egf  (Valiant)

 
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    Name:
    Epidermal growth factor human recombinant 98
    Description:
    Epidermal Growth Factor Human Recombinant
    Catalog Number:
    0215457180
    Price:
    228.55
    Category:
    Life Sciences Biochemicals Proteins and Derivatives Natural Recombinant Proteins
    Applications:
    Cell culture
    Size:
    100 μg
    Buy from Supplier


    Structured Review

    Valiant egf
    Epidermal Growth Factor Human Recombinant
    https://www.bioz.com/result/egf/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egf - by Bioz Stars, 2021-04
    93/100 stars

    Images

    Related Articles

    In Vitro:

    Article Title: A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos
    Article Snippet: .. Culture media Oocyte in vitro maturation (IVM) was carried out in Medium 199 (Invitrogen, Gibco 11150-059, Grand Island, NY, USA) supplemented with 0.1 mg/ml sodium pyruvate, 76 µg/ml penicillin-G, 50 µg/ml streptomycin sulphate, 2.0 mM Glutamax (Gibco), 100 mM cysteamine, 10 ng/ml epidermal growth factor (EGF), 10 IU/ml equine chorionic gonadotropin (eCG), 10 IU/ml human chorionic gonadotropin (hCG), 0.1% fatty acid-free bovine serum albumin (BSA; IVP grade gamma irradiated, MP Biomedicals, Auckland, New Zealand), and 10% porcine follicular fluid [ ]. ..

    Article Title: PRMT1-mediated methylation of the EGF receptor regulates signaling and cetuximab response
    Article Snippet: GST-tagged recombinant proteins were then purified using glutathione sepharose 4B (GE Healthcare) and dialyzed against phosphate-buffered saline. .. To perform in vitro methylation assay, GST-tagged recombinant PRMT1 and EGFR fragments were incubated together in the presence of 2.2 Ci S-adenosyl-L-[methyl-3 H] methionine (85 Ci/mmol from a 0.55 mCi/ml stock solution; MP Biomedicals) for 1 hour at 30°C in a final volume of 50 μl of phosphate-buffered saline. .. After incubation, samples were separated by SDS-PAGE and methylation levels were examined by fluorography.

    Irradiation:

    Article Title: A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos
    Article Snippet: .. Culture media Oocyte in vitro maturation (IVM) was carried out in Medium 199 (Invitrogen, Gibco 11150-059, Grand Island, NY, USA) supplemented with 0.1 mg/ml sodium pyruvate, 76 µg/ml penicillin-G, 50 µg/ml streptomycin sulphate, 2.0 mM Glutamax (Gibco), 100 mM cysteamine, 10 ng/ml epidermal growth factor (EGF), 10 IU/ml equine chorionic gonadotropin (eCG), 10 IU/ml human chorionic gonadotropin (hCG), 0.1% fatty acid-free bovine serum albumin (BSA; IVP grade gamma irradiated, MP Biomedicals, Auckland, New Zealand), and 10% porcine follicular fluid [ ]. ..

    Incubation:

    Article Title: Differential Effects of EGFR Ligands on Endocytic Sorting of the Receptor
    Article Snippet: EGFR content was then measured using an EGFR ELISA kit (RnD Systems). .. EGFR degradation analysis using 35 S methionine/cysteine labelling Serum-starved cells were pre-incubated in methionine- /cysteine-free DMEM supplemented with 1 mm sodium pyruvate, 2 mm glutamax, 200 U/mL penicillin, and 50 ng/mL streptomycin for 1 h. After preincubation, cells were labelled with 0.25 mCi/mL L- [35 S]-methionine/cysteine (Mpbiomedicals) for 1–2 h. Cells were changed to starvation medium and allowed to synthesize protein for 3 h. After 3 h cells were incubated with ligands for 1 h on ice, then washed in starvation medium, and incubated in starvation medium at 37°C for 0–6 h. At the indicated times, cells were rinsed in cold PBS and then scraped off in RIPA lysis buffer supplemented with Protease Inhibitor Cocktail Set II and Phosphatase Inhibitor Cocktail Set III. .. Cell debris was removed by spinning at 13,000 ×g ; the lysates were pre-cleared with protein G Dynabeads (Invitrogen), and EGFR immunoprecipitated with mouse monoclonal anti-EGFR antibody (clone 199.12) and protein G Dynabeads.

