ß lactamase enzyme activity  (ATCC)


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    ATCC ß lactamase enzyme activity
    Antibiotic-resistance mechanisms and virulence factors in S. aureus isolates. a Assessment of efflux pump activity in five antibiotic-resistant and five susceptible isolates. EtBr efflux assay was performed using 3 µg/mL of EtBr and 30 µg/mL of CPZ. The fluorescent intensity (530 nm/590 nm) was monitored for 60 min after reenergizing the bacterial cells to trigger EtBr efflux with glucose (0.4% v/v). b Assessment of <t>ß-lactamase</t> enzyme activity in the isolates. The isolates were subjected to a Nitrocefin assay where the absorbance of the cell-free extract mixed with Nitrocefin was detected at 490 nm for 15 min. c Distribution of hemolysin manifestation by the 43 isolates. d Alpha hemolysin manifestation by Sa30. Each isolate was cultured in TSA plates with 5% sheep blood for 24 h. The hemolysis was detected visually by the translucency around the bacterial colony. e Distribution of biofilm-forming ability by the 43 isolates. The biofilm formation was assessed using a crystal violet assay. All isolates were classified into weak, moderate, and strong biofilm-formers based on their biofilm-forming ability. f Fluorescence microscopic image of extracellular polymeric substances (EPS) and Sa30 cells. A green (505 nm) filter was used to acquire the GFP-labeled Sa30 biofilms using a high-content screening microscope, Cell Discoverer 7. Alphabets (in tabular data) indicate a significant difference ( p < 0.05). ‘N/A’ stands for not applicable. More data on the resistance mechanisms and virulence factors are provided in Tables S and S . The experiment was performed in quadruplicates and repeated thrice to ensure reproducibility
    ß Lactamase Enzyme Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Genomic and phenotypic profiling of Staphylococcus aureus isolates from bovine mastitis for antibiotic resistance and intestinal infectivity"

    Article Title: Genomic and phenotypic profiling of Staphylococcus aureus isolates from bovine mastitis for antibiotic resistance and intestinal infectivity

    Journal: BMC Microbiology

    doi: 10.1186/s12866-023-02785-1

    Antibiotic-resistance mechanisms and virulence factors in S. aureus isolates. a Assessment of efflux pump activity in five antibiotic-resistant and five susceptible isolates. EtBr efflux assay was performed using 3 µg/mL of EtBr and 30 µg/mL of CPZ. The fluorescent intensity (530 nm/590 nm) was monitored for 60 min after reenergizing the bacterial cells to trigger EtBr efflux with glucose (0.4% v/v). b Assessment of ß-lactamase enzyme activity in the isolates. The isolates were subjected to a Nitrocefin assay where the absorbance of the cell-free extract mixed with Nitrocefin was detected at 490 nm for 15 min. c Distribution of hemolysin manifestation by the 43 isolates. d Alpha hemolysin manifestation by Sa30. Each isolate was cultured in TSA plates with 5% sheep blood for 24 h. The hemolysis was detected visually by the translucency around the bacterial colony. e Distribution of biofilm-forming ability by the 43 isolates. The biofilm formation was assessed using a crystal violet assay. All isolates were classified into weak, moderate, and strong biofilm-formers based on their biofilm-forming ability. f Fluorescence microscopic image of extracellular polymeric substances (EPS) and Sa30 cells. A green (505 nm) filter was used to acquire the GFP-labeled Sa30 biofilms using a high-content screening microscope, Cell Discoverer 7. Alphabets (in tabular data) indicate a significant difference ( p < 0.05). ‘N/A’ stands for not applicable. More data on the resistance mechanisms and virulence factors are provided in Tables S and S . The experiment was performed in quadruplicates and repeated thrice to ensure reproducibility
    Figure Legend Snippet: Antibiotic-resistance mechanisms and virulence factors in S. aureus isolates. a Assessment of efflux pump activity in five antibiotic-resistant and five susceptible isolates. EtBr efflux assay was performed using 3 µg/mL of EtBr and 30 µg/mL of CPZ. The fluorescent intensity (530 nm/590 nm) was monitored for 60 min after reenergizing the bacterial cells to trigger EtBr efflux with glucose (0.4% v/v). b Assessment of ß-lactamase enzyme activity in the isolates. The isolates were subjected to a Nitrocefin assay where the absorbance of the cell-free extract mixed with Nitrocefin was detected at 490 nm for 15 min. c Distribution of hemolysin manifestation by the 43 isolates. d Alpha hemolysin manifestation by Sa30. Each isolate was cultured in TSA plates with 5% sheep blood for 24 h. The hemolysis was detected visually by the translucency around the bacterial colony. e Distribution of biofilm-forming ability by the 43 isolates. The biofilm formation was assessed using a crystal violet assay. All isolates were classified into weak, moderate, and strong biofilm-formers based on their biofilm-forming ability. f Fluorescence microscopic image of extracellular polymeric substances (EPS) and Sa30 cells. A green (505 nm) filter was used to acquire the GFP-labeled Sa30 biofilms using a high-content screening microscope, Cell Discoverer 7. Alphabets (in tabular data) indicate a significant difference ( p < 0.05). ‘N/A’ stands for not applicable. More data on the resistance mechanisms and virulence factors are provided in Tables S and S . The experiment was performed in quadruplicates and repeated thrice to ensure reproducibility

