echo 555  (Labcyte)


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    Name:
    Echo 555 Liquid Handler
    Description:
    The Echo 555 Liquid Handler produce the high quality data with the highest throughput rate significantly reducing costs when using for sample management or high throughput plate preparation Our built in DMSO hydration measurements ensure accurate transfer and allow users to track compound library integrity
    Catalog Number:
    001-5234
    Price:
    None
    Category:
    Liquid handler
    Score:
    85
    Quantity:
    1
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    Structured Review

    Labcyte echo 555
    The Echo 555 Liquid Handler produce the high quality data with the highest throughput rate significantly reducing costs when using for sample management or high throughput plate preparation Our built in DMSO hydration measurements ensure accurate transfer and allow users to track compound library integrity
    https://www.bioz.com/result/echo 555/product/Labcyte
    Average 98 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    echo 555 - by Bioz Stars, 2019-10
    98/100 stars

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    Related Articles

    Luciferase:

    Article Title: Drug screening to target nuclear orphan receptor NR4A2 for cancer therapeutics
    Article Snippet: Labcyte Echo 555 was used to acoustically transfer the 5,384 FDA/Bioactive compounds (5 µM) and Life Chemical diversity set (15,040 compounds: 10 µM) to the assay plates. .. Labcyte Echo 555 was used to acoustically transfer the 5,384 FDA/Bioactive compounds (5 µM) and Life Chemical diversity set (15,040 compounds: 10 µM) to the assay plates.

    Mass Spectrometry:

    Article Title: Global survey of the immunomodulatory potential of common drugs
    Article Snippet: 50nl of selected screening compounds in DMSO, and DMSO controls, were transferred to Corning 384-well, tissue-culture treated clear bottom plates by a Labcyte Echo liquid handler attached to a Perkin Elmer high-content cell::explorer workstation. .. Screening compound libraries were obtained from the NIH Molecular Libraries Program, gifts from various groups (see ), or designed in-house.

    Stable Transfection:

    Article Title: Drug screening to target nuclear orphan receptor NR4A2 for cancer therapeutics
    Article Snippet: The primary screening was performed using a stably transfected H3118 cell line expressing luciferase reporter under the control of 5xNuRE regulatory elements. .. Labcyte Echo 555 was used to acoustically transfer the 5,384 FDA/Bioactive compounds (5 µM) and Life Chemical diversity set (15,040 compounds: 10 µM) to the assay plates.

    Construct:

    Article Title: Biochemical and biophysical characterization of four EphB kinase domains reveals contrasting thermodynamic, kinetic and inhibition profiles
    Article Snippet: Compounds were dosed into assay-ready plates using 11 half-log intervals, followed by the 12th point, which was a whole log interval from the 11th (Echo 555; Labcyte). .. Compounds were dosed into assay-ready plates using 11 half-log intervals, followed by the 12th point, which was a whole log interval from the 11th (Echo 555; Labcyte).

    Enzyme-linked Immunosorbent Assay:

    Article Title: A nonalcoholic fatty liver disease model in human induced pluripotent stem cell-derived hepatocytes, created by endoplasmic reticulum stress-induced steatosis
    Article Snippet: Albumin and urea production was quantified after 24 h and 48 h in fresh culture medium using human ELISA quantification kits (Abcam), according to the manufacturer's protocol, and read on an EnVision microplate reader (Perkin Elmer, Waltham, MA, USA). .. From each stock concentration, 2-fold serial dilutions were performed in 2.5 nl increments by acoustic dispensing using Echo 555 (Labcyte, Sunnyvale, CA, USA) and pre-incubated with cells for 24 h. The final concentration of DMSO did not exceed 0.25% in all wells.

    Microarray:

    Article Title: Correlating chemical sensitivity and basal gene expression reveals mechanism of action
    Article Snippet: To verify that CCLs tested in sensitivity profiling uniquely matched those used to generate microarray expression measurements, genomic DNA was extracted from 803/860 CCLs tested (93%) and used to genotype 96 SNPs using the Fluidigm 96.96 system; SNP calls were matched to a database of 1045 CCLs , . .. Compounds were added by acoustic transfer using a Labcyte Echo 555 (Labcyte Inc.) 24 hours after plating.

    Incubation:

    Article Title: Caspase-8 activation by TRAIL monotherapy predicts responses to IAPi and TRAIL combination treatment in breast cancer cell lines
    Article Snippet: For the high throughput screen, 500–2500 cells were seeded per well (the list of seeding density for individual cell lines is in ) of a 384-well Griener black clear bottomed tissue culture treated plate using Multidrop Combi (Thermo Scientific) and incubated overnight to allow cell attachment. .. The following day cells were treated with indicated compounds using acoustic dispenser Echo 555 (Labcyte, Sunnyvale, CA, USA) and cultivated for 5 days.

