echo525 instrument  (Labcyte)


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    Name:
    Echo 525 Liquid Handler
    Description:
    The newest member of the Echo product family enables rapid higher volume transfer of protein based and biochemical reagents With a 25 nL increment and rapid transfer rates the Echo 525 Liquid Handler transfers reagents contamination free without sacrificing reagent volume or reagent quality to tubing tips or nozzles
    Catalog Number:
    001-10080
    Price:
    None
    Category:
    Liquid handler
    Score:
    85
    Quantity:
    1
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    Structured Review

    Labcyte echo525 instrument
    The newest member of the Echo product family enables rapid higher volume transfer of protein based and biochemical reagents With a 25 nL increment and rapid transfer rates the Echo 525 Liquid Handler transfers reagents contamination free without sacrificing reagent volume or reagent quality to tubing tips or nozzles
    https://www.bioz.com/result/echo525 instrument/product/Labcyte
    Average 84 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    echo525 instrument - by Bioz Stars, 2019-10
    84/100 stars

    Related Products / Commonly Used Together

    mastermix Roche
    echo525 dispenser Labcyte

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    Related Articles

    Clone Assay:

    Article Title: Genome-wide identification of bacterial plant colonization genes
    Article Snippet: Paragraph title: Arraying and selecting clones from the library ... To unambiguously determine the barcode identity and plate address of all the mutants in this collection, 25 nL from each well of the 384-well plates was transferred using an Echo525 instrument (Labcyte, San Jose, CA) to a new well on a 96-well plate based on a multiplexing strategy involving pooling of rows, columns, and plates.

    Amplification:

    Article Title: The ace-1 Locus Is Amplified in All Resistant Anopheles gambiae Mosquitoes: Fitness Consequences of Homogeneous and Heterogeneous Duplications
    Article Snippet: We dispensed 0.5 μl of genomic DNA and 1.5 μl of reaction mixture containing specific primers, each at a concentration of 0.8 μM and 0.75 μl of Master Mix (LightCycler 480 SYBR Green I Master, Roche) into the wells of a 384-well plate, with a Labcyte Echo525 dispenser. .. We dispensed 0.5 μl of genomic DNA and 1.5 μl of reaction mixture containing specific primers, each at a concentration of 0.8 μM and 0.75 μl of Master Mix (LightCycler 480 SYBR Green I Master, Roche) into the wells of a 384-well plate, with a Labcyte Echo525 dispenser.

    Article Title: Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae, et al. Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae
    Article Snippet: We used the primer pair AgAce1qtidir2 and AgAce1qtirev2 primers to amplify a 185‐bp fragment of the ace‐1 gene (Table ), and the primer pair Del97Qdir5 and Del97Qrev4 to amplify a 186‐bp fragment overlapping the ID (Table ). .. We used the qPCR amplification conditions described by Assogba et al. ( ): 0.5 μl of genomic DNA and 1.5 μl of reaction mix containing 0.8 μM of each specific primer and 0.75 μl of mastermix (LightCycler® 480 SYBR Green I Master, Roche) were dispensed on a 384‐well plate using the Labcyte® Echo525 dispenser. .. The qPCR was performed with a 95°C activation step for 8 min followed by 45 cycles of 95°C for 4‐s, 67°C for 13 s, and 72°C for 19 s. Melting curves were generated by a postamplification melting step between 70°C and 95°C, for Tm analysis.

    Article Title: Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?
    Article Snippet: The qPCR assay was prepared in Roche 384 well PCR plates (product no. 04729749001) in a 10 μl reaction volume fired using an ECHO 525 non-contact micro dispenser (LabCyte). .. Three primer probe pairs were used in each assay against the following housekeeping genes: RPLP0 (CY5), RPL19 (FAM) and ACTB (CYAN500) using three unique fluorophores compatible with the Roche LightCycler480 PCR machine.

    Construct:

    Article Title: The ace-1 Locus Is Amplified in All Resistant Anopheles gambiae Mosquitoes: Fitness Consequences of Homogeneous and Heterogeneous Duplications
    Article Snippet: We dispensed 0.5 μl of genomic DNA and 1.5 μl of reaction mixture containing specific primers, each at a concentration of 0.8 μM and 0.75 μl of Master Mix (LightCycler 480 SYBR Green I Master, Roche) into the wells of a 384-well plate, with a Labcyte Echo525 dispenser. .. We dispensed 0.5 μl of genomic DNA and 1.5 μl of reaction mixture containing specific primers, each at a concentration of 0.8 μM and 0.75 μl of Master Mix (LightCycler 480 SYBR Green I Master, Roche) into the wells of a 384-well plate, with a Labcyte Echo525 dispenser.

    Article Title: Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae, et al. Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae
    Article Snippet: We used the qPCR amplification conditions described by Assogba et al. ( ): 0.5 μl of genomic DNA and 1.5 μl of reaction mix containing 0.8 μM of each specific primer and 0.75 μl of mastermix (LightCycler® 480 SYBR Green I Master, Roche) were dispensed on a 384‐well plate using the Labcyte® Echo525 dispenser. .. We used the qPCR amplification conditions described by Assogba et al. ( ): 0.5 μl of genomic DNA and 1.5 μl of reaction mix containing 0.8 μM of each specific primer and 0.75 μl of mastermix (LightCycler® 480 SYBR Green I Master, Roche) were dispensed on a 384‐well plate using the Labcyte® Echo525 dispenser.

    SYBR Green Assay:

    Article Title: Salinity stress from the perspective of the energy-redox axis: Lessons from a marine intertidal flatworm
    Article Snippet: Reverse transcription was carried out using M-MLV Reverse Transcriptase (Invitrogen, France). .. Using an Echo® 525 liquid handling system (Labcyte Inc., California, USA), 0.75 µl of LightCycler-FastStart DNA Master SYBR-Green I™ Mix (Roche, Mannheim, Germany), 0.015 µl of each primer (forward and reverse at 0.2 µM final concentration), 0.22 µl of ultra-pure water and 0.5 µl of cDNA were dispensed into a 384-well reaction plate. .. The qPCR conditions were as follows: denaturation at 95 °C for 10 min, followed by 45 cycles of repeat amplification (95 °C, 15 s), hybridization (60 °C or 62 °C according to the primer pair used, 5 s) and elongation (72 °C, 10 s) and a final step at 40 °C for 30 s. A melting curve program was performed to control the amplification specificity.

    Article Title: The ace-1 Locus Is Amplified in All Resistant Anopheles gambiae Mosquitoes: Fitness Consequences of Homogeneous and Heterogeneous Duplications
    Article Snippet: As they are located outside the amplicon, they should not be amplified. .. We dispensed 0.5 μl of genomic DNA and 1.5 μl of reaction mixture containing specific primers, each at a concentration of 0.8 μM and 0.75 μl of Master Mix (LightCycler 480 SYBR Green I Master, Roche) into the wells of a 384-well plate, with a Labcyte Echo525 dispenser. .. We performed qPCR as follows: activation at 95°C for 8 min, followed by 45 cycles of 95°C for 4 s, 67°C for 13 s, and 72°C for 19 s. Melting curves were generated by a post-amplification melting step between 70°C and 95°C, for Tm analysis.

    Article Title: Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae, et al. Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae
    Article Snippet: We used the primer pair AgAce1qtidir2 and AgAce1qtirev2 primers to amplify a 185‐bp fragment of the ace‐1 gene (Table ), and the primer pair Del97Qdir5 and Del97Qrev4 to amplify a 186‐bp fragment overlapping the ID (Table ). .. We used the qPCR amplification conditions described by Assogba et al. ( ): 0.5 μl of genomic DNA and 1.5 μl of reaction mix containing 0.8 μM of each specific primer and 0.75 μl of mastermix (LightCycler® 480 SYBR Green I Master, Roche) were dispensed on a 384‐well plate using the Labcyte® Echo525 dispenser. .. The qPCR was performed with a 95°C activation step for 8 min followed by 45 cycles of 95°C for 4‐s, 67°C for 13 s, and 72°C for 19 s. Melting curves were generated by a postamplification melting step between 70°C and 95°C, for Tm analysis.

    Incubation:

    Article Title: Genome-wide identification of bacterial plant colonization genes
    Article Snippet: To unambiguously determine the barcode identity and plate address of all the mutants in this collection, 25 nL from each well of the 384-well plates was transferred using an Echo525 instrument (Labcyte, San Jose, CA) to a new well on a 96-well plate based on a multiplexing strategy involving pooling of rows, columns, and plates. .. To unambiguously determine the barcode identity and plate address of all the mutants in this collection, 25 nL from each well of the 384-well plates was transferred using an Echo525 instrument (Labcyte, San Jose, CA) to a new well on a 96-well plate based on a multiplexing strategy involving pooling of rows, columns, and plates.

    Article Title: Development and characterisation of a novel glucagon like peptide-1 receptor antibody
    Article Snippet: Serial dilutions of the antibody and control peptides were prepared in assay buffer and plated using an ECHO525 acoustic liquid handler (Labcyte, Sunnyvale, CA, USA) to give an 11 point dose–response curve in duplicate. .. Serial dilutions of the antibody and control peptides were prepared in assay buffer and plated using an ECHO525 acoustic liquid handler (Labcyte, Sunnyvale, CA, USA) to give an 11 point dose–response curve in duplicate.

    Article Title: Computational-experimental approach to drug-target interaction mapping: A case study on kinase inhibitors
    Article Snippet: The kinase inhibitors were dissolved to 1X Kinase Buffer with 5% DMSO by transferring 200nL of the buffer using ECHO525 Liquid handler (Labcyte). .. The kinase inhibitors were dissolved to 1X Kinase Buffer with 5% DMSO by transferring 200nL of the buffer using ECHO525 Liquid handler (Labcyte).

    Article Title: Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?
    Article Snippet: 7 μl of RNA at a concentration of 5 ng/μl (quantified using the Qubit 2.0 fluorometer) was used to generate cDNA in a 10 μl total extraction volume using the SuperScript VILO cDNA synthesis kit (Life Technologies, Catalogue Number 11754-050) followed by incubation at 25 °C for 10 min, 42 °C for 60 min and 85 °C for 5 min. .. The qPCR assay was prepared in Roche 384 well PCR plates (product no. 04729749001) in a 10 μl reaction volume fired using an ECHO 525 non-contact micro dispenser (LabCyte).

    Activity Assay:

    Article Title: Development and characterisation of a novel glucagon like peptide-1 receptor antibody
    Article Snippet: Cell-based cAMP HTRF accumulation assays were used to screen Glp1R0017 for activity in overexpressing cell lines. .. Serial dilutions of the antibody and control peptides were prepared in assay buffer and plated using an ECHO525 acoustic liquid handler (Labcyte, Sunnyvale, CA, USA) to give an 11 point dose–response curve in duplicate.

    Modification:

    Article Title: A novel prospective isolation of murine fetal liver progenitors to study in utero hematopoietic defects
    Article Snippet: Libraries were prepared using a modified version of the Smart-Seq2 protocol [ ]. .. Dual indexed sequencing libraries were prepared from 0.1ng cDNA using an Echo525 automation system (Labcyte) in miniaturized reaction volumes.

    Derivative Assay:

    Article Title: A novel prospective isolation of murine fetal liver progenitors to study in utero hematopoietic defects
    Article Snippet: Dual indexed sequencing libraries were prepared from 0.1ng cDNA using an Echo525 automation system (Labcyte) in miniaturized reaction volumes. .. Base call files generated from the NextSeq 500 sequencing run were converted to the FASTQ format with the bcl2fast converter (Illumina).

    Flow Cytometry:

    Article Title: A novel prospective isolation of murine fetal liver progenitors to study in utero hematopoietic defects
    Article Snippet: E14.5 P1-GFP ::P2-hCD4/+ fetal livers were prepared for flow sorting as described above. .. Dual indexed sequencing libraries were prepared from 0.1ng cDNA using an Echo525 automation system (Labcyte) in miniaturized reaction volumes.

    Transferring:

    Article Title: Computational-experimental approach to drug-target interaction mapping: A case study on kinase inhibitors
    Article Snippet: The inhibitors were transferred at 7.5 and 2.5 nL volumes from Echo Qualified 384-Well Low Dead Volume Microplates (Labcyte, LP-0200). .. The kinase inhibitors were dissolved to 1X Kinase Buffer with 5% DMSO by transferring 200nL of the buffer using ECHO525 Liquid handler (Labcyte). .. After the transfer, the assay plate was immediately sealed to minimize evaporation, and centrifuged at 872rcf/2min/RT.

    Digital PCR:

    Article Title: Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae, et al. Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae
    Article Snippet: We used the qPCR amplification conditions described by Assogba et al. ( ): 0.5 μl of genomic DNA and 1.5 μl of reaction mix containing 0.8 μM of each specific primer and 0.75 μl of mastermix (LightCycler® 480 SYBR Green I Master, Roche) were dispensed on a 384‐well plate using the Labcyte® Echo525 dispenser. .. Standard curves were constructed using 10 to 10 dilutions of a PCR product previously amplified: (i) on KisumuP (SS) strain DNA for ace‐1 and RpS7 specific primers and (ii) on AcerkisR3 (R3 *R3 *) strain DNA for the ID region‐specific primers (this strain present a single ID on one of the three ace‐1 ‐encompassing amplicons carried by each chromosome, Assogba et al., ). ace‐1 and ID copy‐number ratios over RpS7 were determined using the advanced relative quantification method (LightCycler® 480 software v.1.5.0).

    Generated:

    Article Title: Rapid acquisition and model-based analysis of cell-free transcription–translation reactions from nonmodel bacteria
    Article Snippet: Calibration standards of purified GFP-MGapt mRNA, His6 -tagged GFP and mCherry were prepared in the standard reaction mixture (SI Appendix , Figs. S2 and S14 ). .. Full factorial experimental design and Echo525 liquid handler (Labcyte) pick list transfer instructions were automatically generated using a Python script (available at https://github.com/jmacdona/ODE_MCMC_tools ) or Labcyte PickList software. .. Liquid droplets were transferred as multiples of 25 nL to a final volume of 10 μL as three triplicate replicates.

    Article Title: A novel prospective isolation of murine fetal liver progenitors to study in utero hematopoietic defects
    Article Snippet: Dual indexed sequencing libraries were prepared from 0.1ng cDNA using an Echo525 automation system (Labcyte) in miniaturized reaction volumes. .. Paired-end 75bp sequencing was carried out by clustering 1.5pM of the library pool on a NextSeq 500 sequencer (Illumina).

    Sequencing:

    Article Title: A novel prospective isolation of murine fetal liver progenitors to study in utero hematopoietic defects
    Article Snippet: Briefly, cDNA was prepared using a Mantis platform (Formulatrix) and quantified with quantIT picogreen reagent (Thermo Fisher). .. Dual indexed sequencing libraries were prepared from 0.1ng cDNA using an Echo525 automation system (Labcyte) in miniaturized reaction volumes. .. The library pool was quantified by qPCR using a Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems).

    Article Title: Genome-wide identification of bacterial plant colonization genes
    Article Snippet: To unambiguously determine the barcode identity and plate address of all the mutants in this collection, 25 nL from each well of the 384-well plates was transferred using an Echo525 instrument (Labcyte, San Jose, CA) to a new well on a 96-well plate based on a multiplexing strategy involving pooling of rows, columns, and plates. .. To unambiguously determine the barcode identity and plate address of all the mutants in this collection, 25 nL from each well of the 384-well plates was transferred using an Echo525 instrument (Labcyte, San Jose, CA) to a new well on a 96-well plate based on a multiplexing strategy involving pooling of rows, columns, and plates.

    Multiplexing:

    Article Title: Genome-wide identification of bacterial plant colonization genes
    Article Snippet: Aliquots were taken for downstream processes and the 384-well plate was kept at −80°C. .. To unambiguously determine the barcode identity and plate address of all the mutants in this collection, 25 nL from each well of the 384-well plates was transferred using an Echo525 instrument (Labcyte, San Jose, CA) to a new well on a 96-well plate based on a multiplexing strategy involving pooling of rows, columns, and plates. .. 1.6–4.8 μl of each pooled culture within the 96-well plate was used as input for RB-TnSeq.

    RNA Sequencing Assay:

    Article Title: A novel prospective isolation of murine fetal liver progenitors to study in utero hematopoietic defects
    Article Snippet: Paragraph title: Single cell RNA sequencing ... Dual indexed sequencing libraries were prepared from 0.1ng cDNA using an Echo525 automation system (Labcyte) in miniaturized reaction volumes.

    Fluorescence:

    Article Title: Rapid acquisition and model-based analysis of cell-free transcription–translation reactions from nonmodel bacteria
    Article Snippet: Full factorial experimental design and Echo525 liquid handler (Labcyte) pick list transfer instructions were automatically generated using a Python script (available at https://github.com/jmacdona/ODE_MCMC_tools ) or Labcyte PickList software. .. Full factorial experimental design and Echo525 liquid handler (Labcyte) pick list transfer instructions were automatically generated using a Python script (available at https://github.com/jmacdona/ODE_MCMC_tools ) or Labcyte PickList software.

    RNA Extraction:

    Article Title: Salinity stress from the perspective of the energy-redox axis: Lessons from a marine intertidal flatworm
    Article Snippet: Paragraph title: RNA extraction and quantification of transcript levels ... Using an Echo® 525 liquid handling system (Labcyte Inc., California, USA), 0.75 µl of LightCycler-FastStart DNA Master SYBR-Green I™ Mix (Roche, Mannheim, Germany), 0.015 µl of each primer (forward and reverse at 0.2 µM final concentration), 0.22 µl of ultra-pure water and 0.5 µl of cDNA were dispensed into a 384-well reaction plate.

    Polymerase Chain Reaction:

    Article Title: The ace-1 Locus Is Amplified in All Resistant Anopheles gambiae Mosquitoes: Fitness Consequences of Homogeneous and Heterogeneous Duplications
    Article Snippet: We dispensed 0.5 μl of genomic DNA and 1.5 μl of reaction mixture containing specific primers, each at a concentration of 0.8 μM and 0.75 μl of Master Mix (LightCycler 480 SYBR Green I Master, Roche) into the wells of a 384-well plate, with a Labcyte Echo525 dispenser. .. We dispensed 0.5 μl of genomic DNA and 1.5 μl of reaction mixture containing specific primers, each at a concentration of 0.8 μM and 0.75 μl of Master Mix (LightCycler 480 SYBR Green I Master, Roche) into the wells of a 384-well plate, with a Labcyte Echo525 dispenser.

    Article Title: Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae, et al. Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae
    Article Snippet: We used the qPCR amplification conditions described by Assogba et al. ( ): 0.5 μl of genomic DNA and 1.5 μl of reaction mix containing 0.8 μM of each specific primer and 0.75 μl of mastermix (LightCycler® 480 SYBR Green I Master, Roche) were dispensed on a 384‐well plate using the Labcyte® Echo525 dispenser. .. We used the qPCR amplification conditions described by Assogba et al. ( ): 0.5 μl of genomic DNA and 1.5 μl of reaction mix containing 0.8 μM of each specific primer and 0.75 μl of mastermix (LightCycler® 480 SYBR Green I Master, Roche) were dispensed on a 384‐well plate using the Labcyte® Echo525 dispenser.

    Article Title: Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?
    Article Snippet: 7 μl of RNA at a concentration of 5 ng/μl (quantified using the Qubit 2.0 fluorometer) was used to generate cDNA in a 10 μl total extraction volume using the SuperScript VILO cDNA synthesis kit (Life Technologies, Catalogue Number 11754-050) followed by incubation at 25 °C for 10 min, 42 °C for 60 min and 85 °C for 5 min. .. The qPCR assay was prepared in Roche 384 well PCR plates (product no. 04729749001) in a 10 μl reaction volume fired using an ECHO 525 non-contact micro dispenser (LabCyte). .. Three primer probe pairs were used in each assay against the following housekeeping genes: RPLP0 (CY5), RPL19 (FAM) and ACTB (CYAN500) using three unique fluorophores compatible with the Roche LightCycler480 PCR machine.

    Chromatin Immunoprecipitation:

    Article Title: Salinity stress from the perspective of the energy-redox axis: Lessons from a marine intertidal flatworm
    Article Snippet: RNA concentration and integrity were assessed using an Agilent Bioanalyzer 2100 equipped with an RNA Nano Chip (Agilent Technologies, CA, USA). .. Using an Echo® 525 liquid handling system (Labcyte Inc., California, USA), 0.75 µl of LightCycler-FastStart DNA Master SYBR-Green I™ Mix (Roche, Mannheim, Germany), 0.015 µl of each primer (forward and reverse at 0.2 µM final concentration), 0.22 µl of ultra-pure water and 0.5 µl of cDNA were dispensed into a 384-well reaction plate.

    Software:

    Article Title: Rapid acquisition and model-based analysis of cell-free transcription–translation reactions from nonmodel bacteria
    Article Snippet: Calibration standards of purified GFP-MGapt mRNA, His6 -tagged GFP and mCherry were prepared in the standard reaction mixture (SI Appendix , Figs. S2 and S14 ). .. Full factorial experimental design and Echo525 liquid handler (Labcyte) pick list transfer instructions were automatically generated using a Python script (available at https://github.com/jmacdona/ODE_MCMC_tools ) or Labcyte PickList software. .. Liquid droplets were transferred as multiples of 25 nL to a final volume of 10 μL as three triplicate replicates.

    Article Title: The ace-1 Locus Is Amplified in All Resistant Anopheles gambiae Mosquitoes: Fitness Consequences of Homogeneous and Heterogeneous Duplications
    Article Snippet: We dispensed 0.5 μl of genomic DNA and 1.5 μl of reaction mixture containing specific primers, each at a concentration of 0.8 μM and 0.75 μl of Master Mix (LightCycler 480 SYBR Green I Master, Roche) into the wells of a 384-well plate, with a Labcyte Echo525 dispenser. .. Standard curves were constructed with 10-fold dilutions of a PCR product previously amplified with specific primers for each locus from KisumuP (SS) strain DNA.

    Article Title: Determination of equilibrium dissociation constants for recombinant antibodies by high-throughput affinity electrophoresis
    Article Snippet: The Labcyte Echo® 525 liquid handler (Echo) was used to perform the ADE sample transfer to the sample reservoirs on the EMSA cards. .. The Labcyte Echo® 525 liquid handler (Echo) was used to perform the ADE sample transfer to the sample reservoirs on the EMSA cards.

    Article Title: Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae, et al. Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae
    Article Snippet: We used the qPCR amplification conditions described by Assogba et al. ( ): 0.5 μl of genomic DNA and 1.5 μl of reaction mix containing 0.8 μM of each specific primer and 0.75 μl of mastermix (LightCycler® 480 SYBR Green I Master, Roche) were dispensed on a 384‐well plate using the Labcyte® Echo525 dispenser. .. We used the qPCR amplification conditions described by Assogba et al. ( ): 0.5 μl of genomic DNA and 1.5 μl of reaction mix containing 0.8 μM of each specific primer and 0.75 μl of mastermix (LightCycler® 480 SYBR Green I Master, Roche) were dispensed on a 384‐well plate using the Labcyte® Echo525 dispenser.

    Article Title: Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?
    Article Snippet: The qPCR assay was prepared in Roche 384 well PCR plates (product no. 04729749001) in a 10 μl reaction volume fired using an ECHO 525 non-contact micro dispenser (LabCyte). .. The qPCR assay was prepared in Roche 384 well PCR plates (product no. 04729749001) in a 10 μl reaction volume fired using an ECHO 525 non-contact micro dispenser (LabCyte).

    Real-time Polymerase Chain Reaction:

    Article Title: Salinity stress from the perspective of the energy-redox axis: Lessons from a marine intertidal flatworm
    Article Snippet: Using an Echo® 525 liquid handling system (Labcyte Inc., California, USA), 0.75 µl of LightCycler-FastStart DNA Master SYBR-Green I™ Mix (Roche, Mannheim, Germany), 0.015 µl of each primer (forward and reverse at 0.2 µM final concentration), 0.22 µl of ultra-pure water and 0.5 µl of cDNA were dispensed into a 384-well reaction plate. .. The qPCR conditions were as follows: denaturation at 95 °C for 10 min, followed by 45 cycles of repeat amplification (95 °C, 15 s), hybridization (60 °C or 62 °C according to the primer pair used, 5 s) and elongation (72 °C, 10 s) and a final step at 40 °C for 30 s. A melting curve program was performed to control the amplification specificity.

    Article Title: The ace-1 Locus Is Amplified in All Resistant Anopheles gambiae Mosquitoes: Fitness Consequences of Homogeneous and Heterogeneous Duplications
    Article Snippet: These flanking loci were used as qPCR markers of the amplified zone. .. We dispensed 0.5 μl of genomic DNA and 1.5 μl of reaction mixture containing specific primers, each at a concentration of 0.8 μM and 0.75 μl of Master Mix (LightCycler 480 SYBR Green I Master, Roche) into the wells of a 384-well plate, with a Labcyte Echo525 dispenser.

    Article Title: Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae, et al. Adaptive deletion in resistance gene duplications in the malaria vector Anopheles gambiae
    Article Snippet: We used the primer pair AgAce1qtidir2 and AgAce1qtirev2 primers to amplify a 185‐bp fragment of the ace‐1 gene (Table ), and the primer pair Del97Qdir5 and Del97Qrev4 to amplify a 186‐bp fragment overlapping the ID (Table ). .. We used the qPCR amplification conditions described by Assogba et al. ( ): 0.5 μl of genomic DNA and 1.5 μl of reaction mix containing 0.8 μM of each specific primer and 0.75 μl of mastermix (LightCycler® 480 SYBR Green I Master, Roche) were dispensed on a 384‐well plate using the Labcyte® Echo525 dispenser. .. The qPCR was performed with a 95°C activation step for 8 min followed by 45 cycles of 95°C for 4‐s, 67°C for 13 s, and 72°C for 19 s. Melting curves were generated by a postamplification melting step between 70°C and 95°C, for Tm analysis.

    Article Title: Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?
    Article Snippet: 7 μl of RNA at a concentration of 5 ng/μl (quantified using the Qubit 2.0 fluorometer) was used to generate cDNA in a 10 μl total extraction volume using the SuperScript VILO cDNA synthesis kit (Life Technologies, Catalogue Number 11754-050) followed by incubation at 25 °C for 10 min, 42 °C for 60 min and 85 °C for 5 min. .. The qPCR assay was prepared in Roche 384 well PCR plates (product no. 04729749001) in a 10 μl reaction volume fired using an ECHO 525 non-contact micro dispenser (LabCyte). .. Three primer probe pairs were used in each assay against the following housekeeping genes: RPLP0 (CY5), RPL19 (FAM) and ACTB (CYAN500) using three unique fluorophores compatible with the Roche LightCycler480 PCR machine.