    Article Title: PRMT1-mediated methylation of the EGF receptor regulates signaling and cetuximab response
    Article Snippet: GST-tagged recombinant proteins were then purified using glutathione sepharose 4B (GE Healthcare) and dialyzed against phosphate-buffered saline. .. To perform in vitro methylation assay, GST-tagged recombinant PRMT1 and EGFR fragments were incubated together in the presence of 2.2 Ci S-adenosyl-L-[methyl-3 H] methionine (85 Ci/mmol from a 0.55 mCi/ml stock solution; MP Biomedicals) for 1 hour at 30°C in a final volume of 50 μl of phosphate-buffered saline. .. After incubation, samples were separated by SDS-PAGE and methylation levels were examined by fluorography.

    Lysis:

    Article Title: Differential Effects of EGFR Ligands on Endocytic Sorting of the Receptor
    Article Snippet: EGFR content was then measured using an EGFR ELISA kit (RnD Systems). .. EGFR degradation analysis using 35 S methionine/cysteine labelling Serum-starved cells were pre-incubated in methionine- /cysteine-free DMEM supplemented with 1 mm sodium pyruvate, 2 mm glutamax, 200 U/mL penicillin, and 50 ng/mL streptomycin for 1 h. After preincubation, cells were labelled with 0.25 mCi/mL L- [35 S]-methionine/cysteine (Mpbiomedicals) for 1–2 h. Cells were changed to starvation medium and allowed to synthesize protein for 3 h. After 3 h cells were incubated with ligands for 1 h on ice, then washed in starvation medium, and incubated in starvation medium at 37°C for 0–6 h. At the indicated times, cells were rinsed in cold PBS and then scraped off in RIPA lysis buffer supplemented with Protease Inhibitor Cocktail Set II and Phosphatase Inhibitor Cocktail Set III. .. Cell debris was removed by spinning at 13,000 ×g ; the lysates were pre-cleared with protein G Dynabeads (Invitrogen), and EGFR immunoprecipitated with mouse monoclonal anti-EGFR antibody (clone 199.12) and protein G Dynabeads.

    Protease Inhibitor:

    Article Title: Differential Effects of EGFR Ligands on Endocytic Sorting of the Receptor
    Article Snippet: EGFR content was then measured using an EGFR ELISA kit (RnD Systems). .. EGFR degradation analysis using 35 S methionine/cysteine labelling Serum-starved cells were pre-incubated in methionine- /cysteine-free DMEM supplemented with 1 mm sodium pyruvate, 2 mm glutamax, 200 U/mL penicillin, and 50 ng/mL streptomycin for 1 h. After preincubation, cells were labelled with 0.25 mCi/mL L- [35 S]-methionine/cysteine (Mpbiomedicals) for 1–2 h. Cells were changed to starvation medium and allowed to synthesize protein for 3 h. After 3 h cells were incubated with ligands for 1 h on ice, then washed in starvation medium, and incubated in starvation medium at 37°C for 0–6 h. At the indicated times, cells were rinsed in cold PBS and then scraped off in RIPA lysis buffer supplemented with Protease Inhibitor Cocktail Set II and Phosphatase Inhibitor Cocktail Set III. .. Cell debris was removed by spinning at 13,000 ×g ; the lysates were pre-cleared with protein G Dynabeads (Invitrogen), and EGFR immunoprecipitated with mouse monoclonal anti-EGFR antibody (clone 199.12) and protein G Dynabeads.

    Titration:

    Article Title: Identification of immune genes and proteins involved in the response of bovine mammary tissue to Staphylococcus aureus infection
    Article Snippet: .. Challenge of bMEC with LPS and LTA The culture and characterisation of the primary bovine mammary epithelial cells (bMEC) have been described elsewhere [ ]. bMEC were grown in M199, Hams F12, 10 mM Hepes, 20 mM NaHCO3 , 2 mM NaC2 H3 O2 , 20% horse serum, 5% FCS, 100 U/ml penicillin, 100 μg/ml streptomycin, 250 ng/ml amphotericin B, 100 ng/ml kanamycin (all reagents from Invitrogen Corporation Carlsbad, California), 5 μg/ml insulin, 1 μg/ml cortisol (Sigma Chemical Co., St Louis), 10 ng/ml EGF (MP Biomedicals, Irvine, CA), pH 7.4. bMEC were seeded at 4 × 104 cells per well in 6 well plates (Sarstedt) and grown until 80% confluence at 37°C, then challenged with LPS (50 μg/ml) or LTA (20 μg/ml) for 0, 2, 4, 8, 16 and 24 h. The concentrations of LTA and LPS used with the bMEC were determined from titration analyses [ ]. .. LPS was purchased as a lyophilised powder purified from E. coli strain 055:B5 by phenol extraction (Sigma Chemical Co.) [ ].

