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Journal: Materials Today Bio
Article Title: Structurally reprogrammed modified citrus pectin (MCP) enables potentiated galectin-3 sequestration and injectable carboxymethyl chitosan/berberine hydrogel construction for osteoarthritis immunotherapy
doi: 10.1016/j.mtbio.2025.102330
Figure Lengend Snippet: Structural characterization and mechanical, injectable, and degradation properties of oxMCP/NOCC/BBR hydrogels. (A) Texture profile analysis (TPA) of the hydrogel's compressive properties ( n = 3). (B) Swelling ratios and (C) degradation profiles of hydrogels in RPMI-1640 medium at 37 °C over time ( n = 3). (D) XRD patterns of oxMCP, NOCC, BBR, and the corresponding hydrogels. (E) FTIR spectra of hydrogel components and composites. (F) Photographs showing hydrogel injectability through a 26 G needle and shape retention after extrusion. (G) Step-strain rheological test assessing the self-healing behaviors of the oxMCP/NOCC and oxMCP/NOCC/BBR hydrogels. (H) Confocal imaging of oxMCP/NOCC/BBR hydrogels labeled with Cy7-oxMCP (red) incubated with RITC-Gal-3 or RITC-BSA (green, 25 μg/mL) at 37 °C for 2 h. Scale bars: 20 μm. (I) Gal-3 adsorption kinetics of oxMCP/NOCC/BBR hydrogels at varying concentrations over 2 h.
Article Snippet: Recombinant human Gal-3 (ProSpec, Rehovot, Israel; CYT-606) and bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, USA) were fluorescently labeled with
Techniques: Imaging, Labeling, Incubation, Adsorption