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actinomycin d  (Valiant Co Ltd)
93
Valiant Co Ltd actinomycin d
A – D Expression of Il-12 , Cxcl11 , and Ifnb1 mRNA were measured by qRT-PCR analysis at 3 h following treatment of WT and Ifnar1 −/− BMDMs with LPS (1 ng/mL) and EMR (10 µM). E , F Expression of Tnfa mRNA was measured by qRT-PCR analysis at various time intervals post treatment of WT and Ifnar1 −/− BMDMs with LPS (1 ng/mL), EMR (10 µM), and IFNβ (10 ng/mL). G , H Expression of TNFα was measured by ELISA in the supernatants collected at different time intervals following treatment of WT and Ifnar1 −/− BMDMs with LPS (1 ng/mL), EMR (10 µM), and IFNβ (10 ng/mL), or <t>Actinomycin</t> <t>D</t> (2 µM). Each experiment was at least repeated three times. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).
Actinomycin D, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/actinomycin d/product/Valiant Co Ltd
Average 93 stars, based on 1 article reviews
actinomycin d - by Bioz Stars, 2026-02
93/100 stars
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transcriptional inhibitor actinomycin d  (Valiant Co Ltd)
93
Valiant Co Ltd transcriptional inhibitor actinomycin d
A – D Expression of Il-12 , Cxcl11 , and Ifnb1 mRNA were measured by qRT-PCR analysis at 3 h following treatment of WT and Ifnar1 −/− BMDMs with LPS (1 ng/mL) and EMR (10 µM). E , F Expression of Tnfa mRNA was measured by qRT-PCR analysis at various time intervals post treatment of WT and Ifnar1 −/− BMDMs with LPS (1 ng/mL), EMR (10 µM), and IFNβ (10 ng/mL). G , H Expression of TNFα was measured by ELISA in the supernatants collected at different time intervals following treatment of WT and Ifnar1 −/− BMDMs with LPS (1 ng/mL), EMR (10 µM), and IFNβ (10 ng/mL), or <t>Actinomycin</t> <t>D</t> (2 µM). Each experiment was at least repeated three times. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).
Transcriptional Inhibitor Actinomycin D, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transcriptional inhibitor actinomycin d/product/Valiant Co Ltd
Average 93 stars, based on 1 article reviews
transcriptional inhibitor actinomycin d - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier

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A – D Expression of Il-12 , Cxcl11 , and Ifnb1 mRNA were measured by qRT-PCR analysis at 3 h following treatment of WT and Ifnar1 −/− BMDMs with LPS (1 ng/mL) and EMR (10 µM). E , F Expression of Tnfa mRNA was measured by qRT-PCR analysis at various time intervals post treatment of WT and Ifnar1 −/− BMDMs with LPS (1 ng/mL), EMR (10 µM), and IFNβ (10 ng/mL). G , H Expression of TNFα was measured by ELISA in the supernatants collected at different time intervals following treatment of WT and Ifnar1 −/− BMDMs with LPS (1 ng/mL), EMR (10 µM), and IFNβ (10 ng/mL), or Actinomycin D (2 µM). Each experiment was at least repeated three times. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Journal: Cell Death & Disease

Article Title: Regulation of Zfp36 by ISGF3 and MK2 restricts the expression of inflammatory cytokines during necroptosis stimulation

doi: 10.1038/s41419-024-06964-4

Figure Lengend Snippet: A – D Expression of Il-12 , Cxcl11 , and Ifnb1 mRNA were measured by qRT-PCR analysis at 3 h following treatment of WT and Ifnar1 −/− BMDMs with LPS (1 ng/mL) and EMR (10 µM). E , F Expression of Tnfa mRNA was measured by qRT-PCR analysis at various time intervals post treatment of WT and Ifnar1 −/− BMDMs with LPS (1 ng/mL), EMR (10 µM), and IFNβ (10 ng/mL). G , H Expression of TNFα was measured by ELISA in the supernatants collected at different time intervals following treatment of WT and Ifnar1 −/− BMDMs with LPS (1 ng/mL), EMR (10 µM), and IFNβ (10 ng/mL), or Actinomycin D (2 µM). Each experiment was at least repeated three times. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: Actinomycin-D was obtained from MP Biomedicals (#02194525-CF).

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay