laminin  (Valiant)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    Laminin
    Description:
    Laminin
    Catalog Number:
    0215002701
    Price:
    522.95
    Category:
    Life Sciences Biochemicals
    Applications:
    Cell adhesion factor, Supports growth and differentiation of many cell
    Size:
    1 mg
    Buy from Supplier


    Structured Review

    Valiant laminin
    Expression of brain endothelial integrins α-4, β-1 and β-7 in human active white matter lesions in post-mortem material of MS cases compared to controls without clinical and autoptic evidence for a pathological CNS condition. MS lesion activity was characterised by myelin staining against proteolipid protein and against HLA-DR to visualise mononuclear immune cells and activated microglia (data not shown). a Immunoreactivity for integrin α-4 (CD49d) is labelled in green , HLA-DR (clone LN3) in red to visualise immune cells and <t>laminin</t> in blue to visualise the vascular basement membrane. The right column shows merged images. b DAB bright field stainings against integrin α-4 of samples from different cases than those shown in a to demonstrate tissue preservation, relation of blood vessels to the surrounding tissue and regulation of integrin α-4 expression. c , d Expression of integrin β-1 ( c ) or integrin β-7 ( d ) in active MS lesions versus control tissue, as demonstrated by DAB bright field stainings. Representative of autoptic samples from supratentorial white matter of seven different MS cases and four controls (1 tissue block per case). Scale bars 10 µm ( a , b ) or 50 µm ( c , d )
    Laminin
    https://www.bioz.com/result/laminin/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    laminin - by Bioz Stars, 2021-03
    93/100 stars

    Images

    1) Product Images from "Soluble VCAM-1 impairs human brain endothelial barrier integrity via integrin α-4-transduced outside-in signalling"

    Article Title: Soluble VCAM-1 impairs human brain endothelial barrier integrity via integrin α-4-transduced outside-in signalling

    Journal: Acta Neuropathologica

    doi: 10.1007/s00401-015-1417-0

    Expression of brain endothelial integrins α-4, β-1 and β-7 in human active white matter lesions in post-mortem material of MS cases compared to controls without clinical and autoptic evidence for a pathological CNS condition. MS lesion activity was characterised by myelin staining against proteolipid protein and against HLA-DR to visualise mononuclear immune cells and activated microglia (data not shown). a Immunoreactivity for integrin α-4 (CD49d) is labelled in green , HLA-DR (clone LN3) in red to visualise immune cells and laminin in blue to visualise the vascular basement membrane. The right column shows merged images. b DAB bright field stainings against integrin α-4 of samples from different cases than those shown in a to demonstrate tissue preservation, relation of blood vessels to the surrounding tissue and regulation of integrin α-4 expression. c , d Expression of integrin β-1 ( c ) or integrin β-7 ( d ) in active MS lesions versus control tissue, as demonstrated by DAB bright field stainings. Representative of autoptic samples from supratentorial white matter of seven different MS cases and four controls (1 tissue block per case). Scale bars 10 µm ( a , b ) or 50 µm ( c , d )
    Figure Legend Snippet: Expression of brain endothelial integrins α-4, β-1 and β-7 in human active white matter lesions in post-mortem material of MS cases compared to controls without clinical and autoptic evidence for a pathological CNS condition. MS lesion activity was characterised by myelin staining against proteolipid protein and against HLA-DR to visualise mononuclear immune cells and activated microglia (data not shown). a Immunoreactivity for integrin α-4 (CD49d) is labelled in green , HLA-DR (clone LN3) in red to visualise immune cells and laminin in blue to visualise the vascular basement membrane. The right column shows merged images. b DAB bright field stainings against integrin α-4 of samples from different cases than those shown in a to demonstrate tissue preservation, relation of blood vessels to the surrounding tissue and regulation of integrin α-4 expression. c , d Expression of integrin β-1 ( c ) or integrin β-7 ( d ) in active MS lesions versus control tissue, as demonstrated by DAB bright field stainings. Representative of autoptic samples from supratentorial white matter of seven different MS cases and four controls (1 tissue block per case). Scale bars 10 µm ( a , b ) or 50 µm ( c , d )

    Techniques Used: Expressing, Mass Spectrometry, Activity Assay, Staining, Preserving, Blocking Assay

    Related Articles

    Cell Culture:

    Article Title: The Neurosteroid Allopregnanolone Promotes Proliferation of Rodent and Human Neural Progenitor Cells and Regulates Cell-Cycle Gene and Protein Expression
    Article Snippet: Human embryonic brain cortical stem cells (gift from Dr. Svendsen, Departments of Anatomy and Neurology and the Waisman Center, University of Wisconsin, Madison, WI) were provided as cryopreserved neurospheres. .. Neurospheres were cultured as described previously by Dr. Svendsen's laboratory ( ) in DMEM/Ham's F-12 medium (7:3) containing penicillin/streptomycin/amphotericin B (1%), supplemented with B27 (2%; Invitrogen, Gaithersburg, MD), epidermal growth factor (EGF) (20 ng/ml; Invitrogen, Gaithersburg, MD), FGF-2 (20 ng/ml; Invitrogen, Gaithersburg, MD), and heparin (5 μg/ml; Sigma, St. Louis, MO) in a humidified incubator (37°C and 5% CO2 ), and one-half of the growth medium was replenished every 3-4 d. Neurospheres were then mechanically triturated into single cells with flame-polished Pasteur pipettes, plated onto T75 flasks at a density equivalent to 2 × 106 cells per flask, and passaged every 14 d. After the second passage, cells were switched to maintenance media [DMEM/Ham's F-12 (7:3)] containing N2 supplement (1%; Invitrogen), 20 ng/ml EGF, and 10 ng/ml LIF (Chemicon, Temecula, CA) and seeded onto T75 flasks coated with laminin (MP Biomedicals, Irvine, CA) at a density of 2 × 106 cells per flask, which were then plated onto laminin-coated 96-well plates at a density of 7.5-15 × 104 cells per well or chamber slides at a density of 2-4 × 104 cells/cm2 before analysis. .. Immunocytochemical staining .

    Immunocytochemistry:

    Article Title: Mutations of EFHC1, linked to juvenile myoclonic epilepsy, disrupt radial and tangential migrations during brain development
    Article Snippet: For secondary antibodies, we used RRX-, FITC- or Cy5-conjugated antibodies to rabbit, mouse or rat IgG (Jackson ImmunoResearch, 1:500). .. For immunocytochemistry, cells were grown on glass coverslips coated with polyornithine (0.1 mg/ml, Sigma) for 1 h at room temperature and laminin (5 µg/ml, MP Biomedicals) for 2 h at 37°C. ..

    Immunofluorescence:

    Article Title: Simultaneous Gene Deletion of Gata4 and Gata6 Leads to Early Disruption of Follicular Development and Germ Cell Loss in the Murine Ovary 1
    Article Snippet: .. Immunofluorescence experiments were carried out on 5–7 μm cryosections as previously described [ ], and the following primary antibodies were used: OCT3/4; GATA4, and AMH (1:300) from Santa Cruz Biotechnologies, MVH (1:300) from Abcam, FOXL2 (1:300; Everest Biotech), GATA6 (1:300; Cell Signaling), WT1 (1:500; Epitomics); SF1 (1:300; TransGenic, Inc.), phospho-Histone H3 (1:300; Millipore), SOHLH1 (1:300; a gift from Dr. Alex Rajkovic); SOX9 (1:300; Millipore); Laminin (1:300; MP Biomedicals), and SPRR2 (1:100; Enzo Life Sciences). .. The tyramide signal amplification system (TSA; PerkinElmer Inc.) was used to amplify the signal of the SPRR2 antibody.

    Transgenic Assay:

    Article Title: Simultaneous Gene Deletion of Gata4 and Gata6 Leads to Early Disruption of Follicular Development and Germ Cell Loss in the Murine Ovary 1
    Article Snippet: .. Immunofluorescence experiments were carried out on 5–7 μm cryosections as previously described [ ], and the following primary antibodies were used: OCT3/4; GATA4, and AMH (1:300) from Santa Cruz Biotechnologies, MVH (1:300) from Abcam, FOXL2 (1:300; Everest Biotech), GATA6 (1:300; Cell Signaling), WT1 (1:500; Epitomics); SF1 (1:300; TransGenic, Inc.), phospho-Histone H3 (1:300; Millipore), SOHLH1 (1:300; a gift from Dr. Alex Rajkovic); SOX9 (1:300; Millipore); Laminin (1:300; MP Biomedicals), and SPRR2 (1:100; Enzo Life Sciences). .. The tyramide signal amplification system (TSA; PerkinElmer Inc.) was used to amplify the signal of the SPRR2 antibody.

    Staining:

    Article Title: Soluble VCAM-1 impairs human brain endothelial barrier integrity via integrin α-4-transduced outside-in signalling
    Article Snippet: For staining of integrin β-1, we used a rat monoclonal Ab (clone mAb13, BD Biosciences), and for integrin β-7, we employed the mouse monoclonal Ab FIB504 (BD Biosciences). .. Rabbit polyclonal Abs against von Willebrand factor (vWF; DAKO, Hamburg, Germany) or laminin (MP Biomedicals, Eindhoven, Netherlands) were used to stain the brain vasculature. .. A biotinylated antibody against HLA-DR (clone LN3) was used to stain immune cells.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Valiant laminin
    Expression of brain endothelial integrins α-4, β-1 and β-7 in human active white matter lesions in post-mortem material of MS cases compared to controls without clinical and autoptic evidence for a pathological CNS condition. MS lesion activity was characterised by myelin staining against proteolipid protein and against HLA-DR to visualise mononuclear immune cells and activated microglia (data not shown). a Immunoreactivity for integrin α-4 (CD49d) is labelled in green , HLA-DR (clone LN3) in red to visualise immune cells and <t>laminin</t> in blue to visualise the vascular basement membrane. The right column shows merged images. b DAB bright field stainings against integrin α-4 of samples from different cases than those shown in a to demonstrate tissue preservation, relation of blood vessels to the surrounding tissue and regulation of integrin α-4 expression. c , d Expression of integrin β-1 ( c ) or integrin β-7 ( d ) in active MS lesions versus control tissue, as demonstrated by DAB bright field stainings. Representative of autoptic samples from supratentorial white matter of seven different MS cases and four controls (1 tissue block per case). Scale bars 10 µm ( a , b ) or 50 µm ( c , d )
    Laminin, supplied by Valiant, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/laminin/product/Valiant
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    laminin - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    Image Search Results


    Expression of brain endothelial integrins α-4, β-1 and β-7 in human active white matter lesions in post-mortem material of MS cases compared to controls without clinical and autoptic evidence for a pathological CNS condition. MS lesion activity was characterised by myelin staining against proteolipid protein and against HLA-DR to visualise mononuclear immune cells and activated microglia (data not shown). a Immunoreactivity for integrin α-4 (CD49d) is labelled in green , HLA-DR (clone LN3) in red to visualise immune cells and laminin in blue to visualise the vascular basement membrane. The right column shows merged images. b DAB bright field stainings against integrin α-4 of samples from different cases than those shown in a to demonstrate tissue preservation, relation of blood vessels to the surrounding tissue and regulation of integrin α-4 expression. c , d Expression of integrin β-1 ( c ) or integrin β-7 ( d ) in active MS lesions versus control tissue, as demonstrated by DAB bright field stainings. Representative of autoptic samples from supratentorial white matter of seven different MS cases and four controls (1 tissue block per case). Scale bars 10 µm ( a , b ) or 50 µm ( c , d )

    Journal: Acta Neuropathologica

    Article Title: Soluble VCAM-1 impairs human brain endothelial barrier integrity via integrin α-4-transduced outside-in signalling

    doi: 10.1007/s00401-015-1417-0

    Figure Lengend Snippet: Expression of brain endothelial integrins α-4, β-1 and β-7 in human active white matter lesions in post-mortem material of MS cases compared to controls without clinical and autoptic evidence for a pathological CNS condition. MS lesion activity was characterised by myelin staining against proteolipid protein and against HLA-DR to visualise mononuclear immune cells and activated microglia (data not shown). a Immunoreactivity for integrin α-4 (CD49d) is labelled in green , HLA-DR (clone LN3) in red to visualise immune cells and laminin in blue to visualise the vascular basement membrane. The right column shows merged images. b DAB bright field stainings against integrin α-4 of samples from different cases than those shown in a to demonstrate tissue preservation, relation of blood vessels to the surrounding tissue and regulation of integrin α-4 expression. c , d Expression of integrin β-1 ( c ) or integrin β-7 ( d ) in active MS lesions versus control tissue, as demonstrated by DAB bright field stainings. Representative of autoptic samples from supratentorial white matter of seven different MS cases and four controls (1 tissue block per case). Scale bars 10 µm ( a , b ) or 50 µm ( c , d )

    Article Snippet: Rabbit polyclonal Abs against von Willebrand factor (vWF; DAKO, Hamburg, Germany) or laminin (MP Biomedicals, Eindhoven, Netherlands) were used to stain the brain vasculature.

    Techniques: Expressing, Mass Spectrometry, Activity Assay, Staining, Preserving, Blocking Assay

    Rho1 knockdown causes wrapping defects in the optic stalk. A – B) Optic stalks of (A) control ( repo > Dcr-2 > sty -Z) and (B) Rho1 RNAi ( repo > Dcr-2 > sty -Z > Rho1 RNAi #29002) labelled with β-galactosidase to detect sprouty -LacZ ( sty -Z; green). C) Control ( repo 4.3 > CD8GFP > LacZ) and (D) Rho1 RNAi ( repo 4.3 > CD8GFP > Rho1 RNAi). Repo > CD8GFP is shown in red (C’ and D’) and photoreceptor axons are stained by Hrp and shown in grey (C’’ and D’’). (E, G and I) Control (repo > Dcr-2 > LacZ) and (F, H and J) Rho1 RNAi ( repo > Dcr-2 > Rho1 RNAi) transversal cut of the OS. Laminin A (LanA) is shown in green and glia nuclei (Repo) and membranes (Repo > CD8GFP) in red. Hrp stains photoreceptor axons in grey. Transversal cut of the OS analysis by TEM from (K and M) Control ( repo > Dcr-2 > LacZ) and (L and N) Rho1 RNAi ( repo > Dcr-2 > Rho1 RNAi). Wrapping glia (blue) enwraps each R1-R8 ommatidia laying apical to subperineurial glia (SPG; green). Perineurial glial cells (PG; lilac) form a layer externally to the SPG. In Rho1 RNAi instead of PG and SPG layer, only a single glia layer is visible (SPG-like in pink). Glia (repo) is shown in red. (A – J) Scale bars correspond to 10 μM. (K – N) Scale bars correspond to 1 μm.

    Journal: bioRxiv

    Article Title: The Pebble/Rho1/Anillin pathway controls polyploidization and axonal wrapping activity in the glial cells of the Drosophila eye

    doi: 10.1101/2020.02.12.945600

    Figure Lengend Snippet: Rho1 knockdown causes wrapping defects in the optic stalk. A – B) Optic stalks of (A) control ( repo > Dcr-2 > sty -Z) and (B) Rho1 RNAi ( repo > Dcr-2 > sty -Z > Rho1 RNAi #29002) labelled with β-galactosidase to detect sprouty -LacZ ( sty -Z; green). C) Control ( repo 4.3 > CD8GFP > LacZ) and (D) Rho1 RNAi ( repo 4.3 > CD8GFP > Rho1 RNAi). Repo > CD8GFP is shown in red (C’ and D’) and photoreceptor axons are stained by Hrp and shown in grey (C’’ and D’’). (E, G and I) Control (repo > Dcr-2 > LacZ) and (F, H and J) Rho1 RNAi ( repo > Dcr-2 > Rho1 RNAi) transversal cut of the OS. Laminin A (LanA) is shown in green and glia nuclei (Repo) and membranes (Repo > CD8GFP) in red. Hrp stains photoreceptor axons in grey. Transversal cut of the OS analysis by TEM from (K and M) Control ( repo > Dcr-2 > LacZ) and (L and N) Rho1 RNAi ( repo > Dcr-2 > Rho1 RNAi). Wrapping glia (blue) enwraps each R1-R8 ommatidia laying apical to subperineurial glia (SPG; green). Perineurial glial cells (PG; lilac) form a layer externally to the SPG. In Rho1 RNAi instead of PG and SPG layer, only a single glia layer is visible (SPG-like in pink). Glia (repo) is shown in red. (A – J) Scale bars correspond to 10 μM. (K – N) Scale bars correspond to 1 μm.