    Techniques Used: Activity Assay, Cell Culture, Crystal Violet Assay, Fluorescence, Labeling, High Content Screening, Microscopy

    β lactamase activity  (ATCC)


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    ATCC β lactamase activity
    β Lactamase Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    negative β lactamase activity  (ATCC)


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    ATCC negative β lactamase activity
    Negative β Lactamase Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    β lactamase activity  (ATCC)


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    ATCC β lactamase activity
    β Lactamase Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    beta lactamase activity  (ATCC)


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    ATCC beta lactamase activity
    Antimicrobial susceptibility and minimum inhibitory concentrations of L. plantarum ATCC 202195-A and L. plantarum ATCC 202195-B.
    Beta Lactamase Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Antimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium Lactiplantibacillus plantarum, strain ATCC 202195"

    Article Title: Antimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium Lactiplantibacillus plantarum, strain ATCC 202195

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-94997-6

    Antimicrobial susceptibility and minimum inhibitory concentrations of L. plantarum ATCC 202195-A and L. plantarum ATCC 202195-B.
    Figure Legend Snippet: Antimicrobial susceptibility and minimum inhibitory concentrations of L. plantarum ATCC 202195-A and L. plantarum ATCC 202195-B.

    Techniques Used: Diffusion-based Assay, Activity Assay

    beta lactamase activity  (ATCC)


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    ATCC beta lactamase activity
    Antimicrobial susceptibility and minimum inhibitory concentrations of L. plantarum ATCC 202195-A and L. plantarum ATCC 202195-B.
    Beta Lactamase Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Antimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium Lactiplantibacillus plantarum, strain ATCC 202195"

    Article Title: Antimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium Lactiplantibacillus plantarum, strain ATCC 202195

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-94997-6

    Antimicrobial susceptibility and minimum inhibitory concentrations of L. plantarum ATCC 202195-A and L. plantarum ATCC 202195-B.
    Figure Legend Snippet: Antimicrobial susceptibility and minimum inhibitory concentrations of L. plantarum ATCC 202195-A and L. plantarum ATCC 202195-B.

    Techniques Used: Diffusion-based Assay, Activity Assay

    beta lactamase activity  (ATCC)


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    ATCC beta lactamase activity
    Antibiotic screen reveals S. marcescens genes important for growth and survival. A) Screen schematic: An input library (containing about 2 million insertion mutants) is grown to OD 0.1 and divided between 4 conditions for an additional 6 hours of growth: LB alone; LB + cefoxitin 4 ug/mL; LB + cefepime 0.025 ug/mL; and LB + ciprofloxacin 0.05 ug/mL. B) Under screen conditions, the library without drug selection undergoes more than 2 log expansion while those with antibiotic decrease in CFU to similar extent (about 0.5 log), with the library in cefoxitin undergoes expansion after 4 hours, likely due to upregulation of AmpC <t>beta-lactamase.</t> Libraries are harvested for analysis at 6 hours. C) Number of genes with insertion-mutants showing ≥ 4-fold change at 6 hours, compared to outgrowth in LB alone. D) Venn diagram illustrating genes showing coordinate enrichment or depletion.
    Beta Lactamase Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A genome-scale antibiotic screen in Serratia marcescens identifies YdgH as a conserved modifier of cephalosporin and detergent susceptibility"

    Article Title: A genome-scale antibiotic screen in Serratia marcescens identifies YdgH as a conserved modifier of cephalosporin and detergent susceptibility

    Journal: bioRxiv

    doi: 10.1101/2021.04.16.440252

    Antibiotic screen reveals S. marcescens genes important for growth and survival. A) Screen schematic: An input library (containing about 2 million insertion mutants) is grown to OD 0.1 and divided between 4 conditions for an additional 6 hours of growth: LB alone; LB + cefoxitin 4 ug/mL; LB + cefepime 0.025 ug/mL; and LB + ciprofloxacin 0.05 ug/mL. B) Under screen conditions, the library without drug selection undergoes more than 2 log expansion while those with antibiotic decrease in CFU to similar extent (about 0.5 log), with the library in cefoxitin undergoes expansion after 4 hours, likely due to upregulation of AmpC beta-lactamase. Libraries are harvested for analysis at 6 hours. C) Number of genes with insertion-mutants showing ≥ 4-fold change at 6 hours, compared to outgrowth in LB alone. D) Venn diagram illustrating genes showing coordinate enrichment or depletion.
    Figure Legend Snippet: Antibiotic screen reveals S. marcescens genes important for growth and survival. A) Screen schematic: An input library (containing about 2 million insertion mutants) is grown to OD 0.1 and divided between 4 conditions for an additional 6 hours of growth: LB alone; LB + cefoxitin 4 ug/mL; LB + cefepime 0.025 ug/mL; and LB + ciprofloxacin 0.05 ug/mL. B) Under screen conditions, the library without drug selection undergoes more than 2 log expansion while those with antibiotic decrease in CFU to similar extent (about 0.5 log), with the library in cefoxitin undergoes expansion after 4 hours, likely due to upregulation of AmpC beta-lactamase. Libraries are harvested for analysis at 6 hours. C) Number of genes with insertion-mutants showing ≥ 4-fold change at 6 hours, compared to outgrowth in LB alone. D) Venn diagram illustrating genes showing coordinate enrichment or depletion.

    Techniques Used: Selection

    beta lactamase activity  (ATCC)


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    ATCC beta lactamase activity
    Antibiotic screen reveals S. marcescens genes important for growth and survival. A) Screen schematic: An input library (containing about 2 million insertion mutants) is grown to OD 0.1 and divided between 4 conditions for an additional 6 hours of growth: LB alone; LB + cefoxitin 4 ug/mL; LB + cefepime 0.025 ug/mL; and LB + ciprofloxacin 0.05 ug/mL. B) Under screen conditions, the library without drug selection undergoes more than 2 log expansion while those with antibiotic decrease in CFU to similar extent (about 0.5 log), with the library in cefoxitin undergoes expansion after 4 hours, likely due to upregulation of AmpC <t>beta-lactamase.</t> Libraries are harvested for analysis at 6 hours. C) Number of genes with insertion-mutants showing ≥ 4-fold change at 6 hours, compared to outgrowth in LB alone. D) Venn diagram illustrating genes showing coordinate enrichment or depletion.
    Beta Lactamase Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A genome-scale antibiotic screen in Serratia marcescens identifies YdgH as a conserved modifier of cephalosporin and detergent susceptibility"

    Article Title: A genome-scale antibiotic screen in Serratia marcescens identifies YdgH as a conserved modifier of cephalosporin and detergent susceptibility

    Journal: bioRxiv

    doi: 10.1101/2021.04.16.440252

    Antibiotic screen reveals S. marcescens genes important for growth and survival. A) Screen schematic: An input library (containing about 2 million insertion mutants) is grown to OD 0.1 and divided between 4 conditions for an additional 6 hours of growth: LB alone; LB + cefoxitin 4 ug/mL; LB + cefepime 0.025 ug/mL; and LB + ciprofloxacin 0.05 ug/mL. B) Under screen conditions, the library without drug selection undergoes more than 2 log expansion while those with antibiotic decrease in CFU to similar extent (about 0.5 log), with the library in cefoxitin undergoes expansion after 4 hours, likely due to upregulation of AmpC beta-lactamase. Libraries are harvested for analysis at 6 hours. C) Number of genes with insertion-mutants showing ≥ 4-fold change at 6 hours, compared to outgrowth in LB alone. D) Venn diagram illustrating genes showing coordinate enrichment or depletion.
    Figure Legend Snippet: Antibiotic screen reveals S. marcescens genes important for growth and survival. A) Screen schematic: An input library (containing about 2 million insertion mutants) is grown to OD 0.1 and divided between 4 conditions for an additional 6 hours of growth: LB alone; LB + cefoxitin 4 ug/mL; LB + cefepime 0.025 ug/mL; and LB + ciprofloxacin 0.05 ug/mL. B) Under screen conditions, the library without drug selection undergoes more than 2 log expansion while those with antibiotic decrease in CFU to similar extent (about 0.5 log), with the library in cefoxitin undergoes expansion after 4 hours, likely due to upregulation of AmpC beta-lactamase. Libraries are harvested for analysis at 6 hours. C) Number of genes with insertion-mutants showing ≥ 4-fold change at 6 hours, compared to outgrowth in LB alone. D) Venn diagram illustrating genes showing coordinate enrichment or depletion.

    Techniques Used: Selection

    methanol 4 0 water 3 0 strains class mec a gene β lactamase activity antibiotic susceptibility ame oxf meg  (ATCC)


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    ATCC methanol 4 0 water 3 0 strains class mec a gene β lactamase activity antibiotic susceptibility ame oxf meg
    Methanol 4 0 Water 3 0 Strains Class Mec A Gene β Lactamase Activity Antibiotic Susceptibility Ame Oxf Meg, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    weak β lactamase activity  (ATCC)


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    ATCC weak β lactamase activity
    Weak β Lactamase Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC ß lactamase enzyme activity
    Antibiotic-resistance mechanisms and virulence factors in S. aureus isolates. a Assessment of efflux pump activity in five antibiotic-resistant and five susceptible isolates. EtBr efflux assay was performed using 3 µg/mL of EtBr and 30 µg/mL of CPZ. The fluorescent intensity (530 nm/590 nm) was monitored for 60 min after reenergizing the bacterial cells to trigger EtBr efflux with glucose (0.4% v/v). b Assessment of <t>ß-lactamase</t> enzyme activity in the isolates. The isolates were subjected to a Nitrocefin assay where the absorbance of the cell-free extract mixed with Nitrocefin was detected at 490 nm for 15 min. c Distribution of hemolysin manifestation by the 43 isolates. d Alpha hemolysin manifestation by Sa30. Each isolate was cultured in TSA plates with 5% sheep blood for 24 h. The hemolysis was detected visually by the translucency around the bacterial colony. e Distribution of biofilm-forming ability by the 43 isolates. The biofilm formation was assessed using a crystal violet assay. All isolates were classified into weak, moderate, and strong biofilm-formers based on their biofilm-forming ability. f Fluorescence microscopic image of extracellular polymeric substances (EPS) and Sa30 cells. A green (505 nm) filter was used to acquire the GFP-labeled Sa30 biofilms using a high-content screening microscope, Cell Discoverer 7. Alphabets (in tabular data) indicate a significant difference ( p < 0.05). ‘N/A’ stands for not applicable. More data on the resistance mechanisms and virulence factors are provided in Tables S and S . The experiment was performed in quadruplicates and repeated thrice to ensure reproducibility
    ß Lactamase Enzyme Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC β lactamase activity
    Antibiotic-resistance mechanisms and virulence factors in S. aureus isolates. a Assessment of efflux pump activity in five antibiotic-resistant and five susceptible isolates. EtBr efflux assay was performed using 3 µg/mL of EtBr and 30 µg/mL of CPZ. The fluorescent intensity (530 nm/590 nm) was monitored for 60 min after reenergizing the bacterial cells to trigger EtBr efflux with glucose (0.4% v/v). b Assessment of <t>ß-lactamase</t> enzyme activity in the isolates. The isolates were subjected to a Nitrocefin assay where the absorbance of the cell-free extract mixed with Nitrocefin was detected at 490 nm for 15 min. c Distribution of hemolysin manifestation by the 43 isolates. d Alpha hemolysin manifestation by Sa30. Each isolate was cultured in TSA plates with 5% sheep blood for 24 h. The hemolysis was detected visually by the translucency around the bacterial colony. e Distribution of biofilm-forming ability by the 43 isolates. The biofilm formation was assessed using a crystal violet assay. All isolates were classified into weak, moderate, and strong biofilm-formers based on their biofilm-forming ability. f Fluorescence microscopic image of extracellular polymeric substances (EPS) and Sa30 cells. A green (505 nm) filter was used to acquire the GFP-labeled Sa30 biofilms using a high-content screening microscope, Cell Discoverer 7. Alphabets (in tabular data) indicate a significant difference ( p < 0.05). ‘N/A’ stands for not applicable. More data on the resistance mechanisms and virulence factors are provided in Tables S and S . The experiment was performed in quadruplicates and repeated thrice to ensure reproducibility
    β Lactamase Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC negative β lactamase activity
    Antibiotic-resistance mechanisms and virulence factors in S. aureus isolates. a Assessment of efflux pump activity in five antibiotic-resistant and five susceptible isolates. EtBr efflux assay was performed using 3 µg/mL of EtBr and 30 µg/mL of CPZ. The fluorescent intensity (530 nm/590 nm) was monitored for 60 min after reenergizing the bacterial cells to trigger EtBr efflux with glucose (0.4% v/v). b Assessment of <t>ß-lactamase</t> enzyme activity in the isolates. The isolates were subjected to a Nitrocefin assay where the absorbance of the cell-free extract mixed with Nitrocefin was detected at 490 nm for 15 min. c Distribution of hemolysin manifestation by the 43 isolates. d Alpha hemolysin manifestation by Sa30. Each isolate was cultured in TSA plates with 5% sheep blood for 24 h. The hemolysis was detected visually by the translucency around the bacterial colony. e Distribution of biofilm-forming ability by the 43 isolates. The biofilm formation was assessed using a crystal violet assay. All isolates were classified into weak, moderate, and strong biofilm-formers based on their biofilm-forming ability. f Fluorescence microscopic image of extracellular polymeric substances (EPS) and Sa30 cells. A green (505 nm) filter was used to acquire the GFP-labeled Sa30 biofilms using a high-content screening microscope, Cell Discoverer 7. Alphabets (in tabular data) indicate a significant difference ( p < 0.05). ‘N/A’ stands for not applicable. More data on the resistance mechanisms and virulence factors are provided in Tables S and S . The experiment was performed in quadruplicates and repeated thrice to ensure reproducibility
    Negative β Lactamase Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC beta lactamase activity
    Antimicrobial susceptibility and minimum inhibitory concentrations of L. plantarum ATCC 202195-A and L. plantarum ATCC 202195-B.
    Beta Lactamase Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC methanol 4 0 water 3 0 strains class mec a gene β lactamase activity antibiotic susceptibility ame oxf meg
    Antimicrobial susceptibility and minimum inhibitory concentrations of L. plantarum ATCC 202195-A and L. plantarum ATCC 202195-B.
    Methanol 4 0 Water 3 0 Strains Class Mec A Gene β Lactamase Activity Antibiotic Susceptibility Ame Oxf Meg, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC weak β lactamase activity
    Antimicrobial susceptibility and minimum inhibitory concentrations of L. plantarum ATCC 202195-A and L. plantarum ATCC 202195-B.
    Weak β Lactamase Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Antibiotic-resistance mechanisms and virulence factors in S. aureus isolates. a Assessment of efflux pump activity in five antibiotic-resistant and five susceptible isolates. EtBr efflux assay was performed using 3 µg/mL of EtBr and 30 µg/mL of CPZ. The fluorescent intensity (530 nm/590 nm) was monitored for 60 min after reenergizing the bacterial cells to trigger EtBr efflux with glucose (0.4% v/v). b Assessment of ß-lactamase enzyme activity in the isolates. The isolates were subjected to a Nitrocefin assay where the absorbance of the cell-free extract mixed with Nitrocefin was detected at 490 nm for 15 min. c Distribution of hemolysin manifestation by the 43 isolates. d Alpha hemolysin manifestation by Sa30. Each isolate was cultured in TSA plates with 5% sheep blood for 24 h. The hemolysis was detected visually by the translucency around the bacterial colony. e Distribution of biofilm-forming ability by the 43 isolates. The biofilm formation was assessed using a crystal violet assay. All isolates were classified into weak, moderate, and strong biofilm-formers based on their biofilm-forming ability. f Fluorescence microscopic image of extracellular polymeric substances (EPS) and Sa30 cells. A green (505 nm) filter was used to acquire the GFP-labeled Sa30 biofilms using a high-content screening microscope, Cell Discoverer 7. Alphabets (in tabular data) indicate a significant difference ( p < 0.05). ‘N/A’ stands for not applicable. More data on the resistance mechanisms and virulence factors are provided in Tables S and S . The experiment was performed in quadruplicates and repeated thrice to ensure reproducibility

    Journal: BMC Microbiology

    Article Title: Genomic and phenotypic profiling of Staphylococcus aureus isolates from bovine mastitis for antibiotic resistance and intestinal infectivity

    doi: 10.1186/s12866-023-02785-1

    Figure Lengend Snippet: Antibiotic-resistance mechanisms and virulence factors in S. aureus isolates. a Assessment of efflux pump activity in five antibiotic-resistant and five susceptible isolates. EtBr efflux assay was performed using 3 µg/mL of EtBr and 30 µg/mL of CPZ. The fluorescent intensity (530 nm/590 nm) was monitored for 60 min after reenergizing the bacterial cells to trigger EtBr efflux with glucose (0.4% v/v). b Assessment of ß-lactamase enzyme activity in the isolates. The isolates were subjected to a Nitrocefin assay where the absorbance of the cell-free extract mixed with Nitrocefin was detected at 490 nm for 15 min. c Distribution of hemolysin manifestation by the 43 isolates. d Alpha hemolysin manifestation by Sa30. Each isolate was cultured in TSA plates with 5% sheep blood for 24 h. The hemolysis was detected visually by the translucency around the bacterial colony. e Distribution of biofilm-forming ability by the 43 isolates. The biofilm formation was assessed using a crystal violet assay. All isolates were classified into weak, moderate, and strong biofilm-formers based on their biofilm-forming ability. f Fluorescence microscopic image of extracellular polymeric substances (EPS) and Sa30 cells. A green (505 nm) filter was used to acquire the GFP-labeled Sa30 biofilms using a high-content screening microscope, Cell Discoverer 7. Alphabets (in tabular data) indicate a significant difference ( p < 0.05). ‘N/A’ stands for not applicable. More data on the resistance mechanisms and virulence factors are provided in Tables S and S . The experiment was performed in quadruplicates and repeated thrice to ensure reproducibility

    Article Snippet: S. aureus ATCC 25923 with no ß-lactamase enzyme activity was used as a reference strain.

    Techniques: Activity Assay, Cell Culture, Crystal Violet Assay, Fluorescence, Labeling, High Content Screening, Microscopy

    Antimicrobial susceptibility and minimum inhibitory concentrations of L. plantarum ATCC 202195-A and L. plantarum ATCC 202195-B.

    Journal: Scientific Reports

    Article Title: Antimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium Lactiplantibacillus plantarum, strain ATCC 202195

    doi: 10.1038/s41598-021-94997-6

    Figure Lengend Snippet: Antimicrobial susceptibility and minimum inhibitory concentrations of L. plantarum ATCC 202195-A and L. plantarum ATCC 202195-B.

    Article Snippet: L. plantarum ATCC 202195-B was also tested for beta-lactamase activity using Nitrocefin (BD BBL, New Jersey, USA).

    Techniques: Diffusion-based Assay, Activity Assay