diclofenac  (Valiant)

 
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    Name:
    Diclofenac
    Description:

    Catalog Number:
    0215766010
    Price:
    63.3
    Category:
    Life Sciences Biochemicals Enzyme Inhibitors
    Applications:
    Cyclooxygenase inhibitor
    Size:
    10 g
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    Structured Review

    Valiant diclofenac
    Diclofenac

    https://www.bioz.com/result/diclofenac/product/Valiant
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    diclofenac - by Bioz Stars, 2021-04
    90/100 stars

    Images

    1) Product Images from "Modulation of p73 isoforms expression induces anti-proliferative and pro-apoptotic activity in mantle cell lymphoma independent of p53 status"

    Article Title: Modulation of p73 isoforms expression induces anti-proliferative and pro-apoptotic activity in mantle cell lymphoma independent of p53 status

    Journal: Leukemia & lymphoma

    doi: 10.3109/10428194.2016.1165814

    Diclofenac treatment of MCL cells induces cell death. (A) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h. Then, cytospin preparations of the cells were stained with Hema-3 stain and representative images are shown. The solid arrows point to apoptotic cells and the dotted arrows indicate necroptosis. (B) and (C) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h. Cells were labeled with Annexin-V-Fluorescein and propidium iodide followed by (B) flow cytometric analysis or (C) fluorescence microscopy and representative images are shown (at 400×).
    Figure Legend Snippet: Diclofenac treatment of MCL cells induces cell death. (A) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h. Then, cytospin preparations of the cells were stained with Hema-3 stain and representative images are shown. The solid arrows point to apoptotic cells and the dotted arrows indicate necroptosis. (B) and (C) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h. Cells were labeled with Annexin-V-Fluorescein and propidium iodide followed by (B) flow cytometric analysis or (C) fluorescence microscopy and representative images are shown (at 400×).

    Techniques Used: Incubation, Staining, Labeling, Flow Cytometry, Fluorescence, Microscopy

    Diclofenac treatment of MCL cells induces TAp73 expression, p53 target genes and caspase cascade activation. (A and B) Diclofenac treatment of MCL cells enhances the expression of the p53 pro-apoptotic targets and differentially modulates the expression of p73 isoforms. MCL cell lines were incubated in media alone (control) or media with the indicated concentrations of diclofenac for the indicated times. Then, the mRNA expression of either: (A) TAp73, ΔNp73, DEC-1 or (B) PUMA , NOXA , or CD95 was analyzed by qRT-PCR and normalized to the housekeeping gene β-actin. The figure for DEC-1 expression in Granta was at 48 h of diclofenac treatment. (C) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 48 h. Then, Caspase Glo assay was performed and luminescence was measured after 30 min and plotted. (D) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h, Cytospin preparations of the cells were then immunostained with an antibody against cleaved caspase 3 (the active form of the effector caspase 3) and representative images are shown.
    Figure Legend Snippet: Diclofenac treatment of MCL cells induces TAp73 expression, p53 target genes and caspase cascade activation. (A and B) Diclofenac treatment of MCL cells enhances the expression of the p53 pro-apoptotic targets and differentially modulates the expression of p73 isoforms. MCL cell lines were incubated in media alone (control) or media with the indicated concentrations of diclofenac for the indicated times. Then, the mRNA expression of either: (A) TAp73, ΔNp73, DEC-1 or (B) PUMA , NOXA , or CD95 was analyzed by qRT-PCR and normalized to the housekeeping gene β-actin. The figure for DEC-1 expression in Granta was at 48 h of diclofenac treatment. (C) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 48 h. Then, Caspase Glo assay was performed and luminescence was measured after 30 min and plotted. (D) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h, Cytospin preparations of the cells were then immunostained with an antibody against cleaved caspase 3 (the active form of the effector caspase 3) and representative images are shown.

    Techniques Used: Expressing, Activation Assay, Incubation, Quantitative RT-PCR, Caspase-Glo Assay

    Diclofenac inhibits the growth on MCL cells. (A) MCL cell lines were incubated in media alone (control) or media with increasing concentrations (25, 50, 100, 150, 200, 250 μM) of diclofenac for 24 h. The MTT assay was performed and the percentage growth inhibition of the treated cells relative to their respective control was calculated with the equation [100 − (treated/control) − 100]. NS = not significant, *= p
    Figure Legend Snippet: Diclofenac inhibits the growth on MCL cells. (A) MCL cell lines were incubated in media alone (control) or media with increasing concentrations (25, 50, 100, 150, 200, 250 μM) of diclofenac for 24 h. The MTT assay was performed and the percentage growth inhibition of the treated cells relative to their respective control was calculated with the equation [100 − (treated/control) − 100]. NS = not significant, *= p

    Techniques Used: Incubation, MTT Assay, Inhibition

    Diclofenac treatment of MCL cells induces cell cycle arrest. (A) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 48 h. Cell cycle analysis was performed using propidium iodide followed by flow cytometric analysis. (B) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24, 48, or 72 h, and then mRNA expression of p21 was analyzed by qRT-PCR and normalized to the housekeeping gene β-actin. This is a representative of three experiments done in triplicate. (C) Cells were incubated in either media alone or media with diclofenac at different concentrations for 48 h, and then BrdU incorporation was measured. (D) Diclofenac treatment inhibits proliferation of MCL cells. MCL cell lines were incubated in media alone (control) or media with 50 μM of diclofenac for 48 h. Cytospin preparations of the cells were then immunostained with an antibody against the proliferation marker Ki-67 and representative images are shown. This is a representative of two experiments done in duplicate.
    Figure Legend Snippet: Diclofenac treatment of MCL cells induces cell cycle arrest. (A) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 48 h. Cell cycle analysis was performed using propidium iodide followed by flow cytometric analysis. (B) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24, 48, or 72 h, and then mRNA expression of p21 was analyzed by qRT-PCR and normalized to the housekeeping gene β-actin. This is a representative of three experiments done in triplicate. (C) Cells were incubated in either media alone or media with diclofenac at different concentrations for 48 h, and then BrdU incorporation was measured. (D) Diclofenac treatment inhibits proliferation of MCL cells. MCL cell lines were incubated in media alone (control) or media with 50 μM of diclofenac for 48 h. Cytospin preparations of the cells were then immunostained with an antibody against the proliferation marker Ki-67 and representative images are shown. This is a representative of two experiments done in duplicate.

    Techniques Used: Incubation, Cell Cycle Assay, Flow Cytometry, Expressing, Quantitative RT-PCR, BrdU Incorporation Assay, Marker

    Diclofenac inhibits the growth of resistant MCL cells and primary MCL cells. (A) and (D) The resistant MCL cell lines (GRK, GRL, GRR) or patient-derived primary MCL cells (MCL-P1) were incubated in media alone (control) or media with increasing doses (25, 50, 100, 150, 200, 250 μM) of diclofenac for the indicated durations. The MTT assay was done and SigmaPlot 11 (Systat Software, CA) was used to plot the standard curves and calculate the EC 50 . (B) and (E) GRK, GRL, GRR, or MCL-P1 were incubated in media alone (control) or media with increasing doses (25, 50, 100, 150, 200, 250 μM) of diclofenac for 24 h, and the percentage growth inhibition of the treated cells relative to their respective control was calculated with the equation [100 − (treated/control) × 100]. (C) and (F) MCL cells were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h. Cytospin preparations of the cells were then stained with Hema-3 stain and representative images are shown. This is representative of three experiments done in triplicate.
    Figure Legend Snippet: Diclofenac inhibits the growth of resistant MCL cells and primary MCL cells. (A) and (D) The resistant MCL cell lines (GRK, GRL, GRR) or patient-derived primary MCL cells (MCL-P1) were incubated in media alone (control) or media with increasing doses (25, 50, 100, 150, 200, 250 μM) of diclofenac for the indicated durations. The MTT assay was done and SigmaPlot 11 (Systat Software, CA) was used to plot the standard curves and calculate the EC 50 . (B) and (E) GRK, GRL, GRR, or MCL-P1 were incubated in media alone (control) or media with increasing doses (25, 50, 100, 150, 200, 250 μM) of diclofenac for 24 h, and the percentage growth inhibition of the treated cells relative to their respective control was calculated with the equation [100 − (treated/control) × 100]. (C) and (F) MCL cells were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h. Cytospin preparations of the cells were then stained with Hema-3 stain and representative images are shown. This is representative of three experiments done in triplicate.

    Techniques Used: Derivative Assay, Incubation, MTT Assay, Software, Inhibition, Staining

    p73 modulation alters the behavior of MCL cells. (A) Granta-519 cells were transfected with a TAp73 mammalian expression vector or control vector, and the over-expression of TAp73 was confirmed using RT-PCR for TAp73 mRNA and immunocyto-chemistry for TAp73 protein (200×). The MTT assay was used to compare the basal growth of the cells with serum deprivation (2.5 or 0% serum) or with doxorubicin (0, 0.5, 1, 1.5, 2 μM) for 48 h. (B) Jeko-1 cells were transfected with p73 siRNA or control siRNA and the knockdown was confirmed by qRT-PCR for TAp73. After 48 h post-transfection, cells were treated with the indicated concentrations of diclofenac and the MTT assay was used to compare the growth of the cells.
    Figure Legend Snippet: p73 modulation alters the behavior of MCL cells. (A) Granta-519 cells were transfected with a TAp73 mammalian expression vector or control vector, and the over-expression of TAp73 was confirmed using RT-PCR for TAp73 mRNA and immunocyto-chemistry for TAp73 protein (200×). The MTT assay was used to compare the basal growth of the cells with serum deprivation (2.5 or 0% serum) or with doxorubicin (0, 0.5, 1, 1.5, 2 μM) for 48 h. (B) Jeko-1 cells were transfected with p73 siRNA or control siRNA and the knockdown was confirmed by qRT-PCR for TAp73. After 48 h post-transfection, cells were treated with the indicated concentrations of diclofenac and the MTT assay was used to compare the growth of the cells.

    Techniques Used: Transfection, Expressing, Plasmid Preparation, Over Expression, Reverse Transcription Polymerase Chain Reaction, MTT Assay, Quantitative RT-PCR

    2) Product Images from "Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography"

    Article Title: Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography

    Journal: BMC Research Notes

    doi: 10.1186/s13104-016-2358-y

    Elution of flavone B from colon. HPLC chromatographs of a blank colon; b colon spiked with internal standard (diclofenac 25 µg/mL); c colon spiked with flavone B (100 µg/mL) and internal standard
    Figure Legend Snippet: Elution of flavone B from colon. HPLC chromatographs of a blank colon; b colon spiked with internal standard (diclofenac 25 µg/mL); c colon spiked with flavone B (100 µg/mL) and internal standard

    Techniques Used: High Performance Liquid Chromatography

    3) Product Images from "Design and Optimization of PLGA-Based Diclofenac Loaded Nanoparticles"

    Article Title: Design and Optimization of PLGA-Based Diclofenac Loaded Nanoparticles

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0087326

    In vitro drug release study with 0.25% stabilizer concentrations. In vitro release profile of diclofenac sodium in phosphate buffer of pH 7 from 0.25% PVA formulated NPs and 0.25% DMAB formulated NPs (mean ± SD, n = 3, p
    Figure Legend Snippet: In vitro drug release study with 0.25% stabilizer concentrations. In vitro release profile of diclofenac sodium in phosphate buffer of pH 7 from 0.25% PVA formulated NPs and 0.25% DMAB formulated NPs (mean ± SD, n = 3, p

    Techniques Used: In Vitro

    Entrapment effects of varying DMAB stabilizer concentrations. Entrapment efficiency after 8,800(A) and entrapment efficiency after 12,000 rpm centrifugation of diclofenac loaded NPs (B). Values are expressed as mean ± standard deviation.
    Figure Legend Snippet: Entrapment effects of varying DMAB stabilizer concentrations. Entrapment efficiency after 8,800(A) and entrapment efficiency after 12,000 rpm centrifugation of diclofenac loaded NPs (B). Values are expressed as mean ± standard deviation.

    Techniques Used: Centrifugation, Standard Deviation

    Morphological analysis of 0.25% DMAB formulated NPs. Transmission electron microscopy image of empty NPs formulated with 0.25% DMAB stabilizer (A) and transmission electron microscopy image of diclofenac loaded NPs formulated with 0.25% DMAB stabilizer (B).
    Figure Legend Snippet: Morphological analysis of 0.25% DMAB formulated NPs. Transmission electron microscopy image of empty NPs formulated with 0.25% DMAB stabilizer (A) and transmission electron microscopy image of diclofenac loaded NPs formulated with 0.25% DMAB stabilizer (B).

    Techniques Used: Transmission Assay, Electron Microscopy

    Chemical structure of diclofenac sodium [ 46 ] .
    Figure Legend Snippet: Chemical structure of diclofenac sodium [ 46 ] .

    Techniques Used:

    Particle Sizing Systems NICOMP analysis of diclofenac loaded 0.1% DMAB NP formulation.
    Figure Legend Snippet: Particle Sizing Systems NICOMP analysis of diclofenac loaded 0.1% DMAB NP formulation.

    Techniques Used:

    Morphological analysis of 1% PVA formulated NPs. Transmission electron microscopy image of empty NPs formulated with 1% PVA stabilizer (A) and transmission electron microscopy image of diclofenac loaded NPs formulated with 1% PVA stabilizer (B).
    Figure Legend Snippet: Morphological analysis of 1% PVA formulated NPs. Transmission electron microscopy image of empty NPs formulated with 1% PVA stabilizer (A) and transmission electron microscopy image of diclofenac loaded NPs formulated with 1% PVA stabilizer (B).

    Techniques Used: Transmission Assay, Electron Microscopy

    In vitro drug release study with 0.1% stabilizer concentrations. In vitro release profile of diclofenac sodium in phosphate buffer of pH 7 from 0.1% PVA formulated NPs and 0.1% DMAB formulated NPs (mean ± SD, n = 3, p
    Figure Legend Snippet: In vitro drug release study with 0.1% stabilizer concentrations. In vitro release profile of diclofenac sodium in phosphate buffer of pH 7 from 0.1% PVA formulated NPs and 0.1% DMAB formulated NPs (mean ± SD, n = 3, p

    Techniques Used: In Vitro

    Related Articles

    other:

    Article Title: A Sensitive and Specific CYP Cocktail Assay for the Simultaneous Assessment of Human Cytochrome P450 Activities in Primary Cultures of Human Hepatocytes using LC-MS/MS
    Article Snippet: Diclofenac sodium salt and dextrorphan-D-Tartrate were purchased from MP Biomedical Inc. (Solon, OH, USA).

    Article Title: Development of an ultra fast online-solid phase extraction (SPE) liquid chromatography electrospray tandem mass spectrometry (LC-ESI-MS/MS) based approach for the determination of drugs in pharmacokinetic studies †
    Article Snippet: Diclofenac sodium salt ( > 98% purity) was purchased from MP Biomedicals LLC (Solon, Ohio).

    Injection:

    Article Title: Protective effects of calcium gluconate on osteoarthritis induced by anterior cruciate ligament transection and partial medial meniscectomy in Sprague-Dawley rats
    Article Snippet: BrdU uptake Proliferating cells were labeled by means of an intraperitoneal injection of 5-bromo-2′-deoxyuridine (BrdU). .. One hour prior to injection of diclofenac sodium or oral administration of calcium gluconate (day 82 of treatment), rats received intraperitoneal injections of BrdU (MP Biomedicals, Solon, OH, USA; 50 mg/kg), in a volume of 2 ml/kg, dissolved in saline; the animals were sacrificed 72 h later. .. BrdU uptake was evaluated by immunohistochemistry using an anti-BrdU antibody.

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    Valiant diclofenac
    <t>Diclofenac</t> treatment of MCL cells induces cell death. (A) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h. Then, cytospin preparations of the cells were stained with Hema-3 stain and representative images are shown. The solid arrows point to apoptotic cells and the dotted arrows indicate necroptosis. (B) and (C) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h. Cells were labeled with Annexin-V-Fluorescein and propidium iodide followed by (B) flow cytometric analysis or (C) fluorescence microscopy and representative images are shown (at 400×).
    Diclofenac, supplied by Valiant, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/diclofenac/product/Valiant
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    diclofenac - by Bioz Stars, 2021-04
    90/100 stars
      Buy from Supplier

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    Diclofenac treatment of MCL cells induces cell death. (A) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h. Then, cytospin preparations of the cells were stained with Hema-3 stain and representative images are shown. The solid arrows point to apoptotic cells and the dotted arrows indicate necroptosis. (B) and (C) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h. Cells were labeled with Annexin-V-Fluorescein and propidium iodide followed by (B) flow cytometric analysis or (C) fluorescence microscopy and representative images are shown (at 400×).

    Journal: Leukemia & lymphoma

    Article Title: Modulation of p73 isoforms expression induces anti-proliferative and pro-apoptotic activity in mantle cell lymphoma independent of p53 status

    doi: 10.3109/10428194.2016.1165814

    Figure Lengend Snippet: Diclofenac treatment of MCL cells induces cell death. (A) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h. Then, cytospin preparations of the cells were stained with Hema-3 stain and representative images are shown. The solid arrows point to apoptotic cells and the dotted arrows indicate necroptosis. (B) and (C) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h. Cells were labeled with Annexin-V-Fluorescein and propidium iodide followed by (B) flow cytometric analysis or (C) fluorescence microscopy and representative images are shown (at 400×).

    Article Snippet: Diclofenac was purchased from MP Biomedicals (Solon, OH).

    Techniques: Incubation, Staining, Labeling, Flow Cytometry, Fluorescence, Microscopy

    Diclofenac treatment of MCL cells induces TAp73 expression, p53 target genes and caspase cascade activation. (A and B) Diclofenac treatment of MCL cells enhances the expression of the p53 pro-apoptotic targets and differentially modulates the expression of p73 isoforms. MCL cell lines were incubated in media alone (control) or media with the indicated concentrations of diclofenac for the indicated times. Then, the mRNA expression of either: (A) TAp73, ΔNp73, DEC-1 or (B) PUMA , NOXA , or CD95 was analyzed by qRT-PCR and normalized to the housekeeping gene β-actin. The figure for DEC-1 expression in Granta was at 48 h of diclofenac treatment. (C) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 48 h. Then, Caspase Glo assay was performed and luminescence was measured after 30 min and plotted. (D) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h, Cytospin preparations of the cells were then immunostained with an antibody against cleaved caspase 3 (the active form of the effector caspase 3) and representative images are shown.

    Journal: Leukemia & lymphoma

    Article Title: Modulation of p73 isoforms expression induces anti-proliferative and pro-apoptotic activity in mantle cell lymphoma independent of p53 status

    doi: 10.3109/10428194.2016.1165814

    Figure Lengend Snippet: Diclofenac treatment of MCL cells induces TAp73 expression, p53 target genes and caspase cascade activation. (A and B) Diclofenac treatment of MCL cells enhances the expression of the p53 pro-apoptotic targets and differentially modulates the expression of p73 isoforms. MCL cell lines were incubated in media alone (control) or media with the indicated concentrations of diclofenac for the indicated times. Then, the mRNA expression of either: (A) TAp73, ΔNp73, DEC-1 or (B) PUMA , NOXA , or CD95 was analyzed by qRT-PCR and normalized to the housekeeping gene β-actin. The figure for DEC-1 expression in Granta was at 48 h of diclofenac treatment. (C) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 48 h. Then, Caspase Glo assay was performed and luminescence was measured after 30 min and plotted. (D) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h, Cytospin preparations of the cells were then immunostained with an antibody against cleaved caspase 3 (the active form of the effector caspase 3) and representative images are shown.

    Article Snippet: Diclofenac was purchased from MP Biomedicals (Solon, OH).

    Techniques: Expressing, Activation Assay, Incubation, Quantitative RT-PCR, Caspase-Glo Assay

    Diclofenac inhibits the growth on MCL cells. (A) MCL cell lines were incubated in media alone (control) or media with increasing concentrations (25, 50, 100, 150, 200, 250 μM) of diclofenac for 24 h. The MTT assay was performed and the percentage growth inhibition of the treated cells relative to their respective control was calculated with the equation [100 − (treated/control) − 100]. NS = not significant, *= p

    Journal: Leukemia & lymphoma

    Article Title: Modulation of p73 isoforms expression induces anti-proliferative and pro-apoptotic activity in mantle cell lymphoma independent of p53 status

    doi: 10.3109/10428194.2016.1165814

    Figure Lengend Snippet: Diclofenac inhibits the growth on MCL cells. (A) MCL cell lines were incubated in media alone (control) or media with increasing concentrations (25, 50, 100, 150, 200, 250 μM) of diclofenac for 24 h. The MTT assay was performed and the percentage growth inhibition of the treated cells relative to their respective control was calculated with the equation [100 − (treated/control) − 100]. NS = not significant, *= p

    Article Snippet: Diclofenac was purchased from MP Biomedicals (Solon, OH).

    Techniques: Incubation, MTT Assay, Inhibition

    Diclofenac treatment of MCL cells induces cell cycle arrest. (A) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 48 h. Cell cycle analysis was performed using propidium iodide followed by flow cytometric analysis. (B) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24, 48, or 72 h, and then mRNA expression of p21 was analyzed by qRT-PCR and normalized to the housekeeping gene β-actin. This is a representative of three experiments done in triplicate. (C) Cells were incubated in either media alone or media with diclofenac at different concentrations for 48 h, and then BrdU incorporation was measured. (D) Diclofenac treatment inhibits proliferation of MCL cells. MCL cell lines were incubated in media alone (control) or media with 50 μM of diclofenac for 48 h. Cytospin preparations of the cells were then immunostained with an antibody against the proliferation marker Ki-67 and representative images are shown. This is a representative of two experiments done in duplicate.

    Journal: Leukemia & lymphoma

    Article Title: Modulation of p73 isoforms expression induces anti-proliferative and pro-apoptotic activity in mantle cell lymphoma independent of p53 status

    doi: 10.3109/10428194.2016.1165814

    Figure Lengend Snippet: Diclofenac treatment of MCL cells induces cell cycle arrest. (A) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 48 h. Cell cycle analysis was performed using propidium iodide followed by flow cytometric analysis. (B) MCL cell lines were incubated in media alone (control) or media with 100 μM of diclofenac for 24, 48, or 72 h, and then mRNA expression of p21 was analyzed by qRT-PCR and normalized to the housekeeping gene β-actin. This is a representative of three experiments done in triplicate. (C) Cells were incubated in either media alone or media with diclofenac at different concentrations for 48 h, and then BrdU incorporation was measured. (D) Diclofenac treatment inhibits proliferation of MCL cells. MCL cell lines were incubated in media alone (control) or media with 50 μM of diclofenac for 48 h. Cytospin preparations of the cells were then immunostained with an antibody against the proliferation marker Ki-67 and representative images are shown. This is a representative of two experiments done in duplicate.

    Article Snippet: Diclofenac was purchased from MP Biomedicals (Solon, OH).

    Techniques: Incubation, Cell Cycle Assay, Flow Cytometry, Expressing, Quantitative RT-PCR, BrdU Incorporation Assay, Marker

    Diclofenac inhibits the growth of resistant MCL cells and primary MCL cells. (A) and (D) The resistant MCL cell lines (GRK, GRL, GRR) or patient-derived primary MCL cells (MCL-P1) were incubated in media alone (control) or media with increasing doses (25, 50, 100, 150, 200, 250 μM) of diclofenac for the indicated durations. The MTT assay was done and SigmaPlot 11 (Systat Software, CA) was used to plot the standard curves and calculate the EC 50 . (B) and (E) GRK, GRL, GRR, or MCL-P1 were incubated in media alone (control) or media with increasing doses (25, 50, 100, 150, 200, 250 μM) of diclofenac for 24 h, and the percentage growth inhibition of the treated cells relative to their respective control was calculated with the equation [100 − (treated/control) × 100]. (C) and (F) MCL cells were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h. Cytospin preparations of the cells were then stained with Hema-3 stain and representative images are shown. This is representative of three experiments done in triplicate.

    Journal: Leukemia & lymphoma

    Article Title: Modulation of p73 isoforms expression induces anti-proliferative and pro-apoptotic activity in mantle cell lymphoma independent of p53 status

    doi: 10.3109/10428194.2016.1165814

    Figure Lengend Snippet: Diclofenac inhibits the growth of resistant MCL cells and primary MCL cells. (A) and (D) The resistant MCL cell lines (GRK, GRL, GRR) or patient-derived primary MCL cells (MCL-P1) were incubated in media alone (control) or media with increasing doses (25, 50, 100, 150, 200, 250 μM) of diclofenac for the indicated durations. The MTT assay was done and SigmaPlot 11 (Systat Software, CA) was used to plot the standard curves and calculate the EC 50 . (B) and (E) GRK, GRL, GRR, or MCL-P1 were incubated in media alone (control) or media with increasing doses (25, 50, 100, 150, 200, 250 μM) of diclofenac for 24 h, and the percentage growth inhibition of the treated cells relative to their respective control was calculated with the equation [100 − (treated/control) × 100]. (C) and (F) MCL cells were incubated in media alone (control) or media with 100 μM of diclofenac for 24 or 48 h. Cytospin preparations of the cells were then stained with Hema-3 stain and representative images are shown. This is representative of three experiments done in triplicate.

    Article Snippet: Diclofenac was purchased from MP Biomedicals (Solon, OH).

    Techniques: Derivative Assay, Incubation, MTT Assay, Software, Inhibition, Staining

    p73 modulation alters the behavior of MCL cells. (A) Granta-519 cells were transfected with a TAp73 mammalian expression vector or control vector, and the over-expression of TAp73 was confirmed using RT-PCR for TAp73 mRNA and immunocyto-chemistry for TAp73 protein (200×). The MTT assay was used to compare the basal growth of the cells with serum deprivation (2.5 or 0% serum) or with doxorubicin (0, 0.5, 1, 1.5, 2 μM) for 48 h. (B) Jeko-1 cells were transfected with p73 siRNA or control siRNA and the knockdown was confirmed by qRT-PCR for TAp73. After 48 h post-transfection, cells were treated with the indicated concentrations of diclofenac and the MTT assay was used to compare the growth of the cells.

    Journal: Leukemia & lymphoma

    Article Title: Modulation of p73 isoforms expression induces anti-proliferative and pro-apoptotic activity in mantle cell lymphoma independent of p53 status

    doi: 10.3109/10428194.2016.1165814

    Figure Lengend Snippet: p73 modulation alters the behavior of MCL cells. (A) Granta-519 cells were transfected with a TAp73 mammalian expression vector or control vector, and the over-expression of TAp73 was confirmed using RT-PCR for TAp73 mRNA and immunocyto-chemistry for TAp73 protein (200×). The MTT assay was used to compare the basal growth of the cells with serum deprivation (2.5 or 0% serum) or with doxorubicin (0, 0.5, 1, 1.5, 2 μM) for 48 h. (B) Jeko-1 cells were transfected with p73 siRNA or control siRNA and the knockdown was confirmed by qRT-PCR for TAp73. After 48 h post-transfection, cells were treated with the indicated concentrations of diclofenac and the MTT assay was used to compare the growth of the cells.

    Article Snippet: Diclofenac was purchased from MP Biomedicals (Solon, OH).

    Techniques: Transfection, Expressing, Plasmid Preparation, Over Expression, Reverse Transcription Polymerase Chain Reaction, MTT Assay, Quantitative RT-PCR

    Principal component analysis scores (PC1, PC2, and PC3) for protein pattern assessment of control and treated eleutheroembryos with (a) Rotenone, (b) DNOC, and (c) Diclofenac. Control groups are indicated with c. Three independent replicates were performed for all treatment groups (EC 10 treatment with either Rotenone, DNOC, or Diclofenac).

    Journal: International Journal of Proteomics

    Article Title: Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

    doi: 10.1155/2010/630134

    Figure Lengend Snippet: Principal component analysis scores (PC1, PC2, and PC3) for protein pattern assessment of control and treated eleutheroembryos with (a) Rotenone, (b) DNOC, and (c) Diclofenac. Control groups are indicated with c. Three independent replicates were performed for all treatment groups (EC 10 treatment with either Rotenone, DNOC, or Diclofenac).

    Article Snippet: All substances were of analytical grade and purchased from Riedel de Haen (Rotenone and DNOC) and MP Biomedicals (Diclofenac), respectively.

