β actin 13e5  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β actin 13e5
    β Actin 13e5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    β actin 13e5 rabbit mab hrp conjugate  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β actin 13e5 rabbit mab hrp conjugate
    β Actin 13e5 Rabbit Mab Hrp Conjugate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    β actin 13e5 rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β actin 13e5 rabbit mab

    β Actin 13e5 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "m 6 A demethylase ALKBH5 attenuates doxorubicin-induced cardiotoxicity via posttranscriptional stabilization of Rasal3"

    Article Title: m 6 A demethylase ALKBH5 attenuates doxorubicin-induced cardiotoxicity via posttranscriptional stabilization of Rasal3

    Journal: iScience

    doi: 10.1016/j.isci.2023.106215


    Figure Legend Snippet:

    Techniques Used: Negative Control, Recombinant, Staining, TUNEL Assay, Enzyme-linked Immunosorbent Assay, Isolation, ATP Assay, Activation Assay, Software

    anti β actin 13e5 rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti β actin 13e5 rabbit mab
    Anti β Actin 13e5 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti β actin 13e5 rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti β actin 13e5 rabbit mab
    Anti β Actin 13e5 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    β actin 13e5  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β actin 13e5
    β Actin 13e5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti β actin 13e5  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti β actin 13e5
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    β actin 13e5 rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β actin 13e5 rabbit mab
    Workflow to analyze the genotype-phenotype relation in RASless mouse embryonic fibroblast (MEF) cell lines and phenotypic data in MEF cells (A) Schematic workflow of MEF cell lines with different genetic backgrounds analyzed in this work. (B) KRAS mutant protein levels obtained by Western blot analysis of lysates of RASless MEF cell lines expressing KRAS WT, different oncogenic KRAS mutants, or BRAF V600E (as negative control for KRAS expression). To obtain nine biological replicates, MEF cell lines were grown for three consecutive weeks with similar cell densities before cell lysis at three different times of the day: 9 a.m., 12 p.m., and 3 p.m.. The western blots show three biological replicates. The bar graph shows the results of nine biological replicates for the abundance of KRAS, normalized by <t>β-actin</t> as loading control. (C) Results of cell migration phenotypes. Representative images of MEF cell lines grown on RadiusTM plates at different time points. (D) The bar graph shows the open wound areas for MEF cell lines at 12 h (three replicates). (E) Power of hydrogen (pH) analysis of MEF cell line growth media. Representative images of MEF culture dishes after 14 days in culture for WT, G12D, G12V, and Q61L MEF cell lines. (F) The bar graph below shows the mean and SEM values of OD 415/560 ratios (triplicates) for MEF cell lines at time 0, 25, 72, 168, and 216 h (each with three biological replicates). The insert represents, for each cell line, the overall least square (LS) mean of all values. (G) Cell proliferation analyzed using the Scepter 2.0 Automated Cell Counter. Time course of viable cells (n = 3). The insert represents, for each cell line, the overall LS of the mean of the proliferation rate calculated between 0 and 72 h. (H) Cell proliferation analyzed using the CyQUANT Assay. Time course of DNA concentration (n = 3). The insert represents, for each cell line, the overall LS of the mean of the proliferation rate calculated between 0 and 72 h. (I) Cell proliferation analyzed using the Pierce™ BCA Protein Assay. Time course of protein concentration (n = 3). The insert represents, for each cell line, the overall LS of the mean of the proliferation rate calculated between 0 and 72 h. (J) Cell viability analyzed using the CellTiter-Blue Assay. Time course of fluorescence intensity (n = 3). The insert represents, for each cell line, the overall LS of the mean of the viability rate calculated between 0 and 72 h. (K) Cell viability analyzed using the CellTiter-Glo Assay. Time course of ATP concentration (n = 3). The insert represents, for each cell line, the overall LS of the mean of the viability rate calculated between 0 and 72 h. (L) Bioenergetic profile measured using the Real-Time ATP-rate assay with a Seahorse XF-analyzer. Total ATP production rate, which is the sum of ATP production through oxidative phosphorylation (blue) and glycolysis (red), was measured for each MEF cell line at 2 cell density and normalized by the protein content of each well measured by Pierce 660 nm Protein Assay (n = 7–8 per group). The insert represents for each cell line, the overall LS mean of the total ATP production rate normalized by the protein content. All statistical analyses displayed are the main effect “cell line” of a two-way ANOVA followed by Tukey’s post-hoc test. Means not sharing any letter are significantly different by the Tukey-test at the 5% level of significance. For example, means labeled by “a” are statistically significantly different from means labeled by “b” (and “c, etc …) but not from means labeled by “ab”.
    β Actin 13e5 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β actin 13e5 rabbit mab/product/Cell Signaling Technology Inc
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    Images

    1) Product Images from "Whole-cell energy modeling reveals quantitative changes of predicted energy flows in RAS mutant cancer cell lines"

    Article Title: Whole-cell energy modeling reveals quantitative changes of predicted energy flows in RAS mutant cancer cell lines

    Journal: iScience

    doi: 10.1016/j.isci.2023.105931

    Workflow to analyze the genotype-phenotype relation in RASless mouse embryonic fibroblast (MEF) cell lines and phenotypic data in MEF cells (A) Schematic workflow of MEF cell lines with different genetic backgrounds analyzed in this work. (B) KRAS mutant protein levels obtained by Western blot analysis of lysates of RASless MEF cell lines expressing KRAS WT, different oncogenic KRAS mutants, or BRAF V600E (as negative control for KRAS expression). To obtain nine biological replicates, MEF cell lines were grown for three consecutive weeks with similar cell densities before cell lysis at three different times of the day: 9 a.m., 12 p.m., and 3 p.m.. The western blots show three biological replicates. The bar graph shows the results of nine biological replicates for the abundance of KRAS, normalized by β-actin as loading control. (C) Results of cell migration phenotypes. Representative images of MEF cell lines grown on RadiusTM plates at different time points. (D) The bar graph shows the open wound areas for MEF cell lines at 12 h (three replicates). (E) Power of hydrogen (pH) analysis of MEF cell line growth media. Representative images of MEF culture dishes after 14 days in culture for WT, G12D, G12V, and Q61L MEF cell lines. (F) The bar graph below shows the mean and SEM values of OD 415/560 ratios (triplicates) for MEF cell lines at time 0, 25, 72, 168, and 216 h (each with three biological replicates). The insert represents, for each cell line, the overall least square (LS) mean of all values. (G) Cell proliferation analyzed using the Scepter 2.0 Automated Cell Counter. Time course of viable cells (n = 3). The insert represents, for each cell line, the overall LS of the mean of the proliferation rate calculated between 0 and 72 h. (H) Cell proliferation analyzed using the CyQUANT Assay. Time course of DNA concentration (n = 3). The insert represents, for each cell line, the overall LS of the mean of the proliferation rate calculated between 0 and 72 h. (I) Cell proliferation analyzed using the Pierce™ BCA Protein Assay. Time course of protein concentration (n = 3). The insert represents, for each cell line, the overall LS of the mean of the proliferation rate calculated between 0 and 72 h. (J) Cell viability analyzed using the CellTiter-Blue Assay. Time course of fluorescence intensity (n = 3). The insert represents, for each cell line, the overall LS of the mean of the viability rate calculated between 0 and 72 h. (K) Cell viability analyzed using the CellTiter-Glo Assay. Time course of ATP concentration (n = 3). The insert represents, for each cell line, the overall LS of the mean of the viability rate calculated between 0 and 72 h. (L) Bioenergetic profile measured using the Real-Time ATP-rate assay with a Seahorse XF-analyzer. Total ATP production rate, which is the sum of ATP production through oxidative phosphorylation (blue) and glycolysis (red), was measured for each MEF cell line at 2 cell density and normalized by the protein content of each well measured by Pierce 660 nm Protein Assay (n = 7–8 per group). The insert represents for each cell line, the overall LS mean of the total ATP production rate normalized by the protein content. All statistical analyses displayed are the main effect “cell line” of a two-way ANOVA followed by Tukey’s post-hoc test. Means not sharing any letter are significantly different by the Tukey-test at the 5% level of significance. For example, means labeled by “a” are statistically significantly different from means labeled by “b” (and “c, etc …) but not from means labeled by “ab”.
    Figure Legend Snippet: Workflow to analyze the genotype-phenotype relation in RASless mouse embryonic fibroblast (MEF) cell lines and phenotypic data in MEF cells (A) Schematic workflow of MEF cell lines with different genetic backgrounds analyzed in this work. (B) KRAS mutant protein levels obtained by Western blot analysis of lysates of RASless MEF cell lines expressing KRAS WT, different oncogenic KRAS mutants, or BRAF V600E (as negative control for KRAS expression). To obtain nine biological replicates, MEF cell lines were grown for three consecutive weeks with similar cell densities before cell lysis at three different times of the day: 9 a.m., 12 p.m., and 3 p.m.. The western blots show three biological replicates. The bar graph shows the results of nine biological replicates for the abundance of KRAS, normalized by β-actin as loading control. (C) Results of cell migration phenotypes. Representative images of MEF cell lines grown on RadiusTM plates at different time points. (D) The bar graph shows the open wound areas for MEF cell lines at 12 h (three replicates). (E) Power of hydrogen (pH) analysis of MEF cell line growth media. Representative images of MEF culture dishes after 14 days in culture for WT, G12D, G12V, and Q61L MEF cell lines. (F) The bar graph below shows the mean and SEM values of OD 415/560 ratios (triplicates) for MEF cell lines at time 0, 25, 72, 168, and 216 h (each with three biological replicates). The insert represents, for each cell line, the overall least square (LS) mean of all values. (G) Cell proliferation analyzed using the Scepter 2.0 Automated Cell Counter. Time course of viable cells (n = 3). The insert represents, for each cell line, the overall LS of the mean of the proliferation rate calculated between 0 and 72 h. (H) Cell proliferation analyzed using the CyQUANT Assay. Time course of DNA concentration (n = 3). The insert represents, for each cell line, the overall LS of the mean of the proliferation rate calculated between 0 and 72 h. (I) Cell proliferation analyzed using the Pierce™ BCA Protein Assay. Time course of protein concentration (n = 3). The insert represents, for each cell line, the overall LS of the mean of the proliferation rate calculated between 0 and 72 h. (J) Cell viability analyzed using the CellTiter-Blue Assay. Time course of fluorescence intensity (n = 3). The insert represents, for each cell line, the overall LS of the mean of the viability rate calculated between 0 and 72 h. (K) Cell viability analyzed using the CellTiter-Glo Assay. Time course of ATP concentration (n = 3). The insert represents, for each cell line, the overall LS of the mean of the viability rate calculated between 0 and 72 h. (L) Bioenergetic profile measured using the Real-Time ATP-rate assay with a Seahorse XF-analyzer. Total ATP production rate, which is the sum of ATP production through oxidative phosphorylation (blue) and glycolysis (red), was measured for each MEF cell line at 2 cell density and normalized by the protein content of each well measured by Pierce 660 nm Protein Assay (n = 7–8 per group). The insert represents for each cell line, the overall LS mean of the total ATP production rate normalized by the protein content. All statistical analyses displayed are the main effect “cell line” of a two-way ANOVA followed by Tukey’s post-hoc test. Means not sharing any letter are significantly different by the Tukey-test at the 5% level of significance. For example, means labeled by “a” are statistically significantly different from means labeled by “b” (and “c, etc …) but not from means labeled by “ab”.

    Techniques Used: Mutagenesis, Western Blot, Expressing, Negative Control, Lysis, Migration, CyQUANT Assay, Concentration Assay, Bicinchoninic Acid Protein Assay, Protein Concentration, CtB Assay, Fluorescence, Glo Assay, Labeling


    Figure Legend Snippet:

    Techniques Used: Recombinant, Cell Migration Assay, CyQUANT Assay, Bicinchoninic Acid Protein Assay, Viability Assay, Cell Viability Assay, Mass Spectrometry, Expressing, Transduction, Software

    β actin 13e5  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β actin 13e5
    β Actin 13e5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    β actin 13e5  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β actin 13e5

    β Actin 13e5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β actin 13e5/product/Cell Signaling Technology Inc
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    1) Product Images from "Immune activation of characteristic gut mycobiota Kazachstania pintolopesii on IL-23/IL-17R signaling in ankylosing spondylitis"

    Article Title: Immune activation of characteristic gut mycobiota Kazachstania pintolopesii on IL-23/IL-17R signaling in ankylosing spondylitis

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2022.1035366


    Figure Legend Snippet:

    Techniques Used:

    β actin 13e5 rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β actin 13e5 rabbit mab
    KEY RESOURCES TABLE
    β Actin 13e5 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β actin 13e5 rabbit mab/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    β actin 13e5 rabbit mab - by Bioz Stars, 2023-03
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    1) Product Images from "Crosstalk between pro-survival sphingolipid metabolism and complement signaling induces inflammasome-mediated tumor metastasis"

    Article Title: Crosstalk between pro-survival sphingolipid metabolism and complement signaling induces inflammasome-mediated tumor metastasis

    Journal: Cell reports

    doi: 10.1016/j.celrep.2022.111742

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Wound Healing Assay, Invasion Assay, MTT Cell Proliferation, Protease Inhibitor, Western Blot, Lysis, In Situ Hybridization, Plasmid Preparation, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, RNA Sequencing Assay, Expressing, Knock-Out, shRNA, CRISPR, Clone Assay, Software, Imaging

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    Image Search Results


    Journal: iScience

    Article Title: m 6 A demethylase ALKBH5 attenuates doxorubicin-induced cardiotoxicity via posttranscriptional stabilization of Rasal3

    doi: 10.1016/j.isci.2023.106215

    Figure Lengend Snippet:

    Article Snippet: β-actin (13E5) Rabbit mAb , CST , Cat#4970S.

    Techniques: Negative Control, Recombinant, Staining, TUNEL Assay, Enzyme-linked Immunosorbent Assay, Isolation, ATP Assay, Activation Assay, Software