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( A ) Schematic of worms on plates with food (left), no food (middle), and no food with a barrier/fence (right). ( B ) Representative images of <t>palmitic</t> <t>acid</t> (PA), polyethylene glycol (PEG), and copper fences (edge of barrier is shown by an arrow) placed on the edge of NGM plates. Note: PEG is not visible when it dries. ( C ) Representative bright-field images of wildtype fed worms following a 96-hour exposure to PA, PEG 2,000, PEG 20,000, and copper as compared to a no fence control. ( D ) Quantification of images in (D) to determine the effect of each <t>fencing</t> tool on worm size. ( E ) Flee rate of day 1 adult wildtype worms under 24 hours of fed and fasted conditions with PA and PEG 20,000 fences as compared to a no fence control. ( F ) Flee rate of day 1 adult wildtype worms after a 24-hour exposure to pathogenic bacteria Enterococcus faecalis with PA and PEG 20,000 fences as compared to a no fence control. ( G ) Pharyngeal pumping and ( H ) thrashing rates of wildtype worms after a 24-hour exposure to PA and PEG 20,000 as compared to no fence fed control. ( I ) Lifespan of wildtype fed and DR (bacterial deprivation, or BD) worms at 20°C with PA and PEG 20,000 fences as compared to a no fence control. ( J ) Quantification of flee rate during lifespans shown in (I). One-way ANOVA with Tukey Post-Hoc analysis was used to derive p-values for worm size, flee rate, pumping and thrashing comparisons. Log-rank test was used to derive p-value for lifespan comparisons. All error bars shown in the figures represent the standard error of the mean (SEM). ns, not significant, * denotes p-value < 0.05, ** denotes p-value < 0.01, *** denotes p-value <0.001 and **** denotes p-value < 0.0001.
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( A ) Schematic of worms on plates with food (left), no food (middle), and no food with a barrier/fence (right). ( B ) Representative images of palmitic acid (PA), polyethylene glycol (PEG), and copper fences (edge of barrier is shown by an arrow) placed on the edge of NGM plates. Note: PEG is not visible when it dries. ( C ) Representative bright-field images of wildtype fed worms following a 96-hour exposure to PA, PEG 2,000, PEG 20,000, and copper as compared to a no fence control. ( D ) Quantification of images in (D) to determine the effect of each fencing tool on worm size. ( E ) Flee rate of day 1 adult wildtype worms under 24 hours of fed and fasted conditions with PA and PEG 20,000 fences as compared to a no fence control. ( F ) Flee rate of day 1 adult wildtype worms after a 24-hour exposure to pathogenic bacteria Enterococcus faecalis with PA and PEG 20,000 fences as compared to a no fence control. ( G ) Pharyngeal pumping and ( H ) thrashing rates of wildtype worms after a 24-hour exposure to PA and PEG 20,000 as compared to no fence fed control. ( I ) Lifespan of wildtype fed and DR (bacterial deprivation, or BD) worms at 20°C with PA and PEG 20,000 fences as compared to a no fence control. ( J ) Quantification of flee rate during lifespans shown in (I). One-way ANOVA with Tukey Post-Hoc analysis was used to derive p-values for worm size, flee rate, pumping and thrashing comparisons. Log-rank test was used to derive p-value for lifespan comparisons. All error bars shown in the figures represent the standard error of the mean (SEM). ns, not significant, * denotes p-value < 0.05, ** denotes p-value < 0.01, *** denotes p-value <0.001 and **** denotes p-value < 0.0001.

Journal: microPublication Biology

Article Title: Polyethylene glycol as an improved barrier to prevent fleeing in C. elegans

doi: 10.17912/micropub.biology.001288

Figure Lengend Snippet: ( A ) Schematic of worms on plates with food (left), no food (middle), and no food with a barrier/fence (right). ( B ) Representative images of palmitic acid (PA), polyethylene glycol (PEG), and copper fences (edge of barrier is shown by an arrow) placed on the edge of NGM plates. Note: PEG is not visible when it dries. ( C ) Representative bright-field images of wildtype fed worms following a 96-hour exposure to PA, PEG 2,000, PEG 20,000, and copper as compared to a no fence control. ( D ) Quantification of images in (D) to determine the effect of each fencing tool on worm size. ( E ) Flee rate of day 1 adult wildtype worms under 24 hours of fed and fasted conditions with PA and PEG 20,000 fences as compared to a no fence control. ( F ) Flee rate of day 1 adult wildtype worms after a 24-hour exposure to pathogenic bacteria Enterococcus faecalis with PA and PEG 20,000 fences as compared to a no fence control. ( G ) Pharyngeal pumping and ( H ) thrashing rates of wildtype worms after a 24-hour exposure to PA and PEG 20,000 as compared to no fence fed control. ( I ) Lifespan of wildtype fed and DR (bacterial deprivation, or BD) worms at 20°C with PA and PEG 20,000 fences as compared to a no fence control. ( J ) Quantification of flee rate during lifespans shown in (I). One-way ANOVA with Tukey Post-Hoc analysis was used to derive p-values for worm size, flee rate, pumping and thrashing comparisons. Log-rank test was used to derive p-value for lifespan comparisons. All error bars shown in the figures represent the standard error of the mean (SEM). ns, not significant, * denotes p-value < 0.05, ** denotes p-value < 0.01, *** denotes p-value <0.001 and **** denotes p-value < 0.0001.

Article Snippet: Fencing interventions Palmitic acid (PA): 0.5 g palmitic acid (MP Biomedicals 100905) was dissolved in 50 mL of 100% ethanol.

Techniques: Control, Bacteria