    Article Snippet: Primary antibodies used were: mouse anti-repo antibody at 1:5 (8D12 anti-Repo, Developmental Studies Hybridoma Bank, DSHB), rat anti-repo at 1:1000 (a gift from Dr. Benjamin Altenhein, Institut für Genetik, Germany), rabbit anti-repo at 1:2000 (a gift from Dr. Benjamin Altenhein), rabbit anti-pH3 antibody at 1:1000 (Upstate), rat anti-Elav 1:100 (7E8A10 DSHB), goat anti-HRP antibody Cy5-conjugated at 1:100 (Jackson ImmunoResearch), rabbit anti-cleaved Caspase-3 at 1:200 (9661, Cell Signaling), rabbit anti-β-galactosidase antibody 1:2000 (Cappel, 55976, MP Biomedicals), rabbit anti-Laminin A antibody at 1:200 (a gift from Herwig O. Gutzeit) and mouse anti-Rho1 at 1:100 (p1D9, DSHB).Appropriate Alexa Fluor conjugated secondary antibodies used were from Molecular Probes.

    Techniques: Staining, Transmission Electron Microscopy

    Pax3 expression in Pax7 lacZ/lac Z and Pax7 +/lacZ musculature. (A–E and G–K) Triple staining of adult EDL muscle sections with laminin, Pax7, and Pax3 antibodies plus DAPI labeling of nuclei. Pax3 + cells (arrows), located in the interstitial space outside the basal lamina of myofibers, exist in similar frequency in both Pax7 + /lacZ (C) and Pax7 lacZ/lacZ (D) muscles. Note that the Pax7 + cell (arrowheads) in the Pax7 + /lacZ is located in a sublaminar position. Bar, 10 μm. (F and L) Pax3 in situ hybridization on Pax7 + /lacZ (F) and Pax7 lacZ/lacZ (L) diaphragm also revealed the presence of Pax3 + cells in both genotypes. Bar, 25 μm.

    Journal: The Journal of Cell Biology

    Article Title: Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis

    doi: 10.1083/jcb.200508001

    Figure Lengend Snippet: Pax3 expression in Pax7 lacZ/lac Z and Pax7 +/lacZ musculature. (A–E and G–K) Triple staining of adult EDL muscle sections with laminin, Pax7, and Pax3 antibodies plus DAPI labeling of nuclei. Pax3 + cells (arrows), located in the interstitial space outside the basal lamina of myofibers, exist in similar frequency in both Pax7 + /lacZ (C) and Pax7 lacZ/lacZ (D) muscles. Note that the Pax7 + cell (arrowheads) in the Pax7 + /lacZ is located in a sublaminar position. Bar, 10 μm. (F and L) Pax3 in situ hybridization on Pax7 + /lacZ (F) and Pax7 lacZ/lacZ (L) diaphragm also revealed the presence of Pax3 + cells in both genotypes. Bar, 25 μm.

    Article Snippet: The primary antibodies used were as follows: mouse monoclonal anti-Pax3 (IgG2a; a gift from C. Ordhal, University of California, San Francisco, San Francisco, CA; available from the Developmental Studies Hybridoma Bank), rabbit anti-Pax3 (Active Motif and Zymed Laboratories), Pax7 (Developmental Studies Hybridoma Bank), rat anti-laminin (Qbiogene), laminin (DakoCytomation), Desmin (DakoCytomation), CD34 (BD Biosciences), MyoD (5.8A; BD Biosciences), rabbit anti-MyoD (C-20; Santa Cruz Biotechnology, Inc.), Myf5 (Santa-Cruz Biotechnology, Inc.), MyHC (MF-20; Developmental Studies Hybridoma Bank), embryonic fast MyHC (F1.652; Developmental Studies Hybridoma Bank); MyHC IIb (BF-F3; Deutsche Sammlung von Mikroorganismen und Zellkulturen), MyHC I (A4.840) and IIa (A4.74; Developmental Studies Hybridoma Bank), anti–β-gal (Invitrogen), and chicken anti-Syndecan4 (gift of B. Olwin, University of Colorado, Boulder, CO; ).

    Techniques: Expressing, Staining, Labeling, In Situ Hybridization