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Boster Bio β glycerophosphate
The influence of TNFα expressed by BV2 cells for VSMC calcification. a - f Alizarin red staining analysis of VSMC calcification in differently stimulated condition (40×), a group1 added 10% BV2 culture supernatant and 10 mM <t>β-glycerophosphate,</t> b group3 added 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα, c group5 10 mM β-glycerophosphate, d group2 added 10% BV2 culture supernatant, which was incubated with rabbit anti-mouse TNFα antibody, and 10 mM β-glycerophosphate, e group4 added 10 ng/ml mouse TNFα, f group6 added equal volume PBS. g-i qRT-PCR analysis for the expression of Runx2 and α-SMA in VSMC, g the mRNA expression of Runx2 and α-SMA in groups 1 and 2, h the mRNA expression of Runx2 and α-SMA in groups 3 and 4, i the mRNA expression of Runx2 and α-SMA in groups 5 and 6. Bars represent mean ± S.D. ( n ≥ 3). Significant difference marked with one ( p
β Glycerophosphate, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment"

Article Title: miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment

Journal: BMC Immunology

doi: 10.1186/s12865-019-0324-x

The influence of TNFα expressed by BV2 cells for VSMC calcification. a - f Alizarin red staining analysis of VSMC calcification in differently stimulated condition (40×), a group1 added 10% BV2 culture supernatant and 10 mM β-glycerophosphate, b group3 added 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα, c group5 10 mM β-glycerophosphate, d group2 added 10% BV2 culture supernatant, which was incubated with rabbit anti-mouse TNFα antibody, and 10 mM β-glycerophosphate, e group4 added 10 ng/ml mouse TNFα, f group6 added equal volume PBS. g-i qRT-PCR analysis for the expression of Runx2 and α-SMA in VSMC, g the mRNA expression of Runx2 and α-SMA in groups 1 and 2, h the mRNA expression of Runx2 and α-SMA in groups 3 and 4, i the mRNA expression of Runx2 and α-SMA in groups 5 and 6. Bars represent mean ± S.D. ( n ≥ 3). Significant difference marked with one ( p
Figure Legend Snippet: The influence of TNFα expressed by BV2 cells for VSMC calcification. a - f Alizarin red staining analysis of VSMC calcification in differently stimulated condition (40×), a group1 added 10% BV2 culture supernatant and 10 mM β-glycerophosphate, b group3 added 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα, c group5 10 mM β-glycerophosphate, d group2 added 10% BV2 culture supernatant, which was incubated with rabbit anti-mouse TNFα antibody, and 10 mM β-glycerophosphate, e group4 added 10 ng/ml mouse TNFα, f group6 added equal volume PBS. g-i qRT-PCR analysis for the expression of Runx2 and α-SMA in VSMC, g the mRNA expression of Runx2 and α-SMA in groups 1 and 2, h the mRNA expression of Runx2 and α-SMA in groups 3 and 4, i the mRNA expression of Runx2 and α-SMA in groups 5 and 6. Bars represent mean ± S.D. ( n ≥ 3). Significant difference marked with one ( p

Techniques Used: Staining, Incubation, Quantitative RT-PCR, Expressing

2) Product Images from "miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment"

Article Title: miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment

Journal: BMC Immunology

doi: 10.1186/s12865-019-0324-x

The influence of TNFα expressed by BV2 cells for VSMC calcification. a - f Alizarin red staining analysis of VSMC calcification in differently stimulated condition (40×), a group1 added 10% BV2 culture supernatant and 10 mM β-glycerophosphate, b group3 added 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα, c group5 10 mM β-glycerophosphate, d group2 added 10% BV2 culture supernatant, which was incubated with rabbit anti-mouse TNFα antibody, and 10 mM β-glycerophosphate, e group4 added 10 ng/ml mouse TNFα, f group6 added equal volume PBS. g-i qRT-PCR analysis for the expression of Runx2 and α-SMA in VSMC, g the mRNA expression of Runx2 and α-SMA in groups 1 and 2, h the mRNA expression of Runx2 and α-SMA in groups 3 and 4, i the mRNA expression of Runx2 and α-SMA in groups 5 and 6. Bars represent mean ± S.D. ( n ≥ 3). Significant difference marked with one ( p
Figure Legend Snippet: The influence of TNFα expressed by BV2 cells for VSMC calcification. a - f Alizarin red staining analysis of VSMC calcification in differently stimulated condition (40×), a group1 added 10% BV2 culture supernatant and 10 mM β-glycerophosphate, b group3 added 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα, c group5 10 mM β-glycerophosphate, d group2 added 10% BV2 culture supernatant, which was incubated with rabbit anti-mouse TNFα antibody, and 10 mM β-glycerophosphate, e group4 added 10 ng/ml mouse TNFα, f group6 added equal volume PBS. g-i qRT-PCR analysis for the expression of Runx2 and α-SMA in VSMC, g the mRNA expression of Runx2 and α-SMA in groups 1 and 2, h the mRNA expression of Runx2 and α-SMA in groups 3 and 4, i the mRNA expression of Runx2 and α-SMA in groups 5 and 6. Bars represent mean ± S.D. ( n ≥ 3). Significant difference marked with one ( p

Techniques Used: Staining, Incubation, Quantitative RT-PCR, Expressing

3) Product Images from "miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment"

Article Title: miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment

Journal: BMC Immunology

doi: 10.1186/s12865-019-0324-x

The influence of TNFα expressed by BV2 cells for VSMC calcification. a - f Alizarin red staining analysis of VSMC calcification in differently stimulated condition (40×), a group1 added 10% BV2 culture supernatant and 10 mM β-glycerophosphate, b group3 added 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα, c group5 10 mM β-glycerophosphate, d group2 added 10% BV2 culture supernatant, which was incubated with rabbit anti-mouse TNFα antibody, and 10 mM β-glycerophosphate, e group4 added 10 ng/ml mouse TNFα, f group6 added equal volume PBS. g-i qRT-PCR analysis for the expression of Runx2 and α-SMA in VSMC, g the mRNA expression of Runx2 and α-SMA in groups 1 and 2, h the mRNA expression of Runx2 and α-SMA in groups 3 and 4, i the mRNA expression of Runx2 and α-SMA in groups 5 and 6. Bars represent mean ± S.D. ( n ≥ 3). Significant difference marked with one ( p
Figure Legend Snippet: The influence of TNFα expressed by BV2 cells for VSMC calcification. a - f Alizarin red staining analysis of VSMC calcification in differently stimulated condition (40×), a group1 added 10% BV2 culture supernatant and 10 mM β-glycerophosphate, b group3 added 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα, c group5 10 mM β-glycerophosphate, d group2 added 10% BV2 culture supernatant, which was incubated with rabbit anti-mouse TNFα antibody, and 10 mM β-glycerophosphate, e group4 added 10 ng/ml mouse TNFα, f group6 added equal volume PBS. g-i qRT-PCR analysis for the expression of Runx2 and α-SMA in VSMC, g the mRNA expression of Runx2 and α-SMA in groups 1 and 2, h the mRNA expression of Runx2 and α-SMA in groups 3 and 4, i the mRNA expression of Runx2 and α-SMA in groups 5 and 6. Bars represent mean ± S.D. ( n ≥ 3). Significant difference marked with one ( p

Techniques Used: Staining, Incubation, Quantitative RT-PCR, Expressing

Related Articles

Modification:

Article Title: miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment
Article Snippet: .. Induction of VSMC calcification According to the report by Ma et al [ ] with some modification, the VSMCs were divided into 6 groups, cultured in 12-well plates with 1 ml DMEM with 10% FBS and 100 U/ml penicillin-streptomycin, and treated as follows: group 1 received 10% BV2 culture supernatant (treated with LPS) and 10 mM β-glycerophosphate (Sigma, Poole, Dorset, UK); group 2 received 10% BV2 culture supernatant (treated with LPS), which was incubated with 7 μl rabbit anti-mouse TNFα antibody (BOSTER Biological Technology Co. Ltd., China) at 4 °C for 12 h and 10 mM β-glycerophosphate; group 3 received 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα (BOSTER Biological Technology Co. ltd., China); group 4 received 10 ng/ml mouse TNFα; group 5 received 10 mM β-glycerophosphate; and group 6 received equal volume of PBS. .. The VSMCs of all groups were cultured at 37 °C and 5% CO2 for 5 days, and the culture supernatants were replaced at day 3.

Incubation:

Article Title: miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment
Article Snippet: .. Induction of VSMC calcification According to the report by Ma et al [ ] with some modification, the VSMCs were divided into 6 groups, cultured in 12-well plates with 1 ml DMEM with 10% FBS and 100 U/ml penicillin-streptomycin, and treated as follows: group 1 received 10% BV2 culture supernatant (treated with LPS) and 10 mM β-glycerophosphate (Sigma, Poole, Dorset, UK); group 2 received 10% BV2 culture supernatant (treated with LPS), which was incubated with 7 μl rabbit anti-mouse TNFα antibody (BOSTER Biological Technology Co. Ltd., China) at 4 °C for 12 h and 10 mM β-glycerophosphate; group 3 received 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα (BOSTER Biological Technology Co. ltd., China); group 4 received 10 ng/ml mouse TNFα; group 5 received 10 mM β-glycerophosphate; and group 6 received equal volume of PBS. .. The VSMCs of all groups were cultured at 37 °C and 5% CO2 for 5 days, and the culture supernatants were replaced at day 3.

Cell Culture:

Article Title: miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment
Article Snippet: .. Induction of VSMC calcification According to the report by Ma et al [ ] with some modification, the VSMCs were divided into 6 groups, cultured in 12-well plates with 1 ml DMEM with 10% FBS and 100 U/ml penicillin-streptomycin, and treated as follows: group 1 received 10% BV2 culture supernatant (treated with LPS) and 10 mM β-glycerophosphate (Sigma, Poole, Dorset, UK); group 2 received 10% BV2 culture supernatant (treated with LPS), which was incubated with 7 μl rabbit anti-mouse TNFα antibody (BOSTER Biological Technology Co. Ltd., China) at 4 °C for 12 h and 10 mM β-glycerophosphate; group 3 received 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα (BOSTER Biological Technology Co. ltd., China); group 4 received 10 ng/ml mouse TNFα; group 5 received 10 mM β-glycerophosphate; and group 6 received equal volume of PBS. .. The VSMCs of all groups were cultured at 37 °C and 5% CO2 for 5 days, and the culture supernatants were replaced at day 3.

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    Boster Bio β glycerophosphate
    The influence of TNFα expressed by BV2 cells for VSMC calcification. a - f Alizarin red staining analysis of VSMC calcification in differently stimulated condition (40×), a group1 added 10% BV2 culture supernatant and 10 mM <t>β-glycerophosphate,</t> b group3 added 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα, c group5 10 mM β-glycerophosphate, d group2 added 10% BV2 culture supernatant, which was incubated with rabbit anti-mouse TNFα antibody, and 10 mM β-glycerophosphate, e group4 added 10 ng/ml mouse TNFα, f group6 added equal volume PBS. g-i qRT-PCR analysis for the expression of Runx2 and α-SMA in VSMC, g the mRNA expression of Runx2 and α-SMA in groups 1 and 2, h the mRNA expression of Runx2 and α-SMA in groups 3 and 4, i the mRNA expression of Runx2 and α-SMA in groups 5 and 6. Bars represent mean ± S.D. ( n ≥ 3). Significant difference marked with one ( p
    β Glycerophosphate, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β glycerophosphate/product/Boster Bio
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    β glycerophosphate - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    88
    Boster Bio β sodium glycerophosphate
    The influence of TNFα expressed by BV2 cells for VSMC calcification. a - f Alizarin red staining analysis of VSMC calcification in differently stimulated condition (40×), a group1 added 10% BV2 culture supernatant and 10 mM <t>β-glycerophosphate,</t> b group3 added 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα, c group5 10 mM β-glycerophosphate, d group2 added 10% BV2 culture supernatant, which was incubated with rabbit anti-mouse TNFα antibody, and 10 mM β-glycerophosphate, e group4 added 10 ng/ml mouse TNFα, f group6 added equal volume PBS. g-i qRT-PCR analysis for the expression of Runx2 and α-SMA in VSMC, g the mRNA expression of Runx2 and α-SMA in groups 1 and 2, h the mRNA expression of Runx2 and α-SMA in groups 3 and 4, i the mRNA expression of Runx2 and α-SMA in groups 5 and 6. Bars represent mean ± S.D. ( n ≥ 3). Significant difference marked with one ( p
    β Sodium Glycerophosphate, supplied by Boster Bio, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β sodium glycerophosphate/product/Boster Bio
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    β sodium glycerophosphate - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

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    The influence of TNFα expressed by BV2 cells for VSMC calcification. a - f Alizarin red staining analysis of VSMC calcification in differently stimulated condition (40×), a group1 added 10% BV2 culture supernatant and 10 mM β-glycerophosphate, b group3 added 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα, c group5 10 mM β-glycerophosphate, d group2 added 10% BV2 culture supernatant, which was incubated with rabbit anti-mouse TNFα antibody, and 10 mM β-glycerophosphate, e group4 added 10 ng/ml mouse TNFα, f group6 added equal volume PBS. g-i qRT-PCR analysis for the expression of Runx2 and α-SMA in VSMC, g the mRNA expression of Runx2 and α-SMA in groups 1 and 2, h the mRNA expression of Runx2 and α-SMA in groups 3 and 4, i the mRNA expression of Runx2 and α-SMA in groups 5 and 6. Bars represent mean ± S.D. ( n ≥ 3). Significant difference marked with one ( p

    Journal: BMC Immunology

    Article Title: miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment

    doi: 10.1186/s12865-019-0324-x

    Figure Lengend Snippet: The influence of TNFα expressed by BV2 cells for VSMC calcification. a - f Alizarin red staining analysis of VSMC calcification in differently stimulated condition (40×), a group1 added 10% BV2 culture supernatant and 10 mM β-glycerophosphate, b group3 added 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα, c group5 10 mM β-glycerophosphate, d group2 added 10% BV2 culture supernatant, which was incubated with rabbit anti-mouse TNFα antibody, and 10 mM β-glycerophosphate, e group4 added 10 ng/ml mouse TNFα, f group6 added equal volume PBS. g-i qRT-PCR analysis for the expression of Runx2 and α-SMA in VSMC, g the mRNA expression of Runx2 and α-SMA in groups 1 and 2, h the mRNA expression of Runx2 and α-SMA in groups 3 and 4, i the mRNA expression of Runx2 and α-SMA in groups 5 and 6. Bars represent mean ± S.D. ( n ≥ 3). Significant difference marked with one ( p

    Article Snippet: Induction of VSMC calcification According to the report by Ma et al [ ] with some modification, the VSMCs were divided into 6 groups, cultured in 12-well plates with 1 ml DMEM with 10% FBS and 100 U/ml penicillin-streptomycin, and treated as follows: group 1 received 10% BV2 culture supernatant (treated with LPS) and 10 mM β-glycerophosphate (Sigma, Poole, Dorset, UK); group 2 received 10% BV2 culture supernatant (treated with LPS), which was incubated with 7 μl rabbit anti-mouse TNFα antibody (BOSTER Biological Technology Co. Ltd., China) at 4 °C for 12 h and 10 mM β-glycerophosphate; group 3 received 10 mM β-glycerophosphate and 10 ng/ml mouse TNFα (BOSTER Biological Technology Co. ltd., China); group 4 received 10 ng/ml mouse TNFα; group 5 received 10 mM β-glycerophosphate; and group 6 received equal volume of PBS.

    Techniques: Staining, Incubation, Quantitative RT-PCR, Expressing