are luc activity  (Millipore)


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    Name:
    Luciferase
    Description:
    Luciferase is a class of enzymes naturally found in organisms like protists fungi insects bacteria which belong to phylogenic kingdom It is used as a molecular marker for biological imaging as it specifically cleaves luciferin substrate which results in emitting light
    Catalog Number:
    10411523001
    Price:
    None
    Applications:
    Determination of ATP in ATP-generating or ATP-consuming reactions. Determination of the activity of enzymes such as creatine kinase and ATPases. Under optimal conditions, 1 photon is released per molecule ATP in the bioluminescence assay.One mg is sufficient for approximately 5,000 ATP determinations in the concentration range of 0.1 nM to 1 muM.
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    Structured Review

    Millipore are luc activity
    Phf2 and ChREBP decrease oxidative stress through Nrf2 activation. Mice, injected with either GFP or Phf2 overexpressing adenovirus, were studied 3 weeks later in fed state. a Representative Western blot analysis of Nrf2 protein content and Nrf2-regulated genes ( n = 10 mice per group). b – d Nrf2 was inhibited in cultured hepatocytes overexpressing Phf2. b Western blot analysis of proteins involved in oxidative stress defenses ( n = 3). GSH and NADPH contents ( c ) in addition to ROS levels and hepatocyte apoptosis ( d ) were determined after incubation with 480 μM palmitate for 24 h ( n = 3). e UCSC genome browser image illustrating normalized tag counts for Phf2, ChREBP, H3K9me2, H3K4me3, and RNA polII at the Nrf2 promoter. ( f , g ) Phf2 was inhibited in cultured hepatocytes. ChIP for H3K9me2, ChREBP, and RNA polII, in addition to chromatin accessibility at the Nrf2 promoter and Nrf2 activity on the <t>ARE-luc</t> construct shown ( n = 3). h – j ChREBP expression was inhibited in cultured hepatocytes overexpressing Phf2. h Representative western blot analysis showing the contribution of ChREBP to the regulation of Nrf2 and Nrf2-regulated gene expression. i Nrf2 activity on the ARE-luc construct shown. j Relative ROS levels and measurement of hepatocyte apoptosis were determined after incubation with 480 μM palmitate for 24 h ( n = 3). All error bars represent mean ± SEM. Statistical analyses were made using Anova, followed by Bonferonni’s test. * P
    Luciferase is a class of enzymes naturally found in organisms like protists fungi insects bacteria which belong to phylogenic kingdom It is used as a molecular marker for biological imaging as it specifically cleaves luciferin substrate which results in emitting light
    https://www.bioz.com/result/are luc activity/product/Millipore
    Average 99 stars, based on 291 article reviews
    Price from $9.99 to $1999.99
    are luc activity - by Bioz Stars, 2020-07
    99/100 stars

    Images

    1) Product Images from "The histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity"

    Article Title: The histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity

    Journal: Nature Communications

    doi: 10.1038/s41467-018-04361-y

    Phf2 and ChREBP decrease oxidative stress through Nrf2 activation. Mice, injected with either GFP or Phf2 overexpressing adenovirus, were studied 3 weeks later in fed state. a Representative Western blot analysis of Nrf2 protein content and Nrf2-regulated genes ( n = 10 mice per group). b – d Nrf2 was inhibited in cultured hepatocytes overexpressing Phf2. b Western blot analysis of proteins involved in oxidative stress defenses ( n = 3). GSH and NADPH contents ( c ) in addition to ROS levels and hepatocyte apoptosis ( d ) were determined after incubation with 480 μM palmitate for 24 h ( n = 3). e UCSC genome browser image illustrating normalized tag counts for Phf2, ChREBP, H3K9me2, H3K4me3, and RNA polII at the Nrf2 promoter. ( f , g ) Phf2 was inhibited in cultured hepatocytes. ChIP for H3K9me2, ChREBP, and RNA polII, in addition to chromatin accessibility at the Nrf2 promoter and Nrf2 activity on the ARE-luc construct shown ( n = 3). h – j ChREBP expression was inhibited in cultured hepatocytes overexpressing Phf2. h Representative western blot analysis showing the contribution of ChREBP to the regulation of Nrf2 and Nrf2-regulated gene expression. i Nrf2 activity on the ARE-luc construct shown. j Relative ROS levels and measurement of hepatocyte apoptosis were determined after incubation with 480 μM palmitate for 24 h ( n = 3). All error bars represent mean ± SEM. Statistical analyses were made using Anova, followed by Bonferonni’s test. * P
    Figure Legend Snippet: Phf2 and ChREBP decrease oxidative stress through Nrf2 activation. Mice, injected with either GFP or Phf2 overexpressing adenovirus, were studied 3 weeks later in fed state. a Representative Western blot analysis of Nrf2 protein content and Nrf2-regulated genes ( n = 10 mice per group). b – d Nrf2 was inhibited in cultured hepatocytes overexpressing Phf2. b Western blot analysis of proteins involved in oxidative stress defenses ( n = 3). GSH and NADPH contents ( c ) in addition to ROS levels and hepatocyte apoptosis ( d ) were determined after incubation with 480 μM palmitate for 24 h ( n = 3). e UCSC genome browser image illustrating normalized tag counts for Phf2, ChREBP, H3K9me2, H3K4me3, and RNA polII at the Nrf2 promoter. ( f , g ) Phf2 was inhibited in cultured hepatocytes. ChIP for H3K9me2, ChREBP, and RNA polII, in addition to chromatin accessibility at the Nrf2 promoter and Nrf2 activity on the ARE-luc construct shown ( n = 3). h – j ChREBP expression was inhibited in cultured hepatocytes overexpressing Phf2. h Representative western blot analysis showing the contribution of ChREBP to the regulation of Nrf2 and Nrf2-regulated gene expression. i Nrf2 activity on the ARE-luc construct shown. j Relative ROS levels and measurement of hepatocyte apoptosis were determined after incubation with 480 μM palmitate for 24 h ( n = 3). All error bars represent mean ± SEM. Statistical analyses were made using Anova, followed by Bonferonni’s test. * P

    Techniques Used: Activation Assay, Mouse Assay, Injection, Western Blot, Cell Culture, Incubation, Chromatin Immunoprecipitation, Activity Assay, Construct, Expressing

    2) Product Images from "Identification of receptor-type protein tyrosine phosphatase μ as a new marker for osteocytes"

    Article Title: Identification of receptor-type protein tyrosine phosphatase μ as a new marker for osteocytes

    Journal: Histochemistry and Cell Biology

    doi: 10.1007/s00418-015-1319-1

    β-Galactosidase activity in capillary outgrowth of metatarsals. Cultures of metatarsals of 17-day-old RPTPμ-knock-out/LacZ knock-in fetuses were stained for the endothelial cell marker PECAM (CD31) ( a ) and LacZ ( b ). The capillary outgrowth shows a clear blue staining, illustrating the expression of RPTPµ in these cells. Bars represent 200 µm
    Figure Legend Snippet: β-Galactosidase activity in capillary outgrowth of metatarsals. Cultures of metatarsals of 17-day-old RPTPμ-knock-out/LacZ knock-in fetuses were stained for the endothelial cell marker PECAM (CD31) ( a ) and LacZ ( b ). The capillary outgrowth shows a clear blue staining, illustrating the expression of RPTPµ in these cells. Bars represent 200 µm

    Techniques Used: Activity Assay, Knock-Out, Knock-In, Staining, Marker, Expressing

    Expression of RPTPµ in bone. Calvariae ( a ), metatarsals ( b ) and tibiae ( c ) of 5-day-old neonatal and tibiae ( d ) of adult RPTPμ-knock-out/LacZ knock-in mice were stained for β-galactosidase activity using X-gal. Within the calvariae, the cellular network of osteocytes is stained blue . In neonatal metatarsals and tibiae, the osteocytes ( arrows ) show a deep blue color , while the osteoblasts ( arrowheads ) are not stained. The adult tibiae also show that the blue staining representing RPTPµ expression is only present in osteocytes ( arrows ), while lining cells ( arrowheads ) are negative. The cellular processes ( cp ) are clearly visible. Bars : a 50 µm; b 100 µm; c , d 25 µm
    Figure Legend Snippet: Expression of RPTPµ in bone. Calvariae ( a ), metatarsals ( b ) and tibiae ( c ) of 5-day-old neonatal and tibiae ( d ) of adult RPTPμ-knock-out/LacZ knock-in mice were stained for β-galactosidase activity using X-gal. Within the calvariae, the cellular network of osteocytes is stained blue . In neonatal metatarsals and tibiae, the osteocytes ( arrows ) show a deep blue color , while the osteoblasts ( arrowheads ) are not stained. The adult tibiae also show that the blue staining representing RPTPµ expression is only present in osteocytes ( arrows ), while lining cells ( arrowheads ) are negative. The cellular processes ( cp ) are clearly visible. Bars : a 50 µm; b 100 µm; c , d 25 µm

    Techniques Used: Expressing, Knock-Out, Knock-In, Mouse Assay, Staining, Activity Assay

    BMPs stimulate, while Noggin inhibits the differentiation of osteocytes. Bone marrow stromal cells (MCS) of RPTPμ-knock-out/LacZ knock-in mice were cultured under osteogenic conditions for 21 days. Thereafter, the cell cultures were processed for β-galactosidase activity analysis by a LacZ enzymatic assay ( a ) or X-gal staining ( b , c ). Mineralization of the cultures was examined by alizarin red S staining ( b , c ). The enzymatic assay shows that BMP-6 clearly stimulates, while Noggin inhibits osteocyte formation. Staining of the cultures with X-gal confirms this observation. The wells stimulated with BMP-6 show more blue staining than the control wells; the wells cultured in the presence of Noggin show hardly any blue staining ( b ). The same observation can be made from the larger magnification of the wells ( c ). Alizarin red S staining is also stimulated by BMP-6 and reduced by Noggin, but to a lesser extend ( b , c ). Bars 200 µm; ** p
    Figure Legend Snippet: BMPs stimulate, while Noggin inhibits the differentiation of osteocytes. Bone marrow stromal cells (MCS) of RPTPμ-knock-out/LacZ knock-in mice were cultured under osteogenic conditions for 21 days. Thereafter, the cell cultures were processed for β-galactosidase activity analysis by a LacZ enzymatic assay ( a ) or X-gal staining ( b , c ). Mineralization of the cultures was examined by alizarin red S staining ( b , c ). The enzymatic assay shows that BMP-6 clearly stimulates, while Noggin inhibits osteocyte formation. Staining of the cultures with X-gal confirms this observation. The wells stimulated with BMP-6 show more blue staining than the control wells; the wells cultured in the presence of Noggin show hardly any blue staining ( b ). The same observation can be made from the larger magnification of the wells ( c ). Alizarin red S staining is also stimulated by BMP-6 and reduced by Noggin, but to a lesser extend ( b , c ). Bars 200 µm; ** p

    Techniques Used: Knock-Out, Knock-In, Mouse Assay, Cell Culture, Activity Assay, Enzymatic Assay, Staining

    Osteocytes can be generated in vitro. Bone marrow stromal cells (MSC) of RPTPμ-knock-out/LacZ knock-in mice were cultured for 21 days under osteogenic conditions and stained for β-galactosidase activity using X-gal. Within the cultures, blue -stained cells can be observed ( a ). The number of blue -stained cells is markedly increased when BMPs were added to the medium ( b ). Close observation shows that within the mineralized (M) nodules osteocytes are present, although the number of blue -stained cells is higher in the non-mineralized areas of the nodules. Histological analysis of the MSC cultures shows that only cells that are embedded within matrix show β-galactosidase activity ( c ). Some of these cells show cellular processes, which is very characteristic for osteocytes, which can be observed in a larger magnification of the same area ( arrowheads d ). Bars a , b 250 µm; c , d 25 µm
    Figure Legend Snippet: Osteocytes can be generated in vitro. Bone marrow stromal cells (MSC) of RPTPμ-knock-out/LacZ knock-in mice were cultured for 21 days under osteogenic conditions and stained for β-galactosidase activity using X-gal. Within the cultures, blue -stained cells can be observed ( a ). The number of blue -stained cells is markedly increased when BMPs were added to the medium ( b ). Close observation shows that within the mineralized (M) nodules osteocytes are present, although the number of blue -stained cells is higher in the non-mineralized areas of the nodules. Histological analysis of the MSC cultures shows that only cells that are embedded within matrix show β-galactosidase activity ( c ). Some of these cells show cellular processes, which is very characteristic for osteocytes, which can be observed in a larger magnification of the same area ( arrowheads d ). Bars a , b 250 µm; c , d 25 µm

    Techniques Used: Generated, In Vitro, Knock-Out, Knock-In, Mouse Assay, Cell Culture, Staining, Activity Assay

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    Article Snippet: .. HEK293T cells were transfected with the following lentiviral constructs with packaging plasmids, using polyethylenimine reagent: sh-luciferase (sh-Luc), sh-SIRT1, and sh-NLRP3 constructs (Sigma-Aldrich, St. Louis, MO, USA). .. Lentiviral supernatants were collected and filtered at 48 and 72 h after transfection.

    Article Title: Generation of clinical-grade CD19-specific CAR-modified CD8+ memory stem cells for the treatment of human B-cell malignancies
    Article Snippet: .. SUDHL4 (diffuse large B-cell lymphoma line), CCRF-CEM (acute lymphoblastic T-cell leukemia line), and NALM6-GL (acute lymphoblastic leukemia line, stably transfected with green fluorescent protein and luciferase) were cultured in RPMI 1640 media supplemented with 10% heat-inactivated fetal bovine serum (Sigma-Aldrich), 100 U/mL penicillin, 100 μg/mL streptomycin, 2 mM glutamax, and 1 mM sodium pyruvate (Thermo Fisher Scientific). .. A detailed list of the antibodies used in flow cytometry experiments can be found in the supplemental Methods.

    Article Title: Amoxicillin Modulates ApoA-I Transcription and Secretion, Predominantly via PPARα Transactivation Inhibition
    Article Snippet: .. Luciferase Assay PPARα transcriptional activity was analyzed by transfection of HepG2 cells using X-treme gene 9 DNA transfection reagent (Sigma, Uithoorn, Netherlands) with the following plasmids: pcDNA3.1, pcDNA3.1_PPARα, pGL3, and pGL3_PPRE as previously described [ ]. .. Following transfection and 48-h antibiotics treatment, cells were harvested by lysis in 1× luciferase lysis buffer (Promega, Madison, USA) and luciferase activity, reflecting PPARα transactivation, and was determined by a GloMax® 96 Microplate luminometer, following the manufacturer’s instructions (Promega, Madison, WI, USA).

    Luciferase:

    Article Title: LncRNA‐Hh Strengthen Cancer Stem Cells Generation in Twist‐Positive Breast Cancer via Activation of Hedgehog Signaling Pathway
    Article Snippet: .. HEK293, MCF‐7/Twist, Hs578T, and BT549 cells were transiently cotransfected with the pGL3‐lncRNA‐Hh‐Luc promoter luciferase plasmid and Twist expression plasmids using Lipofectamine 2000 (Sigma). .. After 48 hours transfection, the cell lysates were prepared, and luciferase activity was assayed according to the instruction of the manufacturer (Promega).

    Article Title: dREAM co-operates with insulator-binding proteins and regulates expression at divergently paired genes
    Article Snippet: .. Drosophila tissue culture and Luciferase reporter assay S2 cells were maintained in Shields and Sang M3 medium (Sigma) with 10% fetal bovine serum, BPYE and 1% Penicillin/Streptomycin. .. For the enhancer-blocking assay, S2 cells were transfected with 100 ng of the enhancer-blocking reporter and 250 ng of the Renilla control plasmid, using the X-tremeGENE HP transfection reagent (Roche).

    Article Title: Generation of clinical-grade CD19-specific CAR-modified CD8+ memory stem cells for the treatment of human B-cell malignancies
    Article Snippet: .. SUDHL4 (diffuse large B-cell lymphoma line), CCRF-CEM (acute lymphoblastic T-cell leukemia line), and NALM6-GL (acute lymphoblastic leukemia line, stably transfected with green fluorescent protein and luciferase) were cultured in RPMI 1640 media supplemented with 10% heat-inactivated fetal bovine serum (Sigma-Aldrich), 100 U/mL penicillin, 100 μg/mL streptomycin, 2 mM glutamax, and 1 mM sodium pyruvate (Thermo Fisher Scientific). .. A detailed list of the antibodies used in flow cytometry experiments can be found in the supplemental Methods.

    Article Title: Sox2 inhibits Wnt-β-catenin signaling and metastatic potency of cisplatin-resistant lung adenocarcinoma cells
    Article Snippet: .. The canonical Wnt reporter plasmid carrying a tandem of 7 T cell factor (TCF) binding sites upstream of a minimal c-fos promoter driving the firefly luciferase gene (BATflash) and its control plasmid (BOTflash, containing mutated TCF binding sites) were produced by EMD Millipore (Billerica, MA, USA). .. The transfection of control plasmid expressing Renilla luciferase (RL) from (Promega Corporation, Madison, WI, USA) was used for assessing the transfection efficiency.

    Article Title: Amoxicillin Modulates ApoA-I Transcription and Secretion, Predominantly via PPARα Transactivation Inhibition
    Article Snippet: .. Luciferase Assay PPARα transcriptional activity was analyzed by transfection of HepG2 cells using X-treme gene 9 DNA transfection reagent (Sigma, Uithoorn, Netherlands) with the following plasmids: pcDNA3.1, pcDNA3.1_PPARα, pGL3, and pGL3_PPRE as previously described [ ]. .. Following transfection and 48-h antibiotics treatment, cells were harvested by lysis in 1× luciferase lysis buffer (Promega, Madison, USA) and luciferase activity, reflecting PPARα transactivation, and was determined by a GloMax® 96 Microplate luminometer, following the manufacturer’s instructions (Promega, Madison, WI, USA).

    Article Title: Novel TMEM173 Mutation and the Role of Disease Modifying Alleles
    Article Snippet: .. Luciferase Assay HEK293 cells were cultured in complete DMEM (Sigma-Aldrich, Espoo, Finland). .. 20,000 cells were seeded onto Costar 3610 white, clear bottom 96-well plates, and the following day co-transfected with 50 ng of TMEM173 constructs or empty C-MAC-tag-vector , 40 ng of IFN-β promoter-driven firefly luciferase reporter plasmid (IFN-β-pGL3; a kind gift from Yanick J.

    Reporter Assay:

    Article Title: dREAM co-operates with insulator-binding proteins and regulates expression at divergently paired genes
    Article Snippet: .. Drosophila tissue culture and Luciferase reporter assay S2 cells were maintained in Shields and Sang M3 medium (Sigma) with 10% fetal bovine serum, BPYE and 1% Penicillin/Streptomycin. .. For the enhancer-blocking assay, S2 cells were transfected with 100 ng of the enhancer-blocking reporter and 250 ng of the Renilla control plasmid, using the X-tremeGENE HP transfection reagent (Roche).

    Stable Transfection:

    Article Title: Generation of clinical-grade CD19-specific CAR-modified CD8+ memory stem cells for the treatment of human B-cell malignancies
    Article Snippet: .. SUDHL4 (diffuse large B-cell lymphoma line), CCRF-CEM (acute lymphoblastic T-cell leukemia line), and NALM6-GL (acute lymphoblastic leukemia line, stably transfected with green fluorescent protein and luciferase) were cultured in RPMI 1640 media supplemented with 10% heat-inactivated fetal bovine serum (Sigma-Aldrich), 100 U/mL penicillin, 100 μg/mL streptomycin, 2 mM glutamax, and 1 mM sodium pyruvate (Thermo Fisher Scientific). .. A detailed list of the antibodies used in flow cytometry experiments can be found in the supplemental Methods.

    Construct:

    Article Title: SIRT1 Alleviates LPS-Induced IL-1β Production by Suppressing NLRP3 Inflammasome Activation and ROS Production in Trophoblasts
    Article Snippet: .. HEK293T cells were transfected with the following lentiviral constructs with packaging plasmids, using polyethylenimine reagent: sh-luciferase (sh-Luc), sh-SIRT1, and sh-NLRP3 constructs (Sigma-Aldrich, St. Louis, MO, USA). .. Lentiviral supernatants were collected and filtered at 48 and 72 h after transfection.

    Produced:

    Article Title: Sox2 inhibits Wnt-β-catenin signaling and metastatic potency of cisplatin-resistant lung adenocarcinoma cells
    Article Snippet: .. The canonical Wnt reporter plasmid carrying a tandem of 7 T cell factor (TCF) binding sites upstream of a minimal c-fos promoter driving the firefly luciferase gene (BATflash) and its control plasmid (BOTflash, containing mutated TCF binding sites) were produced by EMD Millipore (Billerica, MA, USA). .. The transfection of control plasmid expressing Renilla luciferase (RL) from (Promega Corporation, Madison, WI, USA) was used for assessing the transfection efficiency.

    Activity Assay:

    Article Title: Amoxicillin Modulates ApoA-I Transcription and Secretion, Predominantly via PPARα Transactivation Inhibition
    Article Snippet: .. Luciferase Assay PPARα transcriptional activity was analyzed by transfection of HepG2 cells using X-treme gene 9 DNA transfection reagent (Sigma, Uithoorn, Netherlands) with the following plasmids: pcDNA3.1, pcDNA3.1_PPARα, pGL3, and pGL3_PPRE as previously described [ ]. .. Following transfection and 48-h antibiotics treatment, cells were harvested by lysis in 1× luciferase lysis buffer (Promega, Madison, USA) and luciferase activity, reflecting PPARα transactivation, and was determined by a GloMax® 96 Microplate luminometer, following the manufacturer’s instructions (Promega, Madison, WI, USA).

    Cell Culture:

    Article Title: Generation of clinical-grade CD19-specific CAR-modified CD8+ memory stem cells for the treatment of human B-cell malignancies
    Article Snippet: .. SUDHL4 (diffuse large B-cell lymphoma line), CCRF-CEM (acute lymphoblastic T-cell leukemia line), and NALM6-GL (acute lymphoblastic leukemia line, stably transfected with green fluorescent protein and luciferase) were cultured in RPMI 1640 media supplemented with 10% heat-inactivated fetal bovine serum (Sigma-Aldrich), 100 U/mL penicillin, 100 μg/mL streptomycin, 2 mM glutamax, and 1 mM sodium pyruvate (Thermo Fisher Scientific). .. A detailed list of the antibodies used in flow cytometry experiments can be found in the supplemental Methods.

    Article Title: Novel TMEM173 Mutation and the Role of Disease Modifying Alleles
    Article Snippet: .. Luciferase Assay HEK293 cells were cultured in complete DMEM (Sigma-Aldrich, Espoo, Finland). .. 20,000 cells were seeded onto Costar 3610 white, clear bottom 96-well plates, and the following day co-transfected with 50 ng of TMEM173 constructs or empty C-MAC-tag-vector , 40 ng of IFN-β promoter-driven firefly luciferase reporter plasmid (IFN-β-pGL3; a kind gift from Yanick J.

    Expressing:

    Article Title: LncRNA‐Hh Strengthen Cancer Stem Cells Generation in Twist‐Positive Breast Cancer via Activation of Hedgehog Signaling Pathway
    Article Snippet: .. HEK293, MCF‐7/Twist, Hs578T, and BT549 cells were transiently cotransfected with the pGL3‐lncRNA‐Hh‐Luc promoter luciferase plasmid and Twist expression plasmids using Lipofectamine 2000 (Sigma). .. After 48 hours transfection, the cell lysates were prepared, and luciferase activity was assayed according to the instruction of the manufacturer (Promega).

    Binding Assay:

    Article Title: Sox2 inhibits Wnt-β-catenin signaling and metastatic potency of cisplatin-resistant lung adenocarcinoma cells
    Article Snippet: .. The canonical Wnt reporter plasmid carrying a tandem of 7 T cell factor (TCF) binding sites upstream of a minimal c-fos promoter driving the firefly luciferase gene (BATflash) and its control plasmid (BOTflash, containing mutated TCF binding sites) were produced by EMD Millipore (Billerica, MA, USA). .. The transfection of control plasmid expressing Renilla luciferase (RL) from (Promega Corporation, Madison, WI, USA) was used for assessing the transfection efficiency.

    Plasmid Preparation:

    Article Title: LncRNA‐Hh Strengthen Cancer Stem Cells Generation in Twist‐Positive Breast Cancer via Activation of Hedgehog Signaling Pathway
    Article Snippet: .. HEK293, MCF‐7/Twist, Hs578T, and BT549 cells were transiently cotransfected with the pGL3‐lncRNA‐Hh‐Luc promoter luciferase plasmid and Twist expression plasmids using Lipofectamine 2000 (Sigma). .. After 48 hours transfection, the cell lysates were prepared, and luciferase activity was assayed according to the instruction of the manufacturer (Promega).

    Article Title: Sox2 inhibits Wnt-β-catenin signaling and metastatic potency of cisplatin-resistant lung adenocarcinoma cells
    Article Snippet: .. The canonical Wnt reporter plasmid carrying a tandem of 7 T cell factor (TCF) binding sites upstream of a minimal c-fos promoter driving the firefly luciferase gene (BATflash) and its control plasmid (BOTflash, containing mutated TCF binding sites) were produced by EMD Millipore (Billerica, MA, USA). .. The transfection of control plasmid expressing Renilla luciferase (RL) from (Promega Corporation, Madison, WI, USA) was used for assessing the transfection efficiency.

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    Millipore rabbit anti β gal
    JNK signaling is unaffected by changes in Pvr activity. (A-G) Immunostaining of mid-stage dorsal closure embryos for Fas3 (or Pvr) and <t>β-gal</t> protein from the JNK transcriptional reporter puc-lacZ at the LE (arrows). pnr-G4 directs transgene expression
    Rabbit Anti β Gal, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti β gal/product/Millipore
    Average 93 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    rabbit anti β gal - by Bioz Stars, 2020-07
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    90
    Millipore β galactosidase sa β gal activity
    miR-34a induces lung fibroblast senescence. ( A – D ) Human lung fibroblasts MRC-5 were transfected with 50 nM control mimics or mimics for miR-34a. Three days after transfection, senescence–associated <t>β-galactosidase</t> <t>(SA-β-gal)</t> activities in the cells were determined ( A and B ). Levels of p21, plasminogen activator inhibitor-1 (PAI-1), nicotinamide adenine dinucleotide phosphate oxidase 4 (Nox4), and hyaluronan synthase (HAS2) were determined by real-time PCR ( C and D ) ( n = 3; mean ± SD). * P
    β Galactosidase Sa β Gal Activity, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β galactosidase sa β gal activity/product/Millipore
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    β galactosidase sa β gal activity - by Bioz Stars, 2020-07
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    Image Search Results


    JNK signaling is unaffected by changes in Pvr activity. (A-G) Immunostaining of mid-stage dorsal closure embryos for Fas3 (or Pvr) and β-gal protein from the JNK transcriptional reporter puc-lacZ at the LE (arrows). pnr-G4 directs transgene expression

    Journal: Development (Cambridge, England)

    Article Title: The receptor tyrosine kinase Pvr promotes tissue closure by coordinating corpse removal and epidermal zippering

    doi: 10.1242/dev.122226

    Figure Lengend Snippet: JNK signaling is unaffected by changes in Pvr activity. (A-G) Immunostaining of mid-stage dorsal closure embryos for Fas3 (or Pvr) and β-gal protein from the JNK transcriptional reporter puc-lacZ at the LE (arrows). pnr-G4 directs transgene expression

    Article Snippet: Staining reagents were: rat anti-Pvr 1:500 , rat anti-Pvr 1:500 , mouse anti-Fas3 1:40 (7G10, DSHB), presorbed rabbit anti-β-gal 1:1500 (Cappel, 55976), mouse anti-phosphotyrosine 1:1000 (4G10, EMD Millipore, 05-321), Texas Red-phalloidin 1:500 (Life Technologies), and Vectashield with DAPI mounting media (Vector Labs).

    Techniques: Activity Assay, Immunostaining, Expressing

    Effects of chronic pain on Wnt activity in ventral hippocampus. (A) Double-immunostaining of Nestin/β-gal and Western-blotting of β-gal in the ventral hippocampus of sham-injured and SNI-treated Topgal mice at 21 dpi. (B) Western-blotting of β-catenin and Axin2 in the ventral hippocampus of sham-injured and SNI-treated mice at 21 dpi. (C) Double-immunostaining and quantification of Nestin/β-gal in the SVZ of sham-injured and SNI-treated Topgal mice at 21 dpi. Inserts in (A) are magnified typical Nestin/β-gal-positive cells in each group, which were pointed by arrows. Bars = 25 μm in (A) and 50 μm in (C) . Values represent mean ± SE. Unpaired, two tailed Student’s t -tests were performed in (B,C) . * P

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Anxiety Specific Response and Contribution of Active Hippocampal Neural Stem Cells to Chronic Pain Through Wnt/β-Catenin Signaling in Mice

    doi: 10.3389/fnmol.2018.00296

    Figure Lengend Snippet: Effects of chronic pain on Wnt activity in ventral hippocampus. (A) Double-immunostaining of Nestin/β-gal and Western-blotting of β-gal in the ventral hippocampus of sham-injured and SNI-treated Topgal mice at 21 dpi. (B) Western-blotting of β-catenin and Axin2 in the ventral hippocampus of sham-injured and SNI-treated mice at 21 dpi. (C) Double-immunostaining and quantification of Nestin/β-gal in the SVZ of sham-injured and SNI-treated Topgal mice at 21 dpi. Inserts in (A) are magnified typical Nestin/β-gal-positive cells in each group, which were pointed by arrows. Bars = 25 μm in (A) and 50 μm in (C) . Values represent mean ± SE. Unpaired, two tailed Student’s t -tests were performed in (B,C) . * P

    Article Snippet: For immunostaining, the sections were blocked by 0.01 M phosphate buffered saline (PBS) containing 0.3% Triton X-100 and 3% bovine serum albumin (BSA) for 1 h. Primary antibodies were used as following: guinea pig anti-DCX (1:500, Millipore), rat anti-BrdU (1:200, Abcam), rabbit anti-NeuN (1:500, TEMECULA), goat anti-Nestin (1:500, Santa Cruz), rabbit anti-β-gal (1:500, MP), anti-β-catenin (1:200, Millipore).

    Techniques: Activity Assay, Double Immunostaining, Western Blot, Mouse Assay, Two Tailed Test

    Effects of Fluoxetine treatment on the Wnt activity in hippocampus. (A) Double-immunostaining of Nestin/β-gal in sham mice treated with saline (Sham+S), sham mice treated with Fluoxetine (Sham+F), SNI mice treated with saline (SNI+S), and SNI mice treated with Fluoxetine (SNI+F) at 21 dpi. Notice the significant increase of radial Nestin-positive cells in Fluoxetine treated mice. (B) Western-blotting of β-gal and β-catenin in Topgal mice with the following treatments: sham injury plus saline (Sham+S), sham injury plus Fluoxetine (Sham+F), SNI plus saline (SNI+S) and SNI plus Fluoxetine (SNI+F). Notice that Fluoxetine treatment significantly increased the expression level of β-gal and β-catenin in both sham and SNI-treated mice, as compared to corresponding saline controls. S, saline. F, Fluoxetine. Inserts in (A) are typical double-stained cells in each group, which were pointed by arrows. Bars = 50 μm. Values represent mean ± SE. Unpaired, two tailed Student’s t -tests were performed in (A,B) . * P

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Anxiety Specific Response and Contribution of Active Hippocampal Neural Stem Cells to Chronic Pain Through Wnt/β-Catenin Signaling in Mice

    doi: 10.3389/fnmol.2018.00296

    Figure Lengend Snippet: Effects of Fluoxetine treatment on the Wnt activity in hippocampus. (A) Double-immunostaining of Nestin/β-gal in sham mice treated with saline (Sham+S), sham mice treated with Fluoxetine (Sham+F), SNI mice treated with saline (SNI+S), and SNI mice treated with Fluoxetine (SNI+F) at 21 dpi. Notice the significant increase of radial Nestin-positive cells in Fluoxetine treated mice. (B) Western-blotting of β-gal and β-catenin in Topgal mice with the following treatments: sham injury plus saline (Sham+S), sham injury plus Fluoxetine (Sham+F), SNI plus saline (SNI+S) and SNI plus Fluoxetine (SNI+F). Notice that Fluoxetine treatment significantly increased the expression level of β-gal and β-catenin in both sham and SNI-treated mice, as compared to corresponding saline controls. S, saline. F, Fluoxetine. Inserts in (A) are typical double-stained cells in each group, which were pointed by arrows. Bars = 50 μm. Values represent mean ± SE. Unpaired, two tailed Student’s t -tests were performed in (A,B) . * P

    Article Snippet: For immunostaining, the sections were blocked by 0.01 M phosphate buffered saline (PBS) containing 0.3% Triton X-100 and 3% bovine serum albumin (BSA) for 1 h. Primary antibodies were used as following: guinea pig anti-DCX (1:500, Millipore), rat anti-BrdU (1:200, Abcam), rabbit anti-NeuN (1:500, TEMECULA), goat anti-Nestin (1:500, Santa Cruz), rabbit anti-β-gal (1:500, MP), anti-β-catenin (1:200, Millipore).

    Techniques: Activity Assay, Double Immunostaining, Mouse Assay, Western Blot, Expressing, Staining, Two Tailed Test

    Effects of chronic pain on Wnt activity in ventral hippocampus. (A) Double-immunostaining of Nestin/β-gal and Western-blotting of β-gal in the ventral hippocampus of sham-injured and SNI-treated Topgal mice at 21 dpi. (B) Western-blotting of β-catenin and Axin2 in the ventral hippocampus of sham-injured and SNI-treated mice at 21 dpi. (C) Double-immunostaining and quantification of Nestin/β-gal in the SVZ of sham-injured and SNI-treated Topgal mice at 21 dpi. Inserts in (A) are magnified typical Nestin/β-gal-positive cells in each group, which were pointed by arrows. Bars = 25 μm in (A) and 50 μm in (C) . Values represent mean ± SE. Unpaired, two tailed Student’s t -tests were performed in (B,C) . * P

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Anxiety Specific Response and Contribution of Active Hippocampal Neural Stem Cells to Chronic Pain Through Wnt/β-Catenin Signaling in Mice

    doi: 10.3389/fnmol.2018.00296

    Figure Lengend Snippet: Effects of chronic pain on Wnt activity in ventral hippocampus. (A) Double-immunostaining of Nestin/β-gal and Western-blotting of β-gal in the ventral hippocampus of sham-injured and SNI-treated Topgal mice at 21 dpi. (B) Western-blotting of β-catenin and Axin2 in the ventral hippocampus of sham-injured and SNI-treated mice at 21 dpi. (C) Double-immunostaining and quantification of Nestin/β-gal in the SVZ of sham-injured and SNI-treated Topgal mice at 21 dpi. Inserts in (A) are magnified typical Nestin/β-gal-positive cells in each group, which were pointed by arrows. Bars = 25 μm in (A) and 50 μm in (C) . Values represent mean ± SE. Unpaired, two tailed Student’s t -tests were performed in (B,C) . * P

    Article Snippet: For immunostaining, the sections were blocked by 0.01 M phosphate buffered saline (PBS) containing 0.3% Triton X-100 and 3% bovine serum albumin (BSA) for 1 h. Primary antibodies were used as following: guinea pig anti-DCX (1:500, Millipore), rat anti-BrdU (1:200, Abcam), rabbit anti-NeuN (1:500, TEMECULA), goat anti-Nestin (1:500, Santa Cruz), rabbit anti-β-gal (1:500, MP), anti-β-catenin (1:200, Millipore).

    Techniques: Activity Assay, Double Immunostaining, Western Blot, Mouse Assay, Two Tailed Test

    Effects of Fluoxetine treatment on the Wnt activity in hippocampus. (A) Double-immunostaining of Nestin/β-gal in sham mice treated with saline (Sham+S), sham mice treated with Fluoxetine (Sham+F), SNI mice treated with saline (SNI+S), and SNI mice treated with Fluoxetine (SNI+F) at 21 dpi. Notice the significant increase of radial Nestin-positive cells in Fluoxetine treated mice. (B) Western-blotting of β-gal and β-catenin in Topgal mice with the following treatments: sham injury plus saline (Sham+S), sham injury plus Fluoxetine (Sham+F), SNI plus saline (SNI+S) and SNI plus Fluoxetine (SNI+F). Notice that Fluoxetine treatment significantly increased the expression level of β-gal and β-catenin in both sham and SNI-treated mice, as compared to corresponding saline controls. S, saline. F, Fluoxetine. Inserts in (A) are typical double-stained cells in each group, which were pointed by arrows. Bars = 50 μm. Values represent mean ± SE. Unpaired, two tailed Student’s t -tests were performed in (A,B) . * P

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Anxiety Specific Response and Contribution of Active Hippocampal Neural Stem Cells to Chronic Pain Through Wnt/β-Catenin Signaling in Mice

    doi: 10.3389/fnmol.2018.00296

    Figure Lengend Snippet: Effects of Fluoxetine treatment on the Wnt activity in hippocampus. (A) Double-immunostaining of Nestin/β-gal in sham mice treated with saline (Sham+S), sham mice treated with Fluoxetine (Sham+F), SNI mice treated with saline (SNI+S), and SNI mice treated with Fluoxetine (SNI+F) at 21 dpi. Notice the significant increase of radial Nestin-positive cells in Fluoxetine treated mice. (B) Western-blotting of β-gal and β-catenin in Topgal mice with the following treatments: sham injury plus saline (Sham+S), sham injury plus Fluoxetine (Sham+F), SNI plus saline (SNI+S) and SNI plus Fluoxetine (SNI+F). Notice that Fluoxetine treatment significantly increased the expression level of β-gal and β-catenin in both sham and SNI-treated mice, as compared to corresponding saline controls. S, saline. F, Fluoxetine. Inserts in (A) are typical double-stained cells in each group, which were pointed by arrows. Bars = 50 μm. Values represent mean ± SE. Unpaired, two tailed Student’s t -tests were performed in (A,B) . * P

    Article Snippet: For immunostaining, the sections were blocked by 0.01 M phosphate buffered saline (PBS) containing 0.3% Triton X-100 and 3% bovine serum albumin (BSA) for 1 h. Primary antibodies were used as following: guinea pig anti-DCX (1:500, Millipore), rat anti-BrdU (1:200, Abcam), rabbit anti-NeuN (1:500, TEMECULA), goat anti-Nestin (1:500, Santa Cruz), rabbit anti-β-gal (1:500, MP), anti-β-catenin (1:200, Millipore).

    Techniques: Activity Assay, Double Immunostaining, Mouse Assay, Western Blot, Expressing, Staining, Two Tailed Test

    miR-34a induces lung fibroblast senescence. ( A – D ) Human lung fibroblasts MRC-5 were transfected with 50 nM control mimics or mimics for miR-34a. Three days after transfection, senescence–associated β-galactosidase (SA-β-gal) activities in the cells were determined ( A and B ). Levels of p21, plasminogen activator inhibitor-1 (PAI-1), nicotinamide adenine dinucleotide phosphate oxidase 4 (Nox4), and hyaluronan synthase (HAS2) were determined by real-time PCR ( C and D ) ( n = 3; mean ± SD). * P

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: miR-34a Inhibits Lung Fibrosis by Inducing Lung Fibroblast Senescence

    doi: 10.1165/rcmb.2016-0163OC

    Figure Lengend Snippet: miR-34a induces lung fibroblast senescence. ( A – D ) Human lung fibroblasts MRC-5 were transfected with 50 nM control mimics or mimics for miR-34a. Three days after transfection, senescence–associated β-galactosidase (SA-β-gal) activities in the cells were determined ( A and B ). Levels of p21, plasminogen activator inhibitor-1 (PAI-1), nicotinamide adenine dinucleotide phosphate oxidase 4 (Nox4), and hyaluronan synthase (HAS2) were determined by real-time PCR ( C and D ) ( n = 3; mean ± SD). * P

    Article Snippet: Cellular senescence was evaluated by determining cellular senescence–associated β-galactosidase (SA-β-gal) activity with either the Cellular Senescence Assay Kit (Millipore, Temecula, CA) or the MarkerGene Cellular Senescence Microtiterplate Assay Kit (Marker Gene Technologies, Eugene, OR) according to the manufacturer’s instructions.

    Techniques: Transfection, Real-time Polymerase Chain Reaction

    miR-34a knockdown inhibits senescence of lung fibroblasts. ( A and B ) Human lung fibroblasts MRC-5 were treated with low-dose bleomycin (0.01 U/ml) for 2 days. ( A ) SA-β-gal activities in the cells were measured, and ( B ) levels of miR-34a were determined by real-time PCR ( n = 3; mean ± SD). ** P

    Journal: American Journal of Respiratory Cell and Molecular Biology

    Article Title: miR-34a Inhibits Lung Fibrosis by Inducing Lung Fibroblast Senescence

    doi: 10.1165/rcmb.2016-0163OC

    Figure Lengend Snippet: miR-34a knockdown inhibits senescence of lung fibroblasts. ( A and B ) Human lung fibroblasts MRC-5 were treated with low-dose bleomycin (0.01 U/ml) for 2 days. ( A ) SA-β-gal activities in the cells were measured, and ( B ) levels of miR-34a were determined by real-time PCR ( n = 3; mean ± SD). ** P

    Article Snippet: Cellular senescence was evaluated by determining cellular senescence–associated β-galactosidase (SA-β-gal) activity with either the Cellular Senescence Assay Kit (Millipore, Temecula, CA) or the MarkerGene Cellular Senescence Microtiterplate Assay Kit (Marker Gene Technologies, Eugene, OR) according to the manufacturer’s instructions.

    Techniques: Real-time Polymerase Chain Reaction