    Methylation:

    Article Title: PRMT1-mediated methylation of the EGF receptor regulates signaling and cetuximab response
    Article Snippet: GST-tagged recombinant proteins were then purified using glutathione sepharose 4B (GE Healthcare) and dialyzed against phosphate-buffered saline. .. To perform in vitro methylation assay, GST-tagged recombinant PRMT1 and EGFR fragments were incubated together in the presence of 2.2 Ci S-adenosyl-L-[methyl-3 H] methionine (85 Ci/mmol from a 0.55 mCi/ml stock solution; MP Biomedicals) for 1 hour at 30°C in a final volume of 50 μl of phosphate-buffered saline. .. After incubation, samples were separated by SDS-PAGE and methylation levels were examined by fluorography.

    Recombinant:

    Article Title: PRMT1-mediated methylation of the EGF receptor regulates signaling and cetuximab response
    Article Snippet: GST-tagged recombinant proteins were then purified using glutathione sepharose 4B (GE Healthcare) and dialyzed against phosphate-buffered saline. .. To perform in vitro methylation assay, GST-tagged recombinant PRMT1 and EGFR fragments were incubated together in the presence of 2.2 Ci S-adenosyl-L-[methyl-3 H] methionine (85 Ci/mmol from a 0.55 mCi/ml stock solution; MP Biomedicals) for 1 hour at 30°C in a final volume of 50 μl of phosphate-buffered saline. .. After incubation, samples were separated by SDS-PAGE and methylation levels were examined by fluorography.

    TUNEL Assay:

    Article Title: Lipid phosphate phosphatase-1 expression in cancer cells attenuates tumor growth and metastasis in mice [S]
    Article Snippet: .. Other reagents were purchased as follows: epidermal growth factor (EGF) (CRE009B) (Cell Sciences, Canton, MA); doxycycline (0219895525) (MP Biomedicals, Solon, OH); mouse anti-phospho-Akt (4051S), rabbit anti-Akt (4691S), mouse anti-phospho-ERK (9106S), rabbit anti-ERK (9102S), and rabbit anti-Ki67 (12202S) antibodies (Cell Signaling Technologies, Danvers, MA); rabbit anti-CD31 (ab28364) antibody (Abcam, Toronto, Ontario, Canada); mouse anti-FLAG (F1804) antibody to the octapeptide, AspTyrLysAspAspAspAspLys, fatty acid-free BSA (A8806), 1,2-bis(o-aminophenoxy)ethane- N , N , N ’, N ’-tetraacetic acid (BAPTA)-AM (A1076), LY294002 (L9908), (U6756), (U6881), wortmannin (W1628), pertussis toxin (PTX) (P140), and probenecid (P8761) (Sigma, St. Louis, MO); PolyJet (SL100688) (SignaGen Laboratory, Gaithersburg, MD); G418 sulfate (11811-031), LR clonase enzyme mix (11791-019), Fura-2 AM (F-1201), and F127 (P-6867) (Life Technologies, Grand Island, NY); PfuUltra DNA polymerase (600385) (Agilent Technologies, Santa Clara, CA); restriction enzymes Spe I (R0133), Sal I (R0138), Xho I (R0146), and T4 DNA ligase (M0202) (New England Biolabs, Ipswich, MA); Matrigel (354230/354234) (BD Biosciences, San Jose, CA); terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay kit (APT110) (Millipore, Billerica, MA); and RhoA (BK036), Rac (BK035), and cdc42 (BK034) activation assay kits (Cytoskeleton, Denver, CO). .. [32 P]LPA and [32 P]sphingosine 1-phosphate (S1P) were prepared as described previously ( , ) and diluted with nonradioactive substrate to a specific radioactivity of 1 × 107 cpm/µmol.

