echo 550 acoustic liquid handler  (Labcyte)


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    Name:
    Echo 550 Liquid Handler
    Description:
    With the patented Dynamic Fluid Analysis technology the Echo 550 eliminates the need for calibration to run an assay These models enable high speed contamination free transfer of cDNA siRNA vectors proteins and crystallography reagents in nanoliter increments
    Catalog Number:
    001-2000
    Price:
    None
    Category:
    Liquid handler
    Quantity:
    1
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    Structured Review

    Labcyte echo 550 acoustic liquid handler
    Plan diagram of Eve's laboratory robotic system . Layout diagram of Eve's laboratory robotic system, comprising: [1] Labcyte <t>Echo</t> 550 acoustic liquid handler, [2] BMG Pherastar reader, [3] MDS ImageXpress Micro cellular imager, [4] BMG Polarstar reader, [5] Cytomat 2C435 incubator, [6] Cytomat 6003 dry store, [7] FluidX DC-96pro capper/recapper, [8] two Variomag teleshake plate shakers and two Metrologic Orbit 1D barcode readers, [9] Cytomat linear actuator track, [10] robot plinth holding two Mitsubishi robot arms; models RV-3SJB and RV-3SJ, [11] FluidX Xtr-96 tube rack 2D barcode scanner, [12] Agilent (Velocity 11) Bravo liquid handler, [13] Thermo Combi-nL multidrop, [14] two Thermo Combi multidrops, and [15] consumables stacks for microplates, tube racks and tips. There are also two computers controlling the robotics, plus a networked computer server which runs all the other code vital to Eve's function: the chemistry knowledge base, QSARs and hypothesis generation, experiment planning, results relational database, data analysis etc. (not shown).
    With the patented Dynamic Fluid Analysis technology the Echo 550 eliminates the need for calibration to run an assay These models enable high speed contamination free transfer of cDNA siRNA vectors proteins and crystallography reagents in nanoliter increments
    https://www.bioz.com/result/echo 550 acoustic liquid handler/product/Labcyte
    Average 95 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    echo 550 acoustic liquid handler - by Bioz Stars, 2020-02
    95/100 stars

    Images

    1) Product Images from "Towards Robot Scientists for autonomous scientific discovery"

    Article Title: Towards Robot Scientists for autonomous scientific discovery

    Journal: Automated Experimentation

    doi: 10.1186/1759-4499-2-1

    Plan diagram of Eve's laboratory robotic system . Layout diagram of Eve's laboratory robotic system, comprising: [1] Labcyte Echo 550 acoustic liquid handler, [2] BMG Pherastar reader, [3] MDS ImageXpress Micro cellular imager, [4] BMG Polarstar reader, [5] Cytomat 2C435 incubator, [6] Cytomat 6003 dry store, [7] FluidX DC-96pro capper/recapper, [8] two Variomag teleshake plate shakers and two Metrologic Orbit 1D barcode readers, [9] Cytomat linear actuator track, [10] robot plinth holding two Mitsubishi robot arms; models RV-3SJB and RV-3SJ, [11] FluidX Xtr-96 tube rack 2D barcode scanner, [12] Agilent (Velocity 11) Bravo liquid handler, [13] Thermo Combi-nL multidrop, [14] two Thermo Combi multidrops, and [15] consumables stacks for microplates, tube racks and tips. There are also two computers controlling the robotics, plus a networked computer server which runs all the other code vital to Eve's function: the chemistry knowledge base, QSARs and hypothesis generation, experiment planning, results relational database, data analysis etc. (not shown).
    Figure Legend Snippet: Plan diagram of Eve's laboratory robotic system . Layout diagram of Eve's laboratory robotic system, comprising: [1] Labcyte Echo 550 acoustic liquid handler, [2] BMG Pherastar reader, [3] MDS ImageXpress Micro cellular imager, [4] BMG Polarstar reader, [5] Cytomat 2C435 incubator, [6] Cytomat 6003 dry store, [7] FluidX DC-96pro capper/recapper, [8] two Variomag teleshake plate shakers and two Metrologic Orbit 1D barcode readers, [9] Cytomat linear actuator track, [10] robot plinth holding two Mitsubishi robot arms; models RV-3SJB and RV-3SJ, [11] FluidX Xtr-96 tube rack 2D barcode scanner, [12] Agilent (Velocity 11) Bravo liquid handler, [13] Thermo Combi-nL multidrop, [14] two Thermo Combi multidrops, and [15] consumables stacks for microplates, tube racks and tips. There are also two computers controlling the robotics, plus a networked computer server which runs all the other code vital to Eve's function: the chemistry knowledge base, QSARs and hypothesis generation, experiment planning, results relational database, data analysis etc. (not shown).

    Techniques Used:

    2) Product Images from "Discovery of Bile Salt Hydrolase Inhibitors Using an Efficient High-Throughput Screening System"

    Article Title: Discovery of Bile Salt Hydrolase Inhibitors Using an Efficient High-Throughput Screening System

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0085344

    Representative result of high-throughput screening of BSH inhibitors. (A) Plate layout for screening BSH inhibitors. Pink boxes (columns 3–22) indicate test wells that contain library compounds of interest, BSH, and reaction mix containing substrate. Library compounds were shot into the well bottom using Echo 550/555 and enzyme and reaction mix were added using Multidrop Combi. Controls were added manually to the side wells (columns 1–2 and 23–24): blue boxes indicate activity controls (BSH, reaction mix containing substrate, and solvent DMSO), yellow boxes correspond to inhibition controls (BSH, reaction mix containing substrate, and NaIO 3 ), and white boxes are negative controls with no BSH added but include reaction mix as well as substrate. (B) The HTS results represented by one 384-well plate. Control wells indicate the assay proceeded normally. The wells in columns 3–22 that appeared clear, regardless of alternative color due to compound, and had low absorbance readings were considered hits (putative BSH inhibitors).
    Figure Legend Snippet: Representative result of high-throughput screening of BSH inhibitors. (A) Plate layout for screening BSH inhibitors. Pink boxes (columns 3–22) indicate test wells that contain library compounds of interest, BSH, and reaction mix containing substrate. Library compounds were shot into the well bottom using Echo 550/555 and enzyme and reaction mix were added using Multidrop Combi. Controls were added manually to the side wells (columns 1–2 and 23–24): blue boxes indicate activity controls (BSH, reaction mix containing substrate, and solvent DMSO), yellow boxes correspond to inhibition controls (BSH, reaction mix containing substrate, and NaIO 3 ), and white boxes are negative controls with no BSH added but include reaction mix as well as substrate. (B) The HTS results represented by one 384-well plate. Control wells indicate the assay proceeded normally. The wells in columns 3–22 that appeared clear, regardless of alternative color due to compound, and had low absorbance readings were considered hits (putative BSH inhibitors).

    Techniques Used: High Throughput Screening Assay, Activity Assay, Inhibition

    Related Articles

    Positive Control:

    Article Title: Targeting Epithelial-Mesenchymal Transition for Identification of Inhibitors for Pancreatic Cancer Cell Invasion and Tumor Spheres Formation
    Article Snippet: Then the assay was transformed into 384-well plate at the HTS Core at KU Lawrence, seeding 3000 cells/40 μL/well, with 0.35% DMSO as negative treatment control, and 3.5 mM sodium butyrate as positive control. .. The immunofluorescent assay was automated: Cell seeding, compound treatment, immunostaining, and image capture and analysis were performed with the WellMate® (Thermo Scientific Matrix), the ECHO 550® acoustic liquid handler (Labcyte), the ELx405® CWS Plate Washer (BioTek), the BD Pathway™ 855 High Content Imaging System (Becton, Dickinson), the Nikon Ti-inverted digital microscope (Nikon), and CellProfiler image-based data analysis software (Broad Institute).

    Immunostaining:

    Article Title: Targeting Epithelial-Mesenchymal Transition for Identification of Inhibitors for Pancreatic Cancer Cell Invasion and Tumor Spheres Formation
    Article Snippet: .. The immunofluorescent assay was automated: Cell seeding, compound treatment, immunostaining, and image capture and analysis were performed with the WellMate® (Thermo Scientific Matrix), the ECHO 550® acoustic liquid handler (Labcyte), the ELx405® CWS Plate Washer (BioTek), the BD Pathway™ 855 High Content Imaging System (Becton, Dickinson), the Nikon Ti-inverted digital microscope (Nikon), and CellProfiler image-based data analysis software (Broad Institute). .. Automated immunofluorescence staining started following 48 hours treatment by the compounds, and all wash steps were three gentle cycles of PBS addition by WellMate® and aspiration by ELx405®.

    Real-time Polymerase Chain Reaction:

    Article Title: Contrasting impacts of ocean acidification and warming on the molecular responses of CO2-resilient oysters
    Article Snippet: .. Three microliter qPCR reactions were prepared in duplicate in 384-well plates using an epMotion® 5075 pipetting robot (Eppendorf) and an Echo® 550 Liquid Handler (Labcyte). .. Each reaction contained 1.5 μl KAPA SYBR® FAST qPCR Master Mix (Kapa Biosystems), 300 nM each primer, 0.3 μl PCR grade water and 1 μl cDNA template (diluted 1:9). qPCR assays were carried out on a LightCycler® 480 II (Roche).

    Incubation:

    Article Title: From receptor binding kinetics to signal transduction; a missing link in predicting in vivo drug-action
    Article Snippet: The transfected cells were incubated for 24 h at 37 °C + 5% CO2 followed by treatment with Glo-substrate (3% v/v) for 2 h at room temperature. .. Subsequently, three different concentrations (0.07 nM, 0.21 nM and 0.7 nM) of Aprepitant or DFA were added to cells for 30 min (pre-incubation) using Echo™ 550 Liquid Handler (Labcyte), followed by addition of increasing concentrations of SP or NKA.

    Article Title: Screening-based approach to discover effective platinum-based chemotherapies for cancers with poor prognosis
    Article Snippet: PCL compounds were dispensed into 384-well plates using the Echo 550 acoustic liquid handler (Labcyte Inc. Sunnyvale, CA) before seeding the cells. .. Cell seeding (2000 cells/well for PANC-1 cells and 1200 cells/well for A549 cells), incubation (72 h for both cell lines) and cell viability determination were accomplished as described for the screening assays.

    Article Title: Quantitative modeling of transcription and translation of an all-E. coli cell-free system
    Article Snippet: .. TXTL reactions were assembled using a Labcyte Echo 550 Acoustic Liquid Handler, to volumes of 2 µl, and incubated at 29 °C. ..

    Article Title: POSTER SESSION ABSTRACTS
    Article Snippet: Using the Labcyte Echo 550 acoustic liquid handler and the Deerac Fluidics Equator HTS reagent dispenser, a collection of compounds was profiled by IC50 against the panel of metabolism assays in 1536-well format. .. The flexible incubation times of all assays, in addition to the single luminescent endpoint readout, allowed the assays to be run on the same plate and simplified data analysis.

    Article Title: Nanoscale Synaptic Membrane Mimetic Allows Unbiased High Throughput Screen That Targets Binding Sites for Alzheimer’s-Associated Aβ Oligomers
    Article Snippet: When applicable, NU2 antibody was applied using a Labcyte Echo 550 acoustic liquid handler before adding AβOs. .. The plate was incubated at room temperature on a plate shaker for 1 hour and AlphaLISA nickel-chelate acceptor beads were added to each well using the Labcyte Echo 550 resulting in a final concentration of 1 μg mL−1 .

    Article Title: In vitro implementation of robust gene regulation in a synthetic biomolecular integral controller
    Article Snippet: .. TXTL reactions were assembled using the Labcyte Echo 550 liquid handler, to volumes of 2 µl in a 96-well V-bottom plate (Corning Costar 3357 with caps Costar 3080) and incubated at 29 °C. .. DNA Plasmids were constructed using standard cloning techniques.

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: After incubated for 1 hr at room temperature, the AlphaScreen signals were read on EnSpire (PerkinElmer) in Alpha Mode. .. Briefly, different concentrations of FB23-2 were transferred to the 384-well plate by Echo® 550 liquid handler (Labcyte, USA), and then pre-incubated with BRD4(1,2) (BPS Biosciences) for 15 min at room temperature, which was followed by addition of the biotinylated H4 derived acetylated peptide to each well.

    Article Title: Identification and Characterization of Approved Drugs and Drug-Like Compounds as Covalent Escherichia coli ClpP Inhibitors
    Article Snippet: .. A549, HTC-116 and MCF-7 cells were seeded at 2000 cells/well (20 µL) in white, flat-bottom, sterile 384-well plates (#781073, Greiner Bio-One GmbH, Frickenhausen, Germany) and incubated for 24 h. A total of 200 nL ClpP inhibitors plus positive (valinomycin, 10 µM) and negative (DMSO, 1%) controls were transferred in the plate, using an Echo® 550 liquid handler (Labcyte) at 1:3 dilutions, starting from 200 μM, and incubated for an additional 48 h (95% air, 5% CO2 at 37 °C). .. After incubation, 20 µL of CellTiter-Glo® reagent were added per well and incubated at room temperature in the dark for another 30 min. Luminescence was quantified by an EnVision multi-plate reader (PerkinElmer, Rodgau, Germany).

    Cell Culture:

    Article Title: Marine Derived Hamacanthins as Lead for the Development of Novel PDGFR? Protein Kinase Inhibitors
    Article Snippet: Paragraph title: 3.3.2. Cell Culture and Proliferative Assays using HL-60, TK 10, 786-0, M 14, and MCF-7 Cells ... Compounds 5 and 8 that were dissolved in 100% DMSO (v/v) were added to test plates using the Echo 550® Liquid Handler (Labcyte Inc., Sunnyvale, UK).

    Expressing:

    Article Title: Targeting Epithelial-Mesenchymal Transition for Identification of Inhibitors for Pancreatic Cancer Cell Invasion and Tumor Spheres Formation
    Article Snippet: Paragraph title: Immunofluorescent assay for detection of E-cadherin expression ... The immunofluorescent assay was automated: Cell seeding, compound treatment, immunostaining, and image capture and analysis were performed with the WellMate® (Thermo Scientific Matrix), the ECHO 550® acoustic liquid handler (Labcyte), the ELx405® CWS Plate Washer (BioTek), the BD Pathway™ 855 High Content Imaging System (Becton, Dickinson), the Nikon Ti-inverted digital microscope (Nikon), and CellProfiler image-based data analysis software (Broad Institute).

    Article Title: Quantitative modeling of transcription and translation of an all-E. coli cell-free system
    Article Snippet: TXTL reactions were assembled using a Labcyte Echo 550 Acoustic Liquid Handler, to volumes of 2 µl, and incubated at 29 °C. .. Protein fluorescence kinetics measurements were performed with the reporter plasmid P70a-deGFP, expressing the truncated version of eGFP (25.4 kDA, 1 mg/mL = 39.38 µM) . deGFP fluorescence was measured on either a Biotek Neo2 or Biotek H1 plate reader at excitation and emission wavelengths of 485 nM and 528 nM, respectively, typically measuring every 3 minutes for 16 hours, with an incubation temperature of 29 °C.

    Article Title: In vitro implementation of robust gene regulation in a synthetic biomolecular integral controller
    Article Snippet: TXTL reactions The cell-free expression system used in this work is myTXTL from Arbor Biosciences. .. TXTL reactions were assembled using the Labcyte Echo 550 liquid handler, to volumes of 2 µl in a 96-well V-bottom plate (Corning Costar 3357 with caps Costar 3080) and incubated at 29 °C.

    Article Title: Contrasting impacts of ocean acidification and warming on the molecular responses of CO2-resilient oysters
    Article Snippet: qPCR analysis The transcriptional responses of B2 oysters to ocean acidification and warming were investigated by assessing the expression profiles of 60 genes involved in multiple biological processes. .. Three microliter qPCR reactions were prepared in duplicate in 384-well plates using an epMotion® 5075 pipetting robot (Eppendorf) and an Echo® 550 Liquid Handler (Labcyte).

    Modification:

    Article Title: Probing the Effects of Pyrimidine Functional Group Switches on Acyclic Fleximer Analogues for Antiviral Activity
    Article Snippet: Huh7 cells were seeded at 4 × 103 cells/well (384-well plates) in FluoroBrite Dulbecco’s modified Eagle’s medium (ThermoFisher, Waltham, MA, USA), supplemented with 10% (v /v ) fetal calf serum (Hyclone, GE Healthcare, Chicago, IL, USA) and 1x penicillin and streptomycin (ThermoFisher). .. The following day, compounds were added using an Echo 550 acoustic liquid handler (Labcyte, San Jose, CA, USA), and the plates were transferred to a BSL-4 laboratory.

    Transformation Assay:

    Article Title: Targeting Epithelial-Mesenchymal Transition for Identification of Inhibitors for Pancreatic Cancer Cell Invasion and Tumor Spheres Formation
    Article Snippet: Then the assay was transformed into 384-well plate at the HTS Core at KU Lawrence, seeding 3000 cells/40 μL/well, with 0.35% DMSO as negative treatment control, and 3.5 mM sodium butyrate as positive control. .. The immunofluorescent assay was automated: Cell seeding, compound treatment, immunostaining, and image capture and analysis were performed with the WellMate® (Thermo Scientific Matrix), the ECHO 550® acoustic liquid handler (Labcyte), the ELx405® CWS Plate Washer (BioTek), the BD Pathway™ 855 High Content Imaging System (Becton, Dickinson), the Nikon Ti-inverted digital microscope (Nikon), and CellProfiler image-based data analysis software (Broad Institute).

    Derivative Assay:

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: .. Briefly, different concentrations of FB23-2 were transferred to the 384-well plate by Echo® 550 liquid handler (Labcyte, USA), and then pre-incubated with BRD4(1,2) (BPS Biosciences) for 15 min at room temperature, which was followed by addition of the biotinylated H4 derived acetylated peptide to each well. .. Then the mixture of anti GST-Donor Antibody and Streptavidin-Acceptor were added to each well, and the assay plate was covered with a sealer to be incubated at room temperature for 1 hr.

    Transfection:

    Article Title: From receptor binding kinetics to signal transduction; a missing link in predicting in vivo drug-action
    Article Snippet: The transfected cells were incubated for 24 h at 37 °C + 5% CO2 followed by treatment with Glo-substrate (3% v/v) for 2 h at room temperature. .. Subsequently, three different concentrations (0.07 nM, 0.21 nM and 0.7 nM) of Aprepitant or DFA were added to cells for 30 min (pre-incubation) using Echo™ 550 Liquid Handler (Labcyte), followed by addition of increasing concentrations of SP or NKA.

    Infection:

    Article Title: Probing the Effects of Pyrimidine Functional Group Switches on Acyclic Fleximer Analogues for Antiviral Activity
    Article Snippet: The following day, compounds were added using an Echo 550 acoustic liquid handler (Labcyte, San Jose, CA, USA), and the plates were transferred to a BSL-4 laboratory. .. Cells were infected with EBOV-ZsGreen reporter virus [ , ] 2–3 h after the addition of compounds.

    Generated:

    Article Title: Contrasting impacts of ocean acidification and warming on the molecular responses of CO2-resilient oysters
    Article Snippet: Three microliter qPCR reactions were prepared in duplicate in 384-well plates using an epMotion® 5075 pipetting robot (Eppendorf) and an Echo® 550 Liquid Handler (Labcyte). .. Reaction efficiencies were calculated from standard curves generated in triplicate for each primer pair using five four-fold serial dilutions of a pool of cDNA samples (Additional file : Table S1).

    Inhibition:

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: Paragraph title: Inhibition of FTO inhibitors on epigenetic enzymes in vitro ... Briefly, different concentrations of FB23-2 were transferred to the 384-well plate by Echo® 550 liquid handler (Labcyte, USA), and then pre-incubated with BRD4(1,2) (BPS Biosciences) for 15 min at room temperature, which was followed by addition of the biotinylated H4 derived acetylated peptide to each well.

    Imaging:

    Article Title: Targeting Epithelial-Mesenchymal Transition for Identification of Inhibitors for Pancreatic Cancer Cell Invasion and Tumor Spheres Formation
    Article Snippet: .. The immunofluorescent assay was automated: Cell seeding, compound treatment, immunostaining, and image capture and analysis were performed with the WellMate® (Thermo Scientific Matrix), the ECHO 550® acoustic liquid handler (Labcyte), the ELx405® CWS Plate Washer (BioTek), the BD Pathway™ 855 High Content Imaging System (Becton, Dickinson), the Nikon Ti-inverted digital microscope (Nikon), and CellProfiler image-based data analysis software (Broad Institute). .. Automated immunofluorescence staining started following 48 hours treatment by the compounds, and all wash steps were three gentle cycles of PBS addition by WellMate® and aspiration by ELx405®.

    Sequencing:

    Article Title: POSTER SESSION ABSTRACTS
    Article Snippet: The Apollo 100 reduces the amounts of sequencing reagents necessary for running efficient reactions and removes most of the manual steps, allowing the user to place samples and reagents on the instrument, walk away, and return to sequencing products that are ready to inject onto a capillary sequencer. .. Using the Labcyte Echo 550 acoustic liquid handler and the Deerac Fluidics Equator HTS reagent dispenser, a collection of compounds was profiled by IC50 against the panel of metabolism assays in 1536-well format.

    Binding Assay:

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: To determine IC50 value for BRD4(1,2) inhibition, the HTRF-based biochemical binding assay was performed. .. Briefly, different concentrations of FB23-2 were transferred to the 384-well plate by Echo® 550 liquid handler (Labcyte, USA), and then pre-incubated with BRD4(1,2) (BPS Biosciences) for 15 min at room temperature, which was followed by addition of the biotinylated H4 derived acetylated peptide to each well.

    Reporter Gene Assay:

    Article Title: Identification of Adjuvantic Activity of Amphotericin B in a Novel, Multiplexed, Poly-TLR/NLR High-Throughput Screen
    Article Snippet: Compound transfers from source (80 nL of 10 mM stocks) to assay plates were performed using an Echo 550 acoustic liquid handler (Labcyte, Sunnyvale, CA). .. For most libraries, a target final concentration of 10 μM of compound (in a final volume of 80 μL for the multiplexed reporter gene-based assay described below) was achieved; the FDA Repurposed Library compounds were plated to obtain final concentrations of 2.5 μM.

    Immunofluorescence:

    Article Title: Targeting Epithelial-Mesenchymal Transition for Identification of Inhibitors for Pancreatic Cancer Cell Invasion and Tumor Spheres Formation
    Article Snippet: The immunofluorescent assay was automated: Cell seeding, compound treatment, immunostaining, and image capture and analysis were performed with the WellMate® (Thermo Scientific Matrix), the ECHO 550® acoustic liquid handler (Labcyte), the ELx405® CWS Plate Washer (BioTek), the BD Pathway™ 855 High Content Imaging System (Becton, Dickinson), the Nikon Ti-inverted digital microscope (Nikon), and CellProfiler image-based data analysis software (Broad Institute). .. Automated immunofluorescence staining started following 48 hours treatment by the compounds, and all wash steps were three gentle cycles of PBS addition by WellMate® and aspiration by ELx405®.

    Amplified Luminescent Proximity Homogenous Assay:

    Article Title: Nanoscale Synaptic Membrane Mimetic Allows Unbiased High Throughput Screen That Targets Binding Sites for Alzheimer’s-Associated Aβ Oligomers
    Article Snippet: Paragraph title: AlphaScreen assays ... When applicable, NU2 antibody was applied using a Labcyte Echo 550 acoustic liquid handler before adding AβOs.

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: After incubated for 1 hr at room temperature, the AlphaScreen signals were read on EnSpire (PerkinElmer) in Alpha Mode. .. Briefly, different concentrations of FB23-2 were transferred to the 384-well plate by Echo® 550 liquid handler (Labcyte, USA), and then pre-incubated with BRD4(1,2) (BPS Biosciences) for 15 min at room temperature, which was followed by addition of the biotinylated H4 derived acetylated peptide to each well.

    Fluorescence:

    Article Title: Quantitative modeling of transcription and translation of an all-E. coli cell-free system
    Article Snippet: TXTL reactions were assembled using a Labcyte Echo 550 Acoustic Liquid Handler, to volumes of 2 µl, and incubated at 29 °C. .. Protein fluorescence kinetics measurements were performed with the reporter plasmid P70a-deGFP, expressing the truncated version of eGFP (25.4 kDA, 1 mg/mL = 39.38 µM) . deGFP fluorescence was measured on either a Biotek Neo2 or Biotek H1 plate reader at excitation and emission wavelengths of 485 nM and 528 nM, respectively, typically measuring every 3 minutes for 16 hours, with an incubation temperature of 29 °C.

    Article Title: Probing the Effects of Pyrimidine Functional Group Switches on Acyclic Fleximer Analogues for Antiviral Activity
    Article Snippet: The following day, compounds were added using an Echo 550 acoustic liquid handler (Labcyte, San Jose, CA, USA), and the plates were transferred to a BSL-4 laboratory. .. ZsGreen fluorescence was determined 72 h post-infection using a Synergy H1MD plate reader (BioTek, Winooski, VT, USA).

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: Briefly, different concentrations of FB23-2 were transferred to the 384-well plate by Echo® 550 liquid handler (Labcyte, USA), and then pre-incubated with BRD4(1,2) (BPS Biosciences) for 15 min at room temperature, which was followed by addition of the biotinylated H4 derived acetylated peptide to each well. .. Read fluorescence emission at wavelengths of 665 nm and 620 nm on EnVision (PerkinElmer).

    Article Title: Contrasting impacts of ocean acidification and warming on the molecular responses of CO2-resilient oysters
    Article Snippet: Three microliter qPCR reactions were prepared in duplicate in 384-well plates using an epMotion® 5075 pipetting robot (Eppendorf) and an Echo® 550 Liquid Handler (Labcyte). .. The cycling program used consisted of 3 min at 95 °C followed by 45 cycles of 95 °C for 10 s, 60 °C for 20 s and 72 °C for 6 s. At the end of the qPCR cycles, melting curve analysis was performed by collecting fluorescence data between 65 and 95 °C at 0.5 °C increments.

    Microscopy:

    Article Title: Targeting Epithelial-Mesenchymal Transition for Identification of Inhibitors for Pancreatic Cancer Cell Invasion and Tumor Spheres Formation
    Article Snippet: .. The immunofluorescent assay was automated: Cell seeding, compound treatment, immunostaining, and image capture and analysis were performed with the WellMate® (Thermo Scientific Matrix), the ECHO 550® acoustic liquid handler (Labcyte), the ELx405® CWS Plate Washer (BioTek), the BD Pathway™ 855 High Content Imaging System (Becton, Dickinson), the Nikon Ti-inverted digital microscope (Nikon), and CellProfiler image-based data analysis software (Broad Institute). .. Automated immunofluorescence staining started following 48 hours treatment by the compounds, and all wash steps were three gentle cycles of PBS addition by WellMate® and aspiration by ELx405®.

    Polymerase Chain Reaction:

    Article Title: Contrasting impacts of ocean acidification and warming on the molecular responses of CO2-resilient oysters
    Article Snippet: Primers for quantitative (q) PCR analysis were designed to amplify the contigs most affected by ocean acidification (highest up- or down-regulation) (Additional file : Table S1). .. Three microliter qPCR reactions were prepared in duplicate in 384-well plates using an epMotion® 5075 pipetting robot (Eppendorf) and an Echo® 550 Liquid Handler (Labcyte).

    Staining:

    Article Title: Targeting Epithelial-Mesenchymal Transition for Identification of Inhibitors for Pancreatic Cancer Cell Invasion and Tumor Spheres Formation
    Article Snippet: The immunofluorescent assay was automated: Cell seeding, compound treatment, immunostaining, and image capture and analysis were performed with the WellMate® (Thermo Scientific Matrix), the ECHO 550® acoustic liquid handler (Labcyte), the ELx405® CWS Plate Washer (BioTek), the BD Pathway™ 855 High Content Imaging System (Becton, Dickinson), the Nikon Ti-inverted digital microscope (Nikon), and CellProfiler image-based data analysis software (Broad Institute). .. Automated immunofluorescence staining started following 48 hours treatment by the compounds, and all wash steps were three gentle cycles of PBS addition by WellMate® and aspiration by ELx405®.

    Viability Assay:

    Article Title: Marine Derived Hamacanthins as Lead for the Development of Novel PDGFR? Protein Kinase Inhibitors
    Article Snippet: Compounds 5 and 8 that were dissolved in 100% DMSO (v/v) were added to test plates using the Echo 550® Liquid Handler (Labcyte Inc., Sunnyvale, UK). .. The CellTiter-Glo® Viability Assay was used to determine the viability of cells using the standard protocol for this assay (Promega Corp., Madison, WI, USA).

    Plasmid Preparation:

    Article Title: From receptor binding kinetics to signal transduction; a missing link in predicting in vivo drug-action
    Article Snippet: The diluted plasmid solution was combined with the transfection reagent and incubated for 20 min at room temperature. .. Subsequently, three different concentrations (0.07 nM, 0.21 nM and 0.7 nM) of Aprepitant or DFA were added to cells for 30 min (pre-incubation) using Echo™ 550 Liquid Handler (Labcyte), followed by addition of increasing concentrations of SP or NKA.

    Article Title: Quantitative modeling of transcription and translation of an all-E. coli cell-free system
    Article Snippet: TXTL reactions were assembled using a Labcyte Echo 550 Acoustic Liquid Handler, to volumes of 2 µl, and incubated at 29 °C. .. Protein fluorescence kinetics measurements were performed with the reporter plasmid P70a-deGFP, expressing the truncated version of eGFP (25.4 kDA, 1 mg/mL = 39.38 µM) . deGFP fluorescence was measured on either a Biotek Neo2 or Biotek H1 plate reader at excitation and emission wavelengths of 485 nM and 528 nM, respectively, typically measuring every 3 minutes for 16 hours, with an incubation temperature of 29 °C.

    Software:

    Article Title: Targeting Epithelial-Mesenchymal Transition for Identification of Inhibitors for Pancreatic Cancer Cell Invasion and Tumor Spheres Formation
    Article Snippet: .. The immunofluorescent assay was automated: Cell seeding, compound treatment, immunostaining, and image capture and analysis were performed with the WellMate® (Thermo Scientific Matrix), the ECHO 550® acoustic liquid handler (Labcyte), the ELx405® CWS Plate Washer (BioTek), the BD Pathway™ 855 High Content Imaging System (Becton, Dickinson), the Nikon Ti-inverted digital microscope (Nikon), and CellProfiler image-based data analysis software (Broad Institute). .. Automated immunofluorescence staining started following 48 hours treatment by the compounds, and all wash steps were three gentle cycles of PBS addition by WellMate® and aspiration by ELx405®.

    In Vitro:

    Article Title: Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia
    Article Snippet: Paragraph title: Inhibition of FTO inhibitors on epigenetic enzymes in vitro ... Briefly, different concentrations of FB23-2 were transferred to the 384-well plate by Echo® 550 liquid handler (Labcyte, USA), and then pre-incubated with BRD4(1,2) (BPS Biosciences) for 15 min at room temperature, which was followed by addition of the biotinylated H4 derived acetylated peptide to each well.

