α btx  (Alomone Labs)


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    Alomone Labs α btx
    Delayed release in ω-conotoxin GVIA-treated fish is also observed by means of the exocytotic indicator synaptopHluorin. ( A ) Images taken from a single focal plane of the CaP motor neuron terminals showing the motor neuron fill with Alexa Fluor 647 (gray), postsynaptic labeling with <t>α-btx</t> (red), peak stimulus-induced synaptopHluorin fluorescence (green) and α-btx/synaptopHluorin overlay. These images are shown for a single control (left) and ω-conotoxin GVIA-treated (right) fish. The scale bar corresponds to 10 μm. ( B ) Stimulus-driven fluorescence change, expressed as ΔF/F 0 for the three representative boutons shown for control (gray) and ω-conotoxin GVIA treated (colored) synaptopHluorin motor neurons. The synaptopHluorin signal was measured at each ROI during the time course of 100 Hz stimulation (indicated by the black bar in B ). ( C ) The histogram showing time required to reach 50% maximal fluorescence increase for boutons of control (gray, 127 boutons from 8 fish) and ω-conotoxin GVIA-treated fish (red, 55 boutons from 4 fish). Experiments were performed with 5 mM EGTA in the intracellular solution. DOI: http://dx.doi.org/10.7554/eLife.01206.005
    α Btx, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α btx/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α btx - by Bioz Stars, 2022-08
    91/100 stars

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    1) Product Images from "Synchronous and asynchronous modes of synaptic transmission utilize different calcium sources"

    Article Title: Synchronous and asynchronous modes of synaptic transmission utilize different calcium sources

    Journal: eLife

    doi: 10.7554/eLife.01206

    Delayed release in ω-conotoxin GVIA-treated fish is also observed by means of the exocytotic indicator synaptopHluorin. ( A ) Images taken from a single focal plane of the CaP motor neuron terminals showing the motor neuron fill with Alexa Fluor 647 (gray), postsynaptic labeling with α-btx (red), peak stimulus-induced synaptopHluorin fluorescence (green) and α-btx/synaptopHluorin overlay. These images are shown for a single control (left) and ω-conotoxin GVIA-treated (right) fish. The scale bar corresponds to 10 μm. ( B ) Stimulus-driven fluorescence change, expressed as ΔF/F 0 for the three representative boutons shown for control (gray) and ω-conotoxin GVIA treated (colored) synaptopHluorin motor neurons. The synaptopHluorin signal was measured at each ROI during the time course of 100 Hz stimulation (indicated by the black bar in B ). ( C ) The histogram showing time required to reach 50% maximal fluorescence increase for boutons of control (gray, 127 boutons from 8 fish) and ω-conotoxin GVIA-treated fish (red, 55 boutons from 4 fish). Experiments were performed with 5 mM EGTA in the intracellular solution. DOI: http://dx.doi.org/10.7554/eLife.01206.005
    Figure Legend Snippet: Delayed release in ω-conotoxin GVIA-treated fish is also observed by means of the exocytotic indicator synaptopHluorin. ( A ) Images taken from a single focal plane of the CaP motor neuron terminals showing the motor neuron fill with Alexa Fluor 647 (gray), postsynaptic labeling with α-btx (red), peak stimulus-induced synaptopHluorin fluorescence (green) and α-btx/synaptopHluorin overlay. These images are shown for a single control (left) and ω-conotoxin GVIA-treated (right) fish. The scale bar corresponds to 10 μm. ( B ) Stimulus-driven fluorescence change, expressed as ΔF/F 0 for the three representative boutons shown for control (gray) and ω-conotoxin GVIA treated (colored) synaptopHluorin motor neurons. The synaptopHluorin signal was measured at each ROI during the time course of 100 Hz stimulation (indicated by the black bar in B ). ( C ) The histogram showing time required to reach 50% maximal fluorescence increase for boutons of control (gray, 127 boutons from 8 fish) and ω-conotoxin GVIA-treated fish (red, 55 boutons from 4 fish). Experiments were performed with 5 mM EGTA in the intracellular solution. DOI: http://dx.doi.org/10.7554/eLife.01206.005

    Techniques Used: Fluorescence In Situ Hybridization, Labeling, Fluorescence

    Stimulus-driven calcium signals in CaP motor neuron terminals occurred at synaptic boutons. The fill corresponds to a maximal intensity projection image of the motor neuron filled with Alexa Fluor 647. An arrowhead indicates the soma. A single plane of focus in the filled neuron showing postsynaptic α-btx label, peak Fluo-4 calcium signal and merged α-btx and Fluo-4 signal. The scale bar corresponds to 10 μm. DOI: http://dx.doi.org/10.7554/eLife.01206.006
    Figure Legend Snippet: Stimulus-driven calcium signals in CaP motor neuron terminals occurred at synaptic boutons. The fill corresponds to a maximal intensity projection image of the motor neuron filled with Alexa Fluor 647. An arrowhead indicates the soma. A single plane of focus in the filled neuron showing postsynaptic α-btx label, peak Fluo-4 calcium signal and merged α-btx and Fluo-4 signal. The scale bar corresponds to 10 μm. DOI: http://dx.doi.org/10.7554/eLife.01206.006

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    Alomone Labs α btx
    Delayed release in ω-conotoxin GVIA-treated fish is also observed by means of the exocytotic indicator synaptopHluorin. ( A ) Images taken from a single focal plane of the CaP motor neuron terminals showing the motor neuron fill with Alexa Fluor 647 (gray), postsynaptic labeling with <t>α-btx</t> (red), peak stimulus-induced synaptopHluorin fluorescence (green) and α-btx/synaptopHluorin overlay. These images are shown for a single control (left) and ω-conotoxin GVIA-treated (right) fish. The scale bar corresponds to 10 μm. ( B ) Stimulus-driven fluorescence change, expressed as ΔF/F 0 for the three representative boutons shown for control (gray) and ω-conotoxin GVIA treated (colored) synaptopHluorin motor neurons. The synaptopHluorin signal was measured at each ROI during the time course of 100 Hz stimulation (indicated by the black bar in B ). ( C ) The histogram showing time required to reach 50% maximal fluorescence increase for boutons of control (gray, 127 boutons from 8 fish) and ω-conotoxin GVIA-treated fish (red, 55 boutons from 4 fish). Experiments were performed with 5 mM EGTA in the intracellular solution. DOI: http://dx.doi.org/10.7554/eLife.01206.005
    α Btx, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α btx/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α btx - by Bioz Stars, 2022-08
    91/100 stars
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    Delayed release in ω-conotoxin GVIA-treated fish is also observed by means of the exocytotic indicator synaptopHluorin. ( A ) Images taken from a single focal plane of the CaP motor neuron terminals showing the motor neuron fill with Alexa Fluor 647 (gray), postsynaptic labeling with α-btx (red), peak stimulus-induced synaptopHluorin fluorescence (green) and α-btx/synaptopHluorin overlay. These images are shown for a single control (left) and ω-conotoxin GVIA-treated (right) fish. The scale bar corresponds to 10 μm. ( B ) Stimulus-driven fluorescence change, expressed as ΔF/F 0 for the three representative boutons shown for control (gray) and ω-conotoxin GVIA treated (colored) synaptopHluorin motor neurons. The synaptopHluorin signal was measured at each ROI during the time course of 100 Hz stimulation (indicated by the black bar in B ). ( C ) The histogram showing time required to reach 50% maximal fluorescence increase for boutons of control (gray, 127 boutons from 8 fish) and ω-conotoxin GVIA-treated fish (red, 55 boutons from 4 fish). Experiments were performed with 5 mM EGTA in the intracellular solution. DOI: http://dx.doi.org/10.7554/eLife.01206.005

    Journal: eLife

    Article Title: Synchronous and asynchronous modes of synaptic transmission utilize different calcium sources

    doi: 10.7554/eLife.01206

    Figure Lengend Snippet: Delayed release in ω-conotoxin GVIA-treated fish is also observed by means of the exocytotic indicator synaptopHluorin. ( A ) Images taken from a single focal plane of the CaP motor neuron terminals showing the motor neuron fill with Alexa Fluor 647 (gray), postsynaptic labeling with α-btx (red), peak stimulus-induced synaptopHluorin fluorescence (green) and α-btx/synaptopHluorin overlay. These images are shown for a single control (left) and ω-conotoxin GVIA-treated (right) fish. The scale bar corresponds to 10 μm. ( B ) Stimulus-driven fluorescence change, expressed as ΔF/F 0 for the three representative boutons shown for control (gray) and ω-conotoxin GVIA treated (colored) synaptopHluorin motor neurons. The synaptopHluorin signal was measured at each ROI during the time course of 100 Hz stimulation (indicated by the black bar in B ). ( C ) The histogram showing time required to reach 50% maximal fluorescence increase for boutons of control (gray, 127 boutons from 8 fish) and ω-conotoxin GVIA-treated fish (red, 55 boutons from 4 fish). Experiments were performed with 5 mM EGTA in the intracellular solution. DOI: http://dx.doi.org/10.7554/eLife.01206.005

    Article Snippet: TTX, ω-conotoxin GVIA and α-btx were obtained from Alomone Labs (Jerusalem, Israel).

    Techniques: Fluorescence In Situ Hybridization, Labeling, Fluorescence

    Stimulus-driven calcium signals in CaP motor neuron terminals occurred at synaptic boutons. The fill corresponds to a maximal intensity projection image of the motor neuron filled with Alexa Fluor 647. An arrowhead indicates the soma. A single plane of focus in the filled neuron showing postsynaptic α-btx label, peak Fluo-4 calcium signal and merged α-btx and Fluo-4 signal. The scale bar corresponds to 10 μm. DOI: http://dx.doi.org/10.7554/eLife.01206.006

    Journal: eLife

    Article Title: Synchronous and asynchronous modes of synaptic transmission utilize different calcium sources

    doi: 10.7554/eLife.01206

    Figure Lengend Snippet: Stimulus-driven calcium signals in CaP motor neuron terminals occurred at synaptic boutons. The fill corresponds to a maximal intensity projection image of the motor neuron filled with Alexa Fluor 647. An arrowhead indicates the soma. A single plane of focus in the filled neuron showing postsynaptic α-btx label, peak Fluo-4 calcium signal and merged α-btx and Fluo-4 signal. The scale bar corresponds to 10 μm. DOI: http://dx.doi.org/10.7554/eLife.01206.006

    Article Snippet: TTX, ω-conotoxin GVIA and α-btx were obtained from Alomone Labs (Jerusalem, Israel).

    Techniques: