λ dna template  (New England Biolabs)


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    Name:
    Lambda DNA
    Description:
    Lambda DNA 1 250 ug
    Catalog Number:
    n3011l
    Price:
    276
    Size:
    1 250 ug
    Category:
    Genomic DNA
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    Structured Review

    New England Biolabs λ dna template
    Lambda DNA
    Lambda DNA 1 250 ug
    https://www.bioz.com/result/λ dna template/product/New England Biolabs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    λ dna template - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Genomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis
    Article Snippet: Paragraph title: Cloning, DNA sequencing, and data analysis ... The size of the amplified DNA was compared to amplified Lambda kit control DNA, Lambda DNA (New England Biolabs (NEB), Ipswich, MA), and Hin d III-digested Lambda DNA (NEB).

    Centrifugation:

    Article Title: Genomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis
    Article Snippet: Cloning, DNA sequencing, and data analysis Fifty μl of dialyzed bacteriophage preparation obtained by centrifugation at 145,000 × g was treated with 0.5 units each of DNase and RNase for 30 min at 37°C, then 20 units of proteinase K (BRL) and 1% SDS for 1 h at 65°C. .. The size of the amplified DNA was compared to amplified Lambda kit control DNA, Lambda DNA (New England Biolabs (NEB), Ipswich, MA), and Hin d III-digested Lambda DNA (NEB).

    Amplification:

    Article Title: Genomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis
    Article Snippet: .. The size of the amplified DNA was compared to amplified Lambda kit control DNA, Lambda DNA (New England Biolabs (NEB), Ipswich, MA), and Hin d III-digested Lambda DNA (NEB). .. The amplified DNA was resolved by electrophoresis on a 0.8% agarose gel and DNA fragments between 2–23 kb were eluted from the gel using a Qiaquick kit (Qiagen).

    Agarose Gel Electrophoresis:

    Article Title: Genomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis
    Article Snippet: The size of the amplified DNA was compared to amplified Lambda kit control DNA, Lambda DNA (New England Biolabs (NEB), Ipswich, MA), and Hin d III-digested Lambda DNA (NEB). .. The amplified DNA was resolved by electrophoresis on a 0.8% agarose gel and DNA fragments between 2–23 kb were eluted from the gel using a Qiaquick kit (Qiagen).

    Article Title: A novel CRISPR/Cas9 associated technology for sequence-specific nucleic acid enrichment
    Article Snippet: .. Cas12a/crRNA followed by extension with phosphorothioated nucleotides protects lambda DNA from exonuclease digestion. (A) Diagram of lambda genomic DNA shows the relative positions of complexes Cas12a/crRNA Lambda R1 and Cas12a-crRNA Lambda F2: (B) A 0.7% agarose gel shows lambda DNA filled in with wild-type dNTPs (lane 1), lambda DNA filled in with wild-type dNTPs and incubated with exonuclease III (lane 2), lambda DNA filled in with phosphorothioated bases (dAαS lambda DNA) (lane 3), dAαS lambda DNA incubated with exonuclease III (lane 4), lambda DNA filled in with phosphorothioated bases (dGαS lambda DNA) (lane 5), dGαS lambda DNA incubated with exonuclease III (lane 6). .. Lambda DNA treated with Cas12a/crRNA and filled in with wild-type dNTPs (lane 7), lambda DNA filled in with wild-type dNTPs and incubated with exonuclease III (lane 8), lambda DNA filled in with phosphorothioated bases (dAαS lambda DNA) (lane 9), dAαS lambda DNA incubated with exonuclease III (lane 10), lambda DNA filled in with phosphorothioated bases (dGαS lambda DNA) (lane 11), dGαS lambda DNA incubated with exonuclease III (lane 12). (TIF) Click here for additional data file.

    Isolation:

    Article Title: Genomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis
    Article Snippet: The size of the amplified DNA was compared to amplified Lambda kit control DNA, Lambda DNA (New England Biolabs (NEB), Ipswich, MA), and Hin d III-digested Lambda DNA (NEB). .. Plasmid DNA was isolated using a QIAprep spin plasmid miniprep kit (Qiagen).

