laminin (Valiant)
93
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Name:
Laminin
Description:
Laminin
Catalog Number:
0215002701
Price:
522.95
Category:
Life Sciences Biochemicals
Applications:
Cell adhesion factor, Supports growth and differentiation of many cell
Size:
1 mg
|
Buy from Supplier |
Structured Review
Valiant
laminin
Laminin
https://www.bioz.com/result/laminin/product/Valiant
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Laminin
https://www.bioz.com/result/laminin/product/Valiant
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
laminin - by Bioz Stars,
2021-03
93/100 stars
Images
1) Product Images from "Soluble VCAM-1 impairs human brain endothelial barrier integrity via integrin α-4-transduced outside-in signalling"
Article Title: Soluble VCAM-1 impairs human brain endothelial barrier integrity via integrin α-4-transduced outside-in signalling
Journal: Acta Neuropathologica
doi: 10.1007/s00401-015-1417-0
Figure Legend Snippet: Expression of brain endothelial integrins α-4, β-1 and β-7 in human active white matter lesions in post-mortem material of MS cases compared to controls without clinical and autoptic evidence for a pathological CNS condition. MS lesion activity was characterised by myelin staining against proteolipid protein and against HLA-DR to visualise mononuclear immune cells and activated microglia (data not shown). a Immunoreactivity for integrin α-4 (CD49d) is labelled in green , HLA-DR (clone LN3) in red to visualise immune cells and laminin in blue to visualise the vascular basement membrane. The right column shows merged images. b DAB bright field stainings against integrin α-4 of samples from different cases than those shown in a to demonstrate tissue preservation, relation of blood vessels to the surrounding tissue and regulation of integrin α-4 expression. c , d Expression of integrin β-1 ( c ) or integrin β-7 ( d ) in active MS lesions versus control tissue, as demonstrated by DAB bright field stainings. Representative of autoptic samples from supratentorial white matter of seven different MS cases and four controls (1 tissue block per case). Scale bars 10 µm ( a , b ) or 50 µm ( c , d )
Techniques Used: Expressing, Mass Spectrometry, Activity Assay, Staining, Preserving, Blocking Assay
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Cell Culture:Article Title: The Neurosteroid Allopregnanolone Promotes Proliferation of Rodent and Human Neural Progenitor Cells and Regulates Cell-Cycle Gene and Protein Expression Article Snippet: Human embryonic brain cortical stem cells (gift from Dr. Svendsen, Departments of Anatomy and Neurology and the Waisman Center, University of Wisconsin, Madison, WI) were provided as cryopreserved neurospheres. .. Neurospheres were cultured as described previously by Dr. Svendsen's laboratory ( ) in DMEM/Ham's F-12 medium (7:3) containing penicillin/streptomycin/amphotericin B (1%), supplemented with B27 (2%; Invitrogen, Gaithersburg, MD), epidermal growth factor (EGF) (20 ng/ml; Invitrogen, Gaithersburg, MD), FGF-2 (20 ng/ml; Invitrogen, Gaithersburg, MD), and heparin (5 μg/ml; Sigma, St. Louis, MO) in a humidified incubator (37°C and 5% CO2 ), and one-half of the growth medium was replenished every 3-4 d. Neurospheres were then mechanically triturated into single cells with flame-polished Pasteur pipettes, plated onto T75 flasks at a density equivalent to 2 × 106 cells per flask, and passaged every 14 d. After the second passage, cells were switched to maintenance media [DMEM/Ham's F-12 (7:3)] containing N2 supplement (1%; Invitrogen), 20 ng/ml EGF, and 10 ng/ml LIF (Chemicon, Temecula, CA) and seeded onto T75 flasks coated with Immunocytochemistry:Article Title: Mutations of EFHC1, linked to juvenile myoclonic epilepsy, disrupt radial and tangential migrations during brain development Article Snippet: For secondary antibodies, we used RRX-, FITC- or Cy5-conjugated antibodies to rabbit, mouse or rat IgG (Jackson ImmunoResearch, 1:500). .. For immunocytochemistry, cells were grown on glass coverslips coated with polyornithine (0.1 mg/ml, Sigma) for 1 h at room temperature and Immunofluorescence:Article Title: Simultaneous Gene Deletion of Gata4 and Gata6 Leads to Early Disruption of Follicular Development and Germ Cell Loss in the Murine Ovary 1 Article Snippet: .. Immunofluorescence experiments were carried out on 5–7 μm cryosections as previously described [ ], and the following primary antibodies were used: OCT3/4; GATA4, and AMH (1:300) from Santa Cruz Biotechnologies, MVH (1:300) from Abcam, FOXL2 (1:300; Everest Biotech), GATA6 (1:300; Cell Signaling), WT1 (1:500; Epitomics); SF1 (1:300; TransGenic, Inc.), phospho-Histone H3 (1:300; Millipore), SOHLH1 (1:300; a gift from Dr. Alex Rajkovic); SOX9 (1:300; Millipore); Transgenic Assay:Article Title: Simultaneous Gene Deletion of Gata4 and Gata6 Leads to Early Disruption of Follicular Development and Germ Cell Loss in the Murine Ovary 1 Article Snippet: .. Immunofluorescence experiments were carried out on 5–7 μm cryosections as previously described [ ], and the following primary antibodies were used: OCT3/4; GATA4, and AMH (1:300) from Santa Cruz Biotechnologies, MVH (1:300) from Abcam, FOXL2 (1:300; Everest Biotech), GATA6 (1:300; Cell Signaling), WT1 (1:500; Epitomics); SF1 (1:300; TransGenic, Inc.), phospho-Histone H3 (1:300; Millipore), SOHLH1 (1:300; a gift from Dr. Alex Rajkovic); SOX9 (1:300; Millipore); Staining:Article Title: Soluble VCAM-1 impairs human brain endothelial barrier integrity via integrin α-4-transduced outside-in signalling Article Snippet: For staining of integrin β-1, we used a rat monoclonal Ab (clone mAb13, BD Biosciences), and for integrin β-7, we employed the mouse monoclonal Ab FIB504 (BD Biosciences). .. Rabbit polyclonal Abs against von Willebrand factor (vWF; DAKO, Hamburg, Germany) or |
