Journal: Fungal biology and biotechnology

Article Title: Isolation and characterization of extracellular vesicles from biotechnologically important fungus Aureobasidium pullulans.

doi: 10.1186/s40694-022-00146-7

Figure Lengend Snippet: Fig. 2 NTA detection of concentration and size distribution of A. pullulans EVs

Article Snippet: One loop of Aureobasidium pullulans culture was inoculated into 20 mL of liquid yeast nitrogen base (YNB) medium (pH 7.0) consisting of 0.17% yeast nitrogen base (Qbiogene, USA), 0.5% ammonium sulphate (SigmaAldrich, USA) and 2% glucose (Fisher Scientific, USA) in deionized water.

Techniques: Concentration Assay

Journal: Fungal biology and biotechnology

Article Title: Isolation and characterization of extracellular vesicles from biotechnologically important fungus Aureobasidium pullulans.

doi: 10.1186/s40694-022-00146-7

Figure Lengend Snippet: Fig. 1 TEM micrographs of isolated EVs from A. pullulans. Images show different subgroups of EVs. The yellow arrows indicate the extracellular vesicles

Article Snippet: One loop of Aureobasidium pullulans culture was inoculated into 20 mL of liquid yeast nitrogen base (YNB) medium (pH 7.0) consisting of 0.17% yeast nitrogen base (Qbiogene, USA), 0.5% ammonium sulphate (SigmaAldrich, USA) and 2% glucose (Fisher Scientific, USA) in deionized water.

Techniques: Isolation

Journal: Fungal biology and biotechnology

Article Title: Isolation and characterization of extracellular vesicles from biotechnologically important fungus Aureobasidium pullulans.

doi: 10.1186/s40694-022-00146-7

Figure Lengend Snippet: Fig. 3 A NTA detection of concentration and size of nanoparticles and (B) distribution of total EV proteins (µg) and melanin content (A400) in different EV fractions isolated from A. pullulans by sucrose density gradient ultracentrifugation

Article Snippet: One loop of Aureobasidium pullulans culture was inoculated into 20 mL of liquid yeast nitrogen base (YNB) medium (pH 7.0) consisting of 0.17% yeast nitrogen base (Qbiogene, USA), 0.5% ammonium sulphate (SigmaAldrich, USA) and 2% glucose (Fisher Scientific, USA) in deionized water.

Techniques: Concentration Assay, Isolation

Journal: Fungal biology and biotechnology

Article Title: Isolation and characterization of extracellular vesicles from biotechnologically important fungus Aureobasidium pullulans.

doi: 10.1186/s40694-022-00146-7

Figure Lengend Snippet: Fig. 4 The top 30 GO terms overrepresented in A. pullulans EVs proteins in A Molecular Function, and B Biological Process. Encrichment FDR is the false discovery rate value of the enrichment analysis. The full list can be found in Additional file 2

Article Snippet: One loop of Aureobasidium pullulans culture was inoculated into 20 mL of liquid yeast nitrogen base (YNB) medium (pH 7.0) consisting of 0.17% yeast nitrogen base (Qbiogene, USA), 0.5% ammonium sulphate (SigmaAldrich, USA) and 2% glucose (Fisher Scientific, USA) in deionized water.

Techniques:

Journal: Fungal biology and biotechnology

Article Title: Isolation and characterization of extracellular vesicles from biotechnologically important fungus Aureobasidium pullulans.

doi: 10.1186/s40694-022-00146-7

Figure Lengend Snippet: Fig. 7 Test of the biocontrol potential of EVs from A. pullulans on three pathogenic fungi B. cinerea, C. acutatum, and P. expansum. Fungi were inoculated in the center of PDA medium plates and incubated at 24 °C until the growth on plates was confluent (one week). Then, a fresh EVs sample (6.0 × 108 (SE ± 1.6 × 107) nanoparticles/mL of culture medium) was spotten on cultures in three replicates. Sterile DPBS was also spotted on cultures in three replicates as a control. After four days of incubation at 24 °C, the cultures were examined for changes. Scale bar represents 0.5 cm

Article Snippet: One loop of Aureobasidium pullulans culture was inoculated into 20 mL of liquid yeast nitrogen base (YNB) medium (pH 7.0) consisting of 0.17% yeast nitrogen base (Qbiogene, USA), 0.5% ammonium sulphate (SigmaAldrich, USA) and 2% glucose (Fisher Scientific, USA) in deionized water.

Techniques: Incubation, Sterility, Control

Journal: Fungal biology and biotechnology

Article Title: Isolation and characterization of extracellular vesicles from biotechnologically important fungus Aureobasidium pullulans.

doi: 10.1186/s40694-022-00146-7

Figure Lengend Snippet: Fig. 6 Results of testing the effect of A. pullulans EVs on spore germination of the phytopathogenic fungi B. cinerea, C. acutatum, and P. expansum. Fresh EVs sample (7.2 × 108 (SE ± 2.9 × 107) nanoparticles/mL of culture medium) was gently mixed with 1.5 × 105 spores/mL in DPBS and then inoculated into the center of the PDA medium plate. Sterile DPBS was used as a control instead of the EVs sample. The plates were incubated at 24 °C for five days and then the size of the colonies was measured to determine whether the growth of phytopathogenic strains was reduced. The plate diameter was 90 mm

Article Snippet: One loop of Aureobasidium pullulans culture was inoculated into 20 mL of liquid yeast nitrogen base (YNB) medium (pH 7.0) consisting of 0.17% yeast nitrogen base (Qbiogene, USA), 0.5% ammonium sulphate (SigmaAldrich, USA) and 2% glucose (Fisher Scientific, USA) in deionized water.

Techniques: Sterility, Control, Incubation