    Concentration Assay:

    Article Title: Identification of Adjuvantic Activity of Amphotericin B in a Novel, Multiplexed, Poly-TLR/NLR High-Throughput Screen
    Article Snippet: Compound transfers from source (80 nL of 10 mM stocks) to assay plates were performed using an Echo 550 acoustic liquid handler (Labcyte, Sunnyvale, CA). .. For most libraries, a target final concentration of 10 μM of compound (in a final volume of 80 μL for the multiplexed reporter gene-based assay described below) was achieved; the FDA Repurposed Library compounds were plated to obtain final concentrations of 2.5 μM.

    Article Title: Screening-based approach to discover effective platinum-based chemotherapies for cancers with poor prognosis
    Article Snippet: Two-fold serial dilutions were performed for each drug alone and for their mixtures at a fixed concentration ratio (e.g. at (IC50 )Pt drug /(IC50 )PCL compd ratio) to yield 5 concentrations ranging from ¼ x IC50 to 4 x IC50 (see for the plate layout). .. PCL compounds were dispensed into 384-well plates using the Echo 550 acoustic liquid handler (Labcyte Inc. Sunnyvale, CA) before seeding the cells.

    Article Title: Nanoscale Synaptic Membrane Mimetic Allows Unbiased High Throughput Screen That Targets Binding Sites for Alzheimer’s-Associated Aβ Oligomers
    Article Snippet: When applicable, NU2 antibody was applied using a Labcyte Echo 550 acoustic liquid handler before adding AβOs. .. The plate was incubated at room temperature on a plate shaker for 1 hour and AlphaLISA nickel-chelate acceptor beads were added to each well using the Labcyte Echo 550 resulting in a final concentration of 1 μg mL−1 .

    Article Title: Marine Derived Hamacanthins as Lead for the Development of Novel PDGFR? Protein Kinase Inhibitors
    Article Snippet: Compounds 5 and 8 that were dissolved in 100% DMSO (v/v) were added to test plates using the Echo 550® Liquid Handler (Labcyte Inc., Sunnyvale, UK). .. The final DMSO concentration in the assay was 0.5% v/v.

    High Throughput Screening Assay:

    Article Title: Identification of Adjuvantic Activity of Amphotericin B in a Novel, Multiplexed, Poly-TLR/NLR High-Throughput Screen
    Article Snippet: Compounds for HTS screens Curated compound collections from the University of Kansas High Throughput Screening laboratory which include Life Chemicals (15,040), ChemBridge (43,736), ChemDiv (56,232), Selleck Bioactives (1649), TimTec (5000), and FDA Repurposed Library (2,286) were used. .. Compound transfers from source (80 nL of 10 mM stocks) to assay plates were performed using an Echo 550 acoustic liquid handler (Labcyte, Sunnyvale, CA).

    Direct Fluorescent Antibody Test:

    Article Title: From receptor binding kinetics to signal transduction; a missing link in predicting in vivo drug-action
    Article Snippet: .. Subsequently, three different concentrations (0.07 nM, 0.21 nM and 0.7 nM) of Aprepitant or DFA were added to cells for 30 min (pre-incubation) using Echo™ 550 Liquid Handler (Labcyte), followed by addition of increasing concentrations of SP or NKA. .. Real-time changes in the level of cAMP were detected using an Envision HTS microplate reader 2103 (PerkinElmer).

    Cell Viability Assay:

    Article Title: Identification and Characterization of Approved Drugs and Drug-Like Compounds as Covalent Escherichia coli ClpP Inhibitors
    Article Snippet: Cell Viability Assays Potential cell toxicity of the tested compounds was evaluated by ATP quantification using the CellTiter-Glo® luminescent cell viability assay (Promega, Madison, WI, USA). .. A549, HTC-116 and MCF-7 cells were seeded at 2000 cells/well (20 µL) in white, flat-bottom, sterile 384-well plates (#781073, Greiner Bio-One GmbH, Frickenhausen, Germany) and incubated for 24 h. A total of 200 nL ClpP inhibitors plus positive (valinomycin, 10 µM) and negative (DMSO, 1%) controls were transferred in the plate, using an Echo® 550 liquid handler (Labcyte) at 1:3 dilutions, starting from 200 μM, and incubated for an additional 48 h (95% air, 5% CO2 at 37 °C).

    other:

    Article Title: Small Polar Hits against S. aureus: Screening, Initial Hit Optimization, and Metabolomic Studies
    Article Snippet: Compounds from the library were stamped into each well from column 1 to column 22 of 384-well plates with a Echo 550 Liquid Handler (Labcyte Inc.).

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    Labcyte echo 550 acoustic liquid handler
    Plan diagram of Eve's laboratory robotic system . Layout diagram of Eve's laboratory robotic system, comprising: [1] Labcyte <t>Echo</t> 550 acoustic liquid handler, [2] BMG Pherastar reader, [3] MDS ImageXpress Micro cellular imager, [4] BMG Polarstar reader, [5] Cytomat 2C435 incubator, [6] Cytomat 6003 dry store, [7] FluidX DC-96pro capper/recapper, [8] two Variomag teleshake plate shakers and two Metrologic Orbit 1D barcode readers, [9] Cytomat linear actuator track, [10] robot plinth holding two Mitsubishi robot arms; models RV-3SJB and RV-3SJ, [11] FluidX Xtr-96 tube rack 2D barcode scanner, [12] Agilent (Velocity 11) Bravo liquid handler, [13] Thermo Combi-nL multidrop, [14] two Thermo Combi multidrops, and [15] consumables stacks for microplates, tube racks and tips. There are also two computers controlling the robotics, plus a networked computer server which runs all the other code vital to Eve's function: the chemistry knowledge base, QSARs and hypothesis generation, experiment planning, results relational database, data analysis etc. (not shown).
    Echo 550 Acoustic Liquid Handler, supplied by Labcyte, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    echo 550 acoustic liquid handler - by Bioz Stars, 2020-02
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    Plan diagram of Eve's laboratory robotic system . Layout diagram of Eve's laboratory robotic system, comprising: [1] Labcyte Echo 550 acoustic liquid handler, [2] BMG Pherastar reader, [3] MDS ImageXpress Micro cellular imager, [4] BMG Polarstar reader, [5] Cytomat 2C435 incubator, [6] Cytomat 6003 dry store, [7] FluidX DC-96pro capper/recapper, [8] two Variomag teleshake plate shakers and two Metrologic Orbit 1D barcode readers, [9] Cytomat linear actuator track, [10] robot plinth holding two Mitsubishi robot arms; models RV-3SJB and RV-3SJ, [11] FluidX Xtr-96 tube rack 2D barcode scanner, [12] Agilent (Velocity 11) Bravo liquid handler, [13] Thermo Combi-nL multidrop, [14] two Thermo Combi multidrops, and [15] consumables stacks for microplates, tube racks and tips. There are also two computers controlling the robotics, plus a networked computer server which runs all the other code vital to Eve's function: the chemistry knowledge base, QSARs and hypothesis generation, experiment planning, results relational database, data analysis etc. (not shown).

    Journal: Automated Experimentation

    Article Title: Towards Robot Scientists for autonomous scientific discovery

    doi: 10.1186/1759-4499-2-1

    Figure Lengend Snippet: Plan diagram of Eve's laboratory robotic system . Layout diagram of Eve's laboratory robotic system, comprising: [1] Labcyte Echo 550 acoustic liquid handler, [2] BMG Pherastar reader, [3] MDS ImageXpress Micro cellular imager, [4] BMG Polarstar reader, [5] Cytomat 2C435 incubator, [6] Cytomat 6003 dry store, [7] FluidX DC-96pro capper/recapper, [8] two Variomag teleshake plate shakers and two Metrologic Orbit 1D barcode readers, [9] Cytomat linear actuator track, [10] robot plinth holding two Mitsubishi robot arms; models RV-3SJB and RV-3SJ, [11] FluidX Xtr-96 tube rack 2D barcode scanner, [12] Agilent (Velocity 11) Bravo liquid handler, [13] Thermo Combi-nL multidrop, [14] two Thermo Combi multidrops, and [15] consumables stacks for microplates, tube racks and tips. There are also two computers controlling the robotics, plus a networked computer server which runs all the other code vital to Eve's function: the chemistry knowledge base, QSARs and hypothesis generation, experiment planning, results relational database, data analysis etc. (not shown).

    Article Snippet: Echo 550 acoustic liquid handler, Labcyte, Sunnyvale, CA, USA 8.

    Techniques:

    Representative result of high-throughput screening of BSH inhibitors. (A) Plate layout for screening BSH inhibitors. Pink boxes (columns 3–22) indicate test wells that contain library compounds of interest, BSH, and reaction mix containing substrate. Library compounds were shot into the well bottom using Echo 550/555 and enzyme and reaction mix were added using Multidrop Combi. Controls were added manually to the side wells (columns 1–2 and 23–24): blue boxes indicate activity controls (BSH, reaction mix containing substrate, and solvent DMSO), yellow boxes correspond to inhibition controls (BSH, reaction mix containing substrate, and NaIO 3 ), and white boxes are negative controls with no BSH added but include reaction mix as well as substrate. (B) The HTS results represented by one 384-well plate. Control wells indicate the assay proceeded normally. The wells in columns 3–22 that appeared clear, regardless of alternative color due to compound, and had low absorbance readings were considered hits (putative BSH inhibitors).

    Journal: PLoS ONE

    Article Title: Discovery of Bile Salt Hydrolase Inhibitors Using an Efficient High-Throughput Screening System

    doi: 10.1371/journal.pone.0085344

    Figure Lengend Snippet: Representative result of high-throughput screening of BSH inhibitors. (A) Plate layout for screening BSH inhibitors. Pink boxes (columns 3–22) indicate test wells that contain library compounds of interest, BSH, and reaction mix containing substrate. Library compounds were shot into the well bottom using Echo 550/555 and enzyme and reaction mix were added using Multidrop Combi. Controls were added manually to the side wells (columns 1–2 and 23–24): blue boxes indicate activity controls (BSH, reaction mix containing substrate, and solvent DMSO), yellow boxes correspond to inhibition controls (BSH, reaction mix containing substrate, and NaIO 3 ), and white boxes are negative controls with no BSH added but include reaction mix as well as substrate. (B) The HTS results represented by one 384-well plate. Control wells indicate the assay proceeded normally. The wells in columns 3–22 that appeared clear, regardless of alternative color due to compound, and had low absorbance readings were considered hits (putative BSH inhibitors).

    Article Snippet: Briefly, 0.25 µl of a specific compound from the Spectrum Collection library was transferred into the bottom of a clear 384-well microplate (Greiner cat # 781182) with flat bottom by using the Echo 550/555 Liquid Handlers (Labcyte).

    Techniques: High Throughput Screening Assay, Activity Assay, Inhibition