Structured Review

Merck KGaA β glycerophosphate
Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
β Glycerophosphate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β glycerophosphate/product/Merck KGaA
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
β glycerophosphate - by Bioz Stars, 2020-09
92/100 stars

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1) Product Images from "Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation"

Article Title: Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation

Journal: Scientific Reports

doi: 10.1038/s41598-018-20241-3

Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
Figure Legend Snippet: Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+ cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+ supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+ supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP, β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.

Techniques Used: Cell Culture, Isolation, Spectroscopy

Impact of Mg 2+  on BGP-induced Ca 2+ -apatite formation. X-ray powder diffraction analysis of cell culture supernatants show presence of NaCl (*) and Ca-apatite (arrows) in BGP treated cultures (black line), but not in BGP cultures supplemented with Mg 2+  (gray line). Of note: as the BGP culture supplemented with Mg 2+  contained less precipitates; one measurement represents 21 cultures (6-well format) compared to 7 in the BGP condition in order to reach the detection threshold. BGP,  β -glycerophosphate.
Figure Legend Snippet: Impact of Mg 2+ on BGP-induced Ca 2+ -apatite formation. X-ray powder diffraction analysis of cell culture supernatants show presence of NaCl (*) and Ca-apatite (arrows) in BGP treated cultures (black line), but not in BGP cultures supplemented with Mg 2+ (gray line). Of note: as the BGP culture supplemented with Mg 2+ contained less precipitates; one measurement represents 21 cultures (6-well format) compared to 7 in the BGP condition in order to reach the detection threshold. BGP, β -glycerophosphate.

Techniques Used: Cell Culture

2) Product Images from "Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation"

Article Title: Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation

Journal: Scientific Reports

doi: 10.1038/s41598-018-20241-3

Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
Figure Legend Snippet: Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+ cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+ supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+ supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP, β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.

Techniques Used: Cell Culture, Isolation, Spectroscopy

Impact of Mg 2+  on BGP-induced Ca 2+ -apatite formation. X-ray powder diffraction analysis of cell culture supernatants show presence of NaCl (*) and Ca-apatite (arrows) in BGP treated cultures (black line), but not in BGP cultures supplemented with Mg 2+  (gray line). Of note: as the BGP culture supplemented with Mg 2+  contained less precipitates; one measurement represents 21 cultures (6-well format) compared to 7 in the BGP condition in order to reach the detection threshold. BGP,  β -glycerophosphate.
Figure Legend Snippet: Impact of Mg 2+ on BGP-induced Ca 2+ -apatite formation. X-ray powder diffraction analysis of cell culture supernatants show presence of NaCl (*) and Ca-apatite (arrows) in BGP treated cultures (black line), but not in BGP cultures supplemented with Mg 2+ (gray line). Of note: as the BGP culture supplemented with Mg 2+ contained less precipitates; one measurement represents 21 cultures (6-well format) compared to 7 in the BGP condition in order to reach the detection threshold. BGP, β -glycerophosphate.

Techniques Used: Cell Culture

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Protease Inhibitor:

Article Title: Time-series proteomic study of the response of HK-2 cells to hyperglycemic, hypoxic diabetic-like milieu
Article Snippet: .. Ammonia solution and NaCl were obtained from Merck (Darmstadt, Germany). β-glycerophosphate, DTT, EDTA, formaldehyde (CH2 O) (37%, v/v), iodoacetamide (IAA), n-octyl-β-d-glucopyranoside (BOG), PBS, protease inhibitor cocktail, sodium cyanoborohydride (NaBH3 CN), sodium fluoride (NaF), sodium metavanadate (NaVO4 ), tributylphosphate, triethylammonium bicarbonate (TEAB) and TFA were purchased from Sigma-Aldrich (St. Louis, MO). .. Trypsin/Lys-C mix (Mass Spec grade V5072) was purchased from Promega (Madison, WI), deuterated formaldehyde (CD2O) (20%, v/v) was obtained from ISOTEC (Miamisburg, OH) and sodium pyrophosphate from Alfa Aesar (Kandel, Germany).

ALP Activity Assay:

Article Title: TAFA2 Induces Skeletal (Stromal) Stem Cell Migration Through Activation of Rac1‐p38 Signaling
Article Snippet: .. Osteoblast Differentiation and ALP Activity Assay Osteogenic induction was performed using MEM supplemented with 10 mM β‐glycerophosphate (Calbiochem‐Merck, Germany), 50 μg/ml l ‐ascorbic acid‐2‐phosphate (Wako Chemicals GmbH, Germany), 10 nM dexamethasone (Sigma‐Aldrich, Denmark), and 10 nM calcitriol (1.25‐dihydroxy vitamin‐D3) kindly provided by Leo Pharma, Ballerup, Denmark. ..

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    Merck KGaA β glycerophosphate
    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.
    β Glycerophosphate, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Merck KGaA
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    β glycerophosphate - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.

    Journal: Scientific Reports

    Article Title: Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation

    doi: 10.1038/s41598-018-20241-3

    Figure Lengend Snippet: Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+ cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+ supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+ supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP, β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.

    Article Snippet: Experimental design For calcification, medium was supplemented with 5% (v/v) FBS and 10 mM β -glycerophosphate (BGP, Merck Millipore, Massachusetts, USA).

    Techniques: Cell Culture, Isolation, Spectroscopy

    Impact of Mg 2+  on BGP-induced Ca 2+ -apatite formation. X-ray powder diffraction analysis of cell culture supernatants show presence of NaCl (*) and Ca-apatite (arrows) in BGP treated cultures (black line), but not in BGP cultures supplemented with Mg 2+  (gray line). Of note: as the BGP culture supplemented with Mg 2+  contained less precipitates; one measurement represents 21 cultures (6-well format) compared to 7 in the BGP condition in order to reach the detection threshold. BGP,  β -glycerophosphate.

    Journal: Scientific Reports

    Article Title: Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation

    doi: 10.1038/s41598-018-20241-3

    Figure Lengend Snippet: Impact of Mg 2+ on BGP-induced Ca 2+ -apatite formation. X-ray powder diffraction analysis of cell culture supernatants show presence of NaCl (*) and Ca-apatite (arrows) in BGP treated cultures (black line), but not in BGP cultures supplemented with Mg 2+ (gray line). Of note: as the BGP culture supplemented with Mg 2+ contained less precipitates; one measurement represents 21 cultures (6-well format) compared to 7 in the BGP condition in order to reach the detection threshold. BGP, β -glycerophosphate.

    Article Snippet: Experimental design For calcification, medium was supplemented with 5% (v/v) FBS and 10 mM β -glycerophosphate (BGP, Merck Millipore, Massachusetts, USA).

    Techniques: Cell Culture

    Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+  cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+  supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+  supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP,  β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.

    Journal: Scientific Reports

    Article Title: Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation

    doi: 10.1038/s41598-018-20241-3

    Figure Lengend Snippet: Crystal or particle clusters formed in BGP cell culture supernatant are morphologically and chemically distinct. Morphology of crystal clusters formed in BGP and in BGP supplemented with Mg 2+ cell culture supernatants, as overview (upper) and focused (lower) for EDX analysis ( a ). The averaged EDX-spectrum and resulting quantification of the crystal cluster isolated from Mg 2+ supplemented culture supernatant reveals reduced Ca (green) and P (purple), as visualized in the elemental map ( c ) compared to crystal clusters found in BGP treated cultures ( b ). In addition, Mg 2+ supplemented crystal clusters showed increased Na (blue) and Cl (orange) compared to BGP crystal clusters. Data are presented as mean of the analyses of ten individual crystal clusters. BGP, β -glycerophosphate; CPS, counts per second; EDX, energy dispersive spectroscopy; (k)eV, (kilo)-electron volt.

    Article Snippet: For calcification, medium was supplemented with 5% (v/v) FBS and 10 mM β -glycerophosphate (BGP, Merck Millipore, Massachusetts, USA).

    Techniques: Cell Culture, Isolation, Spectroscopy

    Impact of Mg 2+  on BGP-induced Ca 2+ -apatite formation. X-ray powder diffraction analysis of cell culture supernatants show presence of NaCl (*) and Ca-apatite (arrows) in BGP treated cultures (black line), but not in BGP cultures supplemented with Mg 2+  (gray line). Of note: as the BGP culture supplemented with Mg 2+  contained less precipitates; one measurement represents 21 cultures (6-well format) compared to 7 in the BGP condition in order to reach the detection threshold. BGP,  β -glycerophosphate.

    Journal: Scientific Reports

    Article Title: Magnesium prevents vascular calcification in vitro by inhibition of hydroxyapatite crystal formation

    doi: 10.1038/s41598-018-20241-3

    Figure Lengend Snippet: Impact of Mg 2+ on BGP-induced Ca 2+ -apatite formation. X-ray powder diffraction analysis of cell culture supernatants show presence of NaCl (*) and Ca-apatite (arrows) in BGP treated cultures (black line), but not in BGP cultures supplemented with Mg 2+ (gray line). Of note: as the BGP culture supplemented with Mg 2+ contained less precipitates; one measurement represents 21 cultures (6-well format) compared to 7 in the BGP condition in order to reach the detection threshold. BGP, β -glycerophosphate.

    Article Snippet: For calcification, medium was supplemented with 5% (v/v) FBS and 10 mM β -glycerophosphate (BGP, Merck Millipore, Massachusetts, USA).

    Techniques: Cell Culture