    Lambda DNA Preparation:

    Article Title: Genomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis
    Article Snippet: .. The size of the amplified DNA was compared to amplified Lambda kit control DNA, Lambda DNA (New England Biolabs (NEB), Ipswich, MA), and Hin d III-digested Lambda DNA (NEB). .. The amplified DNA was resolved by electrophoresis on a 0.8% agarose gel and DNA fragments between 2–23 kb were eluted from the gel using a Qiaquick kit (Qiagen).

    Article Title: A novel CRISPR/Cas9 associated technology for sequence-specific nucleic acid enrichment
    Article Snippet: .. Cas12a/crRNA followed by extension with phosphorothioated nucleotides protects lambda DNA from exonuclease digestion. (A) Diagram of lambda genomic DNA shows the relative positions of complexes Cas12a/crRNA Lambda R1 and Cas12a-crRNA Lambda F2: (B) A 0.7% agarose gel shows lambda DNA filled in with wild-type dNTPs (lane 1), lambda DNA filled in with wild-type dNTPs and incubated with exonuclease III (lane 2), lambda DNA filled in with phosphorothioated bases (dAαS lambda DNA) (lane 3), dAαS lambda DNA incubated with exonuclease III (lane 4), lambda DNA filled in with phosphorothioated bases (dGαS lambda DNA) (lane 5), dGαS lambda DNA incubated with exonuclease III (lane 6). .. Lambda DNA treated with Cas12a/crRNA and filled in with wild-type dNTPs (lane 7), lambda DNA filled in with wild-type dNTPs and incubated with exonuclease III (lane 8), lambda DNA filled in with phosphorothioated bases (dAαS lambda DNA) (lane 9), dAαS lambda DNA incubated with exonuclease III (lane 10), lambda DNA filled in with phosphorothioated bases (dGαS lambda DNA) (lane 11), dGαS lambda DNA incubated with exonuclease III (lane 12). (TIF) Click here for additional data file.

    Article Title: A novel CRISPR/Cas9 associated technology for sequence-specific nucleic acid enrichment
    Article Snippet: .. Lambda DNA treated with Cas12a/crRNA and filled in with wild-type dNTPs (lane 7), lambda DNA filled in with wild-type dNTPs and incubated with exonuclease III (lane 8), lambda DNA filled in with phosphorothioated bases (dAαS lambda DNA) (lane 9), dAαS lambda DNA incubated with exonuclease III (lane 10), lambda DNA filled in with phosphorothioated bases (dGαS lambda DNA) (lane 11), dGαS lambda DNA incubated with exonuclease III (lane 12). (TIF) Click here for additional data file. ..

    Electrophoresis:

    Article Title: Genomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis
    Article Snippet: The amplified product was heat-inactivated and precipitated with ethanol containing 0.15 M sodium acetate, 25 mM EDTA, reconstituted in buffer EB (Qiagen, Valencia, CA), and separated by electrophoresis on a 0.6% gel at 100 V for 60 min. .. The size of the amplified DNA was compared to amplified Lambda kit control DNA, Lambda DNA (New England Biolabs (NEB), Ipswich, MA), and Hin d III-digested Lambda DNA (NEB).

    Incubation:

    Article Title: A novel CRISPR/Cas9 associated technology for sequence-specific nucleic acid enrichment
    Article Snippet: .. Cas12a/crRNA followed by extension with phosphorothioated nucleotides protects lambda DNA from exonuclease digestion. (A) Diagram of lambda genomic DNA shows the relative positions of complexes Cas12a/crRNA Lambda R1 and Cas12a-crRNA Lambda F2: (B) A 0.7% agarose gel shows lambda DNA filled in with wild-type dNTPs (lane 1), lambda DNA filled in with wild-type dNTPs and incubated with exonuclease III (lane 2), lambda DNA filled in with phosphorothioated bases (dAαS lambda DNA) (lane 3), dAαS lambda DNA incubated with exonuclease III (lane 4), lambda DNA filled in with phosphorothioated bases (dGαS lambda DNA) (lane 5), dGαS lambda DNA incubated with exonuclease III (lane 6). .. Lambda DNA treated with Cas12a/crRNA and filled in with wild-type dNTPs (lane 7), lambda DNA filled in with wild-type dNTPs and incubated with exonuclease III (lane 8), lambda DNA filled in with phosphorothioated bases (dAαS lambda DNA) (lane 9), dAαS lambda DNA incubated with exonuclease III (lane 10), lambda DNA filled in with phosphorothioated bases (dGαS lambda DNA) (lane 11), dGαS lambda DNA incubated with exonuclease III (lane 12). (TIF) Click here for additional data file.

    Article Title: A novel CRISPR/Cas9 associated technology for sequence-specific nucleic acid enrichment
    Article Snippet: .. Lambda DNA treated with Cas12a/crRNA and filled in with wild-type dNTPs (lane 7), lambda DNA filled in with wild-type dNTPs and incubated with exonuclease III (lane 8), lambda DNA filled in with phosphorothioated bases (dAαS lambda DNA) (lane 9), dAαS lambda DNA incubated with exonuclease III (lane 10), lambda DNA filled in with phosphorothioated bases (dGαS lambda DNA) (lane 11), dGαS lambda DNA incubated with exonuclease III (lane 12). (TIF) Click here for additional data file. ..

    other:

    Article Title: Whole-Genome DNA Methylation Profile of the Jewel Wasp (Nasonia vitripennis)
    Article Snippet: We found that 99.28% of cytosines were converted in the lambda DNA.

    DNA Sequencing:

    Article Title: Genomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis
    Article Snippet: Paragraph title: Cloning, DNA sequencing, and data analysis ... The size of the amplified DNA was compared to amplified Lambda kit control DNA, Lambda DNA (New England Biolabs (NEB), Ipswich, MA), and Hin d III-digested Lambda DNA (NEB).

    Transformation Assay:

    Article Title: Genomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis
    Article Snippet: The size of the amplified DNA was compared to amplified Lambda kit control DNA, Lambda DNA (New England Biolabs (NEB), Ipswich, MA), and Hin d III-digested Lambda DNA (NEB). .. The gel-extracted bacteriophage fragments were cloned into pCR2.1 TOPO XL (Invitrogen) and transformed into chemically competent E. coli bacteriophage-resistant cells (One Shot Mach1-T1R ) (Invitrogen) using manufacture-recommended conditions (Invitrogen).

    Plasmid Preparation:

    Article Title: Genomic and proteomic characterization of SuMu, a Mu-like bacteriophage infecting Haemophilus parasuis
    Article Snippet: The size of the amplified DNA was compared to amplified Lambda kit control DNA, Lambda DNA (New England Biolabs (NEB), Ipswich, MA), and Hin d III-digested Lambda DNA (NEB). .. Plasmid DNA was isolated using a QIAprep spin plasmid miniprep kit (Qiagen).

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  • 95
    New England Biolabs ultra dna library prep kit
    MAB-seq strategy and quantitative mapping of active <t>DNA</t> demethylation ( a ) Schematic diagram of MAB-seq. DNMT methylates unmodified C to generate 5mC, which can be successively oxidized by TET to generate 5hmC/5fC/5caC. Highly oxidized cytosine derivatives, 5fC and 5caC, are repaired by TDG/BER to regenerate unmodified C. ( b ) M.SssI exhibits robust methylase activity towards unmodified cytosines within CpGs, but has significantly lower activity for cytosines in non-CpG contexts (CHG or CHH, H=A, T, C). M.SssI methylase activity was measured by MAB-seq analysis <t>(Illumina</t> deep sequencing) of unmethylated lambda DNA. Standard bisulfite sequencing (BS-seq) confirmed nearly complete conversion of unmethylated C to T at CpG and non-CpG sites. ( c ) Locus-specific MAB-seq analysis of 5fC/5caC at Tbx5 by Illumina sequencing in control ( shCtrl ) and Tdg knockdown ( shTdg ) mouse ESCs. For comparison, also shown are 5fC/5caC antibody DNA immunoprecipitation (DIP) based maps of 5fC and 5caC in control and Tdg -depleted mouse ESCs. DIP-seq tracks are represented in normalized read density (reads per 10 million reads, rp10m) and the vertical axis range of all DIP-seq tracks is from 1 to 25. Black horizontal bars denote 5fC/5caC-enriched regions identified by 5fC/5caC DIP-seq. The level of 5fC/5caC (only Watson strand shown) is displayed as the percentage of total C modified as 5fC/5caC, and background signals detected in Tet1/2 −/− mouse ESCs was subtracted. ( d ) Statistical calling of 5fC/5caC-modified CpGs in locus-specific MAB-seq analysis. Shown are 5fC/5caC levels (background corrected using raw MAB-seq signals in Tet1/2 −/− ) of 11 CpG sites from the Tbx5 locus in shCtrl, shCtrl +VC, shTdg and shTdg +VC mouse ESCs. shTdg was compared with shCtrl while shTdg +VC was compared with shCtrl +VC. An asterisk indicates that a CpG is statistically enriched for 5fC/5caC (multiple comparison corrected P
    Ultra Dna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultra dna library prep kit/product/New England Biolabs
    Average 95 stars, based on 176 article reviews
    Price from $9.99 to $1999.99
    ultra dna library prep kit - by Bioz Stars, 2020-02
    95/100 stars
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    95
    New England Biolabs lambda dna
    MAB-seq strategy and quantitative mapping of active <t>DNA</t> demethylation ( a ) Schematic diagram of MAB-seq. DNMT methylates unmodified C to generate 5mC, which can be successively oxidized by TET to generate 5hmC/5fC/5caC. Highly oxidized cytosine derivatives, 5fC and 5caC, are repaired by TDG/BER to regenerate unmodified C. ( b ) M.SssI exhibits robust methylase activity towards unmodified cytosines within CpGs, but has significantly lower activity for cytosines in non-CpG contexts (CHG or CHH, H=A, T, C). M.SssI methylase activity was measured by MAB-seq analysis <t>(Illumina</t> deep sequencing) of unmethylated lambda DNA. Standard bisulfite sequencing (BS-seq) confirmed nearly complete conversion of unmethylated C to T at CpG and non-CpG sites. ( c ) Locus-specific MAB-seq analysis of 5fC/5caC at Tbx5 by Illumina sequencing in control ( shCtrl ) and Tdg knockdown ( shTdg ) mouse ESCs. For comparison, also shown are 5fC/5caC antibody DNA immunoprecipitation (DIP) based maps of 5fC and 5caC in control and Tdg -depleted mouse ESCs. DIP-seq tracks are represented in normalized read density (reads per 10 million reads, rp10m) and the vertical axis range of all DIP-seq tracks is from 1 to 25. Black horizontal bars denote 5fC/5caC-enriched regions identified by 5fC/5caC DIP-seq. The level of 5fC/5caC (only Watson strand shown) is displayed as the percentage of total C modified as 5fC/5caC, and background signals detected in Tet1/2 −/− mouse ESCs was subtracted. ( d ) Statistical calling of 5fC/5caC-modified CpGs in locus-specific MAB-seq analysis. Shown are 5fC/5caC levels (background corrected using raw MAB-seq signals in Tet1/2 −/− ) of 11 CpG sites from the Tbx5 locus in shCtrl, shCtrl +VC, shTdg and shTdg +VC mouse ESCs. shTdg was compared with shCtrl while shTdg +VC was compared with shCtrl +VC. An asterisk indicates that a CpG is statistically enriched for 5fC/5caC (multiple comparison corrected P
    Lambda Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lambda dna/product/New England Biolabs
    Average 95 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    lambda dna - by Bioz Stars, 2020-02
    95/100 stars
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    79
    New England Biolabs λ dna templates
    MAB-seq strategy and quantitative mapping of active <t>DNA</t> demethylation ( a ) Schematic diagram of MAB-seq. DNMT methylates unmodified C to generate 5mC, which can be successively oxidized by TET to generate 5hmC/5fC/5caC. Highly oxidized cytosine derivatives, 5fC and 5caC, are repaired by TDG/BER to regenerate unmodified C. ( b ) M.SssI exhibits robust methylase activity towards unmodified cytosines within CpGs, but has significantly lower activity for cytosines in non-CpG contexts (CHG or CHH, H=A, T, C). M.SssI methylase activity was measured by MAB-seq analysis <t>(Illumina</t> deep sequencing) of unmethylated lambda DNA. Standard bisulfite sequencing (BS-seq) confirmed nearly complete conversion of unmethylated C to T at CpG and non-CpG sites. ( c ) Locus-specific MAB-seq analysis of 5fC/5caC at Tbx5 by Illumina sequencing in control ( shCtrl ) and Tdg knockdown ( shTdg ) mouse ESCs. For comparison, also shown are 5fC/5caC antibody DNA immunoprecipitation (DIP) based maps of 5fC and 5caC in control and Tdg -depleted mouse ESCs. DIP-seq tracks are represented in normalized read density (reads per 10 million reads, rp10m) and the vertical axis range of all DIP-seq tracks is from 1 to 25. Black horizontal bars denote 5fC/5caC-enriched regions identified by 5fC/5caC DIP-seq. The level of 5fC/5caC (only Watson strand shown) is displayed as the percentage of total C modified as 5fC/5caC, and background signals detected in Tet1/2 −/− mouse ESCs was subtracted. ( d ) Statistical calling of 5fC/5caC-modified CpGs in locus-specific MAB-seq analysis. Shown are 5fC/5caC levels (background corrected using raw MAB-seq signals in Tet1/2 −/− ) of 11 CpG sites from the Tbx5 locus in shCtrl, shCtrl +VC, shTdg and shTdg +VC mouse ESCs. shTdg was compared with shCtrl while shTdg +VC was compared with shCtrl +VC. An asterisk indicates that a CpG is statistically enriched for 5fC/5caC (multiple comparison corrected P
    λ Dna Templates, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/λ dna templates/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    λ dna templates - by Bioz Stars, 2020-02
    79/100 stars
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    MAB-seq strategy and quantitative mapping of active DNA demethylation ( a ) Schematic diagram of MAB-seq. DNMT methylates unmodified C to generate 5mC, which can be successively oxidized by TET to generate 5hmC/5fC/5caC. Highly oxidized cytosine derivatives, 5fC and 5caC, are repaired by TDG/BER to regenerate unmodified C. ( b ) M.SssI exhibits robust methylase activity towards unmodified cytosines within CpGs, but has significantly lower activity for cytosines in non-CpG contexts (CHG or CHH, H=A, T, C). M.SssI methylase activity was measured by MAB-seq analysis (Illumina deep sequencing) of unmethylated lambda DNA. Standard bisulfite sequencing (BS-seq) confirmed nearly complete conversion of unmethylated C to T at CpG and non-CpG sites. ( c ) Locus-specific MAB-seq analysis of 5fC/5caC at Tbx5 by Illumina sequencing in control ( shCtrl ) and Tdg knockdown ( shTdg ) mouse ESCs. For comparison, also shown are 5fC/5caC antibody DNA immunoprecipitation (DIP) based maps of 5fC and 5caC in control and Tdg -depleted mouse ESCs. DIP-seq tracks are represented in normalized read density (reads per 10 million reads, rp10m) and the vertical axis range of all DIP-seq tracks is from 1 to 25. Black horizontal bars denote 5fC/5caC-enriched regions identified by 5fC/5caC DIP-seq. The level of 5fC/5caC (only Watson strand shown) is displayed as the percentage of total C modified as 5fC/5caC, and background signals detected in Tet1/2 −/− mouse ESCs was subtracted. ( d ) Statistical calling of 5fC/5caC-modified CpGs in locus-specific MAB-seq analysis. Shown are 5fC/5caC levels (background corrected using raw MAB-seq signals in Tet1/2 −/− ) of 11 CpG sites from the Tbx5 locus in shCtrl, shCtrl +VC, shTdg and shTdg +VC mouse ESCs. shTdg was compared with shCtrl while shTdg +VC was compared with shCtrl +VC. An asterisk indicates that a CpG is statistically enriched for 5fC/5caC (multiple comparison corrected P

    Journal: Nature biotechnology

    Article Title: Single-base resolution analysis of active DNA demethylation using methylase-assisted bisulfite sequencing

    doi: 10.1038/nbt.3073

    Figure Lengend Snippet: MAB-seq strategy and quantitative mapping of active DNA demethylation ( a ) Schematic diagram of MAB-seq. DNMT methylates unmodified C to generate 5mC, which can be successively oxidized by TET to generate 5hmC/5fC/5caC. Highly oxidized cytosine derivatives, 5fC and 5caC, are repaired by TDG/BER to regenerate unmodified C. ( b ) M.SssI exhibits robust methylase activity towards unmodified cytosines within CpGs, but has significantly lower activity for cytosines in non-CpG contexts (CHG or CHH, H=A, T, C). M.SssI methylase activity was measured by MAB-seq analysis (Illumina deep sequencing) of unmethylated lambda DNA. Standard bisulfite sequencing (BS-seq) confirmed nearly complete conversion of unmethylated C to T at CpG and non-CpG sites. ( c ) Locus-specific MAB-seq analysis of 5fC/5caC at Tbx5 by Illumina sequencing in control ( shCtrl ) and Tdg knockdown ( shTdg ) mouse ESCs. For comparison, also shown are 5fC/5caC antibody DNA immunoprecipitation (DIP) based maps of 5fC and 5caC in control and Tdg -depleted mouse ESCs. DIP-seq tracks are represented in normalized read density (reads per 10 million reads, rp10m) and the vertical axis range of all DIP-seq tracks is from 1 to 25. Black horizontal bars denote 5fC/5caC-enriched regions identified by 5fC/5caC DIP-seq. The level of 5fC/5caC (only Watson strand shown) is displayed as the percentage of total C modified as 5fC/5caC, and background signals detected in Tet1/2 −/− mouse ESCs was subtracted. ( d ) Statistical calling of 5fC/5caC-modified CpGs in locus-specific MAB-seq analysis. Shown are 5fC/5caC levels (background corrected using raw MAB-seq signals in Tet1/2 −/− ) of 11 CpG sites from the Tbx5 locus in shCtrl, shCtrl +VC, shTdg and shTdg +VC mouse ESCs. shTdg was compared with shCtrl while shTdg +VC was compared with shCtrl +VC. An asterisk indicates that a CpG is statistically enriched for 5fC/5caC (multiple comparison corrected P

    Article Snippet: Library generation for genome-scale MAB-seq/BS-seq 1 ug unenriched genomic DNA (whole-genome) or 200–500 ng of ChIP DNA (H3K4me1- or H3K27me3-enriched) was first spiked-in with unmethylated lambda DNA (1:400), which was then repaired and ligated to methylated (5mC) custom adapters (forward 5’-ACACTCTTTCCCTACACGACGCTCTTCC GATC*T-3’; reverse 5’-/5Phos/GATCGGAAGAGCACACGTCTGAACTCCAGTC-3’; the asterisk denotes phosphorothioate bond) with the NEBNext Ultra DNA Library Prep kit from Illumina (NEB).

    Techniques: Activity Assay, Sequencing, Lambda DNA Preparation, Methylation Sequencing, Immunoprecipitation, DNA Immunoprecipitation Sequencing, Modification