echo 525 acoustic liquid handler  (Labcyte)


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    Name:
    Echo 525 Liquid Handler
    Description:
    The newest member of the Echo product family enables rapid higher volume transfer of protein based and biochemical reagents With a 25 nL increment and rapid transfer rates the Echo 525 Liquid Handler transfers reagents contamination free without sacrificing reagent volume or reagent quality to tubing tips or nozzles
    Catalog Number:
    001-10080
    Price:
    None
    Category:
    Liquid handler
    Quantity:
    1
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    Structured Review

    Labcyte echo 525 acoustic liquid handler
    Pooling by iSeq correlates with pooling by NovaSeq. 265 libraries were pooled at equal volumes (0.5uL per library) using the <t>Echo</t> 525 and sequenced on the Illumina iSeq to a total combined depth of approximately 4.5 million reads (standard deviation indicated by green dashed lines). Resultant reads were assigned to each barcoded library to calculate the percent of total reads occupied by each sample. These ratios were used to re-pool each library in appropriate volumes (ranging from 160nL to 3800nL) using the Echo 525 to achieve equal read representation across all 265 samples. Libraries pooled at equal volumes and sequenced on the iSeq occupied a mean of 0.378% ± 0.125% of total sequencing reads (standard deviation indicated by purple dashed lines). Re-pooled based on iSeq ratios, libraries sequenced on the Illumina NovaSeq demonstrated a significantly tighter standard deviation ( F test p
    The newest member of the Echo product family enables rapid higher volume transfer of protein based and biochemical reagents With a 25 nL increment and rapid transfer rates the Echo 525 Liquid Handler transfers reagents contamination free without sacrificing reagent volume or reagent quality to tubing tips or nozzles
    https://www.bioz.com/result/echo 525 acoustic liquid handler/product/Labcyte
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    echo 525 acoustic liquid handler - by Bioz Stars, 2020-05
    95/100 stars

    Images

    1) Product Images from "Miniaturization and optimization of 384-well compatible RNA sequencing library preparation"

    Article Title: Miniaturization and optimization of 384-well compatible RNA sequencing library preparation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0206194

    Pooling by iSeq correlates with pooling by NovaSeq. 265 libraries were pooled at equal volumes (0.5uL per library) using the Echo 525 and sequenced on the Illumina iSeq to a total combined depth of approximately 4.5 million reads (standard deviation indicated by green dashed lines). Resultant reads were assigned to each barcoded library to calculate the percent of total reads occupied by each sample. These ratios were used to re-pool each library in appropriate volumes (ranging from 160nL to 3800nL) using the Echo 525 to achieve equal read representation across all 265 samples. Libraries pooled at equal volumes and sequenced on the iSeq occupied a mean of 0.378% ± 0.125% of total sequencing reads (standard deviation indicated by purple dashed lines). Re-pooled based on iSeq ratios, libraries sequenced on the Illumina NovaSeq demonstrated a significantly tighter standard deviation ( F test p
    Figure Legend Snippet: Pooling by iSeq correlates with pooling by NovaSeq. 265 libraries were pooled at equal volumes (0.5uL per library) using the Echo 525 and sequenced on the Illumina iSeq to a total combined depth of approximately 4.5 million reads (standard deviation indicated by green dashed lines). Resultant reads were assigned to each barcoded library to calculate the percent of total reads occupied by each sample. These ratios were used to re-pool each library in appropriate volumes (ranging from 160nL to 3800nL) using the Echo 525 to achieve equal read representation across all 265 samples. Libraries pooled at equal volumes and sequenced on the iSeq occupied a mean of 0.378% ± 0.125% of total sequencing reads (standard deviation indicated by purple dashed lines). Re-pooled based on iSeq ratios, libraries sequenced on the Illumina NovaSeq demonstrated a significantly tighter standard deviation ( F test p

    Techniques Used: Standard Deviation, Sequencing

    Related Articles

    Sequencing:

    Article Title: Miniaturization and optimization of 384-well compatible RNA sequencing library preparation
    Article Snippet: .. Here, we describe the integration of several instruments, including the Labcyte Echo 525 acoustic liquid handler and the iSeq and NovaSeq Illumina sequencing platforms, to miniaturize and automate mNGS library preparation, significantly reducing the cost and the time required to prepare samples. ..

    High Throughput Screening Assay:

    Article Title: Selective Inhibition of FOXO1 Activator/Repressor Balance Modulates Hepatic Glucose Handling
    Article Snippet: .. Compound plates for the high-throughput screen were prepared using an Echo 525 Acoustic liquid handler (Labcyte) to dispense 100nl compound or DMSO from Echo-compatible 384-well source plates (Labcyte P5525) to luminescence-compatible 384-well plates (Greiner 481080). .. To test compounds in the reporter gene assay, cryopreserved transfected cells were thawed and diluted in low-glucose (1 g/l) DMEM (Invitrogen 11880-028) supplemented with GlutaMax (Invitrogen 35050-038), non-essential amino acids (Invitrogen 11140-035), 25 mM HEPES (Invitrogen 15630-056), 1% charcoal/dextran-treated FBS (Nordic Biolabs SH30068).

    Generated:

    Article Title: Rapid acquisition and model-based analysis of cell-free transcription–translation reactions from nonmodel bacteria
    Article Snippet: .. Full factorial experimental design and Echo525 liquid handler (Labcyte) pick list transfer instructions were automatically generated using a Python script (available at https://github.com/jmacdona/ODE_MCMC_tools ) or Labcyte PickList software. ..

    Article Title: Machine-driven parameter screen of biochemical reactions.
    Article Snippet: .. The development of complex methods in molecular biology is a laborious, costly, iterative and often intuition-bound process where optima are sought in a multidimensional parameter space through step-by-step optimizations. .. The development of complex methods in molecular biology is a laborious, costly, iterative and often intuition-bound process where optima are sought in a multidimensional parameter space through step-by-step optimizations.

    other:

    Article Title: Determination of equilibrium dissociation constants for recombinant antibodies by high-throughput affinity electrophoresis
    Article Snippet: EMSA card and ADE integration The Labcyte Echo® 525 liquid handler (Echo) was used to perform the ADE sample transfer to the sample reservoirs on the EMSA cards.

    Software:

    Article Title: Rapid acquisition and model-based analysis of cell-free transcription–translation reactions from nonmodel bacteria
    Article Snippet: .. Full factorial experimental design and Echo525 liquid handler (Labcyte) pick list transfer instructions were automatically generated using a Python script (available at https://github.com/jmacdona/ODE_MCMC_tools ) or Labcyte PickList software. ..

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  • 95
    Labcyte echo 525 acoustic liquid handler
    Pooling by iSeq correlates with pooling by NovaSeq. 265 libraries were pooled at equal volumes (0.5uL per library) using the <t>Echo</t> 525 and sequenced on the Illumina iSeq to a total combined depth of approximately 4.5 million reads (standard deviation indicated by green dashed lines). Resultant reads were assigned to each barcoded library to calculate the percent of total reads occupied by each sample. These ratios were used to re-pool each library in appropriate volumes (ranging from 160nL to 3800nL) using the Echo 525 to achieve equal read representation across all 265 samples. Libraries pooled at equal volumes and sequenced on the iSeq occupied a mean of 0.378% ± 0.125% of total sequencing reads (standard deviation indicated by purple dashed lines). Re-pooled based on iSeq ratios, libraries sequenced on the Illumina NovaSeq demonstrated a significantly tighter standard deviation ( F test p
    Echo 525 Acoustic Liquid Handler, supplied by Labcyte, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/echo 525 acoustic liquid handler/product/Labcyte
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    echo 525 acoustic liquid handler - by Bioz Stars, 2020-05
    95/100 stars
      Buy from Supplier

    Image Search Results


    Pooling by iSeq correlates with pooling by NovaSeq. 265 libraries were pooled at equal volumes (0.5uL per library) using the Echo 525 and sequenced on the Illumina iSeq to a total combined depth of approximately 4.5 million reads (standard deviation indicated by green dashed lines). Resultant reads were assigned to each barcoded library to calculate the percent of total reads occupied by each sample. These ratios were used to re-pool each library in appropriate volumes (ranging from 160nL to 3800nL) using the Echo 525 to achieve equal read representation across all 265 samples. Libraries pooled at equal volumes and sequenced on the iSeq occupied a mean of 0.378% ± 0.125% of total sequencing reads (standard deviation indicated by purple dashed lines). Re-pooled based on iSeq ratios, libraries sequenced on the Illumina NovaSeq demonstrated a significantly tighter standard deviation ( F test p

    Journal: PLoS ONE

    Article Title: Miniaturization and optimization of 384-well compatible RNA sequencing library preparation

    doi: 10.1371/journal.pone.0206194

    Figure Lengend Snippet: Pooling by iSeq correlates with pooling by NovaSeq. 265 libraries were pooled at equal volumes (0.5uL per library) using the Echo 525 and sequenced on the Illumina iSeq to a total combined depth of approximately 4.5 million reads (standard deviation indicated by green dashed lines). Resultant reads were assigned to each barcoded library to calculate the percent of total reads occupied by each sample. These ratios were used to re-pool each library in appropriate volumes (ranging from 160nL to 3800nL) using the Echo 525 to achieve equal read representation across all 265 samples. Libraries pooled at equal volumes and sequenced on the iSeq occupied a mean of 0.378% ± 0.125% of total sequencing reads (standard deviation indicated by purple dashed lines). Re-pooled based on iSeq ratios, libraries sequenced on the Illumina NovaSeq demonstrated a significantly tighter standard deviation ( F test p

    Article Snippet: Here, we describe the integration of several instruments, including the Labcyte Echo 525 acoustic liquid handler and the iSeq and NovaSeq Illumina sequencing platforms, to miniaturize and automate mNGS library preparation, significantly reducing the cost and the time required to prepare samples.

    Techniques: Standard Deviation, Sequencing