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Santa Cruz Biotechnology β glycerophosphate
ADAMTS7 reinforced osteogenic differentiation and suppressed OS pathogenesis via BMP2. (A, B) Representative images of biomineralization by alizarin red staining in MG63 cells stimulated by ascorbic acid and <t>β‐glycerophosphate</t> for 21 days with ADAMTS7 overexpressing (A) or silencing (B). Calcium deposition was analyzed and quantified as the mean ± SEM ( n = 3). * P
β Glycerophosphate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "ADAMTS7 degrades Comp to fuel BMP2‐dependent osteogenic differentiation and ameliorate oncogenic potential in osteosarcomas"

Article Title: ADAMTS7 degrades Comp to fuel BMP2‐dependent osteogenic differentiation and ameliorate oncogenic potential in osteosarcomas

Journal: FEBS Open Bio

doi: 10.1002/2211-5463.12939

ADAMTS7 reinforced osteogenic differentiation and suppressed OS pathogenesis via BMP2. (A, B) Representative images of biomineralization by alizarin red staining in MG63 cells stimulated by ascorbic acid and β‐glycerophosphate for 21 days with ADAMTS7 overexpressing (A) or silencing (B). Calcium deposition was analyzed and quantified as the mean ± SEM ( n = 3). * P
Figure Legend Snippet: ADAMTS7 reinforced osteogenic differentiation and suppressed OS pathogenesis via BMP2. (A, B) Representative images of biomineralization by alizarin red staining in MG63 cells stimulated by ascorbic acid and β‐glycerophosphate for 21 days with ADAMTS7 overexpressing (A) or silencing (B). Calcium deposition was analyzed and quantified as the mean ± SEM ( n = 3). * P

Techniques Used: Staining

Related Articles

Transfection:

Article Title: Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿ †
Article Snippet: .. After transfection or treatment, 200 to 500 μg of whole-cell extracts of 293T, HeLa, or EREV8 cells was prepared in lysis buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 5 mM EDTA [pH 8.0], 30 mM NaF, 1 mM Na3 VO4 , 40 mM β-glycerophosphate, 1× protease inhibitor mixture, 10% glycerol, and 1% NP-40) and then incubated overnight with 1 μg of EBNA-1 5C11 monoclonal antibody (MAb) , anti-HA MAb, anti-Flag MAb, anti-BGLF4 (2224) , or anti-CBP MAb (Santa Cruz Biotechnology). .. The mixtures were then incubated with 100 μl (20%) of a 1:1 slurry of protein A/G-, protein A-, or protein G-Sepharose beads (Amersham Biosciences) for 2 h at 4°C.

Protease Inhibitor:

Article Title: NBR1 enables autophagy-dependent focal adhesion turnover
Article Snippet: .. For immunoprecipitation, cells expressing GFP or GFP-NBR1 were sparsely plated on fibronectin-coated (10 µg/ml in PBS) dishes and were lysed in nondenaturing lysis buffer (1% Triton X-100, 25 mM Tris HCl, pH 7.4, and 150 mM NaCl) plus protease inhibitor cocktail (Sigma-Aldrich), 10 mM N-ethylmaleimide, 10 mM NaF, 10 mM β-glycerophosphate, 1 mM Na3 VO4 , 10 nM calyculin A, 0.5 mM PMSF, 10 µg/ml E64d, and 10 µg/ml pepstatin A. Lysates were precleared with protein A/G beads (Santa Cruz Biotechnology, Inc.) and normal rabbit IgG (Santa Cruz Biotechnology, Inc.) at 4°C and incubated overnight with rabbit anti-GFP primary antibody (ab6556; Abcam; 1 µg/200–300 µg lysate) at 4°C. .. Immune complexes were captured by incubation with protein A/G beads for 4 h at 4°C and then washed six times wi th PBS plus inhibitors, eluted with sample buffer, and analyzed by immunoblotting.

Article Title: Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿ †
Article Snippet: .. After transfection or treatment, 200 to 500 μg of whole-cell extracts of 293T, HeLa, or EREV8 cells was prepared in lysis buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 5 mM EDTA [pH 8.0], 30 mM NaF, 1 mM Na3 VO4 , 40 mM β-glycerophosphate, 1× protease inhibitor mixture, 10% glycerol, and 1% NP-40) and then incubated overnight with 1 μg of EBNA-1 5C11 monoclonal antibody (MAb) , anti-HA MAb, anti-Flag MAb, anti-BGLF4 (2224) , or anti-CBP MAb (Santa Cruz Biotechnology). .. The mixtures were then incubated with 100 μl (20%) of a 1:1 slurry of protein A/G-, protein A-, or protein G-Sepharose beads (Amersham Biosciences) for 2 h at 4°C.

Immunoprecipitation:

Article Title: NBR1 enables autophagy-dependent focal adhesion turnover
Article Snippet: .. For immunoprecipitation, cells expressing GFP or GFP-NBR1 were sparsely plated on fibronectin-coated (10 µg/ml in PBS) dishes and were lysed in nondenaturing lysis buffer (1% Triton X-100, 25 mM Tris HCl, pH 7.4, and 150 mM NaCl) plus protease inhibitor cocktail (Sigma-Aldrich), 10 mM N-ethylmaleimide, 10 mM NaF, 10 mM β-glycerophosphate, 1 mM Na3 VO4 , 10 nM calyculin A, 0.5 mM PMSF, 10 µg/ml E64d, and 10 µg/ml pepstatin A. Lysates were precleared with protein A/G beads (Santa Cruz Biotechnology, Inc.) and normal rabbit IgG (Santa Cruz Biotechnology, Inc.) at 4°C and incubated overnight with rabbit anti-GFP primary antibody (ab6556; Abcam; 1 µg/200–300 µg lysate) at 4°C. .. Immune complexes were captured by incubation with protein A/G beads for 4 h at 4°C and then washed six times wi th PBS plus inhibitors, eluted with sample buffer, and analyzed by immunoblotting.

Article Title: Calcium/Calcineurin Synergizes with Prostratin to Promote NF-?B Dependent Activation of Latent HIV
Article Snippet: .. At each time point, 5 million cells were lysed in whole-cell extract lysis buffer (see above) supplemented with 1 mM DTT, 1 mM PMSF, 10 mM p -nitrophenyl phosphate, 10 mM β-glycerophosphate, 200 µM sodium vanadate, 10 µg/ml aprotinin, and 1 µg/ml pepstatin and immunoprecipitated with antibodies against IκB kinase (IKK)-α(sc-7218, Santa Cruz Biotechnology). .. In vitro kinase assays using glutathione S-transferase IκBα (1–62) as the substrate were performed as described .

Article Title: ULK1 inhibits mTORC1 signaling, promotes multisite Raptor phosphorylation and hinders substrate binding
Article Snippet: .. Cells for ULK1 immunoprecipitation were lysed in Buffer B (40 mM HEPES (pH 7.5), 120 mM NaCl, 1 mM EDTA, 10 mM pyrophosphate, 10 mM β-glycerophosphate, 50 mM NaF, 1.5 mM Na3 VO4 , 0.3% (w/v) CHAPS plus protease inhibitors) and immunoprecipitated using anti-ULK1 antibody (Santa Cruz) and Protein G-Sepharose. .. Beads were washed three times in Buffer B prior to addition of sample buffer (Invitrogen).

Article Title: Constitutive activation of NF-?B and T-cell leukemia/lymphoma in Notch3 transgenic mice
Article Snippet: .. Cell lysates in KLB [kinase lysis buffer; 50 mM Tris pH 7.5, 250 mM NaCl, 1 mM EGTA, 1 mM EDTA, 1% Triton X-100, 0.5% NP-40, 10% glycerol, 0.5 mM dithiothreitol (DTT), 20 mM β-glycerophosphate, 10 mM NaF, 1 mM sodium vanadate and 0.5 mM phenylmethylsulfonyl fluoride (PMSF) on ice for 30 min] were immunoprecipitated with anti-IKKα (M-280) and protein A/G plus agarose (Santa Cruz Biotech) for 2 h at 4°C, washed with 400 mM NaCl/2 M urea KLB, then with 20 mM HEPES pH 7.5, 10 mM MgCl2 , 20 mM β-glycerophosphate, 10 mM NaF, 1 mM NaH2 PO4 , 2 mM DTT, and then incubated with 5 µCi of [γ-32 P]ATP and 5 µg of recombinant GST–IκBα (1–317) (Santa Cruz Biotech) at 30°C for 30 min. .. The samples were analyzed by SDS–PAGE, transferred to nitrocellulose membranes and subsequently probed with anti-IKKα antibody.

Incubation:

Article Title: NBR1 enables autophagy-dependent focal adhesion turnover
Article Snippet: .. For immunoprecipitation, cells expressing GFP or GFP-NBR1 were sparsely plated on fibronectin-coated (10 µg/ml in PBS) dishes and were lysed in nondenaturing lysis buffer (1% Triton X-100, 25 mM Tris HCl, pH 7.4, and 150 mM NaCl) plus protease inhibitor cocktail (Sigma-Aldrich), 10 mM N-ethylmaleimide, 10 mM NaF, 10 mM β-glycerophosphate, 1 mM Na3 VO4 , 10 nM calyculin A, 0.5 mM PMSF, 10 µg/ml E64d, and 10 µg/ml pepstatin A. Lysates were precleared with protein A/G beads (Santa Cruz Biotechnology, Inc.) and normal rabbit IgG (Santa Cruz Biotechnology, Inc.) at 4°C and incubated overnight with rabbit anti-GFP primary antibody (ab6556; Abcam; 1 µg/200–300 µg lysate) at 4°C. .. Immune complexes were captured by incubation with protein A/G beads for 4 h at 4°C and then washed six times wi th PBS plus inhibitors, eluted with sample buffer, and analyzed by immunoblotting.

Article Title: Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿ †
Article Snippet: .. After transfection or treatment, 200 to 500 μg of whole-cell extracts of 293T, HeLa, or EREV8 cells was prepared in lysis buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 5 mM EDTA [pH 8.0], 30 mM NaF, 1 mM Na3 VO4 , 40 mM β-glycerophosphate, 1× protease inhibitor mixture, 10% glycerol, and 1% NP-40) and then incubated overnight with 1 μg of EBNA-1 5C11 monoclonal antibody (MAb) , anti-HA MAb, anti-Flag MAb, anti-BGLF4 (2224) , or anti-CBP MAb (Santa Cruz Biotechnology). .. The mixtures were then incubated with 100 μl (20%) of a 1:1 slurry of protein A/G-, protein A-, or protein G-Sepharose beads (Amersham Biosciences) for 2 h at 4°C.

Article Title: Constitutive activation of NF-?B and T-cell leukemia/lymphoma in Notch3 transgenic mice
Article Snippet: .. Cell lysates in KLB [kinase lysis buffer; 50 mM Tris pH 7.5, 250 mM NaCl, 1 mM EGTA, 1 mM EDTA, 1% Triton X-100, 0.5% NP-40, 10% glycerol, 0.5 mM dithiothreitol (DTT), 20 mM β-glycerophosphate, 10 mM NaF, 1 mM sodium vanadate and 0.5 mM phenylmethylsulfonyl fluoride (PMSF) on ice for 30 min] were immunoprecipitated with anti-IKKα (M-280) and protein A/G plus agarose (Santa Cruz Biotech) for 2 h at 4°C, washed with 400 mM NaCl/2 M urea KLB, then with 20 mM HEPES pH 7.5, 10 mM MgCl2 , 20 mM β-glycerophosphate, 10 mM NaF, 1 mM NaH2 PO4 , 2 mM DTT, and then incubated with 5 µCi of [γ-32 P]ATP and 5 µg of recombinant GST–IκBα (1–317) (Santa Cruz Biotech) at 30°C for 30 min. .. The samples were analyzed by SDS–PAGE, transferred to nitrocellulose membranes and subsequently probed with anti-IKKα antibody.

Expressing:

Article Title: NBR1 enables autophagy-dependent focal adhesion turnover
Article Snippet: .. For immunoprecipitation, cells expressing GFP or GFP-NBR1 were sparsely plated on fibronectin-coated (10 µg/ml in PBS) dishes and were lysed in nondenaturing lysis buffer (1% Triton X-100, 25 mM Tris HCl, pH 7.4, and 150 mM NaCl) plus protease inhibitor cocktail (Sigma-Aldrich), 10 mM N-ethylmaleimide, 10 mM NaF, 10 mM β-glycerophosphate, 1 mM Na3 VO4 , 10 nM calyculin A, 0.5 mM PMSF, 10 µg/ml E64d, and 10 µg/ml pepstatin A. Lysates were precleared with protein A/G beads (Santa Cruz Biotechnology, Inc.) and normal rabbit IgG (Santa Cruz Biotechnology, Inc.) at 4°C and incubated overnight with rabbit anti-GFP primary antibody (ab6556; Abcam; 1 µg/200–300 µg lysate) at 4°C. .. Immune complexes were captured by incubation with protein A/G beads for 4 h at 4°C and then washed six times wi th PBS plus inhibitors, eluted with sample buffer, and analyzed by immunoblotting.

Lysis:

Article Title: NBR1 enables autophagy-dependent focal adhesion turnover
Article Snippet: .. For immunoprecipitation, cells expressing GFP or GFP-NBR1 were sparsely plated on fibronectin-coated (10 µg/ml in PBS) dishes and were lysed in nondenaturing lysis buffer (1% Triton X-100, 25 mM Tris HCl, pH 7.4, and 150 mM NaCl) plus protease inhibitor cocktail (Sigma-Aldrich), 10 mM N-ethylmaleimide, 10 mM NaF, 10 mM β-glycerophosphate, 1 mM Na3 VO4 , 10 nM calyculin A, 0.5 mM PMSF, 10 µg/ml E64d, and 10 µg/ml pepstatin A. Lysates were precleared with protein A/G beads (Santa Cruz Biotechnology, Inc.) and normal rabbit IgG (Santa Cruz Biotechnology, Inc.) at 4°C and incubated overnight with rabbit anti-GFP primary antibody (ab6556; Abcam; 1 µg/200–300 µg lysate) at 4°C. .. Immune complexes were captured by incubation with protein A/G beads for 4 h at 4°C and then washed six times wi th PBS plus inhibitors, eluted with sample buffer, and analyzed by immunoblotting.

Article Title: Calcium/Calcineurin Synergizes with Prostratin to Promote NF-?B Dependent Activation of Latent HIV
Article Snippet: .. At each time point, 5 million cells were lysed in whole-cell extract lysis buffer (see above) supplemented with 1 mM DTT, 1 mM PMSF, 10 mM p -nitrophenyl phosphate, 10 mM β-glycerophosphate, 200 µM sodium vanadate, 10 µg/ml aprotinin, and 1 µg/ml pepstatin and immunoprecipitated with antibodies against IκB kinase (IKK)-α(sc-7218, Santa Cruz Biotechnology). .. In vitro kinase assays using glutathione S-transferase IκBα (1–62) as the substrate were performed as described .

Article Title: Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿Epstein-Barr Virus BGLF4 Kinase Suppresses the Interferon Regulatory Factor 3 Signaling Pathway ▿ †
Article Snippet: .. After transfection or treatment, 200 to 500 μg of whole-cell extracts of 293T, HeLa, or EREV8 cells was prepared in lysis buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 5 mM EDTA [pH 8.0], 30 mM NaF, 1 mM Na3 VO4 , 40 mM β-glycerophosphate, 1× protease inhibitor mixture, 10% glycerol, and 1% NP-40) and then incubated overnight with 1 μg of EBNA-1 5C11 monoclonal antibody (MAb) , anti-HA MAb, anti-Flag MAb, anti-BGLF4 (2224) , or anti-CBP MAb (Santa Cruz Biotechnology). .. The mixtures were then incubated with 100 μl (20%) of a 1:1 slurry of protein A/G-, protein A-, or protein G-Sepharose beads (Amersham Biosciences) for 2 h at 4°C.

Article Title: Constitutive activation of NF-?B and T-cell leukemia/lymphoma in Notch3 transgenic mice
Article Snippet: .. Cell lysates in KLB [kinase lysis buffer; 50 mM Tris pH 7.5, 250 mM NaCl, 1 mM EGTA, 1 mM EDTA, 1% Triton X-100, 0.5% NP-40, 10% glycerol, 0.5 mM dithiothreitol (DTT), 20 mM β-glycerophosphate, 10 mM NaF, 1 mM sodium vanadate and 0.5 mM phenylmethylsulfonyl fluoride (PMSF) on ice for 30 min] were immunoprecipitated with anti-IKKα (M-280) and protein A/G plus agarose (Santa Cruz Biotech) for 2 h at 4°C, washed with 400 mM NaCl/2 M urea KLB, then with 20 mM HEPES pH 7.5, 10 mM MgCl2 , 20 mM β-glycerophosphate, 10 mM NaF, 1 mM NaH2 PO4 , 2 mM DTT, and then incubated with 5 µCi of [γ-32 P]ATP and 5 µg of recombinant GST–IκBα (1–317) (Santa Cruz Biotech) at 30°C for 30 min. .. The samples were analyzed by SDS–PAGE, transferred to nitrocellulose membranes and subsequently probed with anti-IKKα antibody.

Staining:

Article Title: ADAMTS7 degrades Comp to fuel BMP2‐dependent osteogenic differentiation and ameliorate oncogenic potential in osteosarcomas
Article Snippet: .. Alizarin red staining MG63 cells were stimulated by 0.5 mm β‐glycerophosphate (#sc‐220452; Santa Cruz Biotechnology) and 50 mg·L−1 l‐ascorbic acid (#A92902; Sigma‐Aldrich, St Louis, MO, USA) for 21 days to induce osteogenic differentiation. .. Cells were rinsed with PBS for three times, fixed with formalin for 30 min, and stained with alizarin red S (#A5533; Sigma‐Aldrich) for 15 min and washed with 0.2% acetic acid.

Recombinant:

Article Title: Constitutive activation of NF-?B and T-cell leukemia/lymphoma in Notch3 transgenic mice
Article Snippet: .. Cell lysates in KLB [kinase lysis buffer; 50 mM Tris pH 7.5, 250 mM NaCl, 1 mM EGTA, 1 mM EDTA, 1% Triton X-100, 0.5% NP-40, 10% glycerol, 0.5 mM dithiothreitol (DTT), 20 mM β-glycerophosphate, 10 mM NaF, 1 mM sodium vanadate and 0.5 mM phenylmethylsulfonyl fluoride (PMSF) on ice for 30 min] were immunoprecipitated with anti-IKKα (M-280) and protein A/G plus agarose (Santa Cruz Biotech) for 2 h at 4°C, washed with 400 mM NaCl/2 M urea KLB, then with 20 mM HEPES pH 7.5, 10 mM MgCl2 , 20 mM β-glycerophosphate, 10 mM NaF, 1 mM NaH2 PO4 , 2 mM DTT, and then incubated with 5 µCi of [γ-32 P]ATP and 5 µg of recombinant GST–IκBα (1–317) (Santa Cruz Biotech) at 30°C for 30 min. .. The samples were analyzed by SDS–PAGE, transferred to nitrocellulose membranes and subsequently probed with anti-IKKα antibody.

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    Santa Cruz Biotechnology β glycerophosphate
    ADAMTS7 reinforced osteogenic differentiation and suppressed OS pathogenesis via BMP2. (A, B) Representative images of biomineralization by alizarin red staining in MG63 cells stimulated by ascorbic acid and <t>β‐glycerophosphate</t> for 21 days with ADAMTS7 overexpressing (A) or silencing (B). Calcium deposition was analyzed and quantified as the mean ± SEM ( n = 3). * P
    β Glycerophosphate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Santa Cruz Biotechnology
    Average 93 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    β glycerophosphate - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    91
    Santa Cruz Biotechnology sodium β glycerophosphate
    ADAMTS7 reinforced osteogenic differentiation and suppressed OS pathogenesis via BMP2. (A, B) Representative images of biomineralization by alizarin red staining in MG63 cells stimulated by ascorbic acid and <t>β‐glycerophosphate</t> for 21 days with ADAMTS7 overexpressing (A) or silencing (B). Calcium deposition was analyzed and quantified as the mean ± SEM ( n = 3). * P
    Sodium β Glycerophosphate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium β glycerophosphate/product/Santa Cruz Biotechnology
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    sodium β glycerophosphate - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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    ADAMTS7 reinforced osteogenic differentiation and suppressed OS pathogenesis via BMP2. (A, B) Representative images of biomineralization by alizarin red staining in MG63 cells stimulated by ascorbic acid and β‐glycerophosphate for 21 days with ADAMTS7 overexpressing (A) or silencing (B). Calcium deposition was analyzed and quantified as the mean ± SEM ( n = 3). * P

    Journal: FEBS Open Bio

    Article Title: ADAMTS7 degrades Comp to fuel BMP2‐dependent osteogenic differentiation and ameliorate oncogenic potential in osteosarcomas

    doi: 10.1002/2211-5463.12939

    Figure Lengend Snippet: ADAMTS7 reinforced osteogenic differentiation and suppressed OS pathogenesis via BMP2. (A, B) Representative images of biomineralization by alizarin red staining in MG63 cells stimulated by ascorbic acid and β‐glycerophosphate for 21 days with ADAMTS7 overexpressing (A) or silencing (B). Calcium deposition was analyzed and quantified as the mean ± SEM ( n = 3). * P

    Article Snippet: Alizarin red staining MG63 cells were stimulated by 0.5 mm β‐glycerophosphate (#sc‐220452; Santa Cruz Biotechnology) and 50 mg·L−1 l‐ascorbic acid (#A92902; Sigma‐Aldrich, St Louis, MO, USA) for 21 days to induce osteogenic differentiation.

    Techniques: Staining