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Avantor β glycerophosphate
β Glycerophosphate, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β glycerophosphate/product/Avantor
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
β glycerophosphate - by Bioz Stars, 2020-09
92/100 stars

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Western Blot:

Article Title: Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages
Article Snippet: .. Western Blotting After removing the medium, cells were washed in PBS, scraped, and lysed in 30 μ L of lysis buffer (10 mM Tris (Merck, Billerica, MA, USA), 100 mM NaCl (Merck, Billerica, MA, USA), 10% glycerol (Merck, Billerica, MA, USA), 1% Nonidet P-40 Substitute (Sigma-Aldrich, St. Louis, MO, USA), 0,1% sodium dodecyl sulfate (MP Biochemicals, Solon, OH, USA), 0,5% sodium deoxycholate (Merck, Billerica, MA, USA)) containing a proteinase inhibitor “Complete” cocktail (Roche Molecular Biochemicals, Basel, Switzerland) and phosphatase inhibitors (25 mM Na3 VO4 , 250 mM 4-nitrophenyl phosphate (Sigma-Aldrich, St. Louis, MO, USA), 250 mM β -glycerophosphate (VWR, Radnor, PA, USA), and 125 mM NaF (Merck, Billerica, MA, USA) at a 1/25 dilution in H2 O) for 30 minutes at 4°C. .. Lysates were then centrifuged at 15 700 rcf for 10 minutes (Eppendorf Microcentrifuge 5415R).

Article Title: Characterization of the role of TMEM45A in cancer cell sensitivity to cisplatin
Article Snippet: .. Western blot After the incubation, cells were lysed in lysis buffer solution containing Trizma 40 mM (TRIS base) (Sigma-Aldrich), KCL 150 mM (Merck Millipore) and Tritriplex III 1 mM (Merck Millipore) with MilliQ water, Triton X100 1% (Sigma-Aldrich), phosphatase inhibitor cocktail (PIC, Roche), and phosphatase inhibitor buffer (PIB containing sodium orthovanadate (Na3 VO3 ) (Sigma-Aldrich), 4-nitrophenylphosphate (PNPP) (Sigma-Aldrich), β-glycerophosphate (VWR) and sodium fluoride (NaF) (Merck)). .. Then 15 μg of proteins were mixed with MilliQ water and blue sample buffer composed of DTT (dithiothreitol) 5% and LDS (lithium dodecyl sulfate) sample buffer 4 × (Thermo Fisher Buffer) and heated up during 10 min to 70 °C.

Incubation:

Article Title: Characterization of the role of TMEM45A in cancer cell sensitivity to cisplatin
Article Snippet: .. Western blot After the incubation, cells were lysed in lysis buffer solution containing Trizma 40 mM (TRIS base) (Sigma-Aldrich), KCL 150 mM (Merck Millipore) and Tritriplex III 1 mM (Merck Millipore) with MilliQ water, Triton X100 1% (Sigma-Aldrich), phosphatase inhibitor cocktail (PIC, Roche), and phosphatase inhibitor buffer (PIB containing sodium orthovanadate (Na3 VO3 ) (Sigma-Aldrich), 4-nitrophenylphosphate (PNPP) (Sigma-Aldrich), β-glycerophosphate (VWR) and sodium fluoride (NaF) (Merck)). .. Then 15 μg of proteins were mixed with MilliQ water and blue sample buffer composed of DTT (dithiothreitol) 5% and LDS (lithium dodecyl sulfate) sample buffer 4 × (Thermo Fisher Buffer) and heated up during 10 min to 70 °C.

Lysis:

Article Title: Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages
Article Snippet: .. Western Blotting After removing the medium, cells were washed in PBS, scraped, and lysed in 30 μ L of lysis buffer (10 mM Tris (Merck, Billerica, MA, USA), 100 mM NaCl (Merck, Billerica, MA, USA), 10% glycerol (Merck, Billerica, MA, USA), 1% Nonidet P-40 Substitute (Sigma-Aldrich, St. Louis, MO, USA), 0,1% sodium dodecyl sulfate (MP Biochemicals, Solon, OH, USA), 0,5% sodium deoxycholate (Merck, Billerica, MA, USA)) containing a proteinase inhibitor “Complete” cocktail (Roche Molecular Biochemicals, Basel, Switzerland) and phosphatase inhibitors (25 mM Na3 VO4 , 250 mM 4-nitrophenyl phosphate (Sigma-Aldrich, St. Louis, MO, USA), 250 mM β -glycerophosphate (VWR, Radnor, PA, USA), and 125 mM NaF (Merck, Billerica, MA, USA) at a 1/25 dilution in H2 O) for 30 minutes at 4°C. .. Lysates were then centrifuged at 15 700 rcf for 10 minutes (Eppendorf Microcentrifuge 5415R).

Article Title: Characterization of the role of TMEM45A in cancer cell sensitivity to cisplatin
Article Snippet: .. Western blot After the incubation, cells were lysed in lysis buffer solution containing Trizma 40 mM (TRIS base) (Sigma-Aldrich), KCL 150 mM (Merck Millipore) and Tritriplex III 1 mM (Merck Millipore) with MilliQ water, Triton X100 1% (Sigma-Aldrich), phosphatase inhibitor cocktail (PIC, Roche), and phosphatase inhibitor buffer (PIB containing sodium orthovanadate (Na3 VO3 ) (Sigma-Aldrich), 4-nitrophenylphosphate (PNPP) (Sigma-Aldrich), β-glycerophosphate (VWR) and sodium fluoride (NaF) (Merck)). .. Then 15 μg of proteins were mixed with MilliQ water and blue sample buffer composed of DTT (dithiothreitol) 5% and LDS (lithium dodecyl sulfate) sample buffer 4 × (Thermo Fisher Buffer) and heated up during 10 min to 70 °C.

Protease Inhibitor:

Article Title: Insulin-Sensitizing Therapy Attenuates Type 2 Diabetes-Mediated Mammary Tumor Progression
Article Snippet: .. Tissues were lysed in buffer (pH 7.4) containing 50 mmol/l Tris, 150 mmol/l NaCl, 1 mmol/l EDTA, 1.25% CHAPS (Roche Applied Science), 1 mmol/l sodium orthovanadate, 2 mmol/l sodium fluoride, 10 mmol/l sodium pyrophosphate (Sigma Aldrich), 8 mmol/l β-glycerophosphate (VWR, West Chester, PA), and Complete Protease Inhibitor Cocktail (Roche). .. After denaturation, the proteins were subjected to SDS-PAGE (8% Tris-glycine gel; Invitrogen, Carlsbad, CA) and transferred to a nitrocellulose membrane (Bio-Rad, Hercules, CA).

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    Avantor pngase f
    Recovery of glycans compared with the 6-GU standard. The total abundance of the 5 analyzed glycans in a single run were combined and divided by the abundance of the internal standard for that run. All results of the <t>PNGase</t> F replicates were then averaged (blue), and all of the results for the NaOCl replicates were averaged (red).
    Pngase F, supplied by Avantor, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pngase f/product/Avantor
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pngase f - by Bioz Stars, 2020-09
    93/100 stars
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    Recovery of glycans compared with the 6-GU standard. The total abundance of the 5 analyzed glycans in a single run were combined and divided by the abundance of the internal standard for that run. All results of the PNGase F replicates were then averaged (blue), and all of the results for the NaOCl replicates were averaged (red).

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols

    doi: 10.7171/jbt.19-3004-001

    Figure Lengend Snippet: Recovery of glycans compared with the 6-GU standard. The total abundance of the 5 analyzed glycans in a single run were combined and divided by the abundance of the internal standard for that run. All results of the PNGase F replicates were then averaged (blue), and all of the results for the NaOCl replicates were averaged (red).

    Article Snippet: The PNGase F samples were then cleaned using a C18 extraction column (Avantor Performance Materials, Radnor, PA, USA) and dried down.

    Techniques:

    SRM LC traces of the ProA-labeled 6-GU standard and the ProA-labeled N -linked glycans from fetuin released using the PNGase F and NaOCl protocols. LC traces of the 5 most abundant glycans of fetuin [A2G2S1 (dark blue), A2G2S2 (red), A3G3S2 (green), A3G3S3 (gray), and A3G3S4 (light blue)] from SRM analysis following the PNGase F release (top) and NaOCl release (bottom) protocols.

    Journal: Journal of Biomolecular Techniques : JBT

    Article Title: N-linked Glycan Release Efficiency: A Quantitative Comparison between NaOCl and PNGase F Release Protocols

    doi: 10.7171/jbt.19-3004-001

    Figure Lengend Snippet: SRM LC traces of the ProA-labeled 6-GU standard and the ProA-labeled N -linked glycans from fetuin released using the PNGase F and NaOCl protocols. LC traces of the 5 most abundant glycans of fetuin [A2G2S1 (dark blue), A2G2S2 (red), A3G3S2 (green), A3G3S3 (gray), and A3G3S4 (light blue)] from SRM analysis following the PNGase F release (top) and NaOCl release (bottom) protocols.

    Article Snippet: The PNGase F samples were then cleaned using a C18 extraction column (Avantor Performance Materials, Radnor, PA, USA) and dried down.

    Techniques: Labeling