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Promega β globin utr luc rna
Inhibition of <t>RNA</t> translation. (A) Transient replicon assay. A luciferase-reporting replicon (10 μg) was electroporated into BHK-21 cells. The transfected cells were immediately incubated with 0.9% DMSO or NITD-451 at the indicated concentrations. Luciferase activities were measured at 2, 4, 6, 16, 24, and 30 h posttransfection. (B) In vitro translation inhibition assay. A luciferase reporter replicon RNA of DENV-1 (strain Western Pacific) or reporter RNAs <t>(DENV-2–UTR-Luc</t> and <t>β-globin</t> UTR-Luc) were incubated with the human cell lysates (from the Pierce human in vitro protein expression kit) in the presence of NITD-451, NITD-452, cycloheximide, or DMSO (0.45%). After incubation at 30°C for 3.5 h, luciferase activity was measured by a luciferase assay system (Promega). Average results and standard deviations ( n = 3) are presented for all experiments.
β Globin Utr Luc Rna, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β globin utr luc rna/product/Promega
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
β globin utr luc rna - by Bioz Stars, 2020-07
85/100 stars

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1) Product Images from "A Translation Inhibitor That Suppresses Dengue Virus In Vitro and In Vivo ▿ ▿ †"

Article Title: A Translation Inhibitor That Suppresses Dengue Virus In Vitro and In Vivo ▿ ▿ †

Journal: Antimicrobial Agents and Chemotherapy

doi: 10.1128/AAC.00620-11

Inhibition of RNA translation. (A) Transient replicon assay. A luciferase-reporting replicon (10 μg) was electroporated into BHK-21 cells. The transfected cells were immediately incubated with 0.9% DMSO or NITD-451 at the indicated concentrations. Luciferase activities were measured at 2, 4, 6, 16, 24, and 30 h posttransfection. (B) In vitro translation inhibition assay. A luciferase reporter replicon RNA of DENV-1 (strain Western Pacific) or reporter RNAs (DENV-2–UTR-Luc and β-globin UTR-Luc) were incubated with the human cell lysates (from the Pierce human in vitro protein expression kit) in the presence of NITD-451, NITD-452, cycloheximide, or DMSO (0.45%). After incubation at 30°C for 3.5 h, luciferase activity was measured by a luciferase assay system (Promega). Average results and standard deviations ( n = 3) are presented for all experiments.
Figure Legend Snippet: Inhibition of RNA translation. (A) Transient replicon assay. A luciferase-reporting replicon (10 μg) was electroporated into BHK-21 cells. The transfected cells were immediately incubated with 0.9% DMSO or NITD-451 at the indicated concentrations. Luciferase activities were measured at 2, 4, 6, 16, 24, and 30 h posttransfection. (B) In vitro translation inhibition assay. A luciferase reporter replicon RNA of DENV-1 (strain Western Pacific) or reporter RNAs (DENV-2–UTR-Luc and β-globin UTR-Luc) were incubated with the human cell lysates (from the Pierce human in vitro protein expression kit) in the presence of NITD-451, NITD-452, cycloheximide, or DMSO (0.45%). After incubation at 30°C for 3.5 h, luciferase activity was measured by a luciferase assay system (Promega). Average results and standard deviations ( n = 3) are presented for all experiments.

Techniques Used: Inhibition, Luciferase, Transfection, Incubation, In Vitro, Western Blot, Expressing, Activity Assay

Related Articles

Polymerase Chain Reaction:

Article Title: A Translation Inhibitor That Suppresses Dengue Virus In Vitro and In Vivo ▿ ▿ †
Article Snippet: .. The cDNA plasmids for DENV-2–UTR-Luc RNA and β-globin UTR-Luc RNA were constructed by using overlapping PCR followed by cloning the PCR products into the plasmid vector pGL4.7 (Promega) at the NheI and BamHI sites and at the NheI and NruI sites, respectively. .. In vitro translation was performed following the manufacturer's protocol with modifications.

Clone Assay:

Article Title: A Translation Inhibitor That Suppresses Dengue Virus In Vitro and In Vivo ▿ ▿ †
Article Snippet: .. The cDNA plasmids for DENV-2–UTR-Luc RNA and β-globin UTR-Luc RNA were constructed by using overlapping PCR followed by cloning the PCR products into the plasmid vector pGL4.7 (Promega) at the NheI and BamHI sites and at the NheI and NruI sites, respectively. .. In vitro translation was performed following the manufacturer's protocol with modifications.

Construct:

Article Title: A Translation Inhibitor That Suppresses Dengue Virus In Vitro and In Vivo ▿ ▿ †
Article Snippet: .. The cDNA plasmids for DENV-2–UTR-Luc RNA and β-globin UTR-Luc RNA were constructed by using overlapping PCR followed by cloning the PCR products into the plasmid vector pGL4.7 (Promega) at the NheI and BamHI sites and at the NheI and NruI sites, respectively. .. In vitro translation was performed following the manufacturer's protocol with modifications.

Plasmid Preparation:

Article Title: A Translation Inhibitor That Suppresses Dengue Virus In Vitro and In Vivo ▿ ▿ †
Article Snippet: .. The cDNA plasmids for DENV-2–UTR-Luc RNA and β-globin UTR-Luc RNA were constructed by using overlapping PCR followed by cloning the PCR products into the plasmid vector pGL4.7 (Promega) at the NheI and BamHI sites and at the NheI and NruI sites, respectively. .. In vitro translation was performed following the manufacturer's protocol with modifications.

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    Promega β globin utr luc rna
    Inhibition of <t>RNA</t> translation. (A) Transient replicon assay. A luciferase-reporting replicon (10 μg) was electroporated into BHK-21 cells. The transfected cells were immediately incubated with 0.9% DMSO or NITD-451 at the indicated concentrations. Luciferase activities were measured at 2, 4, 6, 16, 24, and 30 h posttransfection. (B) In vitro translation inhibition assay. A luciferase reporter replicon RNA of DENV-1 (strain Western Pacific) or reporter RNAs <t>(DENV-2–UTR-Luc</t> and <t>β-globin</t> UTR-Luc) were incubated with the human cell lysates (from the Pierce human in vitro protein expression kit) in the presence of NITD-451, NITD-452, cycloheximide, or DMSO (0.45%). After incubation at 30°C for 3.5 h, luciferase activity was measured by a luciferase assay system (Promega). Average results and standard deviations ( n = 3) are presented for all experiments.
    β Globin Utr Luc Rna, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β globin utr luc rna/product/Promega
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    β globin utr luc rna - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

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    Inhibition of RNA translation. (A) Transient replicon assay. A luciferase-reporting replicon (10 μg) was electroporated into BHK-21 cells. The transfected cells were immediately incubated with 0.9% DMSO or NITD-451 at the indicated concentrations. Luciferase activities were measured at 2, 4, 6, 16, 24, and 30 h posttransfection. (B) In vitro translation inhibition assay. A luciferase reporter replicon RNA of DENV-1 (strain Western Pacific) or reporter RNAs (DENV-2–UTR-Luc and β-globin UTR-Luc) were incubated with the human cell lysates (from the Pierce human in vitro protein expression kit) in the presence of NITD-451, NITD-452, cycloheximide, or DMSO (0.45%). After incubation at 30°C for 3.5 h, luciferase activity was measured by a luciferase assay system (Promega). Average results and standard deviations ( n = 3) are presented for all experiments.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: A Translation Inhibitor That Suppresses Dengue Virus In Vitro and In Vivo ▿ ▿ †

    doi: 10.1128/AAC.00620-11

    Figure Lengend Snippet: Inhibition of RNA translation. (A) Transient replicon assay. A luciferase-reporting replicon (10 μg) was electroporated into BHK-21 cells. The transfected cells were immediately incubated with 0.9% DMSO or NITD-451 at the indicated concentrations. Luciferase activities were measured at 2, 4, 6, 16, 24, and 30 h posttransfection. (B) In vitro translation inhibition assay. A luciferase reporter replicon RNA of DENV-1 (strain Western Pacific) or reporter RNAs (DENV-2–UTR-Luc and β-globin UTR-Luc) were incubated with the human cell lysates (from the Pierce human in vitro protein expression kit) in the presence of NITD-451, NITD-452, cycloheximide, or DMSO (0.45%). After incubation at 30°C for 3.5 h, luciferase activity was measured by a luciferase assay system (Promega). Average results and standard deviations ( n = 3) are presented for all experiments.

    Article Snippet: The cDNA plasmids for DENV-2–UTR-Luc RNA and β-globin UTR-Luc RNA were constructed by using overlapping PCR followed by cloning the PCR products into the plasmid vector pGL4.7 (Promega) at the NheI and BamHI sites and at the NheI and NruI sites, respectively.

    Techniques: Inhibition, Luciferase, Transfection, Incubation, In Vitro, Western Blot, Expressing, Activity Assay