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n acetyl l cysteine  (Valiant Co Ltd)
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N Acetyl L Cysteine, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n acetylcysteine  (Valiant Co Ltd)
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Effect on co-immunoprecipitation of HER2 and HER4 caused by GRP alone, GRP + NAc, and GRP plus Tiron in NCI-H661 cells. ( A ) NCI-H661 cells were treated with N <t>-Acetylcysteine</t> (10 mM) or Tiron (5 mM). After 30 min, the cells were stimulated with GRP (0.1 μM) for 10 min and extracted with Lysis buffer. The extracts were immunoprecipitated with either anti-HER4 (top) or anti-HER2 Ab (bottom) overnight in the presence of protein A agarose. The beads were washed with PBS, treated with sample buffer, and the supernatant analyzed by Western blot for P-HER4 presence. ( B ) The % P-HER4 was determined for extracts immunoprecipitated with anti-HER4 and for extracts immunoprecipitated with anti-HER2. The mean value ± S.E. of 3 experiments is indicated; **, p < 0.01 vs. None; #, p < 0.05 vs. GRP. The exact p -values are shown in the text in 3.6. HER4 dimerization and the effect of ROS inhibitors section of the paper. Abbreviations: GRP, Gastrin-Releasing Peptide; NAc, N -acetylcysteine; P-HER4, PY1284-HER4; Tir, Tiron. The original western blot images can be found in .
N Acetylcysteine, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/n acetylcysteine/product/Valiant Co Ltd
Average 95 stars, based on 1 article reviews
n acetylcysteine - by Bioz Stars, 2026-02
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monash bdi organoid program recombinant human egf lonza peprotech af 100 15 n acetyl l cysteine nac  (Valiant Co Ltd)
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Valiant Co Ltd monash bdi organoid program recombinant human egf lonza peprotech af 100 15 n acetyl l cysteine nac
Effect on co-immunoprecipitation of HER2 and HER4 caused by GRP alone, GRP + NAc, and GRP plus Tiron in NCI-H661 cells. ( A ) NCI-H661 cells were treated with N <t>-Acetylcysteine</t> (10 mM) or Tiron (5 mM). After 30 min, the cells were stimulated with GRP (0.1 μM) for 10 min and extracted with Lysis buffer. The extracts were immunoprecipitated with either anti-HER4 (top) or anti-HER2 Ab (bottom) overnight in the presence of protein A agarose. The beads were washed with PBS, treated with sample buffer, and the supernatant analyzed by Western blot for P-HER4 presence. ( B ) The % P-HER4 was determined for extracts immunoprecipitated with anti-HER4 and for extracts immunoprecipitated with anti-HER2. The mean value ± S.E. of 3 experiments is indicated; **, p < 0.01 vs. None; #, p < 0.05 vs. GRP. The exact p -values are shown in the text in 3.6. HER4 dimerization and the effect of ROS inhibitors section of the paper. Abbreviations: GRP, Gastrin-Releasing Peptide; NAc, N -acetylcysteine; P-HER4, PY1284-HER4; Tir, Tiron. The original western blot images can be found in .
Monash Bdi Organoid Program Recombinant Human Egf Lonza Peprotech Af 100 15 N Acetyl L Cysteine Nac, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monash bdi organoid program recombinant human egf lonza peprotech af 100 15 n acetyl l cysteine nac/product/Valiant Co Ltd
Average 93 stars, based on 1 article reviews
monash bdi organoid program recombinant human egf lonza peprotech af 100 15 n acetyl l cysteine nac - by Bioz Stars, 2026-02
93/100 stars
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Effect on co-immunoprecipitation of HER2 and HER4 caused by GRP alone, GRP + NAc, and GRP plus Tiron in NCI-H661 cells. ( A ) NCI-H661 cells were treated with N -Acetylcysteine (10 mM) or Tiron (5 mM). After 30 min, the cells were stimulated with GRP (0.1 μM) for 10 min and extracted with Lysis buffer. The extracts were immunoprecipitated with either anti-HER4 (top) or anti-HER2 Ab (bottom) overnight in the presence of protein A agarose. The beads were washed with PBS, treated with sample buffer, and the supernatant analyzed by Western blot for P-HER4 presence. ( B ) The % P-HER4 was determined for extracts immunoprecipitated with anti-HER4 and for extracts immunoprecipitated with anti-HER2. The mean value ± S.E. of 3 experiments is indicated; **, p < 0.01 vs. None; #, p < 0.05 vs. GRP. The exact p -values are shown in the text in 3.6. HER4 dimerization and the effect of ROS inhibitors section of the paper. Abbreviations: GRP, Gastrin-Releasing Peptide; NAc, N -acetylcysteine; P-HER4, PY1284-HER4; Tir, Tiron. The original western blot images can be found in .

Journal: Biology

Article Title: Gastrin-Releasing Peptide Receptors Stimulate MAPK-Mediated Growth of Lung Cancer Cells by Transactivating HER4 in a Neuregulin-1, MAP Kinase-Dependent Manner Requiring Activation of the ROS-System

doi: 10.3390/biology14091225

Figure Lengend Snippet: Effect on co-immunoprecipitation of HER2 and HER4 caused by GRP alone, GRP + NAc, and GRP plus Tiron in NCI-H661 cells. ( A ) NCI-H661 cells were treated with N -Acetylcysteine (10 mM) or Tiron (5 mM). After 30 min, the cells were stimulated with GRP (0.1 μM) for 10 min and extracted with Lysis buffer. The extracts were immunoprecipitated with either anti-HER4 (top) or anti-HER2 Ab (bottom) overnight in the presence of protein A agarose. The beads were washed with PBS, treated with sample buffer, and the supernatant analyzed by Western blot for P-HER4 presence. ( B ) The % P-HER4 was determined for extracts immunoprecipitated with anti-HER4 and for extracts immunoprecipitated with anti-HER2. The mean value ± S.E. of 3 experiments is indicated; **, p < 0.01 vs. None; #, p < 0.05 vs. GRP. The exact p -values are shown in the text in 3.6. HER4 dimerization and the effect of ROS inhibitors section of the paper. Abbreviations: GRP, Gastrin-Releasing Peptide; NAc, N -acetylcysteine; P-HER4, PY1284-HER4; Tir, Tiron. The original western blot images can be found in .

Article Snippet: N -acetylcysteine was from MP Biomedicals (Santa Ana, CA, USA).

Techniques: Immunoprecipitation, Lysis, Western Blot