    Activation Assay:

    Article Title: Lipid phosphate phosphatase-1 expression in cancer cells attenuates tumor growth and metastasis in mice [S]
    Article Snippet: .. Other reagents were purchased as follows: epidermal growth factor (EGF) (CRE009B) (Cell Sciences, Canton, MA); doxycycline (0219895525) (MP Biomedicals, Solon, OH); mouse anti-phospho-Akt (4051S), rabbit anti-Akt (4691S), mouse anti-phospho-ERK (9106S), rabbit anti-ERK (9102S), and rabbit anti-Ki67 (12202S) antibodies (Cell Signaling Technologies, Danvers, MA); rabbit anti-CD31 (ab28364) antibody (Abcam, Toronto, Ontario, Canada); mouse anti-FLAG (F1804) antibody to the octapeptide, AspTyrLysAspAspAspAspLys, fatty acid-free BSA (A8806), 1,2-bis(o-aminophenoxy)ethane- N , N , N ’, N ’-tetraacetic acid (BAPTA)-AM (A1076), LY294002 (L9908), (U6756), (U6881), wortmannin (W1628), pertussis toxin (PTX) (P140), and probenecid (P8761) (Sigma, St. Louis, MO); PolyJet (SL100688) (SignaGen Laboratory, Gaithersburg, MD); G418 sulfate (11811-031), LR clonase enzyme mix (11791-019), Fura-2 AM (F-1201), and F127 (P-6867) (Life Technologies, Grand Island, NY); PfuUltra DNA polymerase (600385) (Agilent Technologies, Santa Clara, CA); restriction enzymes Spe I (R0133), Sal I (R0138), Xho I (R0146), and T4 DNA ligase (M0202) (New England Biolabs, Ipswich, MA); Matrigel (354230/354234) (BD Biosciences, San Jose, CA); terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay kit (APT110) (Millipore, Billerica, MA); and RhoA (BK036), Rac (BK035), and cdc42 (BK034) activation assay kits (Cytoskeleton, Denver, CO). .. [32 P]LPA and [32 P]sphingosine 1-phosphate (S1P) were prepared as described previously ( , ) and diluted with nonradioactive substrate to a specific radioactivity of 1 × 107 cpm/µmol.

    shRNA:

    Article Title: RNA Interference Against Hepatic Epidermal Growth Factor Receptor Has Suppressive Effects on Liver Regeneration in Rats
    Article Snippet: Positive clones were confirmed by restriction digestion and sequencing. .. A mixture of two EGFR-specific endotoxin-free shRNA plasmids at 300 μg was diluted in 5% glucose and was complexed with linear Polyethylenimine ( In vivo JET PEI; Q-Biogene, Carlsbad, CA) at an N/P ratio of six, following the manufacturer’s protocol. .. The gene-specific shRNA/PEI complex was injected (in separate experiments) via the superior mesenteric vein 1 day before partial PHx.

    In Vivo:

    Article Title: RNA Interference Against Hepatic Epidermal Growth Factor Receptor Has Suppressive Effects on Liver Regeneration in Rats
    Article Snippet: Positive clones were confirmed by restriction digestion and sequencing. .. A mixture of two EGFR-specific endotoxin-free shRNA plasmids at 300 μg was diluted in 5% glucose and was complexed with linear Polyethylenimine ( In vivo JET PEI; Q-Biogene, Carlsbad, CA) at an N/P ratio of six, following the manufacturer’s protocol. .. The gene-specific shRNA/PEI complex was injected (in separate experiments) via the superior mesenteric vein 1 day before partial PHx.

    FLAG-tag:

    Article Title: RhoE Is Required for Keratinocyte Differentiation and Stratification
    Article Snippet: Western blots were carried out with equal amount of protein per sample (bicinchoninic acid; Pierce Chemical, Rockford, IL). .. Primary mouse monoclonal antibodies used were against: myc epitope (9E10; Cancer Research UK [CRUK], London, United Kingdom), hemagglutinin (HA) (262K; Cell Signaling Technology, Danvers, MA), and FLAG epitope (M2, Sigma Chemical), E-cadherin (HECD1), α3β1 integrin (VM-2), Rac (23A8; Millipore, Billerica, MA), RhoA (26C4; Santa Cruz Biotechnology, Santa Cruz, CA), RhoE (4; Millipore), involucrin (SY5; Sigma Chemical), vinculin (hVIN; Sigma Chemical), epidermal growth factor receptor (EGFR) (CRUK), actin (C4; MP Biomedicals, Irvine, CA), phospho-p44/42 mitogen-activated protein kinase (MAPK) 1/2 (Cell Signaling Technology), and 5-bromo-2′-deoxyuridine (BrdU) (BD Biosciences, San Jose, CA). .. Primary rat monoclonal antibodies used were anti-E-cadherin (ECCD-2; Invitrogen, Paisley, United Kingdom), and anti-myc epitope (JAC6; Serotec, Oxford, United Kingdom).

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  • 93
    Valiant egfr fragments
    <t>PRMT1</t> interacts with and methylates <t>EGFR</t> before it is transported to the cell membrane.
    Egfr Fragments, supplied by Valiant, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egfr fragments/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    egfr fragments - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    PRMT1 interacts with and methylates EGFR before it is transported to the cell membrane.

    Journal: The Journal of Clinical Investigation

    Article Title: PRMT1-mediated methylation of the EGF receptor regulates signaling and cetuximab response

    doi: 10.1172/JCI82826

    Figure Lengend Snippet: PRMT1 interacts with and methylates EGFR before it is transported to the cell membrane.

    Article Snippet: To perform in vitro methylation assay, GST-tagged recombinant PRMT1 and EGFR fragments were incubated together in the presence of 2.2 Ci S-adenosyl-L-[methyl-3 H] methionine (85 Ci/mmol from a 0.55 mCi/ml stock solution; MP Biomedicals) for 1 hour at 30°C in a final volume of 50 μl of phosphate-buffered saline.

    Techniques:

    PRMT1 upregulates EGFR signaling and cell proliferation in colorectal cancer cell lines.

    Journal: The Journal of Clinical Investigation

    Article Title: PRMT1-mediated methylation of the EGF receptor regulates signaling and cetuximab response

    doi: 10.1172/JCI82826

    Figure Lengend Snippet: PRMT1 upregulates EGFR signaling and cell proliferation in colorectal cancer cell lines.

    Article Snippet: To perform in vitro methylation assay, GST-tagged recombinant PRMT1 and EGFR fragments were incubated together in the presence of 2.2 Ci S-adenosyl-L-[methyl-3 H] methionine (85 Ci/mmol from a 0.55 mCi/ml stock solution; MP Biomedicals) for 1 hour at 30°C in a final volume of 50 μl of phosphate-buffered saline.

    Techniques:

    PRMT1 upregulates EGFR dimerization and activation, and EGFR-dependent cell proliferation through R198/200 methylation.

    Journal: The Journal of Clinical Investigation

    Article Title: PRMT1-mediated methylation of the EGF receptor regulates signaling and cetuximab response

    doi: 10.1172/JCI82826

    Figure Lengend Snippet: PRMT1 upregulates EGFR dimerization and activation, and EGFR-dependent cell proliferation through R198/200 methylation.

    Article Snippet: To perform in vitro methylation assay, GST-tagged recombinant PRMT1 and EGFR fragments were incubated together in the presence of 2.2 Ci S-adenosyl-L-[methyl-3 H] methionine (85 Ci/mmol from a 0.55 mCi/ml stock solution; MP Biomedicals) for 1 hour at 30°C in a final volume of 50 μl of phosphate-buffered saline.

    Techniques: Activation Assay, Methylation

    PRMT1 methylates EGFR at R198 and R200.

    Journal: The Journal of Clinical Investigation

    Article Title: PRMT1-mediated methylation of the EGF receptor regulates signaling and cetuximab response

    doi: 10.1172/JCI82826

    Figure Lengend Snippet: PRMT1 methylates EGFR at R198 and R200.

    Article Snippet: To perform in vitro methylation assay, GST-tagged recombinant PRMT1 and EGFR fragments were incubated together in the presence of 2.2 Ci S-adenosyl-L-[methyl-3 H] methionine (85 Ci/mmol from a 0.55 mCi/ml stock solution; MP Biomedicals) for 1 hour at 30°C in a final volume of 50 μl of phosphate-buffered saline.

    Techniques: