Journal: American Journal of Cancer Research
Article Title: Predictive value of ERCC1, ERCC2, ERCC4, and glutathione S-Transferase Pi expression for the efficacy and safety of FOLFIRINOX in patients with unresectable pancreatic cancer
doi:
Figure Lengend Snippet: Immunohistochemical staining of patient specimens. A-D. Representative images of immunohistochemical staining of the four proteins to illustrate typical intensity and percentage staining scores (both on a scale of 1-3). A. Excision repair cross-complementing (ERCC): intensity score 1, percentage score 3. B. ERCC2: intensity score 3, percentage score 2. C. ERCC4: intensity score 3, percentage score 2. D. Glutathione S-transferase Pi, intensity score 3, percentage score 3. Scale bar = 500 μm.
Article Snippet: Sections were then incubated with one of the following primary antibodies for 60 min at room temperature: anti-ERCC1 (#12345, Cell Signaling Technology, Danvers, MA, USA, 1:100 dilution), anti-ERCC2 (10818-1-AP, Proteintech, Rosemont, IL, USA, 1:75), anti-ERCC4 (IHC-00263, Bethyl Laboratories, Montgomery, TX, USA, 1:300), and anti-GSTP1 (#3369, Cell Signaling Technology, 1:800).
Techniques: Immunohistochemical staining, Staining