ω conotoxin gvia (Alomone Labs)


Structured Review

ω Conotoxin Gvia, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ω conotoxin gvia/product/Alomone Labs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The Involvement of Voltage-Operated Calcium Channels in Somato-Dendritic Oxytocin Release"
Article Title: The Involvement of Voltage-Operated Calcium Channels in Somato-Dendritic Oxytocin Release
Journal: PLoS ONE
doi: 10.1371/journal.pone.0025366

Figure Legend Snippet: ( A ) Isolated magnocellular neurons were identified as either vasopressin or oxytocin by their presence or absence of the GFP signal. Scale bar = 10 µm. ( B ) The current-voltage relationships show no difference between whole-cell calcium currents (WCCC) of vasopressin and oxytocin neurons (n = 20 each). ( C ) Normalized conductance (G/G max , fitted using Boltzmann equation) showed no significant change with or without TG. ( D ) Examples of two neurons (both oxytocin neurons), one vehicle treated (•) and one 50 min after pre-treating with thapsigargin (○). The steady-state Ca 2+ current is plotted against time following treatment with channel toxins (in order: 1 µM nicardipine; 200 nM ω-agatoxin IV; 500 nM ω-conotoxin GVIA). The current measured after each toxin was subtracted from that evoked before each treatment to determine the current carried by L-, P/Q- and N-type channels. The remaining current was attributed to R-type channels. ( E ) Examples of N-, L- and WCCC elicited by voltage steps in controls and TG treated cells. ( F ) The resulting current density for each current type normalised for the peak maximal current was averaged (n = 5) to give the average current density from neurons treated with vehicle or thapsigargin. ( G ) In a subsequent experiment L- or N-type channel toxins were administered, alternating the order (n = 5 for each condition). Mean±S.E.M are shown and compared by t-test. * P <0.05 vs control.
Techniques Used: Isolation
ω conotoxin gvia (Alomone Labs)


Structured Review
ω Conotoxin Gvia, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ω conotoxin gvia/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
ω conotoxin gvia (Alomone Labs)


Structured Review
ω Conotoxin Gvia, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ω conotoxin gvia/product/Alomone Labs
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
ω conotoxin gvia (Alomone Labs)


Structured Review

ω Conotoxin Gvia, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ω conotoxin gvia/product/Alomone Labs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "The Involvement of Voltage-Operated Calcium Channels in Somato-Dendritic Oxytocin Release"
Article Title: The Involvement of Voltage-Operated Calcium Channels in Somato-Dendritic Oxytocin Release
Journal: PLoS ONE
doi: 10.1371/journal.pone.0025366

Figure Legend Snippet: ( A ) Isolated magnocellular neurons were identified as either vasopressin or oxytocin by their presence or absence of the GFP signal. Scale bar = 10 µm. ( B ) The current-voltage relationships show no difference between whole-cell calcium currents (WCCC) of vasopressin and oxytocin neurons (n = 20 each). ( C ) Normalized conductance (G/G max , fitted using Boltzmann equation) showed no significant change with or without TG. ( D ) Examples of two neurons (both oxytocin neurons), one vehicle treated (•) and one 50 min after pre-treating with thapsigargin (○). The steady-state Ca 2+ current is plotted against time following treatment with channel toxins (in order: 1 µM nicardipine; 200 nM ω-agatoxin IV; 500 nM ω-conotoxin GVIA). The current measured after each toxin was subtracted from that evoked before each treatment to determine the current carried by L-, P/Q- and N-type channels. The remaining current was attributed to R-type channels. ( E ) Examples of N-, L- and WCCC elicited by voltage steps in controls and TG treated cells. ( F ) The resulting current density for each current type normalised for the peak maximal current was averaged (n = 5) to give the average current density from neurons treated with vehicle or thapsigargin. ( G ) In a subsequent experiment L- or N-type channel toxins were administered, alternating the order (n = 5 for each condition). Mean±S.E.M are shown and compared by t-test. * P <0.05 vs control.
Techniques Used: Isolation
conotoxin gvia (Alomone Labs)


Structured Review

Conotoxin Gvia, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/conotoxin gvia/product/Alomone Labs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "New In Vitro Phenotypic Assay for Epilepsy: Fluorescent Measurement of Synchronized Neuronal Calcium Oscillations"
Article Title: New In Vitro Phenotypic Assay for Epilepsy: Fluorescent Measurement of Synchronized Neuronal Calcium Oscillations
Journal: PLoS ONE
doi: 10.1371/journal.pone.0084755

Figure Legend Snippet: Summary of the compounds and antiepileptic drugs evaluated in the calcium oscillations assay.
Techniques Used:
ω conotoxin gvia ω gvia (Alomone Labs)


Structured Review
![SPC-01 generates functional neurons. (a) Preincubation with Cd 2+ (100 μ M ) together with Ni 2+ (50 μ M ), for 5 minutes significantly reduced the [Ca 2+ ] i responses induced by K + in all cells tested by 93% ± 9.2% ( n = 5), indicating the involvement of voltage-activated Ca 2+ channels in depolarization-induced Ca 2+ entry. (b) The specific L-type Ca 2+ channel blocker nicardipine (1 μ M ) reduced the [Ca 2+ ] i responses by 28% ± 7% ( n = 5; P = 0.002). The trace in (c) shows the [Ca 2+ ] i responses observed in a selected SPC-01-derived neuron induced by 50 m M K + , preincubated for 2 minutes with <t>Ω</t> <t>-GVIA</t> (800 n M ) and then stimulated with K + . After a washout of 10 minutes, the same cells were subjected to K + to observe recovery. Similarly, the effect of the P/Q-Ca 2+ channel blocker, Ω -Aga-IVA, 300 n M was tested before and during stimulation with high K + (d) . After washout of the toxin, the [Ca 2+ ] i response was shown to be reversible. The histogram (e) summarizes the results presented in (c) and (d) . The resting [Ca 2+ ] i level in these cells is indicated as basal. The results are expressed as mean ± SEM; n = 4 (Ω-GVIA) and n = 5 (Ω-Aga-IVA). Asterisks indicate the statistical significance ( P > 0.05) versus control K + stimulus.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6922/pmc03706922/pmc03706922__scrt220-5.jpg)
ω Conotoxin Gvia ω Gvia, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ω conotoxin gvia ω gvia/product/Alomone Labs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Conditionally immortalized stem cell lines from human spinal cord retain regional identity and generate functional V2a interneurons and motorneurons"
Article Title: Conditionally immortalized stem cell lines from human spinal cord retain regional identity and generate functional V2a interneurons and motorneurons
Journal: Stem Cell Research & Therapy
doi: 10.1186/scrt220
![... + , preincubated for 2 minutes with Ω -GVIA (800 n M ) and then stimulated with ... SPC-01 generates functional neurons. (a) Preincubation with Cd 2+ (100 μ M ) together with Ni 2+ (50 μ M ), for 5 minutes significantly reduced the [Ca 2+ ] i responses induced by K + in all cells tested by 93% ± 9.2% ( n = 5), indicating the involvement of voltage-activated Ca 2+ channels in depolarization-induced Ca 2+ entry. (b) The specific L-type Ca 2+ channel blocker nicardipine (1 μ M ) reduced the [Ca 2+ ] i responses by 28% ± 7% ( n = 5; P = 0.002). The trace in (c) shows the [Ca 2+ ] i responses observed in a selected SPC-01-derived neuron induced by 50 m M K + , preincubated for 2 minutes with Ω -GVIA (800 n M ) and then stimulated with K + . After a washout of 10 minutes, the same cells were subjected to K + to observe recovery. Similarly, the effect of the P/Q-Ca 2+ channel blocker, Ω -Aga-IVA, 300 n M was tested before and during stimulation with high K + (d) . After washout of the toxin, the [Ca 2+ ] i response was shown to be reversible. The histogram (e) summarizes the results presented in (c) and (d) . The resting [Ca 2+ ] i level in these cells is indicated as basal. The results are expressed as mean ± SEM; n = 4 (Ω-GVIA) and n = 5 (Ω-Aga-IVA). Asterisks indicate the statistical significance ( P > 0.05) versus control K + stimulus.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6922/pmc03706922/pmc03706922__scrt220-5.jpg)
Figure Legend Snippet: SPC-01 generates functional neurons. (a) Preincubation with Cd 2+ (100 μ M ) together with Ni 2+ (50 μ M ), for 5 minutes significantly reduced the [Ca 2+ ] i responses induced by K + in all cells tested by 93% ± 9.2% ( n = 5), indicating the involvement of voltage-activated Ca 2+ channels in depolarization-induced Ca 2+ entry. (b) The specific L-type Ca 2+ channel blocker nicardipine (1 μ M ) reduced the [Ca 2+ ] i responses by 28% ± 7% ( n = 5; P = 0.002). The trace in (c) shows the [Ca 2+ ] i responses observed in a selected SPC-01-derived neuron induced by 50 m M K + , preincubated for 2 minutes with Ω -GVIA (800 n M ) and then stimulated with K + . After a washout of 10 minutes, the same cells were subjected to K + to observe recovery. Similarly, the effect of the P/Q-Ca 2+ channel blocker, Ω -Aga-IVA, 300 n M was tested before and during stimulation with high K + (d) . After washout of the toxin, the [Ca 2+ ] i response was shown to be reversible. The histogram (e) summarizes the results presented in (c) and (d) . The resting [Ca 2+ ] i level in these cells is indicated as basal. The results are expressed as mean ± SEM; n = 4 (Ω-GVIA) and n = 5 (Ω-Aga-IVA). Asterisks indicate the statistical significance ( P > 0.05) versus control K + stimulus.
Techniques Used: Functional Assay, Derivative Assay
ω conotoxin gvia (Alomone Labs)


Structured Review
ω Conotoxin Gvia, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ω conotoxin gvia/product/Alomone Labs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
ω conotoxin gvia (Alomone Labs)


Structured Review
ω Conotoxin Gvia, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ω conotoxin gvia/product/Alomone Labs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
ω conotoxin gvia (Alomone Labs)


Structured Review

ω Conotoxin Gvia, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ω conotoxin gvia/product/Alomone Labs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Characterization of Na + and Ca 2+ Channels in Zebrafish Dorsal Root Ganglion Neurons"
Article Title: Characterization of Na + and Ca 2+ Channels in Zebrafish Dorsal Root Ganglion Neurons
Journal: PLoS ONE
doi: 10.1371/journal.pone.0042602

Figure Legend Snippet: A , Left , Time courses of I Ca amplitude during serial application of nifedipine (10 µM), ω-agatoxin IVA (0.5 µM), ω-conotoxin GVIA (3 µM), SNX-482 (300 nM) and CdCl 2 (100 µM). I Ca was evoked every 10 s by 70 ms test pulses to 0 mV from a holding potential of −80 mV. The horizontal bars indicate the duration of drug application. Right , superimposed current traces obtained at different time points during drug application (labeled as a–f). B , Bar graph representing the mean I Ca inhibition (%) produced by application of the indicated antagonists or toxins. Error bars represent s . e . m . The number of neurons tested is indicated in parentheses. C , Effect of non-dihydropyridine Ca 2+ channel agonist FPL 64176 (FPL) on I Ca in DRG neurons. FPL (1 µM) was applied to rat superior cervical ganglion (SCG) neurons as a positive control (left panel). Note that FPL applied to zebrafish DRG I Ca display neither an increase in macroscopic inward currents nor greatly prolonged trajectory of the tail currents (right panel).
Techniques Used: Labeling, Inhibition, Produced, Positive Control
ω conotoxin gvia (Alomone Labs)


Structured Review

ω Conotoxin Gvia, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ω conotoxin gvia/product/Alomone Labs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "CRMP-2 peptide mediated decrease of high and low voltage-activated calcium channels, attenuation of nociceptor excitability, and anti-nociception in a model of AIDS therapy-induced painful peripheral neuropathy"
Article Title: CRMP-2 peptide mediated decrease of high and low voltage-activated calcium channels, attenuation of nociceptor excitability, and anti-nociception in a model of AIDS therapy-induced painful peripheral neuropathy
Journal: Molecular Pain
doi: 10.1186/1744-8069-8-54

Figure Legend Snippet: Pharmacological and biophysical dissection of TAT-CBD3A6K-mediated block of T-and R-type calcium currents in DRG neurons. ( A ) Representative T- (top) and R-type (bottom) current traces obtained from two separate DRG neurons evoked by 200 ms steps in 5 mV increments from −60 mV to +50 mV, from a holding potential of −90 mV. The extracellular bath solution contained 5 mM Nifedipine (Nif), 200 nM ω-Agatoxin IVA (Aga) and 500 nM ω-Conotoxin GVIA (CTX) to block L-, P/Q-, and N-type calcium currents, respectively. ( B ) Summary of the normalized conductance (G) versus voltage relations for DRG neurons with T- (filled squares) or R- (open squares) type calcium currents. The dotted line at −10 mV highlights the clear discrimination in conductances between T- and R-type currents. ( C ) Representative currents, evoked by a ramp depolarizations from −60 mV to +20 mV for 2 s, illustrating the presence of both T- and R-type currents in the same DRG neuron before (left trace) and 2 min after application 10 μM TAT-CBD3A6K (right trace)
Techniques Used: Dissection, Blocking Assay

Figure Legend Snippet: Characterization of TAT-CBD3A6K-mediated inhibition of T- and R-type calcium currents. ( A ) Representative family of traces from a DRG neuron with both T- and R-type calcium currents before ( left ), 2 min ( middle ) and 5 min ( right ) after addition of 10 μM TAT-CBD3A6K. Currents were elicited in response to the voltage protocol described in the legend to Figure A. To isolate T- and R-type calcium currents, the extracellular bath solution contained 5 mM Nifedipine (Nif), 200 nM ω-Agatoxin IVA (Aga) and 500 nM ω-Conotoxin GVIA (CTX) to block L-, P/Q-, and N-type calcium currents, respectively. ( B , C ) Time course of TAT-CBD3A6K mediated inhibition (“run-down”) of T-type ( B ) and R-type ( C ) calcium currents. Time course of inhibition is shown as averaged normalized current density (pA pF -1 ) before peptide addition and at intervals of 30 s for 5 min. Averaged values are shown with standard error for 4–6 control cells and 4 cells following addition of 10 μM TAT-CBD3A6K. The asterisk denotes statistical significance (p < 0.05; Student’s t -test) compared to the corresponding control time point. Some error bars are smaller than the symbols. Data represent mean ± SEM from n = 3–6 cells at each time point except for n = 2 the 4 min time point for T-type currents in the presence of peptide
Techniques Used: Inhibition, Blocking Assay
ca v 2 2 n type antagonist ω conotoxin gvia (Alomone Labs)


Structured Review
Ca V 2 2 N Type Antagonist ω Conotoxin Gvia, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ca v 2 2 n type antagonist ω conotoxin gvia/product/Alomone Labs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99