φx174 ssdna  (New England Biolabs)


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    Structured Review

    New England Biolabs φx174 ssdna
    DNA synthesis of DNA pol-prim on natural <t>ssDNA</t> in the presence of RPA and PyV Tag. The cooperation of PyV Tag with mouse, human, and mutant pol-prims was tested on natural ssDNA bound by human RPA. In the reaction, <t>ΦX174</t> ssDNA, RPA, and pol-prim complexes as indicated were incubated in the absence (shaded bars) or in the presence (solid bars) of PyV Tag. The incorporation of acid-insoluble dNMPs in the absence of Tag was arbitrarily set to 1. The presented data are the mean values and standard deviations of three experiments.
    φx174 Ssdna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/φx174 ssdna/product/New England Biolabs
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    φx174 ssdna - by Bioz Stars, 2020-09
    88/100 stars

    Images

    1) Product Images from "Amino Acids 257 to 288 of Mouse p48 Control the Cooperation of Polyomavirus Large T Antigen, Replication Protein A, and DNA Polymerase ?-Primase To Synthesize DNA In Vitro"

    Article Title: Amino Acids 257 to 288 of Mouse p48 Control the Cooperation of Polyomavirus Large T Antigen, Replication Protein A, and DNA Polymerase ?-Primase To Synthesize DNA In Vitro

    Journal: Journal of Virology

    doi: 10.1128/JVI.75.18.8569-8578.2001

    DNA synthesis of DNA pol-prim on natural ssDNA in the presence of RPA and PyV Tag. The cooperation of PyV Tag with mouse, human, and mutant pol-prims was tested on natural ssDNA bound by human RPA. In the reaction, ΦX174 ssDNA, RPA, and pol-prim complexes as indicated were incubated in the absence (shaded bars) or in the presence (solid bars) of PyV Tag. The incorporation of acid-insoluble dNMPs in the absence of Tag was arbitrarily set to 1. The presented data are the mean values and standard deviations of three experiments.
    Figure Legend Snippet: DNA synthesis of DNA pol-prim on natural ssDNA in the presence of RPA and PyV Tag. The cooperation of PyV Tag with mouse, human, and mutant pol-prims was tested on natural ssDNA bound by human RPA. In the reaction, ΦX174 ssDNA, RPA, and pol-prim complexes as indicated were incubated in the absence (shaded bars) or in the presence (solid bars) of PyV Tag. The incorporation of acid-insoluble dNMPs in the absence of Tag was arbitrarily set to 1. The presented data are the mean values and standard deviations of three experiments.

    Techniques Used: DNA Synthesis, Recombinase Polymerase Amplification, Mutagenesis, Incubation

    2) Product Images from "Ca2+ activates human homologous recombination protein Rad51 by modulating its ATPase activity"

    Article Title: Ca2+ activates human homologous recombination protein Rad51 by modulating its ATPase activity

    Journal:

    doi: 10.1073/pnas.0402105101

    Ca 2+ stimulates DNA three-strand exchange promoted by hRad51 protein. ( a ) The scheme and the time course of DNA stand exchange between φX174 circular ssDNA and linear dsDNA promoted by hRad51 protein analyzed in a 1% agarose gel. The reactions
    Figure Legend Snippet: Ca 2+ stimulates DNA three-strand exchange promoted by hRad51 protein. ( a ) The scheme and the time course of DNA stand exchange between φX174 circular ssDNA and linear dsDNA promoted by hRad51 protein analyzed in a 1% agarose gel. The reactions

    Techniques Used: Agarose Gel Electrophoresis

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    New England Biolabs φx174 ssdna
    DNA synthesis of DNA pol-prim on natural <t>ssDNA</t> in the presence of RPA and PyV Tag. The cooperation of PyV Tag with mouse, human, and mutant pol-prims was tested on natural ssDNA bound by human RPA. In the reaction, <t>ΦX174</t> ssDNA, RPA, and pol-prim complexes as indicated were incubated in the absence (shaded bars) or in the presence (solid bars) of PyV Tag. The incorporation of acid-insoluble dNMPs in the absence of Tag was arbitrarily set to 1. The presented data are the mean values and standard deviations of three experiments.
    φx174 Ssdna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/φx174 ssdna/product/New England Biolabs
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    φx174 ssdna - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

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    DNA synthesis of DNA pol-prim on natural ssDNA in the presence of RPA and PyV Tag. The cooperation of PyV Tag with mouse, human, and mutant pol-prims was tested on natural ssDNA bound by human RPA. In the reaction, ΦX174 ssDNA, RPA, and pol-prim complexes as indicated were incubated in the absence (shaded bars) or in the presence (solid bars) of PyV Tag. The incorporation of acid-insoluble dNMPs in the absence of Tag was arbitrarily set to 1. The presented data are the mean values and standard deviations of three experiments.

    Journal: Journal of Virology

    Article Title: Amino Acids 257 to 288 of Mouse p48 Control the Cooperation of Polyomavirus Large T Antigen, Replication Protein A, and DNA Polymerase ?-Primase To Synthesize DNA In Vitro

    doi: 10.1128/JVI.75.18.8569-8578.2001

    Figure Lengend Snippet: DNA synthesis of DNA pol-prim on natural ssDNA in the presence of RPA and PyV Tag. The cooperation of PyV Tag with mouse, human, and mutant pol-prims was tested on natural ssDNA bound by human RPA. In the reaction, ΦX174 ssDNA, RPA, and pol-prim complexes as indicated were incubated in the absence (shaded bars) or in the presence (solid bars) of PyV Tag. The incorporation of acid-insoluble dNMPs in the absence of Tag was arbitrarily set to 1. The presented data are the mean values and standard deviations of three experiments.

    Article Snippet: The DNA replication of ΦX174 ssDNA was carried out in a reaction mixture containing 66 ng of ΦX174 ssDNA (New England Biolabs); 20 mM Tris-acetate (pH 7.3); 5 mM magnesium acetate; 20 mM potassium acetate; 1 mM dithiothreitol; 0.1 mg of bovine serum albumin (BSA)/ml; 1 mM ATP; 0.1 mM (each) CTP, GTP, UTP, dATP, dCTP, dGTP, and TTP; and 0.1 mM [α-32 P]dCTP (Amersham Pharmacia Biotech) ( ).

    Techniques: DNA Synthesis, Recombinase Polymerase Amplification, Mutagenesis, Incubation

    Ca 2+ stimulates DNA three-strand exchange promoted by hRad51 protein. ( a ) The scheme and the time course of DNA stand exchange between φX174 circular ssDNA and linear dsDNA promoted by hRad51 protein analyzed in a 1% agarose gel. The reactions

    Journal:

    Article Title: Ca2+ activates human homologous recombination protein Rad51 by modulating its ATPase activity

    doi: 10.1073/pnas.0402105101

    Figure Lengend Snippet: Ca 2+ stimulates DNA three-strand exchange promoted by hRad51 protein. ( a ) The scheme and the time course of DNA stand exchange between φX174 circular ssDNA and linear dsDNA promoted by hRad51 protein analyzed in a 1% agarose gel. The reactions

    Article Snippet: Oligonucleotides, pUC19 DNA, and εDNA were prepared as described ( , ). φX174 ssDNA and dsDNA were purchased from New England Biolabs and Invitrogen, respectively.

    Techniques: Agarose Gel Electrophoresis

    Species-specific initiation of SV40 and BKV DNA replication by hybrid Pol-prim complexes. Increasing amounts (0.3 and 0.7 U of Pol-prim determined in the initiation assay on φX174 ssDNA template) of recombinant human (H4), mouse (M4), or hybrid

    Journal: Journal of Virology

    Article Title: Host-Specific Replication of BK Virus DNA in Mouse Cell Extracts Is Independently Controlled by DNA Polymerase ?-Primase and Inhibitory Activities ▿

    doi: 10.1128/JVI.00527-10

    Figure Lengend Snippet: Species-specific initiation of SV40 and BKV DNA replication by hybrid Pol-prim complexes. Increasing amounts (0.3 and 0.7 U of Pol-prim determined in the initiation assay on φX174 ssDNA template) of recombinant human (H4), mouse (M4), or hybrid

    Article Snippet: Briefly, the reaction mixture contained the following: 1 μg of φX174 ssDNA (NEB); 20 mM Tris-acetate (pH 7.3); 5 mM magnesium acetate; 20 mM potassium acetate; 1 mM dithiothreitol (DTT); 0.1 mg/ml BSA; 1 mM ATP; 0.1 mM each CTP, GTP, UTP, dATP, dCTP, dGTP, and dTTP; and 0.1 mM [α32 P]dCTP (3,000 Ci/mmol; Perkin-Elmer).

    Techniques: Recombinant

    DNA synthesis on φX174 ssDNA template by recombinant Pol-prim complexes. The recombinant four-subunit human and mouse Pol-prims (0.5 U per incubation) were incubated with φX174 ssDNA in the presence of recombinant purified RPA (1 μg)

    Journal: Journal of Virology

    Article Title: Host-Specific Replication of BK Virus DNA in Mouse Cell Extracts Is Independently Controlled by DNA Polymerase ?-Primase and Inhibitory Activities ▿

    doi: 10.1128/JVI.00527-10

    Figure Lengend Snippet: DNA synthesis on φX174 ssDNA template by recombinant Pol-prim complexes. The recombinant four-subunit human and mouse Pol-prims (0.5 U per incubation) were incubated with φX174 ssDNA in the presence of recombinant purified RPA (1 μg)

    Article Snippet: Briefly, the reaction mixture contained the following: 1 μg of φX174 ssDNA (NEB); 20 mM Tris-acetate (pH 7.3); 5 mM magnesium acetate; 20 mM potassium acetate; 1 mM dithiothreitol (DTT); 0.1 mg/ml BSA; 1 mM ATP; 0.1 mM each CTP, GTP, UTP, dATP, dCTP, dGTP, and dTTP; and 0.1 mM [α32 P]dCTP (3,000 Ci/mmol; Perkin-Elmer).

    Techniques: DNA Synthesis, Recombinant, Incubation, Purification, Recombinase Polymerase Amplification

    SsoRad54 protein stimulates SsoRadA-mediated DNA strand exchange. ( A ) Schematic of the DNA strand exchange reaction. ( B ) A time course of DNA strand exchange in the presence and absence of SsoRad54 protein at 80°C. Basal SsoRadA DNA strand exchange is shown over time in lanes 1, 3, 5, 7, 9 and 11. In lanes 2, 4, 6, 8, 10 and 12, SsoRad54 is added at the same time as SsoRadA, prior to the addition of linear φX174 dsDNA to the reaction. Lane 13 shows a reaction with SsoRad54 alone incubated for 60 min, while lane 14 shows a reaction lacking protein incubated for 90 min. JM indicates the position of joint molecules; NC represents the final nicked circular product, while dsDNA and ssDNA indicate the input DNA substrates. ( C ) A graphical representation of intermediate and product formation for data such as shown in (B), as well as results obtained from DNA strand exchange reactions conducted at 65°C. Reactions at 65°C that include SsoRad54 are shown with green triangles while those lacking SsoRad54 are represented by blue circles. Reactions at 80°C that include SsoRad54 are shown with red triangles while those lacking SsoRad54 are represented by orange circles. Utilization of the dsDNA substrate, and the formation of joint molecule intermediates and nicked circular dsDNA product are expressed as the percentage of dsDNA in each experiment. Error bars represent standard deviation between three replicate experiments.

    Journal: Nucleic Acids Research

    Article Title: An archaeal Rad54 protein remodels DNA and stimulates DNA strand exchange by RadA

    doi: 10.1093/nar/gkp068

    Figure Lengend Snippet: SsoRad54 protein stimulates SsoRadA-mediated DNA strand exchange. ( A ) Schematic of the DNA strand exchange reaction. ( B ) A time course of DNA strand exchange in the presence and absence of SsoRad54 protein at 80°C. Basal SsoRadA DNA strand exchange is shown over time in lanes 1, 3, 5, 7, 9 and 11. In lanes 2, 4, 6, 8, 10 and 12, SsoRad54 is added at the same time as SsoRadA, prior to the addition of linear φX174 dsDNA to the reaction. Lane 13 shows a reaction with SsoRad54 alone incubated for 60 min, while lane 14 shows a reaction lacking protein incubated for 90 min. JM indicates the position of joint molecules; NC represents the final nicked circular product, while dsDNA and ssDNA indicate the input DNA substrates. ( C ) A graphical representation of intermediate and product formation for data such as shown in (B), as well as results obtained from DNA strand exchange reactions conducted at 65°C. Reactions at 65°C that include SsoRad54 are shown with green triangles while those lacking SsoRad54 are represented by blue circles. Reactions at 80°C that include SsoRad54 are shown with red triangles while those lacking SsoRad54 are represented by orange circles. Utilization of the dsDNA substrate, and the formation of joint molecule intermediates and nicked circular dsDNA product are expressed as the percentage of dsDNA in each experiment. Error bars represent standard deviation between three replicate experiments.

    Article Snippet: DNA strand exchange reactions SsoRadA (11 μM) and SsoRad54 (16 nM, where indicated) were incubated with φX174 ssDNA (NEB) at a concentration of 33 μM (nucleotides) in 30 mM MES (pH 6.5), 15 mM Mg(OAc)2 , 2.5 mM ATP, 10 mM DTT, 5 μg/ml BSA at 80°C or 65°C for 10 min. After the addition of SsoSSB (7 μM), the reactions were incubated at 80°C or 65°C for another 5 min before introduction of φX174 dsDNA (NEB) at a concentration of 33 μM (nucleotides).

    Techniques: Incubation, Standard Deviation