α enolase kinase rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc α enolase kinase rabbit mab
    a Both <t>α-enolase</t> and stathmin were suppressed after 20( S )-Rh2E2 and 20( R )-Rh2E2 treatment. Representative immunoblots and the protein quantification were shown from three independent experiments; **P < 0.01, ***P < 0.001, one-way ANOVA analysis. b Quantification of metastatic markers α-enolase and stathmin genes expression in 20( S ) / ( R )-Rh2E2-treated CCD19Lu cells by RT-qPCR. c 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the expression of α-enolase and stathmin in tumor tissues harvested from LLC-1 xenograft mice. α-enolase and stathmin staining images were representative of 5 tumor sections from 6 animals of each group. The level of signal intensity was scored from 1–5 (5 is maximum) and took the average from five different views of each section taken in ×20 magnifications. d 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the release of lactate in LLC-1 and H1299 cancer cells. *P < 0.05, **P < 0.01, * **P < 0.001, one-way ANOVA analysis.
    α Enolase Kinase Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α enolase kinase rabbit mab/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α enolase kinase rabbit mab - by Bioz Stars, 2023-09
    95/100 stars

    Images

    1) Product Images from "Novel ginsenoside derivative 20( S )-Rh2E2 suppresses tumor growth and metastasis in vivo and in vitro via intervention of cancer cell energy metabolism"

    Article Title: Novel ginsenoside derivative 20( S )-Rh2E2 suppresses tumor growth and metastasis in vivo and in vitro via intervention of cancer cell energy metabolism

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-020-02881-4

    a Both α-enolase and stathmin were suppressed after 20( S )-Rh2E2 and 20( R )-Rh2E2 treatment. Representative immunoblots and the protein quantification were shown from three independent experiments; **P < 0.01, ***P < 0.001, one-way ANOVA analysis. b Quantification of metastatic markers α-enolase and stathmin genes expression in 20( S ) / ( R )-Rh2E2-treated CCD19Lu cells by RT-qPCR. c 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the expression of α-enolase and stathmin in tumor tissues harvested from LLC-1 xenograft mice. α-enolase and stathmin staining images were representative of 5 tumor sections from 6 animals of each group. The level of signal intensity was scored from 1–5 (5 is maximum) and took the average from five different views of each section taken in ×20 magnifications. d 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the release of lactate in LLC-1 and H1299 cancer cells. *P < 0.05, **P < 0.01, * **P < 0.001, one-way ANOVA analysis.
    Figure Legend Snippet: a Both α-enolase and stathmin were suppressed after 20( S )-Rh2E2 and 20( R )-Rh2E2 treatment. Representative immunoblots and the protein quantification were shown from three independent experiments; **P < 0.01, ***P < 0.001, one-way ANOVA analysis. b Quantification of metastatic markers α-enolase and stathmin genes expression in 20( S ) / ( R )-Rh2E2-treated CCD19Lu cells by RT-qPCR. c 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the expression of α-enolase and stathmin in tumor tissues harvested from LLC-1 xenograft mice. α-enolase and stathmin staining images were representative of 5 tumor sections from 6 animals of each group. The level of signal intensity was scored from 1–5 (5 is maximum) and took the average from five different views of each section taken in ×20 magnifications. d 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the release of lactate in LLC-1 and H1299 cancer cells. *P < 0.05, **P < 0.01, * **P < 0.001, one-way ANOVA analysis.

    Techniques Used: Western Blot, Expressing, Quantitative RT-PCR, Staining

    α enolase kinase rabbit mab  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc α enolase kinase rabbit mab
    a Both <t>α-enolase</t> and stathmin were suppressed after 20( S )-Rh2E2 and 20( R )-Rh2E2 treatment. Representative immunoblots and the protein quantification were shown from three independent experiments; **P < 0.01, ***P < 0.001, one-way ANOVA analysis. b Quantification of metastatic markers α-enolase and stathmin genes expression in 20( S ) / ( R )-Rh2E2-treated CCD19Lu cells by RT-qPCR. c 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the expression of α-enolase and stathmin in tumor tissues harvested from LLC-1 xenograft mice. α-enolase and stathmin staining images were representative of 5 tumor sections from 6 animals of each group. The level of signal intensity was scored from 1–5 (5 is maximum) and took the average from five different views of each section taken in ×20 magnifications. d 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the release of lactate in LLC-1 and H1299 cancer cells. *P < 0.05, **P < 0.01, * **P < 0.001, one-way ANOVA analysis.
    α Enolase Kinase Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α enolase kinase rabbit mab/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α enolase kinase rabbit mab - by Bioz Stars, 2023-09
    95/100 stars

    Images

    1) Product Images from "Novel ginsenoside derivative 20( S )-Rh2E2 suppresses tumor growth and metastasis in vivo and in vitro via intervention of cancer cell energy metabolism"

    Article Title: Novel ginsenoside derivative 20( S )-Rh2E2 suppresses tumor growth and metastasis in vivo and in vitro via intervention of cancer cell energy metabolism

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-020-02881-4

    a Both α-enolase and stathmin were suppressed after 20( S )-Rh2E2 and 20( R )-Rh2E2 treatment. Representative immunoblots and the protein quantification were shown from three independent experiments; **P < 0.01, ***P < 0.001, one-way ANOVA analysis. b Quantification of metastatic markers α-enolase and stathmin genes expression in 20( S ) / ( R )-Rh2E2-treated CCD19Lu cells by RT-qPCR. c 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the expression of α-enolase and stathmin in tumor tissues harvested from LLC-1 xenograft mice. α-enolase and stathmin staining images were representative of 5 tumor sections from 6 animals of each group. The level of signal intensity was scored from 1–5 (5 is maximum) and took the average from five different views of each section taken in ×20 magnifications. d 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the release of lactate in LLC-1 and H1299 cancer cells. *P < 0.05, **P < 0.01, * **P < 0.001, one-way ANOVA analysis.
    Figure Legend Snippet: a Both α-enolase and stathmin were suppressed after 20( S )-Rh2E2 and 20( R )-Rh2E2 treatment. Representative immunoblots and the protein quantification were shown from three independent experiments; **P < 0.01, ***P < 0.001, one-way ANOVA analysis. b Quantification of metastatic markers α-enolase and stathmin genes expression in 20( S ) / ( R )-Rh2E2-treated CCD19Lu cells by RT-qPCR. c 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the expression of α-enolase and stathmin in tumor tissues harvested from LLC-1 xenograft mice. α-enolase and stathmin staining images were representative of 5 tumor sections from 6 animals of each group. The level of signal intensity was scored from 1–5 (5 is maximum) and took the average from five different views of each section taken in ×20 magnifications. d 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the release of lactate in LLC-1 and H1299 cancer cells. *P < 0.05, **P < 0.01, * **P < 0.001, one-way ANOVA analysis.

    Techniques Used: Western Blot, Expressing, Quantitative RT-PCR, Staining

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    Cell Signaling Technology Inc α enolase kinase rabbit mab
    a Both <t>α-enolase</t> and stathmin were suppressed after 20( S )-Rh2E2 and 20( R )-Rh2E2 treatment. Representative immunoblots and the protein quantification were shown from three independent experiments; **P < 0.01, ***P < 0.001, one-way ANOVA analysis. b Quantification of metastatic markers α-enolase and stathmin genes expression in 20( S ) / ( R )-Rh2E2-treated CCD19Lu cells by RT-qPCR. c 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the expression of α-enolase and stathmin in tumor tissues harvested from LLC-1 xenograft mice. α-enolase and stathmin staining images were representative of 5 tumor sections from 6 animals of each group. The level of signal intensity was scored from 1–5 (5 is maximum) and took the average from five different views of each section taken in ×20 magnifications. d 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the release of lactate in LLC-1 and H1299 cancer cells. *P < 0.05, **P < 0.01, * **P < 0.001, one-way ANOVA analysis.
    α Enolase Kinase Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α enolase kinase rabbit mab/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α enolase kinase rabbit mab - by Bioz Stars, 2023-09
    95/100 stars
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    a Both α-enolase and stathmin were suppressed after 20( S )-Rh2E2 and 20( R )-Rh2E2 treatment. Representative immunoblots and the protein quantification were shown from three independent experiments; **P < 0.01, ***P < 0.001, one-way ANOVA analysis. b Quantification of metastatic markers α-enolase and stathmin genes expression in 20( S ) / ( R )-Rh2E2-treated CCD19Lu cells by RT-qPCR. c 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the expression of α-enolase and stathmin in tumor tissues harvested from LLC-1 xenograft mice. α-enolase and stathmin staining images were representative of 5 tumor sections from 6 animals of each group. The level of signal intensity was scored from 1–5 (5 is maximum) and took the average from five different views of each section taken in ×20 magnifications. d 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the release of lactate in LLC-1 and H1299 cancer cells. *P < 0.05, **P < 0.01, * **P < 0.001, one-way ANOVA analysis.