    Article Title: A high-throughput fluorescence polarization anisotropy assay for the 70N domain of Replication Protein A
    Article Snippet: 40 nL of compound was dispensed into a well using the ECHO 555 (Lab Labcyte), to which 6 μM RPA70N and 50 nM FITC-ATRIP in assay buffer were added to give a compound concentration of 10 μM in 0.01% DMSO with a total volume of 40 μL. .. Columns 1 and 24 of the plate contained 40 μL of 50 nM FITC-ATRIP alone, as a positive control (32 total wells) while columns 2 and 23 contained 40 μL of 6 μM RPA70N and 50 nM FITC-ATRIP in assay buffer (32 total wells) as a negative control.

    Article Title: Development and Validation of Fluorescence-Based and Automated Patch Clamp–Based Functional Assays for the Inward Rectifier Potassium Channel Kir4.1
    Article Snippet: Assay daughter plates were created using the ECHO 555 liquid handler (Labcyte), transferring 240 nL from the master plate to the daughter plate for each well followed by addition of 40 μL of AB resulting in antagonist compound concentration–response curves (CRCs) starting at 60 μM (2× final concentration). .. Assay daughter plates were created using the ECHO 555 liquid handler (Labcyte), transferring 240 nL from the master plate to the daughter plate for each well followed by addition of 40 μL of AB resulting in antagonist compound concentration–response curves (CRCs) starting at 60 μM (2× final concentration).

    Article Title: Biochemical and biophysical characterization of four EphB kinase domains reveals contrasting thermodynamic, kinetic and inhibition profiles
    Article Snippet: For the compound response testing, ATP and poly-(Glu:Tyr) were used at K m for each kinase ( ) and the kinase reaction was incubated for 40 min. .. Compounds were dosed into assay-ready plates using 11 half-log intervals, followed by the 12th point, which was a whole log interval from the 11th (Echo 555; Labcyte).

    Article Title: Release of 50 new, drug-like compounds and their computational target predictions for open source anti-tubercular drug discovery
    Article Snippet: The screening compounds were added to the plates as a 50 nL solution in neat DMSO (Sigma, D8418) prior to addition of the assay components by using an Echo 555 instrument (Labcyte Inc). .. The screening compounds were added to the plates as a 50 nL solution in neat DMSO (Sigma, D8418) prior to addition of the assay components by using an Echo 555 instrument (Labcyte Inc).

    Article Title: Global survey of the immunomodulatory potential of common drugs
    Article Snippet: 50nl of selected screening compounds in DMSO, and DMSO controls, were transferred to Corning 384-well, tissue-culture treated clear bottom plates by a Labcyte Echo liquid handler attached to a Perkin Elmer high-content cell::explorer workstation. .. 50nl of selected screening compounds in DMSO, and DMSO controls, were transferred to Corning 384-well, tissue-culture treated clear bottom plates by a Labcyte Echo liquid handler attached to a Perkin Elmer high-content cell::explorer workstation.

    Article Title: A Novel High-Throughput Cell-Based Assay Aimed at Identifying Inhibitors of DNA Metabolism in Bacteria
    Article Snippet: Test compounds from the AstraZeneca compound collection were dissolved in DMSO to form stock solutions (10 mM) and acoustically dispensed using an Echo 555 instrument (Labcyte, Sunnyvale, CA) into 384-well, black high-binding plates (Greiner Bio One, Frickenhausen, Germany). .. Test compounds from the AstraZeneca compound collection were dissolved in DMSO to form stock solutions (10 mM) and acoustically dispensed using an Echo 555 instrument (Labcyte, Sunnyvale, CA) into 384-well, black high-binding plates (Greiner Bio One, Frickenhausen, Germany).

    Article Title: Acoustic Droplet Ejection Technology and Its Application in High-Throughput RNA Interference Screening
    Article Snippet: Screens were performed in single siRNA/well format. siRNAs (150 nL of a 1 μM stock; 0.15 pmol/well) were dispensed into 384-well assay plates (Corning 3712; Corning, NY) using an Echo 555 Series Liquid Handler (Labcyte) or, where indicated, a Biomek FX (Beckman Coulter, Fullerton, CA). .. Screens were performed in single siRNA/well format. siRNAs (150 nL of a 1 μM stock; 0.15 pmol/well) were dispensed into 384-well assay plates (Corning 3712; Corning, NY) using an Echo 555 Series Liquid Handler (Labcyte) or, where indicated, a Biomek FX (Beckman Coulter, Fullerton, CA).

    Article Title: Gβγ directly modulates vesicle fusion by competing with synaptotagmin for binding to neuronal SNARE proteins embedded in membranes
    Article Snippet: Peptide stocks in DMSO were spotted onto 384-well white OptiPlates (PerkinElmer Life Sciences) at concentration ranges of 1 n m to 100 μ m using a Labcyte Echo 555 Omics acoustic liquid handler (Labcyte, Sunnyvale, CA), with DMSO being back-added to a final concentration of 0.1%. .. After 5 min, 1μl of biotinylated SNAP25 was added to a final concentration of 20 n m .

    Activity Assay:

    Article Title: Identification and characterization of aspartyl-tRNA synthetase inhibitors against Mycobacterium tuberculosis by an integrated whole-cell target-based approach
    Article Snippet: Stock compounds were dispensed with the Echo 555 (Labcyte) into black bottom 384 plates (Greiner 781101) and were stored at −80 °C until needed. .. Similar amounts of DMSO (1%) were added into control wells to check for absence of solvent-related inhibition.

    Article Title: A Novel High-Throughput Cell-Based Assay Aimed at Identifying Inhibitors of DNA Metabolism in Bacteria
    Article Snippet: Test compounds from the AstraZeneca compound collection were dissolved in DMSO to form stock solutions (10 mM) and acoustically dispensed using an Echo 555 instrument (Labcyte, Sunnyvale, CA) into 384-well, black high-binding plates (Greiner Bio One, Frickenhausen, Germany). .. Test compounds from the AstraZeneca compound collection were dissolved in DMSO to form stock solutions (10 mM) and acoustically dispensed using an Echo 555 instrument (Labcyte, Sunnyvale, CA) into 384-well, black high-binding plates (Greiner Bio One, Frickenhausen, Germany).

    Expressing:

    Article Title: Drug screening to target nuclear orphan receptor NR4A2 for cancer therapeutics
    Article Snippet: Labcyte Echo 555 was used to acoustically transfer the 5,384 FDA/Bioactive compounds (5 µM) and Life Chemical diversity set (15,040 compounds: 10 µM) to the assay plates. .. Labcyte Echo 555 was used to acoustically transfer the 5,384 FDA/Bioactive compounds (5 µM) and Life Chemical diversity set (15,040 compounds: 10 µM) to the assay plates.

    Article Title: Correlating chemical sensitivity and basal gene expression reveals mechanism of action
    Article Snippet: To verify that CCLs tested in sensitivity profiling uniquely matched those used to generate microarray expression measurements, genomic DNA was extracted from 803/860 CCLs tested (93%) and used to genotype 96 SNPs using the Fluidigm 96.96 system; SNP calls were matched to a database of 1045 CCLs , . .. Compounds were added by acoustic transfer using a Labcyte Echo 555 (Labcyte Inc.) 24 hours after plating.

    Release Assay:

    Article Title: Identification and characterization of aspartyl-tRNA synthetase inhibitors against Mycobacterium tuberculosis by an integrated whole-cell target-based approach
    Article Snippet: A number of structurally related analogues to GSK97C, GSK93A and GSK87A from the GSK TB box collection were evaluated in a dose-response manner (0.1–100 μM) in a 384-well black-bottom microplate-adapted ATP release assay optimised for HTS purposes. .. Stock compounds were dispensed with the Echo 555 (Labcyte) into black bottom 384 plates (Greiner 781101) and were stored at −80 °C until needed.

    Transfection:

    Article Title: Drug screening to target nuclear orphan receptor NR4A2 for cancer therapeutics
    Article Snippet: The primary screening was performed using a stably transfected H3118 cell line expressing luciferase reporter under the control of 5xNuRE regulatory elements. .. Labcyte Echo 555 was used to acoustically transfer the 5,384 FDA/Bioactive compounds (5 µM) and Life Chemical diversity set (15,040 compounds: 10 µM) to the assay plates.

    Article Title: Acoustic Droplet Ejection Technology and Its Application in High-Throughput RNA Interference Screening
    Article Snippet: Paragraph title: siRNA Transfection ... Screens were performed in single siRNA/well format. siRNAs (150 nL of a 1 μM stock; 0.15 pmol/well) were dispensed into 384-well assay plates (Corning 3712; Corning, NY) using an Echo 555 Series Liquid Handler (Labcyte) or, where indicated, a Biomek FX (Beckman Coulter, Fullerton, CA).

    Transferring:

    Article Title: Development and Validation of Fluorescence-Based and Automated Patch Clamp–Based Functional Assays for the Inward Rectifier Potassium Channel Kir4.1
    Article Snippet: Compound master plates were created by serially diluting compounds 1:3 from 10 mM stock in 100% DMSO using the BRAVO liquid handler (Agilent Technologies). .. Assay daughter plates were created using the ECHO 555 liquid handler (Labcyte), transferring 240 nL from the master plate to the daughter plate for each well followed by addition of 40 μL of AB resulting in antagonist compound concentration–response curves (CRCs) starting at 60 μM (2× final concentration). .. Concentration–response series were done in triplicate in the daughter plate.

