β actin hrp  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β actin hrp
    β Actin Hrp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β actin hrp/product/Cell Signaling Technology Inc
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    β actin  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc β actin
    β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse anti β actin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti β actin
    Mouse Anti β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    α smooth muscle actin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc α smooth muscle actin
    α Smooth Muscle Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    β actin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β actin
    AMPK activation was required for the inhibitory effect of curcumin on LPS-activated astrocytes. (a) Western blot bands for the protein expression of phospho-AMPKα (Thr172) and AMPKα (n = 3). (b) Densitometric analysis was performed to obtain the relative expression level of target proteins with <t>β-actin</t> as the internal reference. (c) Representative images of GFAP localization by immunofluorescence staining. Astrocytes were pretreated with or without Compound C (10 µM) or AICAR (1 mM) for 1 h before incubation with Curcumin or the vehicle for 24 h, and then exposed to LPS. Scale bar = 20 um. (d) Western blot analysis of GFAP expression. (e) The relative expressions of GFAP were quantified by normalizing to β-actin. ** P < 0.001 vs. the control group. ## P < 0.001 vs. the LPS group. § P < 0.05 vs. LPS + curcumin group.
    β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β actin/product/Cell Signaling Technology Inc
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    β actin - by Bioz Stars, 2023-09
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    1) Product Images from "Curcumin protects from LPS-induced activation of astrocytes via AMPK pathway"

    Article Title: Curcumin protects from LPS-induced activation of astrocytes via AMPK pathway

    Journal: Neuroreport

    doi: 10.1097/WNR.0000000000001950

    AMPK activation was required for the inhibitory effect of curcumin on LPS-activated astrocytes. (a) Western blot bands for the protein expression of phospho-AMPKα (Thr172) and AMPKα (n = 3). (b) Densitometric analysis was performed to obtain the relative expression level of target proteins with β-actin as the internal reference. (c) Representative images of GFAP localization by immunofluorescence staining. Astrocytes were pretreated with or without Compound C (10 µM) or AICAR (1 mM) for 1 h before incubation with Curcumin or the vehicle for 24 h, and then exposed to LPS. Scale bar = 20 um. (d) Western blot analysis of GFAP expression. (e) The relative expressions of GFAP were quantified by normalizing to β-actin. ** P < 0.001 vs. the control group. ## P < 0.001 vs. the LPS group. § P < 0.05 vs. LPS + curcumin group.
    Figure Legend Snippet: AMPK activation was required for the inhibitory effect of curcumin on LPS-activated astrocytes. (a) Western blot bands for the protein expression of phospho-AMPKα (Thr172) and AMPKα (n = 3). (b) Densitometric analysis was performed to obtain the relative expression level of target proteins with β-actin as the internal reference. (c) Representative images of GFAP localization by immunofluorescence staining. Astrocytes were pretreated with or without Compound C (10 µM) or AICAR (1 mM) for 1 h before incubation with Curcumin or the vehicle for 24 h, and then exposed to LPS. Scale bar = 20 um. (d) Western blot analysis of GFAP expression. (e) The relative expressions of GFAP were quantified by normalizing to β-actin. ** P < 0.001 vs. the control group. ## P < 0.001 vs. the LPS group. § P < 0.05 vs. LPS + curcumin group.

    Techniques Used: Activation Assay, Western Blot, Expressing, Immunofluorescence, Staining, Incubation

    The pathways of AMPK were involved in curcumin-suppressed migration in LPS-activated astrocytes. (a) Analysis of the effect of curcumin on the migration of LPS-activated astrocytes by Transwell migration assay. (b) The data of migrated cells were counted and indicted. ** P < 0.001 vs. the control group. ## P < 0.001 vs. the LPS group. Data are expressed as the mean ± SD of three independent experiments. (c) Astrocytes were pretreated with or without Compound C (10 µM) or AICAR (1 mM) for 1 h before incubation with Curcumin or the vehicle for 24 h, and then exposed to LPS. (d) Western blot analysis of MMP2, MMP9, and uPA. (d) The relative expressions of MMP2, MMP9, and uPA were quantified by normalizing to β-actin, respectively. All data are represented as mean ± SD, and obtained from three independent experiments. ** P < 0.001 vs. the control group. ## P < 0.001 vs. the LPS group. § P < 0.05 vs. LPS + curcumin group.
    Figure Legend Snippet: The pathways of AMPK were involved in curcumin-suppressed migration in LPS-activated astrocytes. (a) Analysis of the effect of curcumin on the migration of LPS-activated astrocytes by Transwell migration assay. (b) The data of migrated cells were counted and indicted. ** P < 0.001 vs. the control group. ## P < 0.001 vs. the LPS group. Data are expressed as the mean ± SD of three independent experiments. (c) Astrocytes were pretreated with or without Compound C (10 µM) or AICAR (1 mM) for 1 h before incubation with Curcumin or the vehicle for 24 h, and then exposed to LPS. (d) Western blot analysis of MMP2, MMP9, and uPA. (d) The relative expressions of MMP2, MMP9, and uPA were quantified by normalizing to β-actin, respectively. All data are represented as mean ± SD, and obtained from three independent experiments. ** P < 0.001 vs. the control group. ## P < 0.001 vs. the LPS group. § P < 0.05 vs. LPS + curcumin group.

    Techniques Used: Migration, Transwell Migration Assay, Incubation, Western Blot

    Curcumin reversed the LPS-induced autophagy via AMPK pathway. (a) The expression levels of the autophagy-related proteins including LC3-II, Beclin-1, and P62 were investigated by western blots. (b) The quantitative analysis results of LC3-II/ LC3-I were shown. (c) The relative expression of Beclin-1 was quantified by normalizing it to β-actin. (d) The relative expression of P62 was quantified by normalizing it to β-actin. All data are represented as mean ± SD, and obtained from three independent experiments. ** P < 0.001 vs. the control group. ## P < 0.001 vs. the LPS group. § P < 0.05 vs. LPS + curcumin group.
    Figure Legend Snippet: Curcumin reversed the LPS-induced autophagy via AMPK pathway. (a) The expression levels of the autophagy-related proteins including LC3-II, Beclin-1, and P62 were investigated by western blots. (b) The quantitative analysis results of LC3-II/ LC3-I were shown. (c) The relative expression of Beclin-1 was quantified by normalizing it to β-actin. (d) The relative expression of P62 was quantified by normalizing it to β-actin. All data are represented as mean ± SD, and obtained from three independent experiments. ** P < 0.001 vs. the control group. ## P < 0.001 vs. the LPS group. § P < 0.05 vs. LPS + curcumin group.

    Techniques Used: Expressing, Western Blot

    β actin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β actin
    β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β actin/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    b actin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc b actin
    B Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b actin/product/Cell Signaling Technology Inc
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    β actin antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β actin antibody
    β Actin Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β actin antibody/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    β actin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc β actin
    β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β actin/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    rabbit polyclonal antibody against β actin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal antibody against β actin
    Rabbit Polyclonal Antibody Against β Actin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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