    Multiplex Assay:

    Article Title: Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?
    Article Snippet: Multiplex quantitative 2-step reverse transcriptase PCR was performed to assess mRNA quality for the same sample set analysed on the Agilent. .. The qPCR assay was prepared in Roche 384 well PCR plates (product no. 04729749001) in a 10 μl reaction volume fired using an ECHO 525 non-contact micro dispenser (LabCyte).

    Concentration Assay:

    Article Title: Salinity stress from the perspective of the energy-redox axis: Lessons from a marine intertidal flatworm
    Article Snippet: Reverse transcription was carried out using M-MLV Reverse Transcriptase (Invitrogen, France). .. Using an Echo® 525 liquid handling system (Labcyte Inc., California, USA), 0.75 µl of LightCycler-FastStart DNA Master SYBR-Green I™ Mix (Roche, Mannheim, Germany), 0.015 µl of each primer (forward and reverse at 0.2 µM final concentration), 0.22 µl of ultra-pure water and 0.5 µl of cDNA were dispensed into a 384-well reaction plate. .. The qPCR conditions were as follows: denaturation at 95 °C for 10 min, followed by 45 cycles of repeat amplification (95 °C, 15 s), hybridization (60 °C or 62 °C according to the primer pair used, 5 s) and elongation (72 °C, 10 s) and a final step at 40 °C for 30 s. A melting curve program was performed to control the amplification specificity.

    Article Title: The ace-1 Locus Is Amplified in All Resistant Anopheles gambiae Mosquitoes: Fitness Consequences of Homogeneous and Heterogeneous Duplications
    Article Snippet: As they are located outside the amplicon, they should not be amplified. .. We dispensed 0.5 μl of genomic DNA and 1.5 μl of reaction mixture containing specific primers, each at a concentration of 0.8 μM and 0.75 μl of Master Mix (LightCycler 480 SYBR Green I Master, Roche) into the wells of a 384-well plate, with a Labcyte Echo525 dispenser. .. We performed qPCR as follows: activation at 95°C for 8 min, followed by 45 cycles of 95°C for 4 s, 67°C for 13 s, and 72°C for 19 s. Melting curves were generated by a post-amplification melting step between 70°C and 95°C, for Tm analysis.

    Article Title: Nucleic acid extraction from formalin-fixed paraffin-embedded cancer cell line samples: a trade off between quantity and quality?
    Article Snippet: 7 μl of RNA at a concentration of 5 ng/μl (quantified using the Qubit 2.0 fluorometer) was used to generate cDNA in a 10 μl total extraction volume using the SuperScript VILO cDNA synthesis kit (Life Technologies, Catalogue Number 11754-050) followed by incubation at 25 °C for 10 min, 42 °C for 60 min and 85 °C for 5 min. .. The qPCR assay was prepared in Roche 384 well PCR plates (product no. 04729749001) in a 10 μl reaction volume fired using an ECHO 525 non-contact micro dispenser (LabCyte).

    Lysis:

    Article Title: A novel prospective isolation of murine fetal liver progenitors to study in utero hematopoietic defects
    Article Snippet: Single P2-hCD4 -/+ MEPs and CMPs were sorted into 384-well plates containing lysis buffer and snap frozen. .. Dual indexed sequencing libraries were prepared from 0.1ng cDNA using an Echo525 automation system (Labcyte) in miniaturized reaction volumes.

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    Labcyte echo525 instrument
    Echo525 Instrument, supplied by Labcyte, used in various techniques. Bioz Stars score: 84/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/echo525 instrument/product/Labcyte
    Average 84 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    echo525 instrument - by Bioz Stars, 2019-10
    84/100 stars
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