α p53bp1 t543  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc α p53bp1 t543
    ( a ) Representative images of immunofluorescence showing RPA2 foci (AlexaFluor594-Red) in RAW264.7 macrophages infected with GFP expressing Rv, Ra (Green) at 24 h p.i. ( b and c ) We assessed the activation of various DDR proteins activated in response to DSBs mediated through Rv infection in THP-1 ( b ) and RAW264.7 ( c ) cells. WCL prepared at indicated h.p.i. were subjected to immunoblotting with α-γH2AX, α-pATM(S1981), α-pCHK2(T68), <t>α-p53BP1-T543,</t> α-MRE11, α-pNbs1-S343, α−53BP1, α-pMDC1, α-pMDC1-T4 and α-β-actin antibodies.
    α P53bp1 T543, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α p53bp1 t543/product/Cell Signaling Technology Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α p53bp1 t543 - by Bioz Stars, 2023-03
    92/100 stars

    Images

    1) Product Images from "Mycobacterium tuberculosis exploits host ATM kinase for survival advantage through SecA2 secretome"

    Article Title: Mycobacterium tuberculosis exploits host ATM kinase for survival advantage through SecA2 secretome

    Journal: eLife

    doi: 10.7554/eLife.51466

    ( a ) Representative images of immunofluorescence showing RPA2 foci (AlexaFluor594-Red) in RAW264.7 macrophages infected with GFP expressing Rv, Ra (Green) at 24 h p.i. ( b and c ) We assessed the activation of various DDR proteins activated in response to DSBs mediated through Rv infection in THP-1 ( b ) and RAW264.7 ( c ) cells. WCL prepared at indicated h.p.i. were subjected to immunoblotting with α-γH2AX, α-pATM(S1981), α-pCHK2(T68), α-p53BP1-T543, α-MRE11, α-pNbs1-S343, α−53BP1, α-pMDC1, α-pMDC1-T4 and α-β-actin antibodies.
    Figure Legend Snippet: ( a ) Representative images of immunofluorescence showing RPA2 foci (AlexaFluor594-Red) in RAW264.7 macrophages infected with GFP expressing Rv, Ra (Green) at 24 h p.i. ( b and c ) We assessed the activation of various DDR proteins activated in response to DSBs mediated through Rv infection in THP-1 ( b ) and RAW264.7 ( c ) cells. WCL prepared at indicated h.p.i. were subjected to immunoblotting with α-γH2AX, α-pATM(S1981), α-pCHK2(T68), α-p53BP1-T543, α-MRE11, α-pNbs1-S343, α−53BP1, α-pMDC1, α-pMDC1-T4 and α-β-actin antibodies.

    Techniques Used: Immunofluorescence, Infection, Expressing, Activation Assay, Western Blot

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    • α p53bp1 t543  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc α p53bp1 t543
    ( a ) Representative images of immunofluorescence showing RPA2 foci (AlexaFluor594-Red) in RAW264.7 macrophages infected with GFP expressing Rv, Ra (Green) at 24 h p.i. ( b and c ) We assessed the activation of various DDR proteins activated in response to DSBs mediated through Rv infection in THP-1 ( b ) and RAW264.7 ( c ) cells. WCL prepared at indicated h.p.i. were subjected to immunoblotting with α-γH2AX, α-pATM(S1981), α-pCHK2(T68), <t>α-p53BP1-T543,</t> α-MRE11, α-pNbs1-S343, α−53BP1, α-pMDC1, α-pMDC1-T4 and α-β-actin antibodies.
    α P53bp1 T543, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α p53bp1 t543/product/Cell Signaling Technology Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α p53bp1 t543 - by Bioz Stars, 2023-03
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) Representative images of immunofluorescence showing RPA2 foci (AlexaFluor594-Red) in RAW264.7 macrophages infected with GFP expressing Rv, Ra (Green) at 24 h p.i. ( b and c ) We assessed the activation of various DDR proteins activated in response to DSBs mediated through Rv infection in THP-1 ( b ) and RAW264.7 ( c ) cells. WCL prepared at indicated h.p.i. were subjected to immunoblotting with α-γH2AX, α-pATM(S1981), α-pCHK2(T68), α-p53BP1-T543, α-MRE11, α-pNbs1-S343, α−53BP1, α-pMDC1, α-pMDC1-T4 and α-β-actin antibodies.

    Journal: eLife

    Article Title: Mycobacterium tuberculosis exploits host ATM kinase for survival advantage through SecA2 secretome

    doi: 10.7554/eLife.51466

    Figure Lengend Snippet: ( a ) Representative images of immunofluorescence showing RPA2 foci (AlexaFluor594-Red) in RAW264.7 macrophages infected with GFP expressing Rv, Ra (Green) at 24 h p.i. ( b and c ) We assessed the activation of various DDR proteins activated in response to DSBs mediated through Rv infection in THP-1 ( b ) and RAW264.7 ( c ) cells. WCL prepared at indicated h.p.i. were subjected to immunoblotting with α-γH2AX, α-pATM(S1981), α-pCHK2(T68), α-p53BP1-T543, α-MRE11, α-pNbs1-S343, α−53BP1, α-pMDC1, α-pMDC1-T4 and α-β-actin antibodies.

    Article Snippet: All cell culture reagents were procured from Thermo fisher scientific Inc or Hyclone. α-γH2AX (2577S), α-ATM (2873S), α-pATR-S428 (2853S), α-pChk1-S345 (2348S), α-pChk2-T68 (2661S), α-Chk2 (2662S), α-CHK1(2360), α-ATR (13934), α-pAkt-S473 (9271S), α-Akt (9272S), α-p53BP1-T543 (3428S), α-MRE11(4895S), α-pNbs1-S343 and α-Nbs1 (3002S) antibodies were procured from Cell Signalling Technologies. α-pATM-S1891(ab81292) α−53BP1 (ab36823), α-pMDC1-T4 (ab35967), α-RPA2 (ab2175) and α-p53 (ab28) antibody was procured from Abcam. α-p21 (sc-817), α-β-actin (sc-47778), α-β-tubulin (sc-55529) and α-GAPDH (sc-25778) antibodies were purchase from Santacruz Biotechnology. α-DNA-PKcs (SAB4502385), α-pDNA-PKcs-S2056 (SAB4504169) were purchased from Sigma. α-MDC1 and α-pRPA2-S4/S8 was purchased from Novus Biologicals and HRP conjugated secondary antibodies were purchased from Jackson laboratories. α-MDC1 was purchased from Novus Biologicals.

    Techniques: Immunofluorescence, Infection, Expressing, Activation Assay, Western Blot