    Techniques:

    Summary of results from spot-to-spot analysis of proteomics experiments with Diclofenac (Dic), Rotenone(R), and DNOC (D). For all tested concentrations the percentages of protein spots, related to all detected protein spots in the 2-DE experiment, are depicted that show significant ( P

    Journal: International Journal of Proteomics

    Article Title: Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

    doi: 10.1155/2010/630134

    Figure Lengend Snippet: Summary of results from spot-to-spot analysis of proteomics experiments with Diclofenac (Dic), Rotenone(R), and DNOC (D). For all tested concentrations the percentages of protein spots, related to all detected protein spots in the 2-DE experiment, are depicted that show significant ( P

    Article Snippet: All substances were of analytical grade and purchased from Riedel de Haen (Rotenone and DNOC) and MP Biomedicals (Diclofenac), respectively.

    Techniques:

    Microscopically visible lethal effects of Rotenone, DNOC and Diclofenac to eleuthero-embryos studied after 48 h exposure. Concentration-effect relationships based on a logistic model are shown. Parameter estimates were as follows: Rotenone: EC 50 [ μ M] = 0.068 ± 0.00, p = 8.750 ± 2.25, DNOC: EC 50 [ μ M] = 3.007 ± 0.05, p = 12.311 ± 1.80, Diclofenac: EC 50 [ μ M] = 23.076 ± 1.08, p = 4.746 ± 1.65.

    Journal: International Journal of Proteomics

    Article Title: Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

    doi: 10.1155/2010/630134

    Figure Lengend Snippet: Microscopically visible lethal effects of Rotenone, DNOC and Diclofenac to eleuthero-embryos studied after 48 h exposure. Concentration-effect relationships based on a logistic model are shown. Parameter estimates were as follows: Rotenone: EC 50 [ μ M] = 0.068 ± 0.00, p = 8.750 ± 2.25, DNOC: EC 50 [ μ M] = 3.007 ± 0.05, p = 12.311 ± 1.80, Diclofenac: EC 50 [ μ M] = 23.076 ± 1.08, p = 4.746 ± 1.65.

    Article Snippet: All substances were of analytical grade and purchased from Riedel de Haen (Rotenone and DNOC) and MP Biomedicals (Diclofenac), respectively.

    Techniques: Concentration Assay

    2-DE gels from proteomics experiments: (a) control conditions, (b) EC 10 treatment with Rotenone, (c), EC 10 treatment with DNOC and (d) EC 10 treatment with Diclofenac. All protein samples were separated under the same 2-DE conditions: Immobiline strips pH 4–7 in the first and 14% PAA gels in the second dimension.

    Journal: International Journal of Proteomics

    Article Title: Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

    doi: 10.1155/2010/630134

    Figure Lengend Snippet: 2-DE gels from proteomics experiments: (a) control conditions, (b) EC 10 treatment with Rotenone, (c), EC 10 treatment with DNOC and (d) EC 10 treatment with Diclofenac. All protein samples were separated under the same 2-DE conditions: Immobiline strips pH 4–7 in the first and 14% PAA gels in the second dimension.

    Article Snippet: All substances were of analytical grade and purchased from Riedel de Haen (Rotenone and DNOC) and MP Biomedicals (Diclofenac), respectively.

    Techniques:

    Comparison of results from toxicoproteomics experiments with Rotenone, DNOC, and Diclofenac exposure. (a) PCA scores when all samples are included in PCA analysis. (b) Venn-Diagramm based on results from substance-specific univariate spot-to-spot analysis. Most of the protein spots changed specifically. 9 proteins concurrently changed after Rotenone, DNOC, and Diclofenac treatment. Moreover, 13 proteins simultaneously changed after Rotenone and DNOC treatment, and 14 proteins showed changed expression levels at exposure against Diclofenac or Rotenone. Only one protein was found to be changed in the larval protein samples after DNOC as well as Diclofenac treatment. (c) 2-DE gel from eleutheroembryonal protein samples. All proteins that significantly changed in expression in at least two toxicoproteomics experiments (treatment with either Rotenone, DNOC, or Diclofenac) are labelled: Protein labels of protein spots simultaneously altered in Rotenone, DNOC, and Diclofenac experiments are framed (□), in Rotenone and DNOC experiments are underlined (—), in Rotenone and Diclofenac experiments are dashed (—), and in DNOC, and Diclofenac experiments are labelled by name only.

    Journal: International Journal of Proteomics

    Article Title: Proteomic Signatures of the Zebrafish (Danio rerio) Embryo: Sensitivity and Specificity in Toxicity Assessment of Chemicals

    doi: 10.1155/2010/630134

    Figure Lengend Snippet: Comparison of results from toxicoproteomics experiments with Rotenone, DNOC, and Diclofenac exposure. (a) PCA scores when all samples are included in PCA analysis. (b) Venn-Diagramm based on results from substance-specific univariate spot-to-spot analysis. Most of the protein spots changed specifically. 9 proteins concurrently changed after Rotenone, DNOC, and Diclofenac treatment. Moreover, 13 proteins simultaneously changed after Rotenone and DNOC treatment, and 14 proteins showed changed expression levels at exposure against Diclofenac or Rotenone. Only one protein was found to be changed in the larval protein samples after DNOC as well as Diclofenac treatment. (c) 2-DE gel from eleutheroembryonal protein samples. All proteins that significantly changed in expression in at least two toxicoproteomics experiments (treatment with either Rotenone, DNOC, or Diclofenac) are labelled: Protein labels of protein spots simultaneously altered in Rotenone, DNOC, and Diclofenac experiments are framed (□), in Rotenone and DNOC experiments are underlined (—), in Rotenone and Diclofenac experiments are dashed (—), and in DNOC, and Diclofenac experiments are labelled by name only.

    Article Snippet: All substances were of analytical grade and purchased from Riedel de Haen (Rotenone and DNOC) and MP Biomedicals (Diclofenac), respectively.

    Techniques: Expressing

    Elution of flavone B from colon. HPLC chromatographs of a blank colon; b colon spiked with internal standard (diclofenac 25 µg/mL); c colon spiked with flavone B (100 µg/mL) and internal standard

    Journal: BMC Research Notes

    Article Title: Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography

    doi: 10.1186/s13104-016-2358-y

    Figure Lengend Snippet: Elution of flavone B from colon. HPLC chromatographs of a blank colon; b colon spiked with internal standard (diclofenac 25 µg/mL); c colon spiked with flavone B (100 µg/mL) and internal standard

    Article Snippet: Stock solutions of 100 µg/mL of flavone B, prepared as described previously [ ], and 25 µg/mL diclofenac (MP Biomedicals, LLC; Solon, OH) were prepared with acetonitrile/water/acetic acid/trimethylamine (70:30:0.2:0.05).

    Techniques: High Performance Liquid Chromatography

    Histopathological observations of the tibial and femoral articular cartilage. Of sham controls (A, B) , OA controls (C, D) , and the diclofenac sodium (E, F) or calcium gluconate 100 mg/kg (G, H) , 50 mg/kg (I, J), and 25 mg/kg (K, L) -treated groups. Histopathological changes were markedly inhibited by calcium gluconate. Arrows indicate the thickness of tibia or femur articular cartilage; OA, osteoarthritis; gaps indicate the intra-joint space between the articular surface of the tibia and femur. Scale bar = 80 μm.

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: Protective effects of calcium gluconate on osteoarthritis induced by anterior cruciate ligament transection and partial medial meniscectomy in Sprague-Dawley rats

    doi: 10.1186/1749-799X-9-14

    Figure Lengend Snippet: Histopathological observations of the tibial and femoral articular cartilage. Of sham controls (A, B) , OA controls (C, D) , and the diclofenac sodium (E, F) or calcium gluconate 100 mg/kg (G, H) , 50 mg/kg (I, J), and 25 mg/kg (K, L) -treated groups. Histopathological changes were markedly inhibited by calcium gluconate. Arrows indicate the thickness of tibia or femur articular cartilage; OA, osteoarthritis; gaps indicate the intra-joint space between the articular surface of the tibia and femur. Scale bar = 80 μm.

    Article Snippet: One hour prior to injection of diclofenac sodium or oral administration of calcium gluconate (day 82 of treatment), rats received intraperitoneal injections of BrdU (MP Biomedicals, Solon, OH, USA; 50 mg/kg), in a volume of 2 ml/kg, dissolved in saline; the animals were sacrificed 72 h later.

    Techniques:

    BrdU, caspase-3, PARP, and COX-2-immunoreative cells in the tibial and femoral articular cartilage. Of sham controls (A, B) , OA controls (C, D) , and the diclofenac sodium (E, F) or calcium gluconate 100 mg/kg (G, H) , 50 mg/kg (I, J) and 25 mg/kg (K, L) -treated groups. Changes in the numbers of immunoreactive cells were significantly ( p

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: Protective effects of calcium gluconate on osteoarthritis induced by anterior cruciate ligament transection and partial medial meniscectomy in Sprague-Dawley rats

    doi: 10.1186/1749-799X-9-14

    Figure Lengend Snippet: BrdU, caspase-3, PARP, and COX-2-immunoreative cells in the tibial and femoral articular cartilage. Of sham controls (A, B) , OA controls (C, D) , and the diclofenac sodium (E, F) or calcium gluconate 100 mg/kg (G, H) , 50 mg/kg (I, J) and 25 mg/kg (K, L) -treated groups. Changes in the numbers of immunoreactive cells were significantly ( p

    Article Snippet: One hour prior to injection of diclofenac sodium or oral administration of calcium gluconate (day 82 of treatment), rats received intraperitoneal injections of BrdU (MP Biomedicals, Solon, OH, USA; 50 mg/kg), in a volume of 2 ml/kg, dissolved in saline; the animals were sacrificed 72 h later.

    Techniques: