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PerkinElmer ・ geneamp pcr system 2400
・ Geneamp Pcr System 2400, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/・ geneamp pcr system 2400/product/PerkinElmer
Average 80 stars, based on 1 article reviews
Price from $9.99 to $1999.99
・ geneamp pcr system 2400 - by Bioz Stars, 2020-01
80/100 stars

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Nucleic Acid Electrophoresis:

Article Title: Comparative genomics-based identification and analysis of cis-regulatory elements
Article Snippet: .. ・ 50 mM MgSO4 (included in a set of the Platinum Pfx DNA polymerase) ・ dNTP mixture, 2.5 mM each (Invitrogen) ・ Bam HI: 10-20 U/μL (New England Biolabs) ・ Xho I: 10-20 U/μL (New England Biolabs) ・ 10× NEB3 buffer (for double-digestion with Bam HI and Xho I, New England Biolabs) ・ 1 × TAE buffer: 40 mM Tris-acetate, 1mM EDTA, pH 8.0 ・ Agarose (molecular biology grade) ・ Ethidium bromide solution: 10 μg/mL (Sigma) ・ TE buffer: 10 mM Tris-HCl, 1mM EDTA, pH 8.0 ・ 10 mM KCl ・ Ethanol (96-100%, molecular biology grade) ・ 70% Ethanol (prepare by mixing 96-100% ethanol and distilled water) ・ Distilled water ・ Heating block or water bath (for restriction enzyme digestion at 37°C and for melting agarose gel at 50°C) ・ GeneAmp PCR System 2400 (PerkinElmer) or an equivalent standard thermal cycler ・ UV transilluminator (for visualizing ethidium bromide-stained DNA after gel electrophoresis) ・ Microcentrifuge ..

Amplification:

Article Title: Comparative genomics-based identification and analysis of cis-regulatory elements
Article Snippet: ・ QIAquick PCR Purification Kit (Qiagen) ・ QIAquick Gel Extraction kit (Qiagen) ・ Primer pairs for amplification of CNEs: 10 pmol/μL in TE (see Methods for details), stored at −20°C. .. ・ 50 mM MgSO4 (included in a set of the Platinum Pfx DNA polymerase) ・ dNTP mixture, 2.5 mM each (Invitrogen) ・ Bam HI: 10-20 U/μL (New England Biolabs) ・ Xho I: 10-20 U/μL (New England Biolabs) ・ 10× NEB3 buffer (for double-digestion with Bam HI and Xho I, New England Biolabs) ・ 1 × TAE buffer: 40 mM Tris-acetate, 1mM EDTA, pH 8.0 ・ Agarose (molecular biology grade) ・ Ethidium bromide solution: 10 μg/mL (Sigma) ・ TE buffer: 10 mM Tris-HCl, 1mM EDTA, pH 8.0 ・ 10 mM KCl ・ Ethanol (96-100%, molecular biology grade) ・ 70% Ethanol (prepare by mixing 96-100% ethanol and distilled water) ・ Distilled water ・ Heating block or water bath (for restriction enzyme digestion at 37°C and for melting agarose gel at 50°C) ・ GeneAmp PCR System 2400 (PerkinElmer) or an equivalent standard thermal cycler ・ UV transilluminator (for visualizing ethidium bromide-stained DNA after gel electrophoresis) ・ Microcentrifuge

Agarose Gel Electrophoresis:

Article Title: Comparative genomics-based identification and analysis of cis-regulatory elements
Article Snippet: .. ・ 50 mM MgSO4 (included in a set of the Platinum Pfx DNA polymerase) ・ dNTP mixture, 2.5 mM each (Invitrogen) ・ Bam HI: 10-20 U/μL (New England Biolabs) ・ Xho I: 10-20 U/μL (New England Biolabs) ・ 10× NEB3 buffer (for double-digestion with Bam HI and Xho I, New England Biolabs) ・ 1 × TAE buffer: 40 mM Tris-acetate, 1mM EDTA, pH 8.0 ・ Agarose (molecular biology grade) ・ Ethidium bromide solution: 10 μg/mL (Sigma) ・ TE buffer: 10 mM Tris-HCl, 1mM EDTA, pH 8.0 ・ 10 mM KCl ・ Ethanol (96-100%, molecular biology grade) ・ 70% Ethanol (prepare by mixing 96-100% ethanol and distilled water) ・ Distilled water ・ Heating block or water bath (for restriction enzyme digestion at 37°C and for melting agarose gel at 50°C) ・ GeneAmp PCR System 2400 (PerkinElmer) or an equivalent standard thermal cycler ・ UV transilluminator (for visualizing ethidium bromide-stained DNA after gel electrophoresis) ・ Microcentrifuge ..

Blocking Assay:

Article Title: Comparative genomics-based identification and analysis of cis-regulatory elements
Article Snippet: .. ・ 50 mM MgSO4 (included in a set of the Platinum Pfx DNA polymerase) ・ dNTP mixture, 2.5 mM each (Invitrogen) ・ Bam HI: 10-20 U/μL (New England Biolabs) ・ Xho I: 10-20 U/μL (New England Biolabs) ・ 10× NEB3 buffer (for double-digestion with Bam HI and Xho I, New England Biolabs) ・ 1 × TAE buffer: 40 mM Tris-acetate, 1mM EDTA, pH 8.0 ・ Agarose (molecular biology grade) ・ Ethidium bromide solution: 10 μg/mL (Sigma) ・ TE buffer: 10 mM Tris-HCl, 1mM EDTA, pH 8.0 ・ 10 mM KCl ・ Ethanol (96-100%, molecular biology grade) ・ 70% Ethanol (prepare by mixing 96-100% ethanol and distilled water) ・ Distilled water ・ Heating block or water bath (for restriction enzyme digestion at 37°C and for melting agarose gel at 50°C) ・ GeneAmp PCR System 2400 (PerkinElmer) or an equivalent standard thermal cycler ・ UV transilluminator (for visualizing ethidium bromide-stained DNA after gel electrophoresis) ・ Microcentrifuge ..

Purification:

Article Title: Comparative genomics-based identification and analysis of cis-regulatory elements
Article Snippet: ・ QIAquick PCR Purification Kit (Qiagen) ・ QIAquick Gel Extraction kit (Qiagen) ・ Primer pairs for amplification of CNEs: 10 pmol/μL in TE (see Methods for details), stored at −20°C. .. ・ 50 mM MgSO4 (included in a set of the Platinum Pfx DNA polymerase) ・ dNTP mixture, 2.5 mM each (Invitrogen) ・ Bam HI: 10-20 U/μL (New England Biolabs) ・ Xho I: 10-20 U/μL (New England Biolabs) ・ 10× NEB3 buffer (for double-digestion with Bam HI and Xho I, New England Biolabs) ・ 1 × TAE buffer: 40 mM Tris-acetate, 1mM EDTA, pH 8.0 ・ Agarose (molecular biology grade) ・ Ethidium bromide solution: 10 μg/mL (Sigma) ・ TE buffer: 10 mM Tris-HCl, 1mM EDTA, pH 8.0 ・ 10 mM KCl ・ Ethanol (96-100%, molecular biology grade) ・ 70% Ethanol (prepare by mixing 96-100% ethanol and distilled water) ・ Distilled water ・ Heating block or water bath (for restriction enzyme digestion at 37°C and for melting agarose gel at 50°C) ・ GeneAmp PCR System 2400 (PerkinElmer) or an equivalent standard thermal cycler ・ UV transilluminator (for visualizing ethidium bromide-stained DNA after gel electrophoresis) ・ Microcentrifuge

Activity Assay:

Article Title: Comparative genomics-based identification and analysis of cis-regulatory elements
Article Snippet: A plasmid carrying the gata2 basal promoter linked to the GFP-polyA , which is available from R. M. Grainger, may provide more sensitivity for the co-transgenesis assay since it appears to impart higher levels of transcriptional activity to enhancers without causing background expression on its own. .. ・ 50 mM MgSO4 (included in a set of the Platinum Pfx DNA polymerase) ・ dNTP mixture, 2.5 mM each (Invitrogen) ・ Bam HI: 10-20 U/μL (New England Biolabs) ・ Xho I: 10-20 U/μL (New England Biolabs) ・ 10× NEB3 buffer (for double-digestion with Bam HI and Xho I, New England Biolabs) ・ 1 × TAE buffer: 40 mM Tris-acetate, 1mM EDTA, pH 8.0 ・ Agarose (molecular biology grade) ・ Ethidium bromide solution: 10 μg/mL (Sigma) ・ TE buffer: 10 mM Tris-HCl, 1mM EDTA, pH 8.0 ・ 10 mM KCl ・ Ethanol (96-100%, molecular biology grade) ・ 70% Ethanol (prepare by mixing 96-100% ethanol and distilled water) ・ Distilled water ・ Heating block or water bath (for restriction enzyme digestion at 37°C and for melting agarose gel at 50°C) ・ GeneAmp PCR System 2400 (PerkinElmer) or an equivalent standard thermal cycler ・ UV transilluminator (for visualizing ethidium bromide-stained DNA after gel electrophoresis) ・ Microcentrifuge

Expressing:

Article Title: Comparative genomics-based identification and analysis of cis-regulatory elements
Article Snippet: A plasmid carrying the gata2 basal promoter linked to the GFP-polyA , which is available from R. M. Grainger, may provide more sensitivity for the co-transgenesis assay since it appears to impart higher levels of transcriptional activity to enhancers without causing background expression on its own. .. ・ 50 mM MgSO4 (included in a set of the Platinum Pfx DNA polymerase) ・ dNTP mixture, 2.5 mM each (Invitrogen) ・ Bam HI: 10-20 U/μL (New England Biolabs) ・ Xho I: 10-20 U/μL (New England Biolabs) ・ 10× NEB3 buffer (for double-digestion with Bam HI and Xho I, New England Biolabs) ・ 1 × TAE buffer: 40 mM Tris-acetate, 1mM EDTA, pH 8.0 ・ Agarose (molecular biology grade) ・ Ethidium bromide solution: 10 μg/mL (Sigma) ・ TE buffer: 10 mM Tris-HCl, 1mM EDTA, pH 8.0 ・ 10 mM KCl ・ Ethanol (96-100%, molecular biology grade) ・ 70% Ethanol (prepare by mixing 96-100% ethanol and distilled water) ・ Distilled water ・ Heating block or water bath (for restriction enzyme digestion at 37°C and for melting agarose gel at 50°C) ・ GeneAmp PCR System 2400 (PerkinElmer) or an equivalent standard thermal cycler ・ UV transilluminator (for visualizing ethidium bromide-stained DNA after gel electrophoresis) ・ Microcentrifuge

Polymerase Chain Reaction:

Article Title: Comparative genomics-based identification and analysis of cis-regulatory elements
Article Snippet: .. ・ 50 mM MgSO4 (included in a set of the Platinum Pfx DNA polymerase) ・ dNTP mixture, 2.5 mM each (Invitrogen) ・ Bam HI: 10-20 U/μL (New England Biolabs) ・ Xho I: 10-20 U/μL (New England Biolabs) ・ 10× NEB3 buffer (for double-digestion with Bam HI and Xho I, New England Biolabs) ・ 1 × TAE buffer: 40 mM Tris-acetate, 1mM EDTA, pH 8.0 ・ Agarose (molecular biology grade) ・ Ethidium bromide solution: 10 μg/mL (Sigma) ・ TE buffer: 10 mM Tris-HCl, 1mM EDTA, pH 8.0 ・ 10 mM KCl ・ Ethanol (96-100%, molecular biology grade) ・ 70% Ethanol (prepare by mixing 96-100% ethanol and distilled water) ・ Distilled water ・ Heating block or water bath (for restriction enzyme digestion at 37°C and for melting agarose gel at 50°C) ・ GeneAmp PCR System 2400 (PerkinElmer) or an equivalent standard thermal cycler ・ UV transilluminator (for visualizing ethidium bromide-stained DNA after gel electrophoresis) ・ Microcentrifuge ..

Gel Extraction:

Article Title: Comparative genomics-based identification and analysis of cis-regulatory elements
Article Snippet: ・ QIAquick PCR Purification Kit (Qiagen) ・ QIAquick Gel Extraction kit (Qiagen) ・ Primer pairs for amplification of CNEs: 10 pmol/μL in TE (see Methods for details), stored at −20°C. .. ・ 50 mM MgSO4 (included in a set of the Platinum Pfx DNA polymerase) ・ dNTP mixture, 2.5 mM each (Invitrogen) ・ Bam HI: 10-20 U/μL (New England Biolabs) ・ Xho I: 10-20 U/μL (New England Biolabs) ・ 10× NEB3 buffer (for double-digestion with Bam HI and Xho I, New England Biolabs) ・ 1 × TAE buffer: 40 mM Tris-acetate, 1mM EDTA, pH 8.0 ・ Agarose (molecular biology grade) ・ Ethidium bromide solution: 10 μg/mL (Sigma) ・ TE buffer: 10 mM Tris-HCl, 1mM EDTA, pH 8.0 ・ 10 mM KCl ・ Ethanol (96-100%, molecular biology grade) ・ 70% Ethanol (prepare by mixing 96-100% ethanol and distilled water) ・ Distilled water ・ Heating block or water bath (for restriction enzyme digestion at 37°C and for melting agarose gel at 50°C) ・ GeneAmp PCR System 2400 (PerkinElmer) or an equivalent standard thermal cycler ・ UV transilluminator (for visualizing ethidium bromide-stained DNA after gel electrophoresis) ・ Microcentrifuge

Plasmid Preparation:

Article Title: Comparative genomics-based identification and analysis of cis-regulatory elements
Article Snippet: A plasmid carrying the gata2 basal promoter linked to the GFP-polyA , which is available from R. M. Grainger, may provide more sensitivity for the co-transgenesis assay since it appears to impart higher levels of transcriptional activity to enhancers without causing background expression on its own. .. ・ 50 mM MgSO4 (included in a set of the Platinum Pfx DNA polymerase) ・ dNTP mixture, 2.5 mM each (Invitrogen) ・ Bam HI: 10-20 U/μL (New England Biolabs) ・ Xho I: 10-20 U/μL (New England Biolabs) ・ 10× NEB3 buffer (for double-digestion with Bam HI and Xho I, New England Biolabs) ・ 1 × TAE buffer: 40 mM Tris-acetate, 1mM EDTA, pH 8.0 ・ Agarose (molecular biology grade) ・ Ethidium bromide solution: 10 μg/mL (Sigma) ・ TE buffer: 10 mM Tris-HCl, 1mM EDTA, pH 8.0 ・ 10 mM KCl ・ Ethanol (96-100%, molecular biology grade) ・ 70% Ethanol (prepare by mixing 96-100% ethanol and distilled water) ・ Distilled water ・ Heating block or water bath (for restriction enzyme digestion at 37°C and for melting agarose gel at 50°C) ・ GeneAmp PCR System 2400 (PerkinElmer) or an equivalent standard thermal cycler ・ UV transilluminator (for visualizing ethidium bromide-stained DNA after gel electrophoresis) ・ Microcentrifuge

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  • 97
    PerkinElmer dna thermal cycler
    Random Amplified Polymorphic <t>DNA</t> <t>(RAPD)</t> profile of Thai and Myanmar strains of Wuchereria bancrofti . The RAPD profile of the Thai and Myanmar strains of W. bancrofti using Primer 1 (5'-dGGTGCGGGAA-3'): Lane M: 100 bp DNA marker; Lane 1–6: the Thai strain of W. bancrofti ; Lane 7–12: the Myanmar strain of W. bancrofti ; Lane C: Negative Control (uninfected human blood sample). The 300 bp and 795 bp fragments (arrows) are specific to the Myanmar strain of W. bancrofti . The 645, 705, 1290, and 1400 bp fragments were common in both the Thai and Myanmar strains of W. bancrofti .
    Dna Thermal Cycler, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 97/100, based on 486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna thermal cycler/product/PerkinElmer
    Average 97 stars, based on 486 article reviews
    Price from $9.99 to $1999.99
    dna thermal cycler - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

    80
    PerkinElmer life sciences geneamp pcr system 2400
    Random Amplified Polymorphic <t>DNA</t> <t>(RAPD)</t> profile of Thai and Myanmar strains of Wuchereria bancrofti . The RAPD profile of the Thai and Myanmar strains of W. bancrofti using Primer 1 (5'-dGGTGCGGGAA-3'): Lane M: 100 bp DNA marker; Lane 1–6: the Thai strain of W. bancrofti ; Lane 7–12: the Myanmar strain of W. bancrofti ; Lane C: Negative Control (uninfected human blood sample). The 300 bp and 795 bp fragments (arrows) are specific to the Myanmar strain of W. bancrofti . The 645, 705, 1290, and 1400 bp fragments were common in both the Thai and Myanmar strains of W. bancrofti .
    Life Sciences Geneamp Pcr System 2400, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/life sciences geneamp pcr system 2400/product/PerkinElmer
    Average 80 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    life sciences geneamp pcr system 2400 - by Bioz Stars, 2020-01
    80/100 stars
      Buy from Supplier

    83
    PerkinElmer 2400 geneamp pcr thermal cycler
    Random Amplified Polymorphic <t>DNA</t> <t>(RAPD)</t> profile of Thai and Myanmar strains of Wuchereria bancrofti . The RAPD profile of the Thai and Myanmar strains of W. bancrofti using Primer 1 (5'-dGGTGCGGGAA-3'): Lane M: 100 bp DNA marker; Lane 1–6: the Thai strain of W. bancrofti ; Lane 7–12: the Myanmar strain of W. bancrofti ; Lane C: Negative Control (uninfected human blood sample). The 300 bp and 795 bp fragments (arrows) are specific to the Myanmar strain of W. bancrofti . The 645, 705, 1290, and 1400 bp fragments were common in both the Thai and Myanmar strains of W. bancrofti .
    2400 Geneamp Pcr Thermal Cycler, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 83/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2400 geneamp pcr thermal cycler/product/PerkinElmer
    Average 83 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2400 geneamp pcr thermal cycler - by Bioz Stars, 2020-01
    83/100 stars
      Buy from Supplier

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    Random Amplified Polymorphic DNA (RAPD) profile of Thai and Myanmar strains of Wuchereria bancrofti . The RAPD profile of the Thai and Myanmar strains of W. bancrofti using Primer 1 (5'-dGGTGCGGGAA-3'): Lane M: 100 bp DNA marker; Lane 1–6: the Thai strain of W. bancrofti ; Lane 7–12: the Myanmar strain of W. bancrofti ; Lane C: Negative Control (uninfected human blood sample). The 300 bp and 795 bp fragments (arrows) are specific to the Myanmar strain of W. bancrofti . The 645, 705, 1290, and 1400 bp fragments were common in both the Thai and Myanmar strains of W. bancrofti .

    Journal: Filaria Journal

    Article Title: Random Amplified Polymorphic DNA (RAPD) for differentiation between Thai and Myanmar strains of Wuchereria bancrofti

    doi: 10.1186/1475-2883-6-6

    Figure Lengend Snippet: Random Amplified Polymorphic DNA (RAPD) profile of Thai and Myanmar strains of Wuchereria bancrofti . The RAPD profile of the Thai and Myanmar strains of W. bancrofti using Primer 1 (5'-dGGTGCGGGAA-3'): Lane M: 100 bp DNA marker; Lane 1–6: the Thai strain of W. bancrofti ; Lane 7–12: the Myanmar strain of W. bancrofti ; Lane C: Negative Control (uninfected human blood sample). The 300 bp and 795 bp fragments (arrows) are specific to the Myanmar strain of W. bancrofti . The 645, 705, 1290, and 1400 bp fragments were common in both the Thai and Myanmar strains of W. bancrofti .

    Article Snippet: The RAPD reaction was performed in a DNA thermal cycler (GeneAmp PCR System 2400, Perkin-Elmer, Norwalk, CT), for 1 cycle at 96°C for 4 min, followed by 40 cycles of 94°C for 1 min, 40°C for 1 min and 72°C for 2 min, respectively.

    Techniques: Amplification, Marker, Negative Control

    Application of DNA polymerase from P. islandicum in PCR. Reactions were carried out in a buffer containing 15 mM Tris-HCl (pH 8.6), 12.5 mM KCl, 2.5 mM (NH 4 ) 2 SO 4 , 1.25 mM MgCl 2 , and 20 μg of BSA per ml. One unit of DNA polymerase from P. islandicum was used to amplify 500 bp (lane 1), 1,000 bp (lane 2), and 1,500 bp (lane 3) of a λ DNA template. Control PCR was performed with 2.5 U of High Fidelity enzyme (lane 4). Twenty microliters of each PCR product was applied on an 1% agarose gel. The corresponding molecular sizes of the marker (lane 5) are indicated in kilobase pairs.

    Journal: Journal of Bacteriology

    Article Title: Cloning and Characterization of a Family B DNA Polymerase from the Hyperthermophilic Crenarchaeon Pyrobaculum islandicum †

    doi:

    Figure Lengend Snippet: Application of DNA polymerase from P. islandicum in PCR. Reactions were carried out in a buffer containing 15 mM Tris-HCl (pH 8.6), 12.5 mM KCl, 2.5 mM (NH 4 ) 2 SO 4 , 1.25 mM MgCl 2 , and 20 μg of BSA per ml. One unit of DNA polymerase from P. islandicum was used to amplify 500 bp (lane 1), 1,000 bp (lane 2), and 1,500 bp (lane 3) of a λ DNA template. Control PCR was performed with 2.5 U of High Fidelity enzyme (lane 4). Twenty microliters of each PCR product was applied on an 1% agarose gel. The corresponding molecular sizes of the marker (lane 5) are indicated in kilobase pairs.

    Article Snippet: After an initial denaturation step at 94°C for 2 min, 30 cycles with a temperature profile of 10 s at 94°C, 30 s at 46°C, and 90 s at 68°C were performed with a DNA thermal cycler (GeneAmp PCR System 2400; Perkin-Elmer).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker