echo 550  (Labcyte)


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    Name:
    Echo 550 Liquid Handler
    Description:
    With the patented Dynamic Fluid Analysis technology the Echo 550 eliminates the need for calibration to run an assay These models enable high speed contamination free transfer of cDNA siRNA vectors proteins and crystallography reagents in nanoliter increments
    Catalog Number:
    001-2000
    Price:
    None
    Category:
    Liquid handler
    Score:
    85
    Quantity:
    1
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    Structured Review

    Labcyte echo 550
    A grid adaptor for liquid-handling robots. ( a ) A grid adaptor with a standard microplate footprint allows grids to be positioned in the plate position of liquid-handling robots, including the ARI Gryphon and the Labcyte Echo 550 liquid handler. A neoprene-lined torsion clip (black) grips the magnetic base and holds the grid in position. ( b ) The grid is indexed against two metal surfaces that protrude from the adaptor to ensure that the ports are correctly positioned. ( c ) A photograph of the grid adaptor holding a grid in the destination-plate position of a Labcyte Echo 550 liquid-handling robot.
    With the patented Dynamic Fluid Analysis technology the Echo 550 eliminates the need for calibration to run an assay These models enable high speed contamination free transfer of cDNA siRNA vectors proteins and crystallography reagents in nanoliter increments
    https://www.bioz.com/result/echo 550/product/Labcyte
    Average 99 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    echo 550 - by Bioz Stars, 2019-10
    99/100 stars

    Images

    1) Product Images from "High-density grids for efficient data collection from multiple crystals"

    Article Title: High-density grids for efficient data collection from multiple crystals

    Journal: Acta Crystallographica. Section D, Structural Biology

    doi: 10.1107/S2059798315020847

    A grid adaptor for liquid-handling robots. ( a ) A grid adaptor with a standard microplate footprint allows grids to be positioned in the plate position of liquid-handling robots, including the ARI Gryphon and the Labcyte Echo 550 liquid handler. A neoprene-lined torsion clip (black) grips the magnetic base and holds the grid in position. ( b ) The grid is indexed against two metal surfaces that protrude from the adaptor to ensure that the ports are correctly positioned. ( c ) A photograph of the grid adaptor holding a grid in the destination-plate position of a Labcyte Echo 550 liquid-handling robot.
    Figure Legend Snippet: A grid adaptor for liquid-handling robots. ( a ) A grid adaptor with a standard microplate footprint allows grids to be positioned in the plate position of liquid-handling robots, including the ARI Gryphon and the Labcyte Echo 550 liquid handler. A neoprene-lined torsion clip (black) grips the magnetic base and holds the grid in position. ( b ) The grid is indexed against two metal surfaces that protrude from the adaptor to ensure that the ports are correctly positioned. ( c ) A photograph of the grid adaptor holding a grid in the destination-plate position of a Labcyte Echo 550 liquid-handling robot.

    Techniques Used: Cross-linking Immunoprecipitation

    ( a ) An edge-on view of a grid with polycarbonate backing during data collection at LCLS-XPP. An Echo 550 liquid-handling robot was used to position droplets of a perforin crystal suspension inline with grid ports immediately prior to flash-cooling in liquid nitrogen. ( b ) Two crystals of perforin positioned over a grid port during data collection at LCLS-XPP. A hole is clearly visible in the top crystal where it has been exposed to the X-ray beam. The bottom crystal is still intact.
    Figure Legend Snippet: ( a ) An edge-on view of a grid with polycarbonate backing during data collection at LCLS-XPP. An Echo 550 liquid-handling robot was used to position droplets of a perforin crystal suspension inline with grid ports immediately prior to flash-cooling in liquid nitrogen. ( b ) Two crystals of perforin positioned over a grid port during data collection at LCLS-XPP. A hole is clearly visible in the top crystal where it has been exposed to the X-ray beam. The bottom crystal is still intact.

    Techniques Used:

    2) Product Images from "A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids"

    Article Title: A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids

    Journal: Acta Crystallographica. Section F, Structural Biology Communications

    doi: 10.1107/S2053230X14025126

    Plate lids result in better and more reproducible crystals. The figure shows crystals that were grown using 2.5 nl precipitant solution and 2.5 nl lysozyme ( a ) and trypsin ( b ) solution in CrystalQuick plates (approximately 2½ min for the Echo 550 to prepare each plate). Each type of protein was prepared on a plate that had a lid and on a plate that did not have a plate lid. On average, using a plate lid resulted in fewer but larger crystals of lysozyme and trypsin (left). The number of thaumatin crystals was approximately constant. X-ray data were obtained from one of the lysozyme crystals in each well in the middle row of the plate (row  D ). The data from crystals prepared using lids were merged and compared with the data from crystals prepared without lids. We also used the technique described in § 2.1 to measure the evaporation rate for each of the three types of mother liquor with no plate lid, and compared this with the deduced evaporation rate with a plate lid (right). Using lids during the 2½ min required for the Echo 550 to set up the 5 nl crystallization droplets allowed better, more reproducible and better diffracting crystals to be obtained.
    Figure Legend Snippet: Plate lids result in better and more reproducible crystals. The figure shows crystals that were grown using 2.5 nl precipitant solution and 2.5 nl lysozyme ( a ) and trypsin ( b ) solution in CrystalQuick plates (approximately 2½ min for the Echo 550 to prepare each plate). Each type of protein was prepared on a plate that had a lid and on a plate that did not have a plate lid. On average, using a plate lid resulted in fewer but larger crystals of lysozyme and trypsin (left). The number of thaumatin crystals was approximately constant. X-ray data were obtained from one of the lysozyme crystals in each well in the middle row of the plate (row D ). The data from crystals prepared using lids were merged and compared with the data from crystals prepared without lids. We also used the technique described in § 2.1 to measure the evaporation rate for each of the three types of mother liquor with no plate lid, and compared this with the deduced evaporation rate with a plate lid (right). Using lids during the 2½ min required for the Echo 550 to set up the 5 nl crystallization droplets allowed better, more reproducible and better diffracting crystals to be obtained.

    Techniques Used: Evaporation, Crystallization Assay

    3) Product Images from "A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids"

    Article Title: A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids

    Journal: Acta Crystallographica. Section F, Structural Biology Communications

    doi: 10.1107/S2053230X14025126

    Plate lids result in better and more reproducible crystals. The figure shows crystals that were grown using 2.5 nl precipitant solution and 2.5 nl lysozyme ( a ) and trypsin ( b ) solution in CrystalQuick plates (approximately 2½ min for the Echo 550 to prepare each plate). Each type of protein was prepared on a plate that had a lid and on a plate that did not have a plate lid. On average, using a plate lid resulted in fewer but larger crystals of lysozyme and trypsin (left). The number of thaumatin crystals was approximately constant. X-ray data were obtained from one of the lysozyme crystals in each well in the middle row of the plate (row  D ). The data from crystals prepared using lids were merged and compared with the data from crystals prepared without lids. We also used the technique described in § 2.1 to measure the evaporation rate for each of the three types of mother liquor with no plate lid, and compared this with the deduced evaporation rate with a plate lid (right). Using lids during the 2½ min required for the Echo 550 to set up the 5 nl crystallization droplets allowed better, more reproducible and better diffracting crystals to be obtained.
    Figure Legend Snippet: Plate lids result in better and more reproducible crystals. The figure shows crystals that were grown using 2.5 nl precipitant solution and 2.5 nl lysozyme ( a ) and trypsin ( b ) solution in CrystalQuick plates (approximately 2½ min for the Echo 550 to prepare each plate). Each type of protein was prepared on a plate that had a lid and on a plate that did not have a plate lid. On average, using a plate lid resulted in fewer but larger crystals of lysozyme and trypsin (left). The number of thaumatin crystals was approximately constant. X-ray data were obtained from one of the lysozyme crystals in each well in the middle row of the plate (row D ). The data from crystals prepared using lids were merged and compared with the data from crystals prepared without lids. We also used the technique described in § 2.1 to measure the evaporation rate for each of the three types of mother liquor with no plate lid, and compared this with the deduced evaporation rate with a plate lid (right). Using lids during the 2½ min required for the Echo 550 to set up the 5 nl crystallization droplets allowed better, more reproducible and better diffracting crystals to be obtained.

    Techniques Used: Evaporation, Crystallization Assay

    4) Product Images from "A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids"

    Article Title: A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids

    Journal: Acta Crystallographica. Section F, Structural Biology Communications

    doi: 10.1107/S2053230X14025126

    Plate lids result in better and more reproducible crystals. The figure shows crystals that were grown using 2.5 nl precipitant solution and 2.5 nl lysozyme ( a ) and trypsin ( b ) solution in CrystalQuick plates (approximately 2½ min for the Echo 550 to prepare each plate). Each type of protein was prepared on a plate that had a lid and on a plate that did not have a plate lid. On average, using a plate lid resulted in fewer but larger crystals of lysozyme and trypsin (left). The number of thaumatin crystals was approximately constant. X-ray data were obtained from one of the lysozyme crystals in each well in the middle row of the plate (row  D ). The data from crystals prepared using lids were merged and compared with the data from crystals prepared without lids. We also used the technique described in § 2.1 to measure the evaporation rate for each of the three types of mother liquor with no plate lid, and compared this with the deduced evaporation rate with a plate lid (right). Using lids during the 2½ min required for the Echo 550 to set up the 5 nl crystallization droplets allowed better, more reproducible and better diffracting crystals to be obtained.
    Figure Legend Snippet: Plate lids result in better and more reproducible crystals. The figure shows crystals that were grown using 2.5 nl precipitant solution and 2.5 nl lysozyme ( a ) and trypsin ( b ) solution in CrystalQuick plates (approximately 2½ min for the Echo 550 to prepare each plate). Each type of protein was prepared on a plate that had a lid and on a plate that did not have a plate lid. On average, using a plate lid resulted in fewer but larger crystals of lysozyme and trypsin (left). The number of thaumatin crystals was approximately constant. X-ray data were obtained from one of the lysozyme crystals in each well in the middle row of the plate (row D ). The data from crystals prepared using lids were merged and compared with the data from crystals prepared without lids. We also used the technique described in § 2.1 to measure the evaporation rate for each of the three types of mother liquor with no plate lid, and compared this with the deduced evaporation rate with a plate lid (right). Using lids during the 2½ min required for the Echo 550 to set up the 5 nl crystallization droplets allowed better, more reproducible and better diffracting crystals to be obtained.

    Techniques Used: Evaporation, Crystallization Assay

    5) Product Images from "A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids"

    Article Title: A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids

    Journal: Acta Crystallographica. Section F, Structural Biology Communications

    doi: 10.1107/S2053230X14025126

    Plate lids result in better and more reproducible crystals. The figure shows crystals that were grown using 2.5 nl precipitant solution and 2.5 nl lysozyme ( a ) and trypsin ( b ) solution in CrystalQuick plates (approximately 2½ min for the Echo 550 to prepare each plate). Each type of protein was prepared on a plate that had a lid and on a plate that did not have a plate lid. On average, using a plate lid resulted in fewer but larger crystals of lysozyme and trypsin (left). The number of thaumatin crystals was approximately constant. X-ray data were obtained from one of the lysozyme crystals in each well in the middle row of the plate (row  D ). The data from crystals prepared using lids were merged and compared with the data from crystals prepared without lids. We also used the technique described in § 2.1 to measure the evaporation rate for each of the three types of mother liquor with no plate lid, and compared this with the deduced evaporation rate with a plate lid (right). Using lids during the 2½ min required for the Echo 550 to set up the 5 nl crystallization droplets allowed better, more reproducible and better diffracting crystals to be obtained.
    Figure Legend Snippet: Plate lids result in better and more reproducible crystals. The figure shows crystals that were grown using 2.5 nl precipitant solution and 2.5 nl lysozyme ( a ) and trypsin ( b ) solution in CrystalQuick plates (approximately 2½ min for the Echo 550 to prepare each plate). Each type of protein was prepared on a plate that had a lid and on a plate that did not have a plate lid. On average, using a plate lid resulted in fewer but larger crystals of lysozyme and trypsin (left). The number of thaumatin crystals was approximately constant. X-ray data were obtained from one of the lysozyme crystals in each well in the middle row of the plate (row D ). The data from crystals prepared using lids were merged and compared with the data from crystals prepared without lids. We also used the technique described in § 2.1 to measure the evaporation rate for each of the three types of mother liquor with no plate lid, and compared this with the deduced evaporation rate with a plate lid (right). Using lids during the 2½ min required for the Echo 550 to set up the 5 nl crystallization droplets allowed better, more reproducible and better diffracting crystals to be obtained.

    Techniques Used: Evaporation, Crystallization Assay

    6) Product Images from "A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids"

    Article Title: A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids

    Journal: Acta Crystallographica. Section F, Structural Biology Communications

    doi: 10.1107/S2053230X14025126

    Plate lids result in better and more reproducible crystals. The figure shows crystals that were grown using 2.5 nl precipitant solution and 2.5 nl lysozyme ( a ) and trypsin ( b ) solution in CrystalQuick plates (approximately 2½ min for the Echo 550 to prepare each plate). Each type of protein was prepared on a plate that had a lid and on a plate that did not have a plate lid. On average, using a plate lid resulted in fewer but larger crystals of lysozyme and trypsin (left). The number of thaumatin crystals was approximately constant. X-ray data were obtained from one of the lysozyme crystals in each well in the middle row of the plate (row  D ). The data from crystals prepared using lids were merged and compared with the data from crystals prepared without lids. We also used the technique described in § 2.1 to measure the evaporation rate for each of the three types of mother liquor with no plate lid, and compared this with the deduced evaporation rate with a plate lid (right). Using lids during the 2½ min required for the Echo 550 to set up the 5 nl crystallization droplets allowed better, more reproducible and better diffracting crystals to be obtained.
    Figure Legend Snippet: Plate lids result in better and more reproducible crystals. The figure shows crystals that were grown using 2.5 nl precipitant solution and 2.5 nl lysozyme ( a ) and trypsin ( b ) solution in CrystalQuick plates (approximately 2½ min for the Echo 550 to prepare each plate). Each type of protein was prepared on a plate that had a lid and on a plate that did not have a plate lid. On average, using a plate lid resulted in fewer but larger crystals of lysozyme and trypsin (left). The number of thaumatin crystals was approximately constant. X-ray data were obtained from one of the lysozyme crystals in each well in the middle row of the plate (row D ). The data from crystals prepared using lids were merged and compared with the data from crystals prepared without lids. We also used the technique described in § 2.1 to measure the evaporation rate for each of the three types of mother liquor with no plate lid, and compared this with the deduced evaporation rate with a plate lid (right). Using lids during the 2½ min required for the Echo 550 to set up the 5 nl crystallization droplets allowed better, more reproducible and better diffracting crystals to be obtained.

    Techniques Used: Evaporation, Crystallization Assay

    Related Articles

    Amplified Luminescent Proximity Homogenous Assay:

    Article Title: 8-Substituted Pyrido[3,4-d]pyrimidin-4(3H)-one Derivatives As Potent, Cell Permeable, KDM4 (JMJD2) and KDM5 (JARID1) Histone Lysine Demethylase Inhibitors
    Article Snippet: Paragraph title: KDM4A and KDM4B AlphaScreen Biochemical Assays ... Compounds were dispensed using an ECHO 550 acoustic dispenser (Labcyte Inc., Sunnyvale, CA, USA) to generate 8 pt dilution curves directly into 384-well Proxiplates (no. 6008289, PerkinElmer, Waltham, MA, USA) to give final assay concentrations in the range 0.0005–100 μM in 2% (v/v) DMSO as appropriate.

    Article Title: A cellular chemical probe targeting the chromodomains of Polycomb Repressive Complex 1
    Article Snippet: The temperature was raised with a step of 3 °C per minute from 25 °C to 96 °C and fluorescence readings were taken at each interval. .. The demethylase AlphaScreen® assay was performed in 384-well plate format using white proxiplates (Perkin Elmer) and compound transfers (100 nl) were performed using an ECHO 550 Acoustic Dispenser (Labcyte). .. All subsequent steps were carried out in assay buffer (50 mM HEPES pH 7.5, 0.1% (w/v) Bovine Serum Albumin and 0.01 % (v/v) Tween-20).

    Article Title: Assessing histone demethylase inhibitors in cells: lessons learned
    Article Snippet: Paragraph title: AlphaScreen ... Compounds (100 nL) were dispensed with an ECHO® 550 acoustic dispenser (Labcyte Inc™, Sunnyvale, CA, USA) to generate 10–12 pt dilution curves directly into 384-well Proxiplates (#6008289, PerkinElmer, Waltham, MA, USA) to give final assay concentrations in the range 0.00015–30 µM in 2%(v/v) DMSO where appropriate.

    Luciferase:

    Article Title: Cell-based high-throughput compound screening reveals functional interaction between oncofetal HMGA2 and topoisomerase I
    Article Snippet: After 24 h, cells from identical co-transfections were pooled, and about 10 000 cells were plated per well on a 384-well white plate with clear-bottom (Greiner bio one) using the LABCYTE Echo 550 liquid handler. .. After 24 h, cells from identical co-transfections were pooled, and about 10 000 cells were plated per well on a 384-well white plate with clear-bottom (Greiner bio one) using the LABCYTE Echo 550 liquid handler.

    Filtration:

    Article Title: 8-Substituted Pyrido[3,4-d]pyrimidin-4(3H)-one Derivatives As Potent, Cell Permeable, KDM4 (JMJD2) and KDM5 (JARID1) Histone Lysine Demethylase Inhibitors
    Article Snippet: Following infection of Sf9 insect cells, protein was purified by GSH affinity chromatography followed by gel filtration (Superdex 200). .. Compounds were dispensed using an ECHO 550 acoustic dispenser (Labcyte Inc., Sunnyvale, CA, USA) to generate 8 pt dilution curves directly into 384-well Proxiplates (no. 6008289, PerkinElmer, Waltham, MA, USA) to give final assay concentrations in the range 0.0005–100 μM in 2% (v/v) DMSO as appropriate.

    Positive Control:

    Article Title: A Cell-Free Fluorometric High-Throughput Screen for Inhibitors of Rtt109-Catalyzed Histone Acetylation
    Article Snippet: Briefly, test compounds and the control inhibitor ATA were first added to the appropriate wells using an Echo 550 contactless liquid handler (Labcyte). .. The KAT reaction was then initiated by dispensing 10 µL acetyl-CoA to appropriate wells to yield a final reaction concentration of 10 µM acetyl-CoA and 15 µL total solution volume per well.

    Picogreen Assay:

    Article Title: Linking the Human Gut Microbiome to Inflammatory Cytokine Production Capacity
    Article Snippet: DNA samples were subsequently quantified by Quant-iT PicoGreen dsDNA Assay (Life Technologies) and normalized to a concentration of 50 pg/µL. .. Libraries were pooled by transferring equal volumes of each library using a Labcyte Echo 550 liquid handler.

    Construct:

    Article Title: A Chemical Probe Strategy for Interrogating Inhibitor Selectivity Across the MEK Kinase Family
    Article Snippet: Small molecule screening by fluorescence-based thermal shift (FTS) assay was performed as described previously using an Echo550 (Labcyte) for compound transfer and a Mantis (Formulatrix, Bedford, MA) to dispense protein into 384-well PCR plates. .. The PCR plates were sealed with an optical seal, shaken, and centrifuged after protein and compounds were added.

    Cytotoxicity Assay:

    Article Title: Iron chelators target both proliferating and quiescent cancer cells
    Article Snippet: The spheroid-based cytotoxicity assay was performed as described previously , with minor modifications. .. Then, drugs were administered using Echo Liquid Handler 550 (Labcyte, Sunnyvale, CA).

    Real-time Polymerase Chain Reaction:

    Article Title: A Chemical Probe Strategy for Interrogating Inhibitor Selectivity Across the MEK Kinase Family
    Article Snippet: Small molecule screening by fluorescence-based thermal shift (FTS) assay was performed as described previously using an Echo550 (Labcyte) for compound transfer and a Mantis (Formulatrix, Bedford, MA) to dispense protein into 384-well PCR plates. .. The PCR plates were sealed with an optical seal, shaken, and centrifuged after protein and compounds were added.

    Incubation:

    Article Title: Metformin increases glucose uptake and acts renoprotectively by reducing SHIP2 activity
    Article Snippet: Different volumes, resulting in desired final concentrations of inhibitors, were transferred to the wells of 384-well plates by using the Echo550 acoustic dispenser (Labcyte, San Jose, CA, USA). .. The phosphatase reaction was started by adding PI(3,4,5)P3 diluted in the reaction buffer (7.5 µl) to each well to reach a final concentration of 100 µM PI(3,4,5)P3 in the 15 µl reaction volume.

    Article Title: Marine Derived Hamacanthins as Lead for the Development of Novel PDGFR? Protein Kinase Inhibitors
    Article Snippet: All plates were incubated for 24 h at 37 °C in a humidified atmosphere with 5% CO2 . .. Compounds 5 and 8 that were dissolved in 100% DMSO (v/v) were added to test plates using the Echo 550® Liquid Handler (Labcyte Inc., Sunnyvale, UK).

    Activity Assay:

    Article Title: A virtual screen identified C96 as a novel inhibitor of phosphatidylinositol 3-kinase that displays potent preclinical activity against multiple myeloma in vitro and in vivo
    Article Snippet: Paragraph title: Kinase activity in cell-free assays ... Subsequently, 5 nL of serially diluted C96 (in pure DMSO) was delivered into diluted kinase and substrate mixture by using Echo 550 (LabCyte Inc. Sunnyvale, CA).

    Article Title: 8-Substituted Pyrido[3,4-d]pyrimidin-4(3H)-one Derivatives As Potent, Cell Permeable, KDM4 (JMJD2) and KDM5 (JARID1) Histone Lysine Demethylase Inhibitors
    Article Snippet: Enzyme activity was measured using an AlphaScreen assay that monitored the demethylation of a biotinylated trimethylated H3K9 peptide using a H3K9 dimethyl specific antibody and appropriate donor and acceptors beads from PerkinElmer Life Sciences. .. Compounds were dispensed using an ECHO 550 acoustic dispenser (Labcyte Inc., Sunnyvale, CA, USA) to generate 8 pt dilution curves directly into 384-well Proxiplates (no. 6008289, PerkinElmer, Waltham, MA, USA) to give final assay concentrations in the range 0.0005–100 μM in 2% (v/v) DMSO as appropriate.

    Article Title: Metformin increases glucose uptake and acts renoprotectively by reducing SHIP2 activity
    Article Snippet: Paragraph title: SHIP2 activity measurement ... Different volumes, resulting in desired final concentrations of inhibitors, were transferred to the wells of 384-well plates by using the Echo550 acoustic dispenser (Labcyte, San Jose, CA, USA).

    Cell Culture:

    Article Title: Marine Derived Hamacanthins as Lead for the Development of Novel PDGFR? Protein Kinase Inhibitors
    Article Snippet: Paragraph title: 3.3.2. Cell Culture and Proliferative Assays using HL-60, TK 10, 786-0, M 14, and MCF-7 Cells ... Compounds 5 and 8 that were dissolved in 100% DMSO (v/v) were added to test plates using the Echo 550® Liquid Handler (Labcyte Inc., Sunnyvale, UK).

    DNA Synthesis:

    Article Title: Smart DNA Fabrication Using Sound Waves
    Article Snippet: To date, acoustic dispensers have mainly been used in screening libraries of compounds. .. In this paper, we describe the first application of this powerful technology to the rapidly developing field of synthetic biology, for DNA synthesis and assembly at the nanoliter scale using a Labcyte Echo 550 acoustic dispenser. .. We were able to successfully downscale PCRs and the popular one-pot DNA assembly methods, Golden Gate and Gibson assemblies, from the microliter to the nanoliter scale with high assembly efficiency, which effectively cut the reagent cost by 20- to 100-fold.

    Crystallization Assay:

    Article Title: Macromolecular crystallography beamline X25 at the NSLS
    Article Snippet: A liquid-handling device is available at the NSLS for PXRR users trying to figure out new crystallization conditions. .. The Echo 550 (Labcyte, Sunnyvale, USA) is available to not only set up crystallization trays using very small volumes of protein (∼50 µL initial volume for 2000 conditions) but it can also be used to mount crystals onto meshes to bring to the beamline. .. Very small crystals of a few micrometers or less benefit from reduced background from 2.5 nL of mother liquor that is transferred with each crystal.

    Article Title: A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids
    Article Snippet: For each crystallization plate design, we used a simple strategy to calculate the rate of water loss that affected experiments with and without the plate lids under different environmental conditions and using different common precipitants (we also visually assessed the dehydration of each droplet). .. We then prepared crystallization microplates using 2.5 nl protein solution and 2.5 nl precipitant solution using an Echo 550 (Labcyte Inc., Sunnyvale, California, USA) acoustic droplet ejection (ADE) instrument. .. Plates that were covered with a plate lid during preparation were compared with plates that were open to room air while the Echo 550 was loading the plate.

    Transferring:

    Article Title: Linking the Human Gut Microbiome to Inflammatory Cytokine Production Capacity
    Article Snippet: Whole-genome shotgun sequencing libraries were prepared according to the manufacturer’s instructions using the Nextera XT DNA Library Preparation kit (Illumina) with 100–250 pg input DNA. .. Libraries were pooled by transferring equal volumes of each library using a Labcyte Echo 550 liquid handler. .. The concentrations and insert size ranges for each pooled library were checked using an Agilent Bioanalyzer DNA 1000 kit (Agilent Technologies).

    Infection:

    Article Title: 8-Substituted Pyrido[3,4-d]pyrimidin-4(3H)-one Derivatives As Potent, Cell Permeable, KDM4 (JMJD2) and KDM5 (JARID1) Histone Lysine Demethylase Inhibitors
    Article Snippet: Following infection of Sf9 insect cells, protein was purified by GSH affinity chromatography followed by gel filtration (Superdex 200). .. Compounds were dispensed using an ECHO 550 acoustic dispenser (Labcyte Inc., Sunnyvale, CA, USA) to generate 8 pt dilution curves directly into 384-well Proxiplates (no. 6008289, PerkinElmer, Waltham, MA, USA) to give final assay concentrations in the range 0.0005–100 μM in 2% (v/v) DMSO as appropriate.

    Generated:

    Article Title: 8-Substituted Pyrido[3,4-d]pyrimidin-4(3H)-one Derivatives As Potent, Cell Permeable, KDM4 (JMJD2) and KDM5 (JARID1) Histone Lysine Demethylase Inhibitors
    Article Snippet: Protein production and purification: Baculovirus containing residues 1–500 of KDM4B with an N-terminal GST tag were generated according to Bac-to-Bac protocol. .. Compounds were dispensed using an ECHO 550 acoustic dispenser (Labcyte Inc., Sunnyvale, CA, USA) to generate 8 pt dilution curves directly into 384-well Proxiplates (no. 6008289, PerkinElmer, Waltham, MA, USA) to give final assay concentrations in the range 0.0005–100 μM in 2% (v/v) DMSO as appropriate.

    other:

    Article Title: The Deconstructed Granuloma: A Complex High-Throughput Drug Screening Platform for the Discovery of Host-Directed Therapeutics Against Tuberculosis
    Article Snippet: Test drugs were arrayed from a 2 mM stock solution in DMSO via acoustic dispense (ECHO 550 liquid handler; Labcyte Inc.) into 384-well Axygen P-384-120SQ-C-S plates, sealed and stored frozen prior to use.

    Protein Concentration:

    Article Title: A Chemical Probe Strategy for Interrogating Inhibitor Selectivity Across the MEK Kinase Family
    Article Snippet: Small molecule screening by fluorescence-based thermal shift (FTS) assay was performed as described previously using an Echo550 (Labcyte) for compound transfer and a Mantis (Formulatrix, Bedford, MA) to dispense protein into 384-well PCR plates. .. Purified MAP2K4-EE was appropriately diluted in a buffer containing 100 mM Hepes at pH 7.5 and 150 mM NaCl.

    Article Title: A Fluorescence-Based Thermal Shift Assay Identifies Inhibitors of Mitogen Activated Protein Kinase Kinase 4
    Article Snippet: Small molecule screening by FTS assay was performed as described previously , but with modifications, using an Echo550 (Labcyte) for compound transfer and a Mosquito (TTP Labtech) to dispense protein into 384 well PCR plates. .. Purified MAP2K4-EE was appropriately diluted in a buffer containing 100 mM Hepes, pH 7.5, 150 mM NaCl.

    Polymerase Chain Reaction:

    Article Title: A Chemical Probe Strategy for Interrogating Inhibitor Selectivity Across the MEK Kinase Family
    Article Snippet: A Cobalt Affinity chromatography column was employed to purify constructs. .. Small molecule screening by fluorescence-based thermal shift (FTS) assay was performed as described previously using an Echo550 (Labcyte) for compound transfer and a Mantis (Formulatrix, Bedford, MA) to dispense protein into 384-well PCR plates. .. Purified MAP2K4-EE was appropriately diluted in a buffer containing 100 mM Hepes at pH 7.5 and 150 mM NaCl.

    Article Title: A Fluorescence-Based Thermal Shift Assay Identifies Inhibitors of Mitogen Activated Protein Kinase Kinase 4
    Article Snippet: Human MAP2K4-KD (80–399; ) was expressed and purified in identical fashion but with the gene of interest being cloned into the pMCSG28 vector with a C-terminal 6X histidine tag instead. .. Small molecule screening by FTS assay was performed as described previously , but with modifications, using an Echo550 (Labcyte) for compound transfer and a Mosquito (TTP Labtech) to dispense protein into 384 well PCR plates. .. Purified MAP2K4-EE was appropriately diluted in a buffer containing 100 mM Hepes, pH 7.5, 150 mM NaCl.

    Binding Assay:

    Article Title: A Chemical Probe Strategy for Interrogating Inhibitor Selectivity Across the MEK Kinase Family
    Article Snippet: Paragraph title: Secondary Binding Assay ... Small molecule screening by fluorescence-based thermal shift (FTS) assay was performed as described previously using an Echo550 (Labcyte) for compound transfer and a Mantis (Formulatrix, Bedford, MA) to dispense protein into 384-well PCR plates.

    DNA Extraction:

    Article Title: Linking the Human Gut Microbiome to Inflammatory Cytokine Production Capacity
    Article Snippet: Libraries were pooled by transferring equal volumes of each library using a Labcyte Echo 550 liquid handler. .. Libraries were pooled by transferring equal volumes of each library using a Labcyte Echo 550 liquid handler.

    Fluorescence:

    Article Title: A Chemical Probe Strategy for Interrogating Inhibitor Selectivity Across the MEK Kinase Family
    Article Snippet: A Cobalt Affinity chromatography column was employed to purify constructs. .. Small molecule screening by fluorescence-based thermal shift (FTS) assay was performed as described previously using an Echo550 (Labcyte) for compound transfer and a Mantis (Formulatrix, Bedford, MA) to dispense protein into 384-well PCR plates. .. Purified MAP2K4-EE was appropriately diluted in a buffer containing 100 mM Hepes at pH 7.5 and 150 mM NaCl.

    Article Title: A Fluorescence-Based Thermal Shift Assay Identifies Inhibitors of Mitogen Activated Protein Kinase Kinase 4
    Article Snippet: Paragraph title: Fluorescence-based thermal shift (FTS) assay ... Small molecule screening by FTS assay was performed as described previously , but with modifications, using an Echo550 (Labcyte) for compound transfer and a Mosquito (TTP Labtech) to dispense protein into 384 well PCR plates.

    Isolation:

    Article Title: Linking the Human Gut Microbiome to Inflammatory Cytokine Production Capacity
    Article Snippet: DNA was isolated with the AllPrep DNA/RNA Mini Kit (Qiagen) with the addition of mechanical lysis. .. Libraries were pooled by transferring equal volumes of each library using a Labcyte Echo 550 liquid handler.

    Purification:

    Article Title: A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids
    Article Snippet: We then prepared crystallization microplates using 2.5 nl protein solution and 2.5 nl precipitant solution using an Echo 550 (Labcyte Inc., Sunnyvale, California, USA) acoustic droplet ejection (ADE) instrument. .. We then prepared crystallization microplates using 2.5 nl protein solution and 2.5 nl precipitant solution using an Echo 550 (Labcyte Inc., Sunnyvale, California, USA) acoustic droplet ejection (ADE) instrument.

    Article Title: 8-Substituted Pyrido[3,4-d]pyrimidin-4(3H)-one Derivatives As Potent, Cell Permeable, KDM4 (JMJD2) and KDM5 (JARID1) Histone Lysine Demethylase Inhibitors
    Article Snippet: Purification of KDM4A is described in the crystallography section. .. Compounds were dispensed using an ECHO 550 acoustic dispenser (Labcyte Inc., Sunnyvale, CA, USA) to generate 8 pt dilution curves directly into 384-well Proxiplates (no. 6008289, PerkinElmer, Waltham, MA, USA) to give final assay concentrations in the range 0.0005–100 μM in 2% (v/v) DMSO as appropriate.

    Article Title: A Cell-Free Fluorometric High-Throughput Screen for Inhibitors of Rtt109-Catalyzed Histone Acetylation
    Article Snippet: Briefly, test compounds and the control inhibitor ATA were first added to the appropriate wells using an Echo 550 contactless liquid handler (Labcyte). .. Briefly, test compounds and the control inhibitor ATA were first added to the appropriate wells using an Echo 550 contactless liquid handler (Labcyte).

    Sequencing:

    Article Title: Linking the Human Gut Microbiome to Inflammatory Cytokine Production Capacity
    Article Snippet: Paragraph title: Stool Samples and Sequencing ... Libraries were pooled by transferring equal volumes of each library using a Labcyte Echo 550 liquid handler.

    Affinity Chromatography:

    Article Title: 8-Substituted Pyrido[3,4-d]pyrimidin-4(3H)-one Derivatives As Potent, Cell Permeable, KDM4 (JMJD2) and KDM5 (JARID1) Histone Lysine Demethylase Inhibitors
    Article Snippet: Following infection of Sf9 insect cells, protein was purified by GSH affinity chromatography followed by gel filtration (Superdex 200). .. Compounds were dispensed using an ECHO 550 acoustic dispenser (Labcyte Inc., Sunnyvale, CA, USA) to generate 8 pt dilution curves directly into 384-well Proxiplates (no. 6008289, PerkinElmer, Waltham, MA, USA) to give final assay concentrations in the range 0.0005–100 μM in 2% (v/v) DMSO as appropriate.

    Lysis:

    Article Title: Linking the Human Gut Microbiome to Inflammatory Cytokine Production Capacity
    Article Snippet: DNA was isolated with the AllPrep DNA/RNA Mini Kit (Qiagen) with the addition of mechanical lysis. .. Libraries were pooled by transferring equal volumes of each library using a Labcyte Echo 550 liquid handler.

    Shotgun Sequencing:

    Article Title: Linking the Human Gut Microbiome to Inflammatory Cytokine Production Capacity
    Article Snippet: Whole-genome shotgun sequencing libraries were prepared according to the manufacturer’s instructions using the Nextera XT DNA Library Preparation kit (Illumina) with 100–250 pg input DNA. .. Libraries were pooled by transferring equal volumes of each library using a Labcyte Echo 550 liquid handler.

    Viability Assay:

    Article Title: Iron chelators target both proliferating and quiescent cancer cells
    Article Snippet: Paragraph title: Spheroid viability assay ... Then, drugs were administered using Echo Liquid Handler 550 (Labcyte, Sunnyvale, CA).

    Article Title: Marine Derived Hamacanthins as Lead for the Development of Novel PDGFR? Protein Kinase Inhibitors
    Article Snippet: Compounds 5 and 8 that were dissolved in 100% DMSO (v/v) were added to test plates using the Echo 550® Liquid Handler (Labcyte Inc., Sunnyvale, UK). .. Compounds 5 and 8 that were dissolved in 100% DMSO (v/v) were added to test plates using the Echo 550® Liquid Handler (Labcyte Inc., Sunnyvale, UK).

    Software:

    Article Title: Macromolecular crystallography beamline X25 at the NSLS
    Article Snippet: An extensive set of crystallography software is available at these computers but these areas are mainly used to finish work and back up data after allocated beam time has ended. .. The Echo 550 (Labcyte, Sunnyvale, USA) is available to not only set up crystallization trays using very small volumes of protein (∼50 µL initial volume for 2000 conditions) but it can also be used to mount crystals onto meshes to bring to the beamline.

    Negative Control:

    Article Title: A Cell-Free Fluorometric High-Throughput Screen for Inhibitors of Rtt109-Catalyzed Histone Acetylation
    Article Snippet: Briefly, test compounds and the control inhibitor ATA were first added to the appropriate wells using an Echo 550 contactless liquid handler (Labcyte). .. The KAT reaction was then initiated by dispensing 10 µL acetyl-CoA to appropriate wells to yield a final reaction concentration of 10 µM acetyl-CoA and 15 µL total solution volume per well.

    β Hematin Formation Assay:

    Article Title: Identification of β-hematin inhibitors in a high-throughput screening effort reveals scaffolds with in vitro antimalarial activity
    Article Snippet: A Labcyte Echo 550 non-contact acoustic liquid delivery system was used to deliver all control and test compounds to the 384-well assay plate. .. All test compounds (10 mM in DMSO) were added so that the final test concentration was 19.3 μM (320 total compounds tested per plate).

    In Situ:

    Article Title: Macromolecular crystallography beamline X25 at the NSLS
    Article Snippet: The Echo 550 (Labcyte, Sunnyvale, USA) is available to not only set up crystallization trays using very small volumes of protein (∼50 µL initial volume for 2000 conditions) but it can also be used to mount crystals onto meshes to bring to the beamline. .. The Echo 550 (Labcyte, Sunnyvale, USA) is available to not only set up crystallization trays using very small volumes of protein (∼50 µL initial volume for 2000 conditions) but it can also be used to mount crystals onto meshes to bring to the beamline.

    Concentration Assay:

    Article Title: 8-Substituted Pyrido[3,4-d]pyrimidin-4(3H)-one Derivatives As Potent, Cell Permeable, KDM4 (JMJD2) and KDM5 (JARID1) Histone Lysine Demethylase Inhibitors
    Article Snippet: For each compound, a 10 mM stock concentration in 100% DMSO was used. .. Compounds were dispensed using an ECHO 550 acoustic dispenser (Labcyte Inc., Sunnyvale, CA, USA) to generate 8 pt dilution curves directly into 384-well Proxiplates (no. 6008289, PerkinElmer, Waltham, MA, USA) to give final assay concentrations in the range 0.0005–100 μM in 2% (v/v) DMSO as appropriate.

    Article Title: A cellular chemical probe targeting the chromodomains of Polycomb Repressive Complex 1
    Article Snippet: The demethylase AlphaScreen® assay was performed in 384-well plate format using white proxiplates (Perkin Elmer) and compound transfers (100 nl) were performed using an ECHO 550 Acoustic Dispenser (Labcyte). .. The demethylase AlphaScreen® assay was performed in 384-well plate format using white proxiplates (Perkin Elmer) and compound transfers (100 nl) were performed using an ECHO 550 Acoustic Dispenser (Labcyte).

    Article Title: Assessing histone demethylase inhibitors in cells: lessons learned
    Article Snippet: For each compound, a 10 mM stock concentration in 100% DMSO was used. .. Compounds (100 nL) were dispensed with an ECHO® 550 acoustic dispenser (Labcyte Inc™, Sunnyvale, CA, USA) to generate 10–12 pt dilution curves directly into 384-well Proxiplates (#6008289, PerkinElmer, Waltham, MA, USA) to give final assay concentrations in the range 0.00015–30 µM in 2%(v/v) DMSO where appropriate.

    Article Title: Biased ligand quantification in drug discovery: from theory to high throughput screening to identify new biased μ opioid receptor agonists) Biased ligand quantification in drug discovery: from theory to high throughput screening to identify new biased μ opioid receptor agonists
    Article Snippet: Compounds were serially diluted in DMSO to produce 11‐point, half log concentration‐response curves in 384‐well acoustic qualified polypropylene plates (LabCyte, Sunny Vale, CA, USA). .. Assay plates were prepared as required via acoustic transfer of appropriate volumes from the serialized compound plate using an ECHO550 (LabCyte).

    Article Title: A Biochemical Screen for Identification of Small-Molecule Regulators of the Wnt Pathway Using Xenopus Egg Extracts
    Article Snippet: Samples were dispensed into chilled 384-well plates (5 µL/well) manually using a 24-channel repeat pipetter and kept in a 4 °C cold room just prior to addition of compounds. .. Library compounds were transferred by pin tool (custom apparatus, Harvard Medical School) or by Echo550 acoustic plate reformatter (Labcyte, Vanderbilt Institute of Chemical Biology) to plates at 25 °C to a final concentration ranging from ~40 to 200 µm in 2% DMSO. .. After incubation, firefly and Renilla luciferase activities were measured using the Dual-Glo Luciferase Assay (Promega) per the manufacturer’s suggested protocol.

    Article Title: Linking the Human Gut Microbiome to Inflammatory Cytokine Production Capacity
    Article Snippet: DNA samples were subsequently quantified by Quant-iT PicoGreen dsDNA Assay (Life Technologies) and normalized to a concentration of 50 pg/µL. .. Libraries were pooled by transferring equal volumes of each library using a Labcyte Echo 550 liquid handler.

    Article Title: Metformin increases glucose uptake and acts renoprotectively by reducing SHIP2 activity
    Article Snippet: Different volumes, resulting in desired final concentrations of inhibitors, were transferred to the wells of 384-well plates by using the Echo550 acoustic dispenser (Labcyte, San Jose, CA, USA). .. Recombinant protein (100 ng) in reaction buffer {10 mM HEPES, pH 7.25, 6 mM MgCl2 , 0.1% CHAPS, 250 mM sucrose, and 0.25 mM EDTA} was placed in each well (7.5 µl), and the plates were preincubated at room temperature for 15 min.

    Article Title: A Cell-Free Fluorometric High-Throughput Screen for Inhibitors of Rtt109-Catalyzed Histone Acetylation
    Article Snippet: Briefly, test compounds and the control inhibitor ATA were first added to the appropriate wells using an Echo 550 contactless liquid handler (Labcyte). .. Buffer, purified Rtt109-Vps75 and purified Asf1-dH3-H4 substrate were added in sequential dispensing steps.

    Article Title: A Fluorescence-Based Thermal Shift Assay Identifies Inhibitors of Mitogen Activated Protein Kinase Kinase 4
    Article Snippet: Small molecule screening by FTS assay was performed as described previously , but with modifications, using an Echo550 (Labcyte) for compound transfer and a Mosquito (TTP Labtech) to dispense protein into 384 well PCR plates. .. All assay experiments used 2 µg protein per well and 5 nl 5000X Sypro Orange (Invitrogen) upto a total volume of 10 µl, with a resultant protein concentration of 4.65 µM.

    Article Title: Identification of β-hematin inhibitors in a high-throughput screening effort reveals scaffolds with in vitro antimalarial activity
    Article Snippet: A Labcyte Echo 550 non-contact acoustic liquid delivery system was used to deliver all control and test compounds to the 384-well assay plate. .. A Labcyte Echo 550 non-contact acoustic liquid delivery system was used to deliver all control and test compounds to the 384-well assay plate.

    BAC Assay:

    Article Title: 8-Substituted Pyrido[3,4-d]pyrimidin-4(3H)-one Derivatives As Potent, Cell Permeable, KDM4 (JMJD2) and KDM5 (JARID1) Histone Lysine Demethylase Inhibitors
    Article Snippet: Protein production and purification: Baculovirus containing residues 1–500 of KDM4B with an N-terminal GST tag were generated according to Bac-to-Bac protocol. .. Compounds were dispensed using an ECHO 550 acoustic dispenser (Labcyte Inc., Sunnyvale, CA, USA) to generate 8 pt dilution curves directly into 384-well Proxiplates (no. 6008289, PerkinElmer, Waltham, MA, USA) to give final assay concentrations in the range 0.0005–100 μM in 2% (v/v) DMSO as appropriate.

    High Throughput Screening Assay:

    Article Title: A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids
    Article Snippet: We then prepared crystallization microplates using 2.5 nl protein solution and 2.5 nl precipitant solution using an Echo 550 (Labcyte Inc., Sunnyvale, California, USA) acoustic droplet ejection (ADE) instrument. .. We then prepared crystallization microplates using 2.5 nl protein solution and 2.5 nl precipitant solution using an Echo 550 (Labcyte Inc., Sunnyvale, California, USA) acoustic droplet ejection (ADE) instrument.

    Article Title: Cell-based high-throughput compound screening reveals functional interaction between oncofetal HMGA2 and topoisomerase I
    Article Snippet: Paragraph title: High-throughput screening ... After 24 h, cells from identical co-transfections were pooled, and about 10 000 cells were plated per well on a 384-well white plate with clear-bottom (Greiner bio one) using the LABCYTE Echo 550 liquid handler.

    Article Title: Metformin increases glucose uptake and acts renoprotectively by reducing SHIP2 activity
    Article Snippet: The catalytic activities of the recombinant SHIP2 and SHIP1 phosphatase domains were determined by malachite green phosphate assay at the High Throughput Biomedicine Unit (University of Helsinki) using d -myo-PI(3,4,5)P3 (Echelon Biosciences, Salt Lake City, UT, USA). .. Different volumes, resulting in desired final concentrations of inhibitors, were transferred to the wells of 384-well plates by using the Echo550 acoustic dispenser (Labcyte, San Jose, CA, USA).

    Article Title: A Cell-Free Fluorometric High-Throughput Screen for Inhibitors of Rtt109-Catalyzed Histone Acetylation
    Article Snippet: Paragraph title: High-throughput screen in 384-well plate format ... Briefly, test compounds and the control inhibitor ATA were first added to the appropriate wells using an Echo 550 contactless liquid handler (Labcyte).

    Article Title: Identification of β-hematin inhibitors in a high-throughput screening effort reveals scaffolds with in vitro antimalarial activity
    Article Snippet: A Labcyte Echo 550 non-contact acoustic liquid delivery system was used to deliver all control and test compounds to the 384-well assay plate. .. A Labcyte Echo 550 non-contact acoustic liquid delivery system was used to deliver all control and test compounds to the 384-well assay plate.

    Recombinant:

    Article Title: Metformin increases glucose uptake and acts renoprotectively by reducing SHIP2 activity
    Article Snippet: The catalytic activities of the recombinant SHIP2 and SHIP1 phosphatase domains were determined by malachite green phosphate assay at the High Throughput Biomedicine Unit (University of Helsinki) using d -myo-PI(3,4,5)P3 (Echelon Biosciences, Salt Lake City, UT, USA). .. Different volumes, resulting in desired final concentrations of inhibitors, were transferred to the wells of 384-well plates by using the Echo550 acoustic dispenser (Labcyte, San Jose, CA, USA).

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    Labcyte echo 550 liquid handling instrument
    Acoustic signatures of diverse plates. The Echo 550 was used to ‘ping’ each of the polypropylene (polypro) assemblies and to record the acoustic echo from the components of each assembly. The acoustically compatible polypropylene source plate ( a ) exhibits two modest reflections from the plate bottom (left) and a strong reflection at the liquid–air interface (right). This strong pulse is needed for crystal ejection. The MiTeGen  In Situ -1 plate ( b ) and the CrystalDirect plate ( c ) reflected a modest amount of energy (middle) but sufficient power was retained at the surface to eject crystals. In contrast, three plate designs experienced an excessive loss of energy and there was insufficient acoustic power at the surface to eject crystals. The two Greiner plates ( d ,  e ) lost significant energy through reflection. In contrast, scattering must account for most of the power loss in the Intelli-Plate ( f ) since there were no audible reflections.
    Echo 550 Liquid Handling Instrument, supplied by Labcyte, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Acoustic signatures of diverse plates. The Echo 550 was used to ‘ping’ each of the polypropylene (polypro) assemblies and to record the acoustic echo from the components of each assembly. The acoustically compatible polypropylene source plate ( a ) exhibits two modest reflections from the plate bottom (left) and a strong reflection at the liquid–air interface (right). This strong pulse is needed for crystal ejection. The MiTeGen  In Situ -1 plate ( b ) and the CrystalDirect plate ( c ) reflected a modest amount of energy (middle) but sufficient power was retained at the surface to eject crystals. In contrast, three plate designs experienced an excessive loss of energy and there was insufficient acoustic power at the surface to eject crystals. The two Greiner plates ( d ,  e ) lost significant energy through reflection. In contrast, scattering must account for most of the power loss in the Intelli-Plate ( f ) since there were no audible reflections.

    Journal: Acta Crystallographica. Section D, Structural Biology

    Article Title: Using sound pulses to solve the crystal-harvesting bottleneck

    doi: 10.1107/S2059798318011506

    Figure Lengend Snippet: Acoustic signatures of diverse plates. The Echo 550 was used to ‘ping’ each of the polypropylene (polypro) assemblies and to record the acoustic echo from the components of each assembly. The acoustically compatible polypropylene source plate ( a ) exhibits two modest reflections from the plate bottom (left) and a strong reflection at the liquid–air interface (right). This strong pulse is needed for crystal ejection. The MiTeGen In Situ -1 plate ( b ) and the CrystalDirect plate ( c ) reflected a modest amount of energy (middle) but sufficient power was retained at the surface to eject crystals. In contrast, three plate designs experienced an excessive loss of energy and there was insufficient acoustic power at the surface to eject crystals. The two Greiner plates ( d , e ) lost significant energy through reflection. In contrast, scattering must account for most of the power loss in the Intelli-Plate ( f ) since there were no audible reflections.

    Article Snippet: We demonstrate that a commercially available Echo 550 liquid-handling instrument (Labcyte Inc., Sunnyvale, California, USA) can be used to harvest protein crystals from slightly modified MiTeGen In Situ -1 plates.

    Techniques: In Situ

    Click to mount: ejecting a selected crystal cluster. We selected a cluster of three crystals and carefully aligned these crystals with the ejection zone. We then used the Echo 550 to harvest these crystals onto a micro-mesh. ( a ) shows a view of the crystallization well; ( b ) shows a micro-mesh image.

    Journal: Acta Crystallographica. Section D, Structural Biology

    Article Title: Using sound pulses to solve the crystal-harvesting bottleneck

    doi: 10.1107/S2059798318011506

    Figure Lengend Snippet: Click to mount: ejecting a selected crystal cluster. We selected a cluster of three crystals and carefully aligned these crystals with the ejection zone. We then used the Echo 550 to harvest these crystals onto a micro-mesh. ( a ) shows a view of the crystallization well; ( b ) shows a micro-mesh image.

    Article Snippet: We demonstrate that a commercially available Echo 550 liquid-handling instrument (Labcyte Inc., Sunnyvale, California, USA) can be used to harvest protein crystals from slightly modified MiTeGen In Situ -1 plates.

    Techniques: Crystallization Assay

    Overview of the apparatus for acoustic ejection from non-acoustic labware. For acoustic ejection of protein crystals to take place, the distance between the acoustic transducer in the Echo 550 and the bottom of the crystallization plate must equal one of two possible preset values. To accomplish this, crystallization plates must either be cut and placed on a plate of the correct height ( a ), be combined with a spacer ( b ) or be lightly sanded ( c ). There are no similar limits to the size of the destination plate ( d ). ( a ) For initial testing, the Echo 550 was used to harvest crystals from a polypropylene assembly which contained fragments of different commercially available plates (inset). ( b ) To test the harvesting of crystals grown inside the most promising crystallization phase, it was coupled to a thin slice from an acoustically transparent plate (inset). ( c ) Finally, intact MiTeGen plates were used to grow and harvest protein crystals by lightly sanding down the edge pedestal (inset). ( d ) Acoustically harvested crystals were transferred to a pin platform that contained up to 96 micro-meshes. The crystals on the micro-meshes (inset) were then pressure-fitted into a MiTeGen Reusable Base (model B1A-R) and cryocooled.

    Journal: Acta Crystallographica. Section D, Structural Biology

    Article Title: Using sound pulses to solve the crystal-harvesting bottleneck

    doi: 10.1107/S2059798318011506

    Figure Lengend Snippet: Overview of the apparatus for acoustic ejection from non-acoustic labware. For acoustic ejection of protein crystals to take place, the distance between the acoustic transducer in the Echo 550 and the bottom of the crystallization plate must equal one of two possible preset values. To accomplish this, crystallization plates must either be cut and placed on a plate of the correct height ( a ), be combined with a spacer ( b ) or be lightly sanded ( c ). There are no similar limits to the size of the destination plate ( d ). ( a ) For initial testing, the Echo 550 was used to harvest crystals from a polypropylene assembly which contained fragments of different commercially available plates (inset). ( b ) To test the harvesting of crystals grown inside the most promising crystallization phase, it was coupled to a thin slice from an acoustically transparent plate (inset). ( c ) Finally, intact MiTeGen plates were used to grow and harvest protein crystals by lightly sanding down the edge pedestal (inset). ( d ) Acoustically harvested crystals were transferred to a pin platform that contained up to 96 micro-meshes. The crystals on the micro-meshes (inset) were then pressure-fitted into a MiTeGen Reusable Base (model B1A-R) and cryocooled.

    Article Snippet: We demonstrate that a commercially available Echo 550 liquid-handling instrument (Labcyte Inc., Sunnyvale, California, USA) can be used to harvest protein crystals from slightly modified MiTeGen In Situ -1 plates.

    Techniques: Crystallization Assay

    Acoustic droplet ejection (ADE). ADE uses sound energy to transfer variable micro-droplets ( e.g.  nanolitres or picolitres) of solution (including suspended solids) from a crystallization well, through a short air column (∼1 cm) to data-collection media. Sound-wave energy from the transducer is channelled to the focal point ( i.e.  the ejection zone), displacing the surface, where a controlled ejection occurs. Droplet size is governed by the wavelength of the sound emitted and this proportionality yields accurate ejected volumes. In this work, an Echo 550 liquid handler was used to harvest protein crystals from two kinds of  in situ  plates (MiTeGen  In Situ -1) onto MiTeGen MicroMeshes.

    Journal: Acta Crystallographica. Section D, Structural Biology

    Article Title: Using sound pulses to solve the crystal-harvesting bottleneck

    doi: 10.1107/S2059798318011506

    Figure Lengend Snippet: Acoustic droplet ejection (ADE). ADE uses sound energy to transfer variable micro-droplets ( e.g. nanolitres or picolitres) of solution (including suspended solids) from a crystallization well, through a short air column (∼1 cm) to data-collection media. Sound-wave energy from the transducer is channelled to the focal point ( i.e. the ejection zone), displacing the surface, where a controlled ejection occurs. Droplet size is governed by the wavelength of the sound emitted and this proportionality yields accurate ejected volumes. In this work, an Echo 550 liquid handler was used to harvest protein crystals from two kinds of in situ plates (MiTeGen In Situ -1) onto MiTeGen MicroMeshes.

    Article Snippet: We demonstrate that a commercially available Echo 550 liquid-handling instrument (Labcyte Inc., Sunnyvale, California, USA) can be used to harvest protein crystals from slightly modified MiTeGen In Situ -1 plates.

    Techniques: Crystallization Assay, In Situ

    A grid adaptor for liquid-handling robots. ( a ) A grid adaptor with a standard microplate footprint allows grids to be positioned in the plate position of liquid-handling robots, including the ARI Gryphon and the Labcyte Echo 550 liquid handler. A neoprene-lined torsion clip (black) grips the magnetic base and holds the grid in position. ( b ) The grid is indexed against two metal surfaces that protrude from the adaptor to ensure that the ports are correctly positioned. ( c ) A photograph of the grid adaptor holding a grid in the destination-plate position of a Labcyte Echo 550 liquid-handling robot.

    Journal: Acta Crystallographica. Section D, Structural Biology

    Article Title: High-density grids for efficient data collection from multiple crystals

    doi: 10.1107/S2059798315020847

    Figure Lengend Snippet: A grid adaptor for liquid-handling robots. ( a ) A grid adaptor with a standard microplate footprint allows grids to be positioned in the plate position of liquid-handling robots, including the ARI Gryphon and the Labcyte Echo 550 liquid handler. A neoprene-lined torsion clip (black) grips the magnetic base and holds the grid in position. ( b ) The grid is indexed against two metal surfaces that protrude from the adaptor to ensure that the ports are correctly positioned. ( c ) A photograph of the grid adaptor holding a grid in the destination-plate position of a Labcyte Echo 550 liquid-handling robot.

    Article Snippet: The adaptor has been successfully tested with the Labcyte Echo 550 (Fig. 2 c ), the Art Robbins Gryphon (Supplementary Video S1) and the TTP Labtech Mosquito (Supplementary Video S2).

    Techniques: Cross-linking Immunoprecipitation

    ( a ) An edge-on view of a grid with polycarbonate backing during data collection at LCLS-XPP. An Echo 550 liquid-handling robot was used to position droplets of a perforin crystal suspension inline with grid ports immediately prior to flash-cooling in liquid nitrogen. ( b ) Two crystals of perforin positioned over a grid port during data collection at LCLS-XPP. A hole is clearly visible in the top crystal where it has been exposed to the X-ray beam. The bottom crystal is still intact.

    Journal: Acta Crystallographica. Section D, Structural Biology

    Article Title: High-density grids for efficient data collection from multiple crystals

    doi: 10.1107/S2059798315020847

    Figure Lengend Snippet: ( a ) An edge-on view of a grid with polycarbonate backing during data collection at LCLS-XPP. An Echo 550 liquid-handling robot was used to position droplets of a perforin crystal suspension inline with grid ports immediately prior to flash-cooling in liquid nitrogen. ( b ) Two crystals of perforin positioned over a grid port during data collection at LCLS-XPP. A hole is clearly visible in the top crystal where it has been exposed to the X-ray beam. The bottom crystal is still intact.

    Article Snippet: The adaptor has been successfully tested with the Labcyte Echo 550 (Fig. 2 c ), the Art Robbins Gryphon (Supplementary Video S1) and the TTP Labtech Mosquito (Supplementary Video S2).

    Techniques:

    Representative result of high-throughput screening of BSH inhibitors. (A) Plate layout for screening BSH inhibitors. Pink boxes (columns 3–22) indicate test wells that contain library compounds of interest, BSH, and reaction mix containing substrate. Library compounds were shot into the well bottom using Echo 550/555 and enzyme and reaction mix were added using Multidrop Combi. Controls were added manually to the side wells (columns 1–2 and 23–24): blue boxes indicate activity controls (BSH, reaction mix containing substrate, and solvent DMSO), yellow boxes correspond to inhibition controls (BSH, reaction mix containing substrate, and NaIO 3 ), and white boxes are negative controls with no BSH added but include reaction mix as well as substrate. (B) The HTS results represented by one 384-well plate. Control wells indicate the assay proceeded normally. The wells in columns 3–22 that appeared clear, regardless of alternative color due to compound, and had low absorbance readings were considered hits (putative BSH inhibitors).

    Journal: PLoS ONE

    Article Title: Discovery of Bile Salt Hydrolase Inhibitors Using an Efficient High-Throughput Screening System

    doi: 10.1371/journal.pone.0085344

    Figure Lengend Snippet: Representative result of high-throughput screening of BSH inhibitors. (A) Plate layout for screening BSH inhibitors. Pink boxes (columns 3–22) indicate test wells that contain library compounds of interest, BSH, and reaction mix containing substrate. Library compounds were shot into the well bottom using Echo 550/555 and enzyme and reaction mix were added using Multidrop Combi. Controls were added manually to the side wells (columns 1–2 and 23–24): blue boxes indicate activity controls (BSH, reaction mix containing substrate, and solvent DMSO), yellow boxes correspond to inhibition controls (BSH, reaction mix containing substrate, and NaIO 3 ), and white boxes are negative controls with no BSH added but include reaction mix as well as substrate. (B) The HTS results represented by one 384-well plate. Control wells indicate the assay proceeded normally. The wells in columns 3–22 that appeared clear, regardless of alternative color due to compound, and had low absorbance readings were considered hits (putative BSH inhibitors).

    Article Snippet: Briefly, 0.25 µl of a specific compound from the Spectrum Collection library was transferred into the bottom of a clear 384-well microplate (Greiner cat # 781182) with flat bottom by using the Echo 550/555 Liquid Handlers (Labcyte).

    Techniques: High Throughput Screening Assay, Activity Assay, Inhibition

    Plate lids result in better and more reproducible crystals. The figure shows crystals that were grown using 2.5 nl precipitant solution and 2.5 nl lysozyme ( a ) and trypsin ( b ) solution in CrystalQuick plates (approximately 2½ min for the Echo 550 to prepare each plate). Each type of protein was prepared on a plate that had a lid and on a plate that did not have a plate lid. On average, using a plate lid resulted in fewer but larger crystals of lysozyme and trypsin (left). The number of thaumatin crystals was approximately constant. X-ray data were obtained from one of the lysozyme crystals in each well in the middle row of the plate (row  D ). The data from crystals prepared using lids were merged and compared with the data from crystals prepared without lids. We also used the technique described in § 2.1 to measure the evaporation rate for each of the three types of mother liquor with no plate lid, and compared this with the deduced evaporation rate with a plate lid (right). Using lids during the 2½ min required for the Echo 550 to set up the 5 nl crystallization droplets allowed better, more reproducible and better diffracting crystals to be obtained.

    Journal: Acta Crystallographica. Section F, Structural Biology Communications

    Article Title: A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids

    doi: 10.1107/S2053230X14025126

    Figure Lengend Snippet: Plate lids result in better and more reproducible crystals. The figure shows crystals that were grown using 2.5 nl precipitant solution and 2.5 nl lysozyme ( a ) and trypsin ( b ) solution in CrystalQuick plates (approximately 2½ min for the Echo 550 to prepare each plate). Each type of protein was prepared on a plate that had a lid and on a plate that did not have a plate lid. On average, using a plate lid resulted in fewer but larger crystals of lysozyme and trypsin (left). The number of thaumatin crystals was approximately constant. X-ray data were obtained from one of the lysozyme crystals in each well in the middle row of the plate (row D ). The data from crystals prepared using lids were merged and compared with the data from crystals prepared without lids. We also used the technique described in § 2.1 to measure the evaporation rate for each of the three types of mother liquor with no plate lid, and compared this with the deduced evaporation rate with a plate lid (right). Using lids during the 2½ min required for the Echo 550 to set up the 5 nl crystallization droplets allowed better, more reproducible and better diffracting crystals to be obtained.

    Article Snippet: We then prepared crystallization microplates using 2.5 nl protein solution and 2.5 nl precipitant solution using an Echo 550 (Labcyte Inc., Sunnyvale, California, USA) acoustic droplet ejection (ADE) instrument.

    Techniques: Evaporation, Crystallization Assay