Journal: Cell Death & Disease

Article Title: High glucose levels promote glycolysis and cholesterol synthesis via ERRα and suppress the autophagy–lysosomal pathway in endometrial cancer

doi: 10.1038/s41419-025-07499-y

Figure Lengend Snippet: A In the HEC-1A and KLE cells, the expression of HK2, p62 treated with 2-DG after ERRα overexpressing in the HG environment was shown by immunofluorescence (Magnification: 80×). B In the HEC-1A and KLE cells, the expression HMGCS1, p62 treated with simvastatin was presented by immunofluorescence staining after ERRα up-regulating in the HG environment (Magnification: 80×). C Relative fluorescence intensity of ERRα, HK2, and p62 when treated with 10 mM 2-DG for 24 h under high glucose in HEC-1A and KLE cells. D Relative fluorescence intensity of ERRα, HMGCS1, and p62 when treated with 5 μM and 10 μM simvastatin for 24 h under high glucose in HEC-1A and KLE cells, respectively. E , F TEM images of ASSs treated with 2-DG or simvastatin for 24 h under high glucose in KLE and HEC-1A cells, respectively (Magnification: 8000×). G , H The ASSs counts of HEC-1A and KLE cells treated with 2-DG or simvastatin for 24 h under high glucose after ERRα overexpressed, respectively. n = three independent experiments, * P < 0.05, ** P < 0.01 or *** P < 0.001. EC endometrial cancer, Mnt mannitol, NG normal glucose, HG high glucose, TEM transmission electron microscopy, 2-DG 2-deoxy- d -arabino-hexose.

Article Snippet: HEC-1A and KLE cells were incubated with 5 µM and 10 µM simvastatin (indole dimethyl sulfoxide [DMSO]) (#Y190601, MP Biomedicals LLC, CA, USA) for 24 h, they were treated with 10 mM 2-deoxy-D-glucose (2-DG) (#HY-13966, MedChemExpress LLC, NJ, USA) for 24 h, or with 10 μM 3-bromopyruvic acid (3-BrPA) (#HY-19992, MedChemExpress LLC, NJ, USA) for 24 h.

Techniques: Expressing, Immunofluorescence, Staining, Fluorescence, Transmission Assay, Electron Microscopy

Journal: Cell Death & Disease

Article Title: High glucose levels promote glycolysis and cholesterol synthesis via ERRα and suppress the autophagy–lysosomal pathway in endometrial cancer

doi: 10.1038/s41419-025-07499-y

Figure Lengend Snippet: A H&E staining of tumor sections from EC patients without and with DM. B Representative immunohistochemical images of ERRα in EC patients without and with DM. C IRS of ERRα was calculated and compared between the patients with and without DM. D The cellular morphology of organoids at different magnifications was evaluated using a light microscope and H&E staining. E After different treatments, the IC 50 values in the corresponding groups of organoids were determined using a CCK8 assay. F – H The morphological changes in EC organoids at 0 h, 48 h, and 96 h were evaluated using light microscopy after treatment with XCT790 (12.5 µM) alone as well as in combination with a carboplatin concentration gradient, metformin (0.35 mM) alone as well as in combination with a carboplatin concentration gradient and simvastatin (20 µM) alone as well as in combination with a carboplatin concentration gradient, respectively. I – K The cell viability treated with different treatments was evaluated by CCK8 with three replications. The data are shown as the means ± standard deviations. Statistical test: paired t test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. EC endometrial cancer, H&E hematoxylin and eosin, IRS Immunoreactivity score.

Article Snippet: HEC-1A and KLE cells were incubated with 5 µM and 10 µM simvastatin (indole dimethyl sulfoxide [DMSO]) (#Y190601, MP Biomedicals LLC, CA, USA) for 24 h, they were treated with 10 mM 2-deoxy-D-glucose (2-DG) (#HY-13966, MedChemExpress LLC, NJ, USA) for 24 h, or with 10 μM 3-bromopyruvic acid (3-BrPA) (#HY-19992, MedChemExpress LLC, NJ, USA) for 24 h.

Techniques: Staining, Immunohistochemical staining, Light Microscopy, CCK-8 Assay, Concentration Assay