echo 555 acoustic liquid handler  (Labcyte)


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    Name:
    Echo 555 Liquid Handler
    Description:
    The Echo 555 Liquid Handler produce the high quality data with the highest throughput rate significantly reducing costs when using for sample management or high throughput plate preparation Our built in DMSO hydration measurements ensure accurate transfer and allow users to track compound library integrity
    Catalog Number:
    001-5234
    Price:
    None
    Category:
    Liquid handler
    Quantity:
    1
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    Structured Review

    Labcyte echo 555 acoustic liquid handler
    LOPAC 1280  library compound dispensing layout in a 1536-well assay plate. Compounds were dispensed using the Echo 555.
    The Echo 555 Liquid Handler produce the high quality data with the highest throughput rate significantly reducing costs when using for sample management or high throughput plate preparation Our built in DMSO hydration measurements ensure accurate transfer and allow users to track compound library integrity
    https://www.bioz.com/result/echo 555 acoustic liquid handler/product/Labcyte
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    echo 555 acoustic liquid handler - by Bioz Stars, 2020-02
    95/100 stars

    Images

    1) Product Images from "Miniaturized GPCR Signaling Studies in 1536-Well Format"

    Article Title: Miniaturized GPCR Signaling Studies in 1536-Well Format

    Journal: Journal of Biomolecular Techniques : JBT

    doi:

    LOPAC 1280  library compound dispensing layout in a 1536-well assay plate. Compounds were dispensed using the Echo 555.
    Figure Legend Snippet: LOPAC 1280 library compound dispensing layout in a 1536-well assay plate. Compounds were dispensed using the Echo 555.

    Techniques Used:

    Secondary screening in a 1536-well assay plate layout for 12 hit compounds. Compounds were titrated using the Echo 555.
    Figure Legend Snippet: Secondary screening in a 1536-well assay plate layout for 12 hit compounds. Compounds were titrated using the Echo 555.

    Techniques Used:

    A: Labcyte Echo 555 acoustic liquid handler. B: Schematic diagram of the acoustic drop ejection process. c: Time lapsed photographs of the 2.5-nL droplet ejection.
    Figure Legend Snippet: A: Labcyte Echo 555 acoustic liquid handler. B: Schematic diagram of the acoustic drop ejection process. c: Time lapsed photographs of the 2.5-nL droplet ejection.

    Techniques Used:

    Related Articles

    Functional Assay:

    Article Title: Direct Activation of β-Cell KATP
    Article Snippet: The plates were transferred to a Hamamatsu Functional Drug Screening System 6000 (FDSS6000; Hamamatsu, Tokyo, Japan) and 20 μ l/well of test compounds in assay buffer (as prepared below) was added. .. Test compounds from the library of Vanderbilt Institute of Chemical Biology were dispensed into in polypropylene 384-well plates (Greiner Bio-One, Monroe, NC) using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) diluted in assay buffer to 2× final concentrations to generate 4- or 11-point 3-fold serial dilution series.

    Article Title: ML418: The first selective, sub-micromolar pore blocker of Kir7.1 potassium channels
    Article Snippet: Test compounds from the VICB Library were dispensed into 384-well plates using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) and diluted to a 2X concentration. .. Cell plates were transferred to a Hamamatsu Functional Drug Screening System 6000 (FDSS6000; Hamamatsu, Tokyo, Japan) where 20 µL/well test compounds were dispensed into wells to a nominal concentration of 10 µM.

    Article Title: Discovery and Characterization of a Potent and Selective Inhibitor of Aedes aegypti Inward Rectifier Potassium Channels
    Article Snippet: The plates were transferred to a Hamamatsu Functional Drug Screening System 6000 (FDSS6000; Hamamatsu, Hamamatsu (or Bridgewater, NJ), Japan) where 20 µL/well of test compounds in assay buffer (as prepared below) were added and allowed to incubate with the cells for 20 min. After the incubation period, a baseline recording was collected at 1 Hz for 10 s (excitation 470±20 nm, emission 540±30 nm) followed by a Tl+ stimulus buffer addition (10 µL/well) and data collection for an additional 4 min. .. The test compounds were transferred to daughter polypropylene 384-well plates (Greiner Bio-One, Monroe, NC) using an Echo555 liquid handler (Labcyte, Sunnyvale, CA), and then diluted into assay buffer to generate a 2X stock in 0.6% DMSO (0.3% final).

    Luciferase:

    Article Title: POSTER SESSION ABSTRACTS
    Article Snippet: The assay is based on the principle that cAMP stimulates protein kinase A holoenzyme activity, decreasing available ATP and leading to decreased light production in a luciferase reaction. .. Together with the Labcyte Echo 555 acoustic liquid handler and the Deerac Fluidics Equator HTS reagent dispenser, compounds can be screened in 1536-well format for effects on GPCRs.

    Reporter Assay:

    Article Title: ML418: The first selective, sub-micromolar pore blocker of Kir7.1 potassium channels
    Article Snippet: HTS for Kir7.1-M125R modulators was performed using a Tl+ -flux reporter assay essentially as described previously , , . .. Test compounds from the VICB Library were dispensed into 384-well plates using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) and diluted to a 2X concentration.

    Positive Control:

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: For the second screen using azetidine-core libraries the compounds were pre-loaded in empty 384-well plates by sonication using an Echo 555 Liquid Handler (Labcyte) and the enzyme-probe mixture was added to the plate (50 μL/well, 0.5 μM IDE, 30 nM FL-6b, 4 ) the Multidrop Combi-nL Reagent Dispenser (Thermo Scientific). .. The final compound concentrations were 20 μM in both screens, and IDE inhibitor 6bK ( 1 ) was used as a positive control at 1 μM final concentration.

    Article Title: Sensitive ADAR editing reporter in cancer cells enables high-throughput screening of small molecule libraries
    Article Snippet: For screening purpose, the compound solutions (10 mM in DMSO) were transferred from Labcyte 384 LDVplates (LP-0200) to 384 polypropylene plates (Greiner # 781201) using an Echo 555 acoustic liquid handler (LabCyte). .. The compound solutions (60 nl) were dispensed into columns 1–22 and an equivalent volume of DMSO for the negative control (0% inhibition), and a 10 mM DMSO solution containing Nluc Inhibitor 1 (100% inhibition) for the positive control in columns 23 and 24, respectively.

    Cytometry:

    Article Title: High-throughput flow cytometry (HTFC) drug combination discovery with novel synergy analysis software, SynScreen
    Article Snippet: The drugs were added with the Echo® 555 Liquid Handler (Labcyte, San Jose, CA) into 384-well plates (Greiner Bio-One 784201, Monroe, NC) to a final DMSO concentration of 1%. .. HTFC data were collected with a HyperCyt® platform (IntelliCyt) configured to an Accuri C6 Plus flow cytometer (BD Biosciences, Franklin Lakes, NJ).

    Article Title: High throughput flow cytometry identifies small molecule inhibitors for drug repurposing in T-ALL
    Article Snippet: Paragraph title: High Throughput Flow Cytometry Viability Testing ... An Echo® 555 Liquid Handler (Labcyte, San Jose, CA) was used both to create intermediate source plates and to add inhibitors to assay plates.

    Incubation:

    Article Title: Direct Activation of β-Cell KATP
    Article Snippet: After a 20-minute incubation period, a baseline recording was collected at 1 Hz for 10 seconds (excitation 470 ± 20 nm, emission 540 ± 30 nm) followed by addition of the Tl+ stimulus buffer (10 μ l/well) and data collection for an additional 4 minutes. .. Test compounds from the library of Vanderbilt Institute of Chemical Biology were dispensed into in polypropylene 384-well plates (Greiner Bio-One, Monroe, NC) using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) diluted in assay buffer to 2× final concentrations to generate 4- or 11-point 3-fold serial dilution series.

    Article Title: ML418: The first selective, sub-micromolar pore blocker of Kir7.1 potassium channels
    Article Snippet: The following day, the cells were incubated with dye-loading assay buffer (Hank’s Balanced Salt Solution, 20 mM HEPES, pH 7.3) containing 0.01% (w/v) Pluronic F-127 (Life Technologies) and 1.2 µM Thallos-AM (TEFlabs, Austin, TX) Tl+ reporter dye. .. Test compounds from the VICB Library were dispensed into 384-well plates using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) and diluted to a 2X concentration.

    Article Title: High-throughput flow cytometry (HTFC) drug combination discovery with novel synergy analysis software, SynScreen
    Article Snippet: These cell lines were cultured in RPMI-1640 medium supplemented with 2 mM L-glutamine, 100 U/mL penicillin-streptomycin and 10% heat-inactivated fetal bovine serum and incubated in a humidified atmosphere with 5% CO2 at 37°C. .. The drugs were added with the Echo® 555 Liquid Handler (Labcyte, San Jose, CA) into 384-well plates (Greiner Bio-One 784201, Monroe, NC) to a final DMSO concentration of 1%.

    Article Title: Discovery and Characterization of a Potent and Selective Inhibitor of Aedes aegypti Inward Rectifier Potassium Channels
    Article Snippet: The plates were transferred to a Hamamatsu Functional Drug Screening System 6000 (FDSS6000; Hamamatsu, Hamamatsu (or Bridgewater, NJ), Japan) where 20 µL/well of test compounds in assay buffer (as prepared below) were added and allowed to incubate with the cells for 20 min. After the incubation period, a baseline recording was collected at 1 Hz for 10 s (excitation 470±20 nm, emission 540±30 nm) followed by a Tl+ stimulus buffer addition (10 µL/well) and data collection for an additional 4 min. .. The test compounds were transferred to daughter polypropylene 384-well plates (Greiner Bio-One, Monroe, NC) using an Echo555 liquid handler (Labcyte, Sunnyvale, CA), and then diluted into assay buffer to generate a 2X stock in 0.6% DMSO (0.3% final).

    Article Title: Discovery of Small-Molecule Inhibitors Targeting the E3 Ubiquitin Ligase Activity of the Herpes Simplex Virus 1 ICP0 Protein Using an In Vitro High-Throughput Screening Assay
    Article Snippet: The compounds (15 μM) were transferred acoustically to low-volume, 384-well plates (Corning) using an Echo 555 liquid handler (Labcyte Inc.). .. After the ubiquitination reaction at 30°C for 1 h, detection reagents containing biotin-TUBE, streptavidin-terbium, and anti-GST-d2 monoclonal antibody were added to the plates and incubated for 1 h at room temperature.

    Article Title: Identification of Specific and Nonspecific Inhibitors of Bacillus anthracis Type III Pantothenate Kinase (PanK)
    Article Snippet: Following a 30-min incubation period the increase in fluorescence 530/590 was read on an EnVision Multilabel Reader (PerkinElmer). .. For IC50 determinations of the test compounds,[ ] plates were predrugged using a Labcyte ECHO555 Liquid Handler.

    Activity Assay:

    Article Title: Miniaturized GPCR Signaling Studies in 1536-Well Format
    Article Snippet: G protein-coupled receptors (GPCRs) are involved in various physiological processes, such as behavior changes, mood alteration, and regulation of immune-system activity. .. Together with the Labcyte Echo 555 acoustic liquid handler and the Deerac Fluidics Equator HTS reagent dispenser, this setup can screen compounds in 96-, 384-, and 1536-well formats for their effects on GPCRs.

    Article Title: POSTER SESSION ABSTRACTS
    Article Snippet: The assay is based on the principle that cAMP stimulates protein kinase A holoenzyme activity, decreasing available ATP and leading to decreased light production in a luciferase reaction. .. Together with the Labcyte Echo 555 acoustic liquid handler and the Deerac Fluidics Equator HTS reagent dispenser, compounds can be screened in 1536-well format for effects on GPCRs.

    Apoptosis Assay:

    Article Title: High-throughput flow cytometry (HTFC) drug combination discovery with novel synergy analysis software, SynScreen
    Article Snippet: The apoptosis assay consisted of drug pairs tested in 9-point 1:1 and 1:2 fixed ratio dose responses. .. The drugs were added with the Echo® 555 Liquid Handler (Labcyte, San Jose, CA) into 384-well plates (Greiner Bio-One 784201, Monroe, NC) to a final DMSO concentration of 1%.

    Expressing:

    Article Title: A screening strategy for the discovery of drugs that reduce C/EBPβ-LIP translation with potential calorie restriction mimetic properties
    Article Snippet: Library screening HEK293T cells stably expressing FireflyRe-ini /RenillaIni vector were seeded at a density of 1.5 × 104 in 30 μl media per well of a 384-well plate (Greiner CELLSTAR® 384 well plates, white) and grown overnight. .. Next day, the cells were treated for eight hours with 780 FDA approved drugs library (#BML-2843-0100, Enzo Lifescience) (final concentration 10 μM), 0.5% v/v DMSO and rapamycin (final concentration 200 nM) using an Echo® 555 Liquid Handler (Labcyte) transferring up to 150 nl to each well.

    Article Title: ML418: The first selective, sub-micromolar pore blocker of Kir7.1 potassium channels
    Article Snippet: T-REx-HEK-293-Kir7.1-M125R cells (20,000/well) were plated in clear-bottomed, black-walled, 384-well plates, and cultured overnight in Delbecco’s Modified Eagle’s Medium (DMEM) containing 10% dialyzed FBS and 1 µg/ml tetracycline to induce Kir7.1-M125R expression. .. Test compounds from the VICB Library were dispensed into 384-well plates using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) and diluted to a 2X concentration.

    Article Title: Discovery and Characterization of a Potent and Selective Inhibitor of Aedes aegypti Inward Rectifier Potassium Channels
    Article Snippet: Also, Tl+ flux assays on Kir3.1/3.2/mGlu8 expressing cell, required addition of an EC80 concentration of glutamate (Sigma-Aldrich, St. Louis, MO) with the Tl+ stimulus buffer . .. The test compounds were transferred to daughter polypropylene 384-well plates (Greiner Bio-One, Monroe, NC) using an Echo555 liquid handler (Labcyte, Sunnyvale, CA), and then diluted into assay buffer to generate a 2X stock in 0.6% DMSO (0.3% final).

    Modification:

    Article Title: ML418: The first selective, sub-micromolar pore blocker of Kir7.1 potassium channels
    Article Snippet: T-REx-HEK-293-Kir7.1-M125R cells (20,000/well) were plated in clear-bottomed, black-walled, 384-well plates, and cultured overnight in Delbecco’s Modified Eagle’s Medium (DMEM) containing 10% dialyzed FBS and 1 µg/ml tetracycline to induce Kir7.1-M125R expression. .. Test compounds from the VICB Library were dispensed into 384-well plates using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) and diluted to a 2X concentration.

    Kinase Assay:

    Article Title: Identification of Specific and Nonspecific Inhibitors of Bacillus anthracis Type III Pantothenate Kinase (PanK)
    Article Snippet: Paragraph title: High-throughput pantothenate kinase assay: ... For IC50 determinations of the test compounds,[ ] plates were predrugged using a Labcyte ECHO555 Liquid Handler.

    Over Expression:

    Article Title: RNA interference screen identifies NAA10 as a regulator of PXR transcription
    Article Snippet: AsPC-1 cells with PXR knockdown or overexpression were induced with 1 μg/mL dox (for the induced groups only) and plated at 2000 cells/well in 40 μL of RPMI media containing 10% fetal bovine serum. .. After 24 h, the cells were treated with either DMSO or respective compounds by using an Echo 555 Liquid Handler (Labcyte), and the plates were returned to incubate.

    Electroporation:

    Article Title: POSTER SESSION ABSTRACTS
    Article Snippet: Together with the Labcyte Echo 555 acoustic liquid handler and the Deerac Fluidics Equator HTS reagent dispenser, compounds can be screened in 1536-well format for effects on GPCRs. .. Electroporation is a valuable tool for nucleic acid delivery because it can be used for a wide variety of cell types.

    Flow Cytometry:

    Article Title: High-throughput flow cytometry (HTFC) drug combination discovery with novel synergy analysis software, SynScreen
    Article Snippet: The drugs were added with the Echo® 555 Liquid Handler (Labcyte, San Jose, CA) into 384-well plates (Greiner Bio-One 784201, Monroe, NC) to a final DMSO concentration of 1%. .. HTFC data were collected with a HyperCyt® platform (IntelliCyt) configured to an Accuri C6 Plus flow cytometer (BD Biosciences, Franklin Lakes, NJ).

    Article Title: High throughput flow cytometry identifies small molecule inhibitors for drug repurposing in T-ALL
    Article Snippet: Paragraph title: High Throughput Flow Cytometry Viability Testing ... An Echo® 555 Liquid Handler (Labcyte, San Jose, CA) was used both to create intermediate source plates and to add inhibitors to assay plates.

    Stable Transfection:

    Article Title: A screening strategy for the discovery of drugs that reduce C/EBPβ-LIP translation with potential calorie restriction mimetic properties
    Article Snippet: Library screening HEK293T cells stably expressing FireflyRe-ini /RenillaIni vector were seeded at a density of 1.5 × 104 in 30 μl media per well of a 384-well plate (Greiner CELLSTAR® 384 well plates, white) and grown overnight. .. Next day, the cells were treated for eight hours with 780 FDA approved drugs library (#BML-2843-0100, Enzo Lifescience) (final concentration 10 μM), 0.5% v/v DMSO and rapamycin (final concentration 200 nM) using an Echo® 555 Liquid Handler (Labcyte) transferring up to 150 nl to each well.

    Concentration Assay:

    Article Title: RNA interference screen identifies NAA10 as a regulator of PXR transcription
    Article Snippet: After 24 h, the cells were treated with either DMSO or respective compounds by using an Echo 555 Liquid Handler (Labcyte), and the plates were returned to incubate. .. Appropriate volumes of DMSO were added to achieve a final concentration across all plates of 0.4%.

    Article Title: A screening strategy for the discovery of drugs that reduce C/EBPβ-LIP translation with potential calorie restriction mimetic properties
    Article Snippet: .. Next day, the cells were treated for eight hours with 780 FDA approved drugs library (#BML-2843-0100, Enzo Lifescience) (final concentration 10 μM), 0.5% v/v DMSO and rapamycin (final concentration 200 nM) using an Echo® 555 Liquid Handler (Labcyte) transferring up to 150 nl to each well. ..

    Article Title: Identification of novel small molecules that inhibit STAT3-dependent transcription and function
    Article Snippet: JAK inhibitor Pyr6 was purchased from Merck Millipore (Germany) and was used in the concentration 1 μM. .. Compound dispensing for the screening was performed using ECHO 555 acoustic liquid handler (Labcyte Inc., the USA).

    Article Title: ML418: The first selective, sub-micromolar pore blocker of Kir7.1 potassium channels
    Article Snippet: .. Test compounds from the VICB Library were dispensed into 384-well plates using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) and diluted to a 2X concentration. .. Cell plates were transferred to a Hamamatsu Functional Drug Screening System 6000 (FDSS6000; Hamamatsu, Tokyo, Japan) where 20 µL/well test compounds were dispensed into wells to a nominal concentration of 10 µM.

    Article Title: High-throughput flow cytometry (HTFC) drug combination discovery with novel synergy analysis software, SynScreen
    Article Snippet: .. The drugs were added with the Echo® 555 Liquid Handler (Labcyte, San Jose, CA) into 384-well plates (Greiner Bio-One 784201, Monroe, NC) to a final DMSO concentration of 1%. .. To prevent excess evaporation and to optimize conditions, the assay plates were flushed with ambient air and sealed with a PlateLoc Thermal Microplate Sealer (Agilent, Santa Clara, CA) prior to incubation.

    Article Title: High-Throughput Screening of Myometrial Calcium-Mobilization to Identify Modulators of Uterine Contractility
    Article Snippet: Test compounds in 40 μL of wash buffer were prepared by transferring 80 nL into a 384-well polypropylene plate using the ECHO 555 acoustic liquid handler (Labcyte, Sunnyvale, CA) from a 10mM stock. .. Test compounds (10μM final concentration) were added during the compound addition, with OT (EC80 ) added during the OT addition.

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: For the second screen using azetidine-core libraries the compounds were pre-loaded in empty 384-well plates by sonication using an Echo 555 Liquid Handler (Labcyte) and the enzyme-probe mixture was added to the plate (50 μL/well, 0.5 μM IDE, 30 nM FL-6b, 4 ) the Multidrop Combi-nL Reagent Dispenser (Thermo Scientific). .. The final compound concentrations were 20 μM in both screens, and IDE inhibitor 6bK ( 1 ) was used as a positive control at 1 μM final concentration.

    Article Title: Discovery and Characterization of a Potent and Selective Inhibitor of Aedes aegypti Inward Rectifier Potassium Channels
    Article Snippet: Also, Tl+ flux assays on Kir3.1/3.2/mGlu8 expressing cell, required addition of an EC80 concentration of glutamate (Sigma-Aldrich, St. Louis, MO) with the Tl+ stimulus buffer . .. The test compounds were transferred to daughter polypropylene 384-well plates (Greiner Bio-One, Monroe, NC) using an Echo555 liquid handler (Labcyte, Sunnyvale, CA), and then diluted into assay buffer to generate a 2X stock in 0.6% DMSO (0.3% final).

    Article Title: High throughput flow cytometry identifies small molecule inhibitors for drug repurposing in T-ALL
    Article Snippet: An Echo® 555 Liquid Handler (Labcyte, San Jose, CA) was used both to create intermediate source plates and to add inhibitors to assay plates. .. The thirty-one drugs were added to the plates to generate 10-point dose responses, with assay final DMSO concentration at < 1%.

    Library Screening:

    Article Title: A screening strategy for the discovery of drugs that reduce C/EBPβ-LIP translation with potential calorie restriction mimetic properties
    Article Snippet: Paragraph title: Library screening ... Next day, the cells were treated for eight hours with 780 FDA approved drugs library (#BML-2843-0100, Enzo Lifescience) (final concentration 10 μM), 0.5% v/v DMSO and rapamycin (final concentration 200 nM) using an Echo® 555 Liquid Handler (Labcyte) transferring up to 150 nl to each well.

    Serial Dilution:

    Article Title: Direct Activation of β-Cell KATP
    Article Snippet: .. Test compounds from the library of Vanderbilt Institute of Chemical Biology were dispensed into in polypropylene 384-well plates (Greiner Bio-One, Monroe, NC) using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) diluted in assay buffer to 2× final concentrations to generate 4- or 11-point 3-fold serial dilution series. ..

    Transferring:

    Article Title: A screening strategy for the discovery of drugs that reduce C/EBPβ-LIP translation with potential calorie restriction mimetic properties
    Article Snippet: .. Next day, the cells were treated for eight hours with 780 FDA approved drugs library (#BML-2843-0100, Enzo Lifescience) (final concentration 10 μM), 0.5% v/v DMSO and rapamycin (final concentration 200 nM) using an Echo® 555 Liquid Handler (Labcyte) transferring up to 150 nl to each well. ..

    Article Title: High-Throughput Screening of Myometrial Calcium-Mobilization to Identify Modulators of Uterine Contractility
    Article Snippet: .. Test compounds in 40 μL of wash buffer were prepared by transferring 80 nL into a 384-well polypropylene plate using the ECHO 555 acoustic liquid handler (Labcyte, Sunnyvale, CA) from a 10mM stock. .. Test compounds (10μM final concentration) were added during the compound addition, with OT (EC80 ) added during the OT addition.

    Cell Culture:

    Article Title: ML418: The first selective, sub-micromolar pore blocker of Kir7.1 potassium channels
    Article Snippet: T-REx-HEK-293-Kir7.1-M125R cells (20,000/well) were plated in clear-bottomed, black-walled, 384-well plates, and cultured overnight in Delbecco’s Modified Eagle’s Medium (DMEM) containing 10% dialyzed FBS and 1 µg/ml tetracycline to induce Kir7.1-M125R expression. .. Test compounds from the VICB Library were dispensed into 384-well plates using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) and diluted to a 2X concentration.

    Article Title: High-throughput flow cytometry (HTFC) drug combination discovery with novel synergy analysis software, SynScreen
    Article Snippet: These cell lines were cultured in RPMI-1640 medium supplemented with 2 mM L-glutamine, 100 U/mL penicillin-streptomycin and 10% heat-inactivated fetal bovine serum and incubated in a humidified atmosphere with 5% CO2 at 37°C. .. The drugs were added with the Echo® 555 Liquid Handler (Labcyte, San Jose, CA) into 384-well plates (Greiner Bio-One 784201, Monroe, NC) to a final DMSO concentration of 1%.

    Generated:

    Article Title: Discovery and Characterization of a Potent and Selective Inhibitor of Aedes aegypti Inward Rectifier Potassium Channels
    Article Snippet: The test compounds were transferred to daughter polypropylene 384-well plates (Greiner Bio-One, Monroe, NC) using an Echo555 liquid handler (Labcyte, Sunnyvale, CA), and then diluted into assay buffer to generate a 2X stock in 0.6% DMSO (0.3% final). .. Concentration-response curves (CRCs) were generated by screening compounds at 3-fold dilution series in 4-point (1 µM–30 µM) or 11-point (1 nM–30 µM) CRCs.

    Inhibition:

    Article Title: Sensitive ADAR editing reporter in cancer cells enables high-throughput screening of small molecule libraries
    Article Snippet: For screening purpose, the compound solutions (10 mM in DMSO) were transferred from Labcyte 384 LDVplates (LP-0200) to 384 polypropylene plates (Greiner # 781201) using an Echo 555 acoustic liquid handler (LabCyte). .. The compound solutions (60 nl) were dispensed into columns 1–22 and an equivalent volume of DMSO for the negative control (0% inhibition), and a 10 mM DMSO solution containing Nluc Inhibitor 1 (100% inhibition) for the positive control in columns 23 and 24, respectively.

    Sonication:

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: .. For the second screen using azetidine-core libraries the compounds were pre-loaded in empty 384-well plates by sonication using an Echo 555 Liquid Handler (Labcyte) and the enzyme-probe mixture was added to the plate (50 μL/well, 0.5 μM IDE, 30 nM FL-6b, 4 ) the Multidrop Combi-nL Reagent Dispenser (Thermo Scientific). .. The final compound concentrations were 20 μM in both screens, and IDE inhibitor 6bK ( 1 ) was used as a positive control at 1 μM final concentration.

    Screening Assay:

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: Paragraph title: Fluorescence anisotropy high-throughput screening assay ... For the second screen using azetidine-core libraries the compounds were pre-loaded in empty 384-well plates by sonication using an Echo 555 Liquid Handler (Labcyte) and the enzyme-probe mixture was added to the plate (50 μL/well, 0.5 μM IDE, 30 nM FL-6b, 4 ) the Multidrop Combi-nL Reagent Dispenser (Thermo Scientific).

    Fluorescence:

    Article Title: ML418: The first selective, sub-micromolar pore blocker of Kir7.1 potassium channels
    Article Snippet: Test compounds from the VICB Library were dispensed into 384-well plates using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) and diluted to a 2X concentration. .. After a 20-min incubation period, baseline fluorescence (excitation 470 ± 20 nm, emission 540 ± 30 nm) was recorded at 1Hz for 10 sec before addition of thallium stimulus buffer containing (in mM): 125 NaHCO3 , 1.8 CaSO4 , 1 MgSO4 , 5 glucose, and 1.8 Tl2 SO4.

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: Paragraph title: Fluorescence anisotropy high-throughput screening assay ... For the second screen using azetidine-core libraries the compounds were pre-loaded in empty 384-well plates by sonication using an Echo 555 Liquid Handler (Labcyte) and the enzyme-probe mixture was added to the plate (50 μL/well, 0.5 μM IDE, 30 nM FL-6b, 4 ) the Multidrop Combi-nL Reagent Dispenser (Thermo Scientific).

    Article Title: Identification of Specific and Nonspecific Inhibitors of Bacillus anthracis Type III Pantothenate Kinase (PanK)
    Article Snippet: Following a 30-min incubation period the increase in fluorescence 530/590 was read on an EnVision Multilabel Reader (PerkinElmer). .. For IC50 determinations of the test compounds,[ ] plates were predrugged using a Labcyte ECHO555 Liquid Handler.

    Mutagenesis:

    Article Title: ML418: The first selective, sub-micromolar pore blocker of Kir7.1 potassium channels
    Article Snippet: The Kir7.1-M125R mutation increases the channel unitary conductance and enables robust Tl+ flux measurement that cannot be achieved with the WT channel . .. Test compounds from the VICB Library were dispensed into 384-well plates using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) and diluted to a 2X concentration.

    Size-exclusion Chromatography:

    Article Title: ML418: The first selective, sub-micromolar pore blocker of Kir7.1 potassium channels
    Article Snippet: Test compounds from the VICB Library were dispensed into 384-well plates using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) and diluted to a 2X concentration. .. After a 20-min incubation period, baseline fluorescence (excitation 470 ± 20 nm, emission 540 ± 30 nm) was recorded at 1Hz for 10 sec before addition of thallium stimulus buffer containing (in mM): 125 NaHCO3 , 1.8 CaSO4 , 1 MgSO4 , 5 glucose, and 1.8 Tl2 SO4.

    Viability Assay:

    Article Title: High-throughput flow cytometry (HTFC) drug combination discovery with novel synergy analysis software, SynScreen
    Article Snippet: The drugs were added with the Echo® 555 Liquid Handler (Labcyte, San Jose, CA) into 384-well plates (Greiner Bio-One 784201, Monroe, NC) to a final DMSO concentration of 1%. .. The cytotoxicity plates were incubated for 48 h, and the apoptosis plates were incubated 2–24 h. The propidium iodide (PI) cell viability assay was performed as previously described , and the MultiCyt Apoptosis Kit (IntelliCyt, Albuquerque, NM) was used according to the manufacturer’s protocol.

    Plasmid Preparation:

    Article Title: A screening strategy for the discovery of drugs that reduce C/EBPβ-LIP translation with potential calorie restriction mimetic properties
    Article Snippet: Library screening HEK293T cells stably expressing FireflyRe-ini /RenillaIni vector were seeded at a density of 1.5 × 104 in 30 μl media per well of a 384-well plate (Greiner CELLSTAR® 384 well plates, white) and grown overnight. .. Next day, the cells were treated for eight hours with 780 FDA approved drugs library (#BML-2843-0100, Enzo Lifescience) (final concentration 10 μM), 0.5% v/v DMSO and rapamycin (final concentration 200 nM) using an Echo® 555 Liquid Handler (Labcyte) transferring up to 150 nl to each well.

    Software:

    Article Title: Direct Activation of β-Cell KATP
    Article Snippet: Test compounds from the library of Vanderbilt Institute of Chemical Biology were dispensed into in polypropylene 384-well plates (Greiner Bio-One, Monroe, NC) using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) diluted in assay buffer to 2× final concentrations to generate 4- or 11-point 3-fold serial dilution series. .. Tl+ flux data were analyzed as previously described ( ) using a combination of Excel (Microsoft Corp, Redmond, WA) with XLfit add-in (IDBS, Guildford, Surrey, UK) and OriginPro (OriginLab, Northampton, MA) software.

    Article Title: Discovery and Characterization of a Potent and Selective Inhibitor of Aedes aegypti Inward Rectifier Potassium Channels
    Article Snippet: The test compounds were transferred to daughter polypropylene 384-well plates (Greiner Bio-One, Monroe, NC) using an Echo555 liquid handler (Labcyte, Sunnyvale, CA), and then diluted into assay buffer to generate a 2X stock in 0.6% DMSO (0.3% final). .. Tl+ flux data were analyzed as previously described , , using a combination of Excel (Microsoft Corp, Redmond, WA) with XLfit add-in (IDBS, Guildford, Surrey, UK) and OriginPro (OriginLab, Northampton, MA) software.

    Negative Control:

    Article Title: Sensitive ADAR editing reporter in cancer cells enables high-throughput screening of small molecule libraries
    Article Snippet: For screening purpose, the compound solutions (10 mM in DMSO) were transferred from Labcyte 384 LDVplates (LP-0200) to 384 polypropylene plates (Greiner # 781201) using an Echo 555 acoustic liquid handler (LabCyte). .. The compound solutions (60 nl) were dispensed into columns 1–22 and an equivalent volume of DMSO for the negative control (0% inhibition), and a 10 mM DMSO solution containing Nluc Inhibitor 1 (100% inhibition) for the positive control in columns 23 and 24, respectively.

    Flux Assay:

    Article Title: Direct Activation of β-Cell KATP
    Article Snippet: For the Tl+ flux assay on Kir 3.1/3.2-expressing cells, the thallium stimulus buffer contains 12 mM Tl2 SO4 and either an EC20 or EC80 of glutamate (Sigma-Aldrich, St. Louis, MO). .. Test compounds from the library of Vanderbilt Institute of Chemical Biology were dispensed into in polypropylene 384-well plates (Greiner Bio-One, Monroe, NC) using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) diluted in assay buffer to 2× final concentrations to generate 4- or 11-point 3-fold serial dilution series.

    Spectrophotometry:

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: The optimum signal was obtained using 0.5 μM IDE ( ) and 5 to 30 nM of probe FL-6b ( 4 ) depending on the spectrophotometer used (excitation 492 nm, emission 523 nm). .. For the second screen using azetidine-core libraries the compounds were pre-loaded in empty 384-well plates by sonication using an Echo 555 Liquid Handler (Labcyte) and the enzyme-probe mixture was added to the plate (50 μL/well, 0.5 μM IDE, 30 nM FL-6b, 4 ) the Multidrop Combi-nL Reagent Dispenser (Thermo Scientific).

    Evaporation:

    Article Title: High-throughput flow cytometry (HTFC) drug combination discovery with novel synergy analysis software, SynScreen
    Article Snippet: The drugs were added with the Echo® 555 Liquid Handler (Labcyte, San Jose, CA) into 384-well plates (Greiner Bio-One 784201, Monroe, NC) to a final DMSO concentration of 1%. .. To prevent excess evaporation and to optimize conditions, the assay plates were flushed with ambient air and sealed with a PlateLoc Thermal Microplate Sealer (Agilent, Santa Clara, CA) prior to incubation.

    Produced:

    Article Title: Identification of novel small molecules that inhibit STAT3-dependent transcription and function
    Article Snippet: The compound library, ENAMINE Drug-Like Diversity Set used for the screening, was a property of Karolinska High Throughput Center and contained 28,000 individual compounds dissolved in DMSO (produced by ENAMINE Ltd., Ukraine). .. Compound dispensing for the screening was performed using ECHO 555 acoustic liquid handler (Labcyte Inc., the USA).

    Activation Assay:

    Article Title: Discovery and Characterization of a Potent and Selective Inhibitor of Aedes aegypti Inward Rectifier Potassium Channels
    Article Snippet: The test compounds were transferred to daughter polypropylene 384-well plates (Greiner Bio-One, Monroe, NC) using an Echo555 liquid handler (Labcyte, Sunnyvale, CA), and then diluted into assay buffer to generate a 2X stock in 0.6% DMSO (0.3% final). .. For Tl+ flux assays on Kir6.2/SUR1 expressing cells, test compounds were diluted in assay buffer containing diazoxide (250 µM final) to induce channel activation .

    High Throughput Screening Assay:

    Article Title: Identification of novel small molecules that inhibit STAT3-dependent transcription and function
    Article Snippet: The compound library, ENAMINE Drug-Like Diversity Set used for the screening, was a property of Karolinska High Throughput Center and contained 28,000 individual compounds dissolved in DMSO (produced by ENAMINE Ltd., Ukraine). .. Compound dispensing for the screening was performed using ECHO 555 acoustic liquid handler (Labcyte Inc., the USA).

    Article Title: Miniaturized GPCR Signaling Studies in 1536-Well Format
    Article Snippet: Together with the Labcyte Echo 555 acoustic liquid handler and the Deerac Fluidics Equator HTS reagent dispenser, this setup can screen compounds in 96-, 384-, and 1536-well formats for their effects on GPCRs. .. Implementing a high-throughput miniaturized GPCR assay as demonstrated here allows effective screening for potential drug candidates.

    Article Title: ML418: The first selective, sub-micromolar pore blocker of Kir7.1 potassium channels
    Article Snippet: Paragraph title: High-throughput screening (HTS) ... Test compounds from the VICB Library were dispensed into 384-well plates using an Echo555 liquid handler (Labcyte, Sunnyvale, CA) and diluted to a 2X concentration.

    Article Title: Substrate-selective inhibitors that reprogram the activity of insulin-degrading enzyme
    Article Snippet: Paragraph title: Fluorescence anisotropy high-throughput screening assay ... For the second screen using azetidine-core libraries the compounds were pre-loaded in empty 384-well plates by sonication using an Echo 555 Liquid Handler (Labcyte) and the enzyme-probe mixture was added to the plate (50 μL/well, 0.5 μM IDE, 30 nM FL-6b, 4 ) the Multidrop Combi-nL Reagent Dispenser (Thermo Scientific).

    Article Title: High throughput flow cytometry identifies small molecule inhibitors for drug repurposing in T-ALL
    Article Snippet: Paragraph title: High Throughput Flow Cytometry Viability Testing ... An Echo® 555 Liquid Handler (Labcyte, San Jose, CA) was used both to create intermediate source plates and to add inhibitors to assay plates.

    Article Title: POSTER SESSION ABSTRACTS
    Article Snippet: The exceptional Zk Factor scores, show the flexibility and reliability of Promega assays in conjunction with Labcyte Echo and the Equator HTS from Deerac Fluidics in a high-throughput situation. .. Together with the Labcyte Echo 555 acoustic liquid handler and the Deerac Fluidics Equator HTS reagent dispenser, compounds can be screened in 1536-well format for effects on GPCRs.

    Article Title: Identification of Specific and Nonspecific Inhibitors of Bacillus anthracis Type III Pantothenate Kinase (PanK)
    Article Snippet: Paragraph title: High-throughput pantothenate kinase assay: ... For IC50 determinations of the test compounds,[ ] plates were predrugged using a Labcyte ECHO555 Liquid Handler.

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    Labcyte echo 555 acoustic liquid handler
    LOPAC 1280  library compound dispensing layout in a 1536-well assay plate. Compounds were dispensed using the Echo 555.
    Echo 555 Acoustic Liquid Handler, supplied by Labcyte, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/echo 555 acoustic liquid handler/product/Labcyte
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    echo 555 acoustic liquid handler - by Bioz Stars, 2020-02
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    Image Search Results


    LOPAC 1280  library compound dispensing layout in a 1536-well assay plate. Compounds were dispensed using the Echo 555.

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Miniaturized GPCR Signaling Studies in 1536-Well Format

    doi:

    Figure Lengend Snippet: LOPAC 1280 library compound dispensing layout in a 1536-well assay plate. Compounds were dispensed using the Echo 555.

    Article Snippet: Together with the Labcyte Echo 555 acoustic liquid handler and the Deerac Fluidics Equator HTS reagent dispenser, this setup can screen compounds in 96-, 384-, and 1536-well formats for their effects on GPCRs.

    Techniques:

    Secondary screening in a 1536-well assay plate layout for 12 hit compounds. Compounds were titrated using the Echo 555.

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Miniaturized GPCR Signaling Studies in 1536-Well Format

    doi:

    Figure Lengend Snippet: Secondary screening in a 1536-well assay plate layout for 12 hit compounds. Compounds were titrated using the Echo 555.

    Article Snippet: Together with the Labcyte Echo 555 acoustic liquid handler and the Deerac Fluidics Equator HTS reagent dispenser, this setup can screen compounds in 96-, 384-, and 1536-well formats for their effects on GPCRs.

    Techniques:

    A: Labcyte Echo 555 acoustic liquid handler. B: Schematic diagram of the acoustic drop ejection process. c: Time lapsed photographs of the 2.5-nL droplet ejection.

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: Miniaturized GPCR Signaling Studies in 1536-Well Format

    doi:

    Figure Lengend Snippet: A: Labcyte Echo 555 acoustic liquid handler. B: Schematic diagram of the acoustic drop ejection process. c: Time lapsed photographs of the 2.5-nL droplet ejection.

    Article Snippet: Together with the Labcyte Echo 555 acoustic liquid handler and the Deerac Fluidics Equator HTS reagent dispenser, this setup can screen compounds in 96-, 384-, and 1536-well formats for their effects on GPCRs.

    Techniques: