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Dalian Huali Jingang Pharmaceutical Co Ltd β elemene
<t>β-elemene</t> inhibits the invasion and migration of SiHa cells. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30 and 40 µg/ml) for 24 and 72 h time intervals, motility and invasion assayswere performed (magnification, ×100). (B) Statistical analysis demonstrating the quantification of the number of invaded SiHa cellsfrom the motility and invasion assays; *P
β Elemene, supplied by Dalian Huali Jingang Pharmaceutical Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/β elemene/product/Dalian Huali Jingang Pharmaceutical Co Ltd
Average 93 stars, based on 3 article reviews
Price from $9.99 to $1999.99
β elemene - by Bioz Stars, 2020-09
93/100 stars

Images

1) Product Images from "Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells"

Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2018.8455

β-elemene inhibits the invasion and migration of SiHa cells. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30 and 40 µg/ml) for 24 and 72 h time intervals, motility and invasion assayswere performed (magnification, ×100). (B) Statistical analysis demonstrating the quantification of the number of invaded SiHa cellsfrom the motility and invasion assays; *P
Figure Legend Snippet: β-elemene inhibits the invasion and migration of SiHa cells. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30 and 40 µg/ml) for 24 and 72 h time intervals, motility and invasion assayswere performed (magnification, ×100). (B) Statistical analysis demonstrating the quantification of the number of invaded SiHa cellsfrom the motility and invasion assays; *P

Techniques Used: Migration

β-elemene induces apoptosis in SiHa cells. (A) Following treatment of SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to detect cellular apoptosis; *P
Figure Legend Snippet: β-elemene induces apoptosis in SiHa cells. (A) Following treatment of SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to detect cellular apoptosis; *P

Techniques Used: Flow Cytometry, Cytometry

β-elemene inhibits the proliferation and survival of SiHa cells. Following treatment of SiHa cells with increasing doses of β-elemene (0–50 µg/ml) for 24, 48 and 72 h, the MTT assay was used to detect the proliferation and survival of SiHa cells. Each test was conducted in triplicate. Values presented represent the mean ± standard deviation of the three independent experiments. *P
Figure Legend Snippet: β-elemene inhibits the proliferation and survival of SiHa cells. Following treatment of SiHa cells with increasing doses of β-elemene (0–50 µg/ml) for 24, 48 and 72 h, the MTT assay was used to detect the proliferation and survival of SiHa cells. Each test was conducted in triplicate. Values presented represent the mean ± standard deviation of the three independent experiments. *P

Techniques Used: MTT Assay, Standard Deviation

β-elemene induces cell cycle arrest in SiHa cells during G1. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to investigate the influence of β-elemene on the progression of the cell cycle; *P
Figure Legend Snippet: β-elemene induces cell cycle arrest in SiHa cells during G1. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to investigate the influence of β-elemene on the progression of the cell cycle; *P

Techniques Used: Flow Cytometry, Cytometry

β-elemene suppresses the Wnt/β-catenin signaling pathway. Expression levels of β-catenin, TCF7, and c-Myc were detected via western blot analysis. Each experiment was performed in triplicate. Values presented represent the mean ± standard deviation of three independent experiments. *P
Figure Legend Snippet: β-elemene suppresses the Wnt/β-catenin signaling pathway. Expression levels of β-catenin, TCF7, and c-Myc were detected via western blot analysis. Each experiment was performed in triplicate. Values presented represent the mean ± standard deviation of three independent experiments. *P

Techniques Used: Expressing, Western Blot, Standard Deviation

2) Product Images from "Preliminary evaluation of the potential role of β-elemene in reversing erlotinib-resistant human NSCLC A549/ER cells"

Article Title: Preliminary evaluation of the potential role of β-elemene in reversing erlotinib-resistant human NSCLC A549/ER cells

Journal: Oncology Letters

doi: 10.3892/ol.2018.8980

β-ELE increases intracellular accumulation of Rh123 in A549/ER cells. The MFI of intracellular Rh123 was detected by flow cytometry, indirectly reflecting the function of P-gp following exposure of A549/ER cells to 15 µg/ml β-ELE for 24 h. (A) Control group; (B) experimental group (15 µg/ml β-ELE). β-ELE, β-elemene; Rh123, rhodamine 123; ER, erlotinib resistant; MFI, mean fluorescence intensity; P-gp, P-glycoprotein.
Figure Legend Snippet: β-ELE increases intracellular accumulation of Rh123 in A549/ER cells. The MFI of intracellular Rh123 was detected by flow cytometry, indirectly reflecting the function of P-gp following exposure of A549/ER cells to 15 µg/ml β-ELE for 24 h. (A) Control group; (B) experimental group (15 µg/ml β-ELE). β-ELE, β-elemene; Rh123, rhodamine 123; ER, erlotinib resistant; MFI, mean fluorescence intensity; P-gp, P-glycoprotein.

Techniques Used: Flow Cytometry, Cytometry, Fluorescence

3) Product Images from "Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells"

Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2018.8455

β-elemene inhibits the invasion and migration of SiHa cells. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30 and 40 µg/ml) for 24 and 72 h time intervals, motility and invasion assayswere performed (magnification, ×100). (B) Statistical analysis demonstrating the quantification of the number of invaded SiHa cellsfrom the motility and invasion assays; *P
Figure Legend Snippet: β-elemene inhibits the invasion and migration of SiHa cells. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30 and 40 µg/ml) for 24 and 72 h time intervals, motility and invasion assayswere performed (magnification, ×100). (B) Statistical analysis demonstrating the quantification of the number of invaded SiHa cellsfrom the motility and invasion assays; *P

Techniques Used: Migration

β-elemene induces apoptosis in SiHa cells. (A) Following treatment of SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to detect cellular apoptosis; *P
Figure Legend Snippet: β-elemene induces apoptosis in SiHa cells. (A) Following treatment of SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to detect cellular apoptosis; *P

Techniques Used: Flow Cytometry, Cytometry

β-elemene inhibits the proliferation and survival of SiHa cells. Following treatment of SiHa cells with increasing doses of β-elemene (0–50 µg/ml) for 24, 48 and 72 h, the MTT assay was used to detect the proliferation and survival of SiHa cells. Each test was conducted in triplicate. Values presented represent the mean ± standard deviation of the three independent experiments. *P
Figure Legend Snippet: β-elemene inhibits the proliferation and survival of SiHa cells. Following treatment of SiHa cells with increasing doses of β-elemene (0–50 µg/ml) for 24, 48 and 72 h, the MTT assay was used to detect the proliferation and survival of SiHa cells. Each test was conducted in triplicate. Values presented represent the mean ± standard deviation of the three independent experiments. *P

Techniques Used: MTT Assay, Standard Deviation

β-elemene induces cell cycle arrest in SiHa cells during G1. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to investigate the influence of β-elemene on the progression of the cell cycle; *P
Figure Legend Snippet: β-elemene induces cell cycle arrest in SiHa cells during G1. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to investigate the influence of β-elemene on the progression of the cell cycle; *P

Techniques Used: Flow Cytometry, Cytometry

β-elemene suppresses the Wnt/β-catenin signaling pathway. Expression levels of β-catenin, TCF7, and c-Myc were detected via western blot analysis. Each experiment was performed in triplicate. Values presented represent the mean ± standard deviation of three independent experiments. *P
Figure Legend Snippet: β-elemene suppresses the Wnt/β-catenin signaling pathway. Expression levels of β-catenin, TCF7, and c-Myc were detected via western blot analysis. Each experiment was performed in triplicate. Values presented represent the mean ± standard deviation of three independent experiments. *P

Techniques Used: Expressing, Western Blot, Standard Deviation

Related Articles

Lysis:

Article Title: Preliminary evaluation of the potential role of β-elemene in reversing erlotinib-resistant human NSCLC A549/ER cells
Article Snippet: .. The following reagents were used in the present study: β-elemene (Dalian Huali Jingang Pharmaceutical Co., Ltd. Dalian, China); erlotinib (Roche Applied Science, Penzburg, China); Roswell Park Memorial Institute (RPMI)-1640 culture medium and fetal bovine serum (FBS) (both Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA); penicillin (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany); Streptomycin (Sigma-Aldrich; Merck KGaA); radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology Co., Ltd., Haimen, China); MTT cell proliferation assay kits (Ameresco, Inc., Framingham, MA, USA); dimethyl sulfoxide (DMSO; Shanghai Biological Engineering Co., Ltd., Shanghai, China); Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double dye kits (EMD Millipore, Billerica, MA, USA); enhanced chemiluminescence (ECL) reagent kits, PI and Rhodamine 123 (Rh123) (Sigma-Aldrich; Merck KGaA); mouse anti-human monoclonal antibodies against P-gp (Abcam, Cambridge, UK; cat. no., ab10333), β-actin (Abcam; cat. no., ab11003) and horseradish peroxidase (HRP)-labeled goat anti-mouse immunoglobulin (IgG) (Abcam; cat. no., ab6789). .. Inverted microscope (Olympus); Western blot transfer system, Western blot electrophoresis apparatus, Microplate Reader Model 680 and Gel imaging system (Bio-Rad Laboratories, Inc., Hercules, CA, USA); and flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).

Proliferation Assay:

Article Title: Preliminary evaluation of the potential role of β-elemene in reversing erlotinib-resistant human NSCLC A549/ER cells
Article Snippet: .. The following reagents were used in the present study: β-elemene (Dalian Huali Jingang Pharmaceutical Co., Ltd. Dalian, China); erlotinib (Roche Applied Science, Penzburg, China); Roswell Park Memorial Institute (RPMI)-1640 culture medium and fetal bovine serum (FBS) (both Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA); penicillin (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany); Streptomycin (Sigma-Aldrich; Merck KGaA); radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology Co., Ltd., Haimen, China); MTT cell proliferation assay kits (Ameresco, Inc., Framingham, MA, USA); dimethyl sulfoxide (DMSO; Shanghai Biological Engineering Co., Ltd., Shanghai, China); Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double dye kits (EMD Millipore, Billerica, MA, USA); enhanced chemiluminescence (ECL) reagent kits, PI and Rhodamine 123 (Rh123) (Sigma-Aldrich; Merck KGaA); mouse anti-human monoclonal antibodies against P-gp (Abcam, Cambridge, UK; cat. no., ab10333), β-actin (Abcam; cat. no., ab11003) and horseradish peroxidase (HRP)-labeled goat anti-mouse immunoglobulin (IgG) (Abcam; cat. no., ab6789). .. Inverted microscope (Olympus); Western blot transfer system, Western blot electrophoresis apparatus, Microplate Reader Model 680 and Gel imaging system (Bio-Rad Laboratories, Inc., Hercules, CA, USA); and flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).

Radio Immunoprecipitation:

Article Title: Preliminary evaluation of the potential role of β-elemene in reversing erlotinib-resistant human NSCLC A549/ER cells
Article Snippet: .. The following reagents were used in the present study: β-elemene (Dalian Huali Jingang Pharmaceutical Co., Ltd. Dalian, China); erlotinib (Roche Applied Science, Penzburg, China); Roswell Park Memorial Institute (RPMI)-1640 culture medium and fetal bovine serum (FBS) (both Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA); penicillin (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany); Streptomycin (Sigma-Aldrich; Merck KGaA); radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology Co., Ltd., Haimen, China); MTT cell proliferation assay kits (Ameresco, Inc., Framingham, MA, USA); dimethyl sulfoxide (DMSO; Shanghai Biological Engineering Co., Ltd., Shanghai, China); Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double dye kits (EMD Millipore, Billerica, MA, USA); enhanced chemiluminescence (ECL) reagent kits, PI and Rhodamine 123 (Rh123) (Sigma-Aldrich; Merck KGaA); mouse anti-human monoclonal antibodies against P-gp (Abcam, Cambridge, UK; cat. no., ab10333), β-actin (Abcam; cat. no., ab11003) and horseradish peroxidase (HRP)-labeled goat anti-mouse immunoglobulin (IgG) (Abcam; cat. no., ab6789). .. Inverted microscope (Olympus); Western blot transfer system, Western blot electrophoresis apparatus, Microplate Reader Model 680 and Gel imaging system (Bio-Rad Laboratories, Inc., Hercules, CA, USA); and flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).

MTT Assay:

Article Title: Preliminary evaluation of the potential role of β-elemene in reversing erlotinib-resistant human NSCLC A549/ER cells
Article Snippet: .. The following reagents were used in the present study: β-elemene (Dalian Huali Jingang Pharmaceutical Co., Ltd. Dalian, China); erlotinib (Roche Applied Science, Penzburg, China); Roswell Park Memorial Institute (RPMI)-1640 culture medium and fetal bovine serum (FBS) (both Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA); penicillin (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany); Streptomycin (Sigma-Aldrich; Merck KGaA); radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology Co., Ltd., Haimen, China); MTT cell proliferation assay kits (Ameresco, Inc., Framingham, MA, USA); dimethyl sulfoxide (DMSO; Shanghai Biological Engineering Co., Ltd., Shanghai, China); Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double dye kits (EMD Millipore, Billerica, MA, USA); enhanced chemiluminescence (ECL) reagent kits, PI and Rhodamine 123 (Rh123) (Sigma-Aldrich; Merck KGaA); mouse anti-human monoclonal antibodies against P-gp (Abcam, Cambridge, UK; cat. no., ab10333), β-actin (Abcam; cat. no., ab11003) and horseradish peroxidase (HRP)-labeled goat anti-mouse immunoglobulin (IgG) (Abcam; cat. no., ab6789). .. Inverted microscope (Olympus); Western blot transfer system, Western blot electrophoresis apparatus, Microplate Reader Model 680 and Gel imaging system (Bio-Rad Laboratories, Inc., Hercules, CA, USA); and flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).

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    Dalian Huali Jingang Pharmaceutical Co Ltd β elemene
    <t>β-elemene</t> inhibits the invasion and migration of SiHa cells. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30 and 40 µg/ml) for 24 and 72 h time intervals, motility and invasion assayswere performed (magnification, ×100). (B) Statistical analysis demonstrating the quantification of the number of invaded SiHa cellsfrom the motility and invasion assays; *P
    β Elemene, supplied by Dalian Huali Jingang Pharmaceutical Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β elemene/product/Dalian Huali Jingang Pharmaceutical Co Ltd
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    β elemene - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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    β-elemene inhibits the invasion and migration of SiHa cells. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30 and 40 µg/ml) for 24 and 72 h time intervals, motility and invasion assayswere performed (magnification, ×100). (B) Statistical analysis demonstrating the quantification of the number of invaded SiHa cellsfrom the motility and invasion assays; *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene inhibits the invasion and migration of SiHa cells. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30 and 40 µg/ml) for 24 and 72 h time intervals, motility and invasion assayswere performed (magnification, ×100). (B) Statistical analysis demonstrating the quantification of the number of invaded SiHa cellsfrom the motility and invasion assays; *P

    Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: Migration

    β-elemene induces apoptosis in SiHa cells. (A) Following treatment of SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to detect cellular apoptosis; *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene induces apoptosis in SiHa cells. (A) Following treatment of SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to detect cellular apoptosis; *P

    Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: Flow Cytometry, Cytometry

    β-elemene inhibits the proliferation and survival of SiHa cells. Following treatment of SiHa cells with increasing doses of β-elemene (0–50 µg/ml) for 24, 48 and 72 h, the MTT assay was used to detect the proliferation and survival of SiHa cells. Each test was conducted in triplicate. Values presented represent the mean ± standard deviation of the three independent experiments. *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene inhibits the proliferation and survival of SiHa cells. Following treatment of SiHa cells with increasing doses of β-elemene (0–50 µg/ml) for 24, 48 and 72 h, the MTT assay was used to detect the proliferation and survival of SiHa cells. Each test was conducted in triplicate. Values presented represent the mean ± standard deviation of the three independent experiments. *P

    Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: MTT Assay, Standard Deviation

    β-elemene induces cell cycle arrest in SiHa cells during G1. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to investigate the influence of β-elemene on the progression of the cell cycle; *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene induces cell cycle arrest in SiHa cells during G1. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to investigate the influence of β-elemene on the progression of the cell cycle; *P

    Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: Flow Cytometry, Cytometry

    β-elemene suppresses the Wnt/β-catenin signaling pathway. Expression levels of β-catenin, TCF7, and c-Myc were detected via western blot analysis. Each experiment was performed in triplicate. Values presented represent the mean ± standard deviation of three independent experiments. *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene suppresses the Wnt/β-catenin signaling pathway. Expression levels of β-catenin, TCF7, and c-Myc were detected via western blot analysis. Each experiment was performed in triplicate. Values presented represent the mean ± standard deviation of three independent experiments. *P

    Article Snippet: Chemicals and reagents SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: Expressing, Western Blot, Standard Deviation

    β-ELE increases intracellular accumulation of Rh123 in A549/ER cells. The MFI of intracellular Rh123 was detected by flow cytometry, indirectly reflecting the function of P-gp following exposure of A549/ER cells to 15 µg/ml β-ELE for 24 h. (A) Control group; (B) experimental group (15 µg/ml β-ELE). β-ELE, β-elemene; Rh123, rhodamine 123; ER, erlotinib resistant; MFI, mean fluorescence intensity; P-gp, P-glycoprotein.

    Journal: Oncology Letters

    Article Title: Preliminary evaluation of the potential role of β-elemene in reversing erlotinib-resistant human NSCLC A549/ER cells

    doi: 10.3892/ol.2018.8980

    Figure Lengend Snippet: β-ELE increases intracellular accumulation of Rh123 in A549/ER cells. The MFI of intracellular Rh123 was detected by flow cytometry, indirectly reflecting the function of P-gp following exposure of A549/ER cells to 15 µg/ml β-ELE for 24 h. (A) Control group; (B) experimental group (15 µg/ml β-ELE). β-ELE, β-elemene; Rh123, rhodamine 123; ER, erlotinib resistant; MFI, mean fluorescence intensity; P-gp, P-glycoprotein.

    Article Snippet: The following reagents were used in the present study: β-elemene (Dalian Huali Jingang Pharmaceutical Co., Ltd. Dalian, China); erlotinib (Roche Applied Science, Penzburg, China); Roswell Park Memorial Institute (RPMI)-1640 culture medium and fetal bovine serum (FBS) (both Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA); penicillin (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany); Streptomycin (Sigma-Aldrich; Merck KGaA); radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology Co., Ltd., Haimen, China); MTT cell proliferation assay kits (Ameresco, Inc., Framingham, MA, USA); dimethyl sulfoxide (DMSO; Shanghai Biological Engineering Co., Ltd., Shanghai, China); Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double dye kits (EMD Millipore, Billerica, MA, USA); enhanced chemiluminescence (ECL) reagent kits, PI and Rhodamine 123 (Rh123) (Sigma-Aldrich; Merck KGaA); mouse anti-human monoclonal antibodies against P-gp (Abcam, Cambridge, UK; cat. no., ab10333), β-actin (Abcam; cat. no., ab11003) and horseradish peroxidase (HRP)-labeled goat anti-mouse immunoglobulin (IgG) (Abcam; cat. no., ab6789).

    Techniques: Flow Cytometry, Cytometry, Fluorescence

    β-elemene inhibits the invasion and migration of SiHa cells. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30 and 40 µg/ml) for 24 and 72 h time intervals, motility and invasion assayswere performed (magnification, ×100). (B) Statistical analysis demonstrating the quantification of the number of invaded SiHa cellsfrom the motility and invasion assays; *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene inhibits the invasion and migration of SiHa cells. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30 and 40 µg/ml) for 24 and 72 h time intervals, motility and invasion assayswere performed (magnification, ×100). (B) Statistical analysis demonstrating the quantification of the number of invaded SiHa cellsfrom the motility and invasion assays; *P

    Article Snippet: SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: Migration

    β-elemene induces apoptosis in SiHa cells. (A) Following treatment of SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to detect cellular apoptosis; *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene induces apoptosis in SiHa cells. (A) Following treatment of SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to detect cellular apoptosis; *P

    Article Snippet: SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: Flow Cytometry, Cytometry

    β-elemene inhibits the proliferation and survival of SiHa cells. Following treatment of SiHa cells with increasing doses of β-elemene (0–50 µg/ml) for 24, 48 and 72 h, the MTT assay was used to detect the proliferation and survival of SiHa cells. Each test was conducted in triplicate. Values presented represent the mean ± standard deviation of the three independent experiments. *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene inhibits the proliferation and survival of SiHa cells. Following treatment of SiHa cells with increasing doses of β-elemene (0–50 µg/ml) for 24, 48 and 72 h, the MTT assay was used to detect the proliferation and survival of SiHa cells. Each test was conducted in triplicate. Values presented represent the mean ± standard deviation of the three independent experiments. *P

    Article Snippet: SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: MTT Assay, Standard Deviation

    β-elemene induces cell cycle arrest in SiHa cells during G1. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to investigate the influence of β-elemene on the progression of the cell cycle; *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene induces cell cycle arrest in SiHa cells during G1. (A) Following treatmentof SiHa cells with increasing doses of β-elemene (0, 20, 30, and 40 µg/ml) for 48 h, flow cytometry was performed in order to investigate the influence of β-elemene on the progression of the cell cycle; *P

    Article Snippet: SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: Flow Cytometry, Cytometry

    β-elemene suppresses the Wnt/β-catenin signaling pathway. Expression levels of β-catenin, TCF7, and c-Myc were detected via western blot analysis. Each experiment was performed in triplicate. Values presented represent the mean ± standard deviation of three independent experiments. *P

    Journal: Molecular Medicine Reports

    Article Title: Therapeutic effects of β-elemene via attenuation of the Wnt/β-catenin signaling pathway in cervical cancer cells

    doi: 10.3892/mmr.2018.8455

    Figure Lengend Snippet: β-elemene suppresses the Wnt/β-catenin signaling pathway. Expression levels of β-catenin, TCF7, and c-Myc were detected via western blot analysis. Each experiment was performed in triplicate. Values presented represent the mean ± standard deviation of three independent experiments. *P

    Article Snippet: SiHa cells were obtained from the American Type Culture Collection (Manassas, VA, USA). β-elemene was obtained from Dalian Huali JinGang Pharmaceutical Co., Ltd. (Dalian, China) and dissolved in PBS in order to generate a 5 mg/ml stock solution for experimental use.

    Techniques: Expressing, Western Blot, Standard Deviation