β elemene  (Abcam)

 
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    Name:
    β Elemene
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    Catalog Number:
    ab145171
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    Structured Review

    Abcam β elemene
    Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- <t>β</t> -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p

    https://www.bioz.com/result/β elemene/product/Abcam
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    β elemene - by Bioz Stars, 2020-09
    91/100 stars

    Images

    1) Product Images from "Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite"

    Article Title: Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite

    Journal: Proceedings of the Royal Society B: Biological Sciences

    doi: 10.1098/rspb.2017.1134

    Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- β -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p
    Figure Legend Snippet: Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- β -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p

    Techniques Used: Activity Assay

    GC–MS results. ( a ) Gas chromatographs of the whole-body extracts of both sexes of soldiers and workers from the same colony (UR160505A). The compound associated with a single large peak (indicated by an arrow) was identified as (−)- β -elemene (retention time: 16.41 min in male, 16.41 min in female). ( b ) Mass spectra of (−)- β -elemene from male soldiers (upper) and of commercial standard (lower). ( c ) Mass spectra of (−)- β -elemene from female soldiers (upper) and of commercial standard (lower). (Online version in colour.)
    Figure Legend Snippet: GC–MS results. ( a ) Gas chromatographs of the whole-body extracts of both sexes of soldiers and workers from the same colony (UR160505A). The compound associated with a single large peak (indicated by an arrow) was identified as (−)- β -elemene (retention time: 16.41 min in male, 16.41 min in female). ( b ) Mass spectra of (−)- β -elemene from male soldiers (upper) and of commercial standard (lower). ( c ) Mass spectra of (−)- β -elemene from female soldiers (upper) and of commercial standard (lower). (Online version in colour.)

    Techniques Used: Gas Chromatography-Mass Spectrometry

    Antifungal activity test of soldier pheromone component. ( a ) Schematic drawing of fungal bioassay device. PDA, potato-dextrose agar. ( b – d ) Effects of (−)- β -elemene on the mycelial growth of fungi B. bassiana ( b ), Me. anisopliae ( c ) and termite ball fungi (TMB) ( d ). (−)- β -elemene suppressed the growth of entomopathogenic fungi B. bassiana and Me. anisopliae , but enhanced that of TMB (repeated-measures ANOVA; * p
    Figure Legend Snippet: Antifungal activity test of soldier pheromone component. ( a ) Schematic drawing of fungal bioassay device. PDA, potato-dextrose agar. ( b – d ) Effects of (−)- β -elemene on the mycelial growth of fungi B. bassiana ( b ), Me. anisopliae ( c ) and termite ball fungi (TMB) ( d ). (−)- β -elemene suppressed the growth of entomopathogenic fungi B. bassiana and Me. anisopliae , but enhanced that of TMB (repeated-measures ANOVA; * p

    Techniques Used: Activity Assay

    Inhibitory effects of the crude soldier extract and the authentic (−)- β -elemene on soldier differentiation in the presence of JHA, hydroprene. Results show the average of cumulative rate of presoldiers after 15 days, and values denote the mean ± s.e.m. of 20 replications (10 replications × 2 colonies). Soldier extract and (−)- β -elemene suppressed the differentiation of new presoldiers. Different letters mean significant differences (Tukey's HSD test, p
    Figure Legend Snippet: Inhibitory effects of the crude soldier extract and the authentic (−)- β -elemene on soldier differentiation in the presence of JHA, hydroprene. Results show the average of cumulative rate of presoldiers after 15 days, and values denote the mean ± s.e.m. of 20 replications (10 replications × 2 colonies). Soldier extract and (−)- β -elemene suppressed the differentiation of new presoldiers. Different letters mean significant differences (Tukey's HSD test, p

    Techniques Used:

    Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- β -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p
    Figure Legend Snippet: Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- β -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p

    Techniques Used: Activity Assay

    GC–MS results. ( a ) Gas chromatographs of the whole-body extracts of both sexes of soldiers and workers from the same colony (UR160505A). The compound associated with a single large peak (indicated by an arrow) was identified as (−)- β -elemene (retention time: 16.41 min in male, 16.41 min in female). ( b ) Mass spectra of (−)- β -elemene from male soldiers (upper) and of commercial standard (lower). ( c ) Mass spectra of (−)- β -elemene from female soldiers (upper) and of commercial standard (lower). (Online version in colour.)
    Figure Legend Snippet: GC–MS results. ( a ) Gas chromatographs of the whole-body extracts of both sexes of soldiers and workers from the same colony (UR160505A). The compound associated with a single large peak (indicated by an arrow) was identified as (−)- β -elemene (retention time: 16.41 min in male, 16.41 min in female). ( b ) Mass spectra of (−)- β -elemene from male soldiers (upper) and of commercial standard (lower). ( c ) Mass spectra of (−)- β -elemene from female soldiers (upper) and of commercial standard (lower). (Online version in colour.)

    Techniques Used: Gas Chromatography-Mass Spectrometry

    Antifungal activity test of soldier pheromone component. ( a ) Schematic drawing of fungal bioassay device. PDA, potato-dextrose agar. ( b – d ) Effects of (−)- β -elemene on the mycelial growth of fungi  B. bassiana  ( b ),  Me. anisopliae  ( c ) and termite ball fungi (TMB) ( d ). (−)- β -elemene suppressed the growth of entomopathogenic fungi  B. bassiana  and  Me. anisopliae , but enhanced that of TMB (repeated-measures ANOVA; * p
    Figure Legend Snippet: Antifungal activity test of soldier pheromone component. ( a ) Schematic drawing of fungal bioassay device. PDA, potato-dextrose agar. ( b – d ) Effects of (−)- β -elemene on the mycelial growth of fungi B. bassiana ( b ), Me. anisopliae ( c ) and termite ball fungi (TMB) ( d ). (−)- β -elemene suppressed the growth of entomopathogenic fungi B. bassiana and Me. anisopliae , but enhanced that of TMB (repeated-measures ANOVA; * p

    Techniques Used: Activity Assay

    Inhibitory effects of the crude soldier extract and the authentic (−)- β -elemene on soldier differentiation in the presence of JHA, hydroprene. Results show the average of cumulative rate of presoldiers after 15 days, and values denote the mean ± s.e.m. of 20 replications (10 replications × 2 colonies). Soldier extract and (−)- β -elemene suppressed the differentiation of new presoldiers. Different letters mean significant differences (Tukey's HSD test,  p
    Figure Legend Snippet: Inhibitory effects of the crude soldier extract and the authentic (−)- β -elemene on soldier differentiation in the presence of JHA, hydroprene. Results show the average of cumulative rate of presoldiers after 15 days, and values denote the mean ± s.e.m. of 20 replications (10 replications × 2 colonies). Soldier extract and (−)- β -elemene suppressed the differentiation of new presoldiers. Different letters mean significant differences (Tukey's HSD test, p

    Techniques Used:

    2) Product Images from "Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite"

    Article Title: Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite

    Journal: Proceedings of the Royal Society B: Biological Sciences

    doi: 10.1098/rspb.2017.1134

    Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- β -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p
    Figure Legend Snippet: Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- β -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p

    Techniques Used: Activity Assay

    GC–MS results. ( a ) Gas chromatographs of the whole-body extracts of both sexes of soldiers and workers from the same colony (UR160505A). The compound associated with a single large peak (indicated by an arrow) was identified as (−)- β -elemene (retention time: 16.41 min in male, 16.41 min in female). ( b ) Mass spectra of (−)- β -elemene from male soldiers (upper) and of commercial standard (lower). ( c ) Mass spectra of (−)- β -elemene from female soldiers (upper) and of commercial standard (lower). (Online version in colour.)
    Figure Legend Snippet: GC–MS results. ( a ) Gas chromatographs of the whole-body extracts of both sexes of soldiers and workers from the same colony (UR160505A). The compound associated with a single large peak (indicated by an arrow) was identified as (−)- β -elemene (retention time: 16.41 min in male, 16.41 min in female). ( b ) Mass spectra of (−)- β -elemene from male soldiers (upper) and of commercial standard (lower). ( c ) Mass spectra of (−)- β -elemene from female soldiers (upper) and of commercial standard (lower). (Online version in colour.)

    Techniques Used: Gas Chromatography-Mass Spectrometry

    Antifungal activity test of soldier pheromone component. ( a ) Schematic drawing of fungal bioassay device. PDA, potato-dextrose agar. ( b – d ) Effects of (−)- β -elemene on the mycelial growth of fungi B. bassiana ( b ), Me. anisopliae ( c ) and termite ball fungi (TMB) ( d ). (−)- β -elemene suppressed the growth of entomopathogenic fungi B. bassiana and Me. anisopliae , but enhanced that of TMB (repeated-measures ANOVA; * p
    Figure Legend Snippet: Antifungal activity test of soldier pheromone component. ( a ) Schematic drawing of fungal bioassay device. PDA, potato-dextrose agar. ( b – d ) Effects of (−)- β -elemene on the mycelial growth of fungi B. bassiana ( b ), Me. anisopliae ( c ) and termite ball fungi (TMB) ( d ). (−)- β -elemene suppressed the growth of entomopathogenic fungi B. bassiana and Me. anisopliae , but enhanced that of TMB (repeated-measures ANOVA; * p

    Techniques Used: Activity Assay

    Inhibitory effects of the crude soldier extract and the authentic (−)- β -elemene on soldier differentiation in the presence of JHA, hydroprene. Results show the average of cumulative rate of presoldiers after 15 days, and values denote the mean ± s.e.m. of 20 replications (10 replications × 2 colonies). Soldier extract and (−)- β -elemene suppressed the differentiation of new presoldiers. Different letters mean significant differences (Tukey's HSD test, p
    Figure Legend Snippet: Inhibitory effects of the crude soldier extract and the authentic (−)- β -elemene on soldier differentiation in the presence of JHA, hydroprene. Results show the average of cumulative rate of presoldiers after 15 days, and values denote the mean ± s.e.m. of 20 replications (10 replications × 2 colonies). Soldier extract and (−)- β -elemene suppressed the differentiation of new presoldiers. Different letters mean significant differences (Tukey's HSD test, p

    Techniques Used:

    Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- β -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p
    Figure Legend Snippet: Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- β -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p

    Techniques Used: Activity Assay

    GC–MS results. ( a ) Gas chromatographs of the whole-body extracts of both sexes of soldiers and workers from the same colony (UR160505A). The compound associated with a single large peak (indicated by an arrow) was identified as (−)- β -elemene (retention time: 16.41 min in male, 16.41 min in female). ( b ) Mass spectra of (−)- β -elemene from male soldiers (upper) and of commercial standard (lower). ( c ) Mass spectra of (−)- β -elemene from female soldiers (upper) and of commercial standard (lower). (Online version in colour.)
    Figure Legend Snippet: GC–MS results. ( a ) Gas chromatographs of the whole-body extracts of both sexes of soldiers and workers from the same colony (UR160505A). The compound associated with a single large peak (indicated by an arrow) was identified as (−)- β -elemene (retention time: 16.41 min in male, 16.41 min in female). ( b ) Mass spectra of (−)- β -elemene from male soldiers (upper) and of commercial standard (lower). ( c ) Mass spectra of (−)- β -elemene from female soldiers (upper) and of commercial standard (lower). (Online version in colour.)