    Article Title: Eliciting Renal Failure in Mosquitoes with a Small-Molecule Inhibitor of Inward-Rectifying Potassium Channels
    Article Snippet: Compound master antagonist plates were created by serial diluting compounds 1∶3 from 30 mM stocks in 100% DMSO using the BRAVO liquid handler (Agilent Technologies, Santa Clara, CA). .. Assay daughter plates were created using the ECHO 555 liquid hander (Labcyte, Sunnyvale, CA), transferring 240 nl from the master plate to the daughter plate for each well followed by addition of 40 µl of assay buffer resulting in antagonist compound concentration response curves starting at 200 µM (2× final concentration). .. Tl+ stimulus buffer contained (in mM): 125 mM sodium bicarbonate (added fresh the morning of the experiment), 1 mM magnesium sulfate, 1.8 mM calcium sulfate, 5 mM glucose, 12 mM Tl+ sulfate, and 10 mM HEPES, pH 7.3 at 5× the final concentration to be assayed.

    Cell Culture:

    Article Title: A nonalcoholic fatty liver disease model in human induced pluripotent stem cell-derived hepatocytes, created by endoplasmic reticulum stress-induced steatosis
    Article Snippet: Paragraph title: hiPSC-Hep cell culture and compound treatment ... From each stock concentration, 2-fold serial dilutions were performed in 2.5 nl increments by acoustic dispensing using Echo 555 (Labcyte, Sunnyvale, CA, USA) and pre-incubated with cells for 24 h. The final concentration of DMSO did not exceed 0.25% in all wells.

    Generated:

    Article Title: Caspase-8 activation by TRAIL monotherapy predicts responses to IAPi and TRAIL combination treatment in breast cancer cell lines
    Article Snippet: The following day cells were treated with indicated compounds using acoustic dispenser Echo 555 (Labcyte, Sunnyvale, CA, USA) and cultivated for 5 days. .. Subsequently, 10 μ l of 0.25% saponin in TBS/5mM EDTA was added to each well, incubated at room temperature for 16 h and re-read using the Acumen as previously for the dead cells read.

    Inhibition:

    Article Title: Biochemical and biophysical characterization of four EphB kinase domains reveals contrasting thermodynamic, kinetic and inhibition profiles
    Article Snippet: Compounds were dosed into assay-ready plates using 11 half-log intervals, followed by the 12th point, which was a whole log interval from the 11th (Echo 555; Labcyte). .. Compounds were dosed into assay-ready plates using 11 half-log intervals, followed by the 12th point, which was a whole log interval from the 11th (Echo 555; Labcyte).

    Article Title: Identification and characterization of aspartyl-tRNA synthetase inhibitors against Mycobacterium tuberculosis by an integrated whole-cell target-based approach
    Article Snippet: Stock compounds were dispensed with the Echo 555 (Labcyte) into black bottom 384 plates (Greiner 781101) and were stored at −80 °C until needed. .. Stock compounds were dispensed with the Echo 555 (Labcyte) into black bottom 384 plates (Greiner 781101) and were stored at −80 °C until needed.

    Imaging:

    Article Title: A nonalcoholic fatty liver disease model in human induced pluripotent stem cell-derived hepatocytes, created by endoplasmic reticulum stress-induced steatosis
    Article Snippet: Nuclei were labeled with 10 µg/ml Hoechst 33342 (Thermo Fisher Scientific) and plates were imaged and analyzed as described below in the ‘High-content imaging and quantification’ section. .. From each stock concentration, 2-fold serial dilutions were performed in 2.5 nl increments by acoustic dispensing using Echo 555 (Labcyte, Sunnyvale, CA, USA) and pre-incubated with cells for 24 h. The final concentration of DMSO did not exceed 0.25% in all wells.

    Amplified Luminescent Proximity Homogenous Assay:

    Article Title: Gβγ directly modulates vesicle fusion by competing with synaptotagmin for binding to neuronal SNARE proteins embedded in membranes
    Article Snippet: Paragraph title: Alphascreen competition-binding assays ... Peptide stocks in DMSO were spotted onto 384-well white OptiPlates (PerkinElmer Life Sciences) at concentration ranges of 1 n m to 100 μ m using a Labcyte Echo 555 Omics acoustic liquid handler (Labcyte, Sunnyvale, CA), with DMSO being back-added to a final concentration of 0.1%.

    Screening Assay:

    Article Title: Identification and characterization of aspartyl-tRNA synthetase inhibitors against Mycobacterium tuberculosis by an integrated whole-cell target-based approach
    Article Snippet: Paragraph title: Medium-throughput screening assay of a series of analogues in a tRNA-independent biochemical assay ... Stock compounds were dispensed with the Echo 555 (Labcyte) into black bottom 384 plates (Greiner 781101) and were stored at −80 °C until needed.

    Article Title: Acoustic Droplet Ejection Technology and Its Application in High-Throughput RNA Interference Screening
    Article Snippet: Screens were performed in single siRNA/well format. siRNAs (150 nL of a 1 μM stock; 0.15 pmol/well) were dispensed into 384-well assay plates (Corning 3712; Corning, NY) using an Echo 555 Series Liquid Handler (Labcyte) or, where indicated, a Biomek FX (Beckman Coulter, Fullerton, CA). .. Screens were performed in single siRNA/well format. siRNAs (150 nL of a 1 μM stock; 0.15 pmol/well) were dispensed into 384-well assay plates (Corning 3712; Corning, NY) using an Echo 555 Series Liquid Handler (Labcyte) or, where indicated, a Biomek FX (Beckman Coulter, Fullerton, CA).

    Fluorescence:

    Article Title: A high-throughput fluorescence polarization anisotropy assay for the 70N domain of Replication Protein A
    Article Snippet: 40 nL of compound was dispensed into a well using the ECHO 555 (Lab Labcyte), to which 6 μM RPA70N and 50 nM FITC-ATRIP in assay buffer were added to give a compound concentration of 10 μM in 0.01% DMSO with a total volume of 40 μL. .. Columns 1 and 24 of the plate contained 40 μL of 50 nM FITC-ATRIP alone, as a positive control (32 total wells) while columns 2 and 23 contained 40 μL of 6 μM RPA70N and 50 nM FITC-ATRIP in assay buffer (32 total wells) as a negative control.

    Article Title: Identification and characterization of aspartyl-tRNA synthetase inhibitors against Mycobacterium tuberculosis by an integrated whole-cell target-based approach
    Article Snippet: Stock compounds were dispensed with the Echo 555 (Labcyte) into black bottom 384 plates (Greiner 781101) and were stored at −80 °C until needed. .. Stock compounds were dispensed with the Echo 555 (Labcyte) into black bottom 384 plates (Greiner 781101) and were stored at −80 °C until needed.

    Article Title: A Novel High-Throughput Cell-Based Assay Aimed at Identifying Inhibitors of DNA Metabolism in Bacteria
    Article Snippet: Test compounds from the AstraZeneca compound collection were dissolved in DMSO to form stock solutions (10 mM) and acoustically dispensed using an Echo 555 instrument (Labcyte, Sunnyvale, CA) into 384-well, black high-binding plates (Greiner Bio One, Frickenhausen, Germany). .. Test compounds from the AstraZeneca compound collection were dissolved in DMSO to form stock solutions (10 mM) and acoustically dispensed using an Echo 555 instrument (Labcyte, Sunnyvale, CA) into 384-well, black high-binding plates (Greiner Bio One, Frickenhausen, Germany).

    Labeling:

    Article Title: A nonalcoholic fatty liver disease model in human induced pluripotent stem cell-derived hepatocytes, created by endoplasmic reticulum stress-induced steatosis
    Article Snippet: Nuclei were labeled with 10 µg/ml Hoechst 33342 (Thermo Fisher Scientific) and plates were imaged and analyzed as described below in the ‘High-content imaging and quantification’ section. .. From each stock concentration, 2-fold serial dilutions were performed in 2.5 nl increments by acoustic dispensing using Echo 555 (Labcyte, Sunnyvale, CA, USA) and pre-incubated with cells for 24 h. The final concentration of DMSO did not exceed 0.25% in all wells.

    Titration:

    Article Title: A nonalcoholic fatty liver disease model in human induced pluripotent stem cell-derived hepatocytes, created by endoplasmic reticulum stress-induced steatosis
    Article Snippet: For dose-response titration, OCA (Abcam) stocks at 20 mM and 0.312 mM in 100% dimethyl sulfoxide (DMSO) were used. .. From each stock concentration, 2-fold serial dilutions were performed in 2.5 nl increments by acoustic dispensing using Echo 555 (Labcyte, Sunnyvale, CA, USA) and pre-incubated with cells for 24 h. The final concentration of DMSO did not exceed 0.25% in all wells.

    Article Title: Inhibitor of intramembrane protease RseP blocks the σE response causing lethal accumulation of unfolded outer membrane proteins
    Article Snippet: Test compounds from a subset of the Merck & Co., Inc., compound collection ( ) were prepared in master plates at 2 mM in 100% DMSO, and 0.5 µL of each compound was dispensed on the surface of the agar plates using a Labcyte Echo 555 liquid handler. .. After the compound droplets were absorbed, the plates were inverted, sealed in plastic bags, and incubated at 37 °C for approximately 18 h. To identify compounds of interest, the plates were photographed with illumination through the plate, and images of plates from matching compound sets were compared for differences in the size of the ZOI at each spot.

    Purification:

    Article Title: Global survey of the immunomodulatory potential of common drugs
    Article Snippet: 50nl of selected screening compounds in DMSO, and DMSO controls, were transferred to Corning 384-well, tissue-culture treated clear bottom plates by a Labcyte Echo liquid handler attached to a Perkin Elmer high-content cell::explorer workstation. .. 50nl of selected screening compounds in DMSO, and DMSO controls, were transferred to Corning 384-well, tissue-culture treated clear bottom plates by a Labcyte Echo liquid handler attached to a Perkin Elmer high-content cell::explorer workstation.

    Article Title: Gβγ directly modulates vesicle fusion by competing with synaptotagmin for binding to neuronal SNARE proteins embedded in membranes
    Article Snippet: An EC80 concentration of 180 n m purified His6 -Gβ1 γ2 was made in assay buffer. .. Peptide stocks in DMSO were spotted onto 384-well white OptiPlates (PerkinElmer Life Sciences) at concentration ranges of 1 n m to 100 μ m using a Labcyte Echo 555 Omics acoustic liquid handler (Labcyte, Sunnyvale, CA), with DMSO being back-added to a final concentration of 0.1%.

    Software:

    Article Title: Biochemical and biophysical characterization of four EphB kinase domains reveals contrasting thermodynamic, kinetic and inhibition profiles
    Article Snippet: Values for K m , V max and k cat were calculated using Prism analysis software (version 5; GraphPad) by Michaelis–Menten nonlinear regression analysis. .. Compounds were dosed into assay-ready plates using 11 half-log intervals, followed by the 12th point, which was a whole log interval from the 11th (Echo 555; Labcyte).

    Article Title: A Novel High-Throughput Cell-Based Assay Aimed at Identifying Inhibitors of DNA Metabolism in Bacteria
    Article Snippet: Test compounds from the AstraZeneca compound collection were dissolved in DMSO to form stock solutions (10 mM) and acoustically dispensed using an Echo 555 instrument (Labcyte, Sunnyvale, CA) into 384-well, black high-binding plates (Greiner Bio One, Frickenhausen, Germany). .. The plates were incubated at 37°C for 16 h before fluorescence was measured using a Pherastar plate reader (BMG Labtech, Ortenberg, Germany) with excitation at 485 nm and emission at 535 nm.

    Cell Attachment Assay:

    Article Title: Caspase-8 activation by TRAIL monotherapy predicts responses to IAPi and TRAIL combination treatment in breast cancer cell lines
    Article Snippet: For the high throughput screen, 500–2500 cells were seeded per well (the list of seeding density for individual cell lines is in ) of a 384-well Griener black clear bottomed tissue culture treated plate using Multidrop Combi (Thermo Scientific) and incubated overnight to allow cell attachment. .. The following day cells were treated with indicated compounds using acoustic dispenser Echo 555 (Labcyte, Sunnyvale, CA, USA) and cultivated for 5 days.

    HTS Assay:

    Article Title: Release of 50 new, drug-like compounds and their computational target predictions for open source anti-tubercular drug discovery
    Article Snippet: The HTS assay was carried out in 1536-well sterile plates (Greiner, 782074). .. The screening compounds were added to the plates as a 50 nL solution in neat DMSO (Sigma, D8418) prior to addition of the assay components by using an Echo 555 instrument (Labcyte Inc).

    Article Title: A BSL-4 High-Throughput Screen Identifies Sulfonamide Inhibitors of Nipah Virus
    Article Snippet: A subset (10,080 compounds) of the 30K compound chemical diversity library provided by Enamine Ltd. (Kiev, Ukraine) was used for HTS assay validation. .. Compounds (solubilized in 100% DMSO) at 5 mg/mL stock concentration were dispensed to the assay plates in 75 nL volumes using an Echo 555™ noncontact liquid dispenser (LabCyte, Sunnyvale, CA).

    Evaporation:

    Article Title: Release of 50 new, drug-like compounds and their computational target predictions for open source anti-tubercular drug discovery
    Article Snippet: The screening compounds were added to the plates as a 50 nL solution in neat DMSO (Sigma, D8418) prior to addition of the assay components by using an Echo 555 instrument (Labcyte Inc). .. The screening compounds were added to the plates as a 50 nL solution in neat DMSO (Sigma, D8418) prior to addition of the assay components by using an Echo 555 instrument (Labcyte Inc).

    Concentration Assay:

    Article Title: A high-throughput fluorescence polarization anisotropy assay for the 70N domain of Replication Protein A
    Article Snippet: The SPECTRUM collection (Microsource Discovery Systems Inc.) of 2000 compounds was distributed into seven 384-well plates. .. 40 nL of compound was dispensed into a well using the ECHO 555 (Lab Labcyte), to which 6 μM RPA70N and 50 nM FITC-ATRIP in assay buffer were added to give a compound concentration of 10 μM in 0.01% DMSO with a total volume of 40 μL. .. Columns 1 and 24 of the plate contained 40 μL of 50 nM FITC-ATRIP alone, as a positive control (32 total wells) while columns 2 and 23 contained 40 μL of 6 μM RPA70N and 50 nM FITC-ATRIP in assay buffer (32 total wells) as a negative control.

    Article Title: Development and Validation of Fluorescence-Based and Automated Patch Clamp–Based Functional Assays for the Inward Rectifier Potassium Channel Kir4.1
    Article Snippet: Compound master plates were created by serially diluting compounds 1:3 from 10 mM stock in 100% DMSO using the BRAVO liquid handler (Agilent Technologies). .. Assay daughter plates were created using the ECHO 555 liquid handler (Labcyte), transferring 240 nL from the master plate to the daughter plate for each well followed by addition of 40 μL of AB resulting in antagonist compound concentration–response curves (CRCs) starting at 60 μM (2× final concentration). .. Concentration–response series were done in triplicate in the daughter plate.

    Article Title: A nonalcoholic fatty liver disease model in human induced pluripotent stem cell-derived hepatocytes, created by endoplasmic reticulum stress-induced steatosis
    Article Snippet: For dose-response titration, OCA (Abcam) stocks at 20 mM and 0.312 mM in 100% dimethyl sulfoxide (DMSO) were used. .. From each stock concentration, 2-fold serial dilutions were performed in 2.5 nl increments by acoustic dispensing using Echo 555 (Labcyte, Sunnyvale, CA, USA) and pre-incubated with cells for 24 h. The final concentration of DMSO did not exceed 0.25% in all wells. .. Data were fit to nonlinear regression with variable slope to determine half-maximal effective concentration (EC50 ) values, or to one-way analysis of variance (ANOVA) to determine column statistics between treatment groups using Prism 7.0 software (GraphPad, San Diego, CA, USA).

    Article Title: Biochemical and biophysical characterization of four EphB kinase domains reveals contrasting thermodynamic, kinetic and inhibition profiles
    Article Snippet: The K m for poly-(Glu:Tyr) was measured using a concentration range of 0–10 mg/ml poly-(Glu:Tyr) at a fixed ATP concentration of 5 mM. .. Compounds were dosed into assay-ready plates using 11 half-log intervals, followed by the 12th point, which was a whole log interval from the 11th (Echo 555; Labcyte).

    Article Title: Correlating chemical sensitivity and basal gene expression reveals mechanism of action
    Article Snippet: Compounds were added by acoustic transfer using a Labcyte Echo 555 (Labcyte Inc.) 24 hours after plating. .. The effects of small molecules were measured over a 16-point concentration range (two-fold dilution) in duplicate.

    Article Title: Global survey of the immunomodulatory potential of common drugs
    Article Snippet: 50nl of selected screening compounds in DMSO, and DMSO controls, were transferred to Corning 384-well, tissue-culture treated clear bottom plates by a Labcyte Echo liquid handler attached to a Perkin Elmer high-content cell::explorer workstation. .. 50nl of selected screening compounds in DMSO, and DMSO controls, were transferred to Corning 384-well, tissue-culture treated clear bottom plates by a Labcyte Echo liquid handler attached to a Perkin Elmer high-content cell::explorer workstation.

    Article Title: A BSL-4 High-Throughput Screen Identifies Sulfonamide Inhibitors of Nipah Virus
    Article Snippet: A subset (10,080 compounds) of the 30K compound chemical diversity library provided by Enamine Ltd. (Kiev, Ukraine) was used for HTS assay validation. .. Compounds (solubilized in 100% DMSO) at 5 mg/mL stock concentration were dispensed to the assay plates in 75 nL volumes using an Echo 555™ noncontact liquid dispenser (LabCyte, Sunnyvale, CA). .. The final compound concentration used in primary screening was 12.5 μg/mL, with a final DMSO concentration of 0.25%.

    Article Title: Eliciting Renal Failure in Mosquitoes with a Small-Molecule Inhibitor of Inward-Rectifying Potassium Channels
    Article Snippet: Compound master antagonist plates were created by serial diluting compounds 1∶3 from 30 mM stocks in 100% DMSO using the BRAVO liquid handler (Agilent Technologies, Santa Clara, CA). .. Assay daughter plates were created using the ECHO 555 liquid hander (Labcyte, Sunnyvale, CA), transferring 240 nl from the master plate to the daughter plate for each well followed by addition of 40 µl of assay buffer resulting in antagonist compound concentration response curves starting at 200 µM (2× final concentration). .. Tl+ stimulus buffer contained (in mM): 125 mM sodium bicarbonate (added fresh the morning of the experiment), 1 mM magnesium sulfate, 1.8 mM calcium sulfate, 5 mM glucose, 12 mM Tl+ sulfate, and 10 mM HEPES, pH 7.3 at 5× the final concentration to be assayed.

    Article Title: Gβγ directly modulates vesicle fusion by competing with synaptotagmin for binding to neuronal SNARE proteins embedded in membranes
    Article Snippet: An EC80 concentration of 180 n m purified His6 -Gβ1 γ2 was made in assay buffer. .. Peptide stocks in DMSO were spotted onto 384-well white OptiPlates (PerkinElmer Life Sciences) at concentration ranges of 1 n m to 100 μ m using a Labcyte Echo 555 Omics acoustic liquid handler (Labcyte, Sunnyvale, CA), with DMSO being back-added to a final concentration of 0.1%. .. 4 μl of Gβ1 γ2 solution was incubated with peptide for 5 min while shaking.

    Cell Counting:

    Article Title: Caspase-8 activation by TRAIL monotherapy predicts responses to IAPi and TRAIL combination treatment in breast cancer cell lines
    Article Snippet: The following day cells were treated with indicated compounds using acoustic dispenser Echo 555 (Labcyte, Sunnyvale, CA, USA) and cultivated for 5 days. .. Heatmaps were generated for visual purposes.

    High Throughput Screening Assay:

    Article Title: Caspase-8 activation by TRAIL monotherapy predicts responses to IAPi and TRAIL combination treatment in breast cancer cell lines
    Article Snippet: Paragraph title: 384-well format proliferation-cell death assay and high throughput screen ... The following day cells were treated with indicated compounds using acoustic dispenser Echo 555 (Labcyte, Sunnyvale, CA, USA) and cultivated for 5 days.

    Article Title: A high-throughput fluorescence polarization anisotropy assay for the 70N domain of Replication Protein A
    Article Snippet: Paragraph title: High Throughput Screening ... 40 nL of compound was dispensed into a well using the ECHO 555 (Lab Labcyte), to which 6 μM RPA70N and 50 nM FITC-ATRIP in assay buffer were added to give a compound concentration of 10 μM in 0.01% DMSO with a total volume of 40 μL.

    Article Title: Drug screening to target nuclear orphan receptor NR4A2 for cancer therapeutics
    Article Snippet: Paragraph title: High throughput screening ... Labcyte Echo 555 was used to acoustically transfer the 5,384 FDA/Bioactive compounds (5 µM) and Life Chemical diversity set (15,040 compounds: 10 µM) to the assay plates.

    other:

    Article Title: Increasing procaspase 8 expression using repurposed drugs to induce HIV infected cell death in ex vivo patient cells
    Article Snippet: Authors thank Sanford Burnham Prebys Medical Discovery Institute (SBP) for the gift of microtiter plates pre-spotted (50 nL) with the Prestwick Chemical Library as prepared by Fu-Yue Zeng, Ph.D. of the Conrad Prebys Center for Chemical Genomics at SBP using their Echo 555 acoustic droplet ejector (Labcyte, Sunnyvale, CA).

    Article Title: Release of 50 new, drug-like compounds and their computational target predictions for open source anti-tubercular drug discovery
    Article Snippet: Prior to addition of the cell suspension, the screening compounds (250 nL) were dispensed into the plates with an Echo 555 instrument.

    Article Title: Unravelling the rate of action of hits in the Leishmania donovani box using standard drugs amphotericin B and miltefosine
    Article Snippet: Pre-dispensed 384-wells assay plates (Greiner μclear black) were prepared by adding 250 nL of compounds dissolved in 100% DMSO to each well by using an Echo® liquid handler (Labcyte Inc.).

    Hood:

    Article Title: Inhibitor of intramembrane protease RseP blocks the σE response causing lethal accumulation of unfolded outer membrane proteins
    Article Snippet: Test compounds from a subset of the Merck & Co., Inc., compound collection ( ) were prepared in master plates at 2 mM in 100% DMSO, and 0.5 µL of each compound was dispensed on the surface of the agar plates using a Labcyte Echo 555 liquid handler. .. Test compounds from a subset of the Merck & Co., Inc., compound collection ( ) were prepared in master plates at 2 mM in 100% DMSO, and 0.5 µL of each compound was dispensed on the surface of the agar plates using a Labcyte Echo 555 liquid handler.

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    Labcyte echo 555
    Echo 555, supplied by Labcyte, used in various techniques. Bioz Stars score: 98/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/echo 555/product/Labcyte
    Average 98 stars, based on 24 article reviews
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    echo 555 - by Bioz Stars, 2019-10
    98/100 stars
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