α enac  (Alomone Labs)


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    Structured Review

    Alomone Labs α enac
    Potential mechanism for PDE-exo-miR-432-5p involved in DSR. The level of PDE-exo-miR-432-5p increases in the PDE of H/HA patients, which can bind to the 3'UTR of <t>α-</t> ENaC and decrease its expression in peritoneal mesothelial cells, affecting the sodium ion transport in PD. Red rectangle, toxin; green triangle, ion; blue ellipse, water; bilayer vesicle, exosome. PDE, peritoneal dialysis effluent; DSR, dialytic sodium removal; H/HA, high/high average group of patients; 3'UTR, 3'untranslated region; PD, peritoneal dialysis.
    α Enac, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α enac/product/Alomone Labs
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    α enac - by Bioz Stars, 2022-08
    93/100 stars

    Images

    1) Product Images from "Peritoneal dialysis effluent-derived exosomal miR-432-5p: an assessment tool for peritoneal dialysis efficacy"

    Article Title: Peritoneal dialysis effluent-derived exosomal miR-432-5p: an assessment tool for peritoneal dialysis efficacy

    Journal: Annals of Translational Medicine

    doi: 10.21037/atm-21-3957

    Potential mechanism for PDE-exo-miR-432-5p involved in DSR. The level of PDE-exo-miR-432-5p increases in the PDE of H/HA patients, which can bind to the 3'UTR of α- ENaC and decrease its expression in peritoneal mesothelial cells, affecting the sodium ion transport in PD. Red rectangle, toxin; green triangle, ion; blue ellipse, water; bilayer vesicle, exosome. PDE, peritoneal dialysis effluent; DSR, dialytic sodium removal; H/HA, high/high average group of patients; 3'UTR, 3'untranslated region; PD, peritoneal dialysis.
    Figure Legend Snippet: Potential mechanism for PDE-exo-miR-432-5p involved in DSR. The level of PDE-exo-miR-432-5p increases in the PDE of H/HA patients, which can bind to the 3'UTR of α- ENaC and decrease its expression in peritoneal mesothelial cells, affecting the sodium ion transport in PD. Red rectangle, toxin; green triangle, ion; blue ellipse, water; bilayer vesicle, exosome. PDE, peritoneal dialysis effluent; DSR, dialytic sodium removal; H/HA, high/high average group of patients; 3'UTR, 3'untranslated region; PD, peritoneal dialysis.

    Techniques Used: Expressing

    Expression of α- ENaC in peritoneum and mesothelial cells. (A) Expression of α- ENaC in human peritoneum determined by IHC. Staining method: IHC. Scale bar: 20 µm. (B) α- ENaC gene expression in mesothelial cells detected by AGE. (C) Detection of α- ENaC protein expression in mesothelial cells by western blot. PBS, phosphate-buffered saline; IHC, immunohistochemistry; AGE, agarose gel electrophoresis.
    Figure Legend Snippet: Expression of α- ENaC in peritoneum and mesothelial cells. (A) Expression of α- ENaC in human peritoneum determined by IHC. Staining method: IHC. Scale bar: 20 µm. (B) α- ENaC gene expression in mesothelial cells detected by AGE. (C) Detection of α- ENaC protein expression in mesothelial cells by western blot. PBS, phosphate-buffered saline; IHC, immunohistochemistry; AGE, agarose gel electrophoresis.

    Techniques Used: Expressing, Immunohistochemistry, Staining, Western Blot, Agarose Gel Electrophoresis

    Verification of SCNN1A as a target gene of miR-432-5p. (A) Predicted alignment of miR-432-5p with the 3'UTR of SCNN1A mRNA. (B,D) The relative expression of miR-432-5p and α- ENaC mRNA measured by qRT-PCR. (C,E) Western blot analysis of α- ENaC protein expression level. (F) Cell viability of Met-5A after transfection detected by CCK-8. (G) Determination of the interaction between miR-432-5p and SCNN1A by the dual luciferase reporter assay. **, P
    Figure Legend Snippet: Verification of SCNN1A as a target gene of miR-432-5p. (A) Predicted alignment of miR-432-5p with the 3'UTR of SCNN1A mRNA. (B,D) The relative expression of miR-432-5p and α- ENaC mRNA measured by qRT-PCR. (C,E) Western blot analysis of α- ENaC protein expression level. (F) Cell viability of Met-5A after transfection detected by CCK-8. (G) Determination of the interaction between miR-432-5p and SCNN1A by the dual luciferase reporter assay. **, P

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Transfection, CCK-8 Assay, Luciferase, Reporter Assay

    2) Product Images from "(Pro)renin receptor contributes to regulation of renal epithelial sodium channel"

    Article Title: (Pro)renin receptor contributes to regulation of renal epithelial sodium channel

    Journal: Journal of hypertension

    doi: 10.1097/HJH.0000000000000825

    Expression of (Pro)renin receptor (PRR) in the kidney. PRR mRNA (a) and protein expression (b) in response to different doses of PRR shRNA. PRR mRNA and protein expression in total kidney tissue (c and d), renal medulla (e and f), and renal medulla α-ENaC
    Figure Legend Snippet: Expression of (Pro)renin receptor (PRR) in the kidney. PRR mRNA (a) and protein expression (b) in response to different doses of PRR shRNA. PRR mRNA and protein expression in total kidney tissue (c and d), renal medulla (e and f), and renal medulla α-ENaC

    Techniques Used: Expressing, shRNA

    3) Product Images from "(Pro)Renin receptor mediates obesity-induced antinatriuresis and elevated blood pressure via upregulation of the renal epithelial sodium channel"

    Article Title: (Pro)Renin receptor mediates obesity-induced antinatriuresis and elevated blood pressure via upregulation of the renal epithelial sodium channel

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0202419

    Renal medullary expression of PRR, SGK-1, and α-ENaC. Renal medullary mRNA (A) and protein (B) expressions of (Pro)renin receptor (PRR), phosphorylation of SGK-1 (C) and total expression of SGK-1 (D), mRNA (E) and protein (F) expressions of α-ENaC in mice fed with regular diet (RD) and high fed diet (HFD) with or without PRR KO. mRNA normalized to β-actin, representative blots (top), quantitative results normalized to β-actin. Data presented as mean ± SEM, NS no significant difference, *p
    Figure Legend Snippet: Renal medullary expression of PRR, SGK-1, and α-ENaC. Renal medullary mRNA (A) and protein (B) expressions of (Pro)renin receptor (PRR), phosphorylation of SGK-1 (C) and total expression of SGK-1 (D), mRNA (E) and protein (F) expressions of α-ENaC in mice fed with regular diet (RD) and high fed diet (HFD) with or without PRR KO. mRNA normalized to β-actin, representative blots (top), quantitative results normalized to β-actin. Data presented as mean ± SEM, NS no significant difference, *p

    Techniques Used: Expressing, Mouse Assay

    4) Product Images from "(Pro)renin receptor contributes to regulation of renal epithelial sodium channel"

    Article Title: (Pro)renin receptor contributes to regulation of renal epithelial sodium channel

    Journal: Journal of hypertension

    doi: 10.1097/HJH.0000000000000825

    Expression of (Pro)renin receptor (PRR) in the kidney. PRR mRNA (a) and protein expression (b) in response to different doses of PRR shRNA. PRR mRNA and protein expression in total kidney tissue (c and d), renal medulla (e and f), and renal medulla α-ENaC
    Figure Legend Snippet: Expression of (Pro)renin receptor (PRR) in the kidney. PRR mRNA (a) and protein expression (b) in response to different doses of PRR shRNA. PRR mRNA and protein expression in total kidney tissue (c and d), renal medulla (e and f), and renal medulla α-ENaC

    Techniques Used: Expressing, shRNA

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    Alomone Labs α enac
    Potential mechanism for PDE-exo-miR-432-5p involved in DSR. The level of PDE-exo-miR-432-5p increases in the PDE of H/HA patients, which can bind to the 3'UTR of <t>α-</t> ENaC and decrease its expression in peritoneal mesothelial cells, affecting the sodium ion transport in PD. Red rectangle, toxin; green triangle, ion; blue ellipse, water; bilayer vesicle, exosome. PDE, peritoneal dialysis effluent; DSR, dialytic sodium removal; H/HA, high/high average group of patients; 3'UTR, 3'untranslated region; PD, peritoneal dialysis.
    α Enac, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α enac/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α enac - by Bioz Stars, 2022-08
    93/100 stars
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    Potential mechanism for PDE-exo-miR-432-5p involved in DSR. The level of PDE-exo-miR-432-5p increases in the PDE of H/HA patients, which can bind to the 3'UTR of α- ENaC and decrease its expression in peritoneal mesothelial cells, affecting the sodium ion transport in PD. Red rectangle, toxin; green triangle, ion; blue ellipse, water; bilayer vesicle, exosome. PDE, peritoneal dialysis effluent; DSR, dialytic sodium removal; H/HA, high/high average group of patients; 3'UTR, 3'untranslated region; PD, peritoneal dialysis.

    Journal: Annals of Translational Medicine

    Article Title: Peritoneal dialysis effluent-derived exosomal miR-432-5p: an assessment tool for peritoneal dialysis efficacy

    doi: 10.21037/atm-21-3957

    Figure Lengend Snippet: Potential mechanism for PDE-exo-miR-432-5p involved in DSR. The level of PDE-exo-miR-432-5p increases in the PDE of H/HA patients, which can bind to the 3'UTR of α- ENaC and decrease its expression in peritoneal mesothelial cells, affecting the sodium ion transport in PD. Red rectangle, toxin; green triangle, ion; blue ellipse, water; bilayer vesicle, exosome. PDE, peritoneal dialysis effluent; DSR, dialytic sodium removal; H/HA, high/high average group of patients; 3'UTR, 3'untranslated region; PD, peritoneal dialysis.

    Article Snippet: Blots were blocked with 5% nonfat milk and then incubated overnight at 4 ℃ with the primary antibodies CD9, CD63, CD81, Hsp70 (1:1,000; SBI Medical, Fort Lauderdale, FL, USA), α- ENaC (1:500; Alomone Labs, Jerusalem, Israel), or GAPDH (1:5,000; Proteintech Group Inc., Rosemont, IL, USA).

    Techniques: Expressing

    Expression of α- ENaC in peritoneum and mesothelial cells. (A) Expression of α- ENaC in human peritoneum determined by IHC. Staining method: IHC. Scale bar: 20 µm. (B) α- ENaC gene expression in mesothelial cells detected by AGE. (C) Detection of α- ENaC protein expression in mesothelial cells by western blot. PBS, phosphate-buffered saline; IHC, immunohistochemistry; AGE, agarose gel electrophoresis.

    Journal: Annals of Translational Medicine

    Article Title: Peritoneal dialysis effluent-derived exosomal miR-432-5p: an assessment tool for peritoneal dialysis efficacy

    doi: 10.21037/atm-21-3957

    Figure Lengend Snippet: Expression of α- ENaC in peritoneum and mesothelial cells. (A) Expression of α- ENaC in human peritoneum determined by IHC. Staining method: IHC. Scale bar: 20 µm. (B) α- ENaC gene expression in mesothelial cells detected by AGE. (C) Detection of α- ENaC protein expression in mesothelial cells by western blot. PBS, phosphate-buffered saline; IHC, immunohistochemistry; AGE, agarose gel electrophoresis.

    Article Snippet: Blots were blocked with 5% nonfat milk and then incubated overnight at 4 ℃ with the primary antibodies CD9, CD63, CD81, Hsp70 (1:1,000; SBI Medical, Fort Lauderdale, FL, USA), α- ENaC (1:500; Alomone Labs, Jerusalem, Israel), or GAPDH (1:5,000; Proteintech Group Inc., Rosemont, IL, USA).

    Techniques: Expressing, Immunohistochemistry, Staining, Western Blot, Agarose Gel Electrophoresis

    Verification of SCNN1A as a target gene of miR-432-5p. (A) Predicted alignment of miR-432-5p with the 3'UTR of SCNN1A mRNA. (B,D) The relative expression of miR-432-5p and α- ENaC mRNA measured by qRT-PCR. (C,E) Western blot analysis of α- ENaC protein expression level. (F) Cell viability of Met-5A after transfection detected by CCK-8. (G) Determination of the interaction between miR-432-5p and SCNN1A by the dual luciferase reporter assay. **, P

    Journal: Annals of Translational Medicine

    Article Title: Peritoneal dialysis effluent-derived exosomal miR-432-5p: an assessment tool for peritoneal dialysis efficacy

    doi: 10.21037/atm-21-3957

    Figure Lengend Snippet: Verification of SCNN1A as a target gene of miR-432-5p. (A) Predicted alignment of miR-432-5p with the 3'UTR of SCNN1A mRNA. (B,D) The relative expression of miR-432-5p and α- ENaC mRNA measured by qRT-PCR. (C,E) Western blot analysis of α- ENaC protein expression level. (F) Cell viability of Met-5A after transfection detected by CCK-8. (G) Determination of the interaction between miR-432-5p and SCNN1A by the dual luciferase reporter assay. **, P

    Article Snippet: Blots were blocked with 5% nonfat milk and then incubated overnight at 4 ℃ with the primary antibodies CD9, CD63, CD81, Hsp70 (1:1,000; SBI Medical, Fort Lauderdale, FL, USA), α- ENaC (1:500; Alomone Labs, Jerusalem, Israel), or GAPDH (1:5,000; Proteintech Group Inc., Rosemont, IL, USA).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Transfection, CCK-8 Assay, Luciferase, Reporter Assay

    Expression of (Pro)renin receptor (PRR) in the kidney. PRR mRNA (a) and protein expression (b) in response to different doses of PRR shRNA. PRR mRNA and protein expression in total kidney tissue (c and d), renal medulla (e and f), and renal medulla α-ENaC

    Journal: Journal of hypertension

    Article Title: (Pro)renin receptor contributes to regulation of renal epithelial sodium channel

    doi: 10.1097/HJH.0000000000000825

    Figure Lengend Snippet: Expression of (Pro)renin receptor (PRR) in the kidney. PRR mRNA (a) and protein expression (b) in response to different doses of PRR shRNA. PRR mRNA and protein expression in total kidney tissue (c and d), renal medulla (e and f), and renal medulla α-ENaC

    Article Snippet: Antibodies to PRR (1 : 1000 dilutions, Abcam, Cambridge, Massachusetts, USA), prorenin/renin (1 : 200 dilutions; Santa Cruz biotechnology, Inc., Santa Cruz, California, USA), SGK-1 (1 : 1000 dilutions, Cell Signaling, USA), p-Nedd4–2 (1 : 1000 dilutions, Abcam, Cambridge, Massachusetts, USA), α-ENaC (1 : 500 dilutions; ASC-030, Alomone Labs, Israel) were used in the Western blot as previously described [ , ].

    Techniques: Expressing, shRNA

    Renal medullary expression of PRR, SGK-1, and α-ENaC. Renal medullary mRNA (A) and protein (B) expressions of (Pro)renin receptor (PRR), phosphorylation of SGK-1 (C) and total expression of SGK-1 (D), mRNA (E) and protein (F) expressions of α-ENaC in mice fed with regular diet (RD) and high fed diet (HFD) with or without PRR KO. mRNA normalized to β-actin, representative blots (top), quantitative results normalized to β-actin. Data presented as mean ± SEM, NS no significant difference, *p

    Journal: PLoS ONE

    Article Title: (Pro)Renin receptor mediates obesity-induced antinatriuresis and elevated blood pressure via upregulation of the renal epithelial sodium channel

    doi: 10.1371/journal.pone.0202419

    Figure Lengend Snippet: Renal medullary expression of PRR, SGK-1, and α-ENaC. Renal medullary mRNA (A) and protein (B) expressions of (Pro)renin receptor (PRR), phosphorylation of SGK-1 (C) and total expression of SGK-1 (D), mRNA (E) and protein (F) expressions of α-ENaC in mice fed with regular diet (RD) and high fed diet (HFD) with or without PRR KO. mRNA normalized to β-actin, representative blots (top), quantitative results normalized to β-actin. Data presented as mean ± SEM, NS no significant difference, *p

    Article Snippet: Western blotAntibodies to PRR (1:1000 dilutions, Abcam, Cambridge, MA, USA), p-SGK-1 (1:1000 dilutions, Cell signaling, USA), SGK-1 (1:1000 dilutions, Cell signaling, USA), α-ENaC (1:500 dilutions; ASC-030, Alamone labs, Israel) were used in the Western blot of renal medullary protein as previously described.

    Techniques: Expressing, Mouse Assay

    ENaC protein and mRNA expression in PCK rats fed diets with varying sodium content. (a) Western blotting showing expression levels of α-ENaC in the renal cortex of the PCK rats fed a SD, NS and HS diets, and a summary graph with densitometry values (normalized to β-actin expression for the same samples). Each lane on the blot is one animal. (b) mRNA expression for α-, β-, and γ-ENaC in the renal cortex of the PCK rats fed a SD, NS and HS diets. (c) Western blotting showing expression levels of AQP2 in the renal cortex of the PCK rats fed a SD, NS and HS diets, and a summary graph with densitometry values (normalized to β-actin expression for the same samples). (d) miRNAs found to be differentially expressed ( p

    Journal: EBioMedicine

    Article Title: Salt-deficient diet exacerbates cystogenesis in ARPKD via epithelial sodium channel (ENaC)

    doi: 10.1016/j.ebiom.2019.01.006

    Figure Lengend Snippet: ENaC protein and mRNA expression in PCK rats fed diets with varying sodium content. (a) Western blotting showing expression levels of α-ENaC in the renal cortex of the PCK rats fed a SD, NS and HS diets, and a summary graph with densitometry values (normalized to β-actin expression for the same samples). Each lane on the blot is one animal. (b) mRNA expression for α-, β-, and γ-ENaC in the renal cortex of the PCK rats fed a SD, NS and HS diets. (c) Western blotting showing expression levels of AQP2 in the renal cortex of the PCK rats fed a SD, NS and HS diets, and a summary graph with densitometry values (normalized to β-actin expression for the same samples). (d) miRNAs found to be differentially expressed ( p

    Article Snippet: Antibodies: α-ENaC (ext) – Alomone Cat #: ASC-030; AQP-2 – SCBT Cat # sc-9882; β-Actin – SCBT Cat # sc-1616 HRP, α-, β-, γ-ENaC –Stressmarq Cat#: SPC-403, SPC-404, and SPC-405 respectively.

    Techniques: Expressing, Western Blot