    Journal: Cell Death & Disease

    Article Title: Novel ginsenoside derivative 20( S )-Rh2E2 suppresses tumor growth and metastasis in vivo and in vitro via intervention of cancer cell energy metabolism

    doi: 10.1038/s41419-020-02881-4

    Figure Lengend Snippet: a Both α-enolase and stathmin were suppressed after 20( S )-Rh2E2 and 20( R )-Rh2E2 treatment. Representative immunoblots and the protein quantification were shown from three independent experiments; **P < 0.01, ***P < 0.001, one-way ANOVA analysis. b Quantification of metastatic markers α-enolase and stathmin genes expression in 20( S ) / ( R )-Rh2E2-treated CCD19Lu cells by RT-qPCR. c 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the expression of α-enolase and stathmin in tumor tissues harvested from LLC-1 xenograft mice. α-enolase and stathmin staining images were representative of 5 tumor sections from 6 animals of each group. The level of signal intensity was scored from 1–5 (5 is maximum) and took the average from five different views of each section taken in ×20 magnifications. d 20( S )-Rh2E2 and 20( R )-Rh2E2 suppressed the release of lactate in LLC-1 and H1299 cancer cells. *P < 0.05, **P < 0.01, * **P < 0.001, one-way ANOVA analysis.

    Article Snippet: The following reagents from other suppliers were used: phospho-p53 kinase (Ser 15 ) rabbit mAb (CST, 9284, USA), p53 kinase rabbit mAb (CST, 9282, USA), phospho-p38 kinase (Thr 180 /Tyr 182 ) rabbit mAb (CST, 4511, USA), p38 kinase rabbit mAb (CST, 8690, USA), phospho-JNK kinase (Thr 183 /Tyr 185 ) rabbit mAb (CST, 4668, USA), JNK kinase rabbit mAb (CST, 9252, USA), phospho-ERK kinase (Thr 202 /Tyr 204 ) rabbit mAb (CST, 4370, USA), ERK kinase rabbit mAb (CST, 4695, USA), α-enolase kinase rabbit mAb (CST, 3810, USA), stathmin kinase rabbit mAb (CST, 3352, USA), phospho-AMP-activated protein kinase (AMPK; Thr 172 ) rabbit mAb (CST, 2531, USA), AMPK rabbit mAb (CST, 2532, USA), anti-β-of actin mouse monoclonal IgG1 (Santa Cruz, sc-47778, USA), rabbit anti-mouse IgG (H +L) secondary antibody TRITC (Invitrogen, PA1-28565, USA), IRDye 800CW goat anti-mouse IgG (H + L) secondary antibody (Li-COR, 926-32210, USA), IRDye 800CW goat anti-rabbit IgG (H + L) secondary antibody (Li-COR, 926–32211, USA), Compound C (Calbiochem, 171260, USA), SB203580 (CST, 5633, USA), SP600125 (CST, 8177, USA), U0126 (CST, 9903, USA), antibody against LC3-II (CST, 2775, USA).

    Techniques: Western Blot, Expressing, Quantitative RT-PCR, Staining