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Figure 1. Morphology and flow cytometry investigations of THP-1 monocyte differentiation into M0 and iDCs. (A–C) Light microscopy images. (D–F) Flow cytometry plots (forward scatter (FSC-A(/side scatter (SSC-A)). M0 and immature dendritic-like cells (iDCs)show increased size and granularity relative to THP-1 cells. (G,H) Surface marker expression in M0 and iDCs: (G) flow cytometry plot and confocal microscopy image of M0, showing CD14+ expression indicated by the data plot, as well as the red CD14 signal in the microscopic image; scale bar = 20 µm (H) flow cytometry plot of iDCs indicating a CD209+ signal, confirmed by the green signal (CD209) in the confocal microscopy image; scale bar = 20 µm.

Journal: International journal of molecular sciences

Article Title: Immune Modulation with Nanodiscs: Surface Charge Dictates Cellular Interactions and Activation of Macrophages and Dendritic-like Cells.

doi: 10.3390/ijms26115154

Figure Lengend Snippet: Figure 1. Morphology and flow cytometry investigations of THP-1 monocyte differentiation into M0 and iDCs. (A–C) Light microscopy images. (D–F) Flow cytometry plots (forward scatter (FSC-A(/side scatter (SSC-A)). M0 and immature dendritic-like cells (iDCs)show increased size and granularity relative to THP-1 cells. (G,H) Surface marker expression in M0 and iDCs: (G) flow cytometry plot and confocal microscopy image of M0, showing CD14+ expression indicated by the data plot, as well as the red CD14 signal in the microscopic image; scale bar = 20 µm (H) flow cytometry plot of iDCs indicating a CD209+ signal, confirmed by the green signal (CD209) in the confocal microscopy image; scale bar = 20 µm.

Article Snippet: Surface markers, i.e., Alexa Fluor 647 anti-human CD14 (M0) and FITC anti-human CD209 antibody (iDCs), were then added and incubated at 4 ◦C for a further 30 min. After a subsequent wash and centrifugation step, the mean fluorescence intensity (MFI) was measured using Cytek Aurora (Cytek Biosciences, Fremont, CA, USA) and Cytek SpectroFlow® 3.3.0 software.

Techniques: Flow Cytometry, Light Microscopy, Marker, Expressing, Confocal Microscopy