Structured Review

Promega β iii tubulin
β-Catenin GOF causes upregulation of <t>β-III</t> TUBULIN in TTR + ChPe cells. ( a – c ) At E12.5, both control and Lmx1aCre::β-Catenin GOF ChP display a ribbon-like morphology and do not express hem markers Wnt2b and BLBP ( a ) but express Ttr mRNA and TTR protein ( b ). ( c ) Co-immunostaining for β-III TUBULIN and TTR identifies cells that display both markers in the β-Catenin GOF but not in the control ChPe. ( a – c ) Representative images are shown of sections taken from N = 3 brains (biologically independent replicates) examined over 2 independent experiments. Boxed regions ( c ) are shown at high magnification in the adjacent panels. ( d – g ) At E13.5, male Lmx1aCre::Ai9 control and Lmx1aCre::Ai9::β-Catenin GOF brains display Cre-driven Ai9-positive cells throughout the ChPe ( d , f ), In contrast, female control and β-Catenin GOF brains show mosaic Ai9 due to random X-inactivation ( e , g ). β-III TUBULIN is undetectable in the female control ChPe, but appears selectively in the Ai9+ patches in the female β-Catenin GOF ChPe ( e , g ). ( h – k ). In females, neither control Lmx1aCre::Ai9 ChPe cells (cells #1–4; h , i ) nor non-Ai9 cells internal control cells in Lmx1aCre::Ai9::β-Catenin GOF ChPe (cells #5–8; j , k ) display co-localization of β-III TUBULIN and TTR. These markers are both detected in Ai9-positive Lmx1aCre::Ai9::β-Catenin GOF ChPe cells (#9–12; l, m ). ( d – l ), Representative images are shown of sections taken from N = 3 brains (biologically independent replicates) examined over 2 independent experiments. ( i , k , m ) Fluorescence intensity quantifications of cells #1–12 from ( h , j , l ). Boxed regions ( e , g ) are shown at high magnification in the panels ( h , j , l ) below. Scale bars: 10 μm (all high mag panels in c and all panels in h – l ); 50 μm (all panels in a – e ). Further information on replicates and reproducibility for this figure is mentioned in the “Statistics and Reproducibility” section of the Methods. Source data are provided as a Source Data file.
β Iii Tubulin, supplied by Promega, used in various techniques. Bioz Stars score: 97/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β iii tubulin - by Bioz Stars, 2022-11
97/100 stars

Images

1) Product Images from "Constitutive activation of canonical Wnt signaling disrupts choroid plexus epithelial fate"

Article Title: Constitutive activation of canonical Wnt signaling disrupts choroid plexus epithelial fate

Journal: Nature Communications

doi: 10.1038/s41467-021-27602-z

β-Catenin GOF causes upregulation of β-III TUBULIN in TTR + ChPe cells. ( a – c ) At E12.5, both control and Lmx1aCre::β-Catenin GOF ChP display a ribbon-like morphology and do not express hem markers Wnt2b and BLBP ( a ) but express Ttr mRNA and TTR protein ( b ). ( c ) Co-immunostaining for β-III TUBULIN and TTR identifies cells that display both markers in the β-Catenin GOF but not in the control ChPe. ( a – c ) Representative images are shown of sections taken from N = 3 brains (biologically independent replicates) examined over 2 independent experiments. Boxed regions ( c ) are shown at high magnification in the adjacent panels. ( d – g ) At E13.5, male Lmx1aCre::Ai9 control and Lmx1aCre::Ai9::β-Catenin GOF brains display Cre-driven Ai9-positive cells throughout the ChPe ( d , f ), In contrast, female control and β-Catenin GOF brains show mosaic Ai9 due to random X-inactivation ( e , g ). β-III TUBULIN is undetectable in the female control ChPe, but appears selectively in the Ai9+ patches in the female β-Catenin GOF ChPe ( e , g ). ( h – k ). In females, neither control Lmx1aCre::Ai9 ChPe cells (cells #1–4; h , i ) nor non-Ai9 cells internal control cells in Lmx1aCre::Ai9::β-Catenin GOF ChPe (cells #5–8; j , k ) display co-localization of β-III TUBULIN and TTR. These markers are both detected in Ai9-positive Lmx1aCre::Ai9::β-Catenin GOF ChPe cells (#9–12; l, m ). ( d – l ), Representative images are shown of sections taken from N = 3 brains (biologically independent replicates) examined over 2 independent experiments. ( i , k , m ) Fluorescence intensity quantifications of cells #1–12 from ( h , j , l ). Boxed regions ( e , g ) are shown at high magnification in the panels ( h , j , l ) below. Scale bars: 10 μm (all high mag panels in c and all panels in h – l ); 50 μm (all panels in a – e ). Further information on replicates and reproducibility for this figure is mentioned in the “Statistics and Reproducibility” section of the Methods. Source data are provided as a Source Data file.
Figure Legend Snippet: β-Catenin GOF causes upregulation of β-III TUBULIN in TTR + ChPe cells. ( a – c ) At E12.5, both control and Lmx1aCre::β-Catenin GOF ChP display a ribbon-like morphology and do not express hem markers Wnt2b and BLBP ( a ) but express Ttr mRNA and TTR protein ( b ). ( c ) Co-immunostaining for β-III TUBULIN and TTR identifies cells that display both markers in the β-Catenin GOF but not in the control ChPe. ( a – c ) Representative images are shown of sections taken from N = 3 brains (biologically independent replicates) examined over 2 independent experiments. Boxed regions ( c ) are shown at high magnification in the adjacent panels. ( d – g ) At E13.5, male Lmx1aCre::Ai9 control and Lmx1aCre::Ai9::β-Catenin GOF brains display Cre-driven Ai9-positive cells throughout the ChPe ( d , f ), In contrast, female control and β-Catenin GOF brains show mosaic Ai9 due to random X-inactivation ( e , g ). β-III TUBULIN is undetectable in the female control ChPe, but appears selectively in the Ai9+ patches in the female β-Catenin GOF ChPe ( e , g ). ( h – k ). In females, neither control Lmx1aCre::Ai9 ChPe cells (cells #1–4; h , i ) nor non-Ai9 cells internal control cells in Lmx1aCre::Ai9::β-Catenin GOF ChPe (cells #5–8; j , k ) display co-localization of β-III TUBULIN and TTR. These markers are both detected in Ai9-positive Lmx1aCre::Ai9::β-Catenin GOF ChPe cells (#9–12; l, m ). ( d – l ), Representative images are shown of sections taken from N = 3 brains (biologically independent replicates) examined over 2 independent experiments. ( i , k , m ) Fluorescence intensity quantifications of cells #1–12 from ( h , j , l ). Boxed regions ( e , g ) are shown at high magnification in the panels ( h , j , l ) below. Scale bars: 10 μm (all high mag panels in c and all panels in h – l ); 50 μm (all panels in a – e ). Further information on replicates and reproducibility for this figure is mentioned in the “Statistics and Reproducibility” section of the Methods. Source data are provided as a Source Data file.

Techniques Used: Immunostaining, Fluorescence

2) Product Images from "Constitutive activation of canonical Wnt signaling disrupts choroid plexus epithelial fate"

Article Title: Constitutive activation of canonical Wnt signaling disrupts choroid plexus epithelial fate

Journal: bioRxiv

doi: 10.1101/2020.12.08.415588

β-Catenin GOF causes upregulation of β-III TUBULIN in E-CADHERIN-positive ChPe cells. (A) Lmx1aCre::β-Catenin GOF::Ai9 female ChPe display mosaic Ai9+ patches interspersed with Ai9-patches. βIII TUBULIN appears selectively in the Ai9+ patches whereas E-CADHERIN staining is seen in both Ai9+ and Ai9-cells. (B) Ai9-internal control cells, do not display detectable βIII TUBULIN but contain E-CADHERIN. (C) Ai9+ cells display co-localization of β-III TUBULIN and E-CADHERIN. Scale bars: 5 μm (B and C); 20 μm (A).
Figure Legend Snippet: β-Catenin GOF causes upregulation of β-III TUBULIN in E-CADHERIN-positive ChPe cells. (A) Lmx1aCre::β-Catenin GOF::Ai9 female ChPe display mosaic Ai9+ patches interspersed with Ai9-patches. βIII TUBULIN appears selectively in the Ai9+ patches whereas E-CADHERIN staining is seen in both Ai9+ and Ai9-cells. (B) Ai9-internal control cells, do not display detectable βIII TUBULIN but contain E-CADHERIN. (C) Ai9+ cells display co-localization of β-III TUBULIN and E-CADHERIN. Scale bars: 5 μm (B and C); 20 μm (A).

Techniques Used: Staining

β-Catenin GOF causes upregulation of β-III TUBULIN in TTR-positive ChPe cells. (A-C) At E12.5, both control and Lmx1aCre::β-Catenin GOF ChP display a ribbon-like morphology and do not express hem Wnt2b and BLBP(A) but express Ttr mRNA and TTR protein (B). (C) Co-immunostaining for β-III TUBULIN and TTR identifies cells that display both markers selectively in the β-Catenin GOF ChPe. (D-G) At E13.5, male Lmx1aCre::Ai9 control and Lmx1aCre::Ai9::β-Catenin GOF brains display Cre-driven Ai9-positive cells throughout the ChPe (D, F). In contrast, female control and β-Catenin GOF brains show mosaic Ai9 due to random X-inactivation. β-III TUBULIN is undetectable in the female control ChPe, but appears selectively in Ai9+ patches in the female β-Catenin GOF ChPe (E,G). (H-K) β-III TUBULIN is undetectable and therefore does not co-localize with TTR staining in Lmx1aCre::Ai9 female control ChPe cells (cells #1-4; H,I) and in non-Ai9 cells internal control cells in Lmx1aCre::Ai9::β-Catenin GOF female ChPe (cells #5-8; J,K). These markers are both detected in Ai9-positive Lmx1aCre::Ai9::β-Catenin GOF female ChPe cells (#9-12; L, M). (I, K, M) Fluorescence intensity quantifications of cells #1-12 from (H,J,L). Scale bars: 10 μm (C high mag H-L); 50 μm (A-E).
Figure Legend Snippet: β-Catenin GOF causes upregulation of β-III TUBULIN in TTR-positive ChPe cells. (A-C) At E12.5, both control and Lmx1aCre::β-Catenin GOF ChP display a ribbon-like morphology and do not express hem Wnt2b and BLBP(A) but express Ttr mRNA and TTR protein (B). (C) Co-immunostaining for β-III TUBULIN and TTR identifies cells that display both markers selectively in the β-Catenin GOF ChPe. (D-G) At E13.5, male Lmx1aCre::Ai9 control and Lmx1aCre::Ai9::β-Catenin GOF brains display Cre-driven Ai9-positive cells throughout the ChPe (D, F). In contrast, female control and β-Catenin GOF brains show mosaic Ai9 due to random X-inactivation. β-III TUBULIN is undetectable in the female control ChPe, but appears selectively in Ai9+ patches in the female β-Catenin GOF ChPe (E,G). (H-K) β-III TUBULIN is undetectable and therefore does not co-localize with TTR staining in Lmx1aCre::Ai9 female control ChPe cells (cells #1-4; H,I) and in non-Ai9 cells internal control cells in Lmx1aCre::Ai9::β-Catenin GOF female ChPe (cells #5-8; J,K). These markers are both detected in Ai9-positive Lmx1aCre::Ai9::β-Catenin GOF female ChPe cells (#9-12; L, M). (I, K, M) Fluorescence intensity quantifications of cells #1-12 from (H,J,L). Scale bars: 10 μm (C high mag H-L); 50 μm (A-E).

Techniques Used: Immunostaining, Staining, Fluorescence

3) Product Images from "Differential expression of drug resistance genes in CD146 positive dental pulp derived stem cells and CD146 negative fibroblasts, et al. Differential expression of drug resistance genes in CD146 positive dental pulp derived stem cells and CD146 negative fibroblasts"

Article Title: Differential expression of drug resistance genes in CD146 positive dental pulp derived stem cells and CD146 negative fibroblasts, et al. Differential expression of drug resistance genes in CD146 positive dental pulp derived stem cells and CD146 negative fibroblasts

Journal: Clinical and Experimental Dental Research

doi: 10.1002/cre2.297

Differentiation assays. (a) Left: Adipocyte with lipid vacuole resulted from adipogenic differentiation of CD146 positive cell stained with oil red. Right: Expression of PAPR‐γ2 and aP‐2 is shown following adipogenic differentiation using PCR. (b) Left: Mineralization and appropriate morphological changes are shown following osteogenic differentiation stained with alizarin red. Right: With osteogenic differentiation, expression of OPN and Col1α1 is revealed by PCR. (c) Left: With neurogenic differentiation typical dendritic cells which express appeared Right: ß‐tubulin III revealed by immune‐fluorescent staining. (d) Left: With hepatocytic differentiation, polygonal/flattened shape cells appeared at day 21 (differentiation step 2) Right: Hepatogenic differentiation was confirmed by qRT‐PCR as hepatogenic related genes were upregulated postdifferentiation, specially ALB and HNF with approximately 10‐ and 2.5‐fold higher expression after differentiation. The bars represent gene expressions before and after differentiation
Figure Legend Snippet: Differentiation assays. (a) Left: Adipocyte with lipid vacuole resulted from adipogenic differentiation of CD146 positive cell stained with oil red. Right: Expression of PAPR‐γ2 and aP‐2 is shown following adipogenic differentiation using PCR. (b) Left: Mineralization and appropriate morphological changes are shown following osteogenic differentiation stained with alizarin red. Right: With osteogenic differentiation, expression of OPN and Col1α1 is revealed by PCR. (c) Left: With neurogenic differentiation typical dendritic cells which express appeared Right: ß‐tubulin III revealed by immune‐fluorescent staining. (d) Left: With hepatocytic differentiation, polygonal/flattened shape cells appeared at day 21 (differentiation step 2) Right: Hepatogenic differentiation was confirmed by qRT‐PCR as hepatogenic related genes were upregulated postdifferentiation, specially ALB and HNF with approximately 10‐ and 2.5‐fold higher expression after differentiation. The bars represent gene expressions before and after differentiation

Techniques Used: Staining, Expressing, Polymerase Chain Reaction, Quantitative RT-PCR

4) Product Images from "Cell-specific and targeted delivery of RNA moieties"

Article Title: Cell-specific and targeted delivery of RNA moieties

Journal: bioRxiv

doi: 10.1101/2020.01.03.893818

legend. Differentiated Neuro2A cells take up CQ-dsRNA complex. (A) Neuro2A cells can be differentiated and expression β III tubulin, a marker for neurons. In addition, all Neuro2A cells express NR2B subunit and co-localize with β III tubulin (merge). (B) Incubation of CQ in culture medium resulted in uptake of CTB by Neuro2A cells, but did not affect NR2B expression, suggesting that CTB-PEG meleimide by itself does not alter expression of proteins. Scale bar: 50μm.
Figure Legend Snippet: legend. Differentiated Neuro2A cells take up CQ-dsRNA complex. (A) Neuro2A cells can be differentiated and expression β III tubulin, a marker for neurons. In addition, all Neuro2A cells express NR2B subunit and co-localize with β III tubulin (merge). (B) Incubation of CQ in culture medium resulted in uptake of CTB by Neuro2A cells, but did not affect NR2B expression, suggesting that CTB-PEG meleimide by itself does not alter expression of proteins. Scale bar: 50μm.

Techniques Used: Expressing, Marker, Incubation, CtB Assay

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    Promega β iii tubulin
    β Iii Tubulin, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega mouse anti β iii tubulin antibody
    Mouse Anti β Iii Tubulin Antibody, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega β iii tubulin antibody
    β Iii Tubulin Antibody, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β iii tubulin antibody/product/Promega
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    β iii tubulin antibody - by Bioz Stars, 2022-11
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    90
    Promega primary antibodies against mouse neuron specific class iii beta tubulin tuj1
    Pregabalin exposure did not affect the total neurite length ( A ) nor the dominant neurite length ( B ), while the number of branches ( C ) and neurites ( D ) were significantly increased in VM non-dopaminergic neurons <t>(TUJ1+/TH−).</t> Representative images and illustrations for VM neurons immunolabeled with TUJ1 in both groups; control ( E , E’ ) and pregabalin-treated ( F , F’ ) groups showed increases in the numbers of branches and neurites in response to pregabalin exposure. Scale bar: 50 μm. Data are represented as mean ± SEM, n = 4 experiments. * p
    Primary Antibodies Against Mouse Neuron Specific Class Iii Beta Tubulin Tuj1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against mouse neuron specific class iii beta tubulin tuj1/product/Promega
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary antibodies against mouse neuron specific class iii beta tubulin tuj1 - by Bioz Stars, 2022-11
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    Pregabalin exposure did not affect the total neurite length ( A ) nor the dominant neurite length ( B ), while the number of branches ( C ) and neurites ( D ) were significantly increased in VM non-dopaminergic neurons (TUJ1+/TH−). Representative images and illustrations for VM neurons immunolabeled with TUJ1 in both groups; control ( E , E’ ) and pregabalin-treated ( F , F’ ) groups showed increases in the numbers of branches and neurites in response to pregabalin exposure. Scale bar: 50 μm. Data are represented as mean ± SEM, n = 4 experiments. * p

    Journal: Cells

    Article Title: The Effects of Prenatal Exposure to Pregabalin on the Development of Ventral Midbrain Dopaminergic Neurons

    doi: 10.3390/cells11050852

    Figure Lengend Snippet: Pregabalin exposure did not affect the total neurite length ( A ) nor the dominant neurite length ( B ), while the number of branches ( C ) and neurites ( D ) were significantly increased in VM non-dopaminergic neurons (TUJ1+/TH−). Representative images and illustrations for VM neurons immunolabeled with TUJ1 in both groups; control ( E , E’ ) and pregabalin-treated ( F , F’ ) groups showed increases in the numbers of branches and neurites in response to pregabalin exposure. Scale bar: 50 μm. Data are represented as mean ± SEM, n = 4 experiments. * p

    Article Snippet: Primary antibodies against mouse neuron-specific class III beta-tubulin (TUJ1) (Promega (Madison, WI, USA), G7121) and tyrosine hydroxylase (TH) (Abcam, Cambridge, UK), ab112) were used.

    Techniques: Immunolabeling