport from ER to golgi to facilitiate visualization of ER proteins. Confocal microscopy was performed to show adiponectin (green), F-actin (red) and nuclei (DAPI, blue) staining, and multiple WT fields are shown. Middle panel: WT mouse VSMC treated with 100 ng/mL brefeldin A for 4 hours were stained for adiponectin (green), Calnexin (an ER marker, red), and DAPI (blue). Bottom panel: WT mouse VSMC (without brefeldin A treatment) were stained for adiponectin (green), NogoB (an ER protein, red), and DAPI (blue). The merged image (co-localization) is at right. (B) VSMC isolated from SM-GFP mice were stained for adiponectin, SM-alpha-actin, h-Caldesmon (blue, Cy5 secondary antibody), or fluorescent secondary antibodies only, and subjected to confocal microscopy analysis. Transgelin-lineage VSMC are green, non-SMC are red (tomato). Nuclei are stained with DAPI. No tomato red staining was detected.
    Figure Legend Snippet: Adiponectin is synthesized in VSMC (A) Upper panel: VSMC from WT and adiponectin knock-out mice were treated with 100 ng/mL brefeldin A for 4 hours to arrest transport from ER to golgi to facilitiate visualization of ER proteins. Confocal microscopy was performed to show adiponectin (green), F-actin (red) and nuclei (DAPI, blue) staining, and multiple WT fields are shown. Middle panel: WT mouse VSMC treated with 100 ng/mL brefeldin A for 4 hours were stained for adiponectin (green), Calnexin (an ER marker, red), and DAPI (blue). Bottom panel: WT mouse VSMC (without brefeldin A treatment) were stained for adiponectin (green), NogoB (an ER protein, red), and DAPI (blue). The merged image (co-localization) is at right. (B) VSMC isolated from SM-GFP mice were stained for adiponectin, SM-alpha-actin, h-Caldesmon (blue, Cy5 secondary antibody), or fluorescent secondary antibodies only, and subjected to confocal microscopy analysis. Transgelin-lineage VSMC are green, non-SMC are red (tomato). Nuclei are stained with DAPI. No tomato red staining was detected.

    Techniques Used: Synthesized, Knock-Out, Mouse Assay, Confocal Microscopy, Staining, Marker, Isolation

    2) Product Images from "Neem leaf glycoprotein prevents post-surgical sarcoma recurrence in Swiss mice by differentially regulating cytotoxic T and myeloid-derived suppressor cells"

    Article Title: Neem leaf glycoprotein prevents post-surgical sarcoma recurrence in Swiss mice by differentially regulating cytotoxic T and myeloid-derived suppressor cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0175540

    CD8 + T cells downregulate MDSCs in Fas dependent pathway. (A) Percentage of Annexin V-PI + MDSCs within the blood of PBS, NLGP, CD8 + T cell depleted NLGP immunized mice (n = 6). (B) Flow cytometric assessment of Gr1 + FasR + MDSCs in post-surgery PBS-, NLGP-treated mice with or without CD8 + T cell depletion. (C) Expression of FasL within CD8 + T cells in mice with tumor surgery in PBS and NLGP immunized mice. (D) Flow cytometric assessment of Caspase 3 within Gr1 + MDSCs in PBS, NLGP and CD8 depleted NLGP immunized mice. (E) Protein level expression of Caspase 3, Caspase 8 and cFLIP within MDSCs from PBS, NLGP and CD8 depleted NLGP immunized surgically tumor removed mice. (n = 6, in each group). (F) Experimental design with MDSCs and CD8 + T cells. (G1) Expression of FasL within NLGP-treated CD8 + T cells. (G2) Expression of cFLIP and FasR within MDSCs in the presence and absence of supernatants from NLGP-treated CD8 + T cells, with or without IFNγ neutralization. (H) Assessment of the cytotoxic potential of NLGP-treated CD8 + T cells towards tumor-derived MDSCs, in the presence of Brefeldin A and Concanamycin A. (** p
    Figure Legend Snippet: CD8 + T cells downregulate MDSCs in Fas dependent pathway. (A) Percentage of Annexin V-PI + MDSCs within the blood of PBS, NLGP, CD8 + T cell depleted NLGP immunized mice (n = 6). (B) Flow cytometric assessment of Gr1 + FasR + MDSCs in post-surgery PBS-, NLGP-treated mice with or without CD8 + T cell depletion. (C) Expression of FasL within CD8 + T cells in mice with tumor surgery in PBS and NLGP immunized mice. (D) Flow cytometric assessment of Caspase 3 within Gr1 + MDSCs in PBS, NLGP and CD8 depleted NLGP immunized mice. (E) Protein level expression of Caspase 3, Caspase 8 and cFLIP within MDSCs from PBS, NLGP and CD8 depleted NLGP immunized surgically tumor removed mice. (n = 6, in each group). (F) Experimental design with MDSCs and CD8 + T cells. (G1) Expression of FasL within NLGP-treated CD8 + T cells. (G2) Expression of cFLIP and FasR within MDSCs in the presence and absence of supernatants from NLGP-treated CD8 + T cells, with or without IFNγ neutralization. (H) Assessment of the cytotoxic potential of NLGP-treated CD8 + T cells towards tumor-derived MDSCs, in the presence of Brefeldin A and Concanamycin A. (** p

