Journal: Life

Article Title: Immunoreactivity of Muscarinic Acetylcholine M2 and Serotonin 5-HT2B Receptors, Norepinephrine Transporter and Kir Channels in a Model of Epilepsy

doi: 10.3390/life11040276

Figure Lengend Snippet: Representative images of immunoreactivity for serotonin receptors within the cortex, hippocampus, and medulla in control ( A – C , respectively) and PTZ-kindled ( D – F , respectively). Figure ( G ) represents the immunoreactivity of the serotonin receptor 2B in several regions (cortex, medulla, and hippocampus). Values are presented as the mean ± SEM, n = 10, * p < 0.05, and *** p < 0.001. The black arrow indicates the immunoreactive cells. Pictures were taken at a magnification of ×200. Scale bar: 50 µm.

Article Snippet: Primary antibodies against Kir3.1 (mouse monoclonal, 1:200, Alomone Labs, Jerusalem, Israel (APC-005)), Kir6.2 (rabbit polyclonal, 1:200, Alomone Labs, Jerusalem, Israel (APC-020)), muscarinic acetylcholine receptor M2, mAChR (rabbit polyclonal, 1:200, Alomone Labs, Jerusalem, Israel (APC-002)), norepinephrine transporter (rabbit polyclonal, 1:200, Alomone Labs, Jerusalem, Israel (AMT-002)) and serotonin receptor 2B (rabbit polyclonal, 1:200, Alomone Labs, Jerusalem, Israel (ASR-035)) were diluted in PBS and incubated with the slides at 4 °C.

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