    Techniques Used: Gas Chromatography-Mass Spectrometry

    Antifungal activity test of soldier pheromone component. ( a ) Schematic drawing of fungal bioassay device. PDA, potato-dextrose agar. ( b – d ) Effects of (−)- β -elemene on the mycelial growth of fungi  B. bassiana  ( b ),  Me. anisopliae  ( c ) and termite ball fungi (TMB) ( d ). (−)- β -elemene suppressed the growth of entomopathogenic fungi  B. bassiana  and  Me. anisopliae , but enhanced that of TMB (repeated-measures ANOVA; * p
    Figure Legend Snippet: Antifungal activity test of soldier pheromone component. ( a ) Schematic drawing of fungal bioassay device. PDA, potato-dextrose agar. ( b – d ) Effects of (−)- β -elemene on the mycelial growth of fungi B. bassiana ( b ), Me. anisopliae ( c ) and termite ball fungi (TMB) ( d ). (−)- β -elemene suppressed the growth of entomopathogenic fungi B. bassiana and Me. anisopliae , but enhanced that of TMB (repeated-measures ANOVA; * p

    Techniques Used: Activity Assay

    Inhibitory effects of the crude soldier extract and the authentic (−)- β -elemene on soldier differentiation in the presence of JHA, hydroprene. Results show the average of cumulative rate of presoldiers after 15 days, and values denote the mean ± s.e.m. of 20 replications (10 replications × 2 colonies). Soldier extract and (−)- β -elemene suppressed the differentiation of new presoldiers. Different letters mean significant differences (Tukey's HSD test,  p
    Figure Legend Snippet: Inhibitory effects of the crude soldier extract and the authentic (−)- β -elemene on soldier differentiation in the presence of JHA, hydroprene. Results show the average of cumulative rate of presoldiers after 15 days, and values denote the mean ± s.e.m. of 20 replications (10 replications × 2 colonies). Soldier extract and (−)- β -elemene suppressed the differentiation of new presoldiers. Different letters mean significant differences (Tukey's HSD test, p

    Techniques Used:

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  • 91
    Abcam β elemene
    Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- <t>β</t> -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p
    β Elemene, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β elemene/product/Abcam
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    β elemene - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    94
    Abcam ß elemene
    The growth rate of A375 cell line was inhibited by <t>ß-elemene.</t> A375 cells were cultivated in 96-well plates at a density of 5×103 cells/well and treated with different concentrations of ß-elemene in different time periods: A. 24, B. 48, and C. 72 hours. Cell proliferation was evaluated using the MTT assay. The IC 50 value is a concentration of a drug that inhibits cell proliferation by 50% in comparison to the control. The data are shown as the means ± SD of three independent experiments. Asterisks indicate significant differences. ****; P
    ß Elemene, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ß elemene/product/Abcam
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ß elemene - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- β -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p

    Journal: Proceedings of the Royal Society B: Biological Sciences

    Article Title: Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite

    doi: 10.1098/rspb.2017.1134

    Figure Lengend Snippet: Arrestant activity of soldier extract and artificial pheromone after 15 min. ( a ) Schematic drawing of behavioural bioassay device. ( b ) Workers were arrested by (−)- β -elemene at 1000 ng per disk as well as soldier extract (Dunnett's test; n.s., not significant; * p

    Article Snippet: To test the dose-dependency of β -elemene, a standard solution containing 0.1 mg of authentic standard of (−)- β -elemene (Abcam) diluted in 1 ml hexane was prepared, and this standard solution and 10- to 10 000-fold diluted hexane solutions of it were used.

    Techniques: Activity Assay

    GC–MS results. ( a ) Gas chromatographs of the whole-body extracts of both sexes of soldiers and workers from the same colony (UR160505A). The compound associated with a single large peak (indicated by an arrow) was identified as (−)- β -elemene (retention time: 16.41 min in male, 16.41 min in female). ( b ) Mass spectra of (−)- β -elemene from male soldiers (upper) and of commercial standard (lower). ( c ) Mass spectra of (−)- β -elemene from female soldiers (upper) and of commercial standard (lower). (Online version in colour.)

    Journal: Proceedings of the Royal Society B: Biological Sciences

    Article Title: Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite

    doi: 10.1098/rspb.2017.1134

    Figure Lengend Snippet: GC–MS results. ( a ) Gas chromatographs of the whole-body extracts of both sexes of soldiers and workers from the same colony (UR160505A). The compound associated with a single large peak (indicated by an arrow) was identified as (−)- β -elemene (retention time: 16.41 min in male, 16.41 min in female). ( b ) Mass spectra of (−)- β -elemene from male soldiers (upper) and of commercial standard (lower). ( c ) Mass spectra of (−)- β -elemene from female soldiers (upper) and of commercial standard (lower). (Online version in colour.)

    Article Snippet: To test the dose-dependency of β -elemene, a standard solution containing 0.1 mg of authentic standard of (−)- β -elemene (Abcam) diluted in 1 ml hexane was prepared, and this standard solution and 10- to 10 000-fold diluted hexane solutions of it were used.

    Techniques: Gas Chromatography-Mass Spectrometry

    Antifungal activity test of soldier pheromone component. ( a ) Schematic drawing of fungal bioassay device. PDA, potato-dextrose agar. ( b – d ) Effects of (−)- β -elemene on the mycelial growth of fungi B. bassiana ( b ), Me. anisopliae ( c ) and termite ball fungi (TMB) ( d ). (−)- β -elemene suppressed the growth of entomopathogenic fungi B. bassiana and Me. anisopliae , but enhanced that of TMB (repeated-measures ANOVA; * p

    Journal: Proceedings of the Royal Society B: Biological Sciences

    Article Title: Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite

    doi: 10.1098/rspb.2017.1134

    Figure Lengend Snippet: Antifungal activity test of soldier pheromone component. ( a ) Schematic drawing of fungal bioassay device. PDA, potato-dextrose agar. ( b – d ) Effects of (−)- β -elemene on the mycelial growth of fungi B. bassiana ( b ), Me. anisopliae ( c ) and termite ball fungi (TMB) ( d ). (−)- β -elemene suppressed the growth of entomopathogenic fungi B. bassiana and Me. anisopliae , but enhanced that of TMB (repeated-measures ANOVA; * p

    Article Snippet: To test the dose-dependency of β -elemene, a standard solution containing 0.1 mg of authentic standard of (−)- β -elemene (Abcam) diluted in 1 ml hexane was prepared, and this standard solution and 10- to 10 000-fold diluted hexane solutions of it were used.

    Techniques: Activity Assay

    Inhibitory effects of the crude soldier extract and the authentic (−)- β -elemene on soldier differentiation in the presence of JHA, hydroprene. Results show the average of cumulative rate of presoldiers after 15 days, and values denote the mean ± s.e.m. of 20 replications (10 replications × 2 colonies). Soldier extract and (−)- β -elemene suppressed the differentiation of new presoldiers. Different letters mean significant differences (Tukey's HSD test, p

    Journal: Proceedings of the Royal Society B: Biological Sciences

    Article Title: Multi-functional roles of a soldier-specific volatile as a worker arrestant, primer pheromone and an antimicrobial agent in a termite

    doi: 10.1098/rspb.2017.1134

    Figure Lengend Snippet: Inhibitory effects of the crude soldier extract and the authentic (−)- β -elemene on soldier differentiation in the presence of JHA, hydroprene. Results show the average of cumulative rate of presoldiers after 15 days, and values denote the mean ± s.e.m. of 20 replications (10 replications × 2 colonies). Soldier extract and (−)- β -elemene suppressed the differentiation of new presoldiers. Different letters mean significant differences (Tukey's HSD test, p

    Article Snippet: To test the dose-dependency of β -elemene, a standard solution containing 0.1 mg of authentic standard of (−)- β -elemene (Abcam) diluted in 1 ml hexane was prepared, and this standard solution and 10- to 10 000-fold diluted hexane solutions of it were used.

    Techniques:

    The growth rate of A375 cell line was inhibited by ß-elemene. A375 cells were cultivated in 96-well plates at a density of 5×103 cells/well and treated with different concentrations of ß-elemene in different time periods: A. 24, B. 48, and C. 72 hours. Cell proliferation was evaluated using the MTT assay. The IC 50 value is a concentration of a drug that inhibits cell proliferation by 50% in comparison to the control. The data are shown as the means ± SD of three independent experiments. Asterisks indicate significant differences. ****; P

    Journal: Cell Journal (Yakhteh)

    Article Title: The Use of ß-Elemene to Enhance Radio Sensitization of A375 Human Melanoma Cells

    doi: 10.22074/cellj.2020.6326

    Figure Lengend Snippet: The growth rate of A375 cell line was inhibited by ß-elemene. A375 cells were cultivated in 96-well plates at a density of 5×103 cells/well and treated with different concentrations of ß-elemene in different time periods: A. 24, B. 48, and C. 72 hours. Cell proliferation was evaluated using the MTT assay. The IC 50 value is a concentration of a drug that inhibits cell proliferation by 50% in comparison to the control. The data are shown as the means ± SD of three independent experiments. Asterisks indicate significant differences. ****; P

    Article Snippet: ß-elemene was purchased from Abcam (Abcam, USA).

    Techniques: MTT Assay, Concentration Assay

    The capability of ß-elemene to inhibit cell proliferation was measured by the MTT assay. The viability of cells was approximately decreased in dose- and time-dependent manners. The difference in the IC 50 value for ß-elemene was observed among the different incubation times. A significant reduction was detected in the viability of treated cells in a 72 hours incubation time compared with 24 and 48 hour periods. The data are presented as the means ± SD of three independent experiments. IC 50 ; The half maximal inhibitory concentration.

    Journal: Cell Journal (Yakhteh)

    Article Title: The Use of ß-Elemene to Enhance Radio Sensitization of A375 Human Melanoma Cells

    doi: 10.22074/cellj.2020.6326

    Figure Lengend Snippet: The capability of ß-elemene to inhibit cell proliferation was measured by the MTT assay. The viability of cells was approximately decreased in dose- and time-dependent manners. The difference in the IC 50 value for ß-elemene was observed among the different incubation times. A significant reduction was detected in the viability of treated cells in a 72 hours incubation time compared with 24 and 48 hour periods. The data are presented as the means ± SD of three independent experiments. IC 50 ; The half maximal inhibitory concentration.

    Article Snippet: ß-elemene was purchased from Abcam (Abcam, USA).

    Techniques: MTT Assay, Incubation, Concentration Assay

    Cell proliferation was inhibited by ß-elemene. ß-elemene also increased the radiosensitivity of A375 cells. Comparison of the viability of A375 cells after the treatment with 40, 80 µg/ml of ß-elemene. After 24 hours of incubation time, cells were exposed to 2 and 4 (Gy) of 6 MV X-ray; then, the viability of cells was measured using the MTT assay. All treated groups were compared with the control group (treatment-naive). The data are presented as the means ± SD of three independent experiments. Asterisks indicate significant difference. *; P

    Journal: Cell Journal (Yakhteh)

    Article Title: The Use of ß-Elemene to Enhance Radio Sensitization of A375 Human Melanoma Cells

    doi: 10.22074/cellj.2020.6326

    Figure Lengend Snippet: Cell proliferation was inhibited by ß-elemene. ß-elemene also increased the radiosensitivity of A375 cells. Comparison of the viability of A375 cells after the treatment with 40, 80 µg/ml of ß-elemene. After 24 hours of incubation time, cells were exposed to 2 and 4 (Gy) of 6 MV X-ray; then, the viability of cells was measured using the MTT assay. All treated groups were compared with the control group (treatment-naive). The data are presented as the means ± SD of three independent experiments. Asterisks indicate significant difference. *; P

    Article Snippet: ß-elemene was purchased from Abcam (Abcam, USA).

    Techniques: Incubation, MTT Assay

    Annexin V-PI staining for the assessment of apoptosis following ß-elemene and radiation therapy in A375 human melanoma cell line. The pretreated process on cancer cells was performed at two concentrations of ß-elemene (40 and 80 µg/ml) for 24 hours. Then cells were exposed to 2 and 4 Gy irradiations in combination with ß-elemene for 24 hours. A. Early apoptosis was evaluated by Annexin V+/PI- staining, and Annexin V+/PI+ staining was applied as a marker for the detection of cells in the late apoptosis phase and B. PI and Annexin V double staining results indicated the induction of apoptosis by ß-elemene and enhanced radiation-induced apoptosis in human melanoma cancer cells. Cells were exposed to ß-elemene at concentrations of 40 and 80 µg/ml along with 2 and 4 Gy irradiations. All the treatment groups were compared with the control group (no drug). The data are presented as the means ± SD of three independent experiments. Asterisks indicate significant differences. ****; P

    Journal: Cell Journal (Yakhteh)

    Article Title: The Use of ß-Elemene to Enhance Radio Sensitization of A375 Human Melanoma Cells

    doi: 10.22074/cellj.2020.6326

    Figure Lengend Snippet: Annexin V-PI staining for the assessment of apoptosis following ß-elemene and radiation therapy in A375 human melanoma cell line. The pretreated process on cancer cells was performed at two concentrations of ß-elemene (40 and 80 µg/ml) for 24 hours. Then cells were exposed to 2 and 4 Gy irradiations in combination with ß-elemene for 24 hours. A. Early apoptosis was evaluated by Annexin V+/PI- staining, and Annexin V+/PI+ staining was applied as a marker for the detection of cells in the late apoptosis phase and B. PI and Annexin V double staining results indicated the induction of apoptosis by ß-elemene and enhanced radiation-induced apoptosis in human melanoma cancer cells. Cells were exposed to ß-elemene at concentrations of 40 and 80 µg/ml along with 2 and 4 Gy irradiations. All the treatment groups were compared with the control group (no drug). The data are presented as the means ± SD of three independent experiments. Asterisks indicate significant differences. ****; P

    Article Snippet: ß-elemene was purchased from Abcam (Abcam, USA).

    Techniques: Staining, Marker, Double Staining

    The growth rate of A375 cell line was inhibited by ß-elemene. A375 cells were cultivated in 96-well plates at a density of 5×103 cells/well and treated with different concentrations of ß-elemene in different time periods: A. 24, B. 48, and C. 72 hours. Cell proliferation was evaluated using the MTT assay. The IC 50 value is a concentration of a drug that inhibits cell proliferation by 50% in comparison to the control. The data are shown as the means ± SD of three independent experiments. Asterisks indicate significant differences. ****; P

    Journal: Cell Journal (Yakhteh)

    Article Title: The Use of ß-Elemene to Enhance Radio Sensitization of A375 Human Melanoma Cells

    doi: 10.22074/cellj.2020.6326

    Figure Lengend Snippet: The growth rate of A375 cell line was inhibited by ß-elemene. A375 cells were cultivated in 96-well plates at a density of 5×103 cells/well and treated with different concentrations of ß-elemene in different time periods: A. 24, B. 48, and C. 72 hours. Cell proliferation was evaluated using the MTT assay. The IC 50 value is a concentration of a drug that inhibits cell proliferation by 50% in comparison to the control. The data are shown as the means ± SD of three independent experiments. Asterisks indicate significant differences. ****; P

    Article Snippet: Agents ß-elemene was purchased from Abcam (Abcam, USA).

    Techniques: MTT Assay, Concentration Assay

    The capability of ß-elemene to inhibit cell proliferation was measured by the MTT assay. The viability of cells was approximately decreased in dose- and time-dependent manners. The difference in the IC 50 value for ß-elemene was observed among the different incubation times. A significant reduction was detected in the viability of treated cells in a 72 hours incubation time compared with 24 and 48 hour periods. The data are presented as the means ± SD of three independent experiments. IC 50 ; The half maximal inhibitory concentration.

    Journal: Cell Journal (Yakhteh)

    Article Title: The Use of ß-Elemene to Enhance Radio Sensitization of A375 Human Melanoma Cells

    doi: 10.22074/cellj.2020.6326

    Figure Lengend Snippet: The capability of ß-elemene to inhibit cell proliferation was measured by the MTT assay. The viability of cells was approximately decreased in dose- and time-dependent manners. The difference in the IC 50 value for ß-elemene was observed among the different incubation times. A significant reduction was detected in the viability of treated cells in a 72 hours incubation time compared with 24 and 48 hour periods. The data are presented as the means ± SD of three independent experiments. IC 50 ; The half maximal inhibitory concentration.