    Techniques Used: Mouse Assay, Flow Cytometry, Expressing, Neutralization, Derivative Assay

    Related Articles

    Staining:

    Article Title: PGAP2 Is Essential for Correct Processing and Stable Expression of GPI-anchored Proteins D⃞
    Article Snippet: .. Cells were incubated with or without 5 μg/ml BFA (MP Biomedicals, Irvine, CA) for 10 min, and then fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) for 15 min. After quenching with 40 mM ammonium chloride in PBS, the cells were permeabilized with PBS containing 0.1% Triton-X (TX)-100 and 2.5% goat serum for 1 h at room temperature and stained with rabbit anti-PGAP2, mouse anti-GM130 (BD Biosciences) or mouse anti-myc (9E10) antibodies followed by an Alexa 488-conjugated donkey anti-mouse IgG antibody (Molecular Probes, Eugene, OR) or Alexa 594-conjugated goat anti-rabbit IgG antibody (Molecular Probes). .. The hybridoma producing the 9E10 antibody was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the National Institute of Child Health and Human Development and maintained by the Department of Biological Sciences, University of Iowa.

    Incubation:

    Article Title: PGAP2 Is Essential for Correct Processing and Stable Expression of GPI-anchored Proteins D⃞
    Article Snippet: .. Cells were incubated with or without 5 μg/ml BFA (MP Biomedicals, Irvine, CA) for 10 min, and then fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) for 15 min. After quenching with 40 mM ammonium chloride in PBS, the cells were permeabilized with PBS containing 0.1% Triton-X (TX)-100 and 2.5% goat serum for 1 h at room temperature and stained with rabbit anti-PGAP2, mouse anti-GM130 (BD Biosciences) or mouse anti-myc (9E10) antibodies followed by an Alexa 488-conjugated donkey anti-mouse IgG antibody (Molecular Probes, Eugene, OR) or Alexa 594-conjugated goat anti-rabbit IgG antibody (Molecular Probes). .. The hybridoma producing the 9E10 antibody was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the National Institute of Child Health and Human Development and maintained by the Department of Biological Sciences, University of Iowa.

    Article Title: 2D Kinetic Analysis of TCR and CD8 Coreceptor for LCMV GP33 Epitopes
    Article Snippet: .. Cells were incubated with peptide for 1 h at 37° Celsius in R10 media and washed before Brefeldin A was added (MP Biomedicals) and the cells incubated for another 4 h. R10 media was composed of RPMI 1640 (Mediatech), 10% heat inactivated FBS (Hyclone), 10 mM HEPES buffer (Mediatech), 2 mM L-glutamine (Mediatech), 50 μM 2-mercaptoethanol (2ME) (Sigma), and 100 μg/ml gentamicin (Mediatech). .. Additional samples were also cultured without peptide as a negative control and stimulation with a PMA (20 nM; Fisher Biotech) ionomycin (1 μM; Sigma) combination was used as a positive control.

    other:

    Article Title: Vascular smooth muscle cell-derived adiponectin: a paracrine regulator of contractile phenotype
    Article Snippet: Rosiglitazone was purchased from Enzo Life Sciences Inc. (Farmingdale, NY), Brefeldin A was from MP Biomedicals (Solon, OH).