    Article Snippet: Agents ß-elemene was purchased from Abcam (Abcam, USA).

    Techniques: MTT Assay, Incubation, Concentration Assay

    Cell proliferation was inhibited by ß-elemene. ß-elemene also increased the radiosensitivity of A375 cells. Comparison of the viability of A375 cells after the treatment with 40, 80 µg/ml of ß-elemene. After 24 hours of incubation time, cells were exposed to 2 and 4 (Gy) of 6 MV X-ray; then, the viability of cells was measured using the MTT assay. All treated groups were compared with the control group (treatment-naive). The data are presented as the means ± SD of three independent experiments. Asterisks indicate significant difference. *; P

    Journal: Cell Journal (Yakhteh)

    Article Title: The Use of ß-Elemene to Enhance Radio Sensitization of A375 Human Melanoma Cells

    doi: 10.22074/cellj.2020.6326

    Figure Lengend Snippet: Cell proliferation was inhibited by ß-elemene. ß-elemene also increased the radiosensitivity of A375 cells. Comparison of the viability of A375 cells after the treatment with 40, 80 µg/ml of ß-elemene. After 24 hours of incubation time, cells were exposed to 2 and 4 (Gy) of 6 MV X-ray; then, the viability of cells was measured using the MTT assay. All treated groups were compared with the control group (treatment-naive). The data are presented as the means ± SD of three independent experiments. Asterisks indicate significant difference. *; P

    Article Snippet: Agents ß-elemene was purchased from Abcam (Abcam, USA).

    Techniques: Incubation, MTT Assay

    Annexin V-PI staining for the assessment of apoptosis following ß-elemene and radiation therapy in A375 human melanoma cell line. The pretreated process on cancer cells was performed at two concentrations of ß-elemene (40 and 80 µg/ml) for 24 hours. Then cells were exposed to 2 and 4 Gy irradiations in combination with ß-elemene for 24 hours. A. Early apoptosis was evaluated by Annexin V+/PI- staining, and Annexin V+/PI+ staining was applied as a marker for the detection of cells in the late apoptosis phase and B. PI and Annexin V double staining results indicated the induction of apoptosis by ß-elemene and enhanced radiation-induced apoptosis in human melanoma cancer cells. Cells were exposed to ß-elemene at concentrations of 40 and 80 µg/ml along with 2 and 4 Gy irradiations. All the treatment groups were compared with the control group (no drug). The data are presented as the means ± SD of three independent experiments. Asterisks indicate significant differences. ****; P

    Journal: Cell Journal (Yakhteh)

    Article Title: The Use of ß-Elemene to Enhance Radio Sensitization of A375 Human Melanoma Cells

    doi: 10.22074/cellj.2020.6326

    Figure Lengend Snippet: Annexin V-PI staining for the assessment of apoptosis following ß-elemene and radiation therapy in A375 human melanoma cell line. The pretreated process on cancer cells was performed at two concentrations of ß-elemene (40 and 80 µg/ml) for 24 hours. Then cells were exposed to 2 and 4 Gy irradiations in combination with ß-elemene for 24 hours. A. Early apoptosis was evaluated by Annexin V+/PI- staining, and Annexin V+/PI+ staining was applied as a marker for the detection of cells in the late apoptosis phase and B. PI and Annexin V double staining results indicated the induction of apoptosis by ß-elemene and enhanced radiation-induced apoptosis in human melanoma cancer cells. Cells were exposed to ß-elemene at concentrations of 40 and 80 µg/ml along with 2 and 4 Gy irradiations. All the treatment groups were compared with the control group (no drug). The data are presented as the means ± SD of three independent experiments. Asterisks indicate significant differences. ****; P

    Article Snippet: Agents ß-elemene was purchased from Abcam (Abcam, USA).

    Techniques: Staining